Alok K Mishra, Tianyi Ye, Shahid Banday, Ritesh P Thakare, Chinh Tran-To Su, Ngoc N H Pham, Amjad Ali, Ankur Kulshreshtha, Shreya Roy Chowdhury, Tessa M Simone, Kai Hu, Lihua Julie Zhu, Birgit Eisenhaber, Sara K Deibler, Karl Simin, Paul R Thompson, Michelle A Kelliher, Frank Eisenhaber, Sunil K Malonia, Michael R Green
CD24 is frequently overexpressed in ovarian cancer and promotes immune evasion by interacting with its receptor Siglec10, present on tumor-associated macrophages, providing a "don't eat me" signal that prevents targeting and phagocytosis by macrophages. Factors promoting CD24 expression could represent novel immunotherapeutic targets for ovarian cancer. Here, using a genome-wide CRISPR knockout screen, we identify GPAA1 (glycosylphosphatidylinositol anchor attachment 1), a factor that catalyzes the attachment of a glycosylphosphatidylinositol (GPI) lipid anchor to substrate proteins, as a positive regulator of CD24 cell surface expression...
April 3, 2024: Cell Reports