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Frédérique Eyrolle, Loïc Ducros, Séverine Le Dizès, Karine Beaugelin-Seiller, Sabine Charmasson, Patrick Boyer, Catherine Cossonnet
Various studies indicated more or less recently that organically bound tritium (OBT) formed from gaseous or liquid tritium releases into the environment potentially accumulates in organisms contradicting hypotheses associated to methods used to assess the biological impact of tritium on humans (ASN, 2010). Increasing research works were then performed during the last decade in order to gain knowledge on this radionuclide expected to be increasingly released by nuclear installations in the near future within the environment...
November 14, 2017: Journal of Environmental Radioactivity
Wendy F Li, Heidi Pollard, Mohsen Karimi, Jeremy D Asnes, William E Hellenbrand, Veronika Shabanova, Constance G Weismann
OBJECTIVE: Trans-catheter (TC) pulmonary valve replacement (PVR) has become common practice for patients with right ventricular outflow tract obstruction (RVOTO) and/or pulmonic insufficiency (PI). Our aim was to compare PVR and right ventricular (RV) function of patients who received TC vs surgical PVR. DESIGN: Retrospective review of echocardiograms obtained at three time points: before, immediately after PVR, and most recent. PATIENTS: Sixty-two patients (median age 19 years, median follow-up 25 months) following TC (N = 32) or surgical (N = 30) PVR at Yale-New Haven Hospital were included...
November 17, 2017: Congenital Heart Disease
Bo Lv, Hanli Sun, Shen Huang, Xudong Feng, Tao Jiang, Chun Li
Glycoside hydrolases (GHs) have attracted special attention in research aimed at modifying natural products by partial removal of sugar moieties to manipulate their solubility and efficacy. However, these modifications are challenging to control because the low substrate specificity of most GHs often generates undesired by-products. We previously identified a GH2-type fungal β-glucuronidase from Aspergillus oryzae (PGUS) exhibiting promiscuous substrate specificity in hydrolysis of triterpenoid saponins. Here, we present the PGUS structure, representing the first structure of a fungal β-glucuronidase, and of an inactive PGUS mutant in complex with the native substrate glycyrrhetic acid 3-O-mono-β- glucuronide (GAMG)...
November 16, 2017: Journal of Biological Chemistry
Qing Shi, Ryugo Hashimoto, Tadamune Otsubo, Kiyoshi Ikeda, Kenichiro Todoroki, Hajime Mizuno, Dongri Jin, Toshimasa Toyo'oka, Zhe Jiang, Jun Zhe Min
The lack of a highly sensitive and simple method for the quantitative analysis of glycan has impeded the exploration of protein glycosylation patterns (glycomics), evaluation of antibody drug stability, and screening of disease glycan biomarkers. In this study, we describe a novel and simplified quantitative glycomics strategy. Quantitation by mutant enzyme reaction stable isotope labeling (QMERSIL) to label the N-glycans with either a nondeuterated (d0-) or deuterated (d8-) 4-(2,4-Dinitro-5-piperazin-1-yl-phenyl)-1,1-dimethyl-piperazin-1-ium (MPDPZ)-Boc-asparaginyl-N-acetyl-d-glucosamine (Boc-Asn-GlcNAc) acceptor of a positive charge structure through the glycosynthase (Endo-M-N175Q) transglycosylation reaction with mass spectrometry facilitates comparative glycomics...
November 12, 2017: Journal of Pharmaceutical and Biomedical Analysis
Ambure Sharadadevi, Ramakrishnan Nagaraj
No abstract text is available yet for this article.
November 15, 2017: Journal of Biomolecular Structure & Dynamics
Xin-Jun Cai, Zeng Wang, Jia-Wei Cao, Jian-Jun Ni, Ying-Ying Xu, Jun Yao, Hong Xu, Fang Liu, Gao-Yi Yang
Zoledronic acid (ZOL) has been used as an adjuvant therapy for breast cancer. It is suggested that ZOL might be associated with inhibition of macrophages, which in turn reduces tumor growth, metastasis and tumor angiogenesis. Moreover, metronomic therapy can inhibit tumor angiogenesis and tumor immune cells. Previously we developed ZOL based cationic liposomes that allowed a higher intratumor delivery of drug compared with free ZOL in vivo. Therefore, in this study, Asn-Gly-Arg (NGR) and PEG2000 were used as ligands to modify the surface of liposomes (NGR-PEG-LP-ZOL) in metronomic therapy to clear the tumor-associated macrophages (TAMs) and inhibit the formation of tumor angiogenesis, achieving the purpose of anti-tumor growth...
October 13, 2017: Oncotarget
Hong-Lian Li, Ying Ma, Chang-Jie Zheng, Wen-Yan Jin, Wen-Shan Liu, Run-Ling Wang
Noonan syndrome (NS) is a common autosomal dominant congenital disorder which could cause the congenital cardiopathy and cancer predisposition. Previous studies reported that the knock-in mouse models of the mutant D61G of SHP2 exhibited the major features of NS, which demonstrated that the mutation D61G of SHP2 could cause NS. To explore the effect of D61G mutation on SHP2 and explain the high activity of the mutant, molecular dynamic (MD) simulations were performed on wild type (WT) of SHP2 and the mutated SHP2-D61G, respectively...
November 10, 2017: Journal of Biomolecular Structure & Dynamics
Andrew S Allegretti, Xavier Vela Parada, Nwamaka D Eneanya, Hannah Gilligan, Dihua Xu, Sophia Zhao, Jules L Dienstag, Raymond T Chung, Ravi I Thadhani
BACKGROUND AND OBJECTIVES: Literature on the prognosis of patients with cirrhosis who require RRT for AKI is sparse and is confounded by liver transplant eligibility. An update on outcomes in the nonlisted subgroup is needed. Our objective was to compare outcomes in this group between those diagnosed with hepatorenal syndrome and acute tubular necrosis, stratifying by liver transplant listing status. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Retrospective cohort study of patients with cirrhosis acutely initiated on hemodialysis or continuous RRT at five hospitals, including one liver transplant center...
November 9, 2017: Clinical Journal of the American Society of Nephrology: CJASN
Yuyu Chen, Kaimin Lu, Jianzong Li, Danfeng Liang, Hao Luo, Xiaoyun Wang, Xin Wang, Jinku Bao
The crystal structure of mature Polygonatum cyrtonema lectin (PCL) showed three similar carbohydrate-binding sites (CBS I, CBS II, and CBS III). The Gln58 and Asp60 residues of CBS II are substituted with His58 and Asn60. To establish the relationship between the key amino acid residues and structure or activity of PCL, we constructed four recombinant mutants in CBS I, CBS II, and CBS III. The experimental results indicate that CBS I, CBS III and the disulfide bond play vital roles in the binding with mannose...
November 7, 2017: Acta Biochimica et Biophysica Sinica
Subhanip Biswas, Chantal V Garcia De Gonzalo, Lindsay M Repka, Wilfred A van der Donk
Sublancin is a 37-amino acid antimicrobial peptide belonging to the glycocin family of natural products. It contains two helices that are held together by two disulfide bonds as well as an unusual S-glucosidic linkage to a Cys in a loop connecting the helices. We report the reconstitution of the biosynthetic pathway to this natural product in Escherichia coli. This technology enabled the evaluation of the structure-activity relationships of the solvent-exposed residues in the helices. The biosynthetic machinery proved tolerant of changes in both helices, and the bioactivity studies of the resulting mutants show that two residues in helix B are important for bioactivity, Asn31 and Arg33...
November 10, 2017: ACS Chemical Biology
Ping Kang, Yulan Liu, Huiling Zhu, Jing Zhang, Haifeng Shi, Shuang Li, Dingan Pi, Weibo Leng, Xiuying Wang, Huanting Wu, Yongqing Hou
Objective: This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in the weaning pigs when challenged with lipopolysaccharide. Methods: Forty-eight weaned pigs (Duroc × Large White × Landrace, 8.12±0.56 kg) were assigned to four treatments: 1) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; 2) LPSCC, piglets received the same control diet and injected with Escherichia coli LPS; 3) LPS + 0...
November 3, 2017: Asian-Australasian Journal of Animal Sciences
Xiuyun Wu, Zhennan Tian, Xukai Jiang, Qun Zhang, Lushan Wang
XynB from Aspergillus niger ATCC1015 (AnXynB) is a mesophilic glycoside hydrolase (GH) family 11 xylanase which holds great potentials in a wide variety of industrial applications. In the present study, the catalytic activity and stability of AnXynB were improved by a combination of computational and experimental approaches. Virtual mutation and molecular dynamics simulations indicated that the introduction of Glu and Asn altered the interaction network at the - 3 subsite. Interestingly, the double mutant S41N/T43E displayed 72% increase in catalytic activity when compared to the wild type (WT)...
November 4, 2017: Applied Microbiology and Biotechnology
Harald Rouha, Susanne Weber, Philipp Janesch, Barbara Maierhofer, Karin Gross, Ivana Dolezilkova, Irina Mirkina, Zehra C Visram, Stefan Malafa, Lukas Stulik, Adriana Badarau, Eszter Nagy
Pathogenesis of Staphylococcus aureus is increasingly recognized to be driven by powerful toxins. Staphylococcus aureus employs up to six pore-forming toxins to subvert the human host defense and to promote bacterial invasion: alpha-hemolysin that disrupts epithelial and endothelial barriers and five leukocidins that lyse phagocytes involved in bacterial clearance. Previously, we described two human monoclonal antibodies (mAbs), ASN-1 that neutralizes alpha-hemolysin and four leukocidins (LukSF-PV, LukED, HlgAB, HlgCB), and ASN-2 that inactivates the 5(th) leukocidin, LukGH...
November 3, 2017: Virulence
Yu Wang, Ting Zhang, Hongyi Zhang, Haixia Yang, Yanju Li, Yiqun Jiang
Pancreatic cancer is a digestive system malignant carcinoma with poor prognosis. The majority of patients are diagnosed with metastatic disease, which is also the leading cause of pancreatic cancer death. The aim of this study was to investigate the antimetastatic effect of Asn-Phe-Gly-Lys (NFGK), a tetrapeptide derived from bovine hemoglobin pepsin hydrolysate, on human pancreatic cancer cell line MIAPaCa-2. Wound healing assay and transwell invasion assay results showed that NFGK inhibited MIAPaCa-2 cell migration and invasion dose-dependently...
October 30, 2017: Journal of Food Science
Soniya S Rao, Libero J Bartolotti, Shridhar P Gejji
Mixtures of ionic liquids formed by blending a common 1-methyl-3-butylimidazolium [Bmim] cation with the dicarboxylic amino acid anions viz., aspartic acid [Asp], asparagine [Asn], glutamic acid [Glu], and glutamine [Gln], have been investigated by employing dispersion corrected density functional theory. Binary mixtures of [Bmim]2[Asp][Asn] and [Bmim]2[Glu][Gln] ionic liquids emerge with distinct structural patterns. Competition between the constituting anions towards cationic binding sites in acidic and basic (polar) amino acid binary mixtures engenders diverse noncovalent interactions, viz...
November 8, 2017: Physical Chemistry Chemical Physics: PCCP
Abul Kalam Azad, Jahed Ahmed, Md Asraful Alum, Md Mahbub Hasan, Takahiro Ishikawa, Yoshihiro Sawa
Major intrinsic proteins (MIPs), commonly known as aquaporins, transport water and non-polar small solutes. Comparing the 3D models and the primary selectivity-related motifs (two Asn-Pro-Ala (NPA) regions, the aromatic/arginine (ar/R) selectivity filter, and Froger's positions (FPs)) of all plant MIPs that have been experimentally proven to transport arsenic (As) and antimony (Sb), some substrate-specific signature sequences (SSSS) or specificity determining sites (SDPs) have been predicted. These SSSS or SDPs were determined in 543 MIPs found in the genomes of 12 crop plants; the As and Sb transporters were predicted to be distributed in noduline-26 like intrinsic proteins (NIPs), and every plant had one or several As and Sb transporter NIPs...
October 26, 2017: International Journal of Biological Macromolecules
Magdalena M Felczak, Sundari Chodavarapu, Jon M Kaguni
Former studies relying on hydrogen/deuterium exchange analysis suggest that DnaC bound to DnaB alters the conformation of the N-terminal domain (NTD) of DnaB to impair the ability of this DNA helicase to interact with primase. Supporting this idea, the work described herein based on biosensor experiments and enzyme-linked immunosorbent assays shows that the DnaB-DnaC complex binds poorly to primase in comparison with DnaB alone. Using a structural model of DnaB complexed with the C-terminal domain (CTD) of primase, we found that Ile-85 is located at the interface in the NTD of DnaB that contacts primase...
October 25, 2017: Journal of Biological Chemistry
Francesco Bonì, Mario Milani, Alberto Barbiroli, Luisa Diomede, Eloise Mastrangelo, Matteo de Rosa
AGel amyloidosis is a genetic degenerative disease characterized by the deposition of insoluble gelsolin protein aggregates in different tissues. Until recently, this disease was associated with two mutations of a single residue (Asp187 to Asn/Tyr) in the second domain of the protein. The general opinion is that pathogenic variants are not per se amyloidogenic but rather that the mutations trigger an aberrant proteolytic cascade, which results in the production of aggregation prone fragments. Here we report the crystal structure of the second domain of gelsolin carrying the recently identified Gly167Arg mutation...
October 23, 2017: Human Molecular Genetics
Gaddafi I Danmaliki, Philip B Liu, Peter M Hwang
Perdeuteration with selective (1)H,(13)C-enrichment of methyl groups has enabled solution NMR studies of large (>30 kDa) protein systems. However, we propose that for all non-methyl positions, only magnetization originating from (1)H-(12)C groups is sufficiently long-lived, and it can be transferred via through-space NOEs to slowly relaxing (1)H-(15)N or (1)H-(13)C methyl groups to achieve multidimensional solution NMR. We demonstrate stereoselective (1)H,(12)C-labeling by adding relatively inexpensive unlabeled carbon sources to Escherichia coli growth media in D2O...
November 3, 2017: Biochemistry
Christopher J Williams, Jeffrey J Headd, Nigel W Moriarty, Michael G Prisant, Lizbeth L Videau, Lindsay N Deis, Vishal Verma, Daniel A Keedy, Bradley J Hintze, Vincent B Chen, Swati Jain, Steven M Lewis, W Bryan Arendall, Jack Snoeyink, Paul D Adams, Simon C Lovell, Jane S Richardson, David C Richardson
This paper describes the current update on macromolecular model validation services that are provided at the MolProbity website, emphasizing changes and additions since the previous review in 2010. There have been many infrastructure improvements, including rewrite of previous Java utilities to now use existing or newly written Python utilities in the open-source CCTBX portion of the Phenix software system. This improves long-term maintainability and enhances the thorough integration of MolProbity-style validation within Phenix...
October 25, 2017: Protein Science: a Publication of the Protein Society
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