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https://www.readbyqxmd.com/read/28933546/rational-design-of-selective-adenine-based-scaffolds-for-inactivation-of-bacterial-histidine-kinases
#1
Manibarsha Goswami, Kaelyn E Wilke, Erin E Carlson
Bacterial histidine kinases (HKs) are quintessential regulatory enzymes found ubiquitously in bacteria. Apart from regulatory roles, they are also involved in the production of virulence factors and conferring resistance to various antibiotics in pathogenic microbes. We have previously reported compounds that inhibit multiple HKs by targeting the conserved catalytic and ATP-binding (CA) domain. Herein, we conduct a detailed structure-activity relationship assessment of adenine-based inhibitors using biochemical and docking methods...
September 21, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28932390/newest-data-on-fluoroquinolone-resistance-mechanism-of-shigella-flexneri-isolates-in-jiangsu-province-of-china
#2
Tingting Qin, Huimin Qian, Wenting Fan, Ping Ma, Lu Zhou, Chen Dong, Bing Gu, Xiang Huo
BACKGROUND: To determine the prevalence, antimicrobial susceptibility patterns and related presence of mutations in quinolone resistance-determining region (QRDR) genes and plasmid-mediated quinolone resistance (PMQR) among Shigella flexneri isolates obtained from Jiangsu Province, China. METHODS: A total of 400 Shigella flexneri clinical isolates collected during 2012-2015 were identified by biochemical and serological methods, and the antimicrobial susceptibility pattern was evaluated using the disc-diffusion method...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28927484/validation-of-telephone-based-behavioral-assessments-in-aging-services-clients
#3
Yeates Conwell, Adam Simning, Nicole Driffill, Yinglin Xia, Xin Tu, Susan P Messing, David Oslin
BACKGROUND: The Behavioral Health Laboratory (BHL), a telephone-based mental health assessment, is a cost-effective approach that can improve mental illness identification and management. The individual BHL instruments, which were originally designed to be administered in-person, have not yet been validated with an in-person BHL assessment. This study therefore aims to characterize the concordance between the BHL data gathered by telephone and in-person interviews. METHODS: A cross-sectional study was conducted with English-speaking aging services network (ASN) clients aged 60 years and older in Monroe County, NY who were randomized to a BHL interview either in-person (n = 55) or by telephone (n = 53)...
September 20, 2017: International Psychogeriatrics
https://www.readbyqxmd.com/read/28923833/nephrology-provider-prognostic-perceptions-and-care-delivered-to-older-adults-with-advanced-kidney-disease
#4
Huzaifah Salat, Andrei Javier, Edward D Siew, Rocio Figueroa, Loren Lipworth, Edmond Kabagambe, Aihua Bian, Thomas G Stewart, Maie H El-Sourady, Mohana Karlekar, Cesar Y Cardona, T Alp Ikizler, Khaled Abdel-Kader
BACKGROUND AND OBJECTIVES: Prognostic uncertainty is one barrier that impedes providers in engaging patients with CKD in shared decision making and advance care planning. The surprise question has been shown to identify patients at increased risk of dying. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: In our prospective observational study, 488 patients ≥60 years of age with CKD stage 4 or 5 were enrolled. Binary surprise question (i.e., "Would you be surprised if this patient died in the next 12 months?") responses were recorded, and dialysis planning preferences, presence of advance care planning documentation, and care preceding death were abstracted...
September 18, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28921735/three-shades-of-grey-detecting-brain-abnormalities-in-children-with-autism-by-using-source-voxel-and-surface-based-morphometry
#5
Edoardo Pappaianni, Roma Siugzdaite, Sofie Vettori, Paola Venuti, Remo Job, Alessandro Grecucci
Autistic spectrum disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social interactions, communication and stereotyped behavior. Recent evidence from neuroimaging supports the hypothesis that ASD deficits in adults may be related to abnormalities in a specific frontal-temporal network (Autism-specific Structural Network, ASN). To see whether these results extend to younger children and to better characterize these abnormalities, we applied three morphometric methods on brain grey matter of children with and without ASD...
September 16, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28920676/changes-in-amino-acid-profile-in-roots-of-glyphosate-resistant-and-susceptible-soybean-glycine-max-induced-by-foliar-glyphosate-application
#6
Carlos Alberto Moldes, Miguel Angel Cantarelli, José Manuel Camiña, Siu Mui Tsai, Ricardo A Azevedo
Amino acid profiles are useful to analyze the responses to glyphosate in susceptible and resistant soybean lines. Comparisons of profiles for 10 amino acids (Asp, Asn, Glu, Gln, Ser, His, Gly, Thr, Tyr, Leu) by HPLC in soybean roots were performed in two near isogenic pairs (four varieties). Foliar application of glyphosate was applied in soybean plants after 5 weeks of seeding. Roots of four varieties were collected at 0 and 72 h after glyphosate application (AGA) for amino acids analysis by HPLC. Univariate analysis showed a significant increasing of several amino acids in susceptible as well as resistant soybean lines; however, amino acids from carbon (C) and nitrogen (N) metabolism, such as Asp, Asn, Glu and Gln, and Ser, increased significantly in susceptible varieties at 72 h AGA...
September 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28910155/identification-of-the-molecular-mechanism-of-trimethoprim-resistance-in-listeria-monocytogenes
#7
Dorota Korsak, Agata Krawczyk-Balska
Trimethoprim with sulfamethoxazole is a therapeutic agent combination used to treat infections caused by the facultative intracellular foodborne pathogen Listeria monocytogenes. The aim of this study was to assess the frequency of resistance of L. monocytogenes arising due to exposure to trimethoprim and subsequently investigate the molecular mechanisms of resistance. After exposure of a culture of L. monocytogenes ATCC 13932 to trimethoprim at 10-fold the minimal inhibitory concentration spontaneous resistant mutants were recovered, giving a frequency of resistance development of 6...
September 14, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28910115/bulky-dehydroamino-acids-enhance-proteolytic-stability-and-folding-in-%C3%AE-hairpin-peptides
#8
Ankur Jalan, David W Kastner, Kei G I Webber, Mason S Smith, Joshua L Price, Steven L Castle
The bulky dehydroamino acids dehydrovaline (ΔVal) and dehydroethylnorvaline (ΔEnv) can be inserted into the turn regions of β-hairpin peptides without altering their secondary structures. These residues increase proteolytic stability, with ΔVal at the (i + 1) position having the most substantial impact. Additionally, a bulky dehydroamino acid can be paired with a d-amino acid (i.e., d-Pro) to synergistically enhance resistance to proteolysis. A link between proteolytic stability and peptide structure is established by the finding that a stabilized ΔVal-containing β-hairpin is more highly folded than its Asn-containing congener...
September 14, 2017: Organic Letters
https://www.readbyqxmd.com/read/28892050/carcinoembryonic-antigen-related-cell-adhesion-molecule-6-ceacam6-promotes-egf-receptor-signaling-of-oral-squamous-cell-carcinoma-metastasis-via-the-complex-n-glycosylation
#9
W-F Chiang, T-M Cheng, C-C Chang, S-H Pan, C A Changou, T-H Chang, K-H Lee, S-Y Wu, Y-F Chen, K-H Chuang, D-B Shieh, Y-L Chen, C-C Tu, W-L Tsui, M-H Wu
Aberrant protein glycosylation could be a distinct surface-marker of cancer cells that influences cancer progression and metastasis because glycosylation can regulate membrane protein folding which alters receptor activation and changes epitope exposure for antibody (Ab) recognition. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), a glycophosphoinositol-anchored protein, is a heavily glycosylated tumor antigen. However, the clinical significance and biological effect of CEACAM6 glycosylation has not been addressed in cancers...
September 11, 2017: Oncogene
https://www.readbyqxmd.com/read/28887103/unusual-glycosylation-of-proteins-beyond-the-universal-sequon-and-other-amino-acids
#10
REVIEW
Devawati Dutta, Chhabinath Mandal, Chitra Mandal
BACKGROUND: Glycosylation of proteins is the most common, multifaceted co- and post-translational modification responsible for many biological processes and cellular functions. Significant alterations and aberrations of these processes are related to various pathological conditions, and often turn out to be disease biomarkers. Conventional N-glycosylation occurs through the recognition of the consensus sequon, asparagine (Asn)-X-serine (Ser)/threonine (Thr), where X is any amino acid except for proline, with N-acetylglucosamine (GlcNAc) as the first glycosidic linkage...
September 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28880909/in-vivo-biological-activity-and-glycosylation-analysis-of-a-biosimilar-recombinant-human-follicle-stimulating-hormone-product-bemfola-compared-with-its-reference-medicinal-product-gonal-f
#11
Renato Mastrangeli, Abhijeet Satwekar, Francesca Cutillo, Cinzia Ciampolillo, Wolf Palinsky, Salvatore Longobardi
Recombinant human follicle-stimulating hormone (r-hFSH) is widely used in fertility treatment. Although biosimilar versions of r-hFSH (follitropin alfa) are currently on the market, given their structural complexity and manufacturing process, it is important to thoroughly evaluate them in comparison with the reference product. This evaluation should focus on how they differ (e.g., active component molecular characteristics, impurities and potency), as this could be associated with clinical outcome. This study compared the site-specific glycosylation profile and batch-to-batch variability of the in-vivo bioactivity of Bemfola, a biosimilar follitropin alfa, with its reference medicinal product GONAL-f...
2017: PloS One
https://www.readbyqxmd.com/read/28880465/profiling-novel-metabolic-biomarkers-for-parkinson-s-disease-using-in-depth-metabolomic-analysis
#12
Wei Han, Shraddha Sapkota, Richard Camicioli, Roger A Dixon, Liang Li
OBJECTIVE: To profile the amine/phenol submetabolome to determine potential metabolite biomarkers associated with Parkinson's disease (PD) and PD with incipient dementia. METHODS: At baseline of a 3-wave (18-month intervals) longitudinal study, serum samples were collected from 42 healthy controls and 43 PD patients. By wave 3 (year 3), 16 PD patients were diagnosed with dementia and were classified as PD with incipient dementia at baseline. Metabolomic profiling using dansylation isotope labeling liquid chromatography mass spectrometry was conducted to compare controls with the full PD, PD with no dementia, and PD with incipient dementia groups...
September 7, 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/28877926/effectiveness-of-quality-improvement-strategies-for-the-management-of-ckd-a-meta-analysis
#13
Samuel A Silver, Chaim M Bell, Glenn M Chertow, Prakesh S Shah, Kaveh Shojania, Ron Wald, Ziv Harel
BACKGROUND AND OBJECTIVES: Quality improvement interventions have enhanced care for other chronic illnesses, but their effectiveness for patients with CKD is unknown. We sought to determine the effects of quality improvement strategies on clinical outcomes in adult patients with nondialysis-requiring CKD. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We conducted a systematic review of randomized trials, searching Medline and the Cochrane Effective Practice and Organization of Care database from January of 2003 to April of 2015...
September 6, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28874509/the-maize-imprinted-gene-floury3-encodes-a-platz-protein-required-for-trna-and-5s-rrna-transcription-through-interaction-with-rna-polymerase-iii
#14
Qi Li, Jiechen Wang, Jianwei Ye, Xixi Zheng, Xiaoli Xiang, Changsheng Li, Miaomiao Fu, Qiong Wang, Zhi-Yong Zhang, Yongrui Wu
Maize (Zea mays) floury3 (fl3) is a classic semi-dominant negative mutant that exhibits severe defects in the endosperm but fl3 plants otherwise appear normal. We cloned the fl3 gene and determined that it encodes a PLATZ (plant AT-rich sequence- and zinc-binding) protein. The mutation in fl3 resulted in an Asn to His replacement in the conserved PLATZ domain, creating a dominant allele. Fl3 is specifically expressed in starchy endosperm cells and regulated by genomic imprinting, which leads to the suppressed expression of fl3 when transmitted through the male, perhaps as a consequence the semi-dominant behavior...
September 5, 2017: Plant Cell
https://www.readbyqxmd.com/read/28873704/fractionation-and-identification-of-antioxidant-peptides-from-an-enzymatically-hydrolysed-palmaria-palmata-protein-isolate
#15
Pádraigín A Harnedy, Martina B O'Keeffe, Richard J FitzGerald
Proteins derived from the macroalgal species Palmaria palmata have emerged as potential substrates for the generation of bioactive peptides. The aim of this study was to fractionate, identify and characterize antioxidant peptides from a P. palmata protein hydrolysate. The P. palmata protein hydrolysate generated with the food-grade proteolytic enzyme Corolase PP was sequentially fractionated using solid phase extraction and semi-preparative (SP) RP-HPLC. The most active SP-RP-HPLC peptide fraction (SP-RP-HPLC-30-F26) was analysed by ESI-MS/MS...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28870327/the-5-azoniaspiro-4-4-nonyl-group-for-improved-ms-peptide-analysis-a-novel-non-fragmenting-ionization-tag-for-mass-spectrometric-sensitive-sequencing-of-peptides
#16
Bartosz Setner, Magdalena Rudowska, Alicja Kluczyk, Piotr Stefanowicz, Zbigniew Szewczuk
A novel class of ionization tags, based on 5-azoniaspiro[4.4]nonyl (ASN(+)) scaffold were designed for improved analysis of peptides by electrospray tandem mass spectrometry (ESI-MS/MS). A new labeling agent, 1-{[3-oxo-3-(pentafluorophenoxy)propyl]carbamoyl}-5-azoniaspiro[4.4]nonane, was developed to react with amine and/or thiol group-containing peptides. The ionization efficiency of peptides resulting from derivatization was enhanced 10-100 fold, depending on the peptide sequence and hydrophobicity of the ionization tag...
September 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28860749/in-vitro-and-in-vivo-evaluation-of-novel-ngr-modified-liposomes-containing-brucine
#17
Shu Li, Xi-Peng Wang
In this study, a novel NGR (Asn-Gly-Arg) peptide-modified liposomal brucine was prepared by using spray-drying method. The surface morphology of the liposomes, encapsulation efficiency and particle size were investigated. The data showed that the addition of NGR did not produce any significant influence on brucine liposomes in terms of particle size or zeta potential. In addition, after 3 months of storage, no dramatic change such as visible aggregation, drug content changes or precipitation in the appearance of NGR-brucine liposomes occurred...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28860505/endolysin-lysef-p10-shows-potential-as-an-alternative-treatment-strategy-for-multidrug-resistant-enterococcus-faecalis-infections
#18
Mengjun Cheng, Yufeng Zhang, Xinwei Li, Jiaming Liang, Liyuan Hu, Pengjuan Gong, Lei Zhang, Ruopeng Cai, Hao Zhang, Jinli Ge, Yalu Ji, Zhimin Guo, Xin Feng, Changjiang Sun, Yongjun Yang, Liancheng Lei, Wenyu Han, Jingmin Gu
Phage-derived lysins can hydrolyse bacterial cell walls and show great potential for combating Gram-positive pathogens. In this study, the potential of LysEF-P10, a new lysin derived from a isolated Enterococcus faecalis phage EF-P10, as an alternative treatment for multidrug-resistant E. faecalis infections, was studied. LysEF-P10 shares only 61% amino acid identity with its closest homologues. Four proteins were expressed: LysEF-P10, the cysteine, histidine-dependent amidohydrolase/peptidase (CHAP) domain (LysEF-P10C), the putative binding domain (LysEF-P10B), and a fusion recombination protein (LysEF-P10B-green fluorescent protein)...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28858643/exploring-putative-inhibitors-of-death-associated-protein-kinase-1-dapk1-via-targeting-gly-glu-leu-gel-and-pro-glu-asn-pen-substrate-recognition-motifs
#19
Pratibha Singh, Priti Talwar
Recently, a new signaling complex Death Associated Protein Kinase 1 (DAPK1) ̶ N-methyl-D-aspartate receptor subtype 2B (NMDAR2B or NR2B) engaged in the neuronal death cascade was identified and it was found that after stroke injury, N-methyl-D-aspartate glutamate (NMDA) receptors interact with DAPK1 through NR2B subunit and lead to excitotoxicity via over-activation of NMDA receptors. An acute brain injury, such as stroke, is a serious life-threatening medical condition which occurs due to poor blood supply to the brain and further leads to neuronal cell death...
August 18, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28852803/the-hypertrehalosemic-neuropeptides-of-cicadas-are-structural-isomers-evidence-by-ion-mobility-mass-spectrometry
#20
Simone König, Heather Marco, Gerd Gäde
It has been known for more than 20 years that the neurosecretory glands of the cicadas, the corpora cardiaca, synthesize two isobaric peptides with hypertrehalosemic activity. Both decapeptides have exactly the same amino acid sequence (pGlu-Val-Asn-Phe-Ser-Pro-Ser-Trp-Gly-Asn-NH2) and mass but differ in their retention time in reversed-phase liquid chromatography. A synthetic peptide with the same sequence elutes together with the second more hydrophobic peptide peak of the natural cicada extract. It is not clear what modification is causing the described observations...
August 29, 2017: Analytical and Bioanalytical Chemistry
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