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https://www.readbyqxmd.com/read/28225562/the-adipokinetic-hormone-of-mantodea-in-comparison-to-other-dictyoptera
#1
Gerd Gäde, Heather G Marco
Six species of the order Mantodea (praying mantises) are investigated for the presence and sequence of putative adipokinetic hormones (AKHs). The selected species span a wide evolutionary range of various families and subfamilies of the clade Mantodea. The corpora cardiaca of the different species are dissected, methanolic extracts prepared, peptides separated by liquid chromatography, and AKHs detected and sequenced by ion trap mass spectrometry. All six species investigated contain an octapeptide with the primary structure pGlu-Val-Asn-Phe-Thr-Pro-Asn-Trp amide, which is code-named Emppe-AKH and had been found earlier in three other species of Mantodea...
February 22, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28220415/analysis-for-protein-glycosylation-of-pattern-recognition-receptors-in-plants
#2
Takaakira Inokuchi, Yusuke Saijo
Recognition of molecules typical of microbes or aberrant cellular states, termed microbe- or danger-associated molecular patterns (MAMPs/DAMPs), respectively, provides an important step in plant and animal innate immunity. In plants, pattern recognition receptors (PRRs) identified to date are limited to membrane-associated proteins, of which the majority has an extracellular leucine-rich repeat (LRR) or lysine-motif (LysM) domain. These PRRs undergo quality control (QC) in the Endoplasmic Reticulum (ER) that is dependent on Asn (N)-linked glycosylation (Glc3Man9GlcNAc2 conjugation) of their extracellular domain...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28216000/pharmacological-and-molecular-characterization-of-the-positive-allosteric-modulators-of-metabotropic-glutamate-receptor-2
#3
REVIEW
L Lundström, C Bissantz, J Beck, M Dellenbach, T J Woltering, J Wichmann, S Gatti
The metabotropic glutamate receptor 2 (mGlu2) plays an important role in the presynaptic control of glutamate release and several mGlu2 positive allosteric modulators (PAMs) have been under assessment for their potential as antipsychotics. The binding mode of mGlu2 PAMs is better characterized in functional terms while few data are available on the relationship between allosteric and orthosteric binding sites. Pharmacological studies characterizing binding and effects of two different chemical series of mGlu2 PAMs are therefore carried out here using the radiolabeled mGlu2 agonist (3)[H]-LY354740 and mGlu2 PAM (3)[H]-2,2,2-TEMPS...
February 16, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28215920/antimicrobial-drug-resistance-and-genetic-properties-of-salmonella-enterica-serotype-enteritidis-circulating-in-chicken-farms-in-tunisia
#4
Rakia Ben Salem, Mohamed S Abbassi, Vanesa García, Raquel García-Fierro, Javier Fernández, Hajer Kilani, Imen Jaouani, Monia Khayeche, Lilia Messadi, María Rosario Rodicio
This study focused on 77 isolates of Salmonella enterica serotype Enteritidis collected during 2009 to 2013 from healthy and sick chickens and environmental farm samples in Tunisia. Resistance to 14 antimicrobials and the encoding genes were analyzed. 66, 26, 6.5, 3.9 and 1.3% were pan-susceptible or showed resistance to nalidixic acid (Asp87 to Tyr and Asp87 to Asn substitutions in GyrA), ampicillin (blaTEM-1-like and blaSHV), sulfonamides (sul1and sul3) and streptomycin (strB), respectively. A single isolate with intermediate susceptibility to ciprofloxacin was positive for qnrB, whereas qnrA, qnrS or aac(6')-Ib-cr were not detected...
February 16, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28212316/succinimide-formation-from-an-ngr-containing-cyclic-peptide-computational-evidence-for-catalytic-roles-of-phosphate-buffer-and-the-arginine-side-chain
#5
Ryota Kirikoshi, Noriyoshi Manabe, Ohgi Takahashi
The Asn-Gly-Arg (NGR) motif and its deamidation product isoAsp-Gly-Arg (isoDGR) have recently attracted considerable attention as tumor-targeting ligands. Because an NGR-containing peptide and the corresponding isoDGR-containing peptide target different receptors, the spontaneous NGR deamidation can be used in dual targeting strategies. It is well known that the Asn deamidation proceeds via a succinimide derivative. In the present study, we computationally investigated the mechanism of succinimide formation from a cyclic peptide, c[CH₂CO-NGRC]-NH₂, which has recently been shown to undergo rapid deamidation in a phosphate buffer...
February 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28209512/n-glycan-dependent-cell-surface-expression-of-the-p2y2-receptor-and-n-glycan-independent-distribution-to-lipid-rafts
#6
Tetsuto Nakagawa, Chihiro Takahashi, Hitomi Matsuzaki, Shohei Takeyama, Shinpei Sato, Ayaka Sato, Yoshiyuki Kuroda, Hideyoshi Higashi
P2Y2 receptor (P2Y2R) is a G-protein-coupled receptor (GPCR) that couples with Gαq/11 and is stimulated by ATP and UTP. P2Y2R is involved in pain, proinflammatory changes, and blood pressure control. Some GPCRs are localized in lipid rafts for interaction with other signaling molecules. In this study, we prepared N-glycan-deficient mutants by mutating the two consensus Asn residues for N-glycosylation to Gln to examine intracellular localization and association with lipid rafts. Western blotting of the wild type (WT) protein and mutants (N9Q, N13Q, N9Q/N13Q) in COS-7 cells showed that both Asn residues were glycosylated in the WT...
February 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28206997/pulmonary-hypertension-in-preterm-infants-results-of-a-prospective-screening-program
#7
C G Weismann, J D Asnes, A Bazzy-Asaad, C Tolomeo, R A Ehrenkranz, M J Bizzarro
OBJECTIVE: Determine prevalence and associations with pulmonary hypertension (PH) in preterm infants. STUDY DESIGN: Prospective institutional echocardiographic PH screening at 36 to 38 weeks' corrected gestational age (GA) for infants born <32 weeks' GA who had bronchopulmonary dysplasia (BPD; group BPD), and infants without BPD who had a birth weight (BW) <750 g, or clinical suspicion for PH (group NoBPD). RESULTS: Two hundred and four infants were screened (GA 25...
February 16, 2017: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/28202898/engineering-a-lys-asn-isopeptide-bond-into-an-immunoglobulin-like-protein-domain-enhances-its-stability
#8
Hanna Kwon, Paul G Young, Christopher J Squire, Edward N Baker
The overall stability of globular protein structures is marginal, a balance between large numbers of stabilizing non-covalent interactions and a destabilizing entropic term. Higher stability can be engineered by introduction of disulfide bonds, provided the redox environment is controlled. The discovery of stabilizing isopeptide bond crosslinks, formed spontaneously between lysine and asparagine (or aspartic acid) side chains in certain bacterial cell-surface proteins suggests that such bonds could be introduced by protein engineering as an alternative protein stabilization strategy...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197753/asparaginase-pharmacology-challenges-still-to-be-faced
#9
REVIEW
Claudia Lanvers-Kaminsky
PURPOSE: The benefits of asparaginase (ASNASE) in the treatment of ALL and NHL are indisputable and new ASNASE preparations are under clinical development to overcome limitations of the actual ASNASE therapy, especially immunogenicity. Apart from ALL and NHL further indications of ASNASE are preclinically and clinically evaluated. METHODS: We reviewed ASNASE literature and especially focused on the mechanism of action, on biomarker, which determine ASNASE sensitivity and resistance, and on ASNASE pharmacodynamics in vivo...
February 14, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28195275/bond-strengths-of-orthodontic-metal-brackets-to-tribochemically-silica-coated-zirconia-surfaces-using-different-10-methacryloyloxydecyl-dihydrogen-phosphate-containing-primers
#10
Na-Hyun Kim, Yoon-Ji Kim, Dong-Yul Lee
PURPOSE: To evaluate the effects of tribochemical silica coating and different 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-containing primers on the shear bond strength (SBS) of orthodontic metal brackets to yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) surfaces. MATERIALS AND METHODS: One hundred ninety polished Y-TZP specimens were randomly assigned to 19 groups (n = 10): 30 specimens were used for surface analyses after polishing with 600-grit silicon carbide paper, airborneparticle abrasion with 50-μm alumina (A), or tribochemical silica coating (CoJet [C]); 160 specimens were used in SBS testing of orthodontic metal brackets to Y-TZP after alumina airborne-particle abrasion or tribochemical silica coating and application of either ESPE-Sil (S) (ASn, ASa, CSn, CSa), Alloy Primer (AP) (AAPn, AAPa, CAPn, CAPa), Clearfil Ceramic Primer (CP) (ACPn, ACPa, CCPn, CCPa), or Scotchbond Universal (U) (AUn, AUa, CUn, CUa) and either stored in water for 24 h (non-aged, n) or thermocycled 5000 times (aged, a)...
February 10, 2017: Journal of Adhesive Dentistry
https://www.readbyqxmd.com/read/28194973/implications-for-the-light-driven-chloride-ion-transport-mechanism-of-nonlabens-marinus-rhodopsin-3-by-its-photochemical-characteristics
#11
Takashi Tsukamoto, Susumu Yoshizawa, Takashi Kikukawa, Makoto Demura, Yuki Sudo
Several new retinal-based photoreceptor proteins that act as light-driven electrogenic halide ion pumps have recently been discovered. Some of them, called "NTQ" rhodopsins, contain a conserved Asn-Thr-Gln motif in the 3(rd) or C-helix. In this study, we investigated the photochemical characteristics of a NTQ rhodopsin, Nonlabens marinus rhodopsin 3 (NM-R3), which was discovered in the N. marinus S1-08(T) strain, using static and time-resolved spectroscopic techniques. We demonstrate that NM-R3 binds a Cl(-) in the vicinity of the retinal chromophore accompanied by a spectral blue shift from 568 nm in the absence of Cl(-) to 534 nm in the presence of Cl(-)...
February 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28193745/clarithromycin-based-triple-therapy-is-still-useful-as-an-initial-treatment-for-helicobacter-pylori-infection-in-the-dominican-republic
#12
Muhammad Miftahussurur, Modesto Cruz, Phawinee Subsomwong, José A Jiménez Abreu, Celso Hosking, Hiroyuki Nagashima, Junko Akada, Yoshio Yamaoka
Helicobacter pylori antibiotic susceptibility in the Dominican Republic has not been monitored. We assessed Hpylori antibiotic susceptibility in the Dominican Republic, and analyzed Hpylori mutations associated with antibiotic resistance. We recruited 158 dyspeptic patients in Santo Domingo and used agar dilution to test susceptibility to five antibiotics. Polymerase chain reaction-based sequencing was used to assess gyrA, gyrB, rdxA, frxA, and 23S rRNA mutations; next-generation sequencing was used to identify other metronidazole resistance-associated genes...
February 13, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28187204/differentiating-founder-and-chronic-hiv-envelope-sequences
#13
John M Murray, Stephen Maher, Talia Mota, Kazuo Suzuki, Anthony D Kelleher, Rob J Center, Damian Purcell
Significant progress has been made in characterizing broadly neutralizing antibodies against the HIV envelope glycoprotein Env, but an effective vaccine has proven elusive. Vaccine development would be facilitated if common features of early founder virus required for transmission could be identified. Here we employ a combination of bioinformatic and operations research methods to determine the most prevalent features that distinguish 78 subtype B and 55 subtype C founder Env sequences from an equal number of chronic sequences...
2017: PloS One
https://www.readbyqxmd.com/read/28179475/asparagine-linked-glycans-of-cryptosporidium-parvum-contain-a-single-long-arm-are-barely-processed-in-the-er-or-golgi-and-show-a-strong-bias-for-sites-with-threonine
#14
John R Haserick, Deborah R Leon, John Samuelson, Catherine E Costello
Cryptosporidium parvum causes severe diarrhea in infants in developing countries and in immunosuppressed persons, including those with AIDS. We are interested in the Asn-linked glycans (N-glycans) of C. parvum, because 1) the N-glycan precursor is predicted to contain five mannose and two glucose residues on a single long arm versus nine mannose and three glucose residues on the three-armed structure common in host N-glycans, 2) C. parvum is a rare eukaryote that lacks the machinery for N-glycan-dependent quality control of protein folding in the lumen of the Endoplasmic Reticulum (ER), and 3) ER and Golgi mannosidases, as well as glycosyltransferases that build complex N-glycans, are absent from the predicted proteome...
February 8, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28179079/exploiting-charge-differences-for-the-analysis-of-challenging-post-translational-modifications-by-capillary-electrophoresis-mass-spectrometry
#15
Klaus Faserl, Bettina Sarg, Verena Maurer, Herbert H Lindner
Reversed-phase high-performance liquid chromatography (RP-HPLC) in combination with mass spectrometry (MS) is typically employed for mapping modifications in proteins and peptides. Here we applied a low-flow capillary electrophoresis (CE) -electrospray ionization interface coupled to Orbitrap mass spectrometers to analyze challenging modifications such as asparagine deamidation, aspartate isomerization, arginine citrullination, and phosphopeptide isomers. We achieved excellent resolution of asparagine (Asn), aspartic acid (Asp) and isoaspartic acid (iso-Asp) containing peptides using a synthetic peptide mixture...
February 1, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28177776/characterization-of-a-salmonella-sugar-kinase-essential-for-utilization-of-fructose-asparagine
#16
Pradip K Biswas, Edward J Behrman, Venkat Gopalan
Salmonella can utilize fructose-asparagine (F-Asn), a naturally occurring Amadori product, as its sole carbon and nitrogen source. Conversion of F-Asn to the common intermediates glucose-6-phosphate, aspartate, and ammonia was predicted to involve the sequential action of an asparaginase, a kinase and a deglycase. Mutants lacking the deglycase are highly attenuated in mouse models of intestinal inflammation due to the toxic build-up of the deglycase substrate. The limited distribution of this metabolic pathway in the animal gut microbiome raises the prospects for antibacterial discovery...
August 10, 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177315/crystal-structure-of-the-n-terminal-anticodon-binding-domain-of-the-nondiscriminating-aspartyl-trna-synthetase-from-helicobacter-pylori
#17
Chomphunuch Songsiriritthigul, Suwimon Suebka, Chun Jung Chen, Pitchayada Fuengfuloy, Pitak Chuawong
The N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase (ND-AspRS) plays a crucial role in the recognition of both tRNA(Asp) and tRNA(Asn). Here, the first X-ray crystal structure of the N-terminal domain of this enzyme (ND-AspRS1-104) from the human-pathogenic bacterium Helicobacter pylori is reported at 2.0 Å resolution. The apo form of H. pylori ND-AspRS1-104 shares high structural similarity with the N-terminal anticodon-binding domains of the discriminating aspartyl-tRNA synthetase (D-AspRS) from Escherichia coli and ND-AspRS from Pseudomonas aeruginosa, allowing recognition elements to be proposed for tRNA(Asp) and tRNA(Asn)...
February 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28165720/intein-promoted-cyclization-of-aspartic-acid-flanking-the-intein-leads-to-atypical-n-terminal-cleavage
#18
Christopher J Minteer, Nicolle M Siegart, Kathryn M Colelli, Xinyue Liu, Robert J Linhardt, Chunyu Wang, Alvin V Gomez, Julie N Reitter, Kenneth V Mills
Protein splicing is a post-translational reaction facilitated by an intein, or intervening protein, which involves the removal of the intein and the ligation of the flanking polypeptides, or exteins. A DNA polymerase II intein from Pyrococcus abyssi (Pab PolII intein) can promote protein splicing in vitro on incubation at high temperature. Mutation of active site residues Cys1, Gln185, and Cys+1 to Ala results in an inactive intein precursor, which cannot promote the steps of splicing, including cleavage of the peptide bond linking the N-extein and intein (N-terminal cleavage)...
February 15, 2017: Biochemistry
https://www.readbyqxmd.com/read/28154982/how-the-extra-methylene-group-affects-the-ligation-properties-of-glu-vs-asp-and-gln-vs-asn-amino-acids-a-dft-pcm-study
#19
Todor Dudev, Lyudmila Doudeva
The effect of the extra methylene group on the ligation properties of glutamic (Glu) vs. aspartic (Asp) acid, and glutamine (Gln) vs. asparagine (Asn) amino acids-two pairs of protein building blocks differing by the length of their side chains-has been studied by employing DFT calculations combined with polarizable continuum model (PCM) computations. Complexes of the nominal species with partner ligands of various structures, charge states, and degree of solvent exposure have been examined. The results obtained reveal that the difference in the alkyl chain length of these amino acid residues does not affect the mode of their binding...
February 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28150507/the-microinr-portable-coagulometer-analytical-quality-and-user-friendliness-of-a-pt-inr-point-of-care-instrument
#20
Pia Bükmann Larsen, Elin Storjord, Åsne Bakke, Tone Bukve, Mikael Christensen, Joakim Eikeland, Vegar Engeland Haugen, Kristin Husby, Rie McGrail, Solveig Meier Mikaelsen, Grete Monsen, Mette Fogh Møller, Jan Nybo, Jesper Revsholm, Aslaug Johanne Risøy, Unni Marie Skålsvik, Heidi Strand, Reyes Serrano Teruel, Elvar Theodorsson
Regular measurement of prothrombin time as an international normalized ratio PT (INR) is mandatory for optimal and safe use of warfarin. Scandinavian evaluation of laboratory equipment for primary health care (SKUP) evaluated the microINR portable coagulometer (microINR(®)) (iLine Microsystems S.L., Spain) for measurement of PT (INR). Analytical quality and user-friendliness were evaluated under optimal conditions at an accredited hospital laboratory and at two primary health care centres (PHCCs). Patients were recruited at the outpatient clinic of the Laboratory of Medical Biochemistry, St Olav's University Hospital, Trondheim, Norway (n = 98) and from two PHCCs (n = 88)...
April 2017: Scandinavian Journal of Clinical and Laboratory Investigation
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