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https://www.readbyqxmd.com/read/28711240/characterization-and-bioactivities-of-the-exopolysaccharide-from-a-probiotic-strain-of-lactobacillus-plantarum-wlpl04
#1
Zhengqi Liu, Zhihong Zhang, Liang Qiu, Fen Zhang, Xiongpeng Xu, Hua Wei, Xueying Tao
Exopolysaccharide (EPS) was extracted and purified from Lactobacillus plantarum WLPL04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. It has a molecular weight of 6.61 × 10(4) Da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. Microstructural studies demonstrated that the EPS appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. The preliminary in vitro assays indicated that the EPS could significantly inhibit the adhesion of Escherichia coli O157:H7 to HT-29 cells in competition, replacement, and inhibition assays at a dose of 1...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28691662/role-of-the-inner-membrane-histidine-kinase-rcsc-and-outer-membrane-lipoprotein-rcsf-in-the-activation-of-the-rcs-phosphorelay-signal-transduction-system-in-escherichia-coli
#2
Takatsugu Sato, Akira Takano, Nanako Hori, Tomoko Izawa, Takayoshi Eda, Kota Sato, Mitsuru Umekawa, Hiroyoshi Miyagawa, Kenji Matsumoto, Ayako Muramatsu-Fujishiro, Kouji Matsumoto, Satoshi Matsuoka, Hiroshi Hara
The Rcs phosphorelay signal transduction system of Escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. The outer-membrane lipoprotein RcsF is an essential component of the Rcs system. Mislocalization of RcsF to the periplasm or the cytoplasmic membrane leads to high activation of the Rcs system, suggesting that RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system. This is consistent with the result reported by Cho et al...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28652301/stand-alone-eal-domain-proteins-form-a-distinct-subclass-of-eal-proteins-involved-in-regulation-of-cell-motility-and-biofilm-formation-in-enterobacteria
#3
Youssef El Mouali, Hyunhee Kim, Irfan Ahmad, Annelie Brauner, Ying Liu, Mikael Skurnik, Michael Y Galperin, Ute Römling
The second messenger cyclic di-GMP is almost ubiquitous among bacteria and so are the cyclic di-GMP turnover proteins, which mediate the transition between motility and sessility. EAL domain proteins have been characterized as cyclic di-GMP-specific phosphodiesterases. While most EAL domain proteins contain additional, usually N-terminal, domains, there is a distinct family of proteins within stand-alone EAL domains, exemplified by Salmonella enterica serovar Typhimurium proteins STM3611 (YhjH/PdeH), a c-di-GMP-specific phosphodiesterase, and the enzymatically inactive STM1344 (YdiV/CdgR) and STM1697, which regulate bacterial motility through interaction with the flagella master regulator FlhDC...
June 26, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28644912/biofilm-formation-and-disinfectant-resistance-of-salmonella-spp-in-mono-and-dual-species-with-pseudomonas-aeruginosa
#4
Xinyi Pang, Yishan Yang, Hyun-Gyun Yuk
AIMS: This study aimed to evaluate the biofilm formation and disinfectant resistance of Salmonella cells in mono- and dual-species biofilms with Pseudomonas aeruginosa, and to investigate the role of extracellular polymeric substances (EPS) in protection of biofilms against disinfection treatment. METHODS AND RESULTS: The populations of Salmonella in mono- or dual-species biofilms with P. aeruginosa on stainless steel (SS) coupons were determined before and after exposure to commercial disinfectant, 50 μg ml(-1) chlorine or 200 μg ml(-1) Ecolab(®) Whisper(™) V (a blend of four effective quaternary ammonium compounds)...
June 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28635589/regulation-of-escherichia-coli-pathogenesis-by-alternative-sigma-factor-n
#5
James T Riordan, Avishek Mitra
σ(N) (also σ(54)) is an alternative sigma factor subunit of the RNA polymerase complex that regulates the expression of genes from many different ontological groups. It is broadly conserved in the Eubacteria with major roles in nitrogen metabolism, membrane biogenesis, and motility. σ(N) is encoded as the first gene of a five-gene operon including rpoN (σ(N)), ptsN, hpf, rapZ, and npr that has been genetically retained among species of Escherichia, Shigella, and Salmonella. In an increasing number of bacteria, σ(N) has been implicated in the control of genes essential to pathogenic behavior, including those involved in adherence, secretion, immune subversion, biofilm formation, toxin production, and resistance to both antimicrobials and biological stressors...
June 2017: EcoSal Plus
https://www.readbyqxmd.com/read/28611217/rna-target-profiles-direct-the-discovery-of-virulence-functions-for-the-cold-shock-proteins-cspc-and-cspe
#6
Charlotte Michaux, Erik Holmqvist, Erin Vasicek, Malvika Sharan, Lars Barquist, Alexander J Westermann, John S Gunn, Jörg Vogel
The functions of many bacterial RNA-binding proteins remain obscure because of a lack of knowledge of their cellular ligands. Although well-studied cold-shock protein A (CspA) family members are induced and function at low temperature, others are highly expressed in infection-relevant conditions. Here, we have profiled transcripts bound in vivo by the CspA family members of Salmonella enterica serovar Typhimurium to link the constitutively expressed CspC and CspE proteins with virulence pathways. Phenotypic assays in vitro demonstrated a crucial role for these proteins in membrane stress, motility, and biofilm formation...
June 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28607157/anaerobic-cysteine-degradation-and-potential-metabolic-coordination-in-salmonella-enterica-and-escherichia-coli
#7
Melissa Loddeke, Barbara Schneider, Tamiko Oguri, Iti Mehta, Zhenyu Xuan, Larry Reitzer
Salmonella enterica has two CutR-activated enzymes that degrade cysteine: the aerobic CdsH and an unidentified anaerobic enzyme; E. coli only has the latter. To identify the anaerobic enzyme, transcript profiling was performed from E. coli without cutR and with overexpressed cutR 37 genes showed at least a five-fold change in expression, and the cutPA (formerly yhaOM) operon had the greatest differential. Homology suggested that CutP and CutA are a cysteine transporter and an iron-sulfur-containing cysteine desulfidase, respectively...
June 12, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28605289/effect-of-sub-lethal-concentrations-of-biocides-on-the-structural-parameters-and-viability-of-the-biofilms-formed-by-salmonella-typhimurium
#8
Rosa Capita, Laura Buzón-Durán, Félix Riesco-Peláez, Carlos Alonso-Calleja
This study aimed to investigate the effect of sub-minimum inhibitory concentrations (sub-MICs) of three food-grade biocides (benzalkonium chloride -BZK-, trisodium phosphate -TSP-, and sodium hypochlorite -SHY-) on Salmonella biofilms. The structural parameters and bacterial viability of the biofilms formed by a S. Typhimurium isolate from poultry was investigated by means of confocal laser scanning microscopy after staining with SYTO9 and propidium iodide. The MIC values for Salmonella cells before exposure to subinhibitory concentrations of biocides were 8...
June 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28603807/bdm-mediated-regulation-of-flagellar-biogenesis-in-escherichia-coli-and-salmonella-enterica-serovar-typhimurium
#9
Jaejin Lee, Dae-Jun Kim, Ji-Hyun Yeom, Kangseok Lee
Synthesis of the flagellar apparatus in Escherichia coli is mediated via complex regulatory pathways. A previous study indicated that the protein encoded by the biofilm-dependent modulation (bdm) gene is linked closely with a regulatory pathway for flagellar assembly. However, the specific role of Bdm in flagellar biogenesis remains unknown. Herein, we showed that Bdm interacts with FlgM and inhibits its function as an anti-σ28 factor, which induces the transcription of flagellar late-class genes in E. coli...
June 12, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28598348/mortality-from-selected-diseases-that-can-be-transmitted-by-water-united-states-2003-2009
#10
J W Gargano, E A Adam, S A Collier, K E Fullerton, S J Feinman, M J Beach
Diseases spread by water are caused by fecal-oral, contact, inhalation, or other routes, resulting in illnesses affecting multiple body systems. We selected 13 pathogens or syndromes implicated in waterborne disease outbreaks or other well-documented waterborne transmission (acute otitis externa, Campylobacter, Cryptosporidium, Escherichia coli (E. coli), free-living ameba, Giardia, Hepatitis A virus, Legionella (Legionnaires' disease), nontuberculous mycobacteria (NTM), Pseudomonas-related pneumonia or septicemia, Salmonella, Shigella, and Vibrio)...
June 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/28595324/bacteriophage-use-to-control-salmonella-biofilm-on-surfaces-present-in-chicken-slaughterhouses
#11
Keila Carolina de Ornellas Dutka Garcia, Isadora Mainieri de Oliveira Corrêa, Larissa Quinto Pereira, Tarcísio Macedo Silva, Mateus de Souza Ribeiro Mioni, Ana Carolina de Moraes Izidoro, Igor Henrique Vellano Bastos, Guilherme Augusto Marietto Gonçalves, Adriano Sakai Okamoto, Raphael Lucio Andreatti Filho
Foodborne diseases represent a major risk to public health worldwide. Pathogenic bacteria can live in the form of biofilm within the food industry, providing a permanent source of contamination. The aim of this study was to evaluate the influence of the types of adhesion surfaces on Salmonella biofilm formation at eight different times, and analyze the action time of a bacteriophage pool on established biofilms. Most of the samples used were classified as weak biofilm producers, with serovars Enteritidis and Heidelberg showing the highest frequency of biofilm formation...
June 8, 2017: Poultry Science
https://www.readbyqxmd.com/read/28593349/transcriptomic-analysis-of-the-swarm-motility-phenotype-of-salmonella-enterica-serovar-typhimurium-mutant-defective-in-periplasmic-glucan-synthesis
#12
Arvind A Bhagwat, Lynn Young, Allen D Smith, Medha Bhagwat
Movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. Salmonella enterica serovar Typhimurium mutants defective in Osmoregulated periplasmic glucans (OPG) synthesis are unable to exhibit motility on moist surfaces (swarming); however, their mobility in liquid (swim motility) remains unaffected. In order to understand the role of OPG in swarm motility, transcriptomic analysis was performed using cells growing on a moist agar surface...
June 8, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28588134/the-rcs-regulated-colanic-acid-capsule-maintains-membrane-potential-in-salmonella-enterica-serovar-typhimurium
#13
Jasmine M Pando, Joyce E Karlinsey, Jimmie C Lara, Stephen J Libby, Ferric C Fang
The Rcs phosphorelay and Psp (phage shock protein) systems are envelope stress responses that are highly conserved in gammaproteobacteria. The Rcs regulon was found to be strongly induced during metal deprivation of Salmonella enterica serovar Typhimurium lacking the Psp response. Nineteen genes activated by the RcsA-RcsB response regulator make up an operon responsible for the production of colanic acid capsular polysaccharide, which promotes biofilm development. Despite more than half a century of research, the physiological function of colanic acid has remained elusive...
June 6, 2017: MBio
https://www.readbyqxmd.com/read/28587762/two-novel-roles-of-buffalo-milk-lactoperoxidase-antibiofilm-agent-and-immunomodulator-against-multidrug-resistant-salmonella-enterica-serovar-typhi-and-listeria-monocytogenes
#14
Likaa Mahdi, Hadeel Musafer, Luma Zwain, Istabreq Salman, Ikbal Al-Joofy, Khetam Rasool, Amal Mussa, Sana'a Al-Kakei, Rasha Al-Oqaili, Shaymaa Al-Alak, Amal Chaloob, Afrah Abdulkareem, Bydaa Hussein, Nada Mahdi, Nehad Taher
The increasing occurrence of multidrug resistant bacteria causing bacteremia infection, constitutes a major health problem, difficult-to-treat bacteremia due to its ability to form biofilm. Buffalo milk lactoperoxidase (BMLpo) is effective and safe to use as bacteriostatic agent. The MIC of BMLpo and amikacin were used to evaluate the antibiofilm activity against resistant L. monocytogenes and S. typhi. Prophylactic effects of BMLpo against L. monocytogenes and S. typhi bacteremia in vivo have been tested and ELISA test used to evaluate serum cytokines...
June 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28561639/susceptibility-of-salmonella-biofilm-and-planktonic-bacteria-to-common-disinfectant-agents-used-in-poultry-processing
#15
Tereza Chylkova, Myrna Cadena, Aura Ferreiro, Maurice Pitesky
Poultry contaminated with Salmonella enterica subsp. enterica are a major cause of zoonotic foodborne gastroenteritis. Salmonella Heidelberg is a common serotype of Salmonella that has been implicated as a foodborne pathogen associated with the consumption of improperly prepared chicken. To better understand the effectiveness of common antimicrobial disinfectants (i.e., peroxyacetic acid [PAA], acidified hypochlorite [aCH], and cetylpyridinium chloride [CPC]), environmental isolates of nontyphoidal Salmonella were exposed to these agents under temperature, concentration, and contact time conditions consistent with poultry processing...
May 31, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28542593/lack-of-the-pga-exopolysaccharide-in-salmonella-as-an-adaptive-trait-for-survival-in-the-host
#16
Maite Echeverz, Begoña García, Amaia Sabalza, Jaione Valle, Toni Gabaldón, Cristina Solano, Iñigo Lasa
Many bacteria build biofilm matrices using a conserved exopolysaccharide named PGA or PNAG (poly-β-1,6-N-acetyl-D-glucosamine). Interestingly, while E. coli and other members of the family Enterobacteriaceae encode the pgaABCD operon responsible for PGA synthesis, Salmonella lacks it. The evolutionary force driving this difference remains to be determined. Here, we report that Salmonella lost the pgaABCD operon after the divergence of Salmonella and Citrobacter clades, and previous to the diversification of the currently sequenced Salmonella strains...
May 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28540585/antibiofilm-activity-and-mode-of-action-of-dmso-alone-and-its-combination-with-afatinib-against-gram-negative-pathogens
#17
Mohd Fakharul Zaman Raja Yahya, Zazali Alias, Saiful Anuar Karsani
Biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. Enclosed in a self-produced extracellular polymeric substance (EPS) matrix, the biofilms often cause persistent infections. The objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (DMSO) and afatinib against Gram-negative pathogens. Test microorganisms used in this study were Escherichia coli ATCC 1299, Pseudomonas aeruginosa ATCC 10145, and Salmonella typhimurium ATCC 14028...
May 24, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28532805/efficacy-of-disinfectants-and-detergents-intended-for-a-pig-farm-environment-where-salmonella-is-present
#18
Rebecca J Gosling, Ian Mawhinney, Kelly Vaughan, Robert H Davies, Richard P Smith
Disinfection is a useful component of disease control, although products and chemical groups vary in their activity against different pathogens. This study investigated the ability of fifteen disinfectants to eliminate pig-associated Salmonella. Active compounds of products included chlorocresol, glutaraldehyde/formaldehyde, glutaraldehyde/quaternary ammonium compounds (QAC), iodine, peracetic acid and potassium peroxomonosulphate. Six detergents were also tested for their ability to dislodge faecal material, and interactions with specific disinfectants...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28511110/determination-of-regional-relationships-among-salmonella-spp-isolated-from-retail-pork-circulating-in-the-chiang-mai-municipality-area-using-a-wgs-data-approach
#19
Prapas Patchanee, Thanaporn Eiamsam-Ang, Juntakarn Vanaseang, Phacharaporn Boonkhot, Pakpoom Tadee
Salmonella is recognized as a significant zoonotic foodborne pathogen, and pork products are involved in one-fifth of infections. Whole genome sequencing data of Salmonella isolated from retail's pork circulating in the Chiang Mai Municipality area between April 2013 and September 2014, were used to focus on genetic diversity and proven in pig-human transmission based on Multilocus Sequence Typing (MLST). Additionally, WGS data were used to investigate virulence genes, to assess the hazard or pathogenic potential transferred into the food production chain...
May 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28503171/persistence-of-salmonella-typhimurium-lt2-in-soil-enhanced-after-growth-in-lettuce-medium
#20
Eva Fornefeld, Jasper Schierstaedt, Sven Jechalke, Rita Grosch, Adam Schikora, Kornelia Smalla
The persistence of Salmonella in the environment is influenced by a multitude of biotic and abiotic factors. In addition, its persistence can be influenced by preadaptation before the introduction into the environment. In order to study how preadaptation changes the survival of Salmonella in soil and therefore its potential to colonize the phytosphere, we developed a new medium based on lettuce material [lettuce medium (LM)]. Salmonella enterica serovar Typhimurium strain LT2 was used as a model for Salmonella in this study...
2017: Frontiers in Microbiology
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