keyword
https://read.qxmd.com/read/38530280/oma1-protease-eliminates-arrested-protein-import-intermediates-upon-mitochondrial-depolarization
#21
JOURNAL ARTICLE
Magda Krakowczyk, Anna M Lenkiewicz, Tomasz Sitarz, Dominika Malinska, Mayra Borrero, Ben Hur Marins Mussulini, Vanessa Linke, Andrzej A Szczepankiewicz, Joanna M Biazik, Agata Wydrych, Hanna Nieznanska, Remigiusz A Serwa, Agnieszka Chacinska, Piotr Bragoszewski
Most mitochondrial proteins originate from the cytosol and require transport into the organelle. Such precursor proteins must be unfolded to pass through translocation channels in mitochondrial membranes. Misfolding of transported proteins can result in their arrest and translocation failure. Arrested proteins block further import, disturbing mitochondrial functions and cellular proteostasis. Cellular responses to translocation failure have been defined in yeast. We developed the cell line-based translocase clogging model to discover molecular mechanisms that resolve failed import events in humans...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38530233/effects-of-exogenous-application-of-meja-and-sa-on-the-physiological-and-molecular-response-of-dusa-avocado-to-rosellinia-necatrix
#22
JOURNAL ARTICLE
Ana Moreno-Pérez, Elsa Martínez-Ferri, Noëlani van den Berg, Clara Pliego
Methyl jasmonate (MeJA) and salicylic acid (SA) are important in mediating plant responses to abiotic and biotic stresses and can act as elicitors by triggering plant defense responses similar to those induced by pathogens and may even provide long-term protection against them. Thus, exogenous application of MeJA and SA could protect susceptible avocado plants against white root rot (WRR) disease caused by the necrotrophic fungus Rosellinia necatrix, one of the main diseases affecting avocado orchards. This work evaluates the effects of MeJA or SA on the physiological and molecular response of susceptible 'Dusa' avocado rootstock, and their ability to provide some protection against WRR...
March 26, 2024: Plant Disease
https://read.qxmd.com/read/38529845/predicting-anti-covid-19-potential-in-silico-analysis-of-mauritine-compound-from-ziziphus-spina-christi-as-a-promising-papain-like-protease-plpro-inhibitor
#23
JOURNAL ARTICLE
Taufik Muhammad Fakih, Fitrianti Darusman, Riry Apriliani, Syifa Prahayati, Dwi Syah Fitra Ramadhan, Aulia Fikri Hidayat, Aden Dhana Rizkita, Tegar Achsendo Yuniarta
The COVID-19 pandemic caused by the SARS-CoV-2 virus, recognized by the World Health Organization (WHO), has led to 164,523,894 confirmed cases and 3,412,032 deaths globally as of May 20, 2021. SARS-CoV-2 encodes crucial proteases for its replication cycle, including the papain-like protease (PLpro), presenting a potential target for developing COVID-19 treatments. Mauritine, a cyclopeptide alkaloid found in the Ziziphus-spina christi plant, exhibits antiviral properties and was investigated for its affinity and toxicity towards PLpro using molecular docking through MGLTools 1...
March 26, 2024: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/38529602/fragment-based-drug-discovery-strategy-and-its-application-to-the-design-of-sars-cov-2-main-protease-inhibitor
#24
JOURNAL ARTICLE
Yu Jiang, Yingnan Wu, Jing Wang, Yuheng Ma, Hui Yu, Zhanli Wang
Severe Acute Respiratory Syndrome Coronavirus Type 2 (SARS-CoV-2) emerged at the end of 2019, causing a highly infectious and pathogenic disease known as 2019 coronavirus disease. This disease poses a serious threat to human health and public safety. The SARS-CoV-2 main protease (Mpro) is a highly sought-after target for developing drugs against COVID-19 due to its exceptional specificity. Its crystal structure has been extensively documented. Numerous strategies have been employed in the investigation of Mpro inhibitors...
March 25, 2024: Current Medicinal Chemistry
https://read.qxmd.com/read/38529542/the-antiviral-potential-of-the-antiandrogen-enzalutamide-and-the-viral-androgen-signaling-interplay-in-seasonal-coronaviruses
#25
JOURNAL ARTICLE
Oluwadamilola D Ogunjinmi, Tukur Abdullahi, Riaz-Ali Somji, Charlotte L Bevan, Wendy S Barclay, Nigel Temperton, Greg N Brooke, Efstathios S Giotis
The sex disparity in COVID-19 outcomes with males generally faring worse than females has been associated with the androgen-regulated expression of the protease TMPRSS2 and the cell receptor ACE2 in the lung and fueled interest in antiandrogens as potential antivirals. In this study, we explored enzalutamide, an antiandrogen used commonly to treat prostate cancer, as a potential antiviral against the human coronaviruses which cause seasonal respiratory infections (HCoV-NL63, -229E, and -OC43). Using lentivirus-pseudotyped and authentic HCoV, we report that enzalutamide reduced 229E and NL63 entry and infection in both TMPRSS2- and nonexpressing immortalized cells, suggesting a TMPRSS2-independent mechanism...
March 2024: Journal of Medical Virology
https://read.qxmd.com/read/38529321/molecular-regulation-of-transforming-growth-factor-%C3%AE-1-induced-thioredoxin-interacting-protein-ubiquitination-and-proteasomal-degradation-in-lung-fibroblasts-implication-in-pulmonary-fibrosis
#26
JOURNAL ARTICLE
Sarah J Taleb, Qinmao Ye, Boina Baoyinna, Michael Dedad, Dakshin Pisini, Narasimham L Parinandi, Lewis C Cantley, Jing Zhao, Yutong Zhao
Thioredoxin-interacting protein (TXNIP) plays a critical role in regulation of cellular redox reactions and inflammatory responses by interacting with thioredoxin (TRX) or the inflammasome. The role of TXNIP in lung fibrosis and molecular regulation of its stability have not been well studied. Therefore, here we investigated the molecular regulation of TXNIP stability and its role in TGF-β1-mediated signaling in lung fibroblasts. TXNIP protein levels were significantly decreased in lung tissues from bleomycin-challenged mice...
March 2024: J Respir Biol Transl Med
https://read.qxmd.com/read/38528645/pcolce2-overexpression-promotes-supporting-cell-reprogramming-in-the-neonatal-mouse-cochlea
#27
JOURNAL ARTICLE
Changling Xu, Liyan Zhang, Yinyi Zhou, Haoliang Du, Jieyu Qi, Fangzhi Tan, Li Peng, Xingliang Gu, Nianci Li, Qiuhan Sun, Ziyu Zhang, Yicheng Lu, Xiaoyun Qian, Busheng Tong, Jiaqiang Sun, Renjie Chai, Yi Shi
Hair cell (HC) damage is a leading cause of sensorineural hearing loss, and in mammals supporting cells (SCs) are unable to divide and regenerate HCs after birth spontaneously. Procollagen C-endopeptidase enhancer 2 (Pcolce2), which encodes a glycoprotein that acts as a functional procollagen C protease enhancer, was screened as a candidate regulator of SC plasticity in our previous study. In the current study, we used adeno-associated virus (AAV)-ie (a newly developed adeno-associated virus that targets SCs) to overexpress Pcolce2 in SCs...
March 25, 2024: Cell Proliferation
https://read.qxmd.com/read/38528501/newly-discovered-clouting-interplay-between-matrix-metalloproteinases-structures-and-novel-quaternary-ammonium-k21-computational-and-in-vivo-testing
#28
JOURNAL ARTICLE
Ranjeet Ajit Bapat, Kit-Kay Mak, Mallikarjuna Rao Pichika, Jia Chern Pang, Seow Liang Lin, Suan Phaik Khoo, Umer Daood
AIMS AND OBJECTIVES: To analyze anti-MMP mode of action of Quaternary Ammonium Silane (QAS, codenamed as k21) by binding onto specific MMP site using computational molecular simulation and Anti-Sortase A (SrtA) mode of action by binding onto specific site using computational molecular simulation. MATERIALS AND METHODS: In silico Molecular Dynamics (MD) was used to determine the interactions of K21 inside the pocket of the targeted protein (crystal structure of fibroblast collagenase-1 complexed to a diphenyl-ether sulphone based hydroxamic acid; PDB ID: 966C; Crystal structure of MMP-2 active site mutant in complex with APP-derived decapeptide inhibitor...
March 25, 2024: BMC Oral Health
https://read.qxmd.com/read/38527904/an-essential-protease-ftsh-influences-daptomycin-resistance-acquisition-in-enterococcus-faecalis
#29
JOURNAL ARTICLE
Zeus Jaren Nair, Iris Hanxing Gao, Aslam Firras, Kelvin Kian Long Chong, Eric D Hill, Pei Yi Choo, Cristina Colomer-Winter, Qingyan Chen, Caroline Manzano, Kevin Pethe, Kimberly A Kline
Daptomycin is a last-line antibiotic commonly used to treat vancomycin-resistant Enterococci, but resistance evolves rapidly and further restricts already limited treatment options. While genetic determinants associated with clinical daptomycin resistance (DAPR ) have been described, information on factors affecting the speed of DAPR acquisition is limited. The multiple peptide resistance factor (MprF), a phosphatidylglycerol-modifying enzyme involved in cationic antimicrobial resistance, is linked to DAPR in pathogens such as methicillin-resistant Staphylococcus aureus...
March 25, 2024: Molecular Microbiology
https://read.qxmd.com/read/38527561/a-two-step-regulatory-circuit-involving-spo0a-abrb-activates-mersacidin-biosynthesis-in-bacillus-subtilis
#30
JOURNAL ARTICLE
Lars Lilge, Oscar P Kuipers
Due to intramolecular ring structures, the ribosomally produced and post-translationally modified peptide mersacidin shows antimicrobial properties comparable to those of vancomycin without exhibiting cross-resistance. Although the principles of mersacidin biosynthesis are known, there is no information on the molecular control processes for the initial stimulation of mersacidin bioproduction. By using Bacillus subtilis for heterologous biosynthesis, a considerable amount of mersacidin could be produced without the mersacidin-specific immune system and the mersacidin-activating secretory protease...
March 23, 2024: International Journal of Antimicrobial Agents
https://read.qxmd.com/read/38527414/effects-of-different-supplemental-levels-of-protease-de200-on-the-production-performance-egg-quality-and-cecum-microflora-of-laying-hens
#31
JOURNAL ARTICLE
Peiran Cai, Shiqi Liu, Yuang Tu, Dabo Fu, Wei Zhang, Xixi Zhang, Ying Zhou, Tizhong Shan
This study aimed to investigate the effects of different levels of the protease DE200TM on the performance, egg quality, organ index and cecum microflora of Hy-line W36 laying hens. In this experiment, a total of 180 laying hens aged 300 days were randomly divided into three treatment groups and fed diets containing 0, 100 or 200 g/t DE200. The experimental period was eight weeks, including two weeks of prefeeding and six weeks of the formal experiment. Regular feeding was performed thrice a day and eggs were collected twice daily, and the feed intake and the egg quality were recorded...
March 25, 2024: Journal of Animal Science
https://read.qxmd.com/read/38527094/deubiquitinating-activity-of-sars-cov-2-papain-like-protease-does-not-influence-virus-replication-or-innate-immune-responses-in-vivo
#32
JOURNAL ARTICLE
Mariska van Huizen, Jonna R Bloeme-Ter Horst, Heidi L M de Gruyter, Paul P Geurink, Gerbrand J van der Heden van Noort, Robert C M Knaap, Tessa Nelemans, Natacha S Ogando, Anouk A Leijs, Nadya Urakova, Brian L Mark, Eric J Snijder, Sebenzile K Myeni, Marjolein Kikkert
The coronavirus papain-like protease (PLpro) is crucial for viral replicase polyprotein processing. Additionally, PLpro can subvert host defense mechanisms by its deubiquitinating (DUB) and deISGylating activities. To elucidate the role of these activities during SARS-CoV-2 infection, we introduced mutations that disrupt binding of PLpro to ubiquitin or ISG15. We identified several mutations that strongly reduced DUB activity of PLpro, without affecting viral polyprotein processing. In contrast, mutations that abrogated deISGylating activity also hampered viral polyprotein processing and when introduced into the virus these mutants were not viable...
March 25, 2024: PLoS Pathogens
https://read.qxmd.com/read/38526868/the-ecotin-like-peptidase-inhibitor-of-trypanosoma-cruzi-prevents-tmprss2-par2-tlr4-crosstalk-downmodulating-infection-and-inflammation
#33
JOURNAL ARTICLE
Tatiana F R Costa, Carolina M C Catta-Preta, Amy Goundry, Danielle B Carvalho, Nathalia S Rodrigues, Aislan C Vivarini, Mayra Fonseca de Abreu, Flavia C G Reis, Ana Paula C A Lima
Trypanosoma cruzi is the causative agent of Chagas disease, a chronic pathology that affects the heart and/or digestive system. This parasite invades and multiplies in virtually all nucleated cells, using a variety of host cell receptors for infection. T. cruzi has a gene that encodes an ecotin-like inhibitor of serine peptidases, ISP2. We generated ISP2-null mutants (Δisp2) in T. cruzi Dm28c using CRISPR/Cas9. Epimastigotes of Δisp2 grew normally in vitro but were more susceptible to lysis by human serum compared to parental and ISP2 add-back lines...
March 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38526531/exploring-the-efficacy-of-noncovalent-sars-cov-2-main-protease-inhibitors-a-computational-study-incorporating-design-admet-molecular-dynamics-simulation-and-dft-analysis
#34
JOURNAL ARTICLE
Fei Xiong, Yan-Jun Zhang, Hui-Ying Jiang, Zhong-Hua Wang
The SARS-CoV-2 main protease, as a key target for antiviral therapeutics, is instrumental in maintaining virus stability, facilitating translation, and enabling the virus to evade innate immunity. Our research focused on designing non-covalent inhibitors to counteract the action of this protease. Utilizing a 3D-QSAR model and contour map, we successfully engineered eight novel non-covalent inhibitors. Further evaluation and comparison of these novel compounds through methodologies including molecular docking, ADMET analysis, frontier molecular orbital studies, molecular dynamics simulations, and binding free energy revealed that the inhibitors N02 and N03 demonstrated superior research performance (N02 ΔGbind = -206...
March 25, 2024: Chemistry & Biodiversity
https://read.qxmd.com/read/38526354/can-duvelisib-and-eganelisib-work-for-both-cancer-and-covid-19-molecular-level-insights-from-md-simulations-and-enhanced-samplings
#35
JOURNAL ARTICLE
Saroj Kumar Panda, Shaswata Karmakar, Parth Sarthi Sen Gupta, Malay Kumar Rana
SARS-CoV-2 has caused severe illness and anxiety worldwide, evolving into more dreadful variants capable of evading the host's immunity. Cytokine storms, led by PI3Kγ, are common in cancer and SARS-CoV-2. Naturally, there is a yearning to see whether any drugs could alleviate cytokine storms for both. Upon investigation, we identified two anticancer drugs, Duvelisib and Eganelisib, that could also work against SARS-CoV-2. This report is the first to decipher their synergic therapeutic effectiveness against COVID-19 and cancer with molecular insights from atomistic simulations...
March 25, 2024: Physical Chemistry Chemical Physics: PCCP
https://read.qxmd.com/read/38525994/substrate-selection-criteria-in-regulated-intramembrane-proteolysis
#36
REVIEW
Celine Moser, Nadja Guschtschin-Schmidt, Mara Silber, Julia Flum, Claudia Muhle-Goll
Alzheimer's disease is the most common form of dementia encountered in an aging population. Characteristic amyloid deposits of Aβ peptides in the brain are generated through cleavage of amyloid precursor protein (APP) by γ-secretase, an intramembrane protease. Cryo-EM structures of substrate γ-secretase complexes revealed details of the process, but how substrates are recognized and enter the catalytic site is still largely ignored. γ-Secretase cleaves a diverse range of substrate sequences without a common consensus sequence, but strikingly, single point mutations within the transmembrane domain (TMD) of specific substrates may greatly affect cleavage efficiencies...
March 25, 2024: ACS Chemical Neuroscience
https://read.qxmd.com/read/38525648/an-efficient-peptide-ligase-engineered-from-a-bamboo-asparaginyl-endopeptidase
#37
JOURNAL ARTICLE
Xin-Bo Wang, Cong-Hui Zhang, Teng Zhang, Hao-Zheng Li, Ya-Li Liu, Zeng-Guang Xu, Gang Lei, Chun-Ju Cai, Zhan-Yun Guo
In recent years, a few asparaginyl endopeptidases (AEPs) from certain higher plants have been identified as efficient peptide ligases with wide applications in protein labeling and cyclic peptide synthesis. Recently, we developed a NanoLuc Binary Technology (NanoBiT)-based peptide ligase activity assay to identify more AEP-type peptide ligases. Herein, we screened 61 bamboo species from 16 genera using this assay and detected AEP-type peptide ligase activity in the crude extract of all tested bamboo leaves...
March 25, 2024: FEBS Journal
https://read.qxmd.com/read/38525085/effects-of-duo-strain-probiotics-on-growth-digestion-and-gut-health-in-broiler-chickens
#38
JOURNAL ARTICLE
Seyed Mehrdad Mirsalami, Mahsa Mirsalami
The goal of this inquiry was to analyze the impact of incorporating Enterococcus faecium and Streptococcus thermophilus using a novel premix-spray method on the following aspects: growth rate, digestive enzyme activity, antioxidant levels, gut microbiome composition, and the morphological characteristics of the duodenum, jejunum, and ileum in broiler chickens. Furthermore, this study explored the potential benefits of duo strains of probiotics (DSP) in reducing flatulence, regulating stool microbial population, and improving diarrhea symptoms...
June 2024: Veterinary and Animal Science
https://read.qxmd.com/read/38524442/novel-stable-protease-inhibitor-from-phoenix-dactylifera-l-flowers-with-antimicrobial-and-antitumoral-activities
#39
JOURNAL ARTICLE
Ikram Jemel, Najeh Krayem, Abir Ben Bacha, Mona Alonazi, Habib Horchani, Hanène Ghamgui
A novel protease inhibitor isolated from date palm Phoenix dactylifera (L.) flowers (PIDF) was purified and characterized. A heat and acidic treatment step followed by ethanol precipitation and reverse-phase high-performance chromatography was applied to purify this natural protease inhibitor to homogeneity with a single band of about 19 kDa. The stability study depicted that PIDF was fully stable at 40 °C and retained 65% of its initial activity after heating at 50 °C for 24 h. Its thermal stability at 70 °C was markedly enhanced by adding calcium, bovine serum albumin, and sorbitol as well as by metal divalent cations, especially Mg2+ and Hg2+ ...
March 19, 2024: ACS Omega
https://read.qxmd.com/read/38524400/evaluation-of-the-probiotic-potential-of-yeast-isolated-from-kombucha-in-new-zealand
#40
JOURNAL ARTICLE
Boying Wang, Kay Rutherfurd-Markwick, Ninghui Liu, Xue-Xian Zhang, Anthony N Mutukumira
The current study investigated the in vitro probiotic potential of yeast isolated from kombucha, a tea beverage fermented with a symbiotic culture of acetic acid bacteria and yeast. A total of 62 yeast strains were previously isolated from four different commercial kombucha samples sold in New Zealand. Fifteen representative isolates belonging to eight different species were evaluated for their growth under different conditions (temperature, low pH, concentrations of bile salts, and NaCl). Cell surface characteristics, functional and enzymatic activities of the selected strains were also studied in triplicate experiments...
2024: Current research in food science
keyword
keyword
63649
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.