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https://www.readbyqxmd.com/read/28213914/novel-method-to-assess-intrinsic-heart-rate-recovery-in-ambulatory-ecg-recordings-tracks-cardioprotective-effects-of-chronic-autonomic-regulation-therapy-in-patients-enrolled-in-the-anthem-hf-study
#1
Gerrard M Carlson, Imad Libbus, Badri Amurthur, Bruce H KenKnight, Richard L Verrier
BACKGROUND: Postexercise heart rate recovery (HRR) is a powerful and independent predictor of mortality. Autonomic regulation therapy (ART) with chronic vagus nerve stimulation (VNS) has been shown to improve ventricular function in patients with chronic heart failure. However, the effect of ART on HRR in patients with heart failure remains unknown. METHODS: A new measure involving quantification of intrinsic HRR was developed for 24-hr ambulatory ECG (AECG) recordings based on spontaneous heart rate changes observed during daily activity in patients with symptomatic heart failure and reduced ejection fraction...
February 17, 2017: Annals of Noninvasive Electrocardiology
https://www.readbyqxmd.com/read/28213845/topical-formulation-containing-beeswax-based-nanoparticles-improved-in-vivo-skin-barrier-function
#2
Carla Souza, Luis Alexandre Pedro de Freitas, Patrícia Maria Berardo Gonçalves Maia Campos
Lipid nanoparticles have shown many advantages for treatment/prevention of skin disorders with damaged skin barrier function. Beeswax is a favorable candidate for the development of nanosystems in the cosmetic and dermatological fields because of its advantages for the development of products for topical application. In the present study, beeswax-based nanoparticles (BNs) were prepared using the hot melt microemulsion technique and incorporated to a gel-cream formulation. The formulation was subsequently evaluated for its rheological stability and effect on stratum corneum water content (SCWC) and transepidermal water loss (TEWL) using in vivo biophysical techniques...
February 17, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28213750/effect-of-an-acute-dose-of-omega-3-fish-oil-following-exercise-induced-muscle-damage
#3
J R Jakeman, D M Lambrick, B Wooley, J A Babraj, J A Faulkner
PURPOSE: The purpose of this double-blind, placebo-controlled study was to examine the effect of two fish oil supplements, one high in EPA (750 mg EPA, 50 mg DHA) and one low in EPA (150 mg EPA, 100 mg DHA), taken acutely as a recovery strategy following EIMD. METHODS: Twenty-seven physically active males (26 ± 4 year, 1.77 ± 0.07 m, 80 ± 10 kg) completed 100 plyometric drop jumps to induce muscle damage. Perceptual (perceived soreness) and functional (isokinetic muscle strength at 60° and 180° s(-1), squat jump performance and countermovement jump performance) indices of EIMD were recorded before, and 1, 24, 48, 72, and 96h after the damaging protocol...
February 17, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28213666/patterns-of-abnormal-gastric-pacemaking-after-sleeve-gastrectomy-defined-by-laparoscopic-high-resolution-electrical-mapping
#4
Rachel Berry, Leo K Cheng, Peng Du, Niranchan Paskaranandavadivel, Timothy R Angeli, Terence Mayne, Grant Beban, Gregory O'Grady
BACKGROUND: Laparoscopic sleeve gastrectomy (LSG) is increasingly being applied to treat obesity. LSG includes excision of the normal gastric pacemaker, which could induce electrical dysrhythmias impacting on post-operative symptoms and recovery, but these implications have not been adequately investigated. This study aimed to define the effects of LSG on gastric slow-wave pacemaking using laparoscopic high-resolution (HR) electrical mapping. METHODS: Laparoscopic HR mapping was performed before and after LSG using flexible printed circuit arrays (64-96 electrodes; 8-12 cm(2); n = 8 patients) deployed through a 12 mm trocar and positioned on the gastric serosa...
February 17, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28213326/amino-acid-mediated-turn-off-turn-on-nanozyme-activity-of-gold-nanoclusters-for-sensitive-and-selective-detection-of-copper-ions-and-histidine
#5
Yan Liu, Ding Ding, Yuanlin Zhen, Rong Guo
Herein, we presented a facile strategy for highly sensitive and selective detection of both Cu(2+) and histidine (His) by combining the peroxidase-like nanozyme activity of gold nanoclusters with amino acid's ambidentate nature. The peroxidase-like catalytic ability of histidine-Au nanoclusters (His-AuNCs) can be inhibited by the addition of Cu(2+). The sensitivity of this probe to Cu(2+) is significant with a linear range of 1-100nM, and a low detection limit of 0.1nM. More interestingly, His-AuNC/Cu(2+) undergoes recovery of the activity upon exposure to free His, because His/Cu(2+) complex is more stable due to the participation of the imidazole ring of His...
January 19, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28213251/on-line-monitoring-of-in-vitro-oral-bioaccessibility-tests-as-front-end-to-liquid-chromatography-for-determination-of-chlorogenic-acid-isomers-in-dietary-supplements
#6
Daniel Kremr, David J Cocovi-Solberg, Petra Bajerová, Karel Ventura, Manuel Miró
A novel fully automated in-vitro oral dissolution test assay as a front-end to liquid chromatography has been developed and validated for on-line chemical profiling and monitoring of temporal release profiles of three caffeoylquinic acid (CQA) isomers, namely, 3-CQA,4-CQA and 5-CQA, known as chlorogenic acids, in dietary supplements. Tangential-flow filtration is harnessed as a sample processing approach for on-line handling of CQA containing extracts of hard gelatin capsules and introduction of protein-free samples into the liquid chromatograph...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213226/a-feasible-method-for-indirect-quantification-of-l-t4-in-drugs-by-iodine-determination
#7
Marcia Foster Mesko, Alessandra Cortes Teotonio, Dirce Taina Teixeira Oliveira, Diogo La Rosa Novo, Vanize Caldeira Costa
In this work, a method combining microwave-induced combustion (MIC) for sample preparation of commercial levothyroxine sodium (L-T4) drugs (L-T4: 25-200µg/tablet), and potentiometry with ion selective electrode (ISE) for iodine determination and subsequent indirect quantification of L-T4 was proposed. The type and concentration of the absorbing solution were evaluated to select the most suitable conditions for this study. Using the MIC method, it was possible to use solutions as diluted as 150mmolL(-1) (NH4)2CO3 (for samples containing 25-200µg of L-T4/tablet) for I absorption...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213127/quantitation-of-lead-210-210-pb-using-lead-203-203-pb-as-a-massless-yield-tracer
#8
D May, A N Nelson, M K Schultz
Determination of Pb-210 ((210)Pb) in aqueous solution is a common radioanalytical challenge in environmental science. Widely used methods for undertaking these analyses (e.g., ASTM D7535) rely on the use of stable lead (Pb) as a yield tracer that takes into account losses of (210)Pb that inevitably occur during elemental/radiochemical separations of the procedures. Although effective, these methods introduce technical challenges that can be difficult to track and potentially introduce uncertainty that can be difficult to quantify...
February 14, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28212510/impact-of-paint-shop-decanter-effluents-on-biological-treatability-of-automotive-industry-wastewater
#9
Didem Güven, Oytun Hanhan, Elif Ceren Aksoy, Güçlü Insel, Emine Çokgör
A lab-scale Sequencing Batch Reactor (SBR) was implemented to investigate biological treatability and kinetic characteristics of paint shop wastewater (PSW) together with main stream wastewater (MSW) of a bus production factory. Readily biodegradable and slowly biodegradable COD fractions of MWS were determined by respirometric analysis: 4.2% (SS), 10.4% (SH) and 59.3% (XS). Carbon and nitrogen removal performance of the SBR feeding with MSW alone were obtained as 89% and 58%, respectively. When PSW was introduced to MSW, both carbon and nitrogen removal were deteriorated...
January 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28212395/delayed-apoptosis-allows-islet-%C3%AE-cells-to-implement-an-autophagic-mechanism-to-promote-cell-survival
#10
Heather L Hayes, Brett S Peterson, Jonathan M Haldeman, Christopher B Newgard, Hans E Hohmeier, Samuel B Stephens
Increased β-cell death coupled with the inability to replicate existing β-cells drives the decline in β-cell mass observed in the progression of both major forms of diabetes. Understanding endogenous mechanisms of islet cell survival could have considerable value for the development of novel strategies to limit β-cell loss and thereby promote β-cell recovery. Insulinoma cells have provided useful insight into β-cell death pathways but observations made in cell lines sometimes fail to translate to primary islets...
2017: PloS One
https://www.readbyqxmd.com/read/28211497/the-inflammatory-cytokine-tnf-%C3%AE-promotes-the-premature-senescence-of-rat-nucleus-pulposus-cells-via-the-pi3k-akt-signaling-pathway
#11
Pei Li, Yibo Gan, Yuan Xu, Lei Song, Liyuan Wang, Bin Ouyang, Chengmin Zhang, Qiang Zhou
Premature senescence of nucleus pulposus (NP) cells and inflammation are two common features of degenerated discs. This study investigated the effects of the inflammatory cytokine TNF-α on the premature senescence of NP cells and the molecular mechanism behind this process. Rat NP cells were cultured with or without different concentrations of TNF-α for 1 and 3 days. The inhibitor LY294002 was used to determine the role of the PI3K/Akt pathway. NP cells that were incubated with TNF-α for 3 days followed by 3 days of recovery in the control medium were used to analyze cellular senescence...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28211495/characterization-of-biocarbon-source-recovery-and-microbial-community-shifts-from-waste-activated-sludge-by-conditioning-with-cornstover-assessment-of-cellulosic-compositions
#12
Kaili Wen, Aijuan Zhou, Jiaguang Zhang, Zhihong Liu, Guoying Wang, Wenzong Liu, Aijie Wang, Xiuping Yue
Most studies on the production of volatile fatty acids (VFAs) from waste activated sludge (WAS) digestion have focused on operating conditions, pretreatments and characteristic adjustments. Conditioning by extra carbon sources (ECS), normally added in a solid form, has been reported to be an efficient approach. However, this has caused considerable waste of monomeric sugars in the hydrolysate. In this study, the effects of two added forms (pretreated straw (S) and hydrolyzed liquid (L)) of cornstover (CS) on WAS acidification were investigated...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210899/interleukin-6-as-possible-early-marker-of-stress-response-after-femoral-fracture
#13
Goran Pesic, Jovana Jeremic, Tamara Nikolic, Vladimir Zivkovic, Ivan Srejovic, Aleksandra Vranic, Jovana Bradic, Branko Ristic, Aleksandar Matic, Nikola Prodanovic, Vladimir Jakovljevic
Bone fracture healing is a complex process which at best results in full recovery of function and structure of injured bone tissue, but all the mechanisms involved in this process, and their mutual interaction, are not fully understood. Despite advancement of surgical procedures, this type of fractures is still a major public health concern. In the last few decades, a lot of attention is focused on the oxygen-free radicals and inflammatory response markers as important factors of skeletal injury. Thus, the aim of the present study was to follow the changes in redox balance and inflammatory response in elderly patients with femoral fractures during the earliest stages of fracture healing, by measuring the values of the observed markers immediately after fracture, as well as the first, third, and seventh postoperative day...
February 16, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28210842/uric-acid-as-a-novel-biomarker-for-bone-marrow-function-and-incipient-hematopoietic-reconstitution-after-aplasia-in-patients-with-hematologic-malignancies
#14
Sebastian P Haen, Vicky Eyb, Nora Mirza, Aline Naumann, Andreas Peter, Markus W Löffler, Christoph Faul, Wichard Vogel, Wolfgang A Bethge, Hans-Georg Rammensee, Lothar Kanz, Martin Heni
PURPOSE: Prolonged aplasia and graft failure (GF) represent life-threatening complications after hematopoietic cell transplantation (HCT) requiring suitable biomarkers for early detection and differentiation between GF and poor graft function (PGF). Uric acid (UA) is a strong immunological danger signal. METHODS: Laboratory results were analyzed from patients undergoing either allogeneic or autologous HCT or induction chemotherapy for acute leukemia (n = 50 per group, n = 150 total)...
February 16, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28210257/kinetics-of-cytotoxic-lymphocytes-reconstitution-after-induction-chemotherapy-in-elderly-aml-patients-reveals-progressive-recovery-of-normal-phenotypic-and-functional-features-in-nk-cells
#15
Jérôme Rey, Cyril Fauriat, Eloïse Kochbati, Florence Orlanducci, Aude Charbonnier, Evelyne D'Incan, Pascale Andre, François Romagne, Bernadette Barbarat, Norbert Vey, Daniel Olive
NK cells are defective in acute myeloid leukemia (AML) at diagnosis. Here, we studied the kinetic of expression of the major activating and inhibitory receptors of NK, CD8 T, and γδ T cells in patients undergoing chemotherapy (CT) for the treatment of AML (n = 29). We showed that NK cells are the main affected population at diagnosis and that expression of activating receptors is partially restored within a few weeks after CT. CD8 T cells and γδ T cells are only weakly affected at diagnosis. Killer cell immunoglobulin-like receptor expression by NK cells, but not NKG2A and CD85j, was downregulated...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28210068/use-of-an-arm-weight-bearing-combined-with-upper-limb-reaching-apparatus-to-facilitate-motor-paralysis-recovery-in-an-incomplete-spinal-cord-injury-patient-a-single-case-report
#16
Takashi Hoei, Kazumi Kawahira, Hidefumi Fukuda, Keizo Sihgenobu, Megumi Shimodozono, Tadashi Ogura
[Purpose] Training using an arm weight-bearing device combined with upper-limb reaching apparatus to facilitate motor paralysis recovery, named the "Reaching Robot", as well as Repetitive Facilitation Exercise were applied to a patient with severe impairment of the shoulder and elbow due to incomplete spinal cord injury and the effects were examined. [Subjects and Methods] A 66-year-old man with incomplete spinal cord injury participated in an upper extremity rehabilitation program involving a Reaching Robot...
January 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28210051/effects-of-two-handed-task-training-on-upper-limb-function-of-chronic-hemiplegic-patients-after-stroke
#17
Byung Il Yang, Bo Kyoung Song, Sang Mi Joung
[Purpose] The purpose of this study was to determine whether two-handed task training is effective on motor learning of injured cerebral cortex activation and upper extremity function recovery after stroke. [Subjects and Methods] Two hemiplegic subjects participated in this study: one patient was affected on the dominant side of the body and the other was affected on the non-dominant side of the body, and both scored in the range of 58-66 in the Fugl-Meyer assessment. The excitability of the corticospinal tract and Manual Function Test were examined...
January 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28209627/characterization-of-a-blood-spot-creatine-kinase-skeletal-muscle-isoform-immunoassay-for-high-throughput-newborn-screening-of-duchenne-muscular-dystrophy
#18
Stuart J Moat, Teemu Korpimäki, Petra Furu, Harri Hakala, Hanna Polari, Liisa Meriö, Pauliina Mäkinen, Ian Weeks
BACKGROUND: Duchenne muscular dystrophy (DMD) is a progressive, lethal X-linked neuromuscular disorder with an average worldwide incidence of 1:5000. Blood spot creatine kinase (CK) enzyme assays previously used in newborn screening programs for DMD are nonspecific because measured CK enzyme activity is attributable to 3 isoenzyme forms of CK (CK-MM, CK-MB, and CK-BB) and it is the CK-MM isoform that is found predominantly in skeletal muscle. CK-MM is increased in boys with DMD owing to muscle damage...
February 16, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28209619/lapatinib-resistance-in-breast-cancer-cells-is-accompanied-by-phosphorylation-mediated-reprogramming-of-glycolysis
#19
Benjamin Ruprecht, Esther A Zaal, Jana Zecha, Wei Wu, Celia R Berkers, Bernhard Kuster, Simone Lemeer
HER2/ERBB2-overexpressing breast cancers targeted effectively by the small molecule kinase inhibitor lapatinib frequently acquire resistance to this drug. In this study, we employed explorative mass spectrometry to profile proteomic, kinomic, and phosphoproteomic changes in an established model of lapatinib resistance to systematically investigate initial inhibitor response and subsequent reprogramming in resistance. The resulting dataset, which collectively contains quantitative data for >7,800 proteins, >300 protein kinases and >15,000 phosphopeptides, enabled deep insight into signaling recovery and molecular reprogramming upon resistance...
February 16, 2017: Cancer Research
https://www.readbyqxmd.com/read/28209521/impaired-visual-competition-in-patients-with-homonymous-visual-field-defects
#20
A C Geuzebroek, A V van den Berg
Intense visual training can lead to partial recovery of visual field defects caused by lesions of the primary visual cortex. However, the standard visual detection and discrimination tasks, used to assess this recovery process tend to ignore the complexity of the natural visual environment, where multiple stimuli continuously interact. Visual competition is an essential component for natural search tasks and detecting unexpected events. Our study focused on visual decision-making and to what extent the recovered visual field can compete for attention with the 'intact' visual field...
February 13, 2017: Neuropsychologia
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