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https://www.readbyqxmd.com/read/28735419/modulation-of-human-kv4-3-kchip2-channel-inactivation-kinetics-by-cytoplasmic-ca-2
#1
Christiane Groen, Robert Bähring
The transient outward current (I to) in the human heart is mediated by Kv4.3 channels complexed with Kv channel interacting protein (KChIP) 2, a cytoplasmic Ca(2+)-binding EF-hand protein known to modulate Kv4.3 inactivation gating upon heterologous co-expression. We studied Kv4.3 channels co-expressed with wild-type (wt) or EF-hand-mutated (ΔEF) KChIP2 in human embryonic kidney (HEK) 293 cells. Co-expression took place in the absence or presence of BAPTA-AM, and macroscopic currents were recorded in the whole-cell patch-clamp configuration with different free Ca(2+) concentrations in the patch-pipette...
July 22, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28735224/simultaneous-quantification-of-cortisol-and-cortisone-in-urines-from-infants-with-packed-fiber-solid-phase-extraction-coupled-to-hplc-ms-ms
#2
Chen Li, Zhao Zhang, Xiongwei Liu, Kangwei Shen, Pan Gu, Xuejun Kang
Cortisol and cortisone are two important glucocorticoids in human body, their interconversion is controlled by two isotypes of 11β-hydroxy steroid dehydrogenase (11β-HSD1 and 11β-HSD2). The ratio of urinary cortisol to cortisone can be used to assess the activity of 11β-HSDs. An analytical method to quantify urinary cortisol and cortisone using high performance liquid chromatographic tandem mass spectrometry following a packed-fiber solid-phase extraction (PFSPE) was developed. The proposed method was validated and applied to determine the urinary cortisol and cortisone concentrations in infants...
July 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28735060/individual-roles-of-brain-and-serum-alcohol-dehydrogenase-isoforms-in-regulation-of-alcohol-consumption-in-spf-wistar-rats
#3
Vsevolod V Pavshintsev, Nikita A Mitkin, Olga Y Frolova, Ekaterina A Kushnir, Olga A Averina, Maxim L Lovat
Alcohol dehydrogenases (ADH) are key enzymes of ethanol metabolism that mediate its oxidation to acetaldehyde. ADHs are also able to oxidize some types of neurotransmitters such as dopamine, serotonin and norepinephrine. Increased level of ADHs activity, induced by chronic alcohol consumption, is presumably associated with disturbed neurotransmitters metabolism that leads to stable alcohol craving. As earlier reported, intraperitoneal administration of 4-methilpirasole (non-specific inhibitor of ADHs) has shown to provide a short-term anti-alcoholic effect, but individual roles of ADH isoforms in this process were still unclear...
July 19, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28735026/regulated-viral-bdnf-delivery-in-combination-with-schwann-cells-promotes-axonal-regeneration-through-capillary-alginate-hydrogels-after-spinal-cord-injury
#4
Shengwen Liu, Beatrice Sandner, Thomas Schackel, LaShae Nicholson, Abdelwahed Chtarto, Liliane Tenenbaum, Radhika Puttagunta, Rainer Müller, Norbert Weidner, Armin Blesch
Grafting of cell-seeded alginate capillary hydrogels into a spinal cord lesion site provides an axonal bridge while physically directing regenerating axonal growth in a linear pattern. However, without an additional growth stimulus, bridging axons fail to extend into the distal host spinal cord. Here we examined whether a combinatory strategy would support regeneration of descending axons across a cervical (C5) lateral hemisection lesion in the rat spinal cord. Following spinal cord transections, Schwann cell (SC)-seeded alginate hydrogels were grafted to the lesion site and AAV5 expressing brain-derived neurotrophic factor (BDNF) under control of a tetracycline-regulated promoter was injected caudally...
July 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28734986/exogenous-hedgehog-antagonist-delays-but-does-not-prevent-fracture-healing-in-young-mice
#5
Xiaochen Liu, Jennifer A McKenzie, Clayton W Maschhoff, Michael J Gardner, Matthew J Silva
Fracture healing recapitulates many aspects of developmental osteogenesis. The hedgehog (Hh) signaling pathway, essential to skeletal development, is upregulated during fracture healing, although its importance is unclear. Our goal was to assess the functional importance of Hh signaling in endochondral fracture healing. We created closed, transverse diaphyseal femur fractures in mice, stabilized with an intramedullary pin, and administered a systemic Hh inhibitor or vehicle. Because Hh pathway activation is mediated by the receptor Smoothened (Smo), we used the Smo antagonist GDC-0449 (GDC, 50mg/kg, twice daily) to target the pathway...
July 19, 2017: Bone
https://www.readbyqxmd.com/read/28734773/epico-4-0-total-quality-in-the-management-of-invasive-candidiasis-in-critically-ill-patients-by-analysing-the-integrated-process
#6
Rafael Zaragoza, Ricard Ferrer, Pedro Llinares, Emilio Maseda, Alejandro Rodríguez, Santiago Grau, Guillermo Quindós
BACKGROUND: A high quality integrated process in the clinical setting of non-neutropenic critically ill patients at risk for invasive candidiasis is a necessary tool to improve the management of these patients. AIMS: To identify the key points on invasive candidiasis in order to develop a set of recommendations with a high level of consensus required for the creation of a total quality integrated process for the management of non-neutropenic critically ill patients at risk of invasive candidiasis...
July 19, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28734356/palladium-copper-nanowires-based-biosensor-for-the-ultrasensitive-detection-of-organophosphate-pesticides
#7
Dandan Song, Yan Li, Xiong Lu, Muxue Sun, Hui Liu, Guangming Yu, Faming Gao
A highly sensitive acetylcholinesterase (AChE) electrochemical biosensor for the quantitative determination of organophosphate pesticides (OPs) in vegetables and fruits based on palladium-copper nanowires (Pd-Cu NWs) was reported. AChE immobilized on the modified electrode could catalyze hydrolysis of acetylthiocholine chloride (ATCl), generating an irreversible oxidation peak. When exposed to the OPs, the activity of the AChE was inhibited and the current significantly decreased. The detection mechanism is based on the inhibition of AChE...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28733237/a-critical-test-of-drosophila-anaesthetics-isoflurane-and-sevoflurane-are-benign-alternatives-to-cold-and-co2
#8
Heath A MacMillan, Mikkel Nørgård, Heidi J MacLean, Johannes Overgaard, Catherine J A Williams
Anaesthesia is often a necessary step when studying insects like the model organism Drosophila melanogaster. Most studies of Drosophila and other insects that require anaesthesia use either cold exposure or carbon dioxide exposure to induce a narcotic state. These anaesthetic methods are known to disrupt physiology and behavior with increasing exposure, and thus ample recovery time is required prior to experimentation. Here, we examine whether two halogenated ethers commonly used in vertebrate anaesthesia, isoflurane and sevoflurane, may serve as alternative means of insect anaesthesia...
July 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28732957/sonochemical-synthesis-of-silver-nanoparticles-anchored-reduced-graphene-oxide-nanosheets-for-selective-and-sensitive-detection-of-glutathione
#9
Victor Vinoth, Jerry J Wu, Abdullah M Asiri, Sambandam Anandan
Developed here an eco-friendly, one-pot approach toward rapid synthesis of silver nanoparticles anchored reduced graphene oxide (AgNPs(TMSPED)-rGO) nanosheets via sonochemical irradiation method, using an aqueous solution mixture of GO and AgNO3 in the presence of N-[3(trimethoxysilyl)propyl] ethylenediamine (TMSPED) without any reducing agent. As synthesized decorated nanosheets was thoroughly characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) spectroscopy, Raman spectroscopy and X-ray photoelectron spectroscopy (XPS)...
November 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28732218/invasiveness-of-tibial-bone-graft-harvesting-for-secondary-alveolar-bone-grafting-can-harvesting-be-made-at-the-age-of-less-than-10%C3%A2-years-without-complications
#10
Kohta Fukuta, Kenichi Kurita, Atsushi Nakayama, Masahito Maeda, Hiroshi Watanabe, Mitsuo Goto
PURPOSE: In this study, particulate cancellous bone marrow was harvested from the tibia for alveolar bone grafting, and postoperative complications at the donor site were compared between patients aged 10 years or older (29 tibias) and those aged younger than 10 years (42 tibias). PATIENTS AND METHODS: Enrolled patients were those who underwent tibial bone graft harvesting at the Department of Oral and Maxillofacial Surgery, Aichi Gakuin University, during a period of 3 years and 1 month from March 2012 through March 2015...
June 24, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28732171/using-metagenomics-to-show-the-efficacy-of-forest-restoration-in-the-new-jersey-pine-barrens
#11
William D Eaton, Shadi Shokralla, Kathleen M McGee, Mehrdad Hajibabaei
The Franklin Parker Preserve within the New Jersey Pine Barrens contains 5,000 acres of wetlands habitat, including old-growth Atlantic White Cedar (or AWC; Chamaecyparis thyoides) swamps, cranberry bogs, and former cranberry bogs undergoing restoration into AWC forests. This study showed that the C-use efficiency was greater in the old-growth AWC soils than in soils from 8-year old mid-stage restored AWC stands, which were greater than found in soil from 4-year old AWC stands-the latter two stands being restored from long-term cranberry bogs...
July 21, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28732151/patients-attitudes-and-experiences-of-disease-modifying-anti-rheumatic-drugs-in-rheumatoid-arthritis-and-spondyloarthritis-a-qualitative-synthesis
#12
Ayano Kelly, Kathleen Tymms, David J Tunnicliffe, Daniel Sumpton, Chandima Perera, Kieran Fallon, Jonathan C Craig, Walter Abhayaratna, Allison Tong
OBJECTIVES: Non-adherence to disease-modifying anti-rheumatic drugs (DMARDS) in rheumatoid arthritis (RA) and spondyloarthritis (SpA) results in increased disease activity and symptoms and poorer quality of life. We aimed to describe patients' attitudes and experiences of DMARDs in RA and SpA to inform strategies to improve medication adherence. METHODS: Databases (MEDLINE, Embase, PsycINFO, CINAHL) were searched to January 2016. Thematic synthesis was used to analyze the findings...
July 21, 2017: Arthritis Care & Research
https://www.readbyqxmd.com/read/28732117/identification-by-phenotypic-and-genetic-approaches-of-an-indigenous-saccharomyces-cerevisiae-wine-strain-with-high-desiccation-tolerance
#13
Marianna Zambuto, Rossana Romaniello, Nicoletta Guaragnella, Patrizia Romano, Sonia Votta, Angela Capece
During active dry yeast (ADY) production process, cells are exposed to multiple stresses, such as thermal, oxidative and hyperosmotic shock. Previously, by analyzing cells in exponential growth phase, we selected an indigenous Saccharomyces cerevisiae wine strain, namely CD-6Sc, for its higher tolerance to desiccation and higher expression of specific desiccation stress-related genes in comparison to other yeast strains. In this study, we performed a desiccation treatment on stationary phase cells by comparing the efficacy of two different methods: a "laboratory dry test" in a small-scale (mild stress) and a treatment by spray-drying (severe stress), one of the most appropriate preservation method for yeasts and other micro-organisms...
July 21, 2017: Yeast
https://www.readbyqxmd.com/read/28731864/minimal-detectable-change-thresholds-and-responsiveness-of-zephyr-bioharness-fitbit-charge-devices
#14
Goris Nazari, Joy C MacDermid
The aim of the current study was to define the MDC thresholds for Zephyr Bioharness (ZB) and Fitbit Charge (FC-HR) heart rate variables, and to establish the internal responsiveness of ZB and FC-HR devices in terms of their heart rate, respiratory rate variables and number of steps taken variables, in a healthy sample of males and females at resting, sub-maximal activity and throughout recovery phases. A total of sixty participants (30 females, 48 ± 15) and (30 males, 48 ± 15) were recruited using stratified convenience and snowball sampling approaches from our university student, staff and faculty population...
July 18, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28731517/mental-steps-differential-activation-of-internal-pacemakers-in-motor-imagery-and-in-mental-imitation-of-gait
#15
Lucia Maria Sacheli, Laura Zapparoli, Carlo De Santis, Matteo Preti, Catia Pelosi, Nicola Ursino, Alberto Zerbi, Giuseppe Banfi, Eraldo Paulesu
Gait imagery and gait observation can boost the recovery of locomotion dysfunctions; yet, a neurologically justified rationale for their clinical application is lacking as much as a direct comparison of their neural correlates. Using functional magnetic resonance imaging, we measured the neural correlates of explicit motor imagery of gait during observation of in-motion videos shot in a park with a steady cam (Virtual Walking task). In a 2 × 2 factorial design, we assessed the modulatory effect of gait observation and of foot movement execution on the neural correlates of the Virtual Walking task: in half of the trials, the participants were asked to mentally imitate a human model shown while walking along the same route (mental imitation condition); moreover, for half of all the trials, the participants also performed rhythmic ankle dorsiflexion as a proxy for stepping movements...
July 21, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28731316/phytochemical-analysis-of-myrtus-communis-plant-conventional-versus-microwave-assisted-extraction-procedures
#16
Nadia Bouaoudia-Madi, Lila Boulekbache-Makhlouf, Nabil Kadri, Farid Dahmoune, Hocine Remini, Sofiane Dairi, Sonia Oukhmanou-Bensidhoum, Khodir Madani
Background Myrtle (Myrtus communis L) may constitute an interesting dietary source of health protective compounds. Microwave-assisted extraction (MAE) of total phenolic compounds (TPC) from myrtle leaf, stems, pericarp, and seeds was studied and the results were compared with those of the conventional method extraction (CME) in terms of extraction time. Methods Extraction yield/efficiency and antioxidant activity were measured using radical scavenging assay (DPPH•) and reducing power. Results The results show that the MAE was higher in terms of saving energy, extraction time (62 s) and extraction efficiency of bioactive compound compared to CME (2 h)...
June 10, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28731131/assessment-of-the-antioxidant-activity-of-an-olive-oil-total-polyphenolic-fraction-and-hydroxytyrosol-from-a-greek-olea%C3%A2-europea-variety-in-endothelial-cells-and-myoblasts
#17
Paraskevi Kouka, Alexandros Priftis, Dimitrios Stagos, Apostolis Angelis, Panagiotis Stathopoulos, Nikos Xinos, Alexios-Léandros Skaltsounis, Charalampos Mamoulakis, Aristides M Tsatsakis, Demetrios A Spandidos, Demetrios Kouretas
Olive oil (OO) constitutes the basis of the Mediterranean diet, and it seems that its biophenols, such as hydroxytyrosol (HT) may scavenge free radicals, attracting distinct attention due to their beneficial effects in many pathological conditions, such as cancer. To the best of our knowedge, this is the first study in which the functional properties of an OO total polyphenolic fraction (TPF) and pure HT were examined in order to determine their antioxidant effects at a cellular level in endothelial cells and myoblasts...
July 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28730870/cardiac-autonomic-recovery-after-strength-exercise-in-lower-and-upper-limbs
#18
Naerton José Xavier Isidoro, Milana Drumond Ramos Santana, Vitor E Valenti, David M Garner, Luiz Carlos de Abreu
Introduction Strength exercises influence the cardiovascular system by promoting autonomic adjustments induced by the increased metabolic demand and consequent increase in local blood flow. However, it is unclear whether there are differences between upper and lower limbs. We aimedto evaluate recovery of cardiac autonomic modulation afterstrengthexercise in upper and lower limbs. Methods We analysed 29 healthy male subjects physically active but not professional athletes aged between 20 and 35 years old...
July 21, 2017: Acta Cardiologica
https://www.readbyqxmd.com/read/28729917/autophagy-endoplasmic-reticulum-stress-and-the-unfolded-protein-response-in-intracerebral-hemorrhage
#19
Mingming Niu, Xiaohong Dai, Wei Zou, Xueping Yu, Wei Teng, Qiuxin Chen, Xiaowei Sun, Weiwei Yu, Huihui Ma, Peng Liu
Intracerebral hemorrhage (ICH) is a subtype of stroke that is followed by primary and secondary brain injury. As a result of the injury, cell metabolism is disrupted and a series of stress responses are activated, such as endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), leading to the re-establishment of cell homeostasis or cell death. As an important mechanism of cell homeostasis, autophagy has been widely studied, and the associations between autophagy, ER stress, and the UPR have also been demonstrated...
2017: Translational Neuroscience
https://www.readbyqxmd.com/read/28729835/uplc-esi-ms-ms-and-hptlc-method-for-quantitative-estimation-of-cytotoxic-glycosides-and-aglycone-in-bioactivity-guided-fractions-of-solanum-nigrum-l
#20
Karishma Chester, Sarvesh Paliwal, Washim Khan, Sayeed Ahmad
Solanum nigrum L., is traditionally used for the management of the various liver disorders. Investigating the effect of polarity based fractionation of S. nigrum for its hepatoprotective effect on Hep G2 cells in vitro to provide base of its activity by quantifying in steroidal glycosides responsible for hepatoprotective potential. A new UPLC-ESI-MS/MS method following a high performance thin layer chromatography (HPTLC) has been developed and validated for quantification of steroidal glycosides and aglycone (solasonine, solamargine, and solasodine, respectively)...
2017: Frontiers in Pharmacology
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