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https://www.readbyqxmd.com/read/27807433/flhf-is-required-for-swarming-motility-and-full-pathogenicity-of-bacillus-cereus
#1
Diletta Mazzantini, Francesco Celandroni, Sara Salvetti, Sokhna A Gueye, Antonella Lupetti, Sonia Senesi, Emilia Ghelardi
Besides sporulation, Bacillus cereus can undergo a differentiation process in which short swimmer cells become elongated and hyperflagellated swarmer cells that favor migration of the bacterial community on a surface. The functionally enigmatic flagellar protein FlhF, which is the third paralog of the signal recognition particle (SRP) GTPases Ffh and FtsY, is required for swarming in many bacteria. Previous data showed that FlhF is involved in the control of the number and positioning of flagella in B. cereus...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27573015/hubp-a-polar-landmark-protein-regulates-flagellar-number-by-assisting-in-the-proper-polar-localization-of-flhg-in-vibrio-alginolyticus
#2
Norihiro Takekawa, Soojin Kwon, Noriko Nishioka, Seiji Kojima, Michio Homma
The marine bacterium Vibrio alginolyticus has a single polar flagellum, whose number is positively regulated by FlhF, and negatively by FlhG. FlhF is intrinsically localized at the cell pole, whereas FlhG localizes there through the putative interactions with the polar landmark protein HubP. Here, we focused on the role of HubP in the regulation of flagellar number in V. alginolyticus The deletion of hubP increases flagellar number, and completely disrupts the polar localization of FlhG. It had been believed that flagellar number is primarily determined by the absolute amount of FlhF localized at the cell pole...
August 29, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27379216/synthetic-cystic-fibrosis-sputum-medium-regulates-flagellar-biosynthesis-through-the-flhf-gene-in-burkholderia-cenocepacia
#3
Brijesh Kumar, Silvia T Cardona
Burkholderia cenocepacia belongs to the Burkholderia cepacia complex (Bcc), a group of at least 18 distinct species that establish chronic infections in the lung of people with the genetic disease cystic fibrosis (CF). The sputum of CF patients is rich in amino acids and was previously shown to increase flagellar gene expression in B. cenocepacia. We examined flagellin expression and flagellar morphology of B. cenocepacia grown in synthetic cystic fibrosis sputum medium (SCFM) compared to minimal medium. We found that CF nutritional conditions induce increased motility and flagellin expression...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27190143/biofilm-formation-defective-mutants-in-pseudomonas-putida
#4
Aroa López-Sánchez, Antonio Leal-Morales, Lorena Jiménez-Díaz, Ana I Platero, Juan Bardallo-Pérez, Alberto Díaz-Romero, Rafael D Acemel, Juan M Illán, Julia Jiménez-López, Fernando Govantes
Out of 8000 candidates from a genetic screening for Pseudomonas putida KT2442 mutants showing defects in biofilm formation, 40 independent mutants with diminished levels of biofilm were analyzed. Most of these mutants carried insertions in genes of the lap cluster, whose products are responsible for synthesis, export and degradation of the adhesin LapA. All mutants in this class were strongly defective in biofilm formation. Mutants in the flagellar regulatory genes fleQ and flhF showed similar defects to that of the lap mutants...
July 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/26235439/the-role-of-flhf-and-hubp-as-polar-landmark-proteins-in-shewanella-putrefaciens%C3%A2-cn-32
#5
Florian Rossmann, Susanne Brenzinger, Carina Knauer, Anja K Dörrich, Sebastian Bubendorfer, Ulrike Ruppert, Gert Bange, Kai M Thormann
Spatiotemporal regulation of cell polarity plays a role in many fundamental processes in bacteria and often relies on 'landmark' proteins which recruit the corresponding clients to their designated position. Here, we explored the localization of two multi-protein complexes, the polar flagellar motor and the chemotaxis array, in Shewanella putrefaciens CN-32. We demonstrate that polar positioning of the flagellar system, but not of the chemotaxis system, depends on the GTPase FlhF. In contrast, the chemotaxis array is recruited by a transmembrane protein which we identified as the functional ortholog of Vibrio cholerae HubP...
November 2015: Molecular Microbiology
https://www.readbyqxmd.com/read/26195616/how-bacteria-maintain-location-and-number-of-flagella
#6
REVIEW
Jan S Schuhmacher, Kai M Thormann, Gert Bange
Bacteria differ in number and location of their flagella that appear in regular patterns at the cell surface (flagellation pattern). Despite the plethora of bacterial species, only a handful of these patterns exist. The correct flagellation pattern is a prerequisite for motility, but also relates to biofilm formation and the pathogenicity of disease-causing flagellated bacteria. However, the mechanisms that maintain location and number of flagella are far from being understood. Here, we review our knowledge on mechanisms that enable bacteria to maintain their appropriate flagellation pattern...
November 2015: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/26194016/investigation-into-flhfg-reveals-distinct-features-of-flhf-in-regulating-flagellum-polarity-in-shewanella-oneidensis
#7
Tong Gao, Miaomiao Shi, Lili Ju, Haichun Gao
Rod-shaped bacterial cells are polarized, with many organelles confined to a polar cellular site. In polar flagellates, FlhF and FlhG, a multiple-domain (B-N-G) GTPase and a MinD-like ATPase respectively, function as a cognate pair to regulate flagellar localization and number as revealed in Vibrio and Pseudomonas species. In this study, we show that FlhFG of Shewanella oneidensis (SoFlhFG), a monotrichous γ-proteobacterium renowned for respiratory diversity, also play an important role in the flagellar polar placement and number control...
October 2015: Molecular Microbiology
https://www.readbyqxmd.com/read/26112286/the-mind-homolog-flhg-regulates-the-synthesis-of-the-single-polar-flagellum-of-vibrio-alginolyticus
#8
Hiroki Ono, Akari Takashima, Hikaru Hirata, Michio Homma, Seiji Kojima
FlhG, a MinD homolog and an ATPase, is known to mediate the formation of the single polar flagellum of Vibrio alginolyticus together with FlhF. FlhG and FlhF work antagonistically, with FlhF promoting flagellar assembly and FlhG inhibiting it. Here, we demonstrate that purified FlhG exhibits a low basal ATPase activity. As with MinD, the basal ATPase activity of FlhG can be activated and the D171A residue substitution enhances its ATPase activity sevenfold. FlhG-D171A localizes strongly at the cell pole and severely inhibits motility and flagellation, whereas the FlhG K31A and K36Q mutants, which are defective in ATP binding, do not localize to the poles, cannot complement a flhG mutant and lead to hyperflagellation...
October 2015: Molecular Microbiology
https://www.readbyqxmd.com/read/26018545/formation-of-a-single-polar-flagellum-by-two-distinct-flagellar-gene-sets-in-sphingomonas-sp-strain-a1
#9
Yukie Maruyama, Masahiro Kobayashi, Kousaku Murata, Wataru Hashimoto
Gram-negative Sphingomonas sp. strain A1, originally identified as a non-motile and aflagellate bacterium, possesses two sets of genes required for flagellar formation. In this study, we characterized the flagellar genes and flagellum formation in strain A1. Flagellar gene cluster set I contained 35 flagellar genes, including one flagellin gene (p6), where the gene assembly structure resembled that required for the formation of lateral flagella in gammaproteobacteria. The set II flagellar genes were arranged in eight shorter clusters with 46 flagellar genes, including two flagellin genes (p5 and p5') and flhF, which is required for polar flagella...
August 2015: Microbiology
https://www.readbyqxmd.com/read/25413671/reactions-of-chicken-sera-to-recombinant-campylobacter-jejuni-flagellar-proteins
#10
Hung-Yueh Yeh, Kelli L Hiett, John E Line
Campylobacter jejuni is a Gram-negative spiral rod bacterium and is the leading but underreported bacterial food-borne pathogen that causes human campylobacteriosis worldwide. Raw or undercooked poultry products are regarded as a major source for human infection. C. jejuni flagella have been implicated in colonization and adhesion to the mucosal surface of chicken gastrointestinal tracts. Therefore, flagellar proteins would be the excellent targets for further investigation. In this report, we used the recombinant technology to generate a battery of C...
March 2015: Archives of Microbiology
https://www.readbyqxmd.com/read/25404649/manuka-honey-reduces-the-motility-of-pseudomonas-aeruginosa-by-suppression-of-flagella-associated-genes
#11
Aled E L Roberts, Sarah E Maddocks, Rose A Cooper
OBJECTIVES: Manuka honey is a broad-spectrum antimicrobial agent that seems to affect different bacteria in many different ways. It has been shown to be bactericidal against Pseudomonas aeruginosa by destabilizing the cell wall, but we aimed to investigate whether there were further intracellular target sites. METHODS: In this study inhibitory effects of manuka honey on P. aeruginosa were investigated using hydrophobicity assays, two-dimensional electrophoresis, quantitative RT-PCR, transmission electron microscopy and motility assays...
March 2015: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/25289006/the-sensor-kinase-gacs-negatively-regulates-flagellar-formation-and-motility-in-a-biocontrol-bacterium-pseudomonas-chlororaphis-o6
#12
Ji Soo Kim, Yong Hwan Kim, Anne J Anderson, Young Cheol Kim
The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant...
June 2014: Plant Pathology Journal
https://www.readbyqxmd.com/read/25023462/from-molecular-evolution-to-biobricks-and-synthetic-modules-a-lesson-by-the-bacterial-flagellum
#13
REVIEW
Florian Altegoer, Jan Schuhmacher, Patrick Pausch, Gert Bange
The bacterial flagellum is a motility structure and represents one of the most sophisticated nanomachines in the biosphere. Here, we review the current knowledge on the flagellum, its architecture with respect to differences between Gram-negative and Gram-positive bacteria and other species-specific variations (e.g. the flagellar filament protein, Flagellin). We further focus on the mechanism by which the two nucleotide-binding proteins FlhF and FlhG ensure the correct reproduction of flagella place and number (the flagellation pattern)...
October 2014: Biotechnology & Genetic Engineering Reviews
https://www.readbyqxmd.com/read/24655121/regulation-of-bacterial-cell-polarity-by-small-gtpases
#14
Daniela Keilberg, Lotte Søgaard-Andersen
Bacteria are polarized with many proteins localizing dynamically to specific subcellular sites. Two GTPase families have important functions in the regulation of bacterial cell polarity, FlhF homologues and small GTPases of the Ras superfamily. The latter consist of only a G domain and are widespread in bacteria. The rod-shaped Myxococcus xanthus cells have two motility systems, one for gliding and one that depends on type IV pili. The function of both systems hinges on proteins that localize asymmetrically to the cell poles...
April 1, 2014: Biochemistry
https://www.readbyqxmd.com/read/24416296/quorum-sensing-controls-flagellar-morphogenesis-in-burkholderia-glumae
#15
Moon Sun Jang, Eunhye Goo, Jae Hyung An, Jinwoo Kim, Ingyu Hwang
Burkholderia glumae is a motile plant pathogenic bacterium that has multiple polar flagella and one LuxR/LuxI-type quorum sensing (QS) system, TofR/TofI. A QS-dependent transcriptional regulator, QsmR, activates flagellar master regulator flhDC genes. FlhDC subsequently activates flagellar gene expression in B. glumae at 37°C. Here, we confirm that the interplay between QS and temperature is critical for normal polar flagellar morphogenesis in B. glumae. In the wild-type bacterium, flagellar gene expression and flagellar number were greater at 28°C compared to 37°C...
2014: PloS One
https://www.readbyqxmd.com/read/24321074/-preparation-and-characterization-of-monoclonal-antibodies-against-flhf-protein-of-campylobacter-jejuni
#16
Fangzhe Ren, Nan Wang, Yuwei Shang, Xinan Jiao, Jinlin Huang
OBJECTIVE: To develop a prokaryotic expression system for Campylobacrer jejuni FlhF protein and prepare monoclonal antibodies(mAb) against this protein. METHODS: The C. jejuni flhF gene was amplified and inserted into the expression plasmids, pET-30a(+) and pGEX-6p-1. Then the rHis-FlhF protein and rGST-FlhF protein were expressed and purified by affinity column chromatography. They were subsequently used as immunogen and detecting antigen to screen antibodies produced by hybridoma cells against these proteins...
December 2013: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/24102920/the-putative-poc-complex-controls-two-distinct-pseudomonas-aeruginosa-polar-motility-mechanisms
#17
Kimberly N Cowles, Theresa S Moser, Albert Siryaporn, Natsai Nyakudarika, William Dixon, Jonathan J Turner, Zemer Gitai
Each Pseudomonas aeruginosa cell localizes two types of motility structures, a single flagellum and one or two clusters of type IV pili, to the cell poles. Previous studies suggested that these motility structures arrive at the pole through distinct mechanisms. Here we performed a swimming motility screen to identify polar flagellum localization factors and discovered three genes homologous to the TonB/ExbB/ExbD complex that have defects in both flagella-mediated swimming and pilus-mediated twitching motility...
December 2013: Molecular Microbiology
https://www.readbyqxmd.com/read/23600726/spatial-and-numerical-regulation-of-flagellar-biosynthesis-in-polarly-flagellated-bacteria
#18
REVIEW
Barbara I Kazmierczak, David R Hendrixson
Control of surface organelle number and placement is a crucial aspect of the cell biology of many Gram-positive and Gram-negative bacteria, yet mechanistic insights into how bacteria spatially and numerically organize organelles are lacking. Many surface structures and internal complexes are spatially restricted in the bacterial cell (e.g. type IV pili, holdfasts, chemoreceptors), but perhaps none show so many distinct patterns in terms of number and localization as the flagellum. In this review, we discuss two proteins, FlhF and FlhG (also annotated FleN/YlxH), which control aspects of flagellar assembly, placement and number in polar flagellates, and may influence flagellation in some bacteria that produce peritrichous flagella...
May 2013: Molecular Microbiology
https://www.readbyqxmd.com/read/23312848/construction-expression-purification-and-antigenicity-of-recombinant-campylobacter-jejuni-flagellar-proteins
#19
Hung-Yueh Yeh, Kelli L Hiett, John E Line, Brian B Oakley, Bruce S Seal
Campylobacter jejuni, a flagellated, spiral-rod Gram-negative bacterium, is the leading etiologic agent of human acute bacterial gastroenteritis worldwide. The source of this microorganism for human infection has been implicated as consumption and handling of poultry meat where this microorganism is a commensal in the gut. Because the genomes of many C. jejuni isolates have been sequenced, our ultimate goal is to develop protein arrays for exploring this microorganism and host interactions. In this communication, we report cloning, expression and purification of C...
May 6, 2013: Microbiological Research
https://www.readbyqxmd.com/read/23264582/the-gtpase-activity-of-flhf-is-dispensable-for-flagellar-localization-but-not-motility-in-pseudomonas-aeruginosa
#20
Maren Schniederberend, Kholis Abdurachim, Thomas Scott Murray, Barbara I Kazmierczak
The opportunistic human pathogen Pseudomonas aeruginosa uses two surface organelles, flagella and pili, for motility and adhesion in biotic and abiotic environments. Polar flagellar placement and number are influenced by FlhF, which is a signal recognition particle (SRP)-type GTPase. The FlhF proteins of Bacillus subtilis and Campylobacter jejuni were recently shown to have GTPase activity. However, the phenotypes associated with flhF deletion and/or mutation differ between these organisms and P. aeruginosa, making it difficult to generalize a role for FlhF in pseudomonads...
March 2013: Journal of Bacteriology
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