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https://www.readbyqxmd.com/read/28321976/a-5-color-flow-cytometric-method-for-extended-8-part-leukocyte-differential
#1
Julien Guy, Orianne Wagner-Ballon, Olivier Pages, François Bailly, Jessica Borgeot, Marie-C Béné, Marc Maynadié
OBJECTIVES: Microscopic leukocyte differentials display many drawbacks. Several single 5 to 8-color tubes using multiparameter flow cytometry (MFC) are able to provide extended differentials with sequential gating-based analysis strategies. We investigated a new 5-color MFC method to perform an extended 8-part differential with a simplified gating strategy. METHODS: Whole blood was stained with a combination of antibodies including HLA-DR-FITC/CD19-PE/CD45-ECD/CD16-PC5+CD71-PC5/CD5-PC7...
March 21, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28288151/pentoxifylline-modulates-lps-induced-hyperinflammation-in-monocytes-of-preterm-infants-in-vitro
#2
Simone S Schüller, Lukas Wisgrill, Elisabeth Herndl, Andreas Spittler, Elisabeth Förster-Waldl, Kambis Sadeghi, Boris W Kramer, Angelika Berger
BACKGROUND: Pentoxifylline (PTX), a methylxanthine derivate with immunomodulating properties, has been used as adjunctive treatment in severe neonatal sepsis. The aim of the study was to investigate PTX's anti-inflammatory effects on LPS stimulated monocytes of preterm neonates in vitro compared to monocytes from term infants and adult controls. METHODS: Whole cord blood and control adult blood samples were incubated with LPS and PTX. The expression of surface markers, phagocytosis, cytokine secretion and Toll-like-receptor (TLR)4 signaling of monocytes were assessed by flow cytometry...
March 13, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28284665/cryopreservation-and-multipotential-characteristics-evaluation-of-a-novel-type-of-mesenchymal-stem-cells-derived-from-small-tailed-han-sheep-fetal-lung-tissue
#3
Caiyun Ma, Changqing Liu, Xiangchen Li, Taofeng Lu, Chunyu Bai, Yanan Fan, Weijun Guan, Yu Guo
Lung mesenchymal stem cells (L-MSCs) characterized by plasticity, reduced relative immune privilege and high anti-fibrosis characteristics play the crucial role in lung tissue regenerative processes. However, up to date, the multi-differentiation potentials and application values of L-MSCs are still uncertain. In the current study, the Small Tailed Han Sheep embryo L-MSCs line from 12 samples, stocking 124 cryogenically-preserved vials, was successfully established by using primary culture and cell cryopreservation techniques...
March 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/28245371/-expression-of-cd146-in-adult-and-children-s-acute-b-cell-lymphoblastic-leukemia-and-its-significance
#4
Xiao-Qing Xie, Wei-Min Wang, Si-Lin Gan, Sheng-Mei Chen, Qiu-Tang Zhang, Xin-Sheng Xie, Yan-Fang Liu, Yuan-Dong Cheng, Yu-Feng Liu, Xue-Ju Xu, Hui Sun
OBJECTIVE: To explore the differences of CD146 expression in adult and children's acute B cell lymphoblastic leukemia(B-ALL), and its relation with clinical features, molecular biological and cytogenctic claracteristics. METHODS: The expression of CD146 in bone marrow samples from adult and children's B-ALL patients were detected by flow cytometry (FCM) and the relation of CD146 abnormal high expression with the patients' clinical features, molecular biological and cytogenetical characteristics, as well as other antigens were analyzed...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28239788/expression-of-surface-molecules-in-human-mesenchymal-stromal-cells-co-cultured-with-nucleated-umbilical-cord-blood-cells
#5
Yu A Romanov, E E Balashova, N E Volgina, N V Kabaeva, T N Dugina, G T Sukhikh
We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell-cell contacts with leukocytes and only for bone marrow derived cells...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28165036/the-promotion-of-erythropoiesis-via-the-regulation-of-reactive-oxygen-species-by-lactic-acid
#6
Shun-Tao Luo, Dong-Mei Zhang, Qing Qin, Lian Lu, Min Luo, Fu-Chun Guo, Hua-Shan Shi, Li Jiang, Bin Shao, Meng Li, Han-Shuo Yang, Yu-Quan Wei
The simultaneous increases in blood lactic acid and erythrocytes after intense exercise could suggest a link between lactate and the erythropoiesis. However, the effects of lactic acid on erythropoiesis remain to be elucidated. Here, we utilized a mouse model to determine the role of lactic acid in this process in parallel with studies using leukaemic K562 cells. Treatment of K562 cells in vitro with lactic acid increased the mRNA and protein expression of haemoglobin genes and the frequency of GPA(+) cells...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28134816/%C3%AE-6-integrin-%C3%AE-6-high-transferrin-receptor-cd71-low-keratinocyte-stem-cells-are-more-potent-for-generating-reconstructed-skin-epidermis-than-rapid-adherent-cells
#7
Elodie Metral, Nicolas Bechetoille, Frédéric Demarne, Walid Rachidi, Odile Damour
The epidermis basal layer is composed of two keratinocyte populations: Keratinocyte Stem cells (KSC) and Transitory Amplifying (TA) cells that arise from KSC division. Unfortunately, no specific marker exists to differ between KSC and TA cells. Here, we aimed at comparing two different methods that pretended to isolate these two populations: (i) the rapid adhesion method on coated substrate and (ii) the flow cytometry method, which is based on the difference in cell surface expressions of the α6 integrin and transferrin receptor (CD71)...
January 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28069109/immunomodulatory-effects-of-bone-marrow-mesenchymal-stem-cells-overexpressing-heme-oxygenase-1-protective-effects-on-acute-rejection-following-reduced-size-liver-transplantation-in-a-rat-model
#8
Zhong-Yang Shen, Bin Wu, Tao Liu, Yang Yang, Ming-Li Yin, Wei-Ping Zheng, Bo-Ya Zhang, Hong-Li Song
Here we explore the T-lymphocyte suppressive and immunomodulatory effects of bone marrow mesenchymal stem cells (BMMSCs) overexpressing heme oxygenase-1 (HO-1) on acute rejection following reduced-size liver transplantation (RLT) in a rat model. The proliferation activity, cell cycle progression, secretion of proinflammatory cytokines, expression of CD25 and CD71 in lymphocytes, and activity of NK cells were found to be significantly lowered, and the proportion of regulatory T cells (Tregs) was found to be increased relative to BMMSCs when Adv-HO-1/BMMSCs were co-cultured with Con A ex vivo; secretion of anti-inflammatory cytokines was significantly higher...
March 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28068330/loss-of-p53-induces-leukemic-transformation-in-a-murine-model-of-jak2-v617f-driven-polycythemia-vera
#9
T Tsuruta-Kishino, J Koya, K Kataoka, K Narukawa, Y Sumitomo, H Kobayashi, T Sato, M Kurokawa
As leukemic transformation of myeloproliferative neoplasms (MPNs) worsens the clinical outcome, reducing the inherent risk of the critical event in MPN cases could be beneficial. Among genetic alterations concerning the transformation, the frequent one is TP53 mutation. Here we show that retroviral overexpression of Jak2 V617F mutant into wild-type p53 murine bone marrow cells induced polycythemia vera (PV) in the recipient mice, whereas Jak2 V617F-transduced p53-null mice developed lethal leukemia after the preceding PV phase...
January 9, 2017: Oncogene
https://www.readbyqxmd.com/read/28062363/governing-roles-for-trib3-pseudokinase-during-stress-erythropoiesis
#10
Arvind Dev, Ruth Asch, Edward Jachimowicz, Nicole Rainville, Ashley Johnson, Emily Greenfest-Allen, Don M Wojchowski
In response to anemia, the heightened production of erythropoietin (EPO) can sharply promote erythroid progenitor cell (EPC) formation. Specific mediators of such EPO- accelerated erythropoiesis, however, are not well understood. Presently, we first report that the expression of Trib3 in adult bone marrow EPCs in vivo is nominal at steady state, but strongly activated on EPO challenge. In a knockout mouse model, Trib3 disruption modestly increased steady-state erythrocyte numbers and decreased mean corpuscular volume...
January 4, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/27974744/notch-regulates-th17-differentiation-and-controls-trafficking-of-il-17-and-metabolic-regulators-within-th17-cells-in-a-context-dependent-manner
#11
Manuel Coutaz, Benjamin P Hurrell, Floriane Auderset, Haiping Wang, Stefanie Siegert, Gerard Eberl, Ping-Chih Ho, Freddy Radtke, Fabienne Tacchini-Cottier
Th17 cells play critical roles in host defense and autoimmunity. Emerging data support a role for Notch signaling in Th17 cell differentiation but whether it is a positive or negative regulator remains unclear. We report here that T cell-specific deletion of Notch receptors enhances Th17 cell differentiation in the gut, with a corresponding increase in IL-17 secretion. An increase in Th17 cell frequency was similarly observed following immunization of T cell specific Notch mutant mice with OVA/CFA. However, in this setting, Th17 cytokine secretion was impaired, and increased intracellular retention of IL-17 was observed...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27931817/evaluation-of-the-pigret-assay-in-rats-by-single-oral-dosing-with-azidothymidine
#12
Hisakazu Sanada, Tomoka Ohsumi, Naomi Koyama, Taishi Miyashita, Kazuto Hashimoto
In vivo phosphatidylinositol glycan, class A (Pig-a) gene mutation assay using peripheral blood is known to be a novel and useful tool to evaluate the mutagenicity of compounds. Recently, the rat PIGRET assay which is an improved method for measuring Pig-a mutant cells in reticulocytes with magnetic enrichment of CD71 positive cells has been developed. Several reports showed that the PIGRET assay could detect the increase of Pig-a mutant frequency earlier than the Pig-a assay in total red blood cells (RBC Pig-a assay)...
November 15, 2016: Mutation Research
https://www.readbyqxmd.com/read/27931806/detection-of-pig-a-gene-mutants-in-rat-peripheral-blood-following-a-single-urethane-treatment-a-comparison-of-the-rbc-pig-a-and-pigret-assays
#13
Kazunori Narumi, Yohei Fujiishi, Emiko Okada, Wakako Ohyama
The rat red blood cell (RBC) Pig-a assay has been recommended by an expert working group of the International Workshop on Genotoxicity Testing as a potential new method to evaluate in vivo gene mutations in regulatory genotoxicity risk assessments. In a collaborative study in Japan, an improved Pig-a assay using reticulocytes (PIGRET assay) with magnetic enrichment of CD71-positive cells was evaluated, and it was revealed that this assay could detect the mutagenicity of chemicals earlier than the RBC Pig-a assay could...
November 15, 2016: Mutation Research
https://www.readbyqxmd.com/read/27930538/binocular-indirect-ophthalmo-microscope-assistant-gas-perfused-pars-plana-vitrectomy-a-novel-technique-for-vitreous-sample-acquisition
#14
Luyi Zhang, Xiaoli Yang, Qingqing Zheng, Miaoqin Wu
The vitreous sample has been used for the diagnosis of uveitis and intraocular malignancy for decades. The sample volume is usually limited to 1 mL with current techniques. In the present study, a novel technique for higher amount of vitreous sample acquisition, that is, Binocular Indirect Ophthalmo Microscope-assistant gas-perfused pars plana vitrectomy (BAG-PPV) was invented.For diagnostic purpose, BAG-PPV with 23-ga vitrectomy system was performed on a 54-year-old Chinese male with the symptom of bilateral atypical uveitis...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27902785/apoptosis-related-gene-expression-profiling-in-hematopoietic-cell-fractions-of-mds-patients
#15
Saskia Mc Langemeijer, Niccolo Mariani, Ruth Knops, Christian Gilissen, Rob Woestenenk, Theo de Witte, Gerwin Huls, Bert A van der Reijden, Joop H Jansen
Although the vast majority of patients with a myelodysplastic syndrome (MDS) suffer from cytopenias, the bone marrow is usually normocellular or hypercellular. Apoptosis of hematopoietic cells in the bone marrow has been implicated in this phenomenon. However, in MDS it remains only partially elucidated which genes are involved in this process and which hematopoietic cells are mainly affected. We employed sensitive real-time PCR technology to study 93 apoptosis-related genes and gene families in sorted immature CD34+ and the differentiating erythroid (CD71+) and monomyeloid (CD13/33+) bone marrow cells...
2016: PloS One
https://www.readbyqxmd.com/read/27870894/identification-of-stages-of-erythroid-differentiation-in-bone-marrow-and-erythrocyte-subpopulations-in-blood-circulation-that-are-preferentially-lost-in-autoimmune-hemolytic-anemia-in-mouse
#16
Sreoshi Chatterjee, Nitin Bhardwaj, Rajiv K Saxena
Repeated weekly injections of rat erythrocytes produced autoimmune hemolytic anemia (AIHA) in C57BL/6 mice after 5-6 weeks. Using the double in vivo biotinylation (DIB) technique, recently developed in our laboratory, turnover of erythrocyte cohorts of different age groups during AIHA was monitored. Results indicate a significant decline in the proportion of reticulocytes, young and intermediate age groups of erythrocytes, but a significant increase in the proportion of old erythrocytes in blood circulation...
2016: PloS One
https://www.readbyqxmd.com/read/27758818/immunophenotypic-analysis-of-erythroid-dysplasia-in-myelodysplastic-syndromes-a-report-from-the-imdsflow-working-group
#17
Theresia M Westers, Eline M P Cremers, Uta Oelschlaegel, Ulrika Johansson, Peter Bettelheim, Sergio Matarraz, Alberto Orfao, Bijan Moshaver, Lisa Eidenschink Brodersen, Michael R Loken, Denise A Wells, Dolores Subirá, Matthew Cullen, Jeroen G Te Marvelde, Vincent H J van der Velden, Frank W M B Preijers, Sung-Chao Chu, Jean Feuillard, Estelle Guérin, Katherina Psarra, Anna Porwit, Leonie Saft, Robin Ireland, Timothy Milne, Marie C Béné, Birgit I Witte, Matteo G Della Porta, Wolfgang Kern, Arjan A van de Loosdrecht
Current recommendations for diagnosing myelodysplastic syndromes endorse flow cytometry as an informative tool. Most flow cytometry protocols focus on the analysis of progenitor cells and the evaluation of the maturing myelomonocytic lineage. However, one of the most frequently observed features of myelodysplastic syndromes is anemia, which may be associated with dyserythropoiesis. Therefore, analysis of changes in flow cytometry features of nucleated erythroid cells may complement current flow cytometry tools...
February 2017: Haematologica
https://www.readbyqxmd.com/read/27736268/iron-chelation-an-adjuvant-therapy-to-target-metabolism-growth-and-survival-of-murine-pten-deficient-t-lymphoma-and-human-t-lymphoblastic-leukemia-lymphoma
#18
Joy Benadiba, Celia Rosilio, Marielle Nebout, Vera Heimeroth, Zouhour Neffati, Alexandra Popa, Didier Mary, Emmanuel Griessinger, Véronique Imbert, Nicolas Sirvent, Jean-François Peyron
Iron is an essential nutrient, acting as a catalyst for metabolic reactions that are fundamental to cell survival and proliferation. Iron complexed to transferrin is delivered to the metabolism after endocytosis via the CD71 surface receptor. We found that transformed cells from a murine PTEN-deficient T-cell lymphoma model and from T-cell acute lymphoblastic leukemia/lymphoma (T-ALL/T-LL) cell lines overexpress CD71. As a consequence, the cells developed an addiction toward iron whose chelation by deferoxamine (DFO) dramatically affected their survival to induce apoptosis...
October 13, 2016: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/27733786/identification-and-characterization-of-pig-adipose-derived-progenitor-cells
#19
Shuang Zhang, Chunyu Bai, Dong Zheng, Yuhua Gao, Yanan Fan, Lu Li, Weijun Guan, Yuehui Ma
Adipose-derived stem cells (ADSCs) are multipotent, and can be differentiated into many cell types in vitro. In this study, tissues from pigs were chosen to identify and characterize ADSCs. Primary ADSCs were sub-cultured to passage 28. The surface markers of ADSCs: CD29, CD71, CD73, CD90, and CD166 were detected by reverse-transcription polymerase chain reaction assays and the markers CD29, CD44, CD105, and vimentin were detected by immunofluorescence. Growth curves and the capacity of clone-forming were performed to test the proliferation of ADSCs...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27725143/analysis-of-hematopathology-and-alteration-of-jak1-stat3-stat5-signaling-axis-in-experimental-myelodysplastic-syndrome
#20
Suchismita Daw, Ritam Chatterjee, Aditya Law, Sujata Law
Hematological disorders like myelodysplastic syndrome (MDS) may arise due to cumulative dysregulation of various signalling pathways controlling proliferation, differentiation, maturation and apoptosis of bone marrow cells. This devastating bone marrow condition can be due to consequential abnormalities in haematopoiesis as well as its supportive microenvironment. Although mutations related to JAK/STAT pathway are common in myeloproliferative neoplasms, further studies are required to fully explore the myelodysplastic scenario regarding the concerned pathway...
December 25, 2016: Chemico-biological Interactions
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