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Recovery and adaptation

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https://www.readbyqxmd.com/read/29049322/pgc-1%C3%AE-and-exercise-intensity-dependent-adaptations-in-mouse-skeletal-muscle
#1
Nina Brandt, Maja Munk Dethlefsen, Jens Bangsbo, Henriette Pilegaard
The aim of the present study was to examine the role of PGC-1α in intensity dependent exercise and exercise training-induced metabolic adaptations in mouse skeletal muscle. Whole body PGC-1α knockout (KO) and littermate wildtype (WT) mice performed a single treadmill running bout at either low intensity (LI) for 40 min or moderate intensity (MI) for 20 min. Blood and quadriceps muscles were removed either immediately after exercise or at 3h or 6h into recovery from exercise and from resting controls. In addition PGC-1α KO and littermate WT mice were exercise trained at either low intensity (LIT) for 40 min or at moderate intensity (MIT) for 20 min 2 times pr...
2017: PloS One
https://www.readbyqxmd.com/read/29048506/the-comfort-ability-pain-management-workshop-a-preliminary-nonrandomized-investigation-of-a-brief-cognitive-biobehavioral-and-parent-training-intervention-for-pediatric-chronic-pain
#2
Rachael Coakley, Tessa Wihak, Joe Kossowsky, Christina Iversen, Carolina Donado
Objective: Psychological intervention is widely recognized as an integral part of the recovery process from pediatric chronic pain, but service acquisition is often limited by resource barriers. The aim of this study was to assess the feasibility, acceptability, and satisfaction of a brief, structured, skills-based, group intervention designed expressly to address gaps in service delivery. Exploratory outcomes were also assessed. Method: Adolescents with chronic pain (n = 102; ages 10-17 years) and their mothers (n = 105) completed self-report questionnaires at baseline, 1-week, 1-month, and 3-month posttreatment...
September 18, 2017: Journal of Pediatric Psychology
https://www.readbyqxmd.com/read/29047828/three-dimensional-surface-microtopography-recovery-from-a-multifocus-image-sequence-using-an-omnidirectional-modified-laplacian-operator-with-adaptive-window-size
#3
Yingzhong Tian, Huijuan Hu, Haoyang Cui, Shouchen Yang, Ji Qi, Zhiming Xu, Long Li
Optical microscopy enables the observation of highly magnified objects and material structures on microsurfaces, but it can only acquire 2D images. In order to observe areal features more accurately and intuitively, 3D surface microtopography recovery has been applied to form a 3D surface model of an object from its 2D image sequence. In the 3D reconstruction of the focus evaluation operator, we have the gray variance operator, the gray-scale difference absolute sum operator, the Roberts gradient operator, the Tenengrad gradient operator, the improved Laplace operator, etc...
August 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/29046968/development-of-an-elution-device-for-virocap-virus-filters
#4
Christine Susan Fagnant, Matthew Toles, Nicolette Angela Zhou, Jacob Powell, John Adolphsen, Yifei Guan, Byron Ockerman, Jeffry Hiroshi Shirai, David S Boyle, Igor Novosselov, John Scott Meschke
Environmental surveillance of waterborne pathogens is vital for monitoring the spread of diseases, and electropositive filters are frequently used for sampling wastewater and wastewater-impacted surface water. Viruses adsorbed to electropositive filters require elution prior to detection or quantification. Elution is typically facilitated by a peristaltic pump, although this requires a significant startup cost and does not include biosafety or cross-contamination considerations. These factors may pose a barrier for low-resource laboratories that aim to conduct environmental surveillance of viruses...
October 19, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29041379/surface-deformation-recovery-algorithm-for-reflector-antennas-based-on-geometric-optics
#5
Jianhui Huang, Huiliang Jin, Qian Ye, Guoxiang Meng
Surface deformations of large reflector antennas highly depend on elevation angle. This paper adopted a scheme with the ability to conduct measurement at any elevation angle: carrying an emission source, an unmanned aerial vehicle (UAV) scans the antenna on a near-field plane, meanwhile the antenna stays stationary. Near-field amplitude is measured in the scheme. To recover the deformation from the measured amplitude, this paper proposed a novel algorithm by deriving the deformation-amplitude equation, which reveals the relation between the surface deformation and the near-field amplitude...
October 2, 2017: Optics Express
https://www.readbyqxmd.com/read/29040771/contrasting-ecophysiological-strategies-related-to-drought-the-case-of-a-mixed-stand-of-scots-pine-pinus-sylvestris-and-a-submediterranean-oak-quercus-subpyrenaica
#6
Paula Martín-Gómez, Mònica Aguilera, Jesús Pemán, Eustaquio Gil-Pelegrín, Juan Pedro Ferrio
Submediterranean forests are considered an ecotone between Mediterranean and Eurosiberian ecosystems, and are very sensitive to global change. A decline of Scots pine (Pinus sylvestris L.) and a related expansion of oak species (Quercus spp.) have been reported in the Spanish Pre-Pyrenees. Although this has been associated with increasing drought stress, the underlying mechanisms are not fully understood, and suitable monitoring protocols are lacking. The aim of this study is to bring insight into the physiological mechanisms anticipating selective decline of the pines, with particular focus on carbon and water relations...
October 13, 2017: Tree Physiology
https://www.readbyqxmd.com/read/29037326/%C3%AE-hydroxy-%C3%AE-methylbutyrate-free-acid-supplementation-may-improve-recovery-and-muscle-adaptations-after-resistance-training-a-systematic-review
#7
REVIEW
Vagner R Silva, Felipe L Belozo, Thayana O Micheletti, Marcelo Conrado, Jeffrey R Stout, Gustavo D Pimentel, Adam M Gonzalez
β-Hydroxy-β-methylbutyrate free acid (HMB-FA) has been suggested to accelerate the regenerative capacity of skeletal muscle after high-intensity exercise and attenuate markers of skeletal muscle damage. Herein a systematic review on the use of HMB-FA supplementation as an ergogenic aid to improve measures of muscle recovery, performance, and hypertrophy after resistance training was conducted. This review was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses. We included randomized, double-blinded, placebo-controlled trials investigating the effects of HMB-FA supplementation in conjunction with resistance exercise in humans...
September 2017: Nutrition Research
https://www.readbyqxmd.com/read/29034483/effect-of-sensory-and-motor-connectivity-on-hand-function-in-pediatric-hemiplegia
#8
Disha Gupta, Alexandre Barachant, Andrew M Gordon, Claudio Ferre, Hsing-Ching Kuo, Jason B Carmel, Kathleen M Friel
OBJECTIVE: We tested the hypothesis that somatosensory system injury would more strongly affect movement than motor system injury in children with unilateral cerebral palsy (USCP). This hypothesis was based on how somatosensory and corticospinal circuits adapt to injury during development: while the motor system can maintain connections to the impaired hand from the uninjured hemisphere, this doesn't occur in the somatosensory system. As a corollary, cortical injury strongly impairs sensory function, so we hypothesized that cortical lesions would impair hand function more than subcortical lesions...
October 16, 2017: Annals of Neurology
https://www.readbyqxmd.com/read/29033970/spermine-regulates-pollen-tube-growth-by-modulating-ca-2-dependent-actin-organization-and-cell-wall-structure
#9
Iris Aloisi, Giampiero Cai, Claudia Faleri, Lorella Navazio, Donatella Serafini-Fracassini, Stefano Del Duca
Proper growth of the pollen tube depends on an elaborate mechanism that integrates several molecular and cytological sub-processes and ensures a cell shape adapted to the transport of gametes. This growth mechanism is controlled by several molecules among which cytoplasmic and apoplastic polyamines. Spermine (Spm) has been correlated with various physiological processes in pollen, including structuring of the cell wall and modulation of protein (mainly cytoskeletal) assembly. In this work, the effects of Spm on the growth of pear pollen tubes were analyzed...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29032171/reprint-of-state-of-the-art-on-ultrasonic-oil-production-technique-for-eor-in-china
#10
Zhenjun Wang, Congbo Yin
Ultrasonic oil production technique for enhanced oil recovery (EOR) attracts more attention due to its high adaptability, simple operation, low cost and zero pollution to the oil reservoir. In this paper, recent new downhole tools used for enhanced oil recovery developed in China are summarized. Furthermore, research advance on some key problems that affect the widespread application of ultrasonic oil production technique in China are also summarized in view of what are the primary factors that influence crude oil paraffin inhibition and viscosity reduction, whether ultrasonic excitation is better than chemical agent for any plugs removal and whether ultrasound-chemical combination plug removal technology has the best plugs removal effect...
October 7, 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29030957/mollusc-shells-as-metagenomic-archives-the-true-treasure-is-the-chest-itself
#11
Marie-Agnès Coutellec
Mollusc shells, beyond the treasure of information inherently conveyed through their morphology and chemical composition also have the capacity to preserve DNA sequences over the long term in their inner structure. This has been clearly demonstrated for the first time in the study published in this issue of Molecular Ecology Resources by Der Sarkissian et al. (). With a methodology specifically dedicated to ancient DNA and solid matrices, the authors were able to successfully extract and amplify DNA from marine shells spanning the last 7,000 years...
September 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29028400/label-free-study-of-cosmonaut-s-urinary-proteome-changes-after-long-duration-spaceflights
#12
A Brzhozovskiy, A Kononikhin, M Indeykina, LKh Pastushkova, I A Popov, E N Nikolaev, I M Larina
During the entire time that cosmonauts stay on board the international space station, different extreme space flight factors affect their bodies. In order to find out what physiological changes occur under the influence of spaceflight, different parameters of the human body before and after flights are monitored. Analysis of the urine proteome is one of the most perspective non-invasive methods of condition monitoring. The aim of the study was to perform a comparative semi-quantitative label-free urine proteome analysis of samples collected from 21 cosmonauts before and after long-duration spaceflight at the international space station...
August 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29027041/increased-hepatic-fatty-acid-polyunsaturation-precedes-ectopic-lipid-deposition-in-the-liver-in-adaptation-to-high-fat-diets-in-mice
#13
Ana Francisca Soares, João M N Duarte, Rolf Gruetter
OBJECTIVE: We monitored hepatic lipid content (HLC) and fatty acid (FA) composition in the context of enhanced lipid handling induced by a metabolic high-fat diet (HFD) challenge and fasting. MATERIALS AND METHODS: Mice received a control diet (10% of kilocalories from fat, N = 14) or an HFD (45% or 60% of kilocalories from fat, N = 10 and N = 16, respectively) for 26 weeks. A subset of five mice receiving an HFD (60% of kilocalories from fat) were switched to the control diet for the final 7 weeks...
October 12, 2017: Magma
https://www.readbyqxmd.com/read/29026944/improving-elution-strategies-for-chelex%C3%A2-dgt-passive-samplers
#14
Delphine Devillers, Rémy Buzier, Adeline Charriau, Gilles Guibaud
Elution of Chelex® binding layers, commonly used for the diffusive gradients in thin films technique (DGT), is recognized as the most important contributor to the uncertainty of DGT measurements. Limiting uncertainty requires the use of optimized procedures and suitable elution recoveries (f e ). This work therefore investigated elution robustness to propose improved strategies. A wide range of conditions were investigated for the main elution parameters (Chelex® particle size, elution time, Chelex® loading, and eluent concentration and volume) on Al(III), Cd(II), Co(II), Cr(III), Cu(II), Ni(II), Pb(II), and Zn(II)...
October 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29026113/highly-efficient-cellular-cloning-using-ferro-core-micropallet-arrays
#15
Trisha M Westerhof, Wesley A Cox-Muranami, Guann-Pyng Li, Mark Bachman, Hung Fan, Edward L Nelson
Advancing knowledge of biological mechanisms has come to depend upon genetic manipulation of cells and organisms, relying upon cellular cloning methods that remain unchanged for decades, are labor and time intensive, often taking many months to come to fruition. Thus, there is a pressing need for more efficient processes. We have adapted a newly developed micropallet array platform, termed the "ferro-core micropallet array", to dramatically improve and accelerate the process of isolating clonal populations of adherent cells from heterogeneous mixtures retaining the flexibility of employing a wide range of cytometric parameters for identifying colonies and cells of interest...
October 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29025926/enhancing-spinal-plasticity-amplifies-the-benefits-of-rehabilitative-training-and-improves-recovery-from-stroke
#16
Anna M Wiersma, Karim Fouad, Ian R Winship
The limited recovery that occurs following stroke happens almost entirely in the first weeks post injury. Moreover, the efficacy of rehabilitative training is limited beyond this narrow time frame. Sprouting of spared corticospinal tract axons in the contralesional spinal cord makes a significant contribution to sensorimotor recovery, but this structural plasticity is also limited to the first few weeks after stroke. Here, we tested the hypothesis that inducing plasticity in the spinal cord during chronic stroke could improve recovery from persistent sensorimotor impairment...
October 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29025427/adaptive-hybrid-robotic-system-for-rehabilitation-of-reaching-movement-after-a-brain-injury-a-usability-study
#17
F Resquín, J Gonzalez-Vargas, J Ibáñez, F Brunetti, I Dimbwadyo, L Carrasco, S Alves, C Gonzalez-Alted, A Gomez-Blanco, J L Pons
BACKGROUND: Brain injury survivors often present upper-limb motor impairment affecting the execution of functional activities such as reaching. A currently active research line seeking to maximize upper-limb motor recovery after a brain injury, deals with the combined use of functional electrical stimulation (FES) and mechanical supporting devices, in what has been previously termed hybrid robotic systems. This study evaluates from the technical and clinical perspectives the usability of an integrated hybrid robotic system for the rehabilitation of upper-limb reaching movements after a brain lesion affecting the motor function...
October 12, 2017: Journal of Neuroengineering and Rehabilitation
https://www.readbyqxmd.com/read/29022124/faster-photosynthetic-induction-in-tobacco-by-expressing-cyanobacterial-flavodiiron-proteins-in-chloroplasts
#18
Rodrigo Gómez, Néstor Carrillo, María P Morelli, Suresh Tula, Fahimeh Shahinnia, Mohammad-Reza Hajirezaei, Anabella F Lodeyro
Plants grown in the field experience sharp changes in irradiation due to shading effects caused by clouds, other leaves, etc. The excess of absorbed light energy is dissipated by a number of mechanisms including cyclic electron transport, photorespiration, and Mehler-type reactions. This protection is essential for survival but decreases photosynthetic efficiency. All phototrophs except angiosperms harbor flavodiiron proteins (Flvs) which relieve the excess of excitation energy on the photosynthetic electron transport chain by reducing oxygen directly to water...
October 11, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/29017960/upper-respiratory-tract-nociceptor-stimulation-and-stress-response-following-acute-and-repeated-cyfluthrin-inhalation-in-normal-and-pregnant-rats-physiological-rat-specific-adaptions-can-easily-be-misunderstood-as-adversities
#19
Juergen Pauluhn
This paper reviews the results from past regulatory and mechanistic inhalation studies in rats with the type II pyrethroid Cyfluthrin. Apart from many chemical irritants, Cyfluthrin was shown to be a neuroexcitatory agent without any inherent tissue-destructive or irritant property. Thus, any Cyfluthrin-induced neuroexcitatory afferent sensory stimulus from peripheral nociceptors in the upper respiratory tract is likely to be perceived as a transient stimulus triggering annoyance and/or avoidance by both rats and humans...
October 7, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28994750/use-of-immunolabeling-to-analyze-stable-dynamic-and-nascent-microtubules-in-the-zebrafish-embryo
#20
Rebecca J McFarland, Sharlene P Brown, Eudorah Vital, Jonathan M Werner, Rachel M Brewster
Microtubules (MTs) are dynamic and fragile structures that are challenging to image in vivo, particularly in vertebrate embryos. Immunolabeling methods are described here to analyze distinct populations of MTs in the developing neural tube of the zebrafish embryo. While the focus is on neural tissue, this methodology is broadly applicable to other tissues. The procedures are optimized for early to mid-somitogenesis-stage embryos (1 somite to 12 somites), however they can be adapted to a range of other stages with relatively minor adjustments...
September 20, 2017: Journal of Visualized Experiments: JoVE
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