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https://www.readbyqxmd.com/read/28622602/assessment-of-the-phenolic-profile-antimicrobial-activity-and-oxidative-stability-of-transgenic-perilla-frutescens-l-overexpressing-tocopherol-methyltransferase-%C3%AE-tmt-gene
#1
Bimal Kumar Ghimire, Chang Yeon Yu, Ill-Min Chung
This study evaluated the effects of enhanced concentrations of α-tocopherol and phenolic compounds on the resistance and stability of Perilla oil in transgenic Perilla frutescens plants against various tested pathogenic bacteria by over-expressing the γ-tmt gene. The concentration of phenolic compounds in the non-transgenic samples was 9313.198 ± 18.887 μg g(-1) dry weight (DW), whereas the total concentration of the transgenic samples ranged from 9118.015 ± 18.822 to 10527.612 ± 20.411 μg g(-1) DW...
June 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28602194/online-coupling-of-fully-automatic-in-syringe-dispersive-liquid-liquid-microextraction-with-oxidative-back-extraction-to-inductively-coupled-plasma-spectrometry-for-sample-clean-up-in-elemental-analysis-a-proof-of-concept
#2
Burkhard Horstkotte, Kateřina Fikarová, David J Cocovi-Solberg, Hana Sklenářová, Petr Solich, Manuel Miró
A proof of concept of a novel automatic sample cleanup approach for metal assays in troublesome matrixes as a front-end sample pre-treatment to inductively coupled plasma optical emission spectroscopy - ICP-OES - is herein presented. Target metals, namely, copper, lead, and cadmium were complexed in-system quantitatively using ammonium pyrrolidine dithiocarbamate (APDC) and transferred into a minute volume of toluene as extractant employing lab-in-syringe magnetic stirring-assisted dispersive liquid-liquid microextraction (LIS-MSA-DLLME)...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28581735/covalent-capture-of-aligned-self-assembling-nanofibers
#3
I-Che Li, Jeffrey D Hartgerink
A great deal of effort has been invested in the design and characterization of systems which spontaneously assemble into nanofibers. These systems are interesting for their fundamental supramolecular chemistry and have also been shown to be promising materials, particularly for biomedical applications. Multidomain peptides are one such assembler, and in previous work we have demonstrated the reversibility of their assembly under mild and easily controlled conditions, along with their utility for time-controlled drug delivery, protein delivery, cell encapsulation, and cell delivery applications...
June 14, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28578863/estimating-the-cascade-of-hepatitis-c-testing-care-and-treatment-among-people-who-inject-drugs-in-australia
#4
Jenny Iversen, Jason Grebely, Beth Catlett, Philip Cunningham, Gregory J Dore, Lisa Maher
BACKGROUND: Hepatitis C virus (HCV) infection is endemic among people who inject drugs (PWID) globally. Despite high prevalence, treatment uptake is low, with cumulative uptake <10% in most settings. This study aimed to populate the cascade of HCV testing, care and treatment among PWID using data collected in Australia prior to the introduction of broadly accessible interferon-free direct-acting antiviral (DAA) therapies in March 2016. METHODS: The Australian Needle and Syringe Program Survey is a cross-sectional surveillance system that recruits ∼2300 PWID annually and collects behavioural data and dried blood samples (DBS)...
June 1, 2017: International Journal on Drug Policy
https://www.readbyqxmd.com/read/28577265/on-the-injectability-of-free-standing-magnetic-nanofilms
#5
Silvia Taccola, Virginia Pensabene, Toshinori Fujie, Shinji Takeoka, Nicola M Pugno, Virgilio Mattoli
Free-standing films with sub-micrometric thickness, composed of soft polymers and functional nanostructures are promising candidates for many potential applications in the biomedical field, such as reduced port abdominal surgery. In this work, freely suspended poly(L-lactic acid) nanofilms with controlled morphology embedding superparamagnetic iron oxide nanoparticles were fabricated by spin-coating deposition. The mechanical properties of magnetic nanofilms were investigated by Strain-Induced Elastic Buckling Instability for Mechanical Measurements (SIEBIMM) test...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28574197/cross-sectional-study-of-the-microbiological-safety-profile-of-reusing-hyaluronic-acid-fillers
#6
Rubens Pontello, Rogério Nabor Kondo, Priscila Daiane Pavezzi, Theo Nicolacopulos, Julia Pagung Kippert, Clarissa Lena
INTRODUCTION: Facial filling with hyaluronic acid (HA) is a dermatological procedure that has been emerging today. There are not many references regarding safety of reusing the remaining product for later touch-up in the same patient. OBJECTIVE: To determine the microbiological safety of reusing hyaluronic acid that is remnant from syringes used for facial filling, stored at room temperature or cooled in a refrigerator at 4°C. MATERIALS AND METHODS: In culture medium, small aliquots of leftovers from 31 hyaluronic acid fillers, previously used for facial filling, were inoculated...
June 2, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28567622/fungal-pretreatment-of-sweet-sorghum-bagasse-with-supplements-improvement-in-lignin-degradation-selectivity-and-enzymatic-saccharification
#7
Vartika Mishra, Asim K Jana, Mithu Maiti Jana, Antriksh Gupta
Sweet sorghum bagasse (SSB) from food processing and agricultural industry has attracted the attention for uses in production of biofuel, enzymes and other products. The alteration in lignocellulolytic enzymes by use of supplements in fungal pretreatment of SSB to achieve higher lignin degradation, selectivity value and enzymatic hydrolysis to fermentable sugar was studied. Fungal strain Coriolus versicolor was selected for pretreatment due to high ligninolytic and low cellulolytic enzyme production resulting in high lignin degradation and selectivity value...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28551141/novel-approach-to-two-component-speciation-analysis-spectrophotometric-flow-based-determinations-of-fe-ii-fe-iii-and-cr-iii-cr-vi
#8
J Paluch, J Kozak, M Wieczorek, M Kozak, J Kochana, K Widurek, M Konieczna, P Kościelniak
The proposed approach to two-component speciation analysis relies on simultaneous application of two calibration methods to determination of two different forms of an analyte. One form is determined in extrapolative way, whereas the second form is determined in interpolative way, with the use of the same calibration graph, after appropriate chemical treatment, e.g. oxidation or reduction. The applicability of the approach has been verified on the examples of spectrophotometric determinations of Fe(II)/Fe(III) and Cr(VI)/Cr(III) using 1,10-phenanthroline and 1,5-diphenylcarbazide methods, respectively...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28540845/simple-and-rapid-dual-dispersive-liquid-liquid-microextraction-as-an-innovative-extraction-method-for-uranium-in-real-water-samples-prior-to-the-determination-of-uranium-by-a-spectrophotometric-technique
#9
Naeemullah Khan, Mustafa Tuzen, Tasneem Gul Kazi
An innovative, rapid, and simple dual-dispersive liquidߛliquid microextraction (DDLL-ME) approach was used to extract uranium from real samples for the first time. The main objective of this study was to disperse extraction solvent by using an air-agitated syringe system to overcome matrix effects and avoid dispersion of hazardous dispersive organic solvents by using heat. The DDLL-ME method consisted of two dispersive liquidߛrliquid extraction steps with chloroform as the extracting solvent. Uranium formed complexes with 4-(2-thiazolylazo) resorcinol in the aqueous phase and was extracted in extracting solvent (chloroform) after the first dispersive liquidߛrliquid process...
May 25, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28531830/isotope-coded-derivatization-based-lc-esi-ms-ms-methods-using-a-pair-of-novel-reagents-for-quantification-of-hydroxycinnamic-acids-and-hydroxybenzoic-acids-in-fermented-brown-rice-product
#10
Shoujiro Ogawa, Kiriko Takafuji, Sumi Tsubuku, Yukiko Horie, Shigeo Ikegawa, Tatsuya Higashi
Hydroxycinnamic acids (HCAs) and hydroxybenzoic acids (HBAs) are antioxidant phytochemicals found in rice and effective for the prevention of human diseases including cancer. FBRA, which is a functional food manufactured by fermenting brown rice and rice bran with Aspergillus oryzae, has been demonstrated to have chemopreventive effects against carcinogenesis in various organs. In this study, we developed methods for the relative and absolute quantification of ferulic acid, sinapic acid, caffeic acid, protocatechuic acid and syringic acid in the FBRA and raw material (RM; unfermented brown rice and rice bran) samples by LC/ESI-MS/MS combined with derivatization using a newly developed reagent, N-(2-aminoethyl)-4-(diethylamino)benzamide (ADB) and its deuterium-coded analog, d-ADB...
May 2, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28528575/characterization-of-a-cyclic-olefin-polymer-microcentrifuge-tube
#11
Lloyd Waxman, Rachel L Erwin, Vinod D Vilivalam
Here, we describe the properties of a prototype microcentrifuge tube made from the plastic cyclic olefin polymer (COP). This material has been used in the manufacture of primary containers including syringes and vials for the storage, shipment, and delivery of biotherapeutics, vaccines, and cell therapy products. Its low level of extractable substances and metals along with its glass-like clarity make COP an attractive material for the fabrication of microcentrifuge tubes and other consumable laboratory plasticware where contamination is an important consideration, such as in the storage and analysis of labile proteins, nucleic acids, and metabolites...
May 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28516808/implication-of-foam-sclerosant-inactivation-by-human-whole-blood-in-a-laboratory-setting
#12
Emma B Dabbs, Scott J Dos Santos, Laurensius E Mainsiouw, Alina A Sheikh, Alexis Gkantiragas, Irenie Shiangoli, Mike R Watkins, Jaya L Nemchand, Mark S Whiteley
Background During sclerotherapy, it has been recommended to confirm intravenous placement of the needle by aspirating blood into the sclerosant syringe. This may inactivate some, or all of the sclerosant. Aims To quantify the volume of human blood needed to completely inactivate 1 ml of sodium tetradecyl sulphate, and comparing fresh blood and blood that has been stored in an ethylenediaminetetraacetic acid tube. Methods A series of manual titrations were carried out following a procedure developed at STD Pharmaceutical Products Ltd (Hereford, UK) and listed in the British Pharmacopeia...
January 1, 2017: Phlebology
https://www.readbyqxmd.com/read/28502198/prevalence-of-dentin-hypersensitivity-among-the-residents-of-xi-an-city-china
#13
Xiao Liang, Zhiyuan Wei, Deyu Hu, Jianping Ruan
OBJECTIVE: To investigate the prevalence and intra-oral distribution of dentine hypersensitivity (DH) and to evaluate the related risk factors. MATERIAL AND METHODS: A total of 1320 subjects, aged between 20 and 69 years old, were selected from six communities in the urban areas of Xi'an, China. The data were collected by conducting individual interviews using a standard questionnaire; then, the clinical examination was performed for patients who reported about the discomforts they felt in their teeth when subjected to chemical, mechanical and thermal stimuli...
May 13, 2017: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/28496476/in-vitro-antioxidant-cytotoxic-cholinesterase-inhibitory-activities-and-anti-genotoxic-effects-of-hypericum-retusum-aucher-flowers-fruits-and-seeds-methanol-extracts-in-human-mononuclear-leukocytes
#14
Cumali Keskin, Necmettin Aktepe, Yunus Yükselten, Asuman Sunguroglu, Mehmet Boğa
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28489424/a-novel-ph-responsive-hydrogel-based-on-calcium-alginate-engineered-by-the-previous-formation-of-polyelectrolyte-complexes-pecs-intended-to-vaginal-administration
#15
Natália Noronha Ferreira, Taciane Alvarenga Perez, Liliane Neves Pedreiro, Fabíola Garavello Prezotti, Fernanda Isadora Boni, Valéria Maria de Oliveira Cardoso, Tiago Venâncio, Maria Palmira Daflon Gremião
This work aimed to develop a calcium alginate hydrogel as a pH responsive delivery system for polymyxin B (PMX) sustained-release through the vaginal route. Two samples of sodium alginate from different suppliers were characterized. The molecular weight and M/G ratio determined were, approximately, 107 KDa and 1.93 for alginate_S and 32 KDa and 1.36 for alginate_V. Polymer rheological investigations were further performed through the preparation of hydrogels. Alginate_V was selected for subsequent incorporation of PMX due to the acquisition of pseudoplastic viscous system able to acquiring a differential structure in simulated vaginal microenvironment (pH 4...
May 25, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28484208/an-injectable-non-cross-linked-hyaluronic-acid-gel-containing-therapeutic-spheroids-of-human-adipose-derived-stem-cells
#16
Jingwei Feng, Kazuhide Mineda, Szu-Hsien Wu, Takanobu Mashiko, Kentaro Doi, Shinichiro Kuno, Kahori Kinoshita, Koji Kanayama, Rintaro Asahi, Ataru Sunaga, Kotaro Yoshimura
For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D culture vessel. Spheroids of cells formed after 12 h. We found that 6 × 10(6) ASCs/ml in 3% HA gel achieved the highest spheroid density with appropriate spheroid sizes (20-100 µm)...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28477383/screening-free-radical-scavengers-in-xiexin-tang-by-hplc-abts-dad-q-tof-ms
#17
Yu-Qing Wang, Shu-Jiao Li, Guo Zhuang, Rong-Hui Geng, Xu Jiang
Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown that XXT has therapeutic effects on patients with arteriosclerosis (AS) owing to its antioxidant activity. However, there is little information about the relationship between the chemical composition of XXT and its antioxidant activity. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in XXT...
May 6, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28475362/phytochemical-composition-in-vitro-antioxidant-activity-and-antibacterial-mechanisms-of-neolamarckia-cadamba-fruits-extracts
#18
Arti Pandey, Pradeep Singh Negi
Aqueous extracts of Neolamarckia cadamba fruits prepared at different maturity stages were used for the analysis of various phytochemicals, and their antioxidant and antibacterial activities were determined. Ripe fruit extract had highest phenolics (3.14 mM GAE/ g fruit extract) with caffeic acid, tannic acid, syringic acid and quercetin as major phenolic compounds. The ripe fruit extract showed lowest IC50 values in DPPH radical scavenging assay (231.33 μg fruit extract/ mL), and highest ABTS radical scavenging activity (111...
May 5, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28470323/differential-%C3%AE-amylase-%C3%AE-glucosidase-inhibitory-activities-of-plant-derived-phenolic-compounds-a-virtual-screening-perspective-for-the-treatment-of-obesity-and-diabetes
#19
Hassan Rasouli, Seyed Mohammad-Bagher Hosseini-Ghazvini, Hadi Adibi, Reza Khodarahmi
Recently, due to their biological properties, polyphenol-rich functional foods have been proposed to be unique supplementary and nutraceutical treatments for diabetes mellitus. Inhibition of α-amylase and α-glucosidase enzymes using natural products (especially polyphenols) is a novel oral policy to regulate carbohydrate metabolism and hyperglycemia. The present study aims to evaluate the α-amylase and α-glucosidase inhibitory activity of 26 polyphenols using molecular docking and virtual screening studies...
May 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28468867/medication-errors-by-caregivers-at-home-in-neonates-discharged-from-the-neonatal-intensive-care-unit
#20
Ritanshu Solanki, Nivedita Mondal, Thulasingam Mahalakshmy, Vishnu Bhat
OBJECTIVE: To determine the frequency of medication errors by caregivers at home in neonates discharged from the neonatal intensive care unit and to identify the associated risk factors. METHODS: A descriptive, cross-sectional study was conducted in the High Risk Newborn Follow-up Clinic of our institute, on a sample of 166 children, <3 months old. The medications prescribed (syrup preparations of vitamin D, multivitamins, calcium, iron and levetiracetam, tablet L-thyroxine and ursodeoxycholic acid and human milk fortifier powder) were noted from the discharge summary...
May 3, 2017: Archives of Disease in Childhood
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