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https://www.readbyqxmd.com/read/28231527/rapid-discrimination-and-determination-of-antibiotics-drugs-in-plastic-syringes-using-near-infrared-spectroscopy-with-chemometric-analysis-application-to-amoxicillin-and-penicillin
#1
Laetitia Minh Mai Lê, Luc Eveleigh, Ikram Hasnaoui, Patrice Prognon, Arlette Baillet-Guffroy, Eric Caudron
The aim of this study was to investigate near infrared spectroscopy (NIRS) combined to chemometric analysis to discriminate and quantify three antibiotics by direct measurement in plastic syringes.Solutions of benzylpenicillin (PENI), amoxicillin (AMOX) and amoxicillin/clavulanic acid (AMOX/CLAV) were analyzed at therapeutic concentrations in glass vials and plastic syringes with NIR spectrometer by direct measurement. Chemometric analysis using partial least squares regression and discriminative analysis was conducted to develop qualitative and quantitative calibration models...
February 14, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28231162/phenolic-pro%C3%AF-le-and-antioxidant-activity-of-melon-cucumis-melo-l-seeds-from-pakistan
#2
Alam Zeb
Phenolic composition of different extracts of honeydew melon seeds and their antioxidant activity was determined for the first time. Phenolic compounds were identified using a reversed phase high performance liquid chromatography with diode array detection (HPLC-DAD) method. Results showed the identification of five phenolic compounds in water extract namely gallic acid and its derivative, hydroxybenzoic acid and catechin derivatives and caffeic acid.There were nine phenolic compounds identified in methanol-water extract, which are caffeic acid, two vanillic acid derivatives, ellagitanins, quercetin-3-rutinoside, derivatives of syringic acid and ellagic acid...
October 17, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28216553/an-improved-syringe-agroinfiltration-protocol-to-enhance-transformation-efficiency-by-combinative-use-of-5-azacytidine-ascorbate-acid-and-tween-20
#3
Huimin Zhao, Zilong Tan, Xuejing Wen, Yucheng Wang
Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency. Here, we studied the factors influencing the transformation efficiency of syringe agroinfiltration. The pCAMBIA1301 was transformed into Nicotiana benthamiana leaves for investigation. The effects of 5-azacytidine (AzaC), Ascorbate acid (ASC) and Tween-20 on transformation were studied...
February 14, 2017: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28197166/ectopic-expression-of-the-wild-grape-wrky-transcription-factor-vqwrky52-in-arabidopsis-thaliana-enhances-resistance-to-the-biotrophic-pathogen-powdery-mildew-but-not-to-the-necrotrophic-pathogen-botrytis-cinerea
#4
Xianhang Wang, Rongrong Guo, Mingxing Tu, Dejun Wang, Chunlei Guo, Ran Wan, Zhi Li, Xiping Wang
WRKY transcription factors are known to play important roles in plant responses to biotic stresses. We previously showed that the expression of the WRKY gene, VqWRKY52, from Chinese wild Vitis quinquangularis was strongly induced 24 h post inoculation with powdery mildew. In this study, we analyzed the expression levels of VqWRKY52 following treatment with the defense related hormones salicylic acid (SA) and methyl jasmonate, revealing that VqWRKY52 was strongly induced by SA but not JA. We characterized the VqWRKY52 gene, which encodes a WRKY III gene family member, and found that ectopic expression in Arabidopsis thaliana enhanced resistance to powdery mildew and Pseudomonas syringae pv...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196868/an-interaction-between-the-inner-rod-protein-ysci-and-the-needle-protein-yscf-is-required-to-assemble-the-needle-structure-of-the-yersinia-type-three-secretion-system
#5
Shi-Yang Cao, Wan-Bin Liu, Ya-Fang Tan, Hui-Ying Yang, Ting-Ting Zhang, Tong Wang, Xiao-Yi Wang, Ya-Jun Song, Rui-Fu Yang, Zong-Min Du
The type III secretion system is a highly conserved virulence mechanism that is widely distributed in Gram-negative bacteria. It has a syringe-like structure composed of a multi-ring basal body that spans the bacterial envelope, and a projecting needle that delivers virulence effectors into host cells. Here, we showed that the Yersinia inner rod protein YscI directly interacts with the needle protein YscF inside the bacterial cells, and that this interaction depends on amino acid residues 83-102 in the carboxyl-terminus of YscI...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28191928/self-assembling-multidomain-peptide-nanofibers-for-delivery-of-bioactive-molecules-and-tissue-regeneration
#6
Amanda N Moore, Jeffrey D Hartgerink
Multidomain peptides (MDPs) are a class of self-assembling peptides that are organized in a β-sheet motif, resulting in a nanofibrous architecture. This structure is stabilized by hydrophobic packing in the fiber core and a hydrogen-bonding network down the fiber long axis. Under easily controllable conditions, regulated by electrostatic interactions between the peptides and the pH and salt composition of the solvent, the nanofiber length can be dramatically extended, resulting in fiber entanglement and hydrogel formation...
February 13, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28187689/evaluation-of-antihypertensive-potential-of-ficus-carica-fruit
#7
Alamgeer, Shifa Iman, Hira Asif, Muhammad Saleem
CONTEXT: Ficus carica L. (Moraceae) fruit is said to possess cardiovascular activity and has been used empirically in traditional phytotherapies for the treatment of hypertension and various other cardiovascular diseases. OBJECTIVE: This study investigated the antihypertensive and cardioinhibitory activity of the aqueous-methanol extract of F. carica fruit in rats. MATERIALS AND METHODS: Extract in 250, 500 and 1000 mg/kg doses (p.o.) were administered to normotensive Sprague Dawley rats and blood pressure was measured using non-invasive technique...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28182054/design-formulation-and-physicochemical-evaluation-of-periodontal-propolis-mucoadhesive-gel
#8
Abolfazl Aslani, Negar Malekpour
BACKGROUND: Periodontitis is a disease of tooth supporting tissues, and Gram-negative Bacteria are the main cause of this. Propolis has antibacterial, anti-inflammatory, and antioxidant effects due to its high polyphenol and flavonoids content. The aim of this study is the formulation of a mucoadhesive gel containing concentrated extract of propolis for treatment of periodontitis. MATERIALS AND METHODS: Formulations containing carbopol 940, sodium carboxymethylcellulose (NaCMC), hydroxypropyl methylcellulose K4M, and propolis extract were prepared...
November 2016: Dental Research Journal
https://www.readbyqxmd.com/read/28161229/-secondary-megaureter-a-rare-complication-of-deflux-%C3%A2-endoscopic-management-of-vesicoureteral-reflux-in-children
#9
N Boudaoud, A Line, M Pons, F Lefebvre, M A Bouche Pillon, C Francois, M L Poli Merol
Endoscopic management is the gold standard for symptomatic low-grade vesicoureteral reflux (VUR) in children. Deflux(®) (hyaluronic acid/dextranomer) injection is highly effective and has very few complications. We report on two cases of secondary megaureter after Deflux(®) injections. In the first case, a boy presented with Grade 4 VUR. He received a bilateral Deflux(®) injection with a total of three syringes. The postoperative ultrasound was normal. However, a check-up ultrasound 3 years later showed a significant ureteropyelocalyceal dilatation, with stasis and decreased renal function on scintigraphy, the reason why antireflux surgery (Cohen procedure) was performed...
February 1, 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/28157221/microbial-modulation-of-bacoside-a-biosynthetic-pathway-and-systemic-defense-mechanism-in-bacopa-monnieri-under-meloidogyne-incognita-stress
#10
Rupali Gupta, Akanksha Singh, Madhumita Srivastava, Vivek Singh, M M Gupta, Rakesh Pandey
Plant-associated beneficial microbes have been explored to fulfill the imperative function for plant health. However, their impact on the host secondary metabolite production and nematode disease management remains elusive. Our present work has shown that chitinolytic microbes viz., Chitiniphilus sp. MTN22 and Streptomyces sp. MTN14 singly as well as in combination modulated the biosynthetic pathway of bacoside A and systemic defense mechanism against Meloidogyne incognita in Bacopa monnieri. Interestingly, expression of bacoside biosynthetic pathway genes (3-Hydroxy-3-methylglutaryl coenzyme A reductase, mevalonate diphosphate decarboxylase, and squalene synthase) were upregulated in plants treated with the microbial combination in the presence as well as in absence of M...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28127158/comparison-of-microleakage-and-penetration-depth-between-hydrophilic-and-hydrophobic-sealants-in-primary-second-molar
#11
Pritesh N Gawali, Vishwas B Chaugule, Amey M Panse
INTRODUCTION: Optimal pit and fissure sealing is determined by surface preparation techniques and choice of materials. The performance of pit and fissure sealant materials has been intensively investigated, yet no single product is reported as an ideal sealant. In children, moisture control during cavity preparation is always a big challenge, and hence, hydrophilic sealants have been developed. AIM: To compare the microleakage and penetration depth of hydrophilic and hydrophobic sealants using acid-etching on dry and moist surfaces...
October 2016: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28126679/pacmyba-a-sweet-cherry-r2r3-myb-transcription-factor-is-a-positive-regulator-of-salt-stress-tolerance-and-pathogen-resistance
#12
Xinjie Shen, Xinwei Guo, Xiao Guo, Di Zhao, Wei Zhao, Jingsheng Chen, Tianhong Li
Plant R2R3-MYB transcription factors play crucial roles in stress responses. We previously isolated a R2R3-MYB homolog from sweet cherry cv. Hong Deng, designated PacMYBA (GenBank accession No. KF974774). To explore the role of PacMYBA in the plant stress response, we heterologously expressed PacMYBA in transgenic Arabidopsis thaliana plants. In a previous study, we demonstrated that PacMYBA is mainly localized to the nucleus and could be induced by abscisic acid (ABA). Analysis of the promoter sequence of PacMYBA revealed that it contains several stress-related cis-elements...
January 17, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28121060/combined-application-of-chromatographic-techniques-for-the-separation-of-phenolic-compounds-from-stenoloma-chusanum-ching
#13
Dabing Ren, Binsong Han, Zhongquan Xin, Shasha Ma, Wenbin Liu, Lunzhao Yi
High-speed counter-current chromatography combined with preparative high-performance liquid chromatography was successfully used to separate seven phenolic compounds from Stenoloma chusanum Ching. A biphasic solvent system composed of hexane/ethyl acetate/methanol/water (1:2:1:2, v/v) was used for the first step high-speed counter-current chromatography separation in elution-extrusion mode. A mobile phase composed of acetonitrile (18%) and pure water (82%) was used for further preparative high-performance liquid chromatography purification...
January 25, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28111996/inhibition-behaviours-of-some-phenolic-acids-on-rat-kidney-aldose-reductase-enzyme-an-in-vitro-study
#14
Zuhal Alim, Namik Kilinç, Bülent Şengül, Şükrü Beydemir
Aldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2'5' ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, α-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28107828/dissolvable-layered-double-hydroxide-as-an-efficient-nanosorbent-for-centrifugeless-air-agitated-dispersive-solid-phase-extraction-of-potentially-toxic-metal-ions-from-bio-fluid-samples
#15
Maryam Rajabi, Somayeh Arghavani-Beydokhti, Behruz Barfi, Alireza Asghari
In the present work, a novel nanosorbent namely layered double hydroxides with 4-amino-5-hydroxyl-2,7-naphthalendisulfonic acid monosodium salt interlayer anion (Mg-Al-AHNDA-LDH) was synthesized and applied as a dissolvable nanosorbent in a centrifugeless ultrasound-enhanced air-agitated dispersive solid-phase extraction (USE-AA-D-SPE) method. This method was used for the separation and preconcentration of some metal ions including Cd(2+), Cr(6+), Pb(2+), Co(2+), and Ni(2+) prior to their determination using the micro-sampling flame atomic absorption spectrometry (MS-FAAS) technique...
March 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28101725/sustained-release-delivery-system-of-a-slow-hydrogen-sulfide-donor-gyy-4137-for-potential-application-in-glaucoma
#16
A Patil, S Singh, C Opere, A Dash
Hydrogen sulfide (H2S) targets both underlying factors in glaucoma pathogenesis by reducing elevated intraocular pressure (IOP) and providing retinal neuroprotection, whereas the current clinical approaches targets only reducing IOP. Therefore, H2S could be a potential superior candidate for glaucoma pharmacotherapy. However, H2S could be toxic in a concentration greater than 200 μM and its donors are unstable in water. Therefore, this study investigated the preparation and characterization of a non-aqueous in situ gelling sustained-release delivery system for H2S donors...
January 18, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28032102/evaluation-of-oxalic-acid-treatments-against-the-mite-varroa-destructor-and-secondary-effects-on-honey-bees-apis-mellifera
#17
Noureddine Adjlane, El-Ounass Tarek, Nizar Haddad
BACKGROUND: The Varroa destructor varroasis is a very serious parasite of honeybee Apis mellifera. The objective of this study was to evaluate the effectiveness of Varroa treatment using organic acid (oxalic acid) in Algeria identifying its side effects on bee colonies. METHODS: Treatment was conducted in one apiary consisting 30 colonies kept in Langstroth hives kind. Oxalic acid dripped directly on bees 5ml of this solution of oxalic acid per lane occupied by a syringe...
December 2016: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/28012309/development-of-new-efficient-method-for-isolation-of-phenolics-from-sea-algae-prior-to-their-rapid-resolution-liquid-chromatographic-tandem-mass-spectrometric-determination
#18
Bořivoj Klejdus, Merichel Plaza, Marie Šnóblová, Lea Lojková
The extraction of phenolic compounds from 4 different sea algae samples, three brown algae (Cystoseira abies-marina, C. abies-marina grinded under cryogenic conditions with liquid nitrogen, Undaria pinnatifida and Sargassum muticum) and one red algae (Chondrus crispus) via solid phase extraction using micro-elution solid-phase extraction (μ-SPE) plate method was studied. Prior to μ-SPE, 50mg of algae with 80% methanol mixture was extracted in hyphenated series by various extraction techniques, such as pressurized liquid extraction and Ika Ultra-Turrax(®) Tube Drive, in combination with ultrasound assisted extraction...
December 14, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27979256/synthesis-and-in-vitro-antioxidant-and-antimicrobial-studies-of-novel-structured-phosphatidylcholines-with-phenolic-acids
#19
Marrapu Balakrishna, Shiva Shanker Kaki, Mallampalli S L Karuna, Sripada Sarada, C Ganesh Kumar, R B N Prasad
Novel phenoylated phosphatidylcholines were synthesized from 1,2-dipalmitoyl phosphatidylcholine/egg 1,2-diacyl phosphatidylcholine and phenolic acids such as ferulic, sinapic, vanillic and syringic acids. The structures of phenoylated phosphatidylcholines were confirmed by spectral analysis. 2-acyl-1-lyso phosphatidylcholine was synthesized from phosphatidylcholine via regioselective enzymatic hydrolysis and was reacted with hydroxyl protected phenolic acids to produce corresponding phenoylated phosphatidylcholines in 48-56% yields...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27979114/wheat-bread-enriched-with-green-coffee-in-vitro-bioaccessibility-and-bioavailability-of-phenolics-and-antioxidant-activity
#20
Michał Świeca, Urszula Gawlik-Dziki, Dariusz Dziki, Barbara Baraniak
The potential bioaccessibility and bioavailability of phenolics, caffeine and antioxidant activity of wheat bread enriched with green coffee were studied. Supplementation enhanced nutraceutical potential by improving phenolic content and lipid protecting capacity. The simulated-digestion-released phenolics (mainly caffeic acid, syringic acid and vanillic acid) from bread, also caused significant qualitative changes (chlorogenic acids were cleaved and significant amounts of caffeic acid and ferulic acid were determined)...
April 15, 2017: Food Chemistry
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