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https://www.readbyqxmd.com/read/28516808/implication-of-foam-sclerosant-inactivation-by-human-whole-blood-in-a-laboratory-setting
#1
Emma B Dabbs, Scott J Dos Santos, Laurensius E Mainsiouw, Alina A Sheikh, Alexis Gkantiragas, Irenie Shiangoli, Mike R Watkins, Jaya L Nemchand, Mark S Whiteley
Background During sclerotherapy, it has been recommended to confirm intravenous placement of the needle by aspirating blood into the sclerosant syringe. This may inactivate some, or all of the sclerosant. Aims To quantify the volume of human blood needed to completely inactivate 1 ml of sodium tetradecyl sulphate, and comparing fresh blood and blood that has been stored in an ethylenediaminetetraacetic acid tube. Methods A series of manual titrations were carried out following a procedure developed at STD Pharmaceutical Products Ltd (Hereford, UK) and listed in the British Pharmacopeia...
January 1, 2017: Phlebology
https://www.readbyqxmd.com/read/28502198/prevalence-of-dentin-hypersensitivity-among-the-residents-of-xi-an-city-china
#2
Xiao Liang, Zhiyuan Wei, Deyu Hu, Jianping Ruan
OBJECTIVE: To investigate the prevalence and intra-oral distribution of dentine hypersensitivity (DH) and to evaluate the related risk factors. MATERIAL AND METHODS: A total of 1320 subjects, aged between 20 and 69 years old, were selected from six communities in the urban areas of Xi'an, China. The data were collected by conducting individual interviews using a standard questionnaire; then, the clinical examination was performed for patients who reported about the discomforts they felt in their teeth when subjected to chemical, mechanical and thermal stimuli...
May 13, 2017: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/28496476/in-vitro-antioxidant-cytotoxic-cholinesterase-inhibitory-activities-and-anti-genotoxic-effects-of-hypericum-retusum-aucher-flowers-fruits-and-seeds-methanol-extracts-in-human-mononuclear-leukocytes
#3
Cumali Keskin, Necmettin Aktepe, Yunus Yükselten, Asuman Sunguroglu, Mehmet Boğa
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28489424/a-novel-ph-responsive-hydrogel-based-on-calcium-alginate-engineered-by-the-previous-formation-of-polyelectrolyte-complexes-pecs-intended-to-vaginal-administration
#4
Natália Noronha Ferreira, Taciane Alvarenga Perez, Liliane Neves Pedreiro, Fabíola Garavello Prezotti, Fernanda Isadora Boni, Valéria Maria de Oliveira Cardoso, Tiago Venâncio, Maria Palmira Daflon Gremião
This work aimed to develop a calcium alginate hydrogel as a pH responsive delivery system for polymyxin B (PMX) sustained-release through the vaginal route. Two samples of sodium alginate from different suppliers were characterized. The molecular weight and M/G ratio determined were, approximately, 107 KDa and 1.93 for alginate_S and 32 KDa and 1.36 for alginate_V. Polymer rheological investigations were further performed through the preparation of hydrogels. Alginate_V was selected for subsequent incorporation of PMX due to the acquisition of pseudoplastic viscous system able to acquiring a differential structure in simulated vaginal microenvironment (pH 4...
May 10, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28484208/an-injectable-non-cross-linked-hyaluronic-acid-gel-containing-therapeutic-spheroids-of-human-adipose-derived-stem-cells
#5
Jingwei Feng, Kazuhide Mineda, Szu-Hsien Wu, Takanobu Mashiko, Kentaro Doi, Shinichiro Kuno, Kahori Kinoshita, Koji Kanayama, Rintaro Asahi, Ataru Sunaga, Kotaro Yoshimura
For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D culture vessel. Spheroids of cells formed after 12 h. We found that 6 × 10(6) ASCs/ml in 3% HA gel achieved the highest spheroid density with appropriate spheroid sizes (20-100 µm)...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28477383/screening-free-radical-scavengers-in-xiexin-tang-by-hplc-abts-dad-q-tof-ms
#6
Yu-Qing Wang, Shu-Jiao Li, Guo Zhuang, Rong-Hui Geng, Xu Jiang
Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown that XXT has therapeutic effects on patients with arteriosclerosis (AS) owing to its antioxidant activity. However, there is little information about the relationship between the chemical composition of XXT and its antioxidant activity. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in XXT...
May 6, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28475362/phytochemical-composition-in-vitro-antioxidant-activity-and-antibacterial-mechanisms-of-neolamarckia-cadamba-fruits-extracts
#7
Arti Pandey, Pradeep Singh Negi
Aqueous extracts of Neolamarckia cadamba fruits prepared at different maturity stages were used for the analysis of various phytochemicals, and their antioxidant and antibacterial activities were determined. Ripe fruit extract had highest phenolics (3.14 mM GAE/ g fruit extract) with caffeic acid, tannic acid, syringic acid and quercetin as major phenolic compounds. The ripe fruit extract showed lowest IC50 values in DPPH radical scavenging assay (231.33 μg fruit extract/ mL), and highest ABTS radical scavenging activity (111...
May 5, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28470323/differential-%C3%AE-amylase-%C3%AE-glucosidase-inhibitory-activities-of-plant-derived-phenolic-compounds-a-virtual-screening-perspective-for-the-treatment-of-obesity-and-diabetes
#8
Hassan Rasouli, Seyed Mohammad-Bagher Hosseini-Ghazvini, Hadi Adibi, Reza Khodarahmi
Recently, due to their biological properties, polyphenol-rich functional foods have been proposed to be unique supplementary and nutraceutical treatments for diabetes mellitus. Inhibition of α-amylase and α-glucosidase enzymes using natural products (especially polyphenols) is a novel oral policy to regulate carbohydrate metabolism and hyperglycemia. The present study aims to evaluate the α-amylase and α-glucosidase inhibitory activity of 26 polyphenols using molecular docking and virtual screening studies...
May 4, 2017: Food & Function
https://www.readbyqxmd.com/read/28468867/medication-errors-by-caregivers-at-home-in-neonates-discharged-from-the-neonatal-intensive-care-unit
#9
Ritanshu Solanki, Nivedita Mondal, Thulasingam Mahalakshmy, Vishnu Bhat
OBJECTIVE: To determine the frequency of medication errors by caregivers at home in neonates discharged from the neonatal intensive care unit and to identify the associated risk factors. METHODS: A descriptive, cross-sectional study was conducted in the High Risk Newborn Follow-up Clinic of our institute, on a sample of 166 children, <3 months old. The medications prescribed (syrup preparations of vitamin D, multivitamins, calcium, iron and levetiracetam, tablet L-thyroxine and ursodeoxycholic acid and human milk fortifier powder) were noted from the discharge summary...
May 3, 2017: Archives of Disease in Childhood
https://www.readbyqxmd.com/read/28449398/feeding-induced-phenol-production-in-capsicum-annuum-l-influences-spodoptera-litura-f-larval-growth-and-physiology
#10
Vijaya Movva, Usha Rani Pathipati
We studied the role of induced plant phenols as a defense response to insect herbivory. Phenolic compounds were induced in Capsicum annuum L., the source of many culinary peppers, after feeding by different stages of the insect pest, Spodoptera litura F. The phenols were identified and quantified using high performance liquid chromatography (HPLC) and effects produced by these phenols on larval development were studied. Vanillic acid was identified in plants challenged by second, fourth, and fifth instar larvae, but not in plants challenged by third instar nor unchallenged plants...
May 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28444960/in-vivo-absorption-and-metabolism-of-leptosperin-and-methyl-syringate-abundantly-present-in-manuka-honey
#11
Akari Ishisaka, Shinichi Ikushiro, Mie Takeuchi, Yukako Araki, Maki Juri, Yui Yoshiki, Yoshichika Kawai, Toshio Niwa, Noritoshi Kitamoto, Toshiyuki Sakaki, Hirohito Ishikawa, Yoji Kato
SCOPE: Manuka honey, which shows strong non-peroxide-dependent antibacterial activity, contains unique components, such as methyl syringate 4-O-β-D-gentiobioside (leptosperin) and its aglycone, methyl syringate (MSYR). To determine the potential for biological activity evoked by the ingestion of leptosperin and MSYR, we investigated the absorption and metabolism of these components in manuka honey. METHODS AND RESULTS: The incubation of MSYR with liver microsomes or S9 fractions in vitro resulted in the formation of MSYR-glucuronide (MSYR-GA), MSYR-sulfate (MSYR-S), and syringic acid as metabolites...
April 26, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28416417/in-vitro-evaluation-of-nasogastric-ng-tube-delivery-performance-of-esomeprazole-magnesium-delayed-release-capsules
#12
Alicia Hoover, Dajun Sun, Hong Wen, Wenlei Jiang, Minglei Cui, Xiaojian Jiang, David Keire, Changning Guo
Enteral feeding tubes are used to deliver food or drugs to patients that cannot swallow. To deliver delayed-release drugs that are formulated as enteric coated granules to these patients via feeding tubes requires that they be suspended in water prior to administration. Importantly, the suspension of enteric granules in water of varying pH can cause damage to the enteric coating and affect the bioavailability of the drug. Here, analytical methods for testing acid resistance stability and particle size distribution (PSD) of esomeprazole granules were utilized to monitor the integrity of the granule enteric coating after water pretreatment and delivery through an oral syringe and nasogastric (NG) tube...
April 14, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28401458/purification-characterization-and-thermostability-improvement-of-xylanase-from-bacillus-amyloliquefaciens-and-its-application-in-pre-bleaching-of-kraft-pulp
#13
Sharad Kumar, Izharul Haq, Jyoti Prakash, Sudheer Kumar Singh, Shivaker Mishra, Abhay Raj
Xylanases have important industrial applications but are most extensively utilized in the pulp and paper industry as a pre-bleaching agent. We characterized a xylanase from Bacillus amyloliquefaciens strain SK-3 and studied it for kraft pulp bleaching. The purified enzyme had a molecular weight of ~50 kDa with optimal activity at pH 9.0 and 50 °C. The enzyme showed good activity retention (85%) after 2 h incubation at 50 °C and pH 9.0. This enzyme obeyed Michaelis-Menten kinetics with regard to beechwood xylan with K m and V max values of 5...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28391725/identification-and-in-vitro-antioxidant-activities-of-phenolic-compounds-isolated-from-cynoglossum-cheirifolium-l
#14
Bensaid Ilhem, Atik Bekkara Fawzia, El Haci Imad Abdelhamid, Belarbi Karima, Beddou Fawzia, Bekhechi Chahrazed
In an extensive search for bioactive compounds from plant sources, the quantitative and qualitative characterisation of the compounds present in Cynoglossum cheirifolium extracts was studied. Total phenolic and flavonoid contents were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined through DPPH(•) scavenging activity and Ferric reducing antioxidant power (FRAP). Our study showed that leaves produce more phenolic compounds, followed by flowering aerial part...
April 9, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28391711/phenolic-content-and-antioxidant-properties-of-seeds-from-different-grape-cultivars-grown-in-iran
#15
Vasigheh Sadat Mirbagheri, Ebrahim Alizadeh, Mostafa Yousef Elahi, Sedigheh Esmaeilzadeh Bahabadi
This study investigated the antioxidant activity and phenolic composition of seed extracts from three grape cultivars grown in Iran. Folin Ciocalteu method was used for the determination of the total phenolic contents and GC-MS was used for the analysis of phenolic compositions. 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to evaluate the antioxidant activity. The highest and the lowest total phenolic contents of seed extract were found in the black and green grape, respectively. The content of individual phenols such as Frulic acid, Gentistic acid, Syringic acid, (+) Catechin, Chlorogenic acid and (-)- Epicatchin gallate was cultivars dependent...
April 10, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28390277/enhancement-in-multiple-lignolytic-enzymes-production-for-optimized-lignin-degradation-and-selectivity-in-fungal-pretreatment-of-sweet-sorghum-bagasse
#16
Vartika Mishra, Asim K Jana, Mithu Maiti Jana, Antriksh Gupta
The objective of this work was to study the increase in multiple lignolytic enzyme productions through the use of supplements in combination in pretreatment of sweet sorghum bagasse (SSB) by Coriolus versicolor such that enzymes act synergistically to maximize the lignin degradation and selectivity. Enzyme activities were enhanced by metallic salts and phenolic compound supplements in SSF. Supplement of syringic acid increased the activities of LiP, AAO and laccase; gallic acid increased MnP; CuSO4 increased laccase and PPO to improve the lignin degradations and selectivity individually, higher than control...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28373573/structure-of-aryl-o-demethylase-offers-molecular-insight-into-a-catalytic-tyrosine-dependent-mechanism
#17
Amanda C Kohler, Matthew J L Mills, Paul D Adams, Blake A Simmons, Kenneth L Sale
Some strains of soil and marine bacteria have evolved intricate metabolic pathways for using environmentally derived aromatics as a carbon source. Many of these metabolic pathways go through intermediates such as vanillate, 3-O-methylgallate, and syringate. Demethylation of these compounds is essential for downstream aryl modification, ring opening, and subsequent assimilation of these compounds into the tricarboxylic acid (TCA) cycle, and, correspondingly, there are a variety of associated aryl demethylase systems that vary in complexity...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28361245/detoxification-of-sap-from-felled-oil-palm-trunks-for-the-efficient-production-of-lactic-acid
#18
Balakrishnan Kunasundari, Takamitsu Arai, Kumar Sudesh, Rokiah Hashim, Othman Sulaiman, Natra Joseph Stalin, Akihiko Kosugi
The availability of fermentable sugars in high concentrations in the sap of felled oil palm trunks and the thermophilic nature of the recently isolated Bacillus coagulans strain 191 were exploited for lactic acid production under non-sterile conditions. Screening indicated that strain 191 was active toward most sugars including sucrose, which is a major component of sap. Strain 191 catalyzed a moderate conversion of sap sugars to lactic acid (53%) with a productivity of 1.56 g/L/h. Pretreatment of oil palm sap (OPS) using alkaline precipitation improved the sugar fermentability, providing a lactic acid yield of 92% and productivity of 2...
March 30, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28342478/interactions-between-the-tetrasodium-salts-of-edta-and-1-hydroxyethane-1-1-diphosphonic-acid-with-sodium-hypochlorite-irrigants
#19
Philippe Biel, Dirk Mohn, Thomas Attin, Matthias Zehnder
INTRODUCTION: A clinically useful all-in-one endodontic irrigant with combined proteolytic and decalcifying properties is still elusive. In this study, the chemical effects of dissolving the tetrasodium salts of 1-hydroxyethane 1,1-diphosphonic acid (Na4HEDP) or Na4EDTA directly in sodium hypochlorite (NaOCl) irrigants in polypropylene syringes were assessed during the course of 1 hour. METHODS: The solubility of the salts in water was determined. Their compatibility with 1% and 5% NaOCl was measured by iodometric titration and in a calcium complexation experiment by using a Ca(2+)-selective electrode...
April 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28338616/a-comparative-study-of-phenols-in-apulian-italian-wines
#20
Andrea Ragusa, Carla Centonze, Maria E Grasso, Maria F Latronico, Pier F Mastrangelo, Federica Sparascio, Francesco P Fanizzi, Michele Maffia
Nutraceutics is a growing research field in which researchers study and attempt to improve the biological properties of metabolites in food. Wine is one of the most consumed products in the world and contains a plethora of molecules biologically relevant to human health. In this article, several polyphenols with potential antioxidant activity were measured in wines from Apulia, in Southeast Italy. Hydroxytyrosol, gallic and syringic acids, luteolin, quercetin, and trans-resveratrol were identified and quantified by HPLC...
March 24, 2017: Foods (Basel, Switzerland)
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