keyword
MENU ▼
Read by QxMD icon Read
search

8-hydroxy-2-deoxyguanosine

keyword
https://www.readbyqxmd.com/read/28431387/cypermethrin-toxication-leads-to-histopathological-lesions-and-induces-inflammation-and-apoptosis-in-common-carp-cyprinus-carpio-l
#1
Harun Arslan, Selçuk Özdemir, Serdar Altun
Cypermethrin (Cyp), a known neurotoxic pesticide, is widely used in agricultural applications. In the present study, the aim was to determine the histopathological effects of Cyp toxication and evaluate the activation of inducible nitric oxide synthetase (iNOS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) using an immunofluorescence assay. Thereafter, we identified the expressions of caspase 3, capsase 8, iNOS, and metallothionein 1 (MT1) genes in common carp using quantitative reverse transcription polymerase chain reaction (qRT-PCR)...
April 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28408945/recuperating-lung-decoction-attenuates-the-oxidative-stress-state-of-chronic-obstructive-pulmonary-disease-by-inhibiting-the-mapk-ap-1-signal-pathway-and-regulating-%C3%AE-gcs
#2
Chunlei Li, Qi Shi, Yue Yan, Yanhua Kong, YanYan Meng, Tiezhu Wang, Xing Zhang, Haipeng Bao, Youlin Li
Purpose/Objective. To evaluate the effects of Recuperating Lung Decoction (RLD) on the indices of oxidative stress in a rat model of COPD and detect the indices of the MAPK/AP-1/γ-GCS signal pathway for a further survey of the possible targeting site of RLD. Methods/Materials. The rats of COPD were treated with RLD. The protein levels of glutathione (GSH), oxidized glutathione (GSSG), 8-hydroxy-2-deoxyguanosine (8-OHdG), and 4-hydroxynonenal (4-HNE) were measured. In addition, the levels of key signaling molecules (extracellular signal-regulated kinases [ERK], the c-jun N-terminal kinase [JNKs signal pathway], and p38 MAP kinase [p38MAPK], AP-1 proteins [C-fos, C-jun], and γ-glutamyl-cysteine synthetase [γ-GCS-h]) of the MAPK/AP-1/γ-GCS-h signal pathway were assessed...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28400213/investigation-of-8-ohdg-cyp1a-hsp70-and-transcriptional-analyses-of-antioxidant-defence-system-in-liver-tissues-of-rainbow-trout-exposed-to-eprinomectin
#3
Gonca Alak, Aslı Çilingir Yeltekin, Ismail Hakkı Taş, Arzu Ucar, Veysel Parlak, Ahmet Topal, Esat Mahmut Kocaman, Muhammed Atamanalp
Eprinomectin (EPM), a member of avermectin family, is a semi-synthetic antibiotic. It has been known that avermectin family enters the aquatic environments and adversely affects the aquatic organisms. Effects of EPM is fully unknown in aquatic organisms especially fish, thus the aim of the present study was to investigate transcriptional changes (sod, cat, gpx) and activities of some antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)) and transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A) in liver tissues of rainbow trout exposed to sublethal EPM concentration (0...
April 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28399480/acute-toxication-of-deltamethrin-results-in-activation-of-inos-8-ohdg-and-up-regulation-of-caspase-3-inos-gene-expression-in-common-carp-cyprinus-carpio-l
#4
Harun Arslan, Serdar Altun, Selçuk Özdemir
Deltamethrin is a widely used synthetic pyrethroid pesticide that protects agricultural yields, including crops, fruits, and vegetables from insect-pests. It is known that deltamethrin toxication leads to metabolic disorders and has detrimental effects on the brain and liver in different organisms. However, the harmful effects of deltamethrin toxication on aquatic animals remain unclear. In the present study, we aimed to evaluate the adverse effects of deltamethrin toxication by performing a histopathological examination, an immunofluorescence assay, and a qRT-PCR on common carp...
April 2, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28345375/the-chemo-prophylactic-efficacy-of-an-ethanol-moringa-oleifera-leaf-extract-against-hepatocellular-carcinoma-in-rats
#5
Kadry M Sadek, Tarek K Abouzed, Reham Abouelkhair, Sherif Nasr
CONTEXT: Hepatocellular carcinoma (HCC) is among the most well-known threatening tumours around the world, and the outlook remains bleak. Moringa oleifera Lam. (Moringaceae) exhibits antitumor, antioxidant and hepatoprotective properties. OBJECTIVES: To assess the chemo-prophylactic proficiency and other likely activities of Moringa oleifera leaf ethanol extract (MOLEE) against diethyl nitrosamine (DEN)-induced HCC. MATERIALS AND METHODS: Wistar rats were gastrogavaged with MOLEE (500 mg/kg) for one week and then gastrogavaged with MOLEE and DEN (10 mg/kg) for the following 16 weeks...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28341989/a-fully-validated-bioanalytical-method-using-an-uhplc-ms-ms-system-for-quantification-of-dna-and-rna-oxidative-stress-biomarkers
#6
Barbora Cervinkova, Lenka Kujovska Krcmova, Veronika Sestakova, Dagmar Solichova, Petr Solich
A new, rapid and effective ultra-high-performance liquid chromatography method with mass spectrometry detection is described for the separation and quantification of 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine and creatinine in human urine. The present study uses an isotope-labelled internal standard ([(15)N]5-8-hydroxy-2-deoxyguanosine), a BIO core-shell stationary phase and an isocratic elution of methanol and water. Sample preparation of human urine was performed by solid-phase extraction (SPE) on Oasis HLB cartridges with methanol/water 50:50 (v/v) elution...
March 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28277069/hyperglycaemia-oxidative-stress-and-inflammatory-markers
#7
Eugene G Butkowski, Herbert F Jelinek
INTRODUCTION: The increasing prevalence of hyperglycaemia implicates a state of oxidative stress and inflammation. Traditional and emerging biomarkers associated with increasing hyperglycaemia were assessed to clarify their role they play in hyperglycaemia. RESULTS: 309 participants attending a rural diabetic screening program were categorised into control and quintile groups based upon glucose levels: 1st quintile - <4.5 mmol/L and 4th, 5th quintile - >6...
September 22, 2016: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28263525/markers-of-nucleic-acids-and-proteins-oxidation-among-office-workers-exposed-to-air-pollutants-including-nano-tio2-particles
#8
Daniela Pelclova, Vladimir Zdimal, Petr Kacer, Stepanka Vlckova, Zdenka Fenclova, Tomas Navratil, Martin Komarc, Jaroslav Schwarz, Nadezda Zikova, Otakar Makes, Sergey Zakharov
OBJECTIVES: Experimental studies using nanoscale TiO2 have documented lung injury, inflammation, oxidative stress, and genotoxicity. Human health data are extremely scarce. METHODS: In exhaled breath condensate (EBC) and urine of 22 office employees occupationally exposed to TiO2 during their visit in the production workshops for average 14±9 min/day a panel of biomarkers of nucleic acids and proteins oxidation was studied, specifically 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr)...
December 18, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28235412/the-herbal-formula-kh-204-is-protective-against-erectile-dysfunction-by-minimizing-oxidative-stress-and-improving-lipid-profiles-in-a-rat-model-of-erectile-dysfunction-induced-by-hypercholesterolaemia
#9
Hoon Jang, Woong Jin Bae, Su Jin Kim, Hyuk Jin Cho, Seung Mo Yuk, Dong Seok Han, Chang Shik Youn, Eun Bi Kwon, Sung Yeoun Hwang, Sae Woong Kim
BACKGROUND: Hypercholesterolaemia (HC) is a major risk factor for ischemic heart disease and is also known to be a risk factor for erectile dysfunction (ED). ED caused by HC is thought to be related to HC-induced oxidative stress damage in the vascular endothelium and erectile tissue. KH-204 is an herbal formula with a strong antioxidant effect. We evaluated the effects of KH-204 on erectile function in a rat model of HC-induced ED. METHODS: Male Sprague-Dawley rats (6 weeks old) were divided into normal control, high-fat and cholesterol diet (HFC), and HFC with KH-204 treatment (HFC + KH) groups (n = 12 each)...
February 24, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28219821/neurotoxic-responses-in-brain-tissues-of-rainbow-trout-exposed-to-imidacloprid-pesticide-assessment-of-8-hydroxy-2-deoxyguanosine-activity-oxidative-stress-and-acetylcholinesterase-activity
#10
Ahmet Topal, Gonca Alak, Mustafa Ozkaraca, Aslı Cilingir Yeltekin, Selim Comaklı, Gurdal Acıl, Mine Kokturk, Muhammed Atamanalp
The extensive use of imidacloprid, a neonicotinoid insecticide, causes undesirable toxicity in non-targeted organisms including fish in aquatic environments. We investigated neurotoxic responses by observing 8-hydroxy-2-deoxyguanosine (8-OHdG) activity, oxidative stress and acetylcholinesterase (AChE) activity in rainbow trout brain tissue after 21 days of imidacloprid exposure at levels of (5 mg/L, 10 mg/L, 20 mg/L). The obtained results indicated that 8-OHdG activity did not change in fish exposed to 5 mg/L of imidacloprid, but 10 mg/L and 20 mg/L of imidacloprid significantly increased 8-OHdG activity compared to the control (p < 0...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28110317/the-effect-of-sunblock-against-oxidative-stress-in-farmers-a-pilot-study
#11
Kim Yong-Dae, Yim Dong-Hyuk, Eom Sang-Yong, Yeoun Lee Ji, Kim Heon
Farmers are frequently exposed to ultraviolet (UV) radiation which causes various diseases by inducing oxidative stress. This study aimed to assess the effects of sunblock on oxidative stress in the body. Eighty-seven farmers were divided into two groups: those who wore sunblock for five days and those who did not. The total antioxidant capacity (TAC) in urine, which is an antioxidant indicator, and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels in urine, an oxidative stress indicator, were measured. The urinary TAC of sunblock users was significantly higher than that of non-users, but urinary 8-OHdG levels were not significantly different...
January 19, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28065503/8-hydroxy-2-deoxyguanosine-levels-and-heart-failure-a-systematic-review-and-meta-analysis-of-the-literature
#12
A Di Minno, L Turnu, B Porro, I Squellerio, V Cavalca, E Tremoli, M N D Di Minno
BACKGROUND AND AIMS: The generation of reactive oxygen species (ROS) plays an important role in the etiology of several pathological conditions. High levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), a biomarker of oxidative damage of DNA, have been found in patients with heart failure (HF). We performed a meta-analysis of the literature to investigate the association between 8-OHdG levels and HF. METHODS AND RESULTS: A systematic search was performed in the PubMed, Web of Science, Scopus, EMBASE databases and studies evaluating 8-OHdG levels in HF patients and controls were included...
November 20, 2016: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28062958/a-study-of-oxidative-stress-in-neonates-delivered-through-meconium-stained-amniotic-fluid
#13
Tapas Bandyopadhyay, B D Bhatia, H D Khanna
To estimate the levels of malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OH-dG) in cord blood plasma of newborns born through meconium-stained amniotic fluid (MSAF) and also to find out the correlation between their levels with birth weight and gestation, we measured the cord blood plasma levels of MDA and 8-OH-dG in 59 newborns born through MSAF and 50 newborns born through clear liquor. The levels of cord blood plasma MDA and 8-OH-dG were significantly higher in full-term and late-preterm newborns born through MSAF...
January 6, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28007572/methane-ameliorates-spinal-cord-ischemia-reperfusion-injury-in-rats-antioxidant-anti-inflammatory-and-anti-apoptotic-activity-mediated-by-nrf2-activation
#14
Liping Wang, Ying Yao, Rong He, Yan Meng, Na Li, Dan Zhang, Jiajun Xu, Ouyang Chen, Jin Cui, Jinjun Bian, Yan Zhang, Guozhong Chen, Xiaoming Deng
Methane is reported to have antioxidant, anti-inflammatory and anti-apoptotic properties. We investigated the potential neuroprotective effects of methane-rich saline (MS) on spinal cord ischemia-reperfusion injury and determined that its therapeutic benefits are associated with the activation of nuclear factor erythroid 2-related factor 2 (Nrf2). Rats received 9min of spinal cord ischemia induced by occlusion of the descending thoracic aorta plus systemic hypotension followed by a single MS treatment (10ml/kg, ip) and 72h reperfusion...
December 20, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27993476/urinary-oxidative-stress-biomarkers-among-local-residents-measured-6years-after-the-hebei-spirit-oil-spill
#15
Jung-Ah Kim, Su Ryeon Noh, Hae-Kwan Cheong, Mina Ha, Sang-Yong Eom, Heon Kim, Myung-Sook Park, Yeonhee Chu, Seung-Hwa Lee, Kyungho Choi
In December 2007, the Hebei Spirit oil spill occurred on the west coast of South Korea. Association between crude oil exposure and the levels of urinary oxidative stress biomarkers was reported among the local residents, 1.5years after the accident. The aim of this study was to follow-up possible longer-term effects of the oil spill among the residents near the oil spill. Six years after the accident, urinary oxidative stress biomarkers of the residents were associated with surrogates of oil exposure. The target subjects were divided into two groups based on the distance of their residence to the spill site, i...
February 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/27909452/the-levels-of-cortisol-and-oxidative-stress-and-dna-damage-in-child-and-adolescent-victims-of-sexual-abuse-with-or-without-post-traumatic-stress-disorder
#16
Şeref Şimşek, Tuğba Yüksel, İbrahim Kaplan, Cem Uysal, Hüseyin Aktaş
OBJECTIVE: The aim of this study was to investigate whether cortisol and oxidative stress levels and DNA damage differ between individuals who developed PTSD or not following a sexual trauma. METHODS: The study included 61 children aged between 5 and 17 years who sustained sexual abuse (M/F: 18/43). The patients were divided into two groups: patients with PTSD and patients without PTSD based, based on the results of a structured psychiatric interview (K-SADS-PL and CAPS-CA)...
November 2016: Psychiatry Investigation
https://www.readbyqxmd.com/read/27906110/failed-upregulation-of-tfam-protein-and-mitochondrial-dna-in-oxidatively-deficient-fibers-of-chronic-obstructive-pulmonary-disease-locomotor-muscle
#17
Yana Konokhova, Sally Spendiff, R Thomas Jagoe, Sudhakar Aare, Sophia Kapchinsky, Norah J MacMillan, Paul Rozakis, Martin Picard, Mylène Aubertin-Leheudre, Charlotte H Pion, Jean Bourbeau, Russell T Hepple, Tanja Taivassalo
BACKGROUND: Low mitochondrial content and oxidative capacity are well-established features of locomotor muscle dysfunction, a prevalent and debilitating systemic occurrence in patients with chronic obstructive pulmonary disease (COPD). Although the exact cause is not firmly established, physical inactivity and oxidative stress are among the proposed underlying mechanisms. Here, we assess the impact of COPD pathophysiology on mitochondrial DNA (mtDNA) integrity, biogenesis, and cellular oxidative capacity in locomotor muscle of COPD patients and healthy controls...
February 18, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27896921/effect-of-iron-supplementation-during-lactation-on-maternal-iron-status-and-oxidative-stress-a-randomized-controlled-trial
#18
Josh M Jorgensen, Zhenyu Yang, Bo Lönnerdal, Caroline J Chantry, Kathryn G Dewey
We examined the effect of iron-containing prenatal vitamin-mineral supplements taken postpartum on biomarkers of iron status and oxidative stress. Lactating women (n = 114) were randomly assigned to consume daily one iron-free prenatal vitamin-mineral supplement plus either 27 mg of iron or placebo for approximately 3.5 months. The placebo group took the tablets between meals, while those given iron took the tablets either with (Fe-W) or between meals (Fe-B). Blood and urine samples were collected before and after the supplementation period to analyze hemoglobin (Hb), ferritin, hepcidin, transferrin saturation (TfSat), total plasma iron, and biomarkers of oxidative stress (isoprostane and 8-hydroxy-2-deoxyguanosine (8-OHdG)) and inflammation (C-reactive protein (CRP) and alpha-1-acid glycoprotein (AGP))...
November 29, 2016: Maternal & Child Nutrition
https://www.readbyqxmd.com/read/27896629/remote-limb-ischemic-preconditioning-protects-rats-against-cerebral-ischemia-via-hif-1%C3%AE-ampk-hsp70-pathway
#19
Ming Xia, Qian Ding, Zhidan Zhang, Qinggen Feng
Remote limb ischemic preconditioning (RIPC) is a clinically feasible strategy to protect against ischemia/reperfusion injury, but the knowledge concerning the mechanism underlying RIPC is scarce. This study was performed to examine the effect of RIPC on brain tissue suffering from ischemia challenge and explore its underlying mechanism in a rat model. The animals were divided into four groups: Sham, middle cerebral artery occlusion (MCAO), RIPC, and MCAO+RIPC. We found that previous exposure to RIPC significantly attenuated neurological dysfunction and lessened brain edema in MCAO+RIPC group...
November 28, 2016: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/27886567/evaluation-of-8-hydroxy-2-deoxyguanosine-and-nfkb-activation-oxidative-stress-response-acetylcholinesterase-activity-and-histopathological-changes-in-rainbow-trout-brain-exposed-to-linuron
#20
Ahmet Topal, Gonca Alak, Serdar Altun, Hüseyin Serkan Erol, Muhammed Atamanalp
Linuron is a widely used herbicide to control grasses and annual broad leaf weeds. It is known that linuron has toxic effects on different organisms. However, the toxic effects of linuron on aquatic organisms, especially fish, is completely unknown. Thus, we aimed to investigate changes in 8-hydroxy-2-deoxyguanosine (8-OHdG) and nuclear factor kappa B (NFkB) activity, histopathological changes, antioxidant responses and acetylcholinesterase (AChE) activity in rainbow trout brain after exposure to linuron. Fish were exposed to 30μg/L, 120μg/L and 240μg/L concentrations of linuron for twenty-one days...
January 2017: Environmental Toxicology and Pharmacology
keyword
keyword
63547
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"