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https://www.readbyqxmd.com/read/26781683/training-response-to-high-intensity-interval-training-in-a-42-year-old-man-with-chronic-spinal-cord-injury
#1
Christopher R Harnish, Jonathan A Daniels, Deborah Caruso
Objective To review the outcome of 12 weeks of periodized, high-intensity interval training (HIT) in a man with chronic traumatic spinal cord injury (SCI). Methods A 42-year-old man (180 cm tall, 68.4 kg and 32.0% Fat) with a C8/T1 motor complete SCI took part in 12 weeks of 3 days per week arm crank ergometry (ACE) interval training. Training consisted of a combination of HIT that included three times 5  min at ∼70% Peak Power (WPeak) and 5  min recovery (HIT5); four times 2.5  min at ∼85% WPeak and 5  min recovery (HIT2...
January 18, 2016: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/25730202/fast-forward-genetics-to-identify-mutations-causing-a-high-light-tolerant-phenotype-in-chlamydomonas-reinhardtii-by-whole-genome-sequencing
#2
Lisa Schierenbeck, David Ries, Kristin Rogge, Sabrina Grewe, Bernd Weisshaar, Olaf Kruse
BACKGROUND: High light tolerance of microalgae is a desired phenotype for efficient cultivation in large scale production systems under fluctuating outdoor conditions. Outdoor cultivation requires the use of either wild-type or non-GMO derived mutant strains due to safety concerns. The identification and molecular characterization of such mutants derived from untagged forward genetics approaches was limited previously by the tedious and time-consuming methods involving techniques such as classical meiotic mapping...
2015: BMC Genomics
https://www.readbyqxmd.com/read/24097174/the-impact-of-high-intensity-intermittent-exercise-on-resting-metabolic-rate-in-healthy-males
#3
Benjamin Kelly, James A King, Jonas Goerlach, Myra A Nimmo
INTRODUCTION: High-intensity intermittent exercise training (HIT) may favourably alter body composition despite low training volumes and predicted energy expenditure (EE). PURPOSE: To characterise the acute impact of two common HIT protocols on EE and post-exercise oxygen consumption (11 h EPOC). METHODS: Oxygen consumption (l min(-1)), respiratory exchange ratio (RER) and EE were measured in nine healthy, lean males over 12 h under three conditions: control (CON), HIT1 (10 × 1 min high-intensity cycling bouts followed by 1 min rest) and HIT2 (10 × 4 min high-intensity cycling bouts followed by 2 min rest)...
December 2013: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/21648101/effects-of-imac-specific-peptide-tags-on-the-stability-of-recombinant-green-fluorescent-protein
#4
Gabriel Widakowich, Chunfang Zhang, Simon Harris, Khosse Mitri, Glenn Powers, Kirk-Ski Troung, Milton T W Hearn
Immobilized metal ion affinity chromatography (IMAC) using peptide affinity tags has become a popular tool for protein purification. An important feature dictating the use of a specific affinity tag is whether its structure influences the properties of the target protein to which it is attached. In this work we have studied the influence on protein stability of two novel peptide affinity tags, namely NT1A and HIT2, and compared their effect to the commonly used hexa-histidine tag, all attached to the C-terminus of a enhanced green fluorescent protein (eGFP)...
July 2011: Biotechnology Progress
https://www.readbyqxmd.com/read/20345641/isolation-and-characterization-of-the-arabidopsis-heat-intolerant-2-hit2-mutant-reveal-the-essential-role-of-the-nuclear-export-receptor-exportin1a-xpo1a-in-plant-heat-tolerance
#5
Shin-Jye Wu, Lian-Chin Wang, Ching-Hui Yeh, Chun-An Lu, Shaw-Jye Wu
*The Arabidopsis heat-intolerant 2 (hit2) mutant was isolated on the basis of its impaired ability to withstand moderate heat stress (37 degrees C). Determination of the genetic mutation that underlies the hit2 thermosensitive phenotype allowed better understanding of the mechanisms by which plants cope with heat stress. *Genetic analysis revealed that hit2 is a single recessive mutation. Map-based cloning was used to identify the hit2 locus. The response of hit2 to other types of heat stress was also investigated to characterize the protective role of HIT2...
June 2010: New Phytologist
https://www.readbyqxmd.com/read/9809494/changes-in-lung-volumes-and-poor-perception-of-bronchoconstriction-induced-respiratory-symptoms
#6
P Catellier, H Turcotte, F Deschesnes, L P Boulet
BACKGROUND: Changes in lung volumes may contribute to the perception of bronchoconstriction-induced breathlessness. AIM: To verify whether histamine-induced increases in lung volumes are more marked in asthmatic subjects with normal compared with low symptom perception scores and whether changes in lung volumes are detected similarly in both groups of subjects. METHODS: A group of 16 asthmatic subjects with low (< or =1) breathlessness score (scale: 0 to 10) at 20% fall in FEV1 (PS20B) on at least one of 2 histamine inhalation tests (HIT) performed on separate days (hypoperceivers) were studied...
October 1998: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/9563655/serum-proteins-with-nad-glycohydrolase-activity-and-anti-cd38-reactivity-elevated-levels-in-serum-of-tumour-patients
#7
C Korkut, L Yalçintepe, N Kiremit-Korkut, S Uzun-Altinöz, S Işsever, F Gümüşel, D Tiryaki, E Bermek
NAD+ glycohydrolase activities in serum samples from cancer patients were two- to three-fold higher than the activities in samples from healthy controls. SDS-PAGE analysis of serum samples followed by Western blotting revealed the presence of two proteins of approximately 45 and approximately 21 kDa that were immunoreactive with human CD38-specific monoclonal antibodies T16, HIT2 and OKT 10. These proteins appeared to be more abundant in serum from cancer patients. NAD+ glycohydrolase activity in serum could be enriched by immunoaffinity chromatography by using T16-Sepharose 4B...
April 10, 1998: Cancer Letters
https://www.readbyqxmd.com/read/2293225/preliminary-observations-on-the-phenotypes-of-lymphoid-cells-in-mycosis-fungoides-using-anti-human-thymocyte-monoclonal-antibodies
#8
J Liu, Y Shi, Q Kong, Q Zhang, J Huang, X Zeng, X Wang, J Li, H Sang, A Li
The phenotypes of lymphoid cells from twelve patients with mycosis fungoides (including 5 patients in the tumor stage and 7 in the pretumor stage) were determined immunohistochemically using anti-thymocyte monoclonal antibodies (HIT1, HIT2, and Leu 6). The results showed significant differences between the numbers of HIT1 and HIT2 positive cells in tumor and pretumor stage lesions, indicating that these two monoclonal antibodies may be of help in the staging of mycosis fungoides and in making a prognosis. Also, we found that some Leu 6 (+) cells in tumor stage lesions were round or oval in shape without dendritic processes, suggesting that they may be immature lymphocytes...
1990: Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College
https://www.readbyqxmd.com/read/1325386/ty-element-induced-temperature-sensitive-mutations-of-saccharomyces-cerevisiae
#9
K Kawakami, B K Shafer, D J Garfinkel, J N Strathern, Y Nakamura
Temperature-sensitive mutants of Saccharomyces cerevisiae were isolated by insertional mutagenesis using the HIS3 marked retrotransposon TyH3HIS3. In such mutants, the TyHIS3 insertions are expected to identify loci which encode genes essential for cell growth at high temperatures but dispensable at low temperatures. Five mutations were isolated and named hit for high temperature growth. The hit1-1 mutation was located on chromosome X and conferred the pet phenotype. Two hit2 mutations, hit2-1 and hit2-2, were located on chromosome III and caused the deletion of the PET18 locus which has been shown to encode a gene required for growth at high temperatures...
August 1992: Genetics
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