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Phagocytic activity of neutrophils

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https://www.readbyqxmd.com/read/28320834/early-intracellular-trafficking-of-granulibacter-bethesdensis-in-human-macrophages
#1
Jessica Chu, Margery G Smelkinson, David W Dorward, Kol A Zarember, John I Gallin
Granulibacter bethesdensis is a Gram-negative bacterium that infects patients with Chronic Granulomatous Disease (CGD), a primary immunodeficiency marked by a defect in NOX2, the phagocyte nicotinamide adenine dinucleotide phosphate oxidase. Previous studies have shown that NOX2 is essential for killing of G. bethesdensis by neutrophils and monocytes and that bacteriostatic activity of monocyte derived macrophages (MDM) requires NOX2 and IFNγ-pretreatment. To determine if G. bethesdensis evades phagolysosomal killing, a host defense pathway intact in both normal and CGD MDM, or occupies a distinct intracellular niche in CGD MDM, we assessed the trafficking patterns of this organism...
March 20, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28293191/an-exploration-of-charge-compensating-ion-channels-across-the-phagocytic-vacuole-of-neutrophils
#2
Juliet R Foote, Philippe Behe, Mathew Frampton, Adam P Levine, Anthony W Segal
Neutrophils phagocytosing bacteria and fungi exhibit a burst of non-mitochondrial respiration that is required to kill and digest the engulfed microbes. This respiration is accomplished by the movement of electrons across the wall of the phagocytic vacuole by the neutrophil NADPH oxidase, NOX2. In this study, we have attempted to identify the non-proton ion channels or transporters involved in charge compensation by examining the effect of inhibitors on vacuolar pH and cross-sectional area, and on oxygen consumption...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28289416/specific-and-efficient-uptake-of-surfactant-free-poly-lactic-acid-nanovaccine-vehicles-by-mucosal-dendritic-cells-in-adult-zebrafish-after-bath-immersion
#3
Julien Rességuier, Emilie Delaune, Anne-Line Coolen, Jean-Pierre Levraud, Pierre Boudinot, Dominique Le Guellec, Bernard Verrier
Activation of mucosal immunity is a key milestone for next-generation vaccine development. Biocompatible polymer-based nanoparticles (NPs) are promising vectors and adjuvants for mucosal vaccination. However, their in vivo uptake by mucosae and their biodistribution in antigen-presenting cells (APCs) need to be better understood to optimize mucosal nanovaccine designs. Here, we assessed if APCs are efficiently targeted in a spontaneous manner by surfactant-free poly(lactic acid) nanoparticles (PLA-NPs) after mucosal administration...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28282603/nicotine-can-modulate-the-effects-of-the-mesenchymal-stem-cells-on-neutrophils
#4
Samira Pourtayeb, Seyyed Meysam Abtahi Froushani
PURPOSE: It has been revealed that mesenchymal stem cells (MSCs) express some of the nicotinic receptor subunits. Moreover, the crosstalk between MSCs and neutrophils is not far-fetched. Therefore, the aim of the present study is to determine the role of nicotine on the effects of MSCs on neutrophils. METHODS: After the isolation of mesenchymal stem cells from the bone marrow of rats, these cells were pulsed with different concentrations of nicotine (0, 0.1, 0.5, and 1μM) for different periods (24, 48, and 72h)...
March 7, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28273167/staphylococcal-protein-ecb-impairs-complement-receptor-1-mediated-recognition-of-opsonized-bacteria
#5
Hanne Amdahl, Karita Haapasalo, Lydia Tan, Taru Meri, Pentti I Kuusela, Jos A van Strijp, Suzan Rooijakkers, T Sakari Jokiranta
Staphyloccus aureus is a major human pathogen leading frequently to sepsis and soft tissue infections with abscesses. Multiple virulence factors including several immune modulating molecules contribute to its survival in the host. When S. aureus invades the human body, one of the first line defenses is the complement system, which opsonizes the bacteria with C3b and attract neutrophils by release of chemotactic peptides. Neutrophils express Complement receptor-1 [CR1, CD35) that interacts with the C3b-opsonized particles and thereby plays an important role in pathogen recognition by phagocytic cells...
2017: PloS One
https://www.readbyqxmd.com/read/28258703/the-cd47-sirp%C3%AE-signaling-axis-as-an-innate-immune-checkpoint-in-cancer
#6
REVIEW
Hanke L Matlung, Katka Szilagyi, Neil A Barclay, Timo K van den Berg
Immune checkpoint inhibitors, including those targeting CTLA-4/B7 and the PD-1/PD-L1 inhibitory pathways, are now available for clinical use in cancer patients, with other interesting checkpoint inhibitors being currently in development. Most of these have the purpose to promote adaptive T cell-mediated immunity against cancer. Here, we review another checkpoint acting to potentiate the activity of innate immune cells towards cancer. This innate immune checkpoint is composed of what has become known as the 'don't-eat me' signal CD47, which is a protein broadly expressed on normal cells and often overexpressed on cancer cells, and its counter-receptor, the myeloid inhibitory immunoreceptor SIRPα...
March 2017: Immunological Reviews
https://www.readbyqxmd.com/read/28258152/chemical-composition-and-phagocyte-immunomodulatory-activity-of-ferula-iliensis-essential-oils
#7
Gulmira Özek, Igor A Schepetkin, Gulzhakhan A Utegenova, Liliya N Kirpotina, Spencer R Andrei, Temel Özek, Kemal Hüsnü Can Başer, Karime T Abidkulova, Svetlana V Kushnarenko, Andrei I Khlebnikov, Derek S Damron, Mark T Quinn
Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)-propenyl sec-butyl disulfide (15.7-39.4%) and (Z)-propenyl sec-butyl disulfide (23.4-45.0%)...
March 3, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28237625/selective-sensitization-of-human-neutrophils-to-lukgh-mediated-cytotoxicity-by-staphylococcus-aureus-and-il-8
#8
Philipp Janesch, Harald Rouha, Susanne Weber, Stefan Malafa, Karin Gross, Barbara Maierhofer, Adriana Badarau, Zehra C Visram, Lukas Stulik, Eszter Nagy
OBJECTIVES: Staphylococcus aureus produces up to five bi-component leukocidins - LukSF-PV, gamma-hemolysins AB and CB, LukGH (LukAB) and LukED - to evade innate immunity by lysing phagocytic cells. Species specificity of these leukocidins limits the relevance of animal models, therefore we assessed their individual contribution using human neutrophils. METHODS: Human polymorphonuclear leukocytes (PMNs) were activated with stimuli relevant during bacterial infections and sensitivity to recombinant leukocidins was measured in cell-viability assays...
February 22, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28230708/cell-derived-nanoparticles-are-endogenous-modulators-of-sepsis-with-therapeutic-potential
#9
Natalia Kunz, Brent T Xia, Kai-Uwe Kalies, Matthias Klinger, Timo Gemoll, Jens K Habermann, Brynne E Whitacre, Aaron P Seitz, Kathrin Kalies, Charles C Caldwell
Cell-derived nanoparticles (CDNPs) containing cytosolic proteins and RNAs/DNAs can be isolated from stressed eukaryotic cells. Previously, CDNPs isolated from cultured cells exerted immunomodulatory activities in different infections. Here, we sought to elucidate the role of CDNPs using a murine model of cecal ligation and puncture (CLP). We hypothesized that CDNPs influence the immune response at the site of infection, where severe cellular stress occurs. We observed early CDNP accumulation in the peritoneum after 4 h and continued CDNP presence 24 h after CLP...
February 22, 2017: Shock
https://www.readbyqxmd.com/read/28203235/chenopodium-ambrosioides-l-improves-phagocytic-activity-and-decreases-bacterial-growth-and-the-systemic-inflammatory-response-in-sepsis-induced-by-cecal-ligation-and-puncture
#10
Carlos E P Rios, Afonso G Abreu, Jose A F Braga Filho, Johnny R Nascimento, Rosane N M Guerra, Flávia M M Amaral, Márcia C G Maciel, Flávia R F Nascimento
Chenopodium ambrosioides L. (Amaranthaceae) is often used in different kinds of vegetal preparations for medicinal purposes in many clinical situations. Some studies have demonstrated its anti-inflammatory and immunomodulatory properties. The aim of this work was to investigate the effect of prophylactic treatment with the hydroalcoholic crude extract (HCE) of C. ambrosioides and its hexanic fraction (HEX) on the control of bacterial growth, the activation of phagocytes and the control of the systemic inflammatory response in a sepsis experimental model...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28177671/the-effect-of-short-term-oral-treatment-with-omeprazole-or-pantoprazole-on-the-function-of-polymorphonuclear-neutrophils
#11
Maja Kostrzewska, Marzena Garley, Wioletta Ratajczak, Ewa Jablonska, Jacek Jamiołkowski, Andrzej Dabrowski
Recent studies report an increased risk of enteric infections in patients treated with proton pump inhibitors (PPIs). Polymorphonuclear neutrophils (PMNs) play a key role in host response to bacterial infection. We have evaluated the effect of omeprazole and pantoprazole treatment on the PMNs function. Fifteen patients were treated with omeprazole 20 mg daily and 15 patients with pantoprazole 40 mg daily for 7 days. Treatment with omeprazole or pantoprazole had no effect on spontaneous nitroblue tetrazolium (NBT) test results...
January 22, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28176201/lactobacillus-delbrueckii-subsp-lactis-strain-cidca-133-stimulates-murine-macrophages-infected-with-citrobacter-rodentium
#12
Ayelén A Hugo, Ivanna S Rolny, David Romanin, Pablo F Pérez
Citrobacter rodentium is a specific murine enteropathogen which causes diarrheal disease characterized by colonic hyperplasia and intestinal inflammation. Recruitment of neutrophils and macrophages constitute a key step to control the infection. Since modulation of the activity of professional phagocytic cells could contribute to improve host´s defences against C. rodentium, we investigated the effect of Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) on the interaction between murine macrophages (RAW 264...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28148731/prelp-enhances-host-innate-immunity-against-the-respiratory-tract-pathogen-moraxella-catarrhalis
#13
Guanghui Liu, David Ermert, Martin E Johansson, Birendra Singh, Yu-Ching Su, Magnus Paulsson, Kristian Riesbeck, Anna M Blom
Respiratory tract infections are one of the leading causes of mortality worldwide urging better understanding of interactions between pathogens causing these infections and the host. Here we report that an extracellular matrix component proline/arginine-rich end leucine-rich repeat protein (PRELP) is a novel antibacterial component of innate immunity. We detected the presence of PRELP in human bronchoalveolar lavage fluid and showed that PRELP can be found in alveolar fluid, resident macrophages/monocytes, myofibroblasts, and the adventitia of blood vessels in lung tissue...
February 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28144894/tumor-necrosis-factor-%C3%AE-dependent-neutrophil-priming-prevents-intestinal-ischemia-reperfusion-induced-bacterial-translocation
#14
Yen-Zhen Lu, Ching-Ying Huang, Yi-Cheng Huang, Tsung-Chun Lee, Wei-Ting Kuo, Yu-Chen Pai, Linda Chia-Hui Yu
BACKGROUND: Intestinal ischemia/reperfusion (I/R) causes barrier impairment and bacterial influx. Protection against I/R injury in sterile organs by hypoxic preconditioning (HPC) had been attributed to erythropoietic and angiogenic responses. Our previous study showed attenuation of intestinal I/R injury by HPC for 21 days in a neutrophil-dependent manner. AIM: To investigate the underlying mechanisms of neutrophil priming by HPC, and explore whether adoptive transfer of primed neutrophils is sufficient to ameliorate intestinal I/R injury...
January 31, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28123633/different-virulence-of-candida-albicans-is-attributed-to-the-ability-of-escape-from-neutrophil-extracellular-traps-by-secretion-of-dnase
#15
Xiaohuan Zhang, Sainan Zhao, Luping Sun, Wenqing Li, Qiao Wei, Robert B Ashman, Yan Hu
Candida albicans is an important opportunistic fungus causing both disseminated and local infections. The discovery of neutrophil extracellular traps (NETs) has presented a new strategy to kill microorganisms in host's innate immune response. Although it has been reported that NETs can trap and kill both yeast and hyphal forms of C. albicans, the mechanism by which C. albicans escape from NETs has not been fully understood. In this study, the ability of two strains of C. albicans SC5314 and 3683 to escape NETs-mediated killing was compared...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28119468/candida-albicans-chitin-increases-arginase-1-activity-in-human-macrophages-with-an-impact-on-macrophage-antimicrobial-functions
#16
Jeanette Wagener, Donna M MacCallum, Gordon D Brown, Neil A R Gow
The opportunistic human fungal pathogen Candida albicans can cause a variety of diseases, ranging from superficial mucosal infections to life-threatening systemic infections. Phagocytic cells of the innate immune response, such as neutrophils and macrophages, are important first-line responders to an infection and generate reactive oxygen and nitrogen species as part of their protective antimicrobial response. During an infection, host cells generate nitric oxide through the enzyme inducible nitric oxide synthase (iNOS) to kill the invading pathogen...
January 24, 2017: MBio
https://www.readbyqxmd.com/read/28096329/tissue-dual-rna-seq-allows-fast-discovery-of-infection-specific-functions-and-riboregulators-shaping-host-pathogen-transcriptomes
#17
Aaron M Nuss, Michael Beckstette, Maria Pimenova, Carina Schmühl, Wiebke Opitz, Fabio Pisano, Ann Kathrin Heroven, Petra Dersch
Pathogenic bacteria need to rapidly adjust their virulence and fitness program to prevent eradication by the host. So far, underlying adaptation processes that drive pathogenesis have mostly been studied in vitro, neglecting the true complexity of host-induced stimuli acting on the invading pathogen. In this study, we developed an unbiased experimental approach that allows simultaneous monitoring of genome-wide infection-linked transcriptional alterations of the host and colonizing extracellular pathogens. Using this tool for Yersinia pseudotuberculosis-infected lymphatic tissues, we revealed numerous alterations of host transcripts associated with inflammatory and acute-phase responses, coagulative activities, and transition metal ion sequestration, highlighting that the immune response is dominated by infiltrating neutrophils and elicits a mixed TH17/TH1 response...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28095485/bordetella-parapertussis-circumvents-neutrophil-extracellular-bactericidal-mechanisms
#18
Juan Gorgojo, Emilia Scharrig, Ricardo M Gómez, Eric T Harvill, Maria Eugenia Rodríguez
B. parapertussis is a whooping cough etiological agent with the ability to evade the immune response induced by pertussis vaccines. We previously demonstrated that in the absence of opsonic antibodies B. parapertussis hampers phagocytosis by neutrophils and macrophages and, when phagocytosed, blocks intracellular killing by interfering with phagolysosomal fusion. But neutrophils can kill and/or immobilize extracellular bacteria through non-phagocytic mechanisms such as degranulation and neutrophil extracellular traps (NETs)...
2017: PloS One
https://www.readbyqxmd.com/read/28077800/oxidative-stress-induced-by-se-deficient-high-energy-diet-implicates-neutrophil-dysfunction-via-nrf2-pathway-suppression-in-swine
#19
Tianshu Yang, Zeping Zhao, Tianqi Liu, Ziwei Zhang, Pengzu Wang, Shiwen Xu, Xin Gen Len, Anshan Shan
The mechanism of the interaction between Se deficiency and high energy remains limited. The aim of the current study was to identify whether Se-deficient, high-energy diet can induce oxidative stress, and downregulate the Nrf2 pathway and phagocytic dysfunction of neutrophils. We detected the phagocytic activity, ROS production, protein levels of Nrf2 and Nrf2 downstream target genes, and the mRNA levels of 25 selenoproteins, heat shock proteins, and cytokines in neutrophils. Cytokine ELISA kits were used to measure the serum cytokines...
January 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28076241/phenotypic-and-functional-characteristics-of-hla-dr-neutrophils-in-brazilians-with-cutaneous-leishmaniasis
#20
Richard E Davis, Smriti Sharma, Jacilara Conceição, Pedro Carneiro, Fernanda Novais, Phillip Scott, Shyam Sundar, Olivia Bacellar, Edgar M Carvalho, Mary E Wilson
The protozoan Leishmania braziliensis causes cutaneous leishmaniasis (CL) in endemic regions. In murine models, neutrophils (PMNs) are recruited to the site of infection soon after parasite inoculation. However, the roles of neutrophils during chronic infection and in human disease remain undefined. We hypothesized that neutrophils help maintain a systemic inflammatory state in subjects with CL. Lesion biopsies from all patients with CL tested contained neutrophils expressing HLA-DR, a molecule thought to be restricted to professional antigen-presenting cells...
March 2017: Journal of Leukocyte Biology
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