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https://www.readbyqxmd.com/read/29777997/rumen-fluid-fermentation-for-enhancement-of-hydrolysis-and-acidification-of-grass-clipping
#1
Siqi Wang, Guangming Zhang, Panyue Zhang, Xiaowen Ma, Fan Li, Haibo Zhang, Xue Tao, Junpei Ye, Mohammad Nabi
Rumen fluid, formed in rumen of ruminants, includes a complex microbial population of bacteria, protozoa, fungi and archaea, and has high ability to degrade lignocellulosic biomass. In this study, rumen fluid was used to ferment grass clipping for enhancing the hydrolysis and acidification of organic matters. Results showed that strict anaerobic condition, higher grass clipping content and smaller particle size of grass clipping were beneficial to the hydrolysis and acidification of organics. The increase of SCOD and total VFA concentration respectively reached 24...
May 16, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29777591/bioengineering-triacetic-acid-lactone-production-in-yarrowia-lipolytica-for-pogostone-synthesis
#2
James Yu, Jenny Landberg, Farbod Shavarebi, Virginia Bilanchone, Adam Okerlund, Umayangani Wanninayake, Le Zhao, George Kraus, Suzanne Sandmeyer
Yarrowia lipolytica is an oleaginous yeast that is recognized for its ability to accumulate high levels of lipids, which can serve as precursors to biobased fuels and chemicals. Polyketides such as triacetic acid lactone (TAL) can also serve as a precursor for diverse commodity chemicals. This study used Y. lipolytica as a host organism for the production of TAL via expression of the 2-pyrone synthase gene from Gerbera hybrida. Induction of lipid biosynthesis by nitrogen-limited growth conditions increased TAL titers...
May 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29776364/identification-function-and-application-of-3-ketosteroid-%C3%AE-1-dehydrogenase-isozymes-in-mycobacterium-neoaurum-dsm-1381-for-the-production-of-steroidic-synthons
#3
Ruijie Zhang, Xiangcen Liu, Yushi Wang, Yuchang Han, Junsong Sun, Jiping Shi, Baoguo Zhang
BACKGROUND: 3-Ketosteroid-Δ1-dehydrogenase (KstD) is a key enzyme in the metabolic pathway for chemical modifications of steroid hormones. Only a few KstDs have thus far been characterized biochemically and applied for the production of steroidal pharmaceutical intermediates. Three KstDs, KstD1, KstD2, and KstD3, were identified in Mycobacterium neoaurum DSM 1381, and they shared up to 99, 85 and 97% amino acid identity with previously reported KstDs, respectively. In this paper, KstDs from M...
May 18, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29775652/advances-and-prospects-of-bacillus-subtilis-cellular-factories-from-rational-design-to-industrial-applications
#4
REVIEW
Yang Gu, Xianhao Xu, Yaokang Wu, Tengfei Niu, Yanfeng Liu, Jianghua Li, Guocheng Du, Long Liu
Bacillus subtilis is the most characterized gram-positive bacterium that has significant attributes, such as growing well on cheap carbon sources, possessing clear inherited backgrounds, having mature genetic manipulation methods, and exhibiting robustness in large-scale fermentations. Till date, B. subtilis has been identified as attractive hosts for the production of recombinant proteins and chemicals. By applying various systems and synthetic biology tools, the productivity features of B. subtilis can be thoroughly analyzed and further optimized via metabolic engineering...
May 15, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29775609/kruppel-like-factor-4-klf-4-plays-a-crucial-role-in-simvastatin-svt-induced-differentiation-of-rabbit-articular-chondrocytes
#5
Seon-Mi Yu, Song Ja Kim
Simvastatin is a cholesterol-lowing reagent that is derived synthetically from the fermentation of Aspergillus terreus. Recently, SVT has been shown to possess a protective effect of chondrocytes. Kruppel-like factor 4 (KLF-4) is a zinc finger transcription factor that plays crucial roles during the development and maintenance of multiple organs. However, the roles of KLF-4 in chondrocytes have not been well unknown. Here, we investigated whether KLF-4 regulates SVT-caused differentiated phenotype of chondrocytes...
May 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29775544/recovery-of-glucose-from-dried-distiller-s-grain-with-solubles-using-combinations-of-solid-state-fermentation-and-insect-culture
#6
Timothy Howdeshell, Takuji Tanaka
A bioethanol byproduct (dried distiller's grains with solubles; DDGS) contains a high cellulose and starch. We hypothesized combinations of solid-state fermentation (SSF) and digestion by black soldier fly larvae (BSFL) (Hermetia illucens) could increase the recovery of glucose from this byproduct through concentrating and loosening cellulose matrix by their activities. DDGS was individually fermented with Aspergillus niger, Aspergillus fumigatus, Trichoderma koningii, Phanerochaete chrysosporium, or Lactobacillus plantarum...
May 18, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29774508/the-bacterial-and-archaeal-community-structures-and-methanogenic-potential-of-the-cecal-microbiota-of-goats-fed-with-hay-and-high-grain-diets
#7
Wei Jin, Yin Li, Yanfen Cheng, Shengyong Mao, Weiyun Zhu
The cecum plays an important role in the feed fermentation of ruminants. However, information is very limited regarding the cecal microbiota and their methane production. In the present study, the cecal content from twelve local Chinese goats, fed with either a hay diet (0% grain) or a high-grain diet (71.5% grain), were used to investigate the bacterial and archaeal community and their methanogenic potential. Microbial community analysis was determined using high-throughput sequencing of 16S rRNA genes and real-time PCR, and the methanogenesis potential was assessed by in vitro fermentation with ground corn or hay as substrates...
May 17, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29773621/genome-sequence-of-the-self-flocculating-strain-saccharomyces-cerevisiae-spsc01
#8
Jian-Ren Xu, Lei-Yu He, Chen-Guang Liu, Xin-Qing Zhao, Feng-Wu Bai
The self-flocculation of yeast cells presents advantages for continuous ethanol fermentation such as their self-immobilization within fermenters for high density to improve ethanol productivity and cost-effective biomass recovery by gravity sedimentation. We sequenced and analyzed the genome of the self-flocculating Saccharomyces cerevisiae SPSC01 for the industrial production of fuel ethanol.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29773074/enhanced-cellulase-production-in-trichoderma-reesei-rut-c30-via-constitution-of-minimal-transcriptional-activators
#9
Jiajia Zhang, Guoxiu Zhang, Wei Wang, Wei Wang, Dongzhi Wei
BACKGROUND: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. RESULTS: Two sets of novel minimal transcriptional activators (DBDace2 -VP16 and DBDcre1 -VP16) were designed and expressed in T...
May 17, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29772827/physicochemical-nutritional-and-organoleptic-characterization-of-a-skimmed-goat-milk-fermented-with-the-probiotic-strain-lactobacillus-plantarum-c4
#10
Miriam Moreno-Montoro, Miguel Navarro-Alarcón, Triana Bergillos-Meca, Rafael Giménez-Martínez, Silvia Sánchez-Hernández, Manuel Olalla-Herrera
The benefits of goat milk, fermented milks, and probiotics for the humans are well documented. In this study, a novel fermented goat milk was manufactured with the putative probiotic strain Lactobacillus plantarum C4 together with L. bulgaricus and Streptococcus thermophilus . Ultrafiltration was chosen as the skimmed milk concentration method because it produced the best viscosity and syneresis and a high casein content. The viability rate of all bacterial strains was >10⁷ cfu/mL, even after 5 weeks of storage or after in vitro gastrointestinal digestion, which is especially important for exertion of the probiotic strain functionalities...
May 17, 2018: Nutrients
https://www.readbyqxmd.com/read/29772563/assessment-of-different-pretreatment-technologies-for-efficient-bioconversion-of-lignocellulose-to-ethanol
#11
Jitendra K Singh, Preeti Vyas, Anamika Dubey, Chandrama Prakash Upadhyaya, Richa Kothari, Vineet Veer Tyagi, Ashwani Kumar
The future supply of energy to meet growing energy demand of rapidly exapanding populations is based on wide energy resources, particularly the renewable ones. Among all resources, lignocellulosic biomasses such as agriculture, forest, and agro-industrial residues are the most abundant and easily available bioresource for biorefineries to provide fuels, chemicals, and materials. However, pretreatment of biomass is required to overcome the physical and chemical barriers that exist in the lignin-carbohydrate composite and pretreatment facilitate the entry of biocatalysts for the conversion of biomass into fermentable sugars and other by-products...
June 1, 2018: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/29772505/enhancing-fermentative-hydrogen-production-with-the-removal-of-volatile-fatty-acids-by-electrodialysis
#12
Pengfei Wei, Ao Xia, Qiang Liao, Chihe Sun, Yun Huang, Qian Fu, Xun Zhu, Richen Lin
A three-chamber electrodialysis bioreactor comprising fermentation, cathode and anode chambers was proposed to remove in situ volatile fatty acids during hydrogen fermentation. The electrodialysis voltage of 4 V resulted in a volumetric hydrogen productivity of 1878.0 mL/L from the fermentation chamber, which is 55.4% higher than that (1208.5 mL/L) of the control group without voltage applied. Gas production was not observed in the cathode and anode chambers throughout fermentation. By applying different voltages (0-6 V), the hydrogen content accumulated to 54...
May 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29772465/effect-of-temperature-on-production-of-oligosaccharides-and-dextran-by-weissella-cibaria-10-m
#13
Ying Hu, Michael G Gänzle
The formation of HoPS and oligosaccharides in sourdough fermentation improves bread quality but is dependent on the expression of glycansucrases by lactic acid bacteria. Data on the expression of dextransucrases by Weissella spp., however, are limited. This study therefore aimed to assess dextansucrase expression in W. cibaria 10 M, focusing on the effect of temperature. The effect of temperature on growth, oligosaccharide and dextran synthesis by W. cibaria 10 M was determined and the expression and activity of cell-associated dextransucrase from W...
May 8, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29772177/capture-ferment-upgrade-a-three-step-approach-for-the-valorization-of-sewage-organics-as-commodities
#14
Abbas Alloul, Ramon Ganigue, Marc Spiller, Francis A Meerburg, Cristina Cagnetta, Korneel Rabaey, Siegfried E Vlaeminck
This critical review outlines a roadmap for the conversion of chemical oxygen demand (COD) contained in sewage to commodities based on three-steps: capture COD as sludge, ferment it to volatile fatty acids (VFA), and upgrade VFA to products. The article analyzes the state-of-the-art of this three-step approach and discusses the bottlenecks and challenges. The potential of this approach is illustrated for the European Union's 28 member states (EU-28) through Monte Carlo simulations. High-rate contact stabilization captures the highest amount of COD (66-86 gCOD person equivalent-1 day-1 in 60% of the iterations)...
May 17, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29771392/carcass-characteristics-and-meat-quality-of-broilers-fed-with-different-levels-of-saccharomyces-cerevisiae-fermentation-product
#15
L G A Aristides, E J Venancio, A A Alfieri, R A A Otonel, W J Frank, A Oba
Fermented products and components of Saccharomyces cerevisiae have been widely used in animal nutrition to promote the development and quality of broilers. This study aims to evaluate different levels of inclusion (0, 250, 750, 1,500 g/t) of S. cerevisiae fermentation product (SCFP) in broiler feed to gauge its effect on carcass characteristics and cuts beyond the quality of breast meat. For analyses of carcass yield, cuts, and meat quality, 16 broilers per treatment were slaughtered. The meat quality analyses were performed 24 h after slaughter and evaluated color, pH, water holding capacity, cooking loss, and shear force...
May 16, 2018: Poultry Science
https://www.readbyqxmd.com/read/29771352/de-novo-biosynthesis-of-anthocyanins-in-saccharomyces-cerevisiae
#16
Michael Eichenberger, Anders Hansson, David Fischer, Lara Dürr, Michael Naesby
Anthocyanins (ACNs) are plant secondary metabolites responsible for most of the red, purple and blue colors of flowers, fruits and vegetables. They are increasingly used in the food and beverage industry as natural alternative to artificial colorants. Production of these compounds by fermentation of microorganisms would provide an attractive alternative. In this study, Saccharomyces cerevisiae was engineered for de novo production of the three basic anthocyanins, as well as the three main trans-flavan-3-ols...
June 1, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29771304/laboratory-evolution-for-forced-glucose-xylose-co-consumption-enables-identification-of-mutations-that-improve-mixed-sugar-fermentation-by-xylose-fermenting-saccharomyces-cerevisiae
#17
Ioannis Papapetridis, Maarten D Verhoeven, Sanne J Wiersma, Maaike Goudriaan, Antonius J A van Maris, Jack T Pronk
Simultaneous fermentation of glucose and xylose can contribute to improved productivity and robustness of yeast-based processes for bioethanol production from lignocellulosic hydrolysates. This study explores a novel laboratory evolution strategy for identifying mutations that contribute to simultaneous utilization of these sugars in batch cultures of Saccharomyces cerevisiae. To force simultaneous utilization of xylose and glucose, the genes encoding glucose-6-phosphate isomerase (PGI1) and ribulose-5-phosphate epimerase (RPE1) were deleted in a xylose-isomerase-based xylose-fermenting strain with a modified oxidative pentose-phosphate pathway...
May 15, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29770550/improved-robustness-of-an-ethanologenic-yeast-strain-through-adaptive-evolution-in-acetic-acid-is-associated-with-its-enzymatic-antioxidant-ability
#18
N Gurdo, G F Novelli Poisson, Á B Juárez, M C Ríos de Molina, M A Galvagno
AIMS: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. METHODS AND RESULTS: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l-1 ) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles...
May 16, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29769717/swi2-snf2-atpase-chr2-remodels-pri-mirnas-via-serrate-to-impede-mirna-production
#19
Zhiye Wang, Zeyang Ma, Claudia Castillo-González, Di Sun, Yanjun Li, Bin Yu, Baoyu Zhao, Pingwei Li, Xiuren Zhang
Chromatin remodelling factors (CHRs) typically function to alter chromatin structure. CHRs also reside in ribonucleoprotein complexes, but little is known about their RNA-related functions. Here we show that CHR2 (also known as BRM), the ATPase subunit of the large switch/sucrose non-fermentable (SWI/SNF) complex, is a partner of the Microprocessor component Serrate (SE). CHR2 promotes the transcription of primary microRNA precursors (pri-miRNAs) while repressing miRNA accumulation in vivo. Direct interaction with SE is required for post-transcriptional inhibition of miRNA accumulation by CHR2 but not for its transcriptional activity...
May 16, 2018: Nature
https://www.readbyqxmd.com/read/29769378/food-starch-structure-impacts-gut-microbiome-composition
#20
Frederick J Warren, Naoki M Fukuma, Deirdre Mikkelsen, Bernadine M Flanagan, Barbara A Williams, Allan T Lisle, Páraic Ó Cuív, Mark Morrison, Michael J Gidley
Starch is a major source of energy in the human diet and is consumed in diverse forms. Resistant starch (RS) escapes small intestinal digestion and is fermented in the colon by the resident microbiota, with beneficial impacts on colonic function and host health, but the impacts of the micro- and nanoscale structure of different physical forms of food starch on the broader microbial community have not been described previously. Here, we use a porcine in vitro fermentation model to establish that starch structure dramatically impacts microbiome composition, including the key amylolytic species, and markedly alters both digestion kinetics and fermentation outcomes...
June 27, 2018: MSphere
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