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https://www.readbyqxmd.com/read/28441744/production-of-laccase-by-a-new-myrothecium-verrucaria-md-r-16-isolated-from-pigeon-pea-cajanus-cajan-l-millsp-and-its-application-on-dye-decolorization
#1
Jiao Sun, Na Guo, Li-Li Niu, Qing-Fang Wang, Yu-Ping Zang, Yuan-Gang Zu, Yu-Jie Fu
The present study was conducted to screen a laccase-producing fungal endophyte, optimize fermentation conditions, and evaluate the decolorization ability of the laccase. A new fungal endophyte capable of laccase-producing was firstly isolated from pigeon pea and identified as Myrothecium verrucaria based on a ITS-rRNA sequences analysis. Meanwhile, various fermentation parameters on the laccase production were optimized via response surface methodology (RSM). The optimal fermentation conditions were a fermentation time of five days, temperature 30 °C and pH 6...
April 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28440871/effects-of-different-salt-treatments-on-the-fermentation-metabolites-and-bacterial-profiles-of-kimchi
#2
Dong Wook Kim, Bo-Min Kim, Hyeon-Jeong Lee, Gwang-Ju Jang, Seong Hwa Song, Jae-In Lee, Sang Bong Lee, Jae Min Shim, Kang Wook Lee, Jeong Hwan Kim, Kyung-Sik Ham, Feng Chen, Hyun-Jin Kim
The effects of purified salt (PS) and mineral-rich sea salt (MRS), both with different mineral profiles, on kimchi fermentation were studied using a culture-dependent 16S rRNA sequencing technique and mass-based metabolomic analysis. The different mineral profiles in the fermentation medium caused changes in the bacterial profiles of the 2 kimchi products. An increase of Leuconostoc species in MRS-kimchi decreased the Lactobacillus/Leuconostoc ratio, which led to changes in metabolites (including sugars, amino acids, organic acids, lipids, sulfur compounds, and terpenoids) associated with kimchi quality...
April 25, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28440059/production-of-itaconic-acid-from-pentose-sugars-by-aspergillus-terreus
#3
Badal C Saha, Gregory J Kennedy, Nasib Qureshi, Michael J Bowman
Itaconic acid (IA), an unsaturated 5-carbon dicarboxylic acid, is a building block platform chemical that is currently produced industrially from glucose by fermentation with Aspergillus terreus. However, lignocellulosic biomass has potential to serve as low cost source of sugars for production of IA. Research needs to be performed to find a suitable A. terreus strain that can use lignocellulose derived pentose sugars and produce IA. One hundred A. terreus strains were evaluated for the first time for production of IA from xylose and arabinose...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28439766/enhanced-butyric-acid-tolerance-and-production-by-class-i-heat-shock-protein-overproducing-clostridium-tyrobutyricum-atcc-25755
#4
Yukai Suo, Sheng Luo, Yanan Zhang, Zhengping Liao, Jufang Wang
The response of Clostridium tyrobutyricum to butyric acid stress involves various stress-related genes, and therefore overexpression of stress-related genes can improve butyric acid tolerance and yield. Class I heat shock proteins (HSPs) play an important role in the process of protecting bacteria from sudden changes of extracellular stress by assisting protein folding correctly. The results of quantitative real-time PCR indicated that the Class I HSGs grpE, dnaK, dnaJ, groEL, groES, and htpG were significantly upregulated under butyric acid stress, especially the dnaK and groE operons...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439463/molecular-assessment-of-the-fecal-microbiota-in-healthy-cats-and-dogs-before-and-during-supplementation-with-fructo-oligosaccharides-fos-and-inulin-using-high-throughput-454-pyrosequencing
#5
Jose F Garcia-Mazcorro, Jose R Barcenas-Walls, Jan S Suchodolski, Jörg M Steiner
Prebiotics are selectively fermentable dietary compounds that result in changes in the composition and/or activity of the intestinal microbiota, thus conferring benefits upon host health. In veterinary medicine, commercially available products containing prebiotics have not been well studied with regard to the changes they trigger on the composition of the gut microbiota. This study evaluated the effect of a commercially available nutraceutical containing fructo-oligosaccharides (FOS) and inulin on the fecal microbiota of healthy cats and dogs when administered for 16 days...
2017: PeerJ
https://www.readbyqxmd.com/read/28439295/production-of-high-concentration-n-caproic-acid-from-lactate-through-fermentation-using-a-newly-isolated-ruminococcaceae-bacterium-cpb6
#6
Xiaoyu Zhu, Yan Zhou, Yi Wang, Tingting Wu, Xiangzhen Li, Daping Li, Yong Tao
BACKGROUND: n-Caproic acid (CA), as a medium-chain carboxylic acid, is a valuable chemical feedstock for various industrial applications. The fermentative production of CA from renewable carbon sources has attracted a lot of attentions. Lactate is a significant intermediate waste in the anaerobic breakdown of carbohydrates that comprise 18-70% of the chemical oxygen demand (COD) in municipal and some industrial wastewaters. Recently, researchers (including our own group) reported the CA production using lactate as electron donor with newly identified microbiome systems...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28438359/impact-of-selectively-enriched-microbial-communities-on-long-term-fermentative-biohydrogen-production
#7
R Kanniah Goud, Kotakonda Arunasri, Dileep Kumar Yeruva, K Vamshi Krishna, Shikha Dahiya, S Venkata Mohan
The effect of selectively enriched inoculum for biohydrogen production and shifts in microbial communities was observed using anaerobic sequencing batch reactor (AnSBR). Significantly, combined pretreatment using acid and iodopropane resulted 3 fold increase in H2 production (8.65mol/kgCODR) over untreated control (2.63mol/kgCODR). Pretreatment showed significant shifts in the microbial communities which are thus accounted for enhanced H2 production. The high-throughput pyrosequence analysis depicted shifts in phylum Firmicutes and Proteobacteria...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28438358/potential-of-oleaginous-yeast-trichosporon-sp-for-conversion-of-sugarcane-bagasse-hydrolysate-into-biodiesel
#8
K K Brar, A K Sarma, Mohammad Aslam, Igor Polikarpov, B S Chadha
This study reports production of microbial oil from a yeast strain Trichosporon sp., (RW) isolated from decayed wood. Preliminary analysis based on fluorescence microscopy and spectroscopy of Nile red stained yeast cells showed accumulation of lipid globules. The potential of the yeast to produce lipids was evaluated on glucose, glycerol and acid hydrolysate of sugarcane bagasse, where Trichosporon sp. (RW) was found to accumulate 21.45 (59.6%), 18.41 (56%) and 10.25g/l (40.5%) of the lipids after 120h of fermentation at 30°C...
March 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28438014/characterization-of-a-glutamate-decarboxylase-gad-from-enterococcus-avium-m5-isolated-from-jeotgal-a-fermented-korean-sea-food
#9
Kang Wook Lee, Jae Min Shim, Zhuang Yao, Jeong A Kim, Hyun-Jin Kim, Jeong Hwan Kim
To develop starters for the production of functional foods or materials, lactic acid bacteria producing γ-aminobutyric acid (GABA) were screened from jeotgals, Korean fermented sea foods. One isolate producing high amount of GABA from monosodium L-glutamate (MSG) was identified as Enterococcus avium by 16S rRNA gene sequencing. E. avium M5 produced 18.47 ± 1.26 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with MSG (3%, w/v). A gadB gene encoding glutamate decarboxylase (GAD) was cloned and overexpressed in E...
April 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28437455/effect-of-a-probiotic-beverage-consumption-enterococcus-faecium-crl-183-and-bifidobacterium-longum-atcc-15707-in-rats-with-chemically-induced-colitis
#10
Larissa Sbaglia Celiberto, Raquel Bedani, Naiara Naiana Dejani, Alexandra Ivo de Medeiros, José Antonio Sampaio Zuanon, Luis Carlos Spolidorio, Maria Angela Tallarico Adorno, Maria Bernadete Amâncio Varesche, Fábio Carrilho Galvão, Sandro Roberto Valentini, Graciela Font de Valdez, Elizeu Antonio Rossi, Daniela Cardoso Umbelino Cavallini
BACKGROUND: Some probiotic strains have the potential to assist in relieving the symptoms of inflammatory bowel disease. The impact of daily ingestion of a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within a period of 30 days. METHODS: Colitis was induced by dextran sulfate sodium. The animals were randomly assigned into five groups: Group C: negative control; Group CL: positive control; Group CLF: DSS with the fermented product; Group CLP: DSS with the non-fermented product (placebo); Group CLS: DSS with sulfasalazine...
2017: PloS One
https://www.readbyqxmd.com/read/28437452/enhanced-antibacterial-metabolite-production-through-the-application-of-statistical-methodologies-by-a-streptomyces-nogalater-niist-a30-isolated-from-western-ghats-forest-soil
#11
Jubi Jacob, Reshma Uma Rajendran, Syama Hari Priya, Jayamurthy Purushothaman, Dileep Kumar Bhaskaran Nair Saraswathy Amma
Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial properties and were further screened against eleven bacterial pathogens. A strain identified as Streptomyces nogalater and designated as NIIST A30 exhibited maximum inhibition against all the test pathogens. Among the eight fermentation media tested, inorganic salts starch broth recorded the best for antibacterial production...
2017: PloS One
https://www.readbyqxmd.com/read/28436828/sensory-profile-and-volatile-aroma-composition-of-reduced-alcohol-merlot-wines-fermented-with-metschnikowia-pulcherrima-and-saccharomyces-uvarum
#12
C Varela, A Barker, T Tran, A Borneman, C Curtin
Strategies for production of wines containing lower alcohol concentrations are in strong demand, for reasons of quality, health, and taxation. Development and application of wine yeasts that are less efficient at transforming grape sugars into ethanol has the potential to allow winemakers the freedom to make lower alcohol wines from grapes harvested at optimal ripeness, without the need for post-fermentation processes aimed at removing ethanol. We have recently shown that two non-conventional wine yeast species Metschnikowia pulcherrima and Saccharomyces uvarum were both able to produce wine with reduced alcohol concentration...
April 12, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28436165/production-of-high-concentration-of-l-lactic-acid-from-oil-palm-empty-fruit-bunch-by-thermophilic-b-coagulans-ji12
#13
Veeresh Juturu, Jin Chuan Wu
Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch (EFB) at 50°C and pH6, producing 105.4 g/L of L-lactic acid with a productivity of 9.3 g/L/h by fed batch fermentation under unsterilized conditions. Simultaneous saccharification and fermentation (SSF) was performed at pH5.5 and 50°C to convert both hemicellulose hydrolysate and cellulose-lignin complex in the presence of Cellic Ctec2 cellulases using yeast extract (20 g/L) as the nitrogen source, giving 114...
April 24, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28434727/short-term-effects-of-soybean-oil-supplementation-on-performance-digestion-and-metabolism-in-dairy-cows-fed-sugarcane-based-diets
#14
João Paulo P Rodrigues, Ricardo M de Paula, Luciana N Rennó, Marta M S Fontes, Andreia F Machado, Sebastião de C Valadares Filho, Pekka Huhtanen, Marcos I Marcondes
We aimed to quantify the productive and metabolic responses, and digestive changes in dairy cows fed various concentrations of soybean oil (SBO) in high-concentrate, sugarcane-based diets. Eight rumen-cannulated multiparous Holstein cows in mid lactation (574 ± 19.1 kg of body weight and 122 ± 6.9 d in milk), averaging 22.5 ± 1.22 kg/d of milk were assigned to replicated 4 × 4 Latin squares. The experimental period lasted 21 d as follows: 14 d for adaptation, followed by a sampling period from d 15 to 21...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434726/short-communication-sensory-analysis-of-a-kefir-product-designed-for-active-cancer-survivors
#15
K O'Brien, C Boeneke, W Prinyawiwatkul, J Lisano, D Shackelford, K Reeves, M Christensen, R Hayward, K Carabante Ordonez, L K Stewart
Kefir is a fermented milk product that is a good source of protein and health-promoting bacteria. It has the potential to improve recovery from exercise, and the health and well-being of cancer survivors. The purpose of this study was to explore cancer survivor attitudes about and acceptance of a kefir recovery beverage made from cultured milk, whole fruit, natural sweeteners, and other natural ingredients. Kefir was made by inoculating and fermenting milk with kefir grains. The kefir was then mixed with a fruit base and given to cancer survivors (n = 52) following a bout of exercise...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434721/characterization-of-probiotic-bacteria-involved-in-fermented-milk-processing-enriched-with-folic-acid
#16
Zhen Wu, Jing Wu, Pei Cao, Yifeng Jin, Daodong Pan, Xiaoqun Zeng, Yuxing Guo
Yogurt products fermented with probiotic bacteria are a consumer trend and a challenge for functional food development. So far, limited research has focused on the behavior of the various probiotic strains used in milk fermentation. In the present study, we characterized folic acid production and the sensory and textural characteristics of yogurt products fermented with probiotic bacteria. Yogurt fermented with Lactobacillus plantarum had improved nutrient content and sensory and textural characteristics, but the presence of L...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28433914/accumulation-of-sugar-from-pulp-and-xylitol-from-xylose-by-pyruvate-decarboxylase-negative-white-rot-fungus-phlebia-sp-mg-60
#17
Taku Tsuyama, Megumi Yamaguchi, Ichiro Kamei
Phlebia sp. MG-60 is a white-rot fungus that produces ethanol with high efficiency from lignocellulosic biomass without additional enzymes. Through engineering of this powerful metabolic pathway for fermentation in Phlebia sp. MG-60, chemical compounds other than ethanol could be produced. Here, we demonstrate sugar accumulation from unbleached hardwood kraft pulp and conversion of xylose to xylitol by pyruvate decarboxylase (pdc)-negative Phlebia sp. MG-60. We isolated Phlebia sp. strain MG-60-P2 from protoplasts to unify the protoplast phenotypes of the regenerated strains...
April 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433910/succinic-acid-production-by-immobilized-cultures-using-spent-sulphite-liquor-as-fermentation-medium
#18
Maria Alexandri, Harris Papapostolou, Lutgart Stragier, Willy Verstraete, Seraphim Papanikolaou, Apostolis A Koutinas
Spent sulphite liquor (SSL) was used as carbon source for the production of succinic acid using immobilized cultures of Actinobacillus succinogenes and Basfia succiniciproducens on two different supports, delignified cellulosic material (DCM) and alginate beads. Fed-batch immobilized cultures with A. succinogenes in alginates resulted in higher sugar to succinic acid conversion yield (0.81g/g) than the respective yield achieved (0.65g/g) when DCM immobilized cultures were used. The final succinic acid concentration and yield achieved in fed-batch with immobilized cultures of B...
March 25, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433905/pretreating-wheat-straw-by-phosphoric-acid-plus-hydrogen-peroxide-for-enzymatic-saccharification-and-ethanol-production-at-high-solid-loading
#19
Jingwen Qiu, Lunjie Ma, Fei Shen, Gang Yang, Yanzong Zhang, Shihuai Deng, Jing Zhang, Yongmei Zeng, Yaodong Hu
Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP) for enzymatic hydrolysis and ethanol fermentation at high solid loadings. Results indicated solid loading could reach 20% with 77.4% cellulose-glucose conversion and glucose concentration of 164.9g/L in hydrolysate, it even was promoted to 25% with only 3.4% decrease on cellulose-glucose conversion as the pretreated-wheat straw was dewatered by air-drying. 72.9% cellulose-glucose conversion still was achieved as the minimized enzyme input of 20mg protein/g cellulose was employed for hydrolysis at 20% solid loading...
April 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433585/scale-up-for-esters-production-from-straw-whiskers-for-biofuel-applications
#20
Agapi Dima, Konstantina Boura, Argyro Bekatorou, Panagiota-Yiolanda Stergiou, Athanasios Foukis, Olga A Gkini, Panagiotis Kandylis, Katerina Pissaridi, Maria Kanellaki, Emmanuel M Papamichael, Athanasios A Koutinas
Delignified wheat straw was fermented by a mixed bacterial anaerobic culture obtained from a UASB reactor to produce organic acids (OAs). Kissiris was used as immobilization carrier in a 2-compartment 82L bioreactor filled with 17L of fermentation broth for the first 7 fermentation batches and up to 40L for the subsequent batches. The amount of straw used was 30g/L and the temperature was set at 37°C for all experiments. The total OAs reached concentrations up to 17.53g/L and the produced ethanol ranged from 0...
April 9, 2017: Bioresource Technology
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