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https://www.readbyqxmd.com/read/29151160/tolerance-improvement-of-corynebacterium-glutamicum-on-lignocellulose-derived-inhibitors-by-adaptive-evolution
#1
Xia Wang, Imrana Khushk, Yanqiu Xiao, Qiuqiang Gao, Jie Bao
Robustness of fermenting strains to lignocellulose derived inhibitors is critical for efficient biofuel and biochemical productions. In this study, the industrial fermenting strain Corynebacterium glutamicum S9114 was evolved for improved inhibitor tolerance using long-term adaptive evolution by continuously transferring into the inhibitors containing corn stover hydrolysate every 24 h, and finally a stably evolved C. glutamicum was obtained after 128 days of serial transfers. The evolved strain exhibited the highly increased conversion rate to the typical lignocellulose derived inhibitors including furfural, 5-hydroxymethylfurfural, vanillin, syringaldehyde, 4-hydroxybenzaldehyde, and acetic acid...
November 18, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29150805/aspergillus-terreus-treated-rice-straw-suppresses-methane-production-and-enhances-feed-digestibility-in-goats
#2
P Mohd Azlan, M F Jahromi, M O Ariff, M Ebrahimi, S C L Candyrine, J B Liang
The objectives of this study were to test the efficacy of producing lovastatin in rice straw treated with Aspergillus terreus in larger laboratory scale following the procedure previously reported and to investigate the effectiveness of the treated rice straw containing lovastatin on methane mitigation in goats. The concentration of lovastatin in the treated rice straw was 0.69 ± 0.05 g/kg dry matter (DM) rice straw. Our results showed that supplementation of lovastatin at 4.14 mg/kg BW reduced methane production by 32% while improving the DM digestibility by 13% (P < 0...
November 17, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29150774/co-expression-of-exo-inulinase-and-endo-inulinase-genes-in-the-oleaginous-yeast-yarrowia-lipolytica-for-efficient-single-cell-oil-production-from-inulin
#3
Nianci Shi, Weian Mao, Xiaoxia He, Zhe Chi, Zhenming Chi, Guanglei Liu
Yarrowia lipolytica is a promising platform for the single cell oil (SCO) production. In this study, a transformant X+N8 in which exo- and endo-inulinase genes were co-expressed could produce an inulinase activity of 124.33 U/mL within 72 h. However, the inulinase activity of a transformant X2 carrying a single exo-inulinase gene was only 47.33 U/mL within 72 h. Moreover, the transformant X+N8 could accumulate 48.13% (w/w) SCO from inulin and the cell dry weight reached 13.63 g/L within 78 h, which were significantly higher than those of the transformant X2 (41...
November 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29150754/detoxification-of-aflatoxin-b1-by-antifungal-compounds-from-lactobacillus-brevis-and-lactobacillus-paracasei-isolated-from-dairy-products
#4
Eman Zakaria Gomaa, Manal Farouk Abdelall, Omima Mohammed El-Mahdy
Aflatoxins are a large group of highly toxic, mutagenic, and carcinogenic mycotoxins produced by specific species of fungi. Potential contamination of food commodities by these compounds causes extensive damage that lead to great economic losses. This study explored the potential use of antifungal compounds, produced by Lactobacillus brevis and Lactobacillus paracasei, for growth inhibition and subsequent aflatoxin B1 production from select strains of Aspergillus flavus and Aspergillus parasiticus. Lactobacilli strains were isolated from traditional Egyptian dairy products, whereas fungal strains were isolated from infected cereal seeds...
November 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29150708/production-of-arabitol-from-enzymatic-hydrolysate-of-soybean-flour-by-debaryomyces-hansenii-fermentation
#5
Abdullah A Loman, S M M Islam, Lu-Kwang Ju
Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Effects of medium composition, operating conditions, and culture stage (growing or stationary phase) were studied. Production was also compared at different culture volumes to understand the effect of dissolved oxygen concentration (DO)...
November 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29150701/process-development-of-oxalic-acid-production-in-submerged-culture-of-aspergillus-niger-f22-and-its-biocontrol-efficacy-against-the-root-knot-nematode-meloidogyne-incognita
#6
Sang Il Lee, Keon Jin Lee, Ho Hyun Chun, Sanghyun Ha, Hyun Jung Gwak, Ho Myeong Kim, Jong-Hee Lee, Hak-Jong Choi, Hyeong Hwan Kim, Teak Soo Shin, Hae Woong Park, Jin-Cheol Kim
Oxalic acid has potent nematicidal activity against the root-knot nematode Meloidogyne incognita. In this study, fermentation parameters for oxalic acid production in submerged culture of Aspergillus niger F22 at 23, 25, and 30 °C were optimized in 5-L jar fermenters. The viscosity of the culture broth increased with increasing temperature. There was a negative correlation between oxalic acid production and the apparent viscosity; high volumetric productivity of oxalic acid was obtained at low apparent viscosity (less than 1000 cP), with a productivity of more than 100 mg/L h...
November 17, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29150509/the-tape-measure-protein-is-involved-in-the-heat-stability-of-lactococcus-lactis-phages
#7
Hany Geagea, Simon J Labrie, Muriel Subirade, Sylvain Moineau
Virulent lactococcal phages are still a major risk for milk fermentation processes as they may lead to slowdowns and low quality fermented dairy products, particularly cheeses. Some of the phage control strategies used by the industry rely on heat treatments. Recently, a few Lactococcus lactis phages were found to be highly thermo-resistant. To identify the genetic determinant(s) responsible for the thermal resistance of lactococcal phages, we used the virulent phage CB14 (sk1virus/936 group) to select for phage mutants with increased heat stability...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29149843/characterization-of-a-novel-bioflocculant-from-a-marine-bacterium-and-its-application-in-dye-wastewater-treatment
#8
Zhen Chen, Zhipeng Li, Peize Liu, Yu Liu, Yuanpeng Wang, Qingbiao Li, Ning He
BACKGROUND: The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screening of marine bacteria, and we report for the first time the properties of the bioflocculant produced by Alteromonas sp. in the treatment of dye wastewater. RESULTS: A bioflocculant-producing bacterium was isolated from seawater and identified as Alteromonas sp...
November 17, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29149547/engineering-escherichia-coli-co-cultures-for-production-of-curcuminoids-from-glucose
#9
Zhen Fang, J Andrew Jones, Jingwen Zhou, Mattheos A G Koffas
Curcuminoids have attracted increasing attention because of the antioxidant, anticancer, and antitumor activities while their production is limited because of its main source, turmeric plant, demonstrates extensive seasonal variation. In this study, we constructed Escherichia coli co-culture system for the rapid production of curcuminoids from glucose. Firstly, the overexpression of curcuminoid synthase and four different strategies related to increasing the intracellular malonyl-CoA pool were conducted in engineered E...
November 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29149220/a-novel-method-to-rapidly-distinguish-the-geographical-origin-of-traditional-fermented-salted-vegetables-by-mass-fingerprinting
#10
So-Ra Yoon, Sung Hyun Kim, Hae-Won Lee, Ji-Hyoung Ha
The geographical origin of kimchi is of interest to consumers and producers because the prices of commercial kimchi products can vary significantly according to the geographical origin. Hence, social issues related to the geographical origin of kimchi in Korea have emerged as a major problem. In this study, the geographical origin of kimchi was determined by comparing the mass fingerprints obtained for Korean and Chinese kimchi samples by MALDI-TOF MS with multivariate analysis. The results obtained herein provide an accurate, powerful tool to clearly discriminate kimchi samples based on their geographical origin within a short time and to ensure food authenticity, which is of significance in the kimchi industry...
2017: PloS One
https://www.readbyqxmd.com/read/29147976/evaluation-of-microbial-consortia-and-chemical-changes-in-spontaneous-maize-bran-fermentation
#11
Marilù Decimo, Mattia Quattrini, Giovanni Ricci, Maria Grazia Fortina, Milena Brasca, Tiziana Silvetti, Federica Manini, Daniela Erba, Franca Criscuoli, Maria Cristina Casiraghi
Sustainable exploitation of agro-industrial by-products has attracted great interest in cereal bran valorization. In this research, a polyphasic approach has been carried out to characterize maize bran at microbiological and chemical level during a sourdough like fermentation process, in order to enhance its technological and nutritional properties. Autochthonous microbiota was isolated at different refreshment steps and subjected to identification and molecular characterization. Fermentation was characterized by a rapid increase in lactic acid bacteria and yeasts, with a co-dominance, at the initial stage, of Weissella spp...
November 16, 2017: AMB Express
https://www.readbyqxmd.com/read/29147103/antibacterial-effects-against-various-foodborne-pathogens-and-sensory-properties-of-yogurt-supplemented-with-panax-ginseng-marc-extract
#12
Su Jin Eom, Ji Eun Hwang, Kee-Tae Kim, Hyun-Dong Paik
Panax ginseng marc is produced from fresh ginseng roots during processing and is generally treated as industrial waste. The primary aim of this study was to improve its utilization in the dairy industry as a potential high-value resource. Yogurt was prepared from 11% skim milk powder, 0.1% pectin, 10% sucrose, and ginseng marc ethanol extract (GME, 0.5% and 1.0%) in milk, and was inoculated with a 0.02% yogurt culture (Lactobacillus acidophilus, Bifidobacterium longum, and Streptococcus thermophilus). After fermentation at 40°C for 6-8 h, the physicochemical properties of samples were analyzed by the AOAC, Kjeldahl, and Soxhlet methods...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29147097/characterization-of-paenibacillus-sp-mbt213-isolated-from-raw-milk-and-its-ability-to-convert-ginsenoside-rb1-into-ginsenoside-rd-from-panax-ginseng
#13
Gereltuya Renchinkhand, Soo Hyun Cho, Magsar Urgamal, Young W Park, Joong Hyeon Nam, Hyung Churl Bae, Gyu Yong Song, Myoung Soo Nam
This study was conducted to isolate and characterize Paenibacillus sp. MBT213 possessing β-glucosidase activity from raw milk, and examine the enzymatic capacity on the hydrolysis of a major ginsenoside (Rb1). Strain MBT213 was found to have a high hydrolytic ability on ginsenoside Rb1 by Esculin Iron Agar test. 16S rDNA analysis revealed that MBT213 was Paenibacillu sp. Crude enzyme of MBT213 strain exhibited high conversion capacity on ginsenoside Rb1 into ginsenoside Rd proven by TLC and HPLC analyses. The API ZYM kit confirmed that Paenibacillu sp...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29146927/preparation-and-functional-evaluation-of-collagen-oligopeptide-rich-hydrolysate-from-fish-skin-with-the-serine-collagenolytic-protease-from-pseudoalteromonas-sp-sm9913
#14
Xiu-Lan Chen, Ming Peng, Jing Li, Bai-Lu Tang, Xuan Shao, Fang Zhao, Chang Liu, Xi-Ying Zhang, Ping-Yi Li, Mei Shi, Yu-Zhong Zhang, Xiao-Yan Song
Although several serine collagenolytic proteases from bacteria were reported, none has been used to prepare bioactive collagen peptides. MCP-01 is the most abundant extracellular protease of deep-sea Pseudoalteromonas sp. SM9913 and is a serine collagenolytic protease with high efficiency on fish collagen hydrolysis. Here, we set up a pilot scale process to ferment SM9913 for extracellular protease production. With SM9913 extracellular protease as a tool, a process to prepare collagen oligopeptide-rich hydrolysate from codfish skin was set up, which was further scaled up to pilot (100 L) and plant (2000 L) levels with yields >66%...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146491/respiratory-stress-in-mitochondrial-electron-transport-chain-complex-mutants-of-candida-albicans-activates-snf1-kinase-response
#15
Pengyi Zhang, Hongbin Li, Jie Cheng, April Y Sun, Liqing Wang, Gordana Mircevska, Richard Calderone, Dongmei Li
We have previously established that mitochondrial Complex I (CI) mutants of Candida albicans display reduced oxygen consumption, decreased ATP production, and increased reactive oxidant species (ROS) during cell growth. Using the Seahorse XF96 analyzer, the energetic phenotypes of Electron Transport Chain (ETC) complex mutants are further characterized in the current study. The underlying regulation of energetic changes in these mutants is determined in glucose and non-glucose conditions when compared to wild type (WT) cells...
November 13, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29146359/determination-of-tryptophan-derivatives-in-kynurenine-pathway-in-fermented-foods-using-liquid-chromatography-tandem-mass-spectrometry
#16
Cemile Yılmaz, Vural Gökmen
This study aimed to develop an analytical method for the determination of tryptophan and its derivatives in kynurenine pathway using tandem mass spectrometry in various fermented food products (bread, beer, red wine, white cheese, yoghurt, kefir and cocoa powder). The method entails an aqueous extraction and reversed phase chromatographic separation using pentafluorophenyl (PFP) column. It allowed quantitation of low ppb levels of tryptophan and its derivatives in different fermented food matrices. It was found that beer samples were found to contain kynurenine within the range of 28...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29145855/generation-of-a-platform-strain-for-ionic-liquid-tolerance-using-adaptive-laboratory-evolution
#17
Elsayed T Mohamed, Shizeng Wang, Rebecca M Lennen, Markus J Herrgård, Blake A Simmons, Steven W Singer, Adam M Feist
BACKGROUND: There is a need to replace petroleum-derived with sustainable feedstocks for chemical production. Certain biomass feedstocks can meet this need as abundant, diverse, and renewable resources. Specific ionic liquids (ILs) can play a role in this process as promising candidates for chemical pretreatment and deconstruction of plant-based biomass feedstocks as they efficiently release carbohydrates which can be fermented. However, the most efficient pretreatment ILs are highly toxic to biological systems, such as microbial fermentations, and hinder subsequent bioprocessing of fermentative sugars obtained from IL-treated biomass...
November 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29145125/a-closed-loop-strategy-for-endoglucanase-production-using-sugarcane-bagasse-liquefied-by-a-home-made-enzymatic-cocktail
#18
Paula Squinca, Alberto C Badino, Cristiane S Farinas
Use of the same lignocellulosic biomass as feedstock for enzymes and ethanol production has been suggested as a lower cost option in future biorefineries. Here, we propose a closed-loop strategy to produce the cellulolytic enzymes required for biomass hydrolysis using sugarcane bagasse liquefied by a home-made enzymatic cocktail as carbon source and inducer. The fed-batch liquefaction conditions were firstly evaluated using commercial enzymes. Subsequently, the effects of different liquefied materials and solids loadings on endoglucanase production by Aspergillus niger cultivated in submerged fermentation were investigated...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145123/continuous-liquid-fermentation-of-pretreated-waste-activated-sludge-for-high-rate-volatile-fatty-acids-production-and-online-nutrients-recovery
#19
Lihui Zhang, He Liu, Zhiyong Zheng, Huijun Ma, Meng Yang, Hongbo Liu
Raw sludge was pretreated, and the separated sludge liquid was used as substrate in a continuous operated up-flow anaerobic sludge blanket reactor to produce volatile fatty acids (VFAs). The highest VFA productivity of continuous fermentation with sludge liquid at an organic loading rate (OLR) of 10.0 kg COD/m(3)/d was about 5.0-fold and 4.0-fold higher than batch and semi-continuous fermentation with pretreated sludge slurry, respectively. Moreover, the liquid fermentation with an OLR of 10.0 kg COD/m(3)/d consumed the least energy, which was about 10...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145121/role-of-acid-alkali-treatment-in-primary-sludge-anaerobic-fermentation-insights-into-microbial-community-structure-functional-shifts-and-metabolic-output-by-high-throughput-sequencing
#20
Xiao Huang, Wenyi Dong, Hongjie Wang, Yangyang Feng
This study aimed to investigate the effect of acid- or alkali-treatment on volatile fatty acids (VFAs) production and microbiological mechanism during primary sludge anaerobic fermentation. Seven fermentation experiments were conducted at different pH (3-12). Results showed that the optimal pH was 10 for accumulation of VFAs. High-throughput sequencing results indicated that acid and alkali treatment could inhibit Erysipelotrichaceae_UCG-004 and norank_p_Aminicenantes, instead of promoting Pseudomonas and Tissierella at acidic and alkaline condition, respectively...
November 4, 2017: Bioresource Technology
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