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Ecdysteroids

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https://www.readbyqxmd.com/read/28069934/steroid-signaling-in-mature-follicles-is-important-for-drosophila-ovulation
#1
Elizabeth Knapp, Jianjun Sun
Although ecdysteroid signaling regulates multiple steps in oogenesis, it is not known whether it regulates Drosophila ovulation, a process involving a matrix metalloproteinase-dependent follicle rupture. In this study, we demonstrated that ecdysteroid signaling is operating in mature follicle cells to control ovulation. Moreover, knocking down shade (shd), encoding the monooxygenase that converts ecdysone (E) to the more active 20-hydroxyecdysone (20E), specifically in mature follicle cells, blocked follicle rupture, which was rescued by ectopic expression of shd or exogenous 20E...
January 9, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28069422/identification-of-ecdysteroid-receptor-mediated-signaling-pathways-in-the-hepatopancreas-of-the-red-swamp-crayfish-procambarus-clarkii
#2
Baojian Zhu, Lin Tang, Yingying Yu, Huimin Yu, Lei Wang, Cen Qian, Guoqing Wei, Chaoliang Liu
The hepatopancreas of crustaceans plays an important role in lipid and carbohydrate metabolism, digestion of food, and biogenesis. In this study, the hepatopancreas transcriptome from the red crayfish Procambarus clarkii was characterized for the first time using high-throughput sequencing, producing approximately 41.4 million reads were obtained. After de novo assembly, 57,363 unigenes with an average length of 725bp were identified, Gene Ontology analysis categorized 22,580 as being involved in biological processes, among which metabolic process and cellular process groups were the most highly enriched...
January 6, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27993043/mechanisms-of-action-of-compounds-that-enhance-storage-lipid-accumulation-in-daphnia-magna
#3
Rita Jordão, Bruno Campos, Benjamín Piña, Romà Tauler, Amadeu M V M Soares, Carlos Barata
Accumulation of storage lipids in the crustacean Daphnia magna can be altered by a number of exogenous and endogenous compounds, like 20-hydroxyecdysone (natural ligand of the ecdysone receptor, EcR), methyl farnesoate, pyrirproxyfen (agonists of the methyl farnesoate receptor, MfR), and tributyltin (agonist of the retinoid X acid receptor, RXR). This effect, analogous to the obesogenic disruption in mammals, alters Daphnia's growth and reproductive investment. Here we propose that storage lipid accumulation in droplets is regulated in Daphnia by the interaction between the nuclear receptor heterodimer EcR:RXR and MfR...
December 20, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27976533/phytoecdysteroids-as-antifeedants-towards-several-beetles-that-include-polyphagous-and-monophagous-feeding-guilds
#4
Russell Jurenka, Kathryn Russell, Matthew O'Neal
BACKGROUND: Plants are thought to produce ecdysteroids as a means of protection from insect herbivores. Some insects will not feed on plants containing high amounts of phytoecdysteroids and this response could be limited to monophagous and oligophagous insects. The aim of this study was to determine if phytoecdysteroids could inhibit feeding in several species of beetles that range from monophagous to polyphagous. RESULTS: Here we demonstrate that phytoecdysteroids, including 20-hydroxyecdysone, prevents several beetle species from feeding on preferred host plants, including the polyphagous Japanese beetle, Papillia japonica (Scarabaeidae)...
December 15, 2016: Pest Management Science
https://www.readbyqxmd.com/read/27929032/ecdysteroid-containing-food-supplements-from-cyanotis-arachnoidea-on-the-european-market-evidence-for-spinach-product-counterfeiting
#5
Attila Hunyadi, Ibolya Herke, Katalin Lengyel, Mária Báthori, Zoltán Kele, András Simon, Gábor Tóth, Kálmán Szendrei
Phytoecdysteroids like 20-hydroxyecdysone ("ecdysterone") can exert a mild, non-hormonal anabolic/adaptogenic activity in mammals, and as such, are frequently used in food supplements. Spinach is well-known for its relatively low ecdysteroid content. Cyanotis arachnoidea, a plant native in China, is among the richest sources of phytoecdysteroids, and extracts of this plant are marketed in tons per year amounts via the internet at highly competitive prices. Here we report the investigation of a series of food supplements produced in Germany and claimed to contain spinach extracts...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27907116/rnai-mediated-functional-analysis-of-bursicon-genes-related-to-adult-cuticle-formation-and-tanning-in-the-honeybee-apis-mellifera
#6
Claudinéia Pereira Costa, Moysés Elias-Neto, Tiago Falcon, Rodrigo Pires Dallacqua, Juliana Ramos Martins, Marcia Maria Gentile Bitondi
Bursicon is a heterodimeric neurohormone that acts through a G protein-coupled receptor named rickets (rk), thus inducing an increase in cAMP and the activation of tyrosine hydroxylase, the rate-limiting enzyme in the cuticular tanning pathway. In insects, the role of bursicon in the post-ecdysial tanning of the adult cuticle and wing expansion is well characterized. Here we investigated the roles of the genes encoding the bursicon subunits during the adult cuticle development in the honeybee, Apis mellifera...
2016: PloS One
https://www.readbyqxmd.com/read/27890717/ecdysone-receptor-ecr-and-ultraspiracle-usp-genes-from-the-cyclopoid-copepod-paracyclopina-nana-identification-and-expression-in-response-to-water-accommodated-fractions-wafs
#7
Jayesh Puthumana, Min-Chul Lee, Jeonghoon Han, Hui-Su Kim, Dae-Sik Hwang, Jae-Seong Lee
Ecdysteroid hormones are pivotal in the development, growth, and molting of arthropods, and the hormone pathway is triggered by binding ecdysteroid to a heterodimer of the two nuclear receptors; ecdysone receptors (EcR) and ultraspiracle (USP). We have characterized EcR and USP genes, and their 5'-untranslated region (5'-UTR) from the copepod Paracyclopina nana, and studied mRNA transcription levels in post-embryonic stages and in response to water accommodated fractions (WAFs) of crude oil. The open reading frames (ORF) of EcR and USP were 1470 and 1287bp that encoded 490 and 429 amino acids with molecular weight of 121...
February 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/27815474/effect-of-20-hydroxyecdysone-on-proteolytic-regulation-in-skeletal-muscle-atrophy
#8
Muthita Hirunsai, Tossaporn Yimlamai, Apichart Suksamrarn
BACKGROUND/AIM: 20-Hydroxyecdystone (20E) is an ecdysteroid hormone which controls molting and reproduction in arthropods. 20E also produces a variety of effects in vertebrates, including enhancing protein synthesis and skeletal muscle regeneration. The effect of 20E on disuse muscle atrophy has not been reported to date. This study examined the proteolytic regulation of 20E in tenotomized rat slow soleus and fast plantaris muscles. MATERIALS AND METHODS: Male Wistar rats were randomly divided into three groups: sedentary control (CON), tenotomy without 20E treatment (TEN), and tenotomy with treatment of 5 mg/kg BW of 20E (TEN+20E)...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27810561/bbb-penetration-targeting-physicochemical-lead-selection-ecdysteroids-as-chemo-sensitizers-against-cns-tumors
#9
Judit Müller, Ana Martins, József Csábi, Ferenc Fenyvesi, Árpád Könczöl, Attila Hunyadi, György T Balogh
The anticancer potential of ecdysteroids, especially their chemo-sensitizing activity has recently gained a substantial scientific interest. A comprehensive physicochemical profiling was performed for a set of natural or semi-synthetic ecdysteroids (N=37) to identify a lead compound against central nervous system (CNS) tumors. Calculated properties, such as lipophilicity (clogP), topological polar surface area (TPSA), brain-to-plasma ratio (clogBB) along with the measured blood-brain barrier specific in vitro permeability (logPe) were evaluated in parallel...
January 1, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27788344/synergism-and-antagonism-of-proximate-mechanisms-enable-and-constrain-the-response-to-simultaneous-selection-on-body-size-and-development-time-an-empirical-test-using-experimental-evolution
#10
Goggy Davidowitz, Derek Roff, H Frederik Nijhout
Natural selection acts on multiple traits simultaneously. How mechanisms underlying such traits enable or constrain their response to simultaneous selection is poorly understood. We show how antagonism and synergism among three traits at the developmental level enable or constrain evolutionary change in response to simultaneous selection on two focal traits at the phenotypic level. After 10 generations of 25% simultaneous directional selection on all four combinations of body size and development time in Manduca sexta (Sphingidae), the changes in the three developmental traits predict 93% of the response of development time and 100% of the response of body size...
November 2016: American Naturalist
https://www.readbyqxmd.com/read/27662806/characterization-of-candidate-intermediates-in-the-black-box-of-the-ecdysone-biosynthetic-pathway-in-drosophila-melanogaster-evaluation-of-molting-activities-on-ecdysteroid-defective-larvae
#11
Junki Saito, Ryota Kimura, Yuya Kaieda, Ritsuo Nishida, Hajime Ono
Early steps of the biosynthetic pathway of the insect steroid hormone ecdysone remains the "Black Box" wherein the characteristic ecdysteroid skeleton is built. 7-Dehydrocholesterol (7dC) is the precursor of uncharacterized intermediates in the Black Box. The oxidation step at C-3 has been hypothesized during conversion from 7dC to 3-oxo-2,22,25-trideoxyecdysone, yet 3-dehydroecdysone is undetectable in some insect species. Therefore, we first confirmed that the oxidation at C-3 occurs in the fruitfly, Drosophila melanogaster using deuterium-labeled cholesterol...
October 2016: Journal of Insect Physiology
https://www.readbyqxmd.com/read/27576083/combinatory-annotation-of-cell-membrane-receptors-and-signalling-pathways-of-bombyx-mori-prothoracic-glands
#12
COMMENT
Panagiotis Moulos, Martina Samiotaki, George Panayotou, Skarlatos G Dedos
The cells of prothoracic glands (PG) are the main site of synthesis and secretion of ecdysteroids, the biochemical products of cholesterol conversion to steroids that shape the morphogenic development of insects. Despite the availability of genome sequences from several insect species and the extensive knowledge of certain signalling pathways that underpin ecdysteroidogenesis, the spectrum of signalling molecules and ecdysteroidogenic cascades is still not fully comprehensive. To fill this gap and obtain the complete list of cell membrane receptors expressed in PG cells, we used combinatory bioinformatic, proteomic and transcriptomic analysis and quantitative PCR to annotate and determine the expression profiles of genes identified as putative cell membrane receptors of the model insect species, Bombyx mori, and subsequently enrich the repertoire of signalling pathways that are present in its PG cells...
2016: Scientific Data
https://www.readbyqxmd.com/read/27545316/identification-and-expression-patterns-of-halloween-genes-encoding-cytochrome-p450s-involved-in-ecdysteroid-biosynthesis-in-the-cotton-bollworm-helicoverpa-armigera
#13
J Zheng, K Tian, Y Yuan, M Li, X Qiu
20-Hydroxyecdysone (20E) is a key hormone which regulates growth, development and reproduction in insects. Although cytochrome P450 enzymes (P450s) participating in the ecdysteroid biosynthesis of 20E have been characterized in a few model insects, no work has been published on the molecular entity of their orthologs in the cotton bollworm Helicoverpa armigera, a major pest insect in agriculture worldwide. In this study, four cytochrome P450 homologs, namely HarmCYP302A1, HarmCYP306A1, HarmCYP314A1 and HarmCYP315A1 from H...
August 22, 2016: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/27517937/anti-dengue-virus-constituents-from-formosan-zoanthid-palythoa-mutuki
#14
Jin-Ching Lee, Fang-Rong Chang, Shu-Rong Chen, Yu-Hsuan Wu, Hao-Chun Hu, Yang-Chang Wu, Anders Backlund, Yuan-Bin Cheng
A new marine ecdysteroid with an α-hydroxy group attaching at C-4 instead of attaching at C-2 and C-3, named palythone A (1), together with eight known compounds (2-9) were obtained from the ethanolic extract of the Formosan zoanthid Palythoa mutuki. The structures of those compounds were mainly determined by NMR spectroscopic data analyses. The absolute configuration of 1 was further confirmed by comparing experimental and calculated circular dichroism (CD) spectra. Anti-dengue virus 2 activity and cytotoxicity of five isolated compounds were evaluated using virus infectious system and [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assays, respectively...
August 9, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27497706/mitochondria-and-the-insect-steroid-hormone-receptor-ecr-a-complex-relationship
#15
Xanthe Vafopoulou, Colin G H Steel
The actions of the insect steroid molting hormones, ecdysteroids, on the genome of target cells has been well studied, but little is known of their extranuclear actions. We previously showed in Rhodnius prolixus that much of the ecdysteroid receptor (EcR) resides in the cytoplasm of various cell types and undergoes shuttling between nucleus and cytoplasm with circadian periodicity, possibly using microtubules as tracks for translocation to the nucleus. Here we report that cytoplasmic EcR appears to be also involved in extranuclear actions of ecdysteroids by association with the mitochondria...
October 1, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27494275/drosophila-larval-to-pupal-switch-under-nutrient-stress-requires-ip3r-ca-2-signalling-in-glutamatergic-interneurons
#16
Siddharth Jayakumar, Shlesha Richhariya, O Venkateswara Reddy, Michael J Texada, Gaiti Hasan
Neuronal circuits are known to integrate nutritional information, but the identity of the circuit components is not completely understood. Amino acids are a class of nutrients that are vital for the growth and function of an organism. Here, we report a neuronal circuit that allows Drosophila larvae to overcome amino acid deprivation and pupariate. We find that nutrient stress is sensed by the class IV multidendritic cholinergic neurons. Through live calcium imaging experiments, we show that these cholinergic stimuli are conveyed to glutamatergic neurons in the ventral ganglion through mAChR...
August 5, 2016: ELife
https://www.readbyqxmd.com/read/27470394/hemolin-triggers-cell-survival-on-fibroblasts-in-response-to-serum-deprivation-by-inhibition-of-apoptosis
#17
Rosemary Viola Bosch, Miryam Paola Alvarez-Flores, Durvanei Augusto Maria, Ana Marisa Chudzinski-Tavassi
Fibroblasts are the main cellular component of connective tissues and play important roles in health and disease through the production of collagen, fibronectin and growth factors. Under certain conditions, such as wound healing, fibroblasts intensify their metabolic demand, while the restriction of nutrients affect matrix composition, cell metabolism and behavior. In lepidopterans, wound healing is regulated by ecdysteroid hormones, which upregulate multifunctional proteins such as hemolin. However, the role of hemolin in cell proliferation and wound healing is not clear...
August 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27461053/a-role-of-suppressor-of-cytokine-signaling-2-in-the-regulation-of-ecdysteroid-signaling-pathway-in-procambarus-clarkii
#18
Baojian Zhu, Lishang Dai, Yingying Yu, Daojun Wang, Tao Peng, Chaoliang Liu
A suppressor of cytokine signaling 2 (SOCS-2) homolog was identified from the red crayfish Procambarus clarkii. Phylogenic analysis showed that P. clarkii SOCS-2 (Pc-SOCS-2) was very similar to that of invertebrates. The sequence contains a central SH2 domain, a conserved SOCS box, and a variable N-terminal domain. Real-time polymerase chain reaction showed that Pc-SOCS-2 mRNA level was higher in muscle and heart than in the other tissues examined. A 40-kDa recombinant protein was successfully expressed and purified from Escherichia coli cells as confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting...
August 2016: Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology
https://www.readbyqxmd.com/read/27425007/new-ecdysteroid-and-ecdysteroid-glycosides-from-the-roots-of-serratula-chinensis
#19
Zi-Yue Zhang, Wei-Qun Yang, Chun-Lin Fan, Hui-Nan Zhao, Xiao-Jun Huang, Ying Wang, Wen-Cai Ye
Three new ecdysteroid glycosides (1-3) and one new ecdysteroid (4), were isolated from the roots of Serratula chinensis. Their structures were established on the basis of extensive spectroscopic analysis and chemical methods.
July 18, 2016: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/27424162/physiological-and-molecular-mechanisms-underlying-photoperiodism-in-the-spider-mite-comparisons-with-insects
#20
Shin G Goto
Photoperiodism is an adaptive, seasonal timing system that enables organisms to coordinate their development and physiology to annual changes in the environment using day length (photoperiod) as a cue. This review summarizes our knowledge of the physiological mechanisms underlying photoperiodism in spider mites. In particular, the two-spotted spider mite Tetranychus urticae is focussed, which has long been used as a model species for studying photoperiodism. Photoperiodism is established by several physiological modules, such as the photoreceptor, photoperiodic time measurement system, counter system, and endocrine effector...
July 16, 2016: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
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