Liangguo Xie, Chaoqun Li, Chao Wang, Zhen Wu, Changchun Wang, Chunyu Chen, Xiaojian Chen, Dejian Zhou, Qiang Zhou, Ping Lu, Chen Ding, Chen-Ying Liu, Jinzhong Lin, Xumin Zhang, Xiaofei Yu, Wei Yu
Aspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+ -dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activity by impairing the substrates binding affinity...
March 14, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)