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A I Sánchez-Soto, G I Saavedra-González, J E Ibarra, R Salcedo-Hernández, J E Barboza-Corona, M C Del Rincón-Castro
UNLABELLED: The insecticidal activity of Bacillus thuringiensis is owing to the action of Cry and Cyt proteins. In addition to the synthesis of insecticidal proteins, some strains are able to synthesize β-exotoxin, which is highly toxic to humans. In this regard, it is very important to have a simple method to detect β-exotoxin to avoid the commercial production of this type of strains. In this work, we developed a simple and fast method, using the nematode Caenorhabditis elegans to detect indirectly the synthesis of β-exotoxin by B...
December 2015: Letters in Applied Microbiology
José López-López, Enric Jané-Salas, Amparo Santamaría, Beatriz González-Navarro, Carlos Arranz-Obispo, Ramón López, Ignasi Miquel, Belén Arias, Pilar Sánchez, Esther Rincón, Juan R Rodríguez, Santiago Rojas, Jesus Murat
OBJECTIVES: TT-173 is a new hemostatic agent consisting of yeast-derived microvesicles containing a modified version of recombinant human tissue factor. In the present work, the procoagulant activity of TT-173 has been evaluated for the first time in humans. METHODS: This is a phase I, randomized, placebo-controlled study to evaluate the efficacy, safety, systemic absorption, and immunogenicity of TT-173 in healthy volunteers undergoing tooth extraction. Subjects received TT-173 or placebo into the alveolar cavity, just after tooth extraction...
June 2016: Clinical Oral Investigations
Cristina Pérez, Julia González-Rincón, Arantza Onaindia, Carmen Almaráz, Nuria García-Díaz, Helena Pisonero, Soraya Curiel-Olmo, Sagrario Gómez, Laura Cereceda, Rebeca Madureira, Mercedes Hospital, Dolores Suárez-Massa, José L Rodriguez-Peralto, Concepción Postigo, Alicia Leon-Castillo, Carmen González-Vela, Nerea Martinez, Pablo Ortiz-Romero, Margarita Sánchez-Beato, Miguel Á Piris, José P Vaqué
No abstract text is available yet for this article.
November 2015: Haematologica
Paula González-Alonso, Cristina Chamizo, Víctor Moreno, Juan Madoz-Gúrpide, Nerea Carvajal, Lina Daoud, Sandra Zazo, Ester Martín-Aparicio, Ion Cristóbal, Raúl Rincón, Jesús García-Foncillas, Federico Rojo
Mutations in Human Epidermal Growth Factor Receptors (HER) are associated with poor prognosis of several types of solid tumors. Although HER-mutation detection methods are currently available, such as Next-Generation Sequencing (NGS), alternative pyrosequencing allow the rapid characterization of specific mutations. We developed specific PCR-based pyrosequencing assays for identification of most prevalent HER2 and HER3 mutations, including S310F/Y, R678Q, L755M/P/S/W, V777A/L/M, 774-776 insertion, and V842I mutations in HER2, as well as M91I, V104M/L, D297N/V/Y, and E332E/K mutations in HER3...
2015: International Journal of Molecular Sciences
David Morales-Alamo, José Losa-Reyna, Rafael Torres-Peralta, Marcos Martin-Rincon, Mario Perez-Valera, David Curtelin, Jesús Gustavo Ponce-González, Alfredo Santana, José A L Calbet
To determine the mechanisms causing task failure during incremental exercise to exhaustion (IE), sprint performance (10 s all-out isokinetic) and muscle metabolites were measured before (control) and immediately after IE in normoxia (P(IO2) 143 mmHg) and hypoxia (P(IO2): 73 mmHg) in 22 men (22 ± 3 years). After IE, subjects recovered for either 10 or 60 s, with open circulation or bilateral leg occlusion (300 mmHg) in random order. This was followed by a 10 s sprint with open circulation. Post-IE peak power output (W(peak)) was higher than the power output reached at exhaustion during IE (P < 0...
October 15, 2015: Journal of Physiology
Gabriela M Montero-Morán, José G Sampedro, Gloria Saab-Rincón, Miguel A Cervantes-González, José Á Huerta-Ocampo, Antonio De León-Rodríguez, Ana P Barba de la Rosa
A novel Cu/ZnSOD from Amaranthus hypochondriacus was cloned, expressed, and characterized. Nucleotide sequence analysis showed an open reading frame (ORF) of 456 bp, which was predicted to encode a 15.6-kDa molecular weight protein with a pI of 5.4. Structural analysis showed highly conserved amino acid residues involved in Cu/Zn binding. Recombinant amaranth superoxide dismutase (rAhSOD) displayed more than 50 % of catalytic activity after incubation at 100 °C for 30 min. In silico analysis of Amaranthus hypochondriacus SOD (AhSOD) amino acid sequence for globularity and disorder suggested that this protein is mainly disordered; this was confirmed by circular dichroism, which showed the lack of secondary structure...
August 2015: Applied Biochemistry and Biotechnology
Jose María González-Santos, María Elena Arnáiz-García, Jose Alfonso Sastre-Rincón, María E Bueno-Codoñer, María José Dalmau-Sorlí, Adolfo Arévalo-Abascal, Javier López-Rodríguez, Alejandro Diego-Nieto
A patient was submitted to mitral valve replacement and tricuspid ring annuloplasty. During immediate postoperative course, signs of inferior myocardial ischemia appeared. Acute entrapment of the right coronary artery due to tricuspid ring sutures was confirmed by coronary angiography. The patient was reoperated and a right coronary bypass graft was successfully performed. Tricuspid procedures have shown to be effective and secure with a low rate of complication. Few cases of right coronary artery occlusion have been described and the majority not treated...
June 2015: Annals of Thoracic Surgery
D Pérez-Callejo, J González-Rincón, A Sánchez, M Provencio, M Sánchez-Beato
Anti-CD20 monoclonal antibodies (mAbs) have improved patient's survival with Non-Hodgkin Lymphoma, when combined with chemotherapy. Several mechanisms of action have been reported, including antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity and induction of apoptosis. Despite the large amount of studies and published data, the role each mechanism played in vivo is not fully understood. Furthermore, the reason why a significant percentage of patients are refractory or resistant remains unknown...
September 2015: Cancer Treatment Reviews
Sabela Lens, Diego Rincón, Montserrat García-Retortillo, Agustín Albillos, Jose Luis Calleja, Rafael Bañares, Juan González Abraldes, Jaume Bosch, Jose Maria Sanchez-Tapias, Xavier Forns, Juan Carlos García-Pagán
BACKGROUND & AIMS: Hepatic venous pressure gradient (HVPG) is associated with a risk of liver events in patients with chronic hepatitis C. Antiviral therapies that lead to a sustained virologic response (SVR) reduce portal pressure and prevent liver disease progression. However, it is not clear to what extent the progression of hepatitis C is modified once patients develop cirrhosis with severe portal hypertension (CSPH) (HVPG ≥ 10 mm Hg). We assessed the effects of HVPG and SVR on the risk of liver decompensation, hepatocellular carcinoma, and/or death in patients with hepatitis C-related cirrhosis...
October 2015: Clinical Gastroenterology and Hepatology
Javier García-Lledó, Ana Clemente-Sánchez, Beatriz Merino-Rodríguez, Óscar Nogales-Rincón, Cecilia González-Asanza, Pedro Menchén-Fernández-Pacheco
No abstract text is available yet for this article.
April 2015: Revista Española de Enfermedades Digestivas
Óscar Aguilera, José Manuel González-Sancho, Sandra Zazo, Raúl Rincón, Agustín F Fernández, Olga Tapia, Francesc Canals, Beatriz Morte, Vincenzo Calvanese, José L Orgaz, Núria Niell, Susana Aguilar, José M Freije, Osvaldo Graña, David G Pisano, Aurea Borrero, Javier Martínez-Useros, Benilde Jiménez, Mario F Fraga, Jesús García-Foncillas, Carlos López-Otín, Miguel Lafarga, Federico Rojo, Alberto Muñoz
Sporadic colorectal cancer (CRC) insurgence and progression depend on the activation of Wnt/β-catenin signaling. Dickkopf (DKK)-1 is an extracellular inhibitor of Wnt/β-catenin signaling that also has undefined β-catenin-independent actions. Here we report for the first time that a proportion of DKK-1 locates within the nucleus of healthy small intestine and colon mucosa, and of CRC cells at specific chromatin sites of active transcription. Moreover, we show that DKK-1 regulates several cancer-related genes including the cancer stem cell marker aldehyde dehydrogenase 1A1 (ALDH1A1) and Ral-binding protein 1-associated Eps domain-containing 2 (REPS2), which are involved in detoxification of chemotherapeutic agents...
March 20, 2015: Oncotarget
Robinson Ramírez-Vélez, Katherine González-Ruíz, Jorge Correa-Bautista, Javier Martínez-Torres, José F Meneses-Echávez, David Rincon-Pabon
OBJECTIVE: Ferritin deficiency is associated with many adverse health outcomes and is highly prevalent worldwide. The present study assesses the prevalence and socio-demographic factors associated with ferritin deficiency in a representative sample of pregnant women in Colombia. METHODS: We used data from the cross-sectional, nationally representative survey National Nutritional Survey (ENSIN, 2010). A total of 1,386, (13-49 years old) pregnant women were enrolled...
2015: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
J C Garzón Sánchez, V J Santana González, T López Correa, J A Sastre Rincón
No abstract text is available yet for this article.
November 2015: Revista Española de Anestesiología y Reanimación
Aarón Emmanuel González-Esquivel, Claudia Lisset Charles-Niño, Fermín Paul Pacheco-Moisés, Genaro Gabriel Ortiz, Fernando Jaramillo-Juárez, Ana Rosa Rincón-Sánchez
TiO2 nanoparticles used as vectors for the delivery of drugs have shown greater effectiveness. However, TiO2 nanoparticles can cause oxidative stress in liver and kidney, so we analyzed if a previous or simultaneous quercetin treatment could counteract this in rats. Five groups of male Wistar rats (200-250 g) were included: (1) healthy controls, (2) TiO2 group, (3) quercetin group, (4) preventive group: quercetin for 5 days prior to exposure of TiO2, and (5) therapeutic group: TiO2 (5 mg/kg, i.v.) plus quercetin single dose for 5 days (5 mg/kg/day, i...
March 2015: Toxicology Mechanisms and Methods
William A Mehan, R Gilberto González, Bradley R Buchbinder, John W Chen, William A Copen, Rajiv Gupta, Joshua A Hirsch, George J Hunter, Scott Hunter, Jason M Johnson, Hillary R Kelly, Mykol Larvie, Michael H Lev, Stuart R Pomerantz, Otto Rapalino, Sandra Rincon, Javier M Romero, Pamela W Schaefer, Vinil Shah
BACKGROUND/PURPOSE: Patients with neurologic complaints are imaged with MRI protocols that may include many pulse sequences. It has not been documented which sequences are essential. We assessed the diagnostic accuracy of a limited number of sequences in patients with new neurologic complaints. METHODS: 996 consecutive brain MRI studies from patients with new neurological complaints were divided into 2 groups. In group 1, reviewers used a 3-sequence set that included sagittal T1-weighted, axial T2-weighted fluid-attenuated inversion recovery, and axial diffusion-weighted images...
2014: PloS One
Irma Gonzalez-Curiel, Valentin Trujillo, Alejandra Montoya-Rosales, Kublai Rincon, Bruno Rivas-Calderon, Jeny deHaro-Acosta, Paulina Marin-Luevano, Daniel Lozano-Lopez, Jose A Enciso-Moreno, Bruno Rivas-Santiago
Diabetic foot ulcers (DFU) are one of the most common diabetes-related cause of hospitalization and often lead to severe infections and poor healing. It has been recently reported that patients with DFU have lower levels of antimicrobial peptides (AMPs) at the lesion area, which contributes with the impairment of wound healing. The aim of this study was to determine whether 1,25-dihydroxyvitamin D3 (1,25 (OH)2 D3) and L-isoleucine induced HBD-2 and LL-37 in primary cultures from DFU. We developed primary cell cultures from skin biopsies from 15 patients with DFU and 15 from healthy donors...
2014: PloS One
Alejandra Ochoa-Palominos, Fernando Díaz-Fontenla, Cecilia González-Asanza, Beatriz Merino-Rodríguez, Óscar Nogales-Rincón, Pedro Menchén-Fernández-Pacheco
No abstract text is available yet for this article.
May 2014: Revista Española de Enfermedades Digestivas
María Elena Arnáiz-García, Jose María González-Santos, María E Bueno-Codoñer, Jose Alfonso Sastre Rincón, Alberto Iscar-Galán
No abstract text is available yet for this article.
2014: Kardiologia Polska
Luisa Fernanda Duarte, Oscar Flórez, Giovanna Rincón, Clara Isabel González
OBJECTIVE: To compare the diagnostic performance of seven methods to determine Trypanosoma cruzi infection in patients with chronic Chagas disease. METHODS: Analytical study, using the case-control design, which included 205 people (patients with Chagasic cardiomyopathy, n=100; control group, n=105). Three enzyme linked immunosorbent assays, one indirect hemagglutination assay and one immunochromatographic test were assessed. Additionally, DNA amplification was performed via the PCR method using kinetoplast and nuclear DNA as target sequences...
April 2014: Colombia Médica: CM
Maria Luz González Fernández, Rosario Morales Lozano, Carmen Martínez Rincón, David Martínez Hernández
BACKGROUND: We sought to assess the biomechanical characteristics of the feet of patients with Charcot neuro-osteoarthropathy and to determine reulceration rates before and after personalized conservative orthotic treatment. METHODS: A longitudinal prospective study was performed in 35 patients with Charcot's foot. Although some patients had a history of ulcers, at the study outset no patient had ulcers. All of the patients underwent biomechanical testing and a radiographic study...
July 2014: Journal of the American Podiatric Medical Association
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