keyword
https://read.qxmd.com/read/34361079/liver-specific-overexpression-of-prostasin-attenuates-high-fat-diet-induced-metabolic-dysregulation-in-mice
#21
JOURNAL ARTICLE
Tetsuo Sekine, Soichi Takizawa, Kohei Uchimura, Asako Miyazaki, Kyoichiro Tsuchiya
The liver has a most indispensable role in glucose and lipid metabolism where we see some of the most serious worldwide health problems. The serine protease prostasin (PRSS8) cleaves toll-like receptor 4 (TLR4) and regulates hepatic insulin sensitivity under PRSS8 knockout condition. However, liver substrate proteins of PRSS8 other than TLR4 and the effect to glucose and lipid metabolism remain unclarified with hepatic elevation of PRSS8 expression. Here we show that high-fat-diet-fed liver-specific PRSS8 transgenic mice improved glucose tolerance and hepatic steatosis independent of body weight...
August 2, 2021: International Journal of Molecular Sciences
https://read.qxmd.com/read/34347114/gene-expression-microarray-analysis-of-adult-testicular-germ-cell-tumor-a-comparison-between-pure-type-seminomas-and-seminoma-components-in-mixed-tumors
#22
COMPARATIVE STUDY
Kosuke Miyai, Yuiko Yonekura, Keiichi Ito, Susumu Matsukuma, Hitoshi Tsuda
We previously demonstrated a genetic evidence of the progression from seminoma to embryonal carcinoma in mixed testicular germ cell tumors (TGCTs). This process, the "reprogramming" of seminoma cells, is crucial for pathological tumorigenesis and should be kept in mind while designing clinical therapeutic strategies. We hypothesized that a comparison between pure-type seminomas and seminoma components in mixed tumors (mixed-type seminomas) could reveal early changes in the reprogramming process. In the present study, we performed gene expression microarray analysis of six pure-type and six mixed-type seminomas...
December 2021: Virchows Archiv: An International Journal of Pathology
https://read.qxmd.com/read/34276354/computational-drug-repurposing-for-alzheimer-s-disease-using-risk-genes-from-gwas-and-single-cell-rna-sequencing-studies
#23
JOURNAL ARTICLE
Yun Xu, Jiming Kong, Pingzhao Hu
Background: Traditional therapeutics targeting Alzheimer's disease (AD)-related subpathologies have so far proved ineffective. Drug repurposing, a more effective strategy that aims to find new indications for existing drugs against other diseases, offers benefits in AD drug development. In this study, we aim to identify potential anti-AD agents through enrichment analysis of drug-induced transcriptional profiles of pathways based on AD-associated risk genes identified from genome-wide association analyses (GWAS) and single-cell transcriptomic studies...
2021: Frontiers in Pharmacology
https://read.qxmd.com/read/34240739/prostasin-regulates-pd-l1-expression-in-human-lung-cancer-cells
#24
JOURNAL ARTICLE
Li-Mei Chen, Julius C Chai, Bin Liu, Tara M Strutt, K Kai McKinstry, Karl X Chai
The serine protease prostasin is a negative regulator of lipopolysaccharide-induced inflammation and has a role in the regulation of cellular immunity. Prostasin expression in cancer cells inhibits migration and metastasis, and reduces epithelial-mesenchymal transition. Programmed death-ligand 1 (PD-L1) is a negative regulator of the immune response and its expression in cancer cells interferes with immune surveillance. The aim of the present study was to investigate if prostasin regulates PD-L1 expression...
July 30, 2021: Bioscience Reports
https://read.qxmd.com/read/34195807/prostasin-regulates-pd-l1-expression-in-human-lung-cancer-cells
#25
JOURNAL ARTICLE
Li-Mei Chen, Julius C Chai, Bin Liu, Tara M Strutt, K Kai McKinstry, Karl X Chai
The serine protease prostasin is a negative regulator of lipopolysaccharide-induced inflammation and has a role in the regulation of cellular immunity.  Prostasin expression in cancer cells inhibits migration and metastasis, and reduces epithelial-mesenchymal transition.  Programmed death-ligand 1 (PD-L1) is a negative regulator of the immune response and its expression in cancer cells interferes with immune surveillance.  The aim of this study was to investigate if prostasin regulates PD-L1 expression.  We established sublines over-expressing various forms of prostasin as well as a subline deficient for the prostasin gene from the Calu-3 human lung cancer cells...
June 28, 2021: Bioscience Reports
https://read.qxmd.com/read/33987838/mapping-of-the-amniotic-fluid-proteome-of-fetuses-with-congenital-anomalies-of-the-kidney-and-urinary-tract-identifies-plastin-3-as-a-protein-involved-in-glomerular-integrity
#26
JOURNAL ARTICLE
Camille Fédou, Mylène Camus, Ophélie Lescat, Guylène Feuillet, Ilka Mueller, Bryony Ross, Marie Buléon, Eric Neau, Melinda Alves, Dominique Goudounéche, Benjamin Breuil, Franck Boizard, Quentin Bardou, Audrey Casemayou, Ivan Tack, Sophie Dreux, Julie Batut, Patrick Blader, Odile Burlet-Schiltz, Stéphane Decramer, Brunhilde Wirth, Julie Klein, Jean Sébastien Saulnier-Blache, Bénédicte Buffin-Meyer, Joost P Schanstra
Congenital anomalies of the kidney and the urinary tract (CAKUT) are the first cause of chronic kidney disease in childhood. Several genetic and environmental origins are associated with CAKUT, but most pathogenic pathways remain elusive. Considering the amniotic fluid (AF) composition as a proxy for fetal kidney development, we analyzed the AF proteome from non-severe CAKUT (n=19), severe CAKUT (n=14), and healthy control (n=22) fetuses using LC-MS/MS. We identified 471 significant proteins that discriminated the 3 AF groups with 81% precision...
May 13, 2021: Journal of Pathology
https://read.qxmd.com/read/33650216/zymogen-locked-mutant-prostasin-prss8-leads-to-incomplete-proteolytic-activation-of-the-epithelial-sodium-channel-enac-and-severely-compromises-triamterene-tolerance-in-mice
#27
JOURNAL ARTICLE
Daniel Essigke, Alexandr V Ilyaskin, Matthias Wörn, Bernhard N Bohnert, Mengyun Xiao, Christoph Daniel, Kerstin Amann, Andreas L Birkenfeld, Roman Szabo, Thomas H Bugge, Christoph Korbmacher, Ferruh Artunc
AIM: The serine protease prostasin (Prss8) is expressed in the distal tubule and stimulates proteolytic activation of the epithelial sodium channel (ENaC) in co-expression experiments in vitro. The aim of this study was to explore the role of prostasin in proteolytic ENaC activation in the kidney in vivo. METHODS: We used genetically modified knockin mice carrying a Prss8 mutation abolishing proteolytic activity (Prss8-S238A) or a mutation leading to a zymogen-locked state (Prss8-R44Q)...
May 2021: Acta Physiologica
https://read.qxmd.com/read/33619325/correction-to-prss8-suppresses-colorectal-carcinogenesis-and-metastasis
#28
Yonghua Bao, Yongchen Guo, Yiqiong Yang, Xiaonan Wei, Shanshan Zhang, Yongmeng Zhang, Kai Li, Ming Yuan, Dongli Guo, Virgilia Macias, Xiangdong Zhu, Wei Zhang, Wancai Yang
No abstract text is available yet for this article.
March 2021: Oncogene
https://read.qxmd.com/read/32909001/the-potential-role-of-extracellular-vesicles-in-covid-19-associated-endothelial-injury-and-pro-inflammation
#29
Balaji Krishnamachary, Christine Cook, Leslie Spikes, Prabhakar Chalise, Navneet K Dhillon
COVID-19 infection caused by the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in a global pandemic with the number of deaths growing exponentially. Early evidence points to significant endothelial dysfunction, micro-thromboses, pro-inflammation as well as a dysregulated immune response in the pathogenesis of this disease. In this study, we analyzed the cargo of EVs isolated from the plasma of patients with COVID-19 for the identification of potential biomarkers of disease severity and to explore their role in disease pathogenesis...
September 1, 2020: medRxiv
https://read.qxmd.com/read/32348690/linp1-promotes-the-progression-of-cervical-cancer-by-scaffolding-ezh2-lsd1-and-dnmt1-to-inhibit-the-expression-of-klf2-and-prss8
#30
JOURNAL ARTICLE
Liuli Wu, Yuan Gong, Ting Yan, Huimin Zhang
A growing body of evidence demonstrates that long non-coding RNAs (lncRNAs) are correlated with various cancers. LncRNA LINP1 has been proven as a key factor of malignant tumor development. However, it is still unclear about the role of LINP1 in cervical cancer (CC). In our research, a prominent increase of LINP1 was found in CC tissues in comparison with adjacent normal tissues. What's more, LINP1 expression was identified to be upregulated in CC cells compared with human cervical epithelial cell lines (HUCEC)...
April 29, 2020: Biochemistry and Cell Biology
https://read.qxmd.com/read/31998788/identification-of-key-genes-and-prognostic-analysis-between-chromophobe-renal-cell-carcinoma-and-renal-oncocytoma-by-bioinformatic-analysis
#31
JOURNAL ARTICLE
Hongwei Wu, Lijing Fan, Haiping Liu, Baozhang Guan, Bo Hu, Fanna Liu, Berthold Hocher, Lianghong Yin
The present techniques of clinical and histopathological diagnosis hardly distinguish chromophobe renal cell carcinoma (ChRCC) from renal oncocytoma (RO). To identify differentially expressed genes (DEGs) as effective biomarkers for diagnosis and prognosis of ChRCC and RO, three mRNA microarray datasets (GSE12090, GSE19982, and GSE8271) were downloaded from the GEO database. Functional enrichment analysis of DEGs was performed by DAVID. STRING and Cytoscape were applied to construct the protein-protein interaction (PPI) network and key modules of DEGs...
2020: BioMed Research International
https://read.qxmd.com/read/31916038/sodium-absorption-stimulator-prostasin-prss8-has-an-anti-inflammatory-effect-via-downregulation-of-tlr4-signaling-in-inflammatory-bowel-disease
#32
JOURNAL ARTICLE
Yoshihiko Sugitani, Atsushi Nishida, Osamu Inatomi, Masashi Ohno, Takayuki Imai, Masahiro Kawahara, Kenichiro Kitamura, Akira Andoh
BACKGROUND: Prostasin (PRSS8) is a stimulator of epithelial sodium transport. In this study, we evaluated alteration of prostasin expression in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and investigated the role of prostasin in the gut inflammation. METHODS: Prostasin expression was evaluated by immunohistochemical staining. Dextran sodium sulfate (DSS)-colitis was induced in mice lacking prostasin specifically in intestinal epithelial cells (PRSS8ΔIEC mice)...
April 2020: Journal of Gastroenterology
https://read.qxmd.com/read/31731486/the-role-of-aberrant-dna-methylation-in-misregulation-of-gene-expression-in-gonadotroph-nonfunctioning-pituitary-tumors
#33
JOURNAL ARTICLE
Paulina Kober, Joanna Boresowicz, Natalia Rusetska, Maria Maksymowicz, Agnieszka Paziewska, Michalina Dąbrowska, Jacek Kunicki, Wiesław Bonicki, Jerzy Ostrowski, Janusz A Siedlecki, Mateusz Bujko
Gonadotroph nonfunctioning pituitary adenomas (NFPAs) are common intracranial tumors, but the role of aberrant epigenetic regulation in their development remains poorly understood. In this study, we investigated the effect of impaired CpG methylation in NFPAs. We determined DNA methylation and transcriptomic profiles in 32 NFPAs and normal pituitary sections using methylation arrays and sequencing, respectively. Ten percent of differentially methylated CpGs were correlated with gene expression, and the affected genes are involved in a variety of tumorigenesis-related pathways...
October 25, 2019: Cancers
https://read.qxmd.com/read/31701992/comparison-of-liver-gene-expression-by-rnaseq-and-pcr-analysis-after-8-weeks-of-feeding-soy-protein-isolate-or-casein-based-diets-in-an-obese-liver-steatosis-rat-model
#34
JOURNAL ARTICLE
Melisa Kozaczek, Walter Bottje, Elizabeth Greene, Kentu Lassiter, Byungwhi Kong, Sami Dridi, Soheila Korourian, Reza Hakkak
Previously, we reported that feeding soy protein isolate (SPI) reduced liver steatosis in obese rats compared to those fed a casein (CAS)-based diet; however, the mechanism for this protection is unknown. To gain insight into the ability of SPI to ameliorate liver steatosis, we conducted transcriptomic (RNAseq) analysis on liver samples from obese rats fed either the SPI- or CAS-based diets (n = 8 per group) for 8 weeks using an Ilumina HiSeq with 100 base paired end reads for sequencing. Data were analyzed by Ingenuity Pathway Analysis (IPA) software using a P < 0...
November 8, 2019: Food & Function
https://read.qxmd.com/read/31215738/proteomic-analysis-of-seminal-extracellular-vesicle-proteins-involved-in-asthenozoospermia-by-itraq
#35
JOURNAL ARTICLE
Yu Lin, Ajuan Liang, Yue He, Zhengzheng Li, Zhenhua Li, Guishuan Wang, Fei Sun
Asthenozoospermia is a common cause of male infertility, but in most cases its etiology is unknown. The exocytic cell vesicles called seminal extracellular vesicles in the human seminal fluid have been reported to play a pivotal role in promoting the motility of spermatozoa, and functional disorder of seminal extracellular vesicles may cause male infertility. To determine whether abnormal seminal extracellular vesicles are involved in asthenozoospermia, the differential abundance proteins between normozoospermic (NSEV) and asthenozoospermic seminal extracellular vesicles (ASEV) samples were analyzed by iTRAQ coupled with two-dimensional liquid chromatography-tandem mass spectrometry...
June 19, 2019: Molecular Reproduction and Development
https://read.qxmd.com/read/31081307/unraveling-a-difficult-diagnosis-the-tricks-for-early-recognition-of-ovarian-cancer
#36
JOURNAL ARTICLE
Pierluigi Giampaolino, Luigi Della Corte, Virginia Foreste, Salvatore G Vitale, Benito Chiofalo, Stefano Cianci, Fulvio Zullo, Giuseppe Bifulco
Epithelial ovarian cancer (EOC) is the predominant type of ovarian cancer (OC). The 5-year survival of patients has improved over the last three decades, athough the overall cure rate of OC if about 30%. Despite high response rates after initial chemotherapy, most patients with advanced ovarian cancer ultimately develop the recurrent disease because of resistance to chemotherapy. A proper early diagnosis and treatment of patients with ovarian cancer are urgently needed. Nowadays the diagnosis is performed by means of clinical symptoms and signs, often indicators of a disease already at an advanced stage, tumor markers (CA125 and HE4), transvaginal ultrasonography and imaging, very useful in distinguishing adnexal masses...
May 6, 2019: Minerva Medica
https://read.qxmd.com/read/30875130/expression-of-new-genes-in-vertebrate-tooth-development-and-p63-signaling
#37
JOURNAL ARTICLE
Nasim Rostampour, Cassy M Appelt, Aunum Abid, Julia C Boughner
BACKGROUND: P63 is an evolutionarily ancient transcription factor essential to vertebrate tooth development. Our recent gene expression screen comparing wild-type and "toothless" p63-/- mouse embryos implicated in tooth development several new genes that we hypothesized act downstream of p63 in dental epithelium, where p63 is also expressed. RESULTS: Via in situ hybridization and immunohistochemistry, we probed mouse embryos (E10.5-E14.5) and spotted gar fish embryos (14 days post-fertilization) for these newly linked genes - Cbln1, Cldn23, Fermt1, Krt15, Pltp and Prss8 - which were expressed in mouse and gar dental epithelium...
March 15, 2019: Developmental Dynamics
https://read.qxmd.com/read/30815891/genome-wide-association-study-identifies-markers-associated-with-meat-ultimate-ph-in-duroc-pigs
#38
JOURNAL ARTICLE
R Davoli, M Zappaterra, P Zambonelli
In this study we aimed to identify genomic regions associated with muscle pH, meat colour and water-holding capacity in a population of 280 Italian Duroc pigs genotyped by the Illumina PorcineSNP60 v2 Genotyping BeadChip. After quality control, the remaining 32 597 SNPs and 278 subjects were used to perform a genome-wide association study with the genabel package, using a kinship matrix in a model with the effects of sex, age and slaughter day. Bonferroni correction was applied, and the significant markers and regions were then further investigated to identify the nearest genes and the linkage disequilibrium (LD) between markers...
February 28, 2019: Animal Genetics
https://read.qxmd.com/read/30623107/hepatocyte-growth-factor-activator-inhibitor-2-stabilizes-epcam-and-maintains-epithelial-organization-in-the-mouse-intestine
#39
JOURNAL ARTICLE
Makiko Kawaguchi, Koji Yamamoto, Naoki Takeda, Tsuyoshi Fukushima, Fumiki Yamashita, Katsuaki Sato, Kenichiro Kitamura, Yoshitaka Hippo, James W Janetka, Hiroaki Kataoka
Mutations in SPINT2 encoding the epithelial serine protease inhibitor hepatocyte growth factor activator inhibitor-2 (HAI-2) are associated with congenital tufting enteropathy. However, the functions of HAI-2 in vivo are poorly understood. Here we used tamoxifen-induced Cre-LoxP recombination in mice to ablate Spint2 . Mice lacking Spint2 died within 6 days after initiating tamoxifen treatment and showed severe epithelial damage in the whole intestinal tracts, and, to a lesser extent, the extrahepatic bile duct...
2019: Communications Biology
https://read.qxmd.com/read/30115975/prss8-suppresses-colorectal-carcinogenesis-and-metastasis
#40
JOURNAL ARTICLE
Yonghua Bao, Yongchen Guo, Yiqiong Yang, Xiaonan Wei, Shanshan Zhang, Yongmeng Zhang, Kai Li, Ming Yuan, Dongli Guo, Virgilia Macias, Xiangdong Zhu, Wei Zhang, Wancai Yang
The serine protease PRSS8 has shown important physiological and pathological functions, but its roles in cancer initiation and progression are unclear. We developed and dynamically characterized a conditional knockout Prss8fl/fl , p-Villin-Cre+ mouse model. We found that genetic deficiency of the Prss8 gene caused spontaneous colitis and an inflamed rectum at an early age and caused intestinal tumors at a late age, which were linked to increased intestinal cell proliferation and migration but decreased cell differentiation...
January 2019: Oncogene
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