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https://www.readbyqxmd.com/read/28735505/role-of-infarct-location-and-pre-existing-depression-on-cardiac-baroreceptor-sensitivity-in-subacute-ischemic-stroke
#1
Aurelie De Vos, Jacques De Keyser, Sylvie De Raedt
Reduced cardiac baroreceptor sensitivity (BRS) after acute stroke is associated with worse outcome. The underlying mechanisms of reduced BRS are unclear. We evaluated cross correlation BRS (xBRS) in 184 patients with suspected acute ischemic stroke within 72 h of symptom onset. Among these patients, 22 had a transient ischemic attack (TIA) and 27 had a stroke mimic. Sixty-four age- and sex-matched ambulant control subjects without stroke were included. Compared with controls, xBRS was significantly lower in patients with ischemic stroke, TIA, and stroke mimics (4...
July 22, 2017: Acta Neurologica Belgica
https://www.readbyqxmd.com/read/28735491/nanofluidic-allele-specific-digital-pcr-method-for-quantifying-idh1-and-idh2-mutation-burden-in-acute-myeloid-leukemia
#2
Daniel H Wiseman, Tim C P Somervaille
Precise quantitation of allelic burden for a pathogenic mutation has diverse clinical and research applications but can be difficult to achieve with conventional qPCR-based techniques, especially at lower mutant allele frequencies. Digital PCR overcomes many of the limitations of qPCR and can be highly quantitative even for single-nucleotide variants, with distinct advantages over next-generation sequencing approaches. Here we describe a method combining the principles of TaqMan(®)-chemistry SNP genotyping with microfluidic digital PCR to generate a highly sensitive, quantitative allele-specific digital PCR assay for the six most common IDH1 and IDH2 mutations encountered in myeloid malignancy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735463/impact-of-pioglitazone-on-bone-mineral-density-and-bone-marrow-fat-content
#3
L M Pop, I Lingvay, Q Yuan, X Li, B Adams-Huet, N M Maalouf
Pioglitazone use is associated with an increased risk of fractures. In this randomized, placebo-controlled study, pioglitazone use for 12 months was associated with a significant increase in bone marrow fat content at the femoral neck, accompanied by a significant decrease in total hip bone mineral density. The change in bone marrow fat with pioglitazone use was predominantly observed in female vs. male participants. INTRODUCTION: Use of the insulin sensitizer pioglitazone is associated with greater fracture incidence, although the underlying mechanisms are incompletely understood...
July 22, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28735457/impact-of-sentinel-lymph-node-biopsy-by-ultrasound-guided-core-needle-biopsy-for-patients-with-suspicious-node-positive-breast-cancer
#4
Rikiya Nakamura, Naohito Yamamoto, Toshiko Miyaki, Makiko Itami, Nobumitsu Shina, Masayuki Ohtsuka
PURPOSE: The purpose of this study was to investigate the accuracy of preoperative diagnostic tools for axillary lymph nodes (LNs) staging of breast cancer. MATERIALS AND METHODS: A total of 2464 consecutive patients with operable breast cancer were prospectively identified at our institution between April 2012 and March 2017. Patients with suspicious axillary LN of breast cancer were assessed using preoperative ultrasound(US) or computed tomography (CT), underwent fine-needle aspiration cytology (FNA) or core needle biopsy (CNB)...
July 22, 2017: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/28735452/characterization-of-polymeric-substance-classes-in-cereal-based-beverages-using-asymmetrical-flow-field-flow-fractionation-with-a-multi-detection-system
#5
Georg Krebs, Thomas Becker, Martina Gastl
Cereal-based beverages contain a complex mixture of various polymeric macromolecules including polysaccharides, peptides, and polyphenols. The molar mass of polymers and their degradation products affect different technological and especially sensory parameters of beverages. Asymmetrical flow field-flow fractionation (AF4) coupled with multi-angle light scattering (MALS) and refractive index detection (dRI) or UV detection (UV) is a technique for structure and molar mass distribution analysis of macromolecules commonly used for pure compound solutions...
July 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28735447/cost-benefit-analysis-of-two-child-abuse-and-neglect-primary-prevention-programs-for-us-states
#6
Cora Peterson, Curtis Florence, Robert Thomas, Joanne Klevens
We assessed the US state-level budget and societal impact of implementing two child abuse and neglect (CAN) primary prevention programs. CAN cost estimates and data from two prevention programs (Child-Parent Centers and Nurse-Family Partnership) were combined with current population, cost, and CAN incidence data by US state. A cost-benefit mathematical model for each program by US state compared program costs with the future monetary value of benefits from reduced CAN. The models used a lifetime time horizon from government payer and societal perspectives...
July 22, 2017: Prevention Science: the Official Journal of the Society for Prevention Research
https://www.readbyqxmd.com/read/28735408/rapid-quantification-of-abscisic-acid-by-gc-ms-ms-for-studies-of-abiotic-stress-response
#7
Paul E Verslues
Drought and low water potential induce large increases in Abscisic Acid (ABA ) content of plant tissue. This increased ABA content is essential to regulate downstream stress resistance responses; however, the mechanisms regulating ABA accumulation are incompletely known. Thus, the ability to accurately quantify ABA at high throughput and low cost is important for plant stress research. We have combined and modified several previously published protocols to establish a rapid ABA analysis protocol using gas chromatography-tandem mass spectrometry (GC-MS/MS)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735406/detection-of-free-polyamines-in-plants-subjected-to-abiotic-stresses-by-high-performance-liquid-chromatography-hplc
#8
Xiaoqing Gong, Ji-Hong Liu
High-performance liquid chromatography (HPLC) is a sensitive, rapid, and accurate technique to detect and characterize various metabolites from plants. The metabolites are extracted with different solvents and eluted with appropriate mobile phases in a designed HPLC program. Polyamines are known to accumulate under abiotic stress conditions in various plant species and thought to provide protection against oxidative stress by scavenging reactive oxygen species. Here, we describe a common method to detect the free polyamines in plant tissues both qualitatively and quantitatively...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735402/determination-of-total-sulfur-sulfate-sulfite-thiosulfate-and-sulfolipids-in-plants
#9
Assylay Kurmanbayeva, Galina Brychkova, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, Moshe Sagi
In response to oxidative stress the biosynthesis of the ROS scavenger, glutathione is induced. This requires the induction of the sulfate reduction pathway for an adequate supply of cysteine, the precursor for glutathione. Cysteine also acts as the sulfur donor for the sulfuration of the molybdenum cofactor, crucial for the last step of ABA biosynthesis. Sulfate and sulfite are, respectively, the precursor and intermediate for cysteine biosynthesis and there is evidence for stress-induced sulfate uptake and further downstream, enhanced sulfite generation by 5'-phosphosulfate (APS) reductase (APR, EC 1...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735401/determination-of-enzymes-associated-with-sulfite-toxicity-in-plants-kinetic-assays-for-so-apr-sir-and-in-gel-sir-activity
#10
Galina Brychkova, Assylay Kurmanbayeva, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, Moshe Sagi
The amino acid cysteine plays a major role in plant response to abiotic stress by being the donor of elemental sulfur for the sulfuration of the molybdenum cofactor, otherwise the last step of ABA biosynthesis, the oxidation of abscisic aldehyde, is inactivated. Additionally, cysteine serves as a precursor for the biosynthesis of glutathione, the reactive oxygen species scavenger essential for redox status homeostasis during stress. Cysteine is generated by the sulfate reductive pathway where sulfite oxidase (SO; EC 1...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735392/mining-and-quantifying-in-vivo-molecular-interactions-in-abiotic-stress-acclimation
#11
Thorsten Seidel, Derya Kirasi
Stress acclimation is initialized by sensing the stressor, transducing the signal, and inducing the response. In particular, the signal transduction is driven by protein-protein interactions and the response might involve de novo complex formation, shifts in subcellular localization and, thus, transportation that is mediated by other proteins. The investigation of protein-protein interactions and their regulation upon abiotic stress is crucial for a deeper understanding of the underlying mechanisms. FRET measurements by sensitized emission allow for the analysis of protein-protein interactions in real time and have a high potential to provide new insights into the regulation of protein-protein interaction with respect to subcellular localization and time...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735388/epigenetics-and-rna-processing-connections-to-drought-salt-and-aba
#12
Min May Wong, Geeng Loo Chong, Paul E Verslues
There have been great research advances in epigenetics, RNA splicing, and mRNA processing over recent years. In parallel, there have been many advances in abiotic stress and Abscisic Acid (ABA) signaling. Here we overview studies that have examined stress-induced changes in the epigenome and RNA processing as well as cases where disrupting these processes changes the plant response to abiotic stress. We also highlight some examples where specific connections of stress or ABA signaling to epigenetics or RNA processing have been found...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735369/ethylene-sensitivity-and-relative-air-humidity-regulate-root-hydraulic-properties-in-tomato-plants
#13
Monica Calvo-Polanco, Pablo Ibort, Sonia Molina, Juan Manuel Ruiz-Lozano, Angel María Zamarreño, Jose María García-Mina, Ricardo Aroca
The effect of ethylene and its precursor ACC on root hydraulic properties, including aquaporin expression and abundance, is modulated by relative air humidity and plant sensitivity to ethylene. Relative air humidity (RH) is a main factor contributing to water balance in plants. Ethylene (ET) is known to be involved in the regulation of root water uptake and stomatal opening although its role on plant water balance under different RH is not very well understood. We studied, at the physiological, hormonal and molecular levels (aquaporins expression, abundance and phosphorylation state), the plant responses to exogenous 1-aminocyclopropane-1-carboxylic acid (ACC; precursor of ET) and 2-aminoisobutyric acid (AIB; inhibitor of ET biosynthesis), after 24 h of application to the roots of tomato wild type (WT) plants and its ET-insensitive never ripe (nr) mutant, at two RH levels: regular (50%) and close to saturation RH...
July 22, 2017: Planta
https://www.readbyqxmd.com/read/28735343/exact-probabilities-for-the-indeterminacy-of-complex-networks-as-perceived-through-press-perturbations
#14
David Koslicki, Mark Novak
We consider the goal of predicting how complex networks respond to chronic (press) perturbations when characterizations of their network topology and interaction strengths are associated with uncertainty. Our primary result is the derivation of exact formulas for the expected number and probability of qualitatively incorrect predictions about a system's responses under uncertainties drawn form arbitrary distributions of error. Additional indices provide new tools for identifying which links in a network are most qualitatively and quantitatively sensitive to error, and for determining the volume of errors within which predictions will remain qualitatively determinate (i...
July 22, 2017: Journal of Mathematical Biology
https://www.readbyqxmd.com/read/28735334/detection-of-polioviruses-in-sewage-using-cell-culture-and-molecular-methods
#15
Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735303/acid-fast-bacterium-detection-and-identification-from-paraffin-embedded-tissues-using-a-pcr-pyrosequencing-method
#16
Jian R Bao, Richard B Clark, Ronald N Master, Kileen L Shier, Lynn L Eklund
AIMS: Acid-fast bacterium (AFB) identification from formalin-fixed paraffin-embedded (FFPE) tissues is challenging and may not be readily available to the clinical laboratory. A method to detect and identify AFB from FFPE tissues using PCR and pyrosequencing (PCR-Seq) was developed and evaluated. METHODS: The method was validated using spiked cell-clotted paraffin blocks before use with patients' specimens. DNA was extracted from tissue sections, and a 16S rRNA gene fragment was amplified and a signature sequence was produced on a PyroMark ID system...
July 22, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28735301/effect-of-faecal-calprotectin-assay-variability-on-the-management-of-inflammatory-bowel-disease-and-potential-role-of-faecal-s100a12
#17
Simon John Whitehead, Clare Ford, Rousseau Mariano Gama, Ala Ali, Brian McKaig, Jenna Louise Waldron, Helen Steed, Matthew James Brookes
AIMS: To prospectively evaluate whether between-assay variability of different faecal calprotectin (f-Cp) assays influences diagnostic accuracy for inflammatory bowel disease (IBD) in a cohort of patients with confirmed IBD and irritable bowel syndrome (IBS). To also evaluate the diagnostic accuracy of faecal S100A12 (f-S100A12) against f-Cp in the same patient cohort and assess whether f-S100A12 offers additional diagnostic value. METHODS: F-Cp using four commercially available f-Cp assays, f-S100A12 and blood biomarkers were measured in patients, recruited from the local IBD clinic, who had established IBS or active ulcerative colitis (UC) and Crohn's disease (CD)...
July 22, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28735292/pathogenesis-of-hypertrophic-cardiomyopathy-is-mutation-rather-than-disease-specific-a-comparison-of-the-cardiac-troponin-t-e163r-and-r92q-mouse-models
#18
Cecilia Ferrantini, Raffaele Coppini, Josè Manuel Pioner, Francesca Gentile, Benedetta Tosi, Luca Mazzoni, Beatrice Scellini, Nicoletta Piroddi, Annunziatina Laurino, Lorenzo Santini, Valentina Spinelli, Leonardo Sacconi, Pieter De Tombe, Rachel Moore, Jil Tardiff, Alessandro Mugelli, Iacopo Olivotto, Elisabetta Cerbai, Chiara Tesi, Corrado Poggesi
BACKGROUND: In cardiomyocytes from patients with hypertrophic cardiomyopathy, mechanical dysfunction and arrhythmogenicity are caused by mutation-driven changes in myofilament function combined with excitation-contraction (E-C) coupling abnormalities related to adverse remodeling. Whether myofilament or E-C coupling alterations are more relevant in disease development is unknown. Here, we aim to investigate whether the relative roles of myofilament dysfunction and E-C coupling remodeling in determining the hypertrophic cardiomyopathy phenotype are mutation specific...
July 22, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28735291/subclinical-atherosclerosis-cardiac-and-kidney-function-heart-failure-and-dementia-in-the-very-elderly
#19
Lewis H Kuller, Oscar L Lopez, John S Gottdiener, Dalane W Kitzman, James T Becker, Yuefang Chang, Anne B Newman
BACKGROUND: Heart failure (HF) and dementia are major causes of disability and death among older individuals. Risk factors and biomarkers of HF may be determinants of dementia in the elderly. We evaluated the relationship between biomarkers of cardiovascular disease and HF and risk of dementia and death. Three hypotheses were tested: (1) higher levels of high-sensitivity cardiac troponin T, N-terminal of prohormone brain natriuretic peptide, and cystatin C predict risk of death, cardiovascular disease, HF, and dementia; (2) higher levels of cardiovascular disease biomarkers are associated with increased risk of HF and then secondary increased risk of dementia; and (3) risk of dementia is lower among participants with a combination of lower coronary artery calcium, atherosclerosis, and lower high-sensitivity cardiac troponin T (myocardial injury)...
July 22, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28735290/membrane-tethered-metalloproteinase-expressed-by-vascular-smooth-muscle-cells-limits-the-progression-of-proliferative-atherosclerotic-lesions
#20
Richard H Barnes, Takeshi Akama, Miina K Öhman, Moon-Sook Woo, Julian Bahr, Stephen J Weiss, Daniel T Eitzman, Tae-Hwa Chun
BACKGROUND: The MMP (matrix metalloproteinase) family plays diverse and critical roles in directing vascular wall remodeling in atherosclerosis. Unlike secreted-type MMPs, a member of the membrane-type MMP family, MT1-MMP (membrane-type 1 MMP; MMP14), mediates pericellular extracellular matrix degradation that is indispensable for maintaining physiological extracellular matrix homeostasis. However, given the premature mortality exhibited by MT1-MMP-null mice, the potential role of the proteinase in atherogenesis remains elusive...
July 22, 2017: Journal of the American Heart Association
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