keyword
MENU ▼
Read by QxMD icon Read
search

WSSV

keyword
https://www.readbyqxmd.com/read/28213041/newly-identified-pctoll4-regulates-antimicrobial-peptide-expression-in-intestine-of-red-swamp-crayfish-procambarus-clarkii
#1
Ying Huang, Tingting Li, Min Jin, Shaowu Yin, Kai-Min Hui, Qian Ren
Tolls or Toll-like receptors (TLRs) have an essential role in initiating innate immune responses against pathogens. In this study, a novel Toll gene, PcToll4, was first identified from the intestinal transcriptome of the freshwater crayfish, Procambarus clarkii. The PcToll4 cDNA is 4849bp long with a 3036bp open reading frame that encodes a 1011-amino acid protein. PcToll4 contains a signal peptide, 13 LRR domains, 3 LRR TYP domains, 2 LRR CT domains, an LRR NT domain, a transmembrane region, and a TIR domain...
February 14, 2017: Gene
https://www.readbyqxmd.com/read/28212834/death-associated-protein-1-dap-1-positively-regulates-virus-replication-and-apoptosis-of-hemocytes-in-shrimp-marsupenaeus-japonicus
#2
Wen-Li Xia, Li-Hua Kang, Chang-Bin Liu, Cui-Jie Kang
Death-associated protein 1 (DAP1) is a small proline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. However, little knowledge is known about the homologue gene of DAP1 and its roles in the physiological process of invertebrates. In this paper, we report a novel function of DAP1 in the antivirus immunity of shrimp. A homologue gene of DAP1 was cloned from Marsupenaeus japonicus and named as Mjdap-1. The full-length of Mjdap-1 was 1761 bp with a 309 bp open reading frame that encoded 102 amino acids...
February 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28193450/heat-shock-70%C3%A2-kda-protein-cognate-5-involved-in-wssv-toleration-of-litopenaeus-vannamei
#3
Kai Yuan, Feng-Hua Yuan, Hong-Hui He, Hai-Tao Bi, Shao-Ping Weng, Jian-Guo He, Yi-Hong Chen
The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5...
February 10, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28181552/microrna-100-is-involved-in-shrimp-immune-response-to-white-spot-syndrome-virus-wssv-and-vibrio-alginolyticus-infection
#4
Zhi Wang, Fei Zhu
In this study, we discovered that shrimp miR-100 was up-regulated at 24 h after WSSV or Vibrio alginolyticus infection, confirming its participation in the innate immune system of shrimp. The anti-miRNA oligonucleotide (AMO-miR-100) was applied to inhibit the expression of miR-100. After AMO-miR-100 treatment, the shrimp was challenged with WSSV or V. alginolyticus. The knockdown of miR-100 expression decreased the mortality of WSSV-infected shrimp from 24 h to 72 h post-infection and enhanced the mortality of V...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28179524/two-white-spot-syndrome-virus-mirnas-target-dorsal-to-promote-virus-infection-in-shrimp-marsupenaeus-japonicas
#5
Qian Ren, Xin Huang, Yalei Cui, Jiejie Sun, Wen Wang, Xiaobo Zhang
In eukaryotes, microRNAs (miRNAs) serve as regulators of many biological processes, including virus infection. A miRNA can generally target diverse genes during virus-host interactions. However, regulation of gene expression by multiple miRNAs has not yet been extensively explored during virus infection. This study found that the Spz-Toll-Dorsal-ALF signaling pathway played a very important role in antiviral immunity in shrimp (Marsupenaeus japonicus) against invasion of white spot syndrome virus (WSSV). Dorsal, the central gene in Toll pathway, was targeted by two viral miRNAs (WSSV-miR-N13 and WSSV-miR-N23) during WSSV infection...
February 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28141496/anti-melanization-mechanism-of-the-white-spot-syndrome-viral-protein-wssv453-via-interaction-with-shrimp-propo-activating-enzyme-pmproppae2
#6
Jantiwan- Sutthangkul, Piti- Amparyup, Jai Hoon Eum, Michael R Strand, Anchalee Tassanakajon
Inhibition of the host melanization reaction, activated by the prophenoloxidase activating (proPO) system, is one of the crucial evasion strategies of pathogens. Recently, the shrimp pathogen, white spot syndrome virus (WSSV), was found to inhibit melanization in the shrimp, Penaeus monodon. The viral protein WSSV453 was previously shown to interact with PO-activating enzyme 2 (PmPPAE2) and reported to be involved in suppressing the shrimp melanization response after WSSV infection. Here, we characterized how WSSV453 inhibits melanization...
January 28, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28126554/triosephosphate-isomerase-tpi-facilitates-the-replication-of-wssv-in-exopalaemon-carinicauda
#7
Fei Liu, Shihao Li, Guangxing Liu, Fuhua Li
Triosephosphate isomerase (TPI) is a vital enzyme in the glycolytic pathway, which can catalyze the interconversion of glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP). DHAP is involved in lipid metabolism and phospholipid synthesis. In order to know the role of TPI in WSSV infection to prawn, we cloned the full length cDNA of triosephosphate isomerase gene (EcTPI) from Exopalaemon carinicauda, and its function during WSSV infection was analyzed. EcTPI transcripts were widely distributed in all tissues, but showed relatively higher expression levels in the gill and epidermis...
January 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28105747/virulence-and-genotypes-of-white-spot-syndrome-virus-infecting-pacific-white-shrimp-litopenaeus-vannamei-in-north-western-mexico
#8
J Ramos-Paredes, J M Grijalva-Chon, J C Ibarra-Gámez
White spot syndrome virus (WSSV) has caused substantial global economic impact on aquaculture, and it has been determined that strains can vary in virulence. In this study, the effect of viral load was evaluated by infecting Litopenaeus vannamei with 10-fold serial dilution of tissue infected with strain WSSV Mx-H, and the virulence of four WSSV strains from north-western Mexico was assessed along with their variable number of tandem repeat (VNTR) genotypes in ORF75, ORF94 and ORF125. The LD50 of the Mx-H strain was a dilution dose of 10(-7...
March 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28094323/gene-set-based-association-analyses-for-the-wssv-resistance-of-pacific-white-shrimp-litopenaeus-vannamei
#9
Yang Yu, Jingwen Liu, Fuhua Li, Xiaojun Zhang, Chengsong Zhang, Jianhai Xiang
White Spot Syndrome Virus (WSSV) is regarded as a virus with the strongest pathogenicity to shrimp. For the threshold trait such as disease resistance, marker assisted selection (MAS) was considered to be a more effective approach. In the present study, association analyses of single nucleotide polymorphisms (SNPs) located in a set of immune related genes were conducted to identify markers associated with WSSV resistance. SNPs were detected by bioinformatics analysis on RNA sequencing data generated by Illimina sequencing platform and Roche 454 sequencing technology...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28089897/crustacean-hyperglycemic-hormones-directly-modulate-the-immune-response-of-hemocytes-in-shrimp-litopenaeus-vannamei
#10
Lin Wang, Hao Chen, Jianchao Xu, Qingsong Xu, Mengqiang Wang, Depeng Zhao, Lingling Wang, Linsheng Song
A robust immune response against invading pathogens is crucial for host to survive, which depends greatly on the well balance of metabolism. Increasing evidence has indicated that some metabolic hormones, such as insulin, could modulate immune responses directly. Crustacean hyperglycemic hormone (CHH) family is a group of ecdysozoans-specific peptide hormone involved in glucose metabolism and other biological events. In the present study, two members of CHH family (designated as LvCHH I and LvCHH II) in shrimp Litopenaeus vannamei with one and two crustacean neurohormone domains respectively were chosen to investigate their putative modulatory roles in both glucose metabolism and immune response...
January 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28077637/role-of-litopenaeus-vannamei-yin-yang-1-in-the-regulation-of-the-white-spot-syndrome-virus-immediate-early-gene-ie1
#11
Ping-Han Huang, Ting-Yi Huang, Pei-Si Cai, Li-Kwan Chang
: Yin Yang 1 (YY1) is a multifunctional zinc-finger transcription factor that regulates many key cellular processes. In this study, we report the cloning of YY1 from shrimp, Litopenaeus vannamei, (LvYY1). The study shows that LvYY1 is ubiquitously expressed in shrimp tissues, and knockdown of LvYY1 expression by dsRNA injection in WSSV-infected shrimp reduced both mRNA levels of the WSSV immediate-early gene, ie1, as well as overall copy numbers of the WSSV genome. The cumulative mortality of infected shrimp also declined with LvYY1 dsRNA injection...
January 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28069434/identification-and-functional-analysis-of-a-tep-gene-from-a-crustacean-reveals-its-transcriptional-regulation-mediated-by-nf-%C3%AE%C2%BAb-and-jnk-pathways-and-its-broad-protective-roles-against-multiple-pathogens
#12
Chaozheng Li, Haoyang Li, Bang Xiao, Yonggui Chen, Sheng Wang, Kai Lǚ, Bin Yin, Sedong Li, Jianguo He
Thioester-containing proteins (TEPs) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. In the current study, a TEP gene homologous to insect TEPs (iTEP) from the crustacean Litopenaeus vannamei, named LvTEP1, is cloned and functionally characterized. The open reading frame (ORF) of LvTEP1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kDa LvTEP1, which is most similar to other TEPs from insects, contains some conserved sequence features, including a N-terminal signal peptide, a canonical thioester (TE) motif, and a C-terminal distinctive cysteine signature...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28065702/molecular-characterization-of-a-p38-mitogen-activated-protein-kinase-gene-from-scylla-paramamosain-and-its-expression-profiles-during-pathogenic-challenge
#13
Zehui Yu, Yi Geng, Anming Huang, Kaiyu Wang, Xiaoli Huang, Defang Chen, Yangping Ou, Jun Wang
A novel p38 MAPK gene from S. paramamosain was cloned and characterized by rapid amplification of cDNA ends (RACE) technology. S. paramamosain p38 (Sp-p38) MAPK gene consists of an open reading frame of 1095bp encoding a 365-amino-acid protein, which showed close phylogenetic relationship to Litopenaeus vannamei p38 MAPK. The tissue distribution patterns showed that Sp-p38 MAPK was widely expressed in all examined tissues, with the highest expression in hemocytes and intestines. The expression levels of Sp-p38 MAPK in hemocytes was up-regulated post-stimulation, which reached the peak at 6h and 12h after bacteria (S...
January 6, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28046005/field-usable-lateral-flow-immunoassay-for-the-rapid-detection-of-white-spot-syndrome-virus-wssv
#14
Prabir Kumar Kulabhusan, Jyutika M Rajwade, Vimal Sugumar, Gani Taju, A S Sahul Hameed, Kishore M Paknikar
BACKGROUND: White spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagnostic method for the detection of WSSV infection is imperative to prevent huge economic losses...
2017: PloS One
https://www.readbyqxmd.com/read/28031362/interaction-of-the-small-gtpase-cdc42-with-arginine-kinase-restricts-white-spot-syndrome-virus-in-shrimp
#15
Ji-Dong Xu, Hai-Shan Jiang, Tian-Di Wei, Ke-Yi Zhang, Xian-Wei Wang, Xiao-Fan Zhao, Jin-Xing Wang
Many types of small GTPases are widely expressed in eukaryotes and have different functions. As a crucial member of the Rho GTPase family, Cdc42 serves a number of functions, such as regulating cell growth, migration, and cell movement. Several RNA viruses employ Cdc42-hijacking tactics in their target cell entry processes. However, the function of Cdc42 in shrimp antiviral immunity is not clear. In this study, we identified a Cdc42 protein in the kuruma shrimp (Marsupenaeus japonicus) and named it MjCdc42...
March 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28027319/scavenger-receptor-c-mediates-phagocytosis-of-white-spot-syndrome-virus-and-restricts-virus-proliferation-in-shrimp
#16
Ming-Chong Yang, Xiu-Zhen Shi, Hui-Ting Yang, Jie-Jie Sun, Ling Xu, Xian-Wei Wang, Xiao-Fan Zhao, Jin-Xing Wang
Scavenger receptors are an important class of pattern recognition receptors that play several important roles in host defense against pathogens. The class C scavenger receptors (SRCs) have only been identified in a few invertebrates, and their role in the immune response against viruses is seldom studied. In this study, we firstly identified an SRC from kuruma shrimp, Marsupenaeus japonicus, designated MjSRC, which was significantly upregulated after white spot syndrome virus (WSSV) challenge at the mRNA and protein levels in hemocytes...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/28025158/characterization-and-function-analysis-of-interleukin-1-receptor-associated-kinase-1-irak-1-from-fenneropenaeus-penicillatus
#17
Youhou Xu, Yucong Huang, Shuanghu Cai
The interleukin-1 receptor-associated kinase-1 (IRAK-1) is an important adapter protein which links downstream of MyD88, and involved in the complex composed of MyD88 and TRAF6 to activate TLRs signaling pathway. In this study, an IRAK-1 homolog (FpIRAK-1) was cloned from the red tail shrimp Fenneropenaeus penicillatus. The ORF of FpIRAK-1 consisted of 2874 bp encoding a protein of 957 amino acids which contains a death domain (DD) and a catalytic domain of serine/threonine kinases (STKc). Homology analysis revealed that the predicted amino acid sequence of FpIRAK-1 shared 71% similarities with IRAK-1 of Litopenaeus vannamei...
February 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28013454/comparative-genomic-analysis-of-three-white-spot-syndrome-virus-isolates-of-different-virulence
#18
Fang Li, Meiling Gao, Limei Xu, Feng Yang
Three white spot syndrome virus (WSSV) isolates of different virulence were identified in our previous study, the high-virulent strain WSSV-CN01, the moderate-virulent strain WSSV-CN02 and the low-virulent strain WSSV-CN03. In this study, the genomes of these three WSSV isolates were sequenced, annotated and compared. The genome sizes for WSSV-CN01, WSSV-CN02, and WSSV-CN03 are 309,286, 294,261, and 284,148 bp, bearing 177, 164, and 154 putative protein-coding genes, respectively. The genomic variations including insertions, deletions, and substitutions were investigated...
December 24, 2016: Virus Genes
https://www.readbyqxmd.com/read/27992657/protection-of-penaeus-monodon-against-white-spot-syndrome-by-continuous-oral-administration-of-a-low-concentration-of-bacillus-subtilis-spores-expressing-the-vp28-antigen
#19
K-C Pham, H T T Tran, C Van Doan, P H Le, A T Van Nguyen, H A Nguyen, H A Hong, S M Cutting, T-N Phan
: In this study, Bacillus subtilis spores expressing a chimeric protein, CotB-VP28, were used as a probiotic vaccine to protect black tiger shrimps (Penaeus monodon) against white spot syndrome virus (WSSV) infection. Oral administration of pellets coated with CotB-VP28 spores (at ≥1 × 10(9 ) CFU per g pellet) to shrimps induced immune-relating phenoloxydase activity (PO) in shrimps after 14 days of feeding (prior challenge) and at day 3 post challenge (1·26 and 1·70 fold increase respectively)...
December 19, 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/27974730/highly-sensitive-detection-of-low-abundance-of-white-spot-syndrome-virus-by-a-pre-amplification-pcr-method
#20
Xiaoming Pan, Yanfang Zhang, Xuejiao Sha, Jing Wang, Jing Li, Ping Dong, Xingguo Liang
White spot syndrome virus (WSSV) is a major threat to shrimp farming industry and so far there's no effective therapeutic way to it, thus early diagnostic of WSSV is of great importance. However, at the early stage of infection the extremely low-abundance of WSSV DNA challenged the detection sensitivity and accuracy of PCR. To effectively detect low-abundance of WSSV, here we developed a pre-amplification PCR (pre-amp PCR) method to amplify trace amounts of WSSV DNA from massive background genomic DNA. Combining with normal specific PCR, ten copies of target WSSV gene were detected from ~10¹⁰ magnitude of backgrounds...
December 14, 2016: Journal of Microbiology and Biotechnology
keyword
keyword
63376
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"