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https://www.readbyqxmd.com/read/28330853/noncoding-mirnas-bridge-virus-infection-and-host-autophagy-in-shrimp-in-vivo
#1
Yaodong He, Yuechao Sun, Xiaobo Zhang
Autophagy is known to be involved in viral infection. However, the relationship between autophagy and viral infection has not been characterized. MicroRNAs (miRNAs), important regulators of gene transcription, play central roles in many biologic processes, including autophagy and viral infection. It was therefore of interest to investigate the miRNAs regulating viral infection and host autophagy. In this study the viral infection (white spot syndrome virus, WSSV) correlated positively with the host autophagy in shrimp...
March 22, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28315393/tumor-necrosis-factor-receptor-associated-factor-6-traf6-participates-in-peroxinectin-gene-expression-in-fenneropenaeus-penicillatus
#2
Shuanghu Cai, Yucong Huang, Bei Wang, Jichang Jian, Youhou Xu
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is an important cytoplasm signal adaptor that mediates signals activated by tumor necrosis factor receptor (TNFR) superfamily and the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. In the study, the full-length cDNA of a TRAF6 homolog (FpTRAF6) was identified from Fenneropenaeus penicillatus. The full-length cDNA of FpTRAF6 is 2033 bp long, with an open reading frame (ORF) encoding a putative protein of 594 amino acids, including a RING type Zinc finger, two TRAF-type Zinc fingers, and a conserved C-terminal meprin and TRAF homology (MATH) domain...
March 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28235638/immune-response-and-antioxidant-status-of-portunus-trituberculatus-inoculated-with-pathogens
#3
Xianyun Ren, Jianjian Lv, Baoquan Gao, Jian Li, Ping Liu
The white spot syndrome virus (WSSV), Vibrio parahaemolyticus and V. alginolyticus are serious epidemic pathogen affecting the cultured Portunus trituberculatus and resulted in severe economic losses to local farmers. The immune and antioxidant systems are believed to be closely involved in host responses to pathogens in aerobic animals, including crustaceans. In order to explore such host-pathogen interactions in the early stage of infection in P. trituberculatus, the mRNA transcript levels of six key immune-related genes (proPO, α2M, crustin, lysozyme, NOS, and NOX) and three key antioxidant-related genes (CuZnSOD, CAT, and GPx) and their corresponding enzymatic activity were investigated in response to challenge with the three pathogens...
February 21, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28222204/comparative-transcriptome-and-potential-antiviral-signaling-pathways-analysis-of-the-gills-in-the-red-swamp-crayfish-procambarus-clarkii-infected-with-white-spot-syndrome-virus-wssv
#4
Zhi-Qiang Du, Yan-Hui Jin
Red swamp crayfish is an important model organism for research of the invertebrate innate immunity mechanism. Its excellent disease resistance against bacteria, fungi, and viruses is well-known. However, the antiviral mechanisms of crayfish remain unclear. In this study, we obtained high-quality sequence reads from normal and white spot syndrome virus (WSSV)-challenged crayfish gills. For group normal (GN), 39,390,280 high-quality clean reads were randomly assembled to produce 172,591 contigs; whereas, 34,011,488 high-quality clean reads were randomly assembled to produce 182,176 contigs for group WSSV-challenged (GW)...
February 20, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28216323/cdna-cloning-and-expression-analysis-of-a-phosphopyruvate-hydratase-gene-from-the-chinese-shrimp-fenneropenaeus-chinensis
#5
Xupeng Li, Xianhong Meng, Kun Luo, Sheng Luan, Baoxiang Cao, Jie Kong
In the present study a cDNA encoding a phosphopyruvate hydratase (enolase) was cloned from the muscle of the Chinese shrimp (Fenneropenaeus chinensis) and named as FcEnolase. The cDNA of FcEnolase encoded a protein of 434 amino acid residues with a molecular mass 47.22 kDa. The residues 342-355 constituted the signature motif "LLLKVNQIGSVTES". A SNP locus (C96T) in the ORF at 96 bp was identified. The results showed that the FcEnolase was a conserved gene. In the normal F. chinensis, the mRNA level in the muscle was much higher (P < 0...
February 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28216094/fusion-of-flagellin-2-with-bivalent-white-spot-syndrome-virus-vaccine-increases-survival-in-freshwater-shrimp
#6
Hansam Cho, Na Hye Park, Yuyeon Jang, Yong-Dae Gwon, Yeondong Cho, Yoon-Ki Heo, Ki-Hoon Park, Hee-Jung Lee, Tae Jin Choi, Young Bong Kim
Despite large economic losses attributable to white spot syndrome virus (WSSV), an infectious pathogen of penaeid shrimp and other crustaceans worldwide, no efficient vaccines or antiviral agents to control the virus are available at present. Here, we designed and constructed baculovirus-based vaccines delivering genes encoding the WSSV envelope proteins, VP28 and VP19. To enhance the immunogenicity of the baculovirus-based vaccine, we fused a Salmonella typhimurium flagellin 2 (FL2) gene with VP28 or VP19 gene...
March 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28213041/newly-identified-pctoll4-regulates-antimicrobial-peptide-expression-in-intestine-of-red-swamp-crayfish-procambarus-clarkii
#7
Ying Huang, Tingting Li, Min Jin, Shaowu Yin, Kai-Min Hui, Qian Ren
Tolls or Toll-like receptors (TLRs) have an essential role in initiating innate immune responses against pathogens. In this study, a novel Toll gene, PcToll4, was first identified from the intestinal transcriptome of the freshwater crayfish, Procambarus clarkii. The PcToll4 cDNA is 4849bp long with a 3036bp open reading frame that encodes a 1011-amino acid protein. PcToll4 contains a signal peptide, 13 LRR domains, 3 LRR TYP domains, 2 LRR CT domains, an LRR NT domain, a transmembrane region, and a TIR domain...
April 30, 2017: Gene
https://www.readbyqxmd.com/read/28212834/death-associated-protein-1-dap-1-positively-regulates-virus-replication-and-apoptosis-of-hemocytes-in-shrimp-marsupenaeus-japonicus
#8
Wen-Li Xia, Li-Hua Kang, Chang-Bin Liu, Cui-Jie Kang
Death-associated protein 1 (DAP1) is a small proline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. However, little knowledge is known about the homologue gene of DAP1 and its roles in the physiological process of invertebrates. In this paper, we report a novel function of DAP1 in the antivirus immunity of shrimp. A homologue gene of DAP1 was cloned from Marsupenaeus japonicus and named as Mjdap-1. The full-length of Mjdap-1 was 1761 bp with a 309 bp open reading frame that encoded 102 amino acids...
February 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28193450/heat-shock-70%C3%A2-kda-protein-cognate-5-involved-in-wssv-toleration-of-litopenaeus-vannamei
#9
Kai Yuan, Feng-Hua Yuan, Hong-Hui He, Hai-Tao Bi, Shao-Ping Weng, Jian-Guo He, Yi-Hong Chen
The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5...
February 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28181552/microrna-100-is-involved-in-shrimp-immune-response-to-white-spot-syndrome-virus-wssv-and-vibrio-alginolyticus-infection
#10
Zhi Wang, Fei Zhu
In this study, we discovered that shrimp miR-100 was up-regulated at 24 h after WSSV or Vibrio alginolyticus infection, confirming its participation in the innate immune system of shrimp. The anti-miRNA oligonucleotide (AMO-miR-100) was applied to inhibit the expression of miR-100. After AMO-miR-100 treatment, the shrimp was challenged with WSSV or V. alginolyticus. The knockdown of miR-100 expression decreased the mortality of WSSV-infected shrimp from 24 h to 72 h post-infection and enhanced the mortality of V...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28179524/two-white-spot-syndrome-virus-mirnas-target-dorsal-to-promote-virus-infection-in-shrimp-marsupenaeus-japonicas
#11
Qian Ren, Xin Huang, Yalei Cui, Jiejie Sun, Wen Wang, Xiaobo Zhang
In eukaryotes, microRNAs (miRNAs) serve as regulators of many biological processes, including virus infection. A miRNA can generally target diverse genes during virus-host interactions. However, regulation of gene expression by multiple miRNAs has not yet been extensively explored during virus infection. This study found that the Spz-Toll-Dorsal-ALF signaling pathway played a very important role in antiviral immunity in shrimp (Marsupenaeus japonicus) against invasion of white spot syndrome virus (WSSV). Dorsal, the central gene in Toll pathway, was targeted by two viral miRNAs (WSSV-miR-N13 and WSSV-miR-N23) during WSSV infection...
February 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28141496/anti-melanization-mechanism-of-the-white-spot-syndrome-viral-protein-wssv453-via-interaction-with-shrimp-propo-activating-enzyme-pmproppae2
#12
Jantiwan- Sutthangkul, Piti- Amparyup, Jai Hoon Eum, Michael R Strand, Anchalee Tassanakajon
Inhibition of the host melanization reaction, activated by the prophenoloxidase activating (proPO) system, is one of the crucial evasion strategies of pathogens. Recently, the shrimp pathogen, white spot syndrome virus (WSSV), was found to inhibit melanization in the shrimp, Penaeus monodon. The viral protein WSSV453 was previously shown to interact with PO-activating enzyme 2 (PmPPAE2) and reported to be involved in suppressing the shrimp melanization response after WSSV infection. Here, we characterized how WSSV453 inhibits melanization...
January 28, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28126554/triosephosphate-isomerase-tpi-facilitates-the-replication-of-wssv-in-exopalaemon-carinicauda
#13
Fei Liu, Shihao Li, Guangxing Liu, Fuhua Li
Triosephosphate isomerase (TPI) is a vital enzyme in the glycolytic pathway, which can catalyze the interconversion of glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP). DHAP is involved in lipid metabolism and phospholipid synthesis. In order to know the role of TPI in WSSV infection to prawn, we cloned the full length cDNA of triosephosphate isomerase gene (EcTPI) from Exopalaemon carinicauda, and its function during WSSV infection was analyzed. EcTPI transcripts were widely distributed in all tissues, but showed relatively higher expression levels in the gill and epidermis...
January 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28105747/virulence-and-genotypes-of-white-spot-syndrome-virus-infecting-pacific-white-shrimp-litopenaeus-vannamei-in-north-western-mexico
#14
J Ramos-Paredes, J M Grijalva-Chon, J C Ibarra-Gámez
White spot syndrome virus (WSSV) has caused substantial global economic impact on aquaculture, and it has been determined that strains can vary in virulence. In this study, the effect of viral load was evaluated by infecting Litopenaeus vannamei with 10-fold serial dilution of tissue infected with strain WSSV Mx-H, and the virulence of four WSSV strains from north-western Mexico was assessed along with their variable number of tandem repeat (VNTR) genotypes in ORF75, ORF94 and ORF125. The LD50 of the Mx-H strain was a dilution dose of 10(-7...
March 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28094323/gene-set-based-association-analyses-for-the-wssv-resistance-of-pacific-white-shrimp-litopenaeus-vannamei
#15
Yang Yu, Jingwen Liu, Fuhua Li, Xiaojun Zhang, Chengsong Zhang, Jianhai Xiang
White Spot Syndrome Virus (WSSV) is regarded as a virus with the strongest pathogenicity to shrimp. For the threshold trait such as disease resistance, marker assisted selection (MAS) was considered to be a more effective approach. In the present study, association analyses of single nucleotide polymorphisms (SNPs) located in a set of immune related genes were conducted to identify markers associated with WSSV resistance. SNPs were detected by bioinformatics analysis on RNA sequencing data generated by Illimina sequencing platform and Roche 454 sequencing technology...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28089897/crustacean-hyperglycemic-hormones-directly-modulate-the-immune-response-of-hemocytes-in-shrimp-litopenaeus-vannamei
#16
Lin Wang, Hao Chen, Jianchao Xu, Qingsong Xu, Mengqiang Wang, Depeng Zhao, Lingling Wang, Linsheng Song
A robust immune response against invading pathogens is crucial for host to survive, which depends greatly on the well balance of metabolism. Increasing evidence has indicated that some metabolic hormones, such as insulin, could modulate immune responses directly. Crustacean hyperglycemic hormone (CHH) family is a group of ecdysozoans-specific peptide hormone involved in glucose metabolism and other biological events. In the present study, two members of CHH family (designated as LvCHH I and LvCHH II) in shrimp Litopenaeus vannamei with one and two crustacean neurohormone domains respectively were chosen to investigate their putative modulatory roles in both glucose metabolism and immune response...
January 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28077637/role-of-litopenaeus-vannamei-yin-yang-1-in-the-regulation-of-the-white-spot-syndrome-virus-immediate-early-gene-ie1
#17
Ping-Han Huang, Ting-Yi Huang, Pei-Si Cai, Li-Kwan Chang
Yin Yang 1 (YY1) is a multifunctional zinc finger transcription factor that regulates many key cellular processes. In this study, we report the cloning of YY1 from Litopenaeus vannamei shrimp (LvYY1). This study shows that LvYY1 is ubiquitously expressed in shrimp tissues, and knockdown of LvYY1 expression by double-stranded RNA (dsRNA) injection in white spot syndrome virus (WSSV)-infected shrimp reduced both mRNA levels of the WSSV immediate early gene ie1 as well as overall copy numbers of the WSSV genome...
March 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28069434/identification-and-functional-analysis-of-a-tep-gene-from-a-crustacean-reveals-its-transcriptional-regulation-mediated-by-nf-%C3%AE%C2%BAb-and-jnk-pathways-and-its-broad-protective-roles-against-multiple-pathogens
#18
Chaozheng Li, Haoyang Li, Bang Xiao, Yonggui Chen, Sheng Wang, Kai Lǚ, Bin Yin, Sedong Li, Jianguo He
Thioester-containing proteins (TEPs) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. In the current study, a TEP gene homologous to insect TEPs (iTEP) from the crustacean Litopenaeus vannamei, named LvTEP1, is cloned and functionally characterized. The open reading frame (ORF) of LvTEP1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kDa LvTEP1, which is most similar to other TEPs from insects, contains some conserved sequence features, including a N-terminal signal peptide, a canonical thioester (TE) motif, and a C-terminal distinctive cysteine signature...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28065702/molecular-characterization-of-a-p38-mitogen-activated-protein-kinase-gene-from-scylla-paramamosain-and-its-expression-profiles-during-pathogenic-challenge
#19
Zehui Yu, Yi Geng, Anming Huang, Kaiyu Wang, Xiaoli Huang, Defang Chen, Yangping Ou, Jun Wang
A novel p38 MAPK gene from S. paramamosain was cloned and characterized by rapid amplification of cDNA ends (RACE) technology. S. paramamosain p38 (Sp-p38) MAPK gene consists of an open reading frame of 1095bp encoding a 365-amino-acid protein, which showed close phylogenetic relationship to Litopenaeus vannamei p38 MAPK. The tissue distribution patterns showed that Sp-p38 MAPK was widely expressed in all examined tissues, with the highest expression in hemocytes and intestines. The expression levels of Sp-p38 MAPK in hemocytes was up-regulated post-stimulation, which reached the peak at 6h and 12h after bacteria (S...
March 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28046005/field-usable-lateral-flow-immunoassay-for-the-rapid-detection-of-white-spot-syndrome-virus-wssv
#20
Prabir Kumar Kulabhusan, Jyutika M Rajwade, Vimal Sugumar, Gani Taju, A S Sahul Hameed, Kishore M Paknikar
BACKGROUND: White spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagnostic method for the detection of WSSV infection is imperative to prevent huge economic losses...
2017: PloS One
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