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https://www.readbyqxmd.com/read/27919648/antiviral-action-of-the-antimicrobial-peptide-alfpm3-from-penaeus-monodon-against-white-spot-syndrome-virus
#1
Thanachai Methatham, Pakpoom Boonchuen, Phattarunda Jaree, Anchalee Tassanakajon, Kunlaya Somboonwiwat
The anti-lipopolysaccharide factor isoform 3 (ALFPm3), the antimicrobial peptide from Penaeus monodon, possesses antibacterial and antiviral activities. Although the mechanism of action of ALFPm3 against bacteria has been revealed but its antiviral mechanism is still unclear. To further study how the ALFPm3 exhibits antiviral activity against the enveloped virus, white spot syndrome virus (WSSV), the ALFPm3-interacting proteins from WSSV were sought and identified five ALFPm3-interacting proteins, WSSV186, WSSV189, WSSV395, WSSV458, and WSSV471...
December 2, 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27918999/effect-of-silver-nanoparticles-on-the-metabolic-rate-hematological-response-and-survival-of-juvenile-white-shrimp-litopenaeus-vannamei
#2
Karla Juarez-Moreno, Claudio Humberto Mejía-Ruiz, Fernando Díaz, Horacio Reyna-Verdugo, Ana Denisse Re, Edgar F Vazquez-Felix, Edna Sánchez-Castrejón, Josué D Mota-Morales, Alexey Pestryakov, Nina Bogdanchikova
White spot syndrome virus (WSSV) is highly lethal and contagious in shrimps; its outbreaks causes an economic crisis for aquaculture. Several attempts have been made to treat this disease; however, to date, there is no effective cure. Because of their antimicrobial activities, silver nanoparticles (AgNPs) are the most studied nanomaterial. Although the antiviral properties of AgNPs have been studied, their antiviral effect against viral infection in aquaculture has not been reported. The AgNPs tested herein are coated with polyvinylpyrrolidone (PVP) and possess multiple international certifications for their use in veterinary and human applications...
December 2, 2016: Chemosphere
https://www.readbyqxmd.com/read/27867019/potential-of-rnai-applications-to-control-viral-diseases-of-farmed-shrimp
#3
Ornchuma Itsathitphaisarn, Siripong Thitamadee, Wattana Weerachatyanukul, Kallaya Sritunyalucksana
Viral pathogens pose a primary threat to global shrimp aquaculture. Despite the urgent industry need for them, practical anti-viral control methods are unavailable due, in part, to lack of an adaptive immune response in crustaceans that renders conventional vaccination methods ineffective. One currently studied method of high interest for protecting shrimp against viral infection relies on the post-transcriptional gene silencing mechanism called RNA interference (RNAi) that is induced by gene-specific constructs of double stranded RNA (dsRNA)...
November 17, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27825947/characterization-of-micrornas-by-deep-sequencing-in-red-claw-crayfish-cherax-quadricarinatus-haematopoietic-tissue-cells-after-white-spot-syndrome-virus-infection
#4
Meng-Ru Zhao, Chuang Meng, Xiao-Lu Xie, Cheng-Hua Li, Hai-Peng Liu
White spot syndrome virus (WSSV) is one of the most prevalent and widespread viruses in both shrimp and crayfish aquaculture. MicroRNAs (miRNAs) are crucial post-transcriptional regulators and play critical roles in cell differentiation and proliferation, apoptosis, signal transduction and immunity. In this study, miRNA expression profiles were identified via deep sequencing in red claw crayfish Cherax quadricarinatus haematopoietic tissue (Hpt) cell cultures infected with WSSV at both early (i.e., 1 hpi) and late (i...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27822254/relationship-between-white-spot-syndrome-virus-wssv-loads-and-characterizations-of-water-quality-in-litopenaeus-vannamei-culture-ponds-during-the-tropical-storm
#5
J S Zhang, Z J Li, G L Wen, Y L Wang, L Luo, H J Zhang, H B Dong
An in-situ experiment was conducted to investigate the effect of tropical storm on the white spot syndrome virus (WSSV) loads in Litopenaeus vannamei rearing ponds. White spot syndrome virus loads, heterotrophic bacteria, Vibrio and water quality (including temperature, dissolved oxygen (DO), salinity, pH, NH4-N, and NO2-N) were continually monitored through one tropical storm. The WSSV loads decreased when tropical storm made landfall, and substantially increased when typhoon passed. The variation of WSSV loads was correlated with DO, temperature, heterotrophic bacteria count, and ammonia-N concentrations...
2016: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/27818342/a-c-type-lectin-mrlec-with-high-expression-in-intestine-is-involved-in-innate-immune-response-of-macrobrachium-rosenbergii
#6
Jinling Feng, Xin Huang, Min Jin, Yi Zhang, Tingting Li, Kaimin Hui, Qian Ren
C-type lectins (CTLs) are pattern-recognition proteins that play an important role in innate immunity of vertebrates and invertebrates. In this study, a lectin cDNA named MrLec was cloned and characterized from giant freshwater prawns (Macrobrachiun rosenbergii). The full-length cDNA of MrLec was 1431 bp, which contained an open reading frame of 1041 bp that encoded a protein with 346 amino acids. MrLec was found to contain a typical signal peptide of 18 amino acids and a single carbohydrate-recognition domain with 121 amino acids...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27815206/white-shrimp-litopenaeus-vannamei-that-have-received-fucoidan-exhibit-a-defense-against-vibrio-alginolyticus-and-wssv-despite-their-recovery-of-immune-parameters-to-background-levels
#7
Yu-Yuan Chen, Suwaree Kitikiew, Su-Tuen Yeh, Jiann-Chu Chen
White shrimp Litopenaeus vannamei receiving fucoidan at 2, 6, and 10 μg g(-1) after 0-144 h or 0-120 h were examined for immune parameters (haemograms, phenoloxidase activity, respiratory burst, and superoxide dismutase activity), proliferation of haemocyte in the haematopoietic tissue (HPT), gene expression, and phagocytic activity and clearance efficiency to Vibrio alginolyticus. Immune parameters and mitotic index of HPT increased after 3-24 h, reached their maxima after 48-72 h, and returned to background values after 144 h...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27815197/shrimp-stat-was-hijacked-by-white-spot-syndrome-virus-immediate-early-protein-ie1-involved-in-modulation-of-viral-genes
#8
Defu Yao, Lingwei Ruan, Huasong Lu, Hong Shi, Xun Xu
STATs are a family of transcription factors that regulate a cascade of cellular processes including cell growth, differentiation, apoptosis and immune responses. However, they are usually targeted by viruses to assist infection. In this study, we identified that white spot syndrome virus (WSSV) immediate-early protein IE1 interacted with Litopenaeus vannamei STAT (LvSTAT) and thereby led to its phosphorylation activation. In addition, we demonstrated that LvSTAT could bind to the promoters of the viral immediate-early genes wsv051 and ie1 through STAT-binding motifs in vitro and vivo, allowing the enhancement of their promoters' activities...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27756566/the-expression-and-purification-of-wssv134-from-white-spot-syndrome-virus-and-its-inhibitory-effect-on-caspase-activity-from-penaeus-monodon
#9
Thanunthon Bowornsakulwong, Walaiporn Charoensapsri, Triwit Rattanarojpong, Pongsak Khunrae
WSSV134 or VP36A protein of white spot syndrome virus was previously reported to be able to reduce apoptosis in Sf-9 cells transfected with caspase of Penaeus monodon (PmCasp). The protein was therefore believed to have a role in supporting the survival of WSSV inside the host cells during infection. However, the anti-apoptosis activity of WSSV134 involved in the inhibition of PmCasp is still unclear. In this study, we produced a recombinant WSSV134 (rWSSV134) and tested for its ability to inhibit PmCasp in vitro...
October 15, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27744059/effect-of-mixed-bacillus-spp-isolated-from-pustulose-ark-anadara-tuberculosa-on-growth-survival-viral-prevalence-and-immune-related-gene-expression-in-shrimp-litopenaeus-vannamei
#10
Ana Claudia Sánchez-Ortiz, Carlos Angulo, Antonio Luna-González, Píndaro Álvarez-Ruiz, José Manuel Mazón-Suástegui, Ángel Isidro Campa-Córdova
The widespread overuse of antibiotics in aquaculture has led to the emergence of antibiotic-resistance shrimp pathogens, the negative impact on shrimp gut microbiota, and the presence of antimicrobial residues in aquaculture products, with negative consequences on human health. Alternatively, probiotics have positive effects on immunological responses and productive performance of aquatic animals. In this study, three probiotic bacteria, (Bacillus licheniformis MAt32, B. subtilis MAt43 and B. subtilis subsp...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27648859/pellino-1-derived-cationic-antimicrobial-prawn-peptide-bactericidal-activity-toxicity-and-mode-of-action
#11
Gayathri Ravichandran, Venkatesh Kumaresan, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Munuswamy-Ramanujam Ganesh, Arun Mahesh, Arunkumar Dhayalan, Mukesh Pasupuleti, Jesu Arockiaraj
The antimicrobial peptides (AMPs) are multifunctional molecules which represent significant roles in the innate immune system. These molecules have been well known for decades because of their role as natural antibiotics in both invertebrates and vertebrates. The development of multiple drug resistance against conventional antibiotics brought a greater focus on AMPs in recent years. The cationic peptides, in particular, proven as host defense peptides and are considered as effectors of innate immunity. Among the various innate immune molecules, functions of pellino-1 (Peli-1) have been recently studied for its remarkable role in specific immune functions...
October 2016: Molecular Immunology
https://www.readbyqxmd.com/read/27631372/the-role-of-cytokine-pf4-in-the-antiviral-immune-response-of-shrimp
#12
Yulei Chen, Jiao Cao, Xiaobo Zhang
During viral infection in vertebrates, cytokines play important roles in the host defense against the virus. However, the function of cytokines in invertebrates has not been well characterized. In this study, shrimp cytokines involved in viral infection were screened using a cytokine antibody microarray. The results showed that three cytokines, the Fas receptor (Fas), platelet factor 4 (PF4) and interleukin-22 (IL-22), were significantly upregulated in the white spot syndrome virus (WSSV)-challenged shrimp, suggesting that these cytokines played positive regulatory roles in the immune response of shrimp against the virus...
2016: PloS One
https://www.readbyqxmd.com/read/27620819/c-type-lectin-mrctl-from-the-giant-freshwater-prawn-macrobrachium-rosenbergii-participates-in-innate-immunity
#13
Xin Huang, Jin-Ling Feng, Min Jin, Qian Ren, Wen Wang
C-type lectins (CTLs) play important roles in the innate immunity of invertebrates. In this study, a novel CTL with a single carbohydrate recognition domain (CRD) containing an EPN (Glu-Pro-Asn) motif was identified from the giant freshwater prawn Macrobrachium rosenbergii. This CTL was designated as MrCTL. The cDNA of MrCTL is 1788 bp with a 657 bp open reading frame that encodes a protein of 218 amino acids. The cDNA and genome sequences of MrCTL show a polymorphism that leads to MrCTL isoforms. MrCTL was highly expressed in the gills and intestine of normal prawn, and its transcription increased after Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge...
September 9, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27620817/differential-expression-of-micrornas-of-litopenaeus-vannamei-in-response-to-different-virulence-wssv-infection
#14
Xinying Sun, Qing-Hui Liu, Bing Yang, Jie Huang
WSSV is one of the most harmful pathogeny in the pacific white shrimp, and genetic variations caused the strains of different virulence. MicroRNAs (miRNAs) involved in the regulation of virus defense. To understand the different virulence of WSSV on miRNA expression in Litopeneaus vannamei, the deep sequencing was performed to compare two small RNA libraries prepared from hepatopancreas of Litopeneaus vannamei infected with normal-virulence or low-virulence WSSV. Approximately 29,398,623 raw reads from normal-virulence library and 35,291,803 raw reads from low-virulence library were obtained...
September 13, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27591791/marine-derived-compounds-as-binders-of-the-white-spot-syndrome-virus-vp28-envelope-protein-in-silico-insights-from-molecular-dynamics-and-binding-free-energy-calculations
#15
K C Sivakumar, T P Sajeevan, I S Bright Singh
White spot syndrome virus (WSSV) remains as one of the most dreadful pathogen of the shrimp aquaculture industry owing to its high virulence. The cumulative mortality reaches up to 100% within in 2-10days in a shrimp farm. Currently, no chemotherapeutics are available to control WSSV. The viral envelope protein, VP28, located on the surface of the virus particle acts as a vital virulence factor in the initial phases of inherent WSSV infection in shrimp. Hence, inhibition of envelope protein VP28 could be a novel way to deal with infection by inhibiting its interaction in the endocytic pathway...
August 28, 2016: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/27591045/comparative-microarray-profile-of-the-hepatopancreas-in-the-response-of-huanghai-no-2-fenneropenaeus-chinensis-to-white-spot-syndrome-virus
#16
Xiaoli Shi, Jie Kong, Xianhong Meng, Sheng Luan, Kun Luo, Baoxiang Cao, Ning Liu, Xia Lu, Kangyu Deng, Jiawang Cao, Yingxue Zhang, Hengheng Zhang, Xupeng Li
White spot syndrome virus (WSSV) infects all shrimp species and is the greatest detriment to shrimp culture. To better understand the mechanism of molecular responses to WSSV infection in "Huanghai No. 2" Fenneropenaeus chinensis, a microarray technique was used. Microarray gene expression profiling of 59,137 unigenes identified Differentially Expressed Genes (DEGs) both in live and moribund shrimp at early, peak and late phases. In live shrimp, 1307, 1479 and 1539 DEGs were obtained in the early, peak and late phase, respectively...
August 30, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27572278/crystal-structure-of-major-envelope-protein-vp24-from-white-spot-syndrome-virus
#17
Lifang Sun, Yintao Su, Yanhe Zhao, Zheng-Qing Fu, Yunkun Wu
White spot syndrome virus (WSSV) is one of the major and most serious pathogen in the shrimp industry. As one of the most abundant envelope protein, VP24 acts as a core protein interacting with other structure proteins and plays an important role in virus assembly and infection. Here, we have presented the crystal structure of VP24 from WSSV. In the structure, VP24 consists of a nine-stranded β-barrel fold with mostly antiparallel β-strands, and the loops extending out the β-barrel at both N-terminus and C-terminus, which is distinct to those of the other two major envelope proteins VP28 and VP26...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27544268/identification-of-an-anti-lipopolysacchride-factor-possessing-both-antiviral-and-antibacterial-activity-from-the-red-claw-crayfish-cherax-quadricarinatus
#18
Feng-Yu Lin, Yan Gao, Hao Wang, Qiu-Xia Zhang, Chang-Lin Zeng, Hai-Peng Liu
It is well-known that anti-lipopolysacchride factors (ALFs) are involved in the recognition and elimination of invading pathogens. In this study, the full-length ALF cDNA sequence of the red claw crayfish Cherax quadricarinatus (termed CqALF) was cloned from a suppression subtractive hybridization library constructed using red claw crayfish hematopoietic tissue cell (Hpt cell) cultures following challenge with white spot syndrome virus (WSSV). The full-length cDNA sequence of CqALF was 863 bp, and the open reading frame encoded 123 amino acids with a signal peptide in the N-terminus and a conserved LPS-binding domain...
October 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27542619/toll-receptor-response-to-white-spot-syndrome-virus-challenge-in-giant-freshwater-prawns-macrobrachium-rosenbergii
#19
Jinling Feng, Lingling Zhao, Min Jin, Tingting Li, Lei Wu, Yihong Chen, Qian Ren
Toll receptors are evolutionary ancient families of pattern recognition receptors with crucial roles in invertebrate innate immune response. In this study, we identified a Toll receptor (MrToll) from giant freshwater prawns (Macrobrachium rosenbergii). The full-length cDNA of MrToll is 4257 bp, which encodes a putative protein of 1367 amino acids. MrToll contains 17 LRR domains, a transmembrane domain, and a TIR domain. Phylogenetic analysis showed that MrToll was grouped with Drosophila Toll7 and other arthropod Tolls...
October 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27531577/pctoll3-was-involved-in-anti-vibrio-response-by-regulating-the-expression-of-antimicrobial-peptides-in-red-swamp-crayfish-procambarus-clarkii
#20
Jiang-Feng Lan, Shun Wei, Yu-Qing Wang, Yun-Jia Dai, Jia-Gang Tu, Li-Juan Zhao, Xin-Cang Li, Qi-Wei Qin, Nan Chen, Li Lin
Tolls and Toll-like receptors (TLRs) play an important role in host immune defenses by regulating the expression of antimicrobial peptides (AMPs) and cytokines, but the functional differences of crustacean Tolls from Drosophila Tolls or Mammal TLRs are largely unknown. A novel Toll receptor, named PcToll3, was identified from red swamp crayfish, Procambarus clarkii. It was widely expressed in all detected tissues, and its transcript in hemocytes was up-regulated at 12 h after Vibrio parahemolyticus (Vibrio) injection or at 24 h post white spot syndrome virus (WSSV) challenge...
October 2016: Fish & Shellfish Immunology
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