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Chuanqi Wang, Lingwei Ruan, Hong Shi, Xun Xu
The Wnt signaling mediated by Wnt proteins that orchestrate and influence a myriad of cellular processes, such as cell proliferation, differentiation, tumorigenesis, apoptosis, and participation in immune defense during microbe infection. Wnt5b is one of the Wnt signaling molecules that initiate the cascade. In this study, we cloned and characterized a Wnt5b homolog from Litopenaeus vannamei designed as LvWnt5b. The full length of LvWnt5b transcript was 1726 bp with an 1107 bp open reading frame that encoded a 368 aa protein, which contained 24 discontinuous and highly conserved cysteine...
January 8, 2018: Fish & Shellfish Immunology
Anees Fathima Noor, Tze Chiew Christie Soo, Farhana Mohd Ghani, Zee Hong Goh, Li Teng Khoo, Subha Bhassu
Background: Dystrophin, an essential protein functional in the maintenance of muscle structural integrity is known to be responsible for muscle deterioration during white spot syndrome virus (WSSV) infection among prawn species. Previous studies have shown the upregulation of dystrophin protein in Macrobrachium rosenbergii (the giant freshwater prawn) upon white spot syndrome virus (WSSV) infection. The literature has also suggested the important role of calcium ion alterations in causing such muscle diseases...
December 2017: Heliyon
Nittaya Bunsanong, Wilaiwan Chotigeat, Panchalika Deachamag, Suchera Thananimit
A Dot-blot immunogold assay (DBIA) was developed to detect white spot syndrome virus (WSSV) using the polyclonal antibody VP26 (anti-VP26). The anti-VP26 was immobilized on gold nanoparticles (Ab-AuNPs) and a nitrocellulose membrane was used as a detection pad. When the target WSSV bound to the Ab-AuNPs a reddish dots appeared on the surface of the membrane used within 2 to 5 minutes which could be seen with the naked eye. The test was able to detect WSSV at concentrations as low as 105 copies μl-1 of WSSV...
January 4, 2018: Biotechnology and Applied Biochemistry
K Saravanan, P Puneeth Kumar, J Praveenraj, Arunjyoti Baruah, T Sivaramakrishnan, T Sathish Kumar, S Pramod Kumar, R Kiruba Sankar, S Dam Roy
White spot syndrome virus (WSSV) is one of the most prevalent, widespread and devastating pathogen associated with shrimp population. The present study was aimed at screening the wild caught shrimps from Andaman and Nicobar Islands (ANI) for WSSV infection. Shrimp samples of different penaeid species including Penaeus monodon, Penaeus indicus, Penaeus merguiensis and Metapenaeus monoceros collected from nine different landing centers across the coast of ANI were screened for WSSV infection. Presence of white spots, a typical clinical sign of white spot disease was observed on the exoskeleton of WSSV infected shrimp samples...
December 2017: Virusdisease
Fredmoore L Orosco, Arturo O Lluisma
Marine animals typically harbor a community of viruses, a number of which are known to cause diseases. In shrimp aquaculture, viral pathogens are the principal causes of major economic losses. However, the composition of the viral load of shrimps in wild population is poorly known. In this study, we explored the viral diversity in the microbiome of wild Penaeus monodon collected from six sites in the Philippines, with a view to detecting pathogenic forms. We employed a metagenomic approach via particle-associated nucleic acid isolation, sequence-independent single primer amplification, and pyrosequencing...
September 2017: Virusdisease
Luzhi Jiang, Jinzhou Xiao, Liyuan Liu, Yingjie Pan, Shuling Yan, Yongjie Wang
White spot syndrome virus (WSSV) infection is commonly detected by vp28-qPCR assay in wild crayfish, Procambarus clarkii, a widespread crustacean species in the aquatic environment in China. The virions of crayfish WSSV have been isolated and purified. Based on TEM observation, they exhibited morphological structures that are identical to known WSSV. In addition, the WSSV major envelope protein VP28 was observed based on Western blot analysis of the total structural proteins of crayfish WSSV. PCR amplification and sequencing analyses of variable regions of ORF14/15, ORF23/24 and ORF94, along with viral genomic sequencing and phylogenomic analysis, indicated that the crayfish WSSV, named WSSV-CN-Pc, represents a new WSSV genotype...
September 2017: Virusdisease
Pattamaporn Kwankaew, Rachanida Praparatana, Phanthipha Runsaeng, Prapaporn Utarabhand
A diversity of C-type lectins (CTLs) was coming reported and they are known to participate in invertebrate innate immunity by act as pattern recognition receptor (PRR). In the present study, a unique CTL containing low-density lipoprotein receptor (LDLR) domain from Fenneropenaeus merguiensis (designated as FmLdlr) was cloned. Its sequence contained a single LDLR domain and one carbohydrate recognition domain (CRD) with a QAP motif putative for galactose-specific binding. The expression of FmLdlr was detected only in hemocytes of healthy shrimp...
December 28, 2017: Fish & Shellfish Immunology
Xiongchao Ma, Baozhen Sun, Fei Zhu
This study investigated the function of endonuclease-reverse transcriptase (mjERT) in Marsupenaeus japonicus. The 1129 bp cDNA sequence of mjERT was cloned from M. japonicus using rapid amplification of cDNA ends (RACE) PCR, and RT-qPCR analysis indicated that mjERT was highly expressed in the gills and hepatopancreas of M. japonicus. We also found that white spot syndrome virus (WSSV) or Vibrio alginolyticus challenge could enhance the expression of mjERT. When mjERT was inhibited, immune genes such as toll, p53, hemocyanin and tumor necrosis factor-α (TNF-α) were significantly down-regulated (P < ...
December 21, 2017: Fish & Shellfish Immunology
Feifei Yang, Shihao Li, Fuhua Li, Jianhai Xiang
White spot syndrome virus (WSSV) is a major viral pathogen in global shrimp farming, causing huge economic damage. Through penetrating the outer surface of the target tissues, WSSV enters into the cells of the target tissue to complete the replication process in the host. In the present study, a cuticle protein gene from Litopenaeus vannamei, designated as LvAMP13.4, was identified and proved to be involved in WSSV invasion. The deduced amino acid sequence of LvAMP13.4 contained a signal peptide and a conserved chitin-binding domain type 4 (ChBD4)...
December 21, 2017: Developmental and Comparative Immunology
Márcia Danielle A Dantas, Diego G Teixeira, Rita Cássia B Silva-Portela, Paulo Eduardo T Soares, João Paulo M S Lima, Lucymara F Agnez-Lima, Daniel Carlos F Lanza
White spot syndrome virus (WSSV) has been the cause of great economic losses in world shrimp farming. In this work the genome of a Brazilian WSSV isolate was determined from direct sequencing of total DNA extracted from an infected whiteleg shrimp, and assembled based on a chimera template approach. Comparisons between WSSV-BR and other isolates revealed that the Brazilian virus has a relatively small genome, and is very similar to isolates from Thailand and Mexico. A phylogenetic relationship using different approaches has demonstrated that these isolates share a common evolutionary history...
December 16, 2017: Virus Research
Suthinee Soponpong, Piti Amparyup, Anchalee Tassanakajon
DEAD (Asp-Glu-Ala-Asp)-box polypeptide 41 (DDX41), a receptor belonging to the DExD family, has recently been identified as an intracellular DNA sensor in vertebrates. Here, we report on the identification and functional characterization of PmDDX41, the first cytosolic DNA sensor in shrimp. By searching a Penaeus monodon expressed sequence tag (EST) database (, three cDNA fragments exhibiting similarity to DDX41 in various species were identified and assembled, resulting in a complete open reading frame of PmDDX41 that contains 1868-bp and encodes a putative protein of 620 amino acids...
December 14, 2017: Developmental and Comparative Immunology
Linwei Yang, Shengwen Niu, Jiefeng Gao, Hongliang Zuo, Jia Yuan, Shaoping Weng, Jianguo He, Xiaopeng Xu
The single whey acidic protein (WAP) domain (SWD)-containing proteins, also called type III crustins, are a group of antimicrobial peptides (AMPs) in crustaceans. At present, a number of SWDs have been identified in shrimp, which showed essential antibacterial activities. However, the roles of SWDs in antiviral immune responses have not been reported up to now. In this study, a novel SWD (LvSWD3) was identified from Pacific white shrimp, Litopenaeus vannamei, which contained a typical single WAP domain homologous to those of other crustacean SWDs...
December 13, 2017: Fish & Shellfish Immunology
Yalei Cui, Xiaoyuan Yang, Xiaobo Zhang
During host stress response against virus infection, some animal microRNAs (miRNAs) can be upregulated to restore the virus-caused metabolic disorder of host cells via suppressing the expressions of miRNAs' target genes. These antiviral miRNAs may have antitumor capacity, because tumorigenesis results from metabolic disorder of cells. However, this subject has not been explored. In this study, the results showed that shrimp miR-34, which was upregulated during white spot syndrome virus (WSSV) infection, had antiviral activity in shrimp...
December 15, 2017: Molecular Therapy. Nucleic Acids
Yaodong He, Tiantian Ma, Xiaobo Zhang
MicroRNAs (miRNAs), important factors in animal innate immunity, suppress the expressions of their target genes by binding to target mRNA's 3' untranslated regions (3'UTRs). However, the mechanism of synchronous regulation of multiple targets by a single miRNA remains unclear. In this study, the interaction between a white spot syndrome virus (WSSV) miRNA (WSSV-miR-N32) and its two viral targets (wsv459 and wsv322) was characterized in WSSV-infected shrimp. The outcomes indicated that WSSV-encoded miRNA (WSSV-miR-N32) significantly inhibited virus infection by simultaneously targeting wsv459 and wsv322...
2017: Frontiers in Immunology
Yali Han, Fang Li, Limei Xu, Feng Yang
White spot syndrome virus (WSSV) is a major pathogen of penaeid shrimp. Here we identified a new WSSV strain, WSSV-CN04, from naturally infected Marsupenaeus japonicus. Whole genomic sequencing results indicate that the WSSV-CN04 genome was 281 054 bp in length, and encoded 157 hypothetic proteins. The genome sequence of WSSV-CN04 was most closely related to the low-virulent strain WSSV-CN03, sharing 97.5% sequence identity. Notably, in WSSV-CN04, the major envelop protein VP24 was not only truncated but also absent in the virions...
December 11, 2017: Veterinary Research
Ming Li, Chunxia Ma, Haoyang Li, Jinxia Peng, Digang Zeng, Xiaohan Chen, Chaozheng Li
Antimicrobial peptides (AMPs) are the most important players in the innate immune system, providing a principal first-line of defense against the invading pathogens. Crustin, a type of whey acidic protein (WAP) domain-containing and cationic cysteine-rich AMP, can function in a protease inhibition or an effector molecule manner. In the present study, a new Crustin was cloned and identified from Pacific white shrimp Litopenaeus vannamei and designated as LvCrustinA. The full-length cDNA of LvCrustinA was 687 bp, with a 519 bp open reading frame (ORF) that encoded a peptide of 172 amino acids...
December 5, 2017: Fish & Shellfish Immunology
Benjaporn Phupet, Thanawat Pitakpornpreecha, Nuntaporn Baowubon, Phanthipha Runsaeng, Prapaporn Utarabhand
Lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) existed in diversity of invertebrates including shrimp plays a crucial role in an innate immunity via mediating the recognition of invading pathogens. In this study, LGBP was cloned and characterized from the hepatopancreas of Litopenaeus vannamei, named as LvLGBP. Its full-length cDNA of 1282 bp contained an open reading frame (1101 bp) encoding a peptide of 367 amino acids. The LGBP primary structure contained a glycosyl hydrolase domain, two integrin binding motifs, two kinase C phosphorylation sites, and two polysaccharide recognition motifs which were identified as a polysaccharide binding motif and a β-1,3-glucan recognition motif...
November 27, 2017: Developmental and Comparative Immunology
Xiaoxi Zhang, Xiaojun Zhang, Jianbo Yuan, Jiangli Du, Fuhua Li, Jianhai Xiang
Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs belong to the slow-type skeletal muscle actins, and 18 belong to the fast-type skeletal muscle actins; they were designated LvActinSSK and LvActinFSK, respectively...
November 30, 2017: Molecular Genetics and Genomics: MGG
Shihao Li, Zhiwei Wang, Fuhua Li, Kuijie Yu, Jianhai Xiang
Vascular endothelial growth factor (VEGF) signaling pathway is known to play key roles in endothelial cell proliferation, migration, angiogenesis, vascular permeability, inhibition of apoptosis, and virus infection. In the present study, a novel VEGFR gene (LvVEGFR2) was identified and characterized from Litopenaeus vannamei. The deduced amino acid sequence of LvVEGFR2 possessed typical features of VEGFRs reported in other species, including six IG-like domains, a transmembrane motif, a protein kinase (PK) domain, and one tyrosine-PK active site...
2017: Frontiers in Immunology
Hongliang Zuo, Jia Yuan, Shengwen Niu, Linwei Yang, Shaoping Weng, Jianguo He, Xiaopeng Xu
The molting-inhibiting hormones (MIHs) from the crustacean hyperglycemic hormone (CHH) family are a group of neuropeptides that are implicated in regulation of molting and reproduction in crustaceans. In this study, a novel protein containing a typical crustacean neuropeptide domain was identified from Litopenaeus vannamei. The protein showed high homology with other shrimp MIHs and was then designated as a MIH-like protein (MIHL). Among the detected tissues, the heart expressed the highest level of MIHL. The expression of MIHL could be significantly up-regulated after infection with white spot syndrome virus (WSSV), gram-negative bacterium Vibro parahaemolyticus and gram-positive bacterium Staphylococcus aureus, indicating that MIHL could be involved in immune responses...
November 17, 2017: Fish & Shellfish Immunology
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