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https://www.readbyqxmd.com/read/28587833/an-update-on-mechanism-of-entry-of-white-spot-syndrome-virus-into-shrimps
#1
Arunima Kumar Verma, Shipra Gupta, Shivesh Pratap Singh, Naresh Sahebrao Nagpure
Host-parasite relationships can be best understood at the level of protein-protein interaction between host and pathogen. Such interactions are instrumental in understanding the important stages of life cycle of pathogen such as adsorption of the pathogen on host surface followed by effective entry of pathogen into the host body, movement of the pathogen across the host cytoplasm to reach the host nucleus and replication of the pathogen within the host. White Spot Disease (WSD) is a havoc for shrimps and till date no effective treatment is available against the disease...
June 3, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28579079/ontogenetic-changes-in-the-expression-of-immune-related-genes-in-response-to-immunostimulants-and-resistance-against-white-spot-syndrome-virus-in-litopenaeus-vannamei
#2
T Rajkumar, G Taju, S Abdul Majeed, M Sinwan Sajid, S Santhosh Kumar, S Sivakumar, S Thamizhvanan, S Vimal, A S Sahul Hameed
In recent years, researchers have focused on viral and plant immunostimulants which could have beneficial effects in disease prevention and control in shrimp culture. At present, the application of the recombinant VP28 protein (r-VP28) and herbal immunostimulant has been considered as a more effective approach to prevent white spot syndrome (WSS) by enhancing the immune response in shrimp. In the present study, expression of selected immune related genes in response to r-VP28 and herbal immunostimulant mix (HIM) were separately studied qualitatively and quantitatively by RT-PCR and real time PCR, respectively during ontogenetic development from nauplius to juvenile stage in Litopenaeus vannamei...
June 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28564581/identification-characterization-and-function-analysis-of-the-nf-%C3%AE%C2%BAb-repressing-factor-nkrf-gene-from-litopenaeus-vannamei
#3
Wei Qiu, Jian-Hui He, Hongliang Zuo, Shengwen Niu, Chaozheng Li, Shuang Zhang, Shaoping Weng, Jianguo He, Xiaopeng Xu
The NF-κB family transcription factors regulate a wide spectrum of biological processes, in particular immune responses. The studies in human suggest that the NF-κB repressing factor (NKRF) negatively regulates the activity of NF-κB through a direct protein-protein interaction. However, the function of NKRF has not been studied outside mammals up to now. The current study identified a NKRF gene (LvNKRF) from the Pacific white shrimp, Litopenaeus vannamei, which showed homology with NKRFs from insects, fishes and mammals...
May 28, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28554838/the-identification-of-micrornas-involved-in-the-response-of-chinese-shrimp-fenneropenaeus-chinensis-to-white-spot-syndrome-virus-infection
#4
Xupeng Li, Xianhong Meng, Kun Luo, Sheng Luan, Xiaoli Shi, Baoxiang Cao, Jie Kong
MicroRNA (miRNA) is a class of small noncoding RNA, which is involved in the post-transcriptional regulation in all metazoan eukaryotes. MiRNAs might play an important role in the host response to virus infection. However, miRNAs in the aquatic crustacean species were not extensively investigated. To obtain a better understanding of the response of Chinese shrimp Fenneropenaeus chinensis to white spot syndrome virus (WSSV) infection, the sequence and expression profile of miRNAs in the hepatopancreas of WSSV-infected F...
May 26, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28554835/transcriptome-profiling-of-the-macrobrachium-rosenbergii-lymphoid-organ-under-the-white-spot-syndrome-virus-challenge
#5
Jun Cao, Lei Wu, Min Jin, Tingting Li, Kaimin Hui, Qian Ren
Macrobrachium rosenbergii is a crustacean with economic importance, and adult prawns are generally thought to be tolerant to white spot syndrome virus (WSSV) infection. Although certain genes are known to respond to WSSV infection and lymphoid tissue is an important immune organ, the response of lymphoid organ to WSSV infection is unclear. Next-generation sequencing was employed in this study to determine the transcriptome differences between WSSV infection and mock lymphoid organs. A total of 44,606,694 and 40,384,856 clean reads were generated and assembled into 73,658 and 72,374 unigenes from the control sample and the WSSV infection sample, respectively...
May 26, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28546026/the-crustin-like-peptide-plays-opposite-role-in-shrimp-immune-response-to-vibrio-alginolyticus-and-white-spot-syndrome-virus-wssv-infection
#6
Baozhen Sun, Zhi Wang, Fei Zhu
Crustin is an antimicrobial peptide (AMP) that plays a key role in innate immunity of crustaceans. In this study, we cloned the entire 660 bp crustin-like sequence with a 507 bp open reading frame encoding a 168 amino acid from Marsupenaeus japonicus. The crustin-like gene was primarily expressed in gills and over-expressed in shrimp hemocytes after challenge with WSSV or Vibrio alginolyticus. After knockdown crustin-like gene using specific double-stranded RNA (CRU-dsRNA), IMD, Rab7, L-lectin, mitogen-activated protein kinase, p53, prophenoloxidase and Rho were down-regulated and nitric oxide synthase, myosin and tumor necrosis factor-α were up-regulated in hemocytes at 24 h post dsRNA treatment...
May 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28545960/vaccination-with-multimeric-recombinant-vp28-induces-high-protection-against-white-spot-syndrome-virus-in-shrimp
#7
Suparat Taengchaiyaphum, Hideki Nakayama, Jiraporn Srisala, Ratny Khiev, Diva January Aldama-Cano, Siripong Thitamadee, Kallaya Sritunyalucksana
To improve the efficacy of WSSV protection, multimeric (tetrameric) recombinant VP28 (4XrVP28) was produced and tested in comparison with those of monomeric VP28 (1XrVP28). In vitro binding of either 1XrVP28 or 4XrVP28 to shrimp hemocyte surface was evident as early as 10 min after protein inoculation. Similar results were obtained in vivo when shrimp were injected with recombinant proteins that the proteins bound to the hemocyte surface could be detected since 5 min after injection. Comparison of the WSSV protection efficiencies of 1XrVP28 or 4XrVP28 were performed by injection the purified 1XrVP28 or 4XrVP28 (22...
May 22, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28539733/molecular-docking-of-phytoligands-to-the-viral-protein-receptor-p-monodon-rab7
#8
Jerrine Joseph, Raj Bhaskaran, Muthusamy Kaliraj, Muthiyah Muthuswamy, Arumugam Suresh
The development of shrimp aquaculture has been severely affected by viral diseases resulting in a huge economic burden to the industry. White spot disease (WSD) has caused severe mortality in farmed shrimp in many countries. Globally aquaculture industries face huge economic losses due to rapid spread of White Spot Syndrome Virus (WSSV) disease that can cause 100% mortality in a short period of 3-10 days. In the present study in order to prevent the spread of WSSV disease in shrimps, the receptor, PmRab7 has been chosen as the drug target...
2017: Bioinformation
https://www.readbyqxmd.com/read/28523918/current-status-of-viral-diseases-in-indian-shrimp-aquaculture
#9
G M Tandel, K Riji John, M Rosalind George, M J Prince Jeyaseelan
The intensification of aquaculture has been unique in showing the overwhelming changes in global food production in the last 100 years. Presently, it is playing a vital role in the economies of several countries. Conversely, it is also to be noted that the progression of aquaculture has been the foundation of anthropogenic alteration of a gigantic hierarchy and hence not astonishingly, it resulted in spread and emergence of an increasing group of new unknown diseases. In India, Penaeus monodon, black tiger shrimp was previously the foremost-cultivated shrimp species...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28523250/host-microrna-217-promotes-white-spot-syndrome-virus-infection-by-targeting-tube-in-the-chinese-mitten-crab-eriocheir-sinensis
#10
Ying Huang, Keke Han, Wen Wang, Qian Ren
MicroRNAs (miRNAs), a group of small molecule non-encoding RNAs, are key post-transcriptional regulators of gene expression that are implicated in many biological processes. In the current study, miR-217 from Eriocheir sinensis was selected for studying its roles during host-virus interaction. Overexpression or silencing of miR-217 led to considerable effects on white spot syndrome virus (WSSV) replication, implying that miR-217 played a positive role in WSSV infection. In insect High Five cells, miR-217 significantly inhibited Tube gene expression by binding to the 3'-untranslated region of the Tube...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28505172/increased-nucleoside-diphosphate-kinase-activity-induces-white-spot-syndrome-virus-infection-in-litopenaeus-vannamei
#11
Peng-Fei Liu, Qing-Hui Liu, Yin Wu, Jie Huang
Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP's) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection...
2017: PloS One
https://www.readbyqxmd.com/read/28496442/a-proteomic-study-of-hemocyte-proteins-from-mud-crab-scylla-paramamosain-infected-with-white-spot-syndrome-virus-or-vibrio-alginolyticus
#12
Baozhen Sun, Zhi Wang, Ziyan Wang, Xiongchao Ma, Fei Zhu
In this study, we investigated the hemocytes' immune response to white spot syndrome virus (WSSV) or Vibrio alginolyticus infection at the protein level. The differential proteomes from crab hemocytes infected with WSSV or V. alginolyticus were analyzed using the isobaric tags for relative and absolute quantitation approach immediately after infection. Using this approach, we identified 1,799 proteins by their by LC-MS/MS spectra and sequencing data. These included 157 upregulated proteins and 164 downregulated proteins after WSSV infection...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28484601/wsv023-interacted-with-litopenaeus-vannamei-%C3%AE-tubulin-complex-associated-proteins-2-and-decreased-the-formation-of-microtubules
#13
Yihong Chen, Haitao Bi, Xiaoyun Li, Zezhi Zhang, Haitao Yue, Shaoping Weng, Jianguo He
A previous study found the key transcription factor of Litopenaeus vannamei PERK-eIF2α pathway cyclic AMP-dependent transcription factor 4 (LvATF4) was involved in the transcriptional regulation of white spot syndrome virus (WSSV) gene wsv023. Knocked-down expression of LvATF4 reduced the viral copy number and the cumulative mortality of WSSV-infected shrimp. These results suggested that wsv023 may be critical to WSSV infection but the precise function of wsv023 was still unknown. By using co-immunoprecipitation and pull-down assays, we show that wsv023 interacts with L...
April 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28481405/hsp70-gene-polymorphisms-in-farmed-marine-shrimp-litopenaeus-vannamei-populations-exposed-to-white-spot-disease-and-infectious-myonecrosis
#14
A L Ferreira, R Maggioni, D Conceição, L M Perazzolo, R L Petersen
Single nucleotide polymorphisms (SNPs) are the best genetic markers for associative studies of the immune system in invertebrates. In the marine shrimp Litopenaeus vannamei, SNPs linked to disease resistance have been reported for some genes, such as hemocyanin, anti-lipopolysaccharide factor, and heat-shock protein 70 (Hsp70). In the present study, polymorphisms in the Hsp70 gene were investigated among three commercial L. vannamei populations bred in Northeast and South Brazil. The first population withstood a strong white spot disease outbreak; the second population suffered extended exposure to infectious myonecrosis; the third population was a high health population, which was experimentally infected with white spot syndrome virus (WSSV) in the present study...
May 4, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28479346/cqtoll-participates-in-antiviral-response-against-white-spot-syndrome-virus-via-induction-of-anti-lipopolysaccharide-factor-in-red-claw-crayfish-cherax-quadricarinatus
#15
Yan-Yao Li, Xiao-Xiao Chen, Feng-Yu Lin, Qiu-Fan Chen, Xing-Yuan Ma, Hai-Peng Liu
It is well known that Tolls/Toll like receptors (TLRs), a family of pattern recognition receptors, play important roles in immune responses. Previously, we found that a Toll transcript was increased in a transcriptome library of haematopoietic tissue (Hpt) cells from the red claw crayfish Cherax quadricarinatus post white spot syndrome virus infection. In the present study, a full-length cDNA sequence of Toll receptor (named as CqToll) was identified with 3482 bp which contained an open reading frame of 3021 bp encoding 1006 amino acids...
May 4, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28476665/identification-and-characterization-of-a-laccase-from-litopenaeus-vannamei-involved-in-anti-bacterial-host-defense
#16
Lili Shi, Siuming Chan, Chaozheng Li, Shuang Zhang
Phenoloxidases (POs) are a family of enzymes including tyrosinases, catecholases and laccases, which play an important role in immune defences of various invertebrates. Whether or not laccase exists in shrimp and its function is still poorly understood. In this study, a laccase (LvLac) was cloned and identified from Litopenaeus vannamei for the first time. The full length of LvLac is 3406 bp, including a 2034 bp open reading frame (ORF) coding for a putative protein of 677 amino acids with a signal peptide of 33 aa...
May 3, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28441623/identification-of-a-macrobrachium-nipponense-c-type-lectin-with-a-close-evolutionary-relationship-to-vertebrate-lectins
#17
Xin Huang, Tingting Li, Min Jin, Shaowu Yin, Wen Wang, Qian Ren
C-type lectins (CTLs) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. This study cloned and characterized a novel C-type lectin (MnCTL) of the oriental river prawn, Macrobrachium nipponense. The cloned MnCTL cDNA encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. The deduced MnCTL protein contains a single carbohydrate recognition domain (CRD) with an EPN (Glu-Pro-Asn) motif in calcium-binding site 2. Phylogenetic analysis indicated that MnCTL has a closer evolutionary relationship with vertebrate lectins than with invertebrate lectins...
July 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28432348/white-spot-syndrome-virus-vp28-specific-double-stranded-rna-provides-protection-through-a-highly-focused-sirna-population
#18
Pål Nilsen, Marius Karlsen, Kallaya Sritunyalucksana, Siripong Thitamadee
Several studies have demonstrated that injection of double-stranded RNAs (dsRNA) homologous to mRNA for the white spot syndrome virus (WSSV) viral protein 28 (VP28) can induce protection in shrimp against WSSV through RNA interference (RNAi). In comparison to shrimp injected with either PBS or a green fluorescent protein (GFP) nonspecific dsRNA, we obtained nearly complete protection against WSSV infection in shrimp injected with VP28 dsRNA. Upregulation of host genes associated with small RNA silencing was measured 48 hours post treatment in groups injected with dsRNA, and although the VP28-treated group remained moderately upregulated after challenge with WSSV, many-fold higher induction was observed in both control groups reflecting the ongoing viral infection...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28429823/changes-in-the-gut-microbiome-of-the-chinese-mitten-crab-eriocheir-sinensis-in-response-to-white-spot-syndrome-virus-wssv-infection
#19
Z F Ding, M J Cao, X S Zhu, G H Xu, R L Wang
Intestinal microorganisms play important roles in maintaining host health, but their functions in aquatic animal hosts have yet to be fully elucidated. The Chinese mitten crab, Eriocheir sinensis, is one such example. We attempted to identify the shift of gut microbiota that occurred in response to infection of white spot syndrome virus (WSSV), an emerging viral pathogen in the crab aquaculture industry. The microbiota may exert some control over aspects of the viral pathogenesis. We investigated the changes in composition and structure of the crab gut microbiome during various WSSV infection stages of 6 h post-infection (hpi) and 48 hpi, using a 16S rRNA approach on the MiSeq Illumina sequencing platform...
April 21, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28428061/identification-and-characterization-of-an-interleukin-16-like-gene-from-pacific-white-shrimp-litopenaeus-vannamei
#20
Qianhui Liang, Jiefu Zheng, Hongliang Zuo, Chaozheng Li, Shengwen Niu, Linwei Yang, Muting Yan, Shao-Ping Weng, Jianguo He, Xiaopeng Xu
Interleukins are a group of cytokines that play essential roles in immune regulation. Almost all interleukin genes are only found in vertebrates. In this study, an interleukin-16-like gene (LvIL-16L) was identified from Pacific white shrimp, Litopenaeus vannamei. LvIL-16L was predicted to encode a precursor (pro-LvIL-16L) with 1378 amino acids, sharing similarities with predicted pro-IL-16-like proteins from insects. The C-terminus of pro-LvIL-16L protein contained two PDZ domains homologous to the mature IL-16 cytokine of vertebrates...
April 17, 2017: Developmental and Comparative Immunology
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