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https://www.readbyqxmd.com/read/28709908/variable-rna-expression-from-recently-acquired-endogenous-viral-elements-eve-of-white-spot-syndrome-virus-wssv-in-shrimp
#1
Heny Budi Utari, Chumporn Soowannayan, Timothy W Flegel, Boonsirm Whityachumnarnkul, Maleeya Kruatrachue
The viral accommodation hypothesis proposes that endogenous viral elements (EVE) from both RNA and DNA viruses are being continually integrated into the shrimp genome by natural host processes and that they can result in tolerance to viral infection by fortuitous production of antisense, immunospecific RNA (imRNA). Thus, we hypothesized that previously reported microarray results for the presence of white spot syndrome virus (WSSV) open reading frames (ORFs) formerly called 151, 366 and 427 in a domesticated giant tiger shrimp (Penaeus monodon) breeding stock might have represented expression from EVE, since the stock had shown uninterrupted freedom from white spot disease (WSD) for many generations...
July 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28705723/a-pacifastacus-leniusculus-serine-protease-interacts-with-wssv
#2
Enen Guo, Gül Gizem Korkut, Phattarunda Jaree, Irene Söderhäll, Kenneth Söderhäll
Serine proteases are involved in many critical physiological processes including virus spread and replication. In the present study, we identified a new clip-domain serine protease (PlcSP) in the crayfish Pacifastacus leniusculus hemocytes, which can interact with the White Spot Syndrome Virus (WSSV) envelope protein VP28. It was characterized by a classic clip domain with six strictly conserved Cys residues, and contained the conserved His-Asp-Ser (H-D-S) motif in the catalytic domain. Furthermore, signal peptide prediction revealed that it has a 16-residue secretion signal peptide...
July 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28689772/characterization-and-function-of-a-%C3%AE-catenin-homolog-from-litopenaeus-vannamei-in-wssv-infection
#3
Jiazhen Sun, Lingwei Ruan, Hong Shi, Xun Xu
As a conserved signaling pathway, Wnt/β-catenin signaling pathway participates in many physiological activities, including cell differentiation, apoptosis and so on. β-catenin is the key molecule of Wnt/β-catenin signaling pathway and plays a pivotal role. In this article, a β-catenin homolog from Litopenaeus vannamei (designed as Lv-β-catenin) was cloned and its role in WSSV infection was investigated. Sequence analysis suggested that Lv-β-catenin had characters of β-catenin family. Semi-quantitative RT-PCR showed that Lv-β-catenin transcripted in all detected tissues...
July 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28686185/the-roles-of-%C3%AE-integrin-of-chinese-shrimp-fenneropenaeus-chinensis-in-wssv-infection
#4
Xiaoqian Tang, Fude Zhai, Xiuzhen Sheng, Jing Xing, Wenbin Zhan
Our previous study demonstrated that an integrin β subunit of Chinese shrimp (Fenneropenaeus chinensis) (FcβInt) plays an important role in white spot syndrome virus (WSSV) infection. In the present work, in order to further elucidate the potential role of FcβInt in WSSV infection, the recombinant extracellular domain of β integringene of F. Chinensis (rFcβInt-ER) was expressed in Escherichia coli BL21 (DE3), and the eukaryotic expression plasmid PcDNA3.1-FcβInt-ER (PFcβInt-ER) was also constructed. Far-western blotting was performed to determine the binding specificity of rFcβInt-ER to WSSV envelope proteins, and results showed that rFcβInt-ER was able to specifically interact with rVP31, rVP37, rVP110 and rVP187...
July 7, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28676694/resonant-dipolar-coupling-of-microwaves-with-confined-acoustic-vibrations-in-a-rod-shaped-virus
#5
Chi-Kuang Sun, Yi-Chun Tsai, Yi-Jan E Chen, Tzu-Ming Liu, Hui-Yuan Chen, Han-Ching Wang, Chu-Fang Lo
In this letter, we treat a rod-shaped virus as a free homogenous nanorod and identify its confined acoustic vibration modes that can cause strong resonant microwave absorption through electric dipolar excitation with a core-shell charge distribution. They are found to be the n = 4N-2 modes of the longitudinal modes of the nanorods, where N is an integer starting from 1 and n is the mode order quantum number. This study was confirmed by measuring the microwave absorption spectra of white spot syndrome virus (WSSV), which is a rod-shaped virus...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28648884/epigallocatechin-3-gallate-inhibit-replication-of-white-spot-syndrome-virus-in-scylla-paramamosain
#6
Zhi Wang, Baozhen Sun, Fei Zhu
Epigallocatechin-3-gallate (EGCG) has exhibited potential antibacterial and anticancer activities. In this study, we tested whether EGCG can protect the mud crab Scylla paramamosain against white spot syndrome virus (WSSV). Treatment with 1.5 mg/kg EGCG killed crab hemocytes but treatment with 1 mg/kg EGCG did not. Results of the virus challenge experiment confirmed that EGCG could enhance the survival rate of WSSV-challenged crabs, and treatment with 1 mg/kg EGCG significantly increased their resistance to WSSV compared with the control...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28647845/recent-progress-in-the-development-of-white-spot-syndrome-virus-vaccines-for-protecting-shrimp-against-viral-infection
#7
REVIEW
Shuying Feng, Chen Wang, Shu Hu, Qiongya Wu, Aifang Li
White spot syndrome virus (WSSV) has been extensively investigated since the white spot disease outbreak in shrimp in 1990. These investigations have mainly focused on determining the function of viral structural proteins, and on utilizing the envelope proteins as subunit vaccines to protect the host. These studies have shown that a WSSV vaccine can be a practical and effective approach for controlling WSSV infection. The development of such a vaccine has become an intense focus of research in the field. This research has resulted in significant progress in the development of WSSV vaccines...
June 24, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28587833/an-update-on-mechanism-of-entry-of-white-spot-syndrome-virus-into-shrimps
#8
Arunima Kumar Verma, Shipra Gupta, Shivesh Pratap Singh, Naresh Sahebrao Nagpure
Host-parasite relationships can be best understood at the level of protein-protein interaction between host and pathogen. Such interactions are instrumental in understanding the important stages of life cycle of pathogen such as adsorption of the pathogen on host surface followed by effective entry of pathogen into the host body, movement of the pathogen across the host cytoplasm to reach the host nucleus and replication of the pathogen within the host. White Spot Disease (WSD) is a havoc for shrimps and till date no effective treatment is available against the disease...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28579079/ontogenetic-changes-in-the-expression-of-immune-related-genes-in-response-to-immunostimulants-and-resistance-against-white-spot-syndrome-virus-in-litopenaeus-vannamei
#9
T Rajkumar, G Taju, S Abdul Majeed, M Sinwan Sajid, S Santhosh Kumar, S Sivakumar, S Thamizhvanan, S Vimal, A S Sahul Hameed
In recent years, researchers have focused on viral and plant immunostimulants which could have beneficial effects in disease prevention and control in shrimp culture. At present, the application of the recombinant VP28 protein (r-VP28) and herbal immunostimulant has been considered as a more effective approach to prevent white spot syndrome (WSS) by enhancing the immune response in shrimp. In the present study, expression of selected immune related genes in response to r-VP28 and herbal immunostimulant mix (HIM) were separately studied qualitatively and quantitatively by RT-PCR and real time PCR, respectively during ontogenetic development from nauplius to juvenile stage in Litopenaeus vannamei...
June 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28564581/identification-characterization-and-function-analysis-of-the-nf-%C3%AE%C2%BAb-repressing-factor-nkrf-gene-from-litopenaeus-vannamei
#10
Wei Qiu, Jian-Hui He, Hongliang Zuo, Shengwen Niu, Chaozheng Li, Shuang Zhang, Shaoping Weng, Jianguo He, Xiaopeng Xu
The NF-κB family transcription factors regulate a wide spectrum of biological processes, in particular immune responses. The studies in human suggest that the NF-κB repressing factor (NKRF) negatively regulates the activity of NF-κB through a direct protein-protein interaction. However, the function of NKRF has not been studied outside mammals up to now. The current study identified a NKRF gene (LvNKRF) from the Pacific white shrimp, Litopenaeus vannamei, which showed homology with NKRFs from insects, fishes and mammals...
May 28, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28554838/the-identification-of-micrornas-involved-in-the-response-of-chinese-shrimp-fenneropenaeus-chinensis-to-white-spot-syndrome-virus-infection
#11
Xupeng Li, Xianhong Meng, Kun Luo, Sheng Luan, Xiaoli Shi, Baoxiang Cao, Jie Kong
MicroRNA (miRNA) is a class of small noncoding RNA, which is involved in the post-transcriptional regulation in all metazoan eukaryotes. MiRNAs might play an important role in the host response to virus infection. However, miRNAs in the aquatic crustacean species were not extensively investigated. To obtain a better understanding of the response of Chinese shrimp Fenneropenaeus chinensis to white spot syndrome virus (WSSV) infection, the sequence and expression profile of miRNAs in the hepatopancreas of WSSV-infected F...
May 26, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28554835/transcriptome-profiling-of-the-macrobrachium-rosenbergii-lymphoid-organ-under-the-white-spot-syndrome-virus-challenge
#12
Jun Cao, Lei Wu, Min Jin, Tingting Li, Kaimin Hui, Qian Ren
Macrobrachium rosenbergii is a crustacean with economic importance, and adult prawns are generally thought to be tolerant to white spot syndrome virus (WSSV) infection. Although certain genes are known to respond to WSSV infection and lymphoid tissue is an important immune organ, the response of lymphoid organ to WSSV infection is unclear. Next-generation sequencing was employed in this study to determine the transcriptome differences between WSSV infection and mock lymphoid organs. A total of 44,606,694 and 40,384,856 clean reads were generated and assembled into 73,658 and 72,374 unigenes from the control sample and the WSSV infection sample, respectively...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28546026/the-crustin-like-peptide-plays-opposite-role-in-shrimp-immune-response-to-vibrio-alginolyticus-and-white-spot-syndrome-virus-wssv-infection
#13
Baozhen Sun, Zhi Wang, Fei Zhu
Crustin is an antimicrobial peptide (AMP) that plays a key role in innate immunity of crustaceans. In this study, we cloned the entire 660 bp crustin-like sequence with a 507 bp open reading frame encoding a 168 amino acid from Marsupenaeus japonicus. The crustin-like gene was primarily expressed in gills and over-expressed in shrimp hemocytes after challenge with WSSV or Vibrio alginolyticus. After knockdown crustin-like gene using specific double-stranded RNA (CRU-dsRNA), IMD, Rab7, L-lectin, mitogen-activated protein kinase, p53, prophenoloxidase and Rho were down-regulated and nitric oxide synthase, myosin and tumor necrosis factor-α were up-regulated in hemocytes at 24 h post dsRNA treatment...
July 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28545960/vaccination-with-multimeric-recombinant-vp28-induces-high-protection-against-white-spot-syndrome-virus-in-shrimp
#14
Suparat Taengchaiyaphum, Hideki Nakayama, Jiraporn Srisala, Ratny Khiev, Diva January Aldama-Cano, Siripong Thitamadee, Kallaya Sritunyalucksana
To improve the efficacy of WSSV protection, multimeric (tetrameric) recombinant VP28 (4XrVP28) was produced and tested in comparison with those of monomeric VP28 (1XrVP28). In vitro binding of either 1XrVP28 or 4XrVP28 to shrimp hemocyte surface was evident as early as 10 min after protein inoculation. Similar results were obtained in vivo when shrimp were injected with recombinant proteins that the proteins bound to the hemocyte surface could be detected since 5 min after injection. Comparison of the WSSV protection efficiencies of 1XrVP28 or 4XrVP28 were performed by injection the purified 1XrVP28 or 4XrVP28 (22...
May 22, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28539733/molecular-docking-of-phytoligands-to-the-viral-protein-receptor-p-monodon-rab7
#15
Jerrine Joseph, Raj Bhaskaran, Muthusamy Kaliraj, Muthiyah Muthuswamy, Arumugam Suresh
The development of shrimp aquaculture has been severely affected by viral diseases resulting in a huge economic burden to the industry. White spot disease (WSD) has caused severe mortality in farmed shrimp in many countries. Globally aquaculture industries face huge economic losses due to rapid spread of White Spot Syndrome Virus (WSSV) disease that can cause 100% mortality in a short period of 3-10 days. In the present study in order to prevent the spread of WSSV disease in shrimps, the receptor, PmRab7 has been chosen as the drug target...
2017: Bioinformation
https://www.readbyqxmd.com/read/28523918/current-status-of-viral-diseases-in-indian-shrimp-aquaculture
#16
G M Tandel, K Riji John, M Rosalind George, M J Prince Jeyaseelan
The intensification of aquaculture has been unique in showing the overwhelming changes in global food production in the last 100 years. Presently, it is playing a vital role in the economies of several countries. Conversely, it is also to be noted that the progression of aquaculture has been the foundation of anthropogenic alteration of a gigantic hierarchy and hence not astonishingly, it resulted in spread and emergence of an increasing group of new unknown diseases. In India, Penaeus monodon, black tiger shrimp was previously the foremost-cultivated shrimp species...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28523250/host-microrna-217-promotes-white-spot-syndrome-virus-infection-by-targeting-tube-in-the-chinese-mitten-crab-eriocheir-sinensis
#17
Ying Huang, Keke Han, Wen Wang, Qian Ren
MicroRNAs (miRNAs), a group of small molecule non-encoding RNAs, are key post-transcriptional regulators of gene expression that are implicated in many biological processes. In the current study, miR-217 from Eriocheir sinensis was selected for studying its roles during host-virus interaction. Overexpression or silencing of miR-217 led to considerable effects on white spot syndrome virus (WSSV) replication, implying that miR-217 played a positive role in WSSV infection. In insect High Five cells, miR-217 significantly inhibited Tube gene expression by binding to the 3'-untranslated region of the Tube...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28505172/increased-nucleoside-diphosphate-kinase-activity-induces-white-spot-syndrome-virus-infection-in-litopenaeus-vannamei
#18
Peng-Fei Liu, Qing-Hui Liu, Yin Wu, Jie Huang
Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP's) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection...
2017: PloS One
https://www.readbyqxmd.com/read/28496442/a-proteomic-study-of-hemocyte-proteins-from-mud-crab-scylla-paramamosain-infected-with-white-spot-syndrome-virus-or-vibrio-alginolyticus
#19
Baozhen Sun, Zhi Wang, Ziyan Wang, Xiongchao Ma, Fei Zhu
In this study, we investigated the hemocytes' immune response to white spot syndrome virus (WSSV) or Vibrio alginolyticus infection at the protein level. The differential proteomes from crab hemocytes infected with WSSV or V. alginolyticus were analyzed using the isobaric tags for relative and absolute quantitation approach immediately after infection. Using this approach, we identified 1,799 proteins by their by LC-MS/MS spectra and sequencing data. These included 157 upregulated proteins and 164 downregulated proteins after WSSV infection...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28484601/wsv023-interacted-with-litopenaeus-vannamei-%C3%AE-tubulin-complex-associated-proteins-2-and-decreased-the-formation-of-microtubules
#20
Yihong Chen, Haitao Bi, Xiaoyun Li, Zezhi Zhang, Haitao Yue, Shaoping Weng, Jianguo He
A previous study found the key transcription factor of Litopenaeus vannamei PERK-eIF2α pathway cyclic AMP-dependent transcription factor 4 (LvATF4) was involved in the transcriptional regulation of white spot syndrome virus (WSSV) gene wsv023. Knocked-down expression of LvATF4 reduced the viral copy number and the cumulative mortality of WSSV-infected shrimp. These results suggested that wsv023 may be critical to WSSV infection but the precise function of wsv023 was still unknown. By using co-immunoprecipitation and pull-down assays, we show that wsv023 interacts with L...
April 2017: Royal Society Open Science
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