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https://www.readbyqxmd.com/read/28231554/design-of-elution-strategy-for-simultaneous-detection-of-chloramphenicol-and-gentamicin-in-complex-samples-using-surface-plasmon-resonance
#1
Yinqiang Xia, Rongxin Su, Renliang Huang, Li Ding, Libing Wang, Wei Qi, Zhimin He
For the analysis of massive samples containing multiple analytes, the enhancement of detection efficiency is crucial. In this study, a facile method was developed for sequential detection of chloramphenicol (CAP) and gentamicin (GEN) in complex samples, e.g. milk, using a surface plasmon resonance (SPR)-based biosensor. Based on the immune inhibition format, two conjugates of antigen and bovine serum albumin (BSA)-denoted as CAP-BSA and GEN-BSA-were grafted on the same channel of the SPR sensor chip. Two standard curves for CAP and GEN were separately obtained by first mixing a single antibody with different concentrations of the relevant antigen...
February 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28231500/silver-nanoparticle-embedded-graphene-oxide-methotrexate-for-targeted-cancer-treatment
#2
Raj Kumar Thapa, Jae Hee Kim, Jee-Heon Jeong, Beom Soo Shin, Han-Gon Choi, Chul Soon Yong, Jong Oh Kim
Combination therapies are widely investigated cancer treatment modalities. Carbon based systems such as graphene oxide (GO), plasmonic nanoparticles such as silver nanoparticles (AgNPs), and the folate analog, methotrexate (MTX), have been separately studied for their potential anticancer effects. In this study, we combined these systems to develop AgNPs-embedded GO with conjugated MTX (MTX-GO/AgNPs) and studied their folate receptor-targeted anticancer effects. Results revealed successful formation of AgNPs on GO along with MTX conjugation as suggested by UV/visible, TEM, AFM, FTIR, and XRD analysis...
February 13, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28231288/characterization-and-expression-analysis-of-genes-encoding-ubiquitin-conjugating-domain-containing-enzymes-in-carica-papaya
#3
Dengwei Jue, Xuelian Sang, Bo Shu, Liqin Liu, Yicheng Wang, Zhiwei Jia, Yu Zou, Shengyou Shi
BACKGROUND: Ripening affects the quality and nutritional contents of fleshy fruits and is a crucial process of fruit development. Although several studies have suggested that ubiquitin-conjugating enzyme (E2s or UBC enzymes) are involved in the regulation of fruit ripening, little is known about the function of E2s in papaya (Carica papaya). METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we searched the papaya genome and identified 34 putative UBC genes, which were clustered into 17 phylogenetic subgroups...
2017: PloS One
https://www.readbyqxmd.com/read/28230997/universal-exciton-size-in-organic-polymers-is-determined-by-non-local-orbital-exchange-in-tddft
#4
Stefanie A Mewes, Felix Plasser, Andreas Dreuw
The exciton size of the lowest singlet excited state in a diverse set of organic π-conjugated polymers is studied and found to be a universal, system-independent quantity of approximately 7 Å in the single chain picture. With time-dependent density functional theory (TDDFT), its value as well as the overall description of the exciton is almost exclusively governed by the amount of non-local orbital exchange. This is traced back to the lack of the Coulomb attraction between the electron and hole quasiparticles in pure TDDFT, which is reintroduced only with the admixture of non-local orbital exchange...
February 23, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28230996/a-facile-n-mercaptoethoxyglycinamide-mega-linker-ap-proach-to-peptide-thioesterification-and-cyclization
#5
Patrick M M Shelton, Caroline E Weller, Champak Chatterjee
The C-terminal selective electrophilic activation of polypeptides is essential for site-specific peptide mod-ification and conjugation techniques such as Native Chemi-cal Ligation (NCL). Peptide C-terminal α-thioesters are par-ticularly valuable precursors for NCL, due to their hydrolytic stability in aqueous buffers and reactivity toward thiol nucle-ophiles. The synthesis of peptide α-thioesters, however, re-quires harsh acidic conditions or complex chemical manipu-lations, which ultimately limits their functional group com-patibility and broad utility...
February 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28230977/extracellular-self-assembly-of-functional-and-tunable-protein-conjugates-from-bacillus-subtilis
#6
Charlie Gilbert, Mark Howarth, Colin R Harwood, Tom Ellis
The ability to stably and specifically conjugate recombinant proteins to one another is a powerful approach for engineering multifunctional enzymes, protein therapeutics and novel biological materials. While many of these applications have been illustrated through in vitro and in vivo intracellular protein conjugation methods, extracellular self-assembly of protein conjugates offers unique advantages: simplifying purification, reducing toxicity and burden, and enabling tunability. Exploiting the recently described SpyTag-SpyCatcher system, we describe here how enzymes and structural proteins can be genetically-encoded to covalently conjugate in culture media following programmable secretion from Bacillus subtilis...
February 23, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28230474/unraveling-the-mode-of-action-of-new-promising-polymer-ruthenium-conjugates
#7
António Matos, Fernanda Marques, Rita Francisco, Tiago Moreira, Ana Preto, M Helena Garcia, Andreia Valente
No abstract text is available yet for this article.
January 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28230359/efficient-liver-targeting-by-polyvalent-display-of-a-compact-ligand-for-the-asialoglycoprotein-receptor
#8
Carlos A Sanhueza, Michael M Baksh, Benjamin Thuma, Marc D Roy, Sanjay Dutta, Cathy Préville, Boris A Chrunyk, Kevin Beaumont, Robert Dullea, Mark Ammirati, Shenping Liu, David Gebhard, James E Finley, Christopher T Salatto, Amanda King-Ahmad, Ingrid Stock, Karen Atkinson, Benjamin Reidich, Wen Lin, Rajesh Kumar, Meihua Tu, Elnaz Menhaji-Klotz, David A Price, Spiros Liras, M G Finn, Vincent Mascitti
A compact and stable bicyclic bridged ketal was developed as a ligand for the asialoglycoprotein receptor (ASGPR). This compound showed excellent ligand efficiency, and the molecular details of binding were revealed by the first X-ray crystal structures of ligand-bound ASGPR. This analogue was used to make potent di- and trivalent binders of ASGPR. Extensive characterization of the function of these compounds showed rapid ASGPR-dependent cellular uptake in vitro and high levels of liver/plasma selectivity in vivo...
February 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28230329/integrating-trans-abdominal-ultrasonography-with-fecal-steroid-metabolite-monitoring-to-accurately-diagnose-pregnancy-and-predict-the-timing-of-parturition-in-the-red-panda-ailurus-fulgens-styani
#9
Erin Curry, Lissa J Browning, Paul Reinhart, Terri L Roth
Red pandas (Ailurus fulgens styani) exhibit a variable gestation length and may experience a pseudopregnancy indistinguishable from true pregnancy; therefore, it is not possible to deduce an individual's true pregnancy status and parturition date based on breeding dates or fecal progesterone excretion patterns alone. The goal of this study was to evaluate the use of transabdominal ultrasonography for pregnancy diagnosis in red pandas. Two to three females were monitored over 4 consecutive years, generating a total of seven profiles (four pregnancies, two pseudopregnancies, and one lost pregnancy)...
February 23, 2017: Zoo Biology
https://www.readbyqxmd.com/read/28230204/high-resolution-imaging-of-living-mammalian-cells-bound-by-nanobeads-connected-antibodies-in-a-medium-using-scanning-electron-assisted-dielectric-microscopy
#10
Tomoko Okada, Toshihiko Ogura
Nanometre-scale-resolution imaging technologies for liquid-phase specimens are indispensable tools in various scientific fields. In biology, observing untreated living cells in a medium is essential for analysing cellular functions. However, nanoparticles that bind living cells in a medium are hard to detect directly using traditional optical or electron microscopy. Therefore, we previously developed a novel scanning electron-assisted dielectric microscope (SE-ADM) capable of nanoscale observations. This method enables observation of intact cells in aqueous conditions...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229983/establishment-and-application-of-early-risk-stratification-method-for-acute-abdominal-pain-in-adults
#11
Yu Wang, Hong Zhao, Zhen Zhou, Ci Tian, Hong-Li Xiao, Bao-En Wang
BACKGROUND: Acute abdominal pain is a common symptom of emergency patients. The severity was always evaluated based on physicians' clinical experience. The aim of this study was to establish an early risk stratification method (ERSM) for addressing adults with acute abdominal pain, which would guide physicians to take appropriate and timely measures following the established health-care policies. METHODS: In Cohort 1, the records of 490 patients with acute abdominal pain that developed within the past 72 h were enrolled in this study...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28229940/new-phenylpropanoid-and-6h-dibenzo-b-d-pyran-6-one-derivatives-from-the-stems-of-dasymaschalon-rostratum
#12
Zhang-Xin Yu, Zhi-Gang Niu, Xiao-Bao Li, Cai-Juan Zheng, Xin-Ming Song, Guang-Ying Chen, Xiao-Ping Song, Chang-Ri Han, Shu-Xian Wu
Three new phenylpropanoid derivatives, dasymaroacid A (1), dasymaroesters B and C (2 and 3), and one new polyoxygenated 6H-dibenzo[b,d]pyran-6-one derivative dasymarolactone D (4), together with seven known compounds (5-11), were isolated from the stems of Dasymaschalon rostratum Merr. Compounds 1 and 2 are unusual phenylpropanoid derivatives with a polymethyl substituted cyclopentene conjugated diketone as a substituent, and 3 is a unique cinnamic acid detective with a polymethyl substituted cyclohexene conjugated triketone as a substituent...
February 14, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28229598/dipicolylamine-functionalized-polyfluorene-based-gel-with-lower-critical-solution-temperature-preparation-characterization-and-application
#13
Chunlan Xiang, Hao Wan, Mingjing Zhu, Yijing Chen, Juan Peng, Gang Zhou
A thermoresponsive fluorescent polymer gel with lower critical solution temperature (LCST) phase transition has been prepared by cooperating conjugated fluorene homopolymer poly(2,7-(9,9-di(8-di(2-picolyl)aminooctyl))fluorene) (PPAOF) and small organic dye sulforhodamine B (SRB) or its sodium salt (SRB-Na). The sol-gel phase transition originates from the electrostatic interactions between the protonated pyridyl/amino groups in PPAOF and the sulfonic groups in the organic dye molecules, as revealed by FTIR, variable-temperature 1H NMR spectroscopies, and cyclic voltammetry measurements...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28229590/impacts-of-crosslinkers-on-biological-effects-of-mesoporous-silica-nanoparticles
#14
Yi-Ping Chen, Si-Han Wu, I-Chih Chen, Chien-Tsu Chen
Chemically synthesized crosslinkers play decisive roles in variable cargos attached to nanoparticles (NPs). Previous studies reported that surface properties, such as the size, charge, and surface chemistry, are particularly important determinants influencing the biological fate and actions of NPs and cells. Recent studies also focused on the relationship of serum proteins with the surface properties of NPs (also called the protein corona), which is recognized as a key factor in determining the cytotoxicity and biodistribution...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28228865/identification-of-beta-cell-dysfunction-at-the-pre-symptomatic-stage-of-diabetes-mellitus-by-novel-analytical-system-liquid-biopsy-measurements-in-femtograms
#15
REVIEW
Kurt Krapfenbauer
BACKGROUND: Diabetes mellitus is produced and progresses as a consequence of complex and gradual processes, in which a variety of alterations of the endocrine pancreas, are involved and which mainly result in beta cell failure. Those molecular alterations can be found in the bloodstream, which suggests that we could quantify specific biomarkers in plasma or serum by very sensitive methods before the onset diabetes mellitus is diagnosed. However, classical methods of protein analysis such as electrophoresis, Western blot, ELISA, and liquid chromatography are generally time-consuming, lab-intensive, and not sensitive enough to detect such alteration in a pre-symptomatic state of the disease...
December 2017: EPMA Journal
https://www.readbyqxmd.com/read/28228147/artificially-designed-recombinant-protein-composed-of-multiple-epitopes-of-foot-and-mouth-disease-virus-as-a-vaccine-candidate
#16
Ho-Bin Lee, Da-Chuan Piao, Jun-Yeong Lee, Jae-Yun Choi, Jin-Duck Bok, Chong-Su Cho, Sang-Kee Kang, Yun-Jaie Choi
BACKGROUND: Concerns regarding the safety of inactivated foot-and-mouth disease (FMD) vaccine have been raised since it is produced from cultured live FMD virus (FMDV). To overcome this issue, recombinant protein has been studied as an alternative vaccine. RESULTS AND CONCLUSION: We designed a chimerical multi-epitope recombinant protein (5BT), which is comprised of tandem repeats of five B cell epitopes (residue of VP1 136-162) derived from different FMDV variants and one T-cell epitope (residue of 3A 21-35)...
February 22, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28227976/user-intent-prediction-with-a-scaled-conjugate-gradient-trained-artificial-neural-network-for-lower-limb-amputees-using-a-powered-prosthesis
#17
Richard B Woodward, John A Spanias, Levi J Hargrove, Richard B Woodward, John A Spanias, Levi J Hargrove, John A Spanias, Richard B Woodward, Levi J Hargrove
Powered lower limb prostheses have the ability to provide greater mobility for amputee patients. Such prostheses often have pre-programmed modes which can allow activities such as climbing stairs and descending ramps, something which many amputees struggle with when using non-powered limbs. Previous literature has shown how pattern classification can allow seamless transitions between modes with a high accuracy and without any user interaction. Although accurate, training and testing each subject with their own dependent data is time consuming...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227196/low-cost-and-efficient-kurtosis-based-deflationary-ica-method-application-to-mrs-sources-separation-problem
#18
M Saleh, A Karfoul, A Kachenoura, L Senhadji, L Albera, M Saleh, A Karfoul, A Kachenoura, L Senhadji, L Albera, L Albera, L Senhadji, A Kachenoura, M Saleh, A Karfoul
Improving the execution time and the numerical complexity of the well-known kurtosis-based maximization method, the RobustICA, is investigated in this paper. A Newton-based scheme is proposed and compared to the conventional RobustICA method. A new implementation using the nonlinear Conjugate Gradient one is investigated also. Regarding the Newton approach, an exact computation of the Hessian of the considered cost function is provided. The proposed approaches and the considered implementations inherit the global plane search of the initial RobustICA method for which a better convergence speed for a given direction is still guaranteed...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226310/adhesion-of-ligand-conjugated-biosynthesized-magnetite-nanoparticles-to-triple-negative-breast-cancer-cells
#19
John D Obayemi, Jingjie Hu, Vanessa O Uzonwanne, Olushola S Odusanya, Karen Malatesta, Nicolas Anuku, Winston O Soboyejo
This paper presents the results of an experimental study of the adhesion forces between components of model conjugated magnetite nanoparticle systems for improved selectivity in the specific targeting of triple negative breast cancer. Adhesion forces between chemically synthesized magnetite nanoparticles (CMNPs), biosynthesized magnetite nanoparticles (BMNPs), as well as their conjugated systems and triple negative breast cancer cells (MDA-MB-231) or normal breast cells (MCF 10A) are elucidated at a nanoscale...
February 6, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28226265/alterations-of-ubiquitin-related-proteins-in-the-pathology-and-development-of-schizophrenia-evidence-from-human-and-animal-studies
#20
Jessica L Andrews, Frederic J Goodfellow, Natalie Matosin, Mollie K Snelling, Kelly A Newell, Xu-Feng Huang, Francesca Fernandez-Enright
Gene expression analyses in post-mortem schizophrenia brains suggest that a number of ubiquitin proteasome system (UPS) genes are associated with schizophrenia; however the status of UPS proteins in the schizophrenia brain is largely unknown. Ubiquitin related proteins are inherently involved in memory, neuronal survival and morphology, which are processes implicated in neurodevelopmental disorders such as schizophrenia. We examined levels of five UPS proteins (Protein Inhibitor of Activated STAT2 [PIAS2], F-Box and Leucine rich repeat protein 21 [FBXL21], Mouse Double Minute 2 homolog [MDM2], Ubiquitin Carboxyl-Terminal Hydrolase-L1 [UCHL1] and Ubiquitin Conjugating Enzyme E2D1 [UBE2D1]) involved in these neuronal processes, within the dorsolateral prefrontal cortex of post-mortem schizophrenia subjects and matched controls (n = 30/group), in addition to across neurodevelopmental time-points (juvenile, adolescent and adult stages of life), utilizing a well-established neurodevelopmental phencyclidine (PCP) animal model of schizophrenia...
January 18, 2017: Journal of Psychiatric Research
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