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https://www.readbyqxmd.com/read/28815745/recent-developments-in-open-tubular-capillary-electrochromatography-from-2016-2017
#1
REVIEW
Faustino M Tarongoy, Paul R Haddad, Joselito P Quirino
Interest in open-tubular capillary electrochromatography (OT-CEC) continues to thrive because of the inherent advantage of OT-CEC combining the high efficiency of capillary electrophoresis and the high selectivity of high performance liquid chromatography. For the period 2016 to 2017, novel materials have been developed as first-time stationary phases for OT-CEC and are grouped in this review as polymer-based materials, frameworks, nanoparticles, graphene-based materials, and biomaterials. Coating and fabrication methods mostly rely on covalent coating strategies while non-covalent immobilisation strategies like electrostatic assembly are notably still being employed...
August 16, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28814306/blood-acid-base-status-in-impala-aepyceros-melampus-immobilised-and-maintained-under-total-intravenous-anaesthesia-using-two-different-drug-protocols
#2
Gareth E Zeiler, Leith C R Meyer
BACKGROUND: In mammals, homeostasis and survival are dependent on effective trans-membrane movement of ions and enzyme function, which are labile to extreme acid-base changes, but operate efficiently within a narrow regulated pH range. Research in patients demonstrating a pH shifts outside the narrow regulated range decreased the cardiac output and systemic vascular resistance and altered the oxygen binding to haemoglobin. These cardiopulmonary observations may be applicable to the risks associated with anaesthesia and performance of wildlife ungulates on game farms...
August 16, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28810217/porous-cellulose-as-promoter-of-oil-production-by-the-oleaginous-yeast-lipomyces-starkeyi-using-mixed-agroindustrial-wastes
#3
Vassilios Ganatsios, Athanasios A Koutinas, Argyro Bekatorou, Vassilios Panagopoulos, Ibrahim M Banat, Antonia Terpou, Nikolaos Kopsahelis
Enhanced single cell oil (SCO) production by the oleaginous yeast Lipomyces starkeyi DSM 70296, immobilised on delignified porous cellulose, is reported. Pure glucose media were initially used. The effects of substrate pH and treatment temperature were evaluated, showing that 30°C and pH 5.0 were the optimum conditions for SCO production by the immobilised yeast. The immobilisation technique led to increased lipid accumulation and cell growth by 44% and 8%, respectively, in the glucose media, compared to free cells in suspension...
July 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28805084/covalent-immobilisation-of-transglutaminase-stability-and-applications-in-protein-pegylation
#4
Antonella Grigoletto, Anna Mero, Hiroki Yoshioka, Oddone Schiavon, Gianfranco Pasut
Microbial transglutaminase enzyme (mTGase) is an extremely useful enzyme that is increasingly employed in the food and pharmaceutical industries and as a tool for protein modification and tagging. The current study describes how we immobilised mTGase (iTGase) on a solid support to improve its stability during the PEGylation process by which polyethylene glycol chains are attached to protein and peptide drugs. When the enzyme was immobilised at the N-terminal sequence on agarose beads, it retained more than 53% of its starting activity...
August 13, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28803621/blunt-cervical-spine-injury-in-adult-polytrauma-incidence-injury-patterns-and-predictors-of-significant-ligament-injury-on-ct
#5
G Chilvers, U Janjua, S Choudhary
AIM: To describe the pattern of cervical spine fractures in adult major trauma and identify computed tomography (CT) parameters that can predict significant ligament injury when fractures are absent; to define the normal range for parameters used; and identify common variations due to position of cervical immobilisation in a trauma patient. MATERIALS AND METHODS: In this retrospective study all polytrauma patients imaged using multidetector CT over a period of 5 years were identified...
August 10, 2017: Clinical Radiology
https://www.readbyqxmd.com/read/28797246/characterisation-of-protein-families-in-spider-digestive-fluids-and-their-role-in-extra-oral-digestion
#6
André Walter, Jesper Bechsgaard, Carsten Scavenius, Thomas S Dyrlund, Kristian W Sanggaard, Jan J Enghild, Trine Bilde
BACKGROUND: Spiders are predaceous arthropods that are capable of subduing and consuming relatively large prey items compared to their own body size. For this purpose, spiders have evolved potent venoms to immobilise prey and digestive fluids that break down nutrients inside the prey's body by means of extra-oral digestion (EOD). Both secretions contain an array of active proteins, and an overlap of some components has been anecdotally reported, but not quantified. We systematically investigated the extent of such protein overlap...
August 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28795176/h2-driven-biocatalytic-hydrogenation-in-continuous-flow-using-enzyme-modified-carbon-nanotube-columns
#7
Ceren Zor, Holly A Reeve, Jonathan Quinson, Lisa A Thompson, Thomas H Lonsdale, Frank Dillon, Nicole Grobert, Kylie A Vincent
We describe the implementation of a system of immobilised enzymes for H2-driven NADH recycling coupled to a selective biotransformation to enable H2-driven biocatalysis in flow. This approach represents a platform that can be optimised for a wide range of hydrogenation steps and is shown here for enantioselective ketone reduction and reductive amination.
August 10, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28791907/a-one-step-procedure-for-immobilising-the-thermostable-carbonic-anhydrase-sspca-on-the-surface-membrane-of-escherichia-coli
#8
Sonia Del Prete, Rosa Perfetto, Mosè Rossi, Fatmah A S Alasmary, Sameh M Osman, Zeid AlOthman, Claudiu T Supuran, Clemente Capasso
The carbonic anhydrase superfamily (CA, EC 4.2.1.1) of metalloenzymes is present in all three domains of life (Eubacteria, Archaea, and Eukarya), being an interesting example of convergent/divergent evolution, with its seven families (α-, β-, γ-, δ-, ζ-, η-, and θ-CAs) described so far. CAs catalyse the simple, but physiologically crucial reaction of carbon dioxide hydration to bicarbonate and protons. Recently, our groups characterised the α-CA from the thermophilic bacterium, Sulfurihydrogenibium yellowstonense finding a very high catalytic activity for the CO2 hydration reaction (kcat = 9...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28780003/outcomes-of-gartland-type-iii-supracondylar-fractures-treated-using-blount-s-method
#9
C Muccioli, S ElBatti, I Oborocianu, O Rosello, F Solla, E Chau, J L Clement, V Rampal
BACKGROUND: Supracondylar fractures of the elbow with major displacement are usually treated by surgical pinning and less often non-operatively as described by Blount.The objective of this study was to assess the clinical and radiological outcomes of Gartland type III supracondylar fractures treated at least 3 years earlier using Blount's method. HYPOTHESIS: Blount's method produces good outcomes after more than 3 years when used to treat Gartland type III supracondylar fractures of the humerus...
August 2, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28774245/factors-influencing-outcome-in-the-management-of-scaphoid-non-union-and-comparison-of-matti-russe-and-interpositional-grafting-techniques
#10
Manish Kiran, Ian Persaud, Arpit Jariwala, Carlos Wigderowitz
BACKGROUND: Scaphoid non-union is often caused due to missed fractures, inadequate or inappropriate management. Matti-Russe and interpositional grafting techniques are used in the treatment of non-union. There are very few studies in literature that compare the outcomes of these techniques. The aim of our study was to analyse the factors influencing outcome in the management of scaphoid non-union and to compare the results of the Matti Russe procedure with interpositional grafting techniques...
September 2017: Journal of Hand Surgery Asian-Pacific Volume
https://www.readbyqxmd.com/read/28754750/postoperative-gluteal-compartment-syndrome-following-microsurgical-free-flap-hand-reconstruction-the-importance-of-early-recognition-and-treatment
#11
Umran Sarwar, Jason Ting
Compartment syndrome, a surgical emergency, is caused by an increase in pressure within a closed osseofascial space, often due to trauma. This causes a decrease in tissue perfusion and ultimately tissue necrosis and multiorgan failure if not treated in a timely fashion. Gluteal compartment syndrome is a rare variant and often caused by a period of immobilisation secondary to intoxication with alcohol or drugs or during long operations, typically in the supine position. We report on a case of gluteal compartment syndrome developing in a patient postoperatively following a long microsurgical procedure to a hand, which has not been documented before...
July 27, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28746949/-dislocation-of-the-pip-joint-treatment-of-a-common-ball-sports-injury
#12
Wibke Müller-Seubert, Gregor Bührer, Raymund E Horch
Background Fractures or fracture dislocations of the proximal interphalangeal joint often occur during sports or accidents. Dislocations of the PIP-joint are the most common ligamentary injuries of the hand. As this kind of injury is so frequent, hand surgeons and other physicians should be aware of the correct treatment. Objectives This paper summarises the most common injury patterns and the correct treatment of PIP-joint dislocations. Materials and Methods This paper reviews the current literature and describes the standardised treatment of PIP-joint dislocations...
July 26, 2017: Sportverletzung Sportschaden: Organ der Gesellschaft Für Orthopädisch-Traumatologische Sportmedizin
https://www.readbyqxmd.com/read/28745286/in-situ-green-synthesis-of-cu-nanoparticles-supported-on-natural-natrolite-zeolite-for-the-reduction-of-4-nitrophenol-congo-red-and-methylene-blue
#13
Mahmoud Nasrollahzadeh, S Mohammad Sajadi, Mehdi Maham, Hamid Reza Dasmeh
This study demonstrates a clean, non-toxic and environment friendly synthetic strategy for the preparation of the Natrolite zeolite/Cu nanoparticles (NPs) using Natrolite zeolite as a natural support and Anthemis xylopoda flowers aqueous extract as a reducing and stabilising agent for the synthesis of Cu NPs. Cu NPs with 20 nm diameter were immobilised homogeneously on the surface of Natrolite zeolite. The synthesised Natrolite zeolite/Cu NPs was used as an environmentally benign catalyst for the reduction of 4-nitrophenol, congo red and methylene blue in aqueous media at an ambient temperature...
August 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28738644/biosensor-for-the-characterisation-of-hmao-b-inhibitors-and-the-quantification-of-selegiline
#14
Maximilian Aigner, Patricia Preissegger, Kurt Kalcher, Eda Mehmeti, Peter Macheroux, Dale Edmondson, Astrid Ortner
A novel human monoamine oxidase B (hMAO B) based biosensor for inhibitory measurements was developed. It allows both the characterisation of the type of enzyme inhibition and the sensitive and simple determination of inhibitors like selegiline hydrochloride. The sensor consists of a screen printed carbon working electrode modified with 20% manganese dioxide (MnO2) and the enzyme hMAO B, which was immobilised on the electrode via a dialysis membrane (regenerated cellulose, molecular weight cut-off 14000). Inhibition of hMAO B is evaluated by adding different concentrations of the inhibitor selegiline hydrochloride to the enzyme and applying a defined amount of the hMAO B substrate phenylethylamine (PEA)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28721003/patient-compliance-with-venous-thromboembolism-prophylaxis-vte
#15
John Ross Blackwell, Parag Raval, John-Patrick Quigley, Amit Patel, Donald McBride
Venous thromboembolic disease (VTE) comprises pulmonary embolism (PE) and deep vein thrombosis (DVT), and causes morbidity and mortality, particularly in trauma and orthopaedic patients. Prevalence of 0.9% and 1.2% respectively are reported, with mortality rates up to 13.8%. Chemical thromboprophylactic agents including low molecular weight heparin (LMWH) are considered cost effective in reducing VTE risk. Evidence for anti-platelets including Aspirin for VTE prophylaxis is less compelling and is not supported as monotherapy...
April 2017: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/28718841/development-of-an-immunosensor-for-pfhrp-2-as-a-biomarker-for-malaria-detection
#16
Aver Hemben, Jon Ashley, Ibtisam E Tothill
Plasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent's suitability for the sensor's development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and characterised and then used to develop the immunosensor for PfHRP 2, which enables a low cost, easy to use, and sensitive biosensor for malaria diagnosis...
July 18, 2017: Biosensors
https://www.readbyqxmd.com/read/28717615/outcomes-of-prolotherapy-for-intra-tendinous-achilles-tears-a-case-series
#17
Otto Chan, Ben Havard, Sarah Morton, Mel Pritchard, Nicola Maffulli, Tom Crisp, Nat Padhiar, Jeremy David Perry, John King, Dylan Morrissey
BACKGROUND: The intra-tendinous tear is a new pathology that is defined as a discontinuity of fibres situated entirely within the tendon. Prolotherapy involves injecting an irritant, such as hyperosmolar dextrose, to stimulate a tissue healing response and ultimately reduce pain. METHODS: 43 consecutive patients diagnosed with an intra-tendinous tear were included (27 males: 16 females, mean (SD) age 41 (11.3). Patients were injected with 0.4ml-1.5ml (mean 0.8ml) of 50% dextrose and 0...
January 2017: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/28714671/facile-method-for-the-preparation-of-covalent-triazine-framework-coated-monoliths-as-catalyst-support-applications-in-c1-catalysis
#18
Anastasiya V Bavykina, Alma I Olivos-Suarez, Dmitrii Osadchii, Rahul Valecha, Robert Franz, Michiel Makkee, Freek Kapteijn, Jorge Gascon
A quasi chemical vapor deposition method for the manufacture of well-defined covalent triazine framework (CTF) coatings on cordierite monoliths is reported. The resulting supported porous organic polymer is an excellent support for the immobilization of two different homogeneous catalysts: (1) an Ir(III)Cp*-based catalyst for the hydrogen production from formic acid and (2) a Pt(II)-based catalyst for the direct activation of methane via Periana chemistry. The immobilized catalysts display a much higher activity in comparison with the unsupported CTF operated in slurry because of improved mass transport...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28711029/an-oxygen-independent-and-membrane-less-glucose-biobattery-supercapacitor-hybrid-device
#19
Xinxin Xiao, Peter Ó Conghaile, Dónal Leech, Roland Ludwig, Edmond Magner
Enzymatic biofuel cells can generate electricity directly from the chemical energy of biofuels in physiological fluids, but their power density is significantly limited by the performance of the cathode which is based on oxygen reduction for in vivo applications. An oxygen-independent and membrane-less glucose biobattery was prepared that consists of a dealloyed nanoporous gold (NPG) supported glucose dehydrogenase (GDH) bioanode, immobilised with the assistance of conductive polymer/Os redox polymer composites, and a solid-state NPG/MnO2 cathode...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28706267/a-simple-add-and-display-method-for-immobilisation-of-cancer-drug-on-his-tagged-virus-like-nanoparticles-for-controlled-drug-delivery
#20
Roya Biabanikhankahdani, Saadi Bayat, Kok Lian Ho, Noorjahan Banu Mohamed Alitheen, Wen Siang Tan
pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-responsive nanojoints which released DOX from VLNPs in a controlled manner. The His-tagged VLNPs conjugated non-covalently with NTA-DOX, and cross-linked with folic acid (FA) were able to specifically target and deliver the DOX into ovarian cancer cells via folate receptor (FR)-mediated endocytosis...
July 13, 2017: Scientific Reports
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