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https://www.readbyqxmd.com/read/28230769/hypoallergenic-variant-of-the-major-egg-white-allergen-gal-d-1-produced-by-disruption-of-cysteine-bridges
#1
Pathum Dhanapala, Dulashi Withanage-Dona, Mimi L K Tang, Tim Doran, Cenk Suphioglu
Gal d 1 (ovomucoid) is the dominant allergen in the chicken egg white. Hypoallergenic variants of this allergen can be used in immunotherapy as an egg allergy treatment approach. We hypothesised that disruption of two of the nine cysteine-cysteine bridges by site-directed mutagenesis will allow the production of a hypoallergenic variant of the protein; Methods: Two cysteine residues at C192 and C210 in domain III of the protein were mutated to alanine using site-directed mutagenesis, to disrupt two separate cysteine-cysteine bridges...
February 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28217778/a-critical-insight-into-the-development-pipeline-of-microfluidic-immunoassay-devices-for-the-sensitive-quantitation-of-protein-biomarkers-at-the-point-of-care
#2
REVIEW
Ana I Barbosa, Nuno M Reis
The latest clinical procedures for the timely and cost-effective diagnosis of chronic and acute clinical conditions, such as cardiovascular diseases, cancer, chronic respiratory diseases, diabetes or sepsis (i.e. the biggest causes of death worldwide), involve the quantitation of specific protein biomarkers released into the blood stream or other physiological fluids (e.g. urine or saliva). The clinical thresholds are usually in the femtomolar to picolomar range, and consequently the measurement of these protein biomarkers heavily relies on highly sophisticated, bulky and automated equipment in centralised pathology laboratories...
February 20, 2017: Analyst
https://www.readbyqxmd.com/read/28214747/immunorecognition-magnetic-supports-for-the-development-of-an-electrochemical-immunoassay-for-azaspiracid-detection-in-mussels
#3
Sandra Leonardo, Maria Rambla-Alegre, Ingunn A Samdal, Christopher O Miles, Jane Kilcoyne, Jorge Diogène, Ciara K O'Sullivan, Mònica Campàs
As azaspiracids (AZAs) are being reported from the coastal waters of an increasing number of countries on a global scale, the need for rapid, simple and cost-effective methods to detect these marine toxins and protect seafood consumers' health is becoming evident. A magnetic bead (MB)-based direct immunoassay for the detection of AZAs, using protein G-coated MBs as supports for antibody immobilisation and peroxidase-labelled AZA as a tracer is detailed. A colorimetric approach was first developed to optimise the experimental parameters and establish the cross-reactivity factors for AZA-1-10...
February 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28213867/re-establishment-of-rigor-mortis-evidence-for-a-considerably-longer-post-mortem-time-span
#4
Chiara Crostack, Susanne Sehner, Tobias Raupach, Sven Anders
Re-establishment of rigor mortis following mechanical loosening is used as part of the complex method for the forensic estimation of the time since death in human bodies and has formerly been reported to occur up to 8-12 h post-mortem (hpm). We recently described our observation of the phenomenon in up to 19 hpm in cases with in-hospital death. Due to the case selection (preceding illness, immobilisation), transfer of these results to forensic cases might be limited. We therefore examined 67 out-of-hospital cases of sudden death with known time points of death...
February 17, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28213234/dna-wrapped-multi-walled-carbon-nanotube-modified-electrochemical-biosensor-for-the-detection-of-escherichia-coli-from-real-samples
#5
Dilsat Ozkan-Ariksoysal, Yasin Ugur Kayran, Fethiye Ferda Yilmaz, Anton Alexandru Ciucu, Iulia Gabriela David, Vasile David, Mine Hosgor-Limoncu, Mehmet Ozsoz
This paper introduces DNA-wrapped multi-walled carbon nanotube (MWCNT)-modified genosensor for the detection of Escherichia coli (E. coli) from polymerase chain reaction (PCR)-amplified real samples while Staphylococcus aureus (S. aureus) was used to investigate the selectivity of the biosensor. The capture probe specifically recognizing E. coli DNA and it was firstly interacted with MWCNTs for wrapping of single-stranded DNA (ssDNA) onto the nanomaterial. DNA-wrapped MWCNTs were then immobilised on the surface of disposable pencil graphite electrode (PGE) for the detection of DNA hybridization...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28212869/improvement-of-simultaneous-cr-vi-and-phenol-removal-by-an-immobilised-bacterial-consortium-and-characterisation-of-biodegradation-products
#6
Ornella M Ontañon, Paola S González, Germán Barros, Elizabeth Agostini
Microbial bioremediation emerged some decades ago as an eco-friendly technology to restore polluted sites. Traditionally, the search for microorganisms suitable for bioremediation has been based on the selection of isolated strains able to remove a specific type of pollutant. However, this strategy has now become obsolete, since co-pollution is a global reality. Thus, current studies attempt to find bacterial cultures capable of coping with a mixture of organic and inorganic compounds. In this sense, the bacterial consortium SFC 500-1 has demonstrated efficiency for Cr(VI) and phenol removal, both of which are found in many industrial wastewaters...
February 14, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28205478/scaphotrapeziotrapezoidal-joint-osteoarthritis-a-systematic-review-of-surgical-treatment
#7
Victoria M Deans, Zafar Naqui, Lindsay T S W Muir
BACKGROUND: The management of scaphotrapeziotrapezoidal (STT) joint osteoarthritis (OA) remains controversial. This systematic review aims to review the evidence for surgical interventions specific to STT OA. METHODS: Medline and Embase libraries were searched using a pre-defined search strategy in October 2014. All study designs and languages were included and evaluated by two reviewers (VMD and LM) against the inclusion/exclusion criteria. The study eligibility criteria included papers discussing surgical treatment of STT OA, and the review was conducted using the PRISMA guidelines...
March 2017: Journal of Hand Surgery Asian-Pacific Volume
https://www.readbyqxmd.com/read/28199953/evaluation-of-single-and-stack-membraneless-enzymatic-fuel-cells-based-on-ethanol-in-simulated-body-fluids
#8
J Galindo-de-la-Rosa, N Arjona, A Moreno-Zuria, E Ortiz-Ortega, M Guerra-Balcázar, J Ledesma-García, L G Arriaga
The purpose of this work is to evaluate single and double-cell membraneless microfluidic fuel cells (MMFCs) that operate in the presence of simulated body fluids SBF, human serum and blood enriched with ethanol as fuels. The study was performed using the alcohol dehydrogenase enzyme immobilised by covalent binding through an array composed of carbon Toray paper as support and a layer of poly(methylene blue)/tetrabutylammonium bromide/Nafion and glutaraldehyde (3D bioanode electrode). The single MMFC was tested in a hybrid microfluidic fuel cell using Pt/C as the cathode...
February 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28199927/benzimidazole-acrylonitriles-as-multifunctional-push-pull-chromophores-spectral-characterisation-protonation-equilibria-and-nanoaggregation-in-aqueous-solutions
#9
Ema Horak, Robert Vianello, Marijana Hranjec, Svjetlana Krištafor, Grace Karminski Zamola, Ivana Murković Steinberg
Heterocyclic donor-π-acceptor molecular systems based on an N,N-dimethylamino phenylacrylonitrile benzimidazole skeleton have been characterised and are proposed for potential use in sensing applications. The benzimidazole moiety introduces a broad spectrum of useful multifunctional properties to the system including electron accepting ability, pH sensitivity and compatibility with biomolecules. The photophysical characterisation of the prototropic forms of these chromophores has been carried out in both solution and on immobilisation in polymer films...
February 7, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28199055/photo-induced-click-chemistry-for-dna-surface-structuring-via-direct-laser-writing
#10
Ljiljana Fruk, Antonina Kerbs, Patrick Mueller, Michael Kaupp, Ishtiaq Ahmed, Alexander S Quick, Doris Abt, Martin Wegener, Christof M Niemeyer, Christopher Barner-Kowollik
Oligonucleotides containing photo-caged dienes were prepared and shown to react quantitatively in a light-induced Diels-Alder cycloaddition with functional maleimides in aqueous solution within minutes. Due to its high yield and fast rate, the reaction was exploited for DNA surface patterning with sub-micrometer resolution employing direct laser writing (DLW). Functional DNA arrays were written via direct laser writing (DLW) in variable patterns, which were further encoded with fluorophores and proteins through DNA directed immobilisation...
February 15, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28194924/constructing-definitions-of-safety-risks-while-nurses-care-for-hospitalised-older-people-secondary-analysis-of-qualitative-data
#11
Sherry Dahlke, Wendy A Hall, Jennifer Baumbusch
AIM: The aim of this secondary qualitative descriptive analysis was to examine how nurses construct a definition of older peoples' safety risks and provide care while working within organisational contexts that are focused on diminishing patient risks. BACKGROUND: Numbers of older patients are increasing in acute hospital contexts-contexts that place their focus on patient safety. Nurses need to manage tensions between older peoples' risks, evidence-informed practice decisions, limited resources and organisational emphases on patient falls...
February 14, 2017: International Journal of Older People Nursing
https://www.readbyqxmd.com/read/28192970/antibody-mimetics-for-the-detection-of-small-organic-compounds-using-a-quartz-crystal-microbalance
#12
Eleni Koutsoumpeli, Christian Tiede, James Murray, Anna Ah-San Tang, Robin Stefan Bon, Darren Tomlinson, Steven D Johnson
Conventional immunoassays rely on antibodies that provide high affinity, specificity and selectivity against a target analyte. However, the use of antibodies for the detection of small-sized, non-immunogenic targets, such as pharmaceuticals and environmental contaminants presents a number of challenges. Recent advances in protein engineering have led to the emergence of antibody mimetics that offer the high affinity and specificity associated with antibodies but with reduced batch-to-batch variability, high stability and in vitro selection to ensure rapid discovery of binders against a wide range of targets...
February 1, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28189099/miniaturising-acute-toxicity-and-feeding-rate-measurements-in-daphnia-magna
#13
Konstantinos Grintzalis, Wenkui Dai, Konstantinos Panagiotidis, Alexia Belavgeni, Mark R Viant
Phenotypic markers of animal health form an essential component of regulatory toxicology. Immobilisation of neonate water fleas - Daphnia magna - as a surrogate measure of their mortality following exposure to a chemical for 24-48h forms the basis of the internationally utilised OECD acute toxicity test 202. A second important marker of animal physiology and health is feeding rate, which in Daphnia is determined by measuring the algae feeding rate. Given the widespread use of OECD test 202 for acute toxicity as well as the quantification of feeding rate in toxicological studies of daphniids, significant benefits could result from miniaturising this assay...
February 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28188816/a-chemical-genomics-approach-to-drug-reprofiling-in-oncology-antipsychotic-drug-risperidone-as-a-potential-adenocarcinoma-treatment
#14
Suzanne J Dilly, Andrew J Clark, Andrew Marsh, Daniel A Mitchell, Ricky Cain, Colin W G Fishwick, Paul C Taylor
Drug reprofiling is emerging as an effective paradigm for discovery of cancer treatments. Herein, an antipsychotic drug is immobilised using the Magic Tag(®) chemical genomics tool and screened against a T7 bacteriophage displayed library of polypeptides from Drosophila melanogaster, as a whole genome model, to uncover an interaction with a section of 17-β-HSD10, a proposed prostate cancer target. A computational study and enzyme inhibition assay with full length human 17-β-HSD10 identifies risperidone as a drug reprofiling candidate...
February 8, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28188567/breast-cancer-patients-perceptions-of-a-virtual-learning-environment-for-pretreatment-education
#15
Yobelli Alexandra Jimenez, Wei Wang, Kirsty Stuart, Steven Cumming, David Thwaites, Sarah Lewis
The process and technicalities of radiation therapy (RT) for cancer treatment can be challenging for patients to understand as RT involves complex procedures, highly specialised equipment, and radiation itself has limited sensory characteristics. Hence, it is imperative that education programs are specifically planned and developed to suit the needs of patients, address radiation as an entity and include salient visual aids. In this context, the Virtual Environment for Radiotherapy Training (VERT) system, primarily created for RT practitioner simulation, may provide unique opportunities for patient education...
February 10, 2017: Journal of Cancer Education: the Official Journal of the American Association for Cancer Education
https://www.readbyqxmd.com/read/28184244/solid-binding-peptides-for-immobilisation-of-thermostable-enzymes-to-hydrolyse-biomass-polysaccharides
#16
Andrew Care, Kerstin Petroll, Emily S Y Gibson, Peter L Bergquist, Anwar Sunna
BACKGROUND: Solid-binding peptides (SBPs) bind strongly to a diverse range of solid materials without the need for any chemical reactions. They have been used mainly for the functionalisation of nanomaterials but little is known about their use for the immobilisation of thermostable enzymes and their feasibility in industrial-scale biocatalysis. RESULTS: A silica-binding SBP sequence was fused genetically to three thermostable hemicellulases. The resulting enzymes were active after fusion and exhibited identical pH and temperature optima but differing thermostabilities when compared to their corresponding unmodified enzymes...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28182663/dihydrobenz-e-1-4-oxazepin-2-3h-ones-a-new-anthelmintic-chemotype-immobilising-whipworm-and-reducing-infectivity-in-vivo
#17
Frederick A Partridge, Emma A Murphy, Nicky J Willis, Carole J R Bataille, Ruth Forman, Narinder Heyer-Chauhan, Bruno Marinič, Daniel J C Sowood, Graham M Wynne, Kathryn J Else, Angela J Russell, David B Sattelle
Trichuris trichiura is a human parasitic whipworm infecting around 500 million people globally, damaging the physical growth and educational performance of those infected. Current drug treatment options are limited and lack efficacy against the worm, preventing an eradication programme. It is therefore important to develop new treatments for trichuriasis. Using Trichuris muris, an established model for T. trichiura, we screened a library of 480 novel drug-like small molecules for compounds causing paralysis of the ex vivo adult parasite...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28169039/the-definite-risks-and-questionable-benefits-of-liberal-pre-hospital-spinal-immobilisation
#18
Thomas Adam Purvis, Brian Carlin, Peter Driscoll
INTRODUCTION: The routine practice of pre-hospital spinal immobilisation (phSI) for patients with suspected spinal injury has existed for decades. However, the controversy surrounding it resulted in the 2013 publication of a Consensus document by the Faculty of Pre-Hospital Care. The question remains as to whether the quality of evidence in the literature is sufficient to support the Consensus guidelines. This critical review aims to determine the validity of current recommendations by balancing the potential benefits and side effects of phSI...
January 26, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28167427/feasibility-of-using-demolition-waste-as-an-alternative-heavy-metal-immobilising-agent
#19
Seelawut Damrongsiri
Demolition waste consisting of cement paste, lightweight concrete and bricks is a worthless material generated by a growing city. However, research suggests that it may be applied as an alternative heavy metal immobilising agent. The diverse characteristics of demolition waste were examined. Cadmium was selected as there presentative heavy metal to investigate demolition waste adsorption capacity. The solid-liquid distribution coefficients (Kd) were observed. The adsorption isotherms were applied to investigate adsorption characteristics...
February 2, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28167352/evaluation-of-amendments-to-reduce-arsenic-and-antimony-leaching-from-co-contaminated-soils
#20
Steven J Doherty, Matthew K Tighe, Susan C Wilson
Co-contamination of soils with arsenic (As) and antimony (Sb) presents unique challenges for risk management. In this study a sequence of leaching experiments evaluated zero valent iron powder, ferrihydrite, ferric chloride, aluminium and manganese oxides, and kaolinite for As and Sb immobilisation in co-contaminated soils. Iron based amendments were most effective for the reduction of As and Sb in leachate in a column leaching study. Over 48 h zero valent iron powder and ferrihydrite applied at 3% (w/w dry weight) were most efficient, decreasing total As and Sb leachate concentrations by more than 80%...
January 23, 2017: Chemosphere
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