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Immobilisation

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https://www.readbyqxmd.com/read/29234015/high-resolution-single-particle-zeta-potential-characterisation-of-biological-nanoparticles-using-tunable-resistive-pulse-sensing
#1
Robert Vogel, Anoop K Pal, Siddharth Jambhrunkar, Pragnesh Patel, Sachin S Thakur, Eduardo Reátegui, Harendra S Parekh, Paula Saá, Adonis Stassinopoulos, Murray F Broom
Physicochemical properties of nanoparticles, such as size, shape, surface charge, density, and porosity play a central role in biological interactions and hence accurate determination of these characteristics is of utmost importance. Here we propose tunable resistive pulse sensing for simultaneous size and surface charge measurements on a particle-by-particle basis, enabling the analysis of a wide spectrum of nanoparticles and their mixtures. Existing methodologies for measuring zeta potential of nanoparticles using resistive pulse sensing are significantly improved by including convection into the theoretical model...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29229178/cervical-spine-clearance-in-adults-following-blunt-trauma-a-national-survey-across-major-trauma-centres-in-england
#2
G Chilvers, K Porter, S Choudhary
AIM: To assess current practice in cervical spine clearance across major trauma centres in England and review current guidelines. MATERIALS AND METHODS: An electronic survey was sent to lead trauma clinicians at 22 major trauma centres in England. This assessed the clinical decision tools used, the choice of initial imaging technique, and the methods used to clear the cervical spine in alert and obtunded patients without focal neurology, and where initial imaging was normal...
December 8, 2017: Clinical Radiology
https://www.readbyqxmd.com/read/29227129/assessment-of-a-portable-lactate-meter-for-field-use-in-the-white-rhinoceros-i-ceratotherium-simum-i
#3
Georgina C Cole, Adrian S W Tordiffe, Gerhard Steenkamp
Blood lactate is a predictor of mortality in critically ill humans and animals. Handheld lactate meters have the potential to be used in the field to evaluate the condition of severely injured rhinoceroses but have not been compared with laboratory-based methods. Agreement between a handheld lactate meter and a laboratory method was assessed, as was the stability of rhino blood lactate in the anticoagulant sodium fluoride/potassium oxalate (fluoride/oxalate). Blood samples were obtained from 53 white rhinos that had been immobilised for management reasons...
November 10, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/29224930/transport-of-dissolved-organic-matter-in-boom-clay-size-effects
#4
D Durce, M Aertsens, D Jacques, N Maes, M Van Gompel
A coupled experimental-modelling approach was developed to evaluate the effects of molecular weight (MW) of dissolved organic matter (DOM) on its transport through intact Boom Clay (BC) samples. Natural DOM was sampled in-situ in the BC layer. Transport was investigated with percolation experiments on 1.5cm BC samples by measuring the outflow MW distribution (MWD) by size exclusion chromatography (SEC). A one-dimensional reactive transport model was developed to account for retardation, diffusion and entrapment (attachment and/or straining) of DOM...
December 5, 2017: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/29220155/superchiral-plasmonic-phase-sensitivity-for-fingerprinting-of-protein-interface-structure
#5
Ryan Tullius, Geoffrey W Platt, Larousse Khosravi Khorashad, Nikolaj Gadegaard, Adrian J Lapthorn, Vincent M Rotello, Graeme Cooke, Laurence D Barron, Alexander O Govorov, Affar Shahid Karimullah, Malcolm Kadodwala
The structure adopted by biomaterials, such as proteins, at interfaces is a crucial parameter in a range of important biological problems. It is a critical property in defining the functionality of cell / bacterial membranes, biofilms (i.e. in antibiotic resistant infections) and the exploitation of immobilised enzymes in biocatalysis. The intrinsically small quantities of materials at interfaces precludes the application of conventional spectroscopic phenomena routinely used for (bio)structural analysis due to a lack of sensitivity...
December 8, 2017: ACS Nano
https://www.readbyqxmd.com/read/29217349/arthrodesis-of-the-proximal-interphalangeal-joint-of-the-4th-and-5th-finger-using-an-interlocking-screw-device-to-treat-severe-recurrence-of-dupuytren-s-disease
#6
C D Novoa-Parra, D Montaner-Alonso, J I Pérez-Correa, J Morales-Rodríguez, J L Rodrigo-Pérez, M Morales-Suarez-Varela
OBJECTIVE: To assess the radiological and functional outcome of arthrodesis of the 4th and 5th finger using the APEX™ (Extremity Medical, Parsippany,NJ)intermedullary interlocking screw system in patients with severe recurrence of Dupuytren's disease. MATERIAL AND METHOD: The DASH questionnaire and the VAS scale were used to assess the clinical outcomes. The angle of arthrodesis, fusion time and implant fixation were evaluated on x-rays. The patients were monitored for complications during surgery and the follow-up period...
December 4, 2017: Revista Española de Cirugía Ortopédica y Traumatología
https://www.readbyqxmd.com/read/29215782/the-effect-of-urinary-foley-catheter-substrate-material-on-the-antimicrobial-potential-of-calixerene-based-molecules
#7
A Guildford, C Morris, O Kitt, I Cooper
AIMS: This study was to investigate the antimicrobial activity of a modified calixarene polymer bound to a silicone substrate in the presence of pathogens associated with catheter infections, Escherichia coli and Proteus mirabilis. METHODS AND RESULTS: The molecule and its constituent parts, were studied bound and unbound to silicone substrates to ascertain growth effects. Minimum inhibitory and bactericidal concentrations were determined against E.coli and Pr.mirabilis...
December 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29212528/effectiveness-of-robot-assisted-training-added-to-conventional-rehabilitation-in-patients-with-humeral-fracture-early-after-surgical-treatment-protocol-of-a-randomised-controlled-multicentre-trial
#8
Corinna Nerz, Lars Schwickert, Clemens Becker, Stefan Studier-Fischer, Janina Anna Müßig, Peter Augat
BACKGROUND: The incidence of proximal humeral fractures increases with age. The functional recovery of the upper arm after such fractures is slow, and results are often disappointing. Treatment is associated with long immobilisation periods. Evidence-based exercise guidelines are missing. Loss of muscle mass as well as reduced range of motion and motor performance are common consequences. These losses could be partly counteracted by training interventions using robot-assisted arm support of the affected arm derived from neurorehabilitation...
December 6, 2017: Trials
https://www.readbyqxmd.com/read/29200659/electrochemical-detection-of-urinary-micrornas-via-sulfonamide-bound-antisense-hybridisation
#9
Daniel A Smith, Lucy J Newbury, Guido Drago, Timothy Bowen, James E Redman
Altered serum and plasma microRNA (miRNA) expression profiles have been observed in numerous human diseases, with a number of studies describing circulating miRNA biomarkers for cancer diagnosis, prognosis and response to treatment, and recruitment to clinical trials for miRNA-based drug therapy already underway. Electrochemical detection of biomarkers in urine has several significant advantages over circulating biomarker analysis including safety, cost, speed and ease of conversion to the point of care environment...
December 2017: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/29197839/rupture-of-the-posterior-cul-de-sac-during-trial-of-labour-after-caesarean-section
#10
Lysanne D C Graafmans, Marja G K Dijksterhuis, Louwerens D Vos, Jeroen van Bavel
Rupture of the vaginal wall in unobstructed labour is a rare entity in the developed world. This case report describes rupture of the posterior cul-de-sac in a healthy 34-year-old multiparous woman attempting trial of labour after caesarean section. The woman presented to the labour ward at term with spontaneous onset of contractions. In the second stage of labour, the woman experienced sudden severe abdominal pain, different in character from the contraction pain. Therefore, the baby was delivered by ventouse extraction...
December 2, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29197793/native-plant-amendments-and-topsoil-addition-enhance-soil-function-in-post-mining-arid-grasslands
#11
Tayla Kneller, Richard J Harris, Amber Bateman, Miriam Muñoz-Rojas
One of the most critical challenges faced in restoration of disturbed arid lands is the limited availability of topsoil. In post-mining restoration, alternative soil substrates such as mine waste could be an adequate growth media to alleviate the topsoil deficit, but these materials often lack appropriate soil characteristics to support the development and survival of seedlings. Thus, addition of exogenous organic matter may be essential to enhance plant survival and soil function. Here, we present a case study in the arid Pilbara region (north-west Western Australia), a resource-rich area subject to intensive mining activities...
November 30, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29188265/forged-and-fashioned-for-faithfulness-ruthenium-olefin-metathesis-catalysts-bearing-ammonium-tags
#12
Anupam Jana, Karol Grela
In this article, the synthesis and applications of selected ammonium tagged Ru-alkylidene metathesis catalysts were described. Because of the straightforward synthesis, the first generation of onium-tagged catalysts have the ammonium group installed in the benzylidene ligand. Such catalysts usually give relatively pure metathesis products, and are used in polar solvents and water, or immobilised on various supports. Later, catalysts tagged in the N-heterocyclic carbene ligand (NHC) were developed to offer higher stability and even lower metal contamination levels...
November 30, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29187035/immobilisation-of-hydroxyapatite-collagen-on-polydopamine-grafted-stainless-steel-316l-coating-adhesion-and-in-vitro-cells-evaluation
#13
Zafirah Tapsir, Farah H Jamaludin, Belinda Pingguan-Murphy, Syafiqah Saidin
The utilisation of hydroxyapatite and collagen as bioactive coating materials could enhance cells attachment, proliferation and osseointegration. However, most methods to form crystal hydroxyapatite coating do not allow the incorporation of polymer/organic compound due to production phase of high sintering temperature. In this study, a polydopamine film was used as an intermediate layer to immobilise hydroxyapatite-collagen without the introduction of high sintering temperature. The surface roughness, coating adhesion, bioactivity and osteoblast attachment on the hydroxyapatite-collagen coating were assessed as these properties remains unknown on the polydopamine grafted film...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29179907/management-and-outcomes-of-isolated-paediatric-tibial-spine-fractures
#14
A B Scrimshire, M Gawad, R Davies, H George
Paediatric fractures of the tibial spine are relatively rare and controversy remains around how these injuries are best managed (Gans et al., 2014; Hargrove et al., 2004). Consequently most non-specialised paediatric units have limited experience of managing these injuries. This study aims to review the management and outcomes of isolated paediatric tibial spine fractures in a tertiary paediatric orthopaedic centre between 2008 and 2016. Data were collected on patient demographics, mechanism of injury, imaging, Meyers and McKeever grade of injury (Meyers and McKeever), management and outcomes, including Cincinnati and Lysholm-Tegner knee scores at a mean of 36 months post-operatively...
November 16, 2017: Injury
https://www.readbyqxmd.com/read/29179070/biochar-modulates-heavy-metal-toxicity-and-improves-microbial-carbon-use-efficiency-in-soil
#15
Yilu Xu, Balaji Seshadri, Binoy Sarkar, Hailong Wang, Cornelia Rumpel, Donald Sparks, Mark Farrell, Tony Hall, Xiaodong Yang, Nanthi Bolan
Soil organic carbon is essential to improve soil fertility and ecosystem functioning. Soil microorganisms contribute significantly to the carbon transformation and immobilisation processes. However, microorganisms are sensitive to environmental stresses such as heavy metals. Applying amendments, such as biochar, to contaminated soils can alleviate the metal toxicity and add carbon inputs. In this study, Cd and Pb spiked soils treated with macadamia nutshell biochar (5% w/w) were monitored during a 49days incubation period...
November 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29174400/self-assembled-monolayer-based-immunoassays-for-okadaic-acid-detection-in-seawater-as-monitoring-tools
#16
Sandra Leonardo, Anna Toldrà, Maria Rambla-Alegre, Margarita Fernández-Tejedor, Karl B Andree, Laura Ferreres, Katrina Campbell, Christopher T Elliott, Ciara K O'Sullivan, Yolanda Pazos, Jorge Diogène, Mònica Campàs
Rapid and cost-effective methods to monitor the presence of diarrhetic shellfish poisoning (DSP) toxins in seawater samples in an easy and reliable manner are required to protect human health and avoid economic losses to shellfish industry. Immunoassays for the detection of okadaic acid (OA) and dinophysistoxin-1 and dinophysistoxin-2 are developed by immobilising OA on self-assembled monothiols or dithiols in an ordered and oriented way, providing an effective limit of detection of ∼1 ng OA equiv./mL seawater...
November 14, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/29169130/can-liming-reduce-cadmium-cd-accumulation-in-rice-oryza-sativa-in-slightly-acidic-soils-a-contradictory-dynamic-equilibrium-between-cd-uptake-capacity-of-roots-and-cd-immobilisation-in-soils
#17
Yongjie Yang, Jiangmin Chen, Qina Huang, Shaoqing Tang, Jianlong Wang, Peisong Hu, Guosheng Shao
Cadmium (Cd) accumulation in rice is strongly controlled by liming, but information on the use of liming to control Cd accumulation in rice grown in slightly acidic soils is inconsistent. Here, pot experiments were carried out to investigate the mechanisms of liming on Cd accumulation in two rice varieties focusing on two aspects: available/exchangeable Cd content in soils that were highly responsive to liming, and Cd uptake and transport capacity in the roots of rice in terms of Cd accumulation-relative gene expression...
November 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/29161985/fatty-acid-profile-from-immobilised-chlorella-vulgaris-cells-in-different-matrices
#18
Noor Raihana Abu Sepian, Nur Hidayah Mat Yasin, Norazwina Zainol, Nur Hanani Rushan, Abdul Latif Ahmad
The immobilisation of Chlorella vulgaris 211/11B entrapped in combinations of natural matrices to simplify the harvesting process was demonstrated in this study. Three combinations of matrices were investigated comprised of calcium alginate (CA) and sodium alginate (SA), sodium carboxymethyl cellulose (CMC) and SA, and mixed matrices (SA, CA, and CMC). The number of cells growth for each immobilised matrix to microalgae volume ratios (0.2:1-1:1) were explored and compared with using SA solely as a control. The optimum volume ratios obtained were 1:1 for SA, 0...
November 21, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29156331/may-humic-acids-or-mineral-fertilisation-mitigate-arsenic-mobility-and-availability-to-carrot-plants-daucus-carota-l-in-a-volcanic-soil-polluted-by-as-from-irrigation-water
#19
Antonio G Caporale, Paola Adamo, Shah M G G Azam, Maria A Rao, Massimo Pigna
Carrot (Daucus carota L.) is a widely consumed root vegetable, whose growth and safety might be threatened by growing-medium arsenic (As) contamination. By this work, we evaluated the effects of humic acids from Leonardite and NPK mineral fertilisation on As mobility and availability to carrot plants grown for 60 days in a volcanic soil irrigated with As-contaminated water - representing the most common scenario occurring in As-affected Italian areas. As expected, the irrigation with As-contaminated water caused a serious toxic effect on plant growth and photosynthetic rate; the highest rate of As also inhibited soil enzymatic activity...
November 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/29155402/synthesis-of-silver-nanoparticles-from-a-desmodium-adscendens-extract-and-its-antibacterial-evaluation-on-wound-dressing-material
#20
Jaya R Lakkakula, Derek Tantoh Ndinteh, Sandy F van Vuuren, Denise K Olivier, Rui W M Krause
The one-pot synthesis of silver nanoparticles (AgNPs) using the medium-polar extract of Desmodium adscendens (Sw.) DC. is presented here as an alternative synthesis of metal NPs. Characterisation of the formed NPs showed polydispersed AgNPs ranging from 15 to 100 nm where the concentration of metal ions was found to play a role in the size and shape of the prepared NPs. It could be established that the flavonoids, saponins, and alkaloids present in the extract acted as both reducing and stabilising agents during the formation of the capped metal NPs...
December 2017: IET Nanobiotechnology
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