keyword
MENU ▼
Read by QxMD icon Read
search

Immobilisation

keyword
https://www.readbyqxmd.com/read/28432344/aptamer-based-label-free-electrochemical-biosensor-array-for-the-detection-of-total-and-glycated-hemoglobin-in-human-whole-blood
#1
Shimaa Eissa, Mohammed Zourob
The increase of the level of glucose in blood leads to an increase in the fraction of glycated hemoglobin (HbA1c). Therefore, the percentage of HbA1c in the blood can serve as a marker for the average glucose level over the past three months and thus, it can be used to diagnose diabetes. Here, we report the selection, identification and characterization of specific DNA aptamers against HbA1c- and total hemoglobin (tHb) and their integration into an electrochemical array sensing platform. High affinity and specificity aptamers were selected in vitro showing dissociation constants of 2...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432339/multifunctional-substrate-of-label-free-electrochemical-immunosensor-for-ultrasensitive-detection-of-cytokeratins-antigen-21-1
#2
Huiqiang Wang, Xin Gao, Zhanfang Ma
Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at -0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28428037/conservative-treatment-of-displaced-distal-radius-fractures-leads-to-acceptable-functional-outcomes-however-at-the-expense-of-40-subsequent-surgeries
#3
Marjolein A M Mulders, Percy V van Eerten, J Carel Goslings, Niels W L Schep
BACKGROUND: Although secondary displacement following closed reduction and plaster immobilisation is high, several guidelines still recommend non-operative treatment for displaced distal radius fractures with an adequate closed reduction. PURPOSE: The purpose of this study was to evaluate functional outcomes, measured with the Disability of the Arm, Shoulder and Hand (DASH) questionnaire, in non-operative treated patients with displaced distal radius fractures with an adequate closed reduction confirmed on radiograph...
April 17, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28426217/phosphorus-depletion-as-a-green-alternative-to-biocides-for-controlling-biodegradation-of-metalworking-fluids
#4
Yaldah Azimi, Ian P Thompson
Metalworking fluids (MWFs) are used as lubricants and coolants in the manufacturing operations. Their biodeterioration, whilst in-operation, is a widespread problem leading to poor performance and worker health issues. Adding biocides, though effective in reducing microbial growth, leads to the production of more recalcitrant wastewaters that are difficult to dispose or recycle on-site. Increasing environmental concerns have led to robust legislation for reducing/eliminating the use of toxic biocides in MWFs, stimulating a growing interest in the development/application of alternative biological preservation strategies...
April 20, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28424235/increased-alloimmunisation-and-transfusion-reaction-reporting-in-patients-with-solid-phase-panreactivity
#5
Andrea M Olofson, Rachael M Chandler, Cynthia R Marx-Wood, Craig A Babcock, Nancy M Dunbar
BACKGROUND: Automated solid-phase antibody screening uses red blood cell (RBC) membranes immobilised on polystyrene test wells to detect RBC specific antibodies. Despite its time-saving and labour-saving benefits, this method produces a higher rate of nonspecific reactivity compared with manual screening. Solid-phase panreactivity (SPP) is characterised by panreactivity (ie, all test cells reacting) in solid-phase testing accompanied by a negative autocontrol and a lack of reactivity when the same screening cells are tested in tube...
April 19, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28397851/structural-and-magnetic-properties-of-multi-core-nanoparticles-analysed-using-a-generalised-numerical-inversion-method
#6
P Bender, L K Bogart, O Posth, W Szczerba, S E Rogers, A Castro, L Nilsson, L J Zeng, A Sugunan, J Sommertune, A Fornara, D González-Alonso, L Fernández Barquín, C Johansson
The structural and magnetic properties of magnetic multi-core particles were determined by numerical inversion of small angle scattering and isothermal magnetisation data. The investigated particles consist of iron oxide nanoparticle cores (9 nm) embedded in poly(styrene) spheres (160 nm). A thorough physical characterisation of the particles included transmission electron microscopy, X-ray diffraction and asymmetrical flow field-flow fractionation. Their structure was ultimately disclosed by an indirect Fourier transform of static light scattering, small angle X-ray scattering and small angle neutron scattering data of the colloidal dispersion...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28396838/thermo-kinetic-analysis-space-expansion-for-cyclophilin-ligand-interactions-identification-of-a-new-nonpeptide-inhibitor-using-biacore%C3%A2-t200
#7
Martin A Wear, Matthew W Nowicki, Elizabeth A Blackburn, Iain W McNae, Malcolm D Walkinshaw
We have established a refined methodology for generating surface plasmon resonance sensor surfaces of recombinant his-tagged human cyclophilin-A. Our orientation-specific stabilisation approach captures his-tagged protein under 'physiological conditions' (150 mm NaCl, pH 7.5) and covalently stabilises it on Ni(2+)-nitrilotriacetic acid surfaces, very briefly activated for primary amine-coupling reactions, producing very stable and active surfaces (≥ 95% specific activity) of cyclophilin-A. Variation in protein concentration with the same contact time allows straightforward generation of variable density surfaces, with essentially no loss of activity, making the protocol easily adaptable for studying numerous interactions; from very small fragments, ~ 100 Da, to large protein ligands...
April 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28395685/the-benefit-of-pharmacological-venous-thromboprophylaxis-in-foot-and-ankle-surgery
#8
N P Saragas, P N F Ferrao, B F Jacobson, E Saragas, A Strydom
BACKGROUND: Ten percent of patients with a deep-vein thrombosis (DVT) will develop a fatal pulmonary embolism (PE), often initially asymptomatic. The risks and benefits of pharmacological thromboprophylaxis are well documented in respect of total joint arthroplasty and hip fractures, but little is understood about the incidence of venous thromboembolism (VTE) or the potential risks and benefits of chemoprophylaxis in foot and ankle surgery. OBJECTIVE: To determine whether prophylactic chemoprophylaxis had any impact on the prevention of VTE in a cohort of foot and ankle surgical patients requiring the combination of below-knee cast immobilisation and non-weightbearing for ≥4 weeks...
March 29, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/28390557/immobilisation-in-australian-paediatric-medical-imaging-a-pilot-study
#9
S Noonan, K Spuur, S Nielsen
AIMS: The primary aim of this study is to document the use of paediatric immobilisation techniques in medical imaging. Secondary aims are to investigate differences between current practice of paediatric and non-paediatric facilities and radiographer gender and to investigate immobilisation protocols. METHODS: A SurveyMonkey link was distributed through the Australian Society of Medical Imaging and Radiation Therapy (ASMIRT) newsletter. Radiographer members of ASMIRT were invited to participate...
May 2017: Radiography
https://www.readbyqxmd.com/read/28384443/effect-of-platelet-derived-%C3%AE-thromboglobulins-on-coagulation
#10
Karl Egan, Johanna P van Geffen, Hui Ma, Barry Kevane, Aine Lennon, Seamus Allen, Elaine Neary, Martin Parsons, Patricia Maguire, Kieran Wynne, Richard O' Kennedy, Johan W M Heemskerk, Fionnuala Ní Áinle
BACKGROUND: β-thromboglobulins are derived from the cleavage of the CXC chemokine platelet basic protein and are released in high concentrations by activated platelets. Platelet-derived β-thromboglobulins (βTG) share 70% homology with platelet factor 4 (PF4), another CXC chemokine released by activated platelets. PF4 modulates coagulation by inhibiting heparin-antithrombin interactions, promoting protein C activation, and attenuating the activity of activated protein C. In contrast, the effect of βTG on coagulation is unknown...
March 30, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28378380/re-evaluation-of-insect-melanogenesis-research-views-from-the-dark-side
#11
REVIEW
Miranda M A Whitten, Christopher J Coates
Melanins (eumelanin and pheomelanin) are synthesised in insects for several purposes including cuticle sclerotisation and color patterning, clot formation, organogenesis and innate immunity. Traditional views of insect immunity detail the storage of pro-phenoloxidases inside specialized blood cells (hemocytes) and their release upon recognition of foreign bodies. Activated phenoloxidases convert monophenols into reactive quinones in a two-step enzymatic reaction, and until recently, the mechanism of tyrosine hydroxylation remained a mystery...
April 4, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28367070/characterisation-of-nanomaterial-hydrophobicity-using-engineered-surfaces
#12
Cloé Desmet, Andrea Valsesia, Arianna Oddo, Giacomo Ceccone, Valentina Spampinato, François Rossi, Pascal Colpo
Characterisation of engineered nanomaterials (NMs) is of outmost importance for the assessment of the potential risks arising from their extensive use. NMs display indeed a large variety of physico-chemical properties that drastically affect their interaction with biological systems. Among them, hydrophobicity is an important property that is nevertheless only slightly covered by the current physico-chemical characterisation techniques. In this work, we developed a method for the direct characterisation of NM hydrophobicity...
2017: Journal of Nanoparticle Research: An Interdisciplinary Forum for Nanoscale Science and Technology
https://www.readbyqxmd.com/read/28365390/investigation-of-in-vitro-pdt-activities-of-zinc-phthalocyanine-immobilised-tio2-nanoparticles
#13
Fatma Yurt, Mine Ince, Suleyman Gokhan Colak, Kasim Ocakoglu, Ozge Er, Hale Melis Soylu, Cumhur Gunduz, Cigir Biray Avci, Cansu Caliskan Kurt
Phthalocyanines (Pcs) are commonly used as Photosensors (PSs) in Photodynamic Therapy (PDT) applications due to their intense absorption in the far red-near IR spectral region with a high extinction coefficient and high ability for generating singlet oxygen. Pcs targetspecifically tumors, and do not show any considerable toxic effects under the absence of light. In particular, their chemical versatility has allowed the introducion a number of substituent at the periferal or axial positions which provide modulating photophysical properties, increases the solubility of these compounds in organic solvents...
March 29, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28362488/serotonin-transporter-associated-protein-complexes-are-enriched-in-synaptic-vesicle-proteins-and-proteins-involved-in-energy-metabolism-and-ion-homeostasis
#14
Jana Haase, Joanna Grudzinska-Goebel, Heidi Kaastrup Müller, Agnieszka Münster-Wandowski, Elysian Chow, Kieran Wynne, Zohreh Farsi, Johannes-Friedrich Zander, Gudrun Ahnert-Hilger
The serotonin transporter (SERT) mediates Na(+)-dependent high-affinity serotonin uptake and plays a key role in regulating extracellular serotonin concentration in the brain and periphery. To gain novel insight into SERT regulation, we conducted a comprehensive proteomics screen to identify components of SERT-associated protein complexes in the brain by employing three independent approaches. In vivo SERT complexes were purified from rat brain using an immobilized high-affinity SERT ligand, amino-methyl citalopram...
April 21, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28352534/the-morphology-of-ganoderma-lucidum-mycelium-in-a-repeated-batch-fermentation-for-exopolysaccharide-production
#15
Wan Abd Al Qadr Imad Wan-Mohtar, Safuan Ab Kadir, Nazamid Saari
The morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morphology was disposed to EPS production in the shake flask and bioreactor, respectively. Seven RBF could be carried out in 500 mL flasks, and five repeated batches were performed in a 2 L bioreactor...
September 2016: Biotechnology Reports
https://www.readbyqxmd.com/read/28352523/immobilisation-of-cyclodextrin-glucanotransferase-into-polyvinyl-alcohol-pva-nanofibres-via-electrospinning
#16
Suryani Saallah, M Nazli Naim, I Wuled Lenggoro, Mohd Noriznan Mokhtar, Noor Fitrah Abu Bakar, Masao Gen
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2...
June 2016: Biotechnology Reports
https://www.readbyqxmd.com/read/28343136/facile-microfluidic-production-of-composite-polymer-core-shell-microcapsules-and-crescent-shaped-microparticles
#17
Ekanem E Ekanem, Zilin Zhang, Goran T Vladisavljević
HYPOTHESIS: Core-shell microcapsules and crescent-shaped microparticles can be used as picolitre bioreactors for cell culture and microwells for cell trapping/immobilisation, respectively. RESULTS: Monodisperse polylactic acid (PLA) core-shell microcapsules with a diameter above 200μm, a shell thickness of 10μm, and 96% water entrapment efficiency were produced by solvent evaporation from microfluidically generated W/O/W emulsion drops with core-shell structure, and used to encapsulate Saccharomyces cerevisiae yeast cells in their aqueous cores...
March 18, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28337069/detection-of-alien-genetic-introgressions-in-bread-wheat-using-dot-blot-genomic-hybridisation
#18
María-Dolores Rey, Pilar Prieto
Simple, reliable methods for the identification of alien genetic introgressions are required in plant breeding programmes. The use of genomic dot-blot hybridisation allows the detection of small Hordeum chilense genomic introgressions in the descendants of genetic crosses between wheat and H. chilense addition or substitution lines in wheat when molecular markers are difficult to use. Based on genomic in situ hybridisation, DNA samples from wheat lines carrying putatively H. chilense introgressions were immobilised on a membrane, blocked with wheat genomic DNA and hybridised with biotin-labelled H...
2017: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/28335969/design-synthesis-and-application-of-a-new-class-of-stimuli-responsive-separation-materials
#19
Roshanak Sepehrifar, Reinhard I Boysen, Basil Danylec, Yuanzhong Yang, Kei Saito, Milton T W Hearn
A new class of efficient stationary phase has been investigated for use in the liquid chromatographic separation of low molecular weight analytes and high molecular weight biomolecules, based on the application of immobilised stimuli-responsive polymers (SRPs). To this end, two polymeric units, namely poly(2-dimethylaminoethyl methacrylate) (PDMAEMA) and poly(acrylic acid) (PAA) were tethered to a triazine core. The derived poly(2-dimethyl-aminoethyl methacrylate)-block-poly(acrylic acid) (PDMAEMA-b-PAA), as a diblock co-polymer, was then immobilised onto the surface of porous silica particles...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28331932/pulmonary-embolism-and-in-situ-pulmonary-artery-thrombosis-in-paediatrics-a-systematic-review
#20
Madhvi Rajpurkar, Tina Biss, Ernest Amankwah, Denise Martinez, Suzan Williams, C Heleen Van Ommen, Neil A Goldenberg
Data on paediatric pulmonary embolism (PE) are scarce. We sought to systematically review the current literature on childhood PE and conducted a search on paediatric PE via PubMed (1946-2013) and Embase (1980-2013). There was significant heterogeneity in reported data. Two patterns were noted: classic thromboembolic PE (TE-PE) and in situ pulmonary artery thrombosis (ISPAT). Mean age of presentation for TE-PE was 14.86 years, and 51 % of cases were males. The commonest method for diagnosis of TE-PE was contrast CT with angiography (74 % of patients)...
March 23, 2017: Thrombosis and Haemostasis
keyword
keyword
63323
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"