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https://www.readbyqxmd.com/read/28091676/hemispherical-platinum%C3%A2-%C3%A2-silver-core%C3%A2-%C3%A2-shell-nanoparticles-for-mirna-detection
#1
Elaine Spain, Kellie Adamson, Mohammad Elshahawy, Isabella Bray, Tia E Keyes, Raymond L Stallings, Robert J Forster
Defects within a self-assembled monolayer (SAM) of dodecanethiol on gold have been used as nucleation sites for the electrodeposition of mushroom shaped platinum nanoparticles (PtNPs). The top surfaces of these PtNPs were then decorated with a layer of silver creating a hemispherical - platinum : silver core : shell nanoparticle (Pt-AgNP). Thiolated probe strand miRNA was then immobilised onto the upper silver surface. These regioselectively modified particles were desorbed by applying a current jump to yield nanoparticles capable of hybridising to a complementary miRNA target with electrocatalysis occurring on the non-functionalized lower surface...
January 16, 2017: Analyst
https://www.readbyqxmd.com/read/28067647/decomposition-of-xenobiotics-during-visible-light-irradiation-in-the-presence-of-immobilised-photosensitisers-kinetics-study
#2
M Olak-Kucharczyk, M Foszpańczyk, M Gmurek, S Ledakowicz
The objective of this work was to study the photosensitised oxidation of the xenobiotics benzylparaben (BeP) and 2,4dichlorophenol (2,4DCP) in aqueous solutions using photosensitisers immobilised into chitosan carrier particles and visible light radiation. Zn(II) phthalocyanine tetrasulfonate tetrasodium salt and Al(III) phthalocyanine chloride tetrasulfonic acid were used as photosensitisers. The major role of the singlet oxygen during photodegradation was proven by using scavengers of reactive oxygen species...
January 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28057558/characterization-of-the-planktonic-microbiome-in-upflow-anaerobic-sludge-blanket-reactors-during-adaptation-of-mesophilic-methanogenic-granules-to-thermophilic-operational-conditions
#3
Xinyu Zhu, Laura Treu, Panagiotis G Kougias, Stefano Campanaro, Irini Angelidaki
Upflow anaerobic sludge blanket (UASB) technology refers to reactor technology where granules, i.e. self-immobilised microbial associations, are the biological catalysts involved in the anaerobic digestion process. During the start-up period, UASB reactors operate at relatively long HRT and therefore the liquid phase of the reactor becomes a favourable environment for microbial growth. The current study aimed to elucidate the dynamicity of the suspended microbial community in UASB reactors, during the transition from mesophilic to thermophilic conditions...
January 2, 2017: Anaerobe
https://www.readbyqxmd.com/read/28053266/predictors-of-mortality-following-conservatively-managed-fractures-of-the-odontoid-in-elderly-patients
#4
S Bajada, A Ved, A G Dudhniwala, S Ahuja
AIMS: Rates of mortality as high as 25% to 30% have been described following fractures of the odontoid in the elderly population. The aim of this study was to examine whether easily identifiable variables present on admission are associated with mortality. PATIENTS AND METHODS: A consecutive series of 83 elderly patients with a fracture of the odontoid following a low-impact injury was identified retrospectively. Data that were collected included demographics, past medical history and the results of blood tests on admission...
January 2017: Bone & Joint Journal
https://www.readbyqxmd.com/read/28052526/effectiveness-of-a-multi-layer-foam-dressing-in-preventing-sacral-pressure-ulcers-for-the-early-acute-care-of-patients-with-a-traumatic-spinal-cord-injury-comparison-with-the-use-of-a-gel-mattress
#5
Andréane Richard-Denis, Cynthia Thompson, Jean-Marc Mac-Thiong
Individuals with spinal cord injury are at risk of sacral pressure ulcers due to, among other reasons, prolonged immobilisation. The effectiveness of a multi-layer foam dressing installed pre-operatively in reducing sacral pressure ulcer occurrence in spinal cord injured patients was compared to that of using a gel mattress, and stratified analyses were performed on patients with complete tetraplegia and paraplegia. Socio-demographic and clinical data were collected from 315 patients admitted in a level-I trauma centre following a spinal cord injury between April 2010 and March 2016...
January 4, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28052090/interaction-between-antibacterial-peptide-apep10-and-escherichia-coli-membrane-lipids-evaluated-using-liposome-as-pseudo-stationary-phase
#6
Wenting Tang, Chuanfen Pu, Man Li
Liposomes constructed from Escherichia coli membrane lipids were used as a pseudo-stationary phase in capillary electrophoresis and immobilised liposome chromatography to evaluate the interaction between antibacterial peptide (ABP) Apep10 and bacterial membrane lipids. The peptide mobility decreased as the concentration of liposomes increased, providing evidence for the existence of this interaction. The binding constant between Apep10 and the Escherichia coli membranes lipid liposome was higher than that of Apep10 with a mixed phospholipids liposome at the same temperature...
2017: PloS One
https://www.readbyqxmd.com/read/28043852/surgical-techniques-for-lumbo-sacral-fusion
#7
REVIEW
P Tropiano, H Giorgi, A Faure, B Blondel
Lumbo-sacral (L5-S1) fusion is a widely performed procedure that has become the reference standard treatment for refractory low back pain. L5-S1 is a complex transition zone between the mobile lordotic distal lumbar spine and the fixed sacral region. The goal is to immobilise the lumbo-sacral junction in order to relieve pain originating from this site. Apart from achieving inter-vertebral fusion, the main challenge lies in the preoperative determination of the fixed L5-S1 position that will be optimal for the patient...
December 30, 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28002769/sorbent-amendment-as-a-remediation-strategy-to-reduce-pfas-mobility-and-leaching-in-a-contaminated-sandy-soil-from-a-norwegian-firefighting-training-facility
#8
Sarah E Hale, Hans Peter H Arp, Gøril Aasen Slinde, Emma Jane Wade, Kamilla Bjørseth, Gijs D Breedveld, Bengt Fredrik Straith, Kamilla Grotthing Moe, Morten Jartun, Åse Høisæter
Aqueous film-forming foams (AFFF) containing poly- and perfluoroalkyl substances (PFAS) used for firefighting have led to the contamination of soil and water at training sites. The unique physicochemical properties of PFAS results in environmental persistency, threatening water quality and making remediation of such sites a necessity. This work investigated the role of sorbent amendment to PFAS contaminated soils in order to immobilise PFAS and reduce mobility and leaching to groundwater. Soil was sampled from a firefighting training facility at a Norwegian airport and total and leachable PFAS concentrations were quantified...
December 13, 2016: Chemosphere
https://www.readbyqxmd.com/read/27999851/omega-3-fatty-acids-and-vitamin-d-in-immobilisation-part-b-modulation-of-muscle-functional-vascular-and-activation-profiles
#9
E L Bostock, C I Morse, K Winwood, I M McEwan, G L Onambélé
OBJECTIVES: This study set out to determine whether two potential protein-sparing modulators (eicosapentaenoic acid and vitamin D) would modulate the anticipated muscle functional and related blood vessels function deleterious effects of immobilisation. DESIGN: The study used a randomised, double-blind, placebo-controlled design. SETTING: The study took part in a laboratory setting. PARTICIPANTS: Twenty-four male and female healthy participants, aged 23...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/27999850/omega-3-fatty-acids-and-vitamin-d-in-immobilisation-part-a-modulation-of-appendicular-mass-content-composition-and-structure
#10
E L Bostock, C I Morse, K Winwood, I M McEwan, G L Onambélé
OBJECTIVES: Muscle size decreases in response to short-term limb immobilisation. This study set out to determine whether two potential protein-sparing modulators (eicosapentaenoic acid and vitamin D) would attenuate immobilisation-induced changes in muscle characteristics. DESIGN: The study used a randomised, double-blind, placebo-controlled design. SETTING: The study took part in a laboratory setting. PARTICIPANTS: Twenty-four male and female healthy participants, aged 23...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/27998039/a-randomised-comparison-of-three-different-immobilisation-devices-for-thoracic-and-abdominal-cancers
#11
Catherine Hubie, Maddison Shaw, Sean Bydder, Jonny Lane, Gemma Waters, Megan McNabb, Rachel Kearvell, Alicia Concannon, Chrianna Bharat, Rob Appleyard
INTRODUCTION: Patient immobilisation is critically important for both highly conformal conventionally fractionated radiotherapy and for stereotactic body radiotherapy. Different immobilisation devices are available to maintain patient position for radiotherapy but the most suitable one remains unknown. METHODS: Forty-five patients were randomly allocated to one of three immobilisation devices; the Q fix arm shuttle, BodyFIX without wrap or BodyFIX with wrap. Patients were imaged before and after treatment to ascertain intra-fraction and inter-fraction motion...
December 20, 2016: Journal of Medical Radiation Sciences
https://www.readbyqxmd.com/read/27994637/surface-based-molecular-self-assembly-langmuir-blodgett-films-of-amphiphilic-ln-iii-complexes
#12
REVIEW
Dominic J Wales, Jonathan A Kitchen
The unique photophysical properties of the Ln(III) series has led to significant research efforts being directed towards their application in sensors. However, for "real-life" applications, these sensors should ideally be immobilised onto surfaces without loss of function. The Langmuir-Blodgett (LB) technique offers a promising method in which to achieve such immobilisation. This mini-review focuses on synthetic strategies for film formation, the effect that film formation has on the physical properties of the Ln(III) amphiphile, and concludes with examples of Ln(III) LB films being used as sensors...
2016: Chemistry Central Journal
https://www.readbyqxmd.com/read/27991634/interfacial-properties-and-emulsification-performance-of-thylakoid-membrane-fragments
#13
A Tamayo Tenorio, E W M de Jong, C V Nikiforidis, R M Boom, A J van der Goot
Thylakoids membranes are sophisticated, dynamic structures found in plant leaves, composed of protein complexes in a dynamic lipid matrix. The interfacial absorption dynamics and viscoelasticity of thylakoid membranes fragments were measured to assess the properties of the interfacial layer and to elucidate an emulsifying mechanism that includes the role of thylakoid's composition and 3D structure. Thylakoid membranes were extracted from sugar beet leaves by a series of buffer washing, filtration and centrifugation...
January 18, 2017: Soft Matter
https://www.readbyqxmd.com/read/27981632/proof-of-principle-immobilisation-of-robust-cu-ii-3-tb-iii-macrocycles-on-small-suitably-pre-functionalised-gold-nanoparticles
#14
Humphrey L C Feltham, Christophe Dumas, Matteo Mannini, Edwige Otero, Philippe Sainctavit, Roberta Sessoli, Carla J Meledandri, Sally Brooker
In a proof-of-principle study, a soluble macrocyclic single-molecule magnet (SMM) containing a Cu(II)3 Tb(III) magnetic core was covalently grafted onto small gold nanoparticles pre-functionalised with carboxylate-terminated tethers. A modified microemulsion method allowed production of the small and monodisperse nanoparticles (approximately 3.5 nm in diameter) for the chemisorption of a large amount of intact macrocyclic complexes in the hybrid system.
November 6, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27981574/loop-g-in-the-gabaa-receptor-%C3%AE-1-subunit-influences-gating-efficacy
#15
Daniel T Baptista-Hon, Simona Gulbinaite, Tim G Hales
The modification of Cys residues (substituted for D43 and T47) by 2-aminoethyl methanethiosulfonate in the GABAA α1 subunit loop G impairs activation of α1β2γ2 receptors by GABA and propofol (Baptista-Hon et al. 4). While the T47C substitution had no significant effect, non-conservative substitution of either residue (D43C and T47R) reduced the apparent potency of GABA. Propofol (1 μm), which potentiates sub-maximal, but not maximal GABA-evoked currents mediated by α1β2γ2 receptors, also potentiated maximal currents mediated by α1(D43C)β2γ2 and α1(T47R)β2γ2 receptors...
December 16, 2016: Journal of Physiology
https://www.readbyqxmd.com/read/27974856/functional-morphology-of-the-arm-spine-joint-and-adjacent-structures-of-the-brittlestar-ophiocomina-nigra-echinodermata-ophiuroidea
#16
Iain C Wilkie
The skeletal morphology of the arm spine joint of the brittlestar Ophiocomina nigra was examined by scanning electron microscopy and the associated epidermis, connective tissue structures, juxtaligamental system and muscle by optical and transmission electron microscopy. The behaviour of spines in living animals was observed and two experiments were conducted to establish if the spine ligament is mutable collagenous tissue: these determined (1) if animals could detach spines to which plastic tags had been attached and (2) if the extension under constant load of isolated joint preparations was affected by high potassium stimulation...
2016: PloS One
https://www.readbyqxmd.com/read/27974389/structure-and-lipid-binding-properties-of-the-kindlin-3-pleckstrin-homology-domain
#17
Tao Ni, Antreas Kalli, Fiona Naughton, Luke Yates, Omar Naneh, Mirijam Kozorog, Gregor Anderluh, Mark Sansom, Robert Gilbert
Kindlins co-activate integrins alongside talin. They possess, like talin, a FERM domain comprising F0-F3 subdomains, but with a pleckstrin homology (PH) domain inserted in the F2 subdomain that enables membrane association. We present the crystal structure of murine kindlin-3 PH domain determined at 2.23Å resolution and characterise its lipid binding using biophysical and computational approaches. Molecular dynamics (MD) simulations suggest flexibility in the PH domain loops connecting β-strands forming the putative phosphatidylinositol phosphate (PtdInsP) binding site...
December 14, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27959378/exploring-nanoparticle-porosity-using-nano-impacts-platinum-nanoparticle-aggregates
#18
Xue Jiao, Stanislav V Sokolov, Eden E L Tanner, Neil P Young, Richard G Compton
The porosity of platinum nanoparticles (PtNPs) is explored for the first time using tag-redox coulometry (TRC). This is achieved by monitoring the reduction of the 4-nitrobenzenethiol (NTP)-tagged PtNPs on carbon electrodes via both immobilisation and nanoimpacts. The average charge per impact is measured and attributed to the reduction of NTP adsorbed on individual PtNPs. The number of NTP molecules and thus the "active surface area" of the PtNPs is calculated and compared with two models: fully solid and porous nanoparticles, and the extent of the particle porosity is revealed...
December 21, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27931714/host-plant-growth-promotion-and-cadmium-detoxification-in-solanum-nigrum-mediated-by-endophytic-fungi
#19
Abdur Rahim Khan, Ihsan Ullah, Muhammad Waqas, Gun-Seok Park, Abdul Latif Khan, Sung-Jun Hong, Rehman Ullah, Byung Kwon Jung, Chang Eon Park, Shafiq Ur-Rehman, In-Jung Lee, Jae-Ho Shin
Current investigation conducted to evaluate the associated fungal endophyte interactions of a Cd hyper-accumulator Solanum nigrum Korean ecotype under varying concentrations of Cd. Two indole-3-acetic acid (IAA) producing fungal strains, RSF-4L and RSF-6L, isolated from the leaves of S. nigrum, were initially screened for Cd tolerance and accumulation potential. In terms of dry biomass production, the strain RSF-6L showed higher tolerance and accumulation capacity for Cd toxicity in comparison to RSF-4L. Therefore, RSF-6L was applied in vivo to S...
February 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27922431/a-multicentre-study-of-the-precision-and-accuracy-of-the-truslice-and-truslice-digital-histological-dissection-devices
#20
G E Orchard, M Shams, T Nwokie, P Fernando, C Bulut, C J Quaye, J Gabriel, Z Ramji, A Georgaki, M Watt, Z Cole, K Stewart, V McTaggart, S Padayachy, A M Long, A Ogden, C Andrews, A Birchall, F Shams, H Neesam, N Haine
BACKGROUND: Five key factors enabling a good surgical grossing technique include a flat uniformly perpendicular specimen cutting face, appropriate immobilisation of the tissue specimen during grossing, good visualisation of the cutting tissue face, sharp cutting knives and the grossing knife action. TruSlice and TruSlice Digital are new innovative tools based on a guillotine configuration. The TruSlice has plastic inserts whilst the TruSlice Digital has an electronic micrometre attached: both features enable these dissection factors to be controlled...
October 2016: British Journal of Biomedical Science
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