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https://www.readbyqxmd.com/read/28326617/entrapment-of-dyp-type-peroxidase-from-pseudomonas-fluorescens-pf-5-into-ca-alginate-magnetic-beads
#1
Agata Wasak, Radosław Drozd, Łukasz Struk, Bartłomiej Grygorcewicz
The aim of this study was to investigate the optimal conditions for the immobilisation and stabilisation of DyP1B Dye-decolourising peroxidases type B (DyP1B) from Pseudomonas fluorescens Pf-5 immobilised in Ca-alginate ferromagnetic beads (Ca-alginate FeBs). The immobilised DyP1B was used in the degradation of the Reactive Blue 5 synthetic dye. The enzyme was successfully entrapped in a Ca-alginate matrix, and showed an encapsulation efficiency of 94%. The concentration of 0.8 mg mL(-1) DyP1B, 2% of alginate and 10 mg mL(-1) magnetite were optimal for immobilisation...
March 21, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28303746/novel-design-and-optimisation-of-a-nitritation-anammox-setup-for-ammonium-removal-from-filtrate-of-digested-sludge
#2
Yury Nikolaev, Anna Kallistova, Marina Kevbrina, Alexander Dorofeev, Anton Agarev, Andrey Mardanov, Nikolay Ravin, Michail Kozlov, Nikolay Pimenov
Although anammox process is extensively applied to the treatment of high-strength ammonium wastewater, new technical solutions overcoming the operational difficulties remain an important task. In this study, an innovative design of anammox-based setup was employed to improve sludge settling under high ammonium load (up to 1000 mg NH4-N/l/d). The setup treated the filtrate of digested sludge and included a completely mixed constant flow bioreactor with suspended and immobilised activated sludge. Suspended sludge was recycled via a settler...
March 17, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28298637/hybrid-magnetic-dna-directed-immobilisation-approach-for-efficient-protein-capture-and-detection-on-microfluidic-platforms
#3
Elaheh Esmaeili, Mohammad Adel Ghiass, Manouchehr Vossoughi, Masoud Soleimani
In this study, a hybrid magnetic-DNA directed immobilisation approach is presented to enhance protein capture and detection on a microfluidic platform. DNA-modified magnetic nanoparticles are added in a solution to capture fluorescently labelled immunocomplexes to be detected optically. A magnetic set-up composed of cubic permanent magnets and a microchannel was designed and implemented based on finite element analysis results to efficiently concentrate the nanoparticles only over a defined area of the microchannel as the sensing zone...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28288856/environmental-enrichment-ameliorates-chronic-immobilisation-stress-induced-spatial-learning-deficits-and-restores-the-expression-of-bdnf-vegf-gfap-and-glucocorticoid-receptors
#4
B M Shilpa, V Bhagya, G Harish, M M Srinivas Bharath, B S Shankaranarayana Rao
Severe and prolonged stress is the main environmental factor that precipitates depression, anxiety and cognitive dysfunctions. On the other hand, exposure to environmental enrichment (EE) has been shown to induce progressive plasticity in the brain and improve learning and memory in various neurological and psychiatric disorders. It is not known whether exposure to enriched environment could ameliorate chronic immobilisation stress-induced cognitive deficits and altered molecular markers. Hence, in the present study we aimed to evaluate the effect of enriched environment on chronic immobilisation stress (CIS) associated changes in spatial learning and memory, behavioural measures of anxiety, depression and molecular markers as well as structural alterations...
March 10, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28281773/b-cell-epitopes-of-african-horse-sickness-virus-serotype-4-recognised-by-immune-horse-sera
#5
Evans M Mathebula, Frederika E Faber, Wouter Van Wyngaardt, Antoinette Van Schalkwyk, Alri Pretorius, Jeanni Fehrsen
Identifying antigenic proteins and mapping their epitopes is important for the development of diagnostic reagents and recombinant vaccines. B-cell epitopes of African horse sickness virus (AHSV) have previously been mapped on VP2, VP5, VP7 and NS1, using mouse, rabbit and chicken monoclonal antibodies. A comprehensive study of the humoral immune response of five vaccinated horses to AHSV-4 antigenic peptides was undertaken. A fragmented-genome phage display library expressing a repertoire of AHSV-4 peptides spanning the entire genome was constructed...
February 24, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/28281770/tremors-in-white-rhinoceroses-i-ceratotherium-simum-i-during-etorphine-azaperone-immobilisation
#6
Stephanie S De Lange, Andrea Fuller, Anna Haw, Markus Hofmeyr, Peter Buss, Michele Miller, Leith C R Meyer
Little is known about the mechanisms causing tremors during immobilisation of rhinoceros and whether cardiorespiratory supportive interventions alter their intensity. Therefore, we set out to determine the possible mechanisms that lead to muscle tremors and ascertain whether cardiorespiratory supportive interventions affect tremor intensity. We studied tremors and physiological responses during etorphine-azaperone immobilisation in eight boma-held and 14 free-living white rhinoceroses. Repeated measures analysis of variance and a Friedman test were used to determine differences in variables over time and between interventions...
February 24, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28279428/nadroparin-or-fondaparinux-versus-no-thromboprophylaxis-in-patients-immobilised-in-a-below-knee-plaster-cast-protect-a-randomised-controlled-trial
#7
Marlieke M Bruntink, Yannick M E Groutars, Inger B Schipper, Roelf S Breederveld, Wim E Tuinebreijer, Robert J Derksen
BACKGROUND: The immobilisation of the lower leg is associated with deep vein thrombosis (DVT). However, thromboprophylaxis in patients with a below-knee plaster cast remains controversial. We examined the efficacy and safety of nadroparin and fondaparinux to ascertain the need for thromboprophylaxis in these patients. METHODS: PROTECT was a randomised, controlled, single-blind, multicentre study that enrolled adults with an ankle or foot fracture who required immobilisation for a minimum of four weeks...
February 22, 2017: Injury
https://www.readbyqxmd.com/read/28279266/parallels-between-astronauts-and-terrestrial-patients-taking-physiotherapy-rehabilitation-to-infinity-and-beyond
#8
Julie Hides, Gunda Lambrecht, Gita Ramdharry, Rebecca Cusack, Jacob Bloomberg, Maria Stokes
Exposure to the microgravity environment induces physiological changes in the cardiovascular, musculoskeletal and sensorimotor systems in healthy astronauts. As space agencies prepare for extended duration missions, it is difficult to predict the extent of the effects that prolonged exposure to microgravity will have on astronauts. Prolonged bed rest is a model used by space agencies to simulate the effects of spaceflight on the human body, and bed rest studies have provided some insights into the effects of immobilisation and inactivity...
January 2017: Musculoskelet Sci Pract
https://www.readbyqxmd.com/read/28270985/nanoparticle-functionalised-small-core-suspended-core-fibre-a-novel-platform-for-efficient-sensing
#9
Brenda Doherty, Andrea Csáki, Matthias Thiele, Matthias Zeisberger, Anka Schwuchow, Jens Kobelke, Wolfgang Fritzsche, Markus A Schmidt
Detecting small quantities of specific target molecules is of major importance within bioanalytics for efficient disease diagnostics. One promising sensing approach relies on combining plasmonically-active waveguides with microfluidics yielding an easy-to-use sensing platform. Here we introduce suspended-core fibres containing immobilised plasmonic nanoparticles surrounding the guiding core as a concept for an entirely integrated optofluidic platform for efficient refractive index sensing. Due to the extremely small optical core and the large adjacent microfluidic channels, over two orders of magnitude of nanoparticle coverage densities have been accessed with millimetre-long sample lengths showing refractive index sensitivities of 170 nm/RIU for aqueous analytes where the fibre interior is functionalised by gold nanospheres...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28265461/a-protocol-for-a-feasibility-randomised-controlled-trial-to-assess-the-difference-between-functional-bracing-and-plaster-cast-for-the-treatment-of-ankle-fractures
#10
Rebecca S Kearney, Nick Parsons, Dipesh Mistry, Jonathan Young, Jaclyn Brown, Joanne O'Beirne-Elliman, Matthew Costa
BACKGROUND: UK Hospital Episode Statistics 2013-2014 recorded 57,286 fractures of the lower limb including the ankle. This figure is expected to continue to increase due to a greater population of older adults. Following an ankle fracture, patients usually have their ankle immobilised with a plaster cast. This provides maximum support for the healing ankle but is associated with stiffness and muscle wasting. A Cochrane Review has concluded that functional bracing may reduce muscle wasting and speed recovery of ankle movement...
2017: Pilot and Feasibility Studies
https://www.readbyqxmd.com/read/28264511/prevention-of-bacterial-contamination-of-a-silica-matrix-containing-entrapped-%C3%AE-galactosidase-through-the-action-of-covalently-bound-lysozymes
#11
Heng Li, Shuai Li, Pu Tian, Zhuofu Wu, Zhengqiang Li
β-galactosidase was successfully encapsulated within an amino-functionalised silica matrix using a "fish-in-net" approach and molecular imprinting technique followed by covalent binding of lysozyme via a glutaraldehyde-based method. Transmission electron microscopy (TEM), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy were used to characterise the silica matrix hosting the two enzymes. Both encapsulated β-galactosidase and bound lysozyme exhibited high enzymatic activities and outstanding operational stability in model reactions...
February 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28254426/dna-damage-in-lymphocytes-of-patients-suffering-from-complex-traumatization
#12
Liana M Bergholz, Claudia Subic-Wrana, Daniel Heylmann, Manfred E Beutel, Jörg Wiltink, Bernd Kaina
It has been shown that complex childhood traumatization (CCT) increases the risk for severe somatic and mental disorders. However, the somatic pathways linking maladaptive affect regulation and disease are not understood. Here we tested the hypothesis that traumatic stress is linked to the induction of DNA damage. We isolated peripheral lymphocytes (PBLCs) from blood of healthy donors and patients suffering from CCT. Cells were immobilised on slides and stained with anti-phosphohistone 2AX (γH2AX). The number of γH2AX foci, which are an accepted surrogate marker of DNA double-strand breaks (DSBs), was determined and set in relation to the patient characteristics...
February 9, 2017: DNA Repair
https://www.readbyqxmd.com/read/28252668/aggretin-venom-polypeptide-as-a-novel-anti-angiogenesis-agent-by-targeting-integrin-alpha2beta1
#13
Ching Hu Chung, Chien Hsin Chang, Chun Chieh Hsu, Kung Tin Lin, Hui Chin Peng, Tur Fu Huang
VEGF and VEGFR antibodies have been used as a therapeutic strategy to inhibit angiogenesis in many diseases; however, frequent and repeated administration of these antibodies to patients induces immunogenicity. In previous studies, we demonstrated that aggretin, a heterodimeric snake venom C-type lectin, exhibits pro-angiogenic activities via integrin α2β1 ligation. We hypothesised that small-mass aggretin fragments may bind integrin α2β1 and act as antagonists of angiogenesis. In this study, the anti-angiogenic efficacy of a synthesised aggretin α-chain C-terminus (AACT, residue 106-136) was evaluated in both in vitro and in vivo angiogenesis models...
March 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28251068/lower-limb-intracast-pressures-generated-by-different-types-of-immobilisation-casts
#14
Salma Chaudhury, Alexandra Hazlerigg, Anuhya Vusirikala, Joseph Nguyen, Stuart Matthews
AIM: To determine if complete, split casts and backslabs [plaster of Paris (POP) and fiberglass] generate different intracast pressures and pain. METHODS: Increased swelling within casts was modeled by a closed water system attached to an expandable bag placed directly under different types of casts applied to a healthy lower limb. Complete fiberglass and POP casts, split casts and backslabs were applied. Twenty-five milliliter aliquots of saline were injected into the system and the generated intracast pressures were measured using a sphygmomanometer...
February 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/28248633/evaluation-of-smartflare-probe-applicability-for-verification-of-rnas-in-early-equine-conceptuses-equine-dermal-fibroblast-cells-and-trophoblastic-vesicles
#15
S Budik, W Tschulenk, S Kummer, I Walter, C Aurich
Live cell RNA imaging has become an important tool for studying RNA localisation, dynamics and regulation in cultured cells. Limited information is available using these methods in more complex biological systems, such as conceptuses at different developmental stages. So far most of the approaches rely on microinjection of synthetic constructs into oocytes during or before fertilisation. Recently, a new generation of RNA-specific probes has been developed, the so named SmartFlare probes (Merck Millipore). These consist of a central 15-nm gold particle with target-specific DNAs immobilised on its surface...
March 2, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28243661/reversible-dna-micro-patterning-using-the-fluorous-effect
#16
Gabriella E Flynn, Jamie M Withers, Gerard Macias, Justin R Sperling, Sarah L Henry, Jonathan M Cooper, Glenn A Burley, Alasdair W Clark
We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.
February 28, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28242376/cell-based-therapeutics-in-type-1-diabetes-mellitus
#17
Fernanda Zamboni, Maurice N Collins
This review focuses on Type 1 diabetes mellitus (T1DM) and the role of bioengineering, nanotechnology and cell therapy in its treatment. T1DM is discussed in terms of its prevalence as well as the role of the extra cellular matrix (ECM) of the pancreas in its development and mode of action. Surface engineering strategies and the chemistries behind important cell encapsulation techniques, which are emerging from recent research in immunosuppression, are described. Key enabling technologies such as therapeutic agent immobilization on cells, oxygen releasing systems, gene delivery and bio imaging are assessed with respect to T1DM...
February 27, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28240902/organic-cation-rotation-and-immobilization-in-pure-and-mixed-methylammonium-lead-halide-perovskites
#18
Oleg Selig, Aditya Sadhanala, Christian Müller, Robert Lovrincic, Zhuoying Chen, Yves L A Rezus, Jarvist M Frost, Thomas L C Jansen, Artem A Bakulin
Three-dimensional lead-halide perovskites have attracted a lot of attention due to their ability to combine solution processing with outstanding optoelectronic properties. Despite their soft ionic nature these materials demonstrate a surprisingly low level of electronic disorder resulting in sharp band edges and narrow distributions of the electronic energies. Understanding how structural and dynamic disorder impacts the optoelectronic properties of these perovskites is important for many applications. Here we combine ultrafast two-dimensional vibrational spectroscopy and molecular dynamics simulations to study the dynamics of the organic methylammonium (MA) cation orientation in a range of pure and mixed trihalide perovskite materials...
March 9, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28230769/hypoallergenic-variant-of-the-major-egg-white-allergen-gal-d-1-produced-by-disruption-of-cysteine-bridges
#19
Pathum Dhanapala, Dulashi Withanage-Dona, Mimi L K Tang, Tim Doran, Cenk Suphioglu
Gal d 1 (ovomucoid) is the dominant allergen in the chicken egg white. Hypoallergenic variants of this allergen can be used in immunotherapy as an egg allergy treatment approach. We hypothesised that disruption of two of the nine cysteine-cysteine bridges by site-directed mutagenesis will allow the production of a hypoallergenic variant of the protein; Methods: Two cysteine residues at C192 and C210 in domain III of the protein were mutated to alanine using site-directed mutagenesis, to disrupt two separate cysteine-cysteine bridges...
February 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28217778/a-critical-insight-into-the-development-pipeline-of-microfluidic-immunoassay-devices-for-the-sensitive-quantitation-of-protein-biomarkers-at-the-point-of-care
#20
REVIEW
Ana I Barbosa, Nuno M Reis
The latest clinical procedures for the timely and cost-effective diagnosis of chronic and acute clinical conditions, such as cardiovascular diseases, cancer, chronic respiratory diseases, diabetes or sepsis (i.e. the biggest causes of death worldwide), involve the quantitation of specific protein biomarkers released into the blood stream or other physiological fluids (e.g. urine or saliva). The clinical thresholds are usually in the femtomolar to picolomar range, and consequently the measurement of these protein biomarkers heavily relies on highly sophisticated, bulky and automated equipment in centralised pathology laboratories...
March 13, 2017: Analyst
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