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https://www.readbyqxmd.com/read/28648471/optimisation-of-continuous-gas-fermentation-by-immobilisation-of-acetate-producing-acetobacterium-woodii
#1
Franziska Steger, Lydia Rachbauer, Matthias Windhagauer, Lucy F R Montgomery, Günther Bochmann
Hydrogen from water electrolysis is often suggested as a way of storing the excess energy from wind and solar power plants. However, unlike natural gas, hydrogen is difficult to store and distribute. One solution is to convert the hydrogen into other fuels or bulk chemicals. In this study we investigated fermentation in which homoacetogenic clostridia apply the Wood-Ljungdahl pathway to generate acetate from H2 and CO2. Acetate can be used as a bulk chemical or further transformed into biofuels. Autotrophic growth with CO2 as the sole carbon source is slow compared to heterotrophic growth, so the aim of this work was to improve continuous gas fermentation by immobilising the acetate-producing clostridia, thus preventing their wash out from the bioreactor...
June 22, 2017: Anaerobe
https://www.readbyqxmd.com/read/28645976/a-combined-randomised-and-observational-study-of-surgery-for-fractures-in-the-distal-radius-in-the-elderly-crossfire-a-study-protocol
#2
Ian A Harris, Justine M Naylor, Andrew Lawson, Rachelle Buchbinder, Rebecca Ivers, Zsolt Balogh, Paul Smith, Rajat Mittal, Wei Xuan, Kirsten Howard, Arezoo Vafa, Piers Yates, Bertram Rieger, Geoff Smith, Ilia Elkinson, Woosung Kim, Mellick Chehade, Jai Sungaran, Kim Latendresse, James Wong, Sameer Viswanathan, Martin Richardson, Kush Shrestha, Herwig Drobetz, Phong Tran, Jeremy Loveridge, Richard Page, Raphael Hau, Roger Bingham, Jonathan Mulford, Ian Incoll
Fractures of the distal radius are common and occur in all age groups. The incidence is high in older populations due to osteoporosis and increased falls risk. Considerable practice variation exists in the management of distal radius fractures in older patients ranging from closed reduction with cast immobilisation to open reduction with plate fixation. Plating is currently the most common surgical treatment. While there is evidence showing no significant advantage for some forms of surgical fixation over conservative treatment, and no difference between different surgical techniques, there is a lack of evidence comparing two of the most common treatments used: closed reduction and casting versus plating...
June 23, 2017: BMJ Open
https://www.readbyqxmd.com/read/28635414/core-shell-pla-pva-porous-microparticles-as-carriers-for-bacteriocin-nisin
#3
Pavlina Holcapkova, Martina Hrabalikova, Petra Stoplova, Vladimir Sedlarik
This work is focused on preparation of novel porous type of core-shell-structured microparticles based on polylactide (shell) and poly(vinyl alcohol) cross-linked with glutaric acid (GA) (core) prepared by water-in-oil-in-water solvent evaporation technique. The microparticle systems were used as delivery systems for immobilisation of model antibacterial agent - nisin. The effect of cross-linking and the initial amount of nisin on their morphology was investigated using scanning electron microscopy, BET analysis, zeta potential measurement and Fourier transform infra-red spectroscopy...
June 21, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28633064/an-innovative-approach-to-bioremediation-of-mercury-contaminated-soils-from-industrial-mining-operations
#4
Damien McCarthy, Grant C Edwards, Mae S Gustin, Andrew Care, Matthieu B Miller, Anwar Sunna
Soils contaminated with mercury (Hg) have proved expensive and logistically difficult to remediate. Research continues into finding suitable environmentally-friendly and efficient ways of achieving this end. Bioremediation is an option, which employs the strategies microorganisms have evolved to deal with Hg. One microbial strategy involves uptake and intracellular volatilisation of mercuric ions, which passively diffuse from the cell and back into the atmosphere. In this work, Pseudomonas veronii cells grown to stationary phase were immobilised in a xanthan gum-based biopolymer via encapsulation...
June 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28628902/recycling-stabilised-solidified-drill-cuttings-for-forage-production-in-acidic-soils
#5
Reginald B Kogbara, Bernard B Dumkhana, Josiah M Ayotamuno, Reuben N Okparanma
Stabilisation/solidification (S/S), which involves fixation and immobilisation of contaminants using cementitious materials, is one method of treating drill cuttings before final fate. This work considers reuse of stabilised/solidified drill cuttings for forage production in acidic soils. It sought to improve the sustainability of S/S technique through supplementation with the phytoremediation potential of plants, eliminate the need for landfill disposal and reduce soil acidity for better plant growth. Drill cuttings with an initial total petroleum hydrocarbon (TPH) concentration of 17,125 mg kg(-1) and low concentrations of metals were treated with 5%, 10%, and 20% cement dosages...
June 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28622601/immobilisation-of-barley-aleurone-layers-enables-parallelisation-of-assays-and-analysis-of-transient-gene-expression-in-single-cells
#6
Kinga Zór, Christina Mark, Arto Heiskanen, Claus Krogh Madsen, Martin Dufva, Jenny Emnéus, Henrik Brinch-Pedersen, Christine Finnie
The barley aleurone layer is an established model system for studying phytohormone signalling, enzyme secretion and programmed cell death during seed germination. Most analyses performed on the aleurone layer are end-point assays based on cell extracts, meaning each sample is only analysed at a single time point. By immobilising barley aleurone layer tissue on polydimethylsiloxane pillars in the lid of a multiwell plate, continuous monitoring of living tissue is enabled using multiple non-destructive assays in parallel...
June 8, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28613326/arbuscular-mycorrhizal-fungi-enhance-the-copper-tolerance-of-tagetes-patula-through-the-sorption-and-barrier-mechanisms-of-intraradical-hyphae
#7
Xishi Zhou, Lei Fu, Yan Xia, Luqing Zheng, Chen Chen, Zhenguo Shen, Yahua Chen
Arbuscular mycorrhizal fungi (AMF) are widespread soil fungi that can form endosymbiotic structures with the root systems of most plants and can improve the tolerance of host plants to heavy metals. In the present study, we investigated the effects of AMF (Glomus coronatum) inoculation on the tolerance of Tagetes patula L. to Cu. Almost all of the non-mycorrhizal plants exposed to 100 μM Cu died after 3 d, whereas phytotoxicity was only observed in mycorrhizal plants that were exposed to Cu concentrations greater than 100 μM...
June 14, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28608009/clinical-outcomes-of-extra-articular-tenodesis-anterolateral-reconstruction-in-the-acl-injured-knee
#8
REVIEW
Bertrand Sonnery-Cottet, Nuno Camelo Barbosa, Thais Dutra Vieira, Adnan Saithna
PURPOSE: The role of concomitant extra-articular procedures in improving the outcome of ACL reconstruction has experienced a recent resurgence in interest. The aim of this article is to highlight the differences in philosophies and outcomes of historical lateral extra-articular tenodesis (LET) and contemporary, anterolateral reconstruction. METHODS: A narrative review was performed using PubMed/MEDLINE using the keywords "lateral extra-articular tenodesis" and "anterolateral ligament reconstruction"...
June 12, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28601436/evaluating-trace-element-bioavailability-and-potential-transfer-into-marine-food-chains-using-immobilised-diatom-model-species-phaeodactylum-tricornutum-on-king-george-island-antarctica
#9
Maria Teresa Cabrita, Ana Padeiro, Eduardo Amaro, Margarida Correia Dos Santos, Marcelo Leppe, Sergey Verkulich, Kevin A Hughes, Hans-Ulrich Peter, João Canário
In order to evaluate trace element bioavailability and potential transfer into marine food chains in human impacted areas of the Fildes Peninsula (King George Island, South Shetland Islands Archipelago), element levels (Cr, Ni, Cu, Zn, Cd, and Pb) were determined in water, sediments, phytoplankton, and in diatom Phaeodactylum tricornutum Bohlin (Bacillariophyceae) cells immobilised in alginate and exposed to water and sediments, from the Bellingshausen Dome (reference site) and Ardley Cove (human impacted area), during January 2014...
June 7, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28599860/molecularly-imprinted-nanoparticles-grafted-to-porous-silica-as-chiral-selectors-in-liquid-chromatography
#10
Raquel Gutierrez-Climente, Alberto Gomez-Caballero, Antonio Guerreiro, Deiene Garcia-Mutio, Nora Unceta, M Aránzazu Goicolea, Ramon J Barrio
The work presented here explores the grafting of molecularly imprinted nanoparticles (MIN) on silica beads for the development of new chiral stationary phases (CSP). Both solid-phase imprinting and precipitation polymerisation were tested for MIN synthesis though the latter approach was the only one that provided efficient chiral selectors. MIN particles were prepared by iniferter polymerisation initiated by UV radiation, using itaconic acid as functional monomer and ethylene dimethacrylate as cross-linker...
June 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28587287/a-routine-top-down-approach-to-analysis-of-the-human-serum-proteome
#11
Arlene M D'Silva, Jon A Hyett, Jens R Coorssen
Serum provides a rich source of potential biomarker proteoforms. One of the major obstacles in analysing serum proteomes is detecting lower abundance proteins owing to the presence of hyper-abundant species (e.g., serum albumin and immunoglobulins). Although depletion methods have been used to address this, these can lead to the concomitant removal of non-targeted protein species, and thus raise issues of specificity, reproducibility, and the capacity for meaningful quantitative analyses. Altering the native stoichiometry of the proteome components may thus yield a more complex series of issues than dealing directly with the inherent complexity of the sample...
June 6, 2017: Proteomes
https://www.readbyqxmd.com/read/28582708/new-immobilisation-method-for-oligonucleotides-on-electrodes-enables-highly-sensitive-electrochemical-label-free-gene-sensing
#12
Nihan Aydemir, Eddie Chan, Paul Baek, David Barker, David E Williams, Jadranka Travas-Sejdic
We present a versatile and facile procedure for the immobilisation of bioprobe molecules to an electrochemical sensing element. We eliminate lengthy preparation procedures for direct functionalisation of electrode surfaces by pre-attaching probe molecules to carboxylic acid bearing termonomers of pyrrole phenylenes or thiophene phenylenes. We demonstrate that these conjugates can be electrodeposited at low potentials to form nano-scale porous, electroactive conducting polymer films, exposing the bioprobe and retaining activity and specificity for binding, exemplified here with DNA sensors...
June 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28574700/a-facile-way-of-modifying-layered-double-hydroxide-nanoparticles-with-targeting-ligand-conjugated-albumin-for-enhanced-delivery-to-brain-tumour-cells
#13
Huali Zuo, Weiyu Chen, Helen M Cooper, Zhi Ping Xu
Active targeting of nanoparticles (NPs) for cancer treatment has attracted increasing interest in the past decades. Various ligand modification strategies have been used to enhance the targeting of NPs to the tumor site. However, how to reproducibly fabricate diverse targeting NPs with narrowly changeable biophysiochemical properties remains as a major challenge. In this study, layered double hydroxide (LDH) NPs were modified as a target delivery system. Two brain tumor targeting ligands, i.e., angiopep-2 and rabies virus glycoprotein, were conjugated to the LDH NPs via an intermatrix protein moiety, bovine serum albumin (BSA), simultaneously endowing the LDHs with excellent colloidal stability and targeting capability...
June 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28570937/recent-advancements-in-supporting-materials-for-immobilised-photocatalytic-applications-in-waste-water-treatment
#14
REVIEW
B Srikanth, R Goutham, R Badri Narayan, A Ramprasath, K P Gopinath, A R Sankaranarayanan
The aim of this paper is to provide a review on the usage of different anchoring media (supports) for immobilising commonly employed photocatalysts for degradation of organic pollutants. The immobilisation of nano-sized photocatalysts can eliminate costly and impractical post-treatment recovery of spent photocatalysts in largescale operations. Some commonly employed immobilisation aids such as glass, carbonaceous substances, zeolites, clay and ceramics, polymers, cellulosic materials and metallic agents that have been previously discussed by various research groups have been reviewed...
May 29, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28567158/plaster-of-paris-short-history-of-casting-and-injured-limb-immobilzation
#15
REVIEW
B Szostakowski, P Smitham, W S Khan
Various materials have been used since ancient times to help immobilise fractures. In this review, we discuss the history and developments of these materials as well as plaster of Paris. There has been a recent trend away from non-operative management of fractures, and skills in the use of plaster of Paris are declining. For the successful treatment of patients, it is important to appreciate how plaster works, how it should be used, and what can go wrong. In this review, we also discuss principles of applications and complications of plaster of Paris...
2017: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/28558204/development-and-application-of-synthetic-affinity-ligands-for-the-purification-of-ferritin-based-influenza-antigens
#16
Shaleem I Jacob, Basmah Khogeer, Nick Bampos, Tom Sheppard, Richard Schwartz, Christopher R Lowe
A recently developed novel recombinant influenza antigen vaccine has shown great success in preclinical studies in ferrets and mice. It provides broader protection, and is efficient to manufacture compared to the conventional trivalent influenza vaccines (TIV). Each strain of the recombinant antigen has a constant self-assembled bacterial ferritin core which, if used as a target for affinity chromatography, could lead to a universal purification method. Ferritin in silico models were used to explore potential target binding sites against ligands synthesized by the four-component Ugi reaction...
June 21, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28556850/clinical-profile-management-and-outcomes-of-patients-with-pulmonary-embolism-a-retrospective-tertiary-centre-study-in-angola
#17
Ana Manuel, Adelina Aufico, Rui Africano, Tomáz Peralta, Abel Salas, Adelaide Silva, José Ricardo, Pedro Sabola, Domingas Baião, Carlos Sotolongo, António Dias Neto, Telmo Martins, Vasco Sabino, Joaquim van Dúnem, António Pedro Filipe Júnior
OBJECTIVE: Pulmonary embolism (PE) is a potentially fatal disease. In Angola, few data are available on its occurrence. The aim of the study was to characterise the clinical profile, management and outcomes of patients with PE. METHODS: A retrospective observational study was conducted at the Girassol Clinic in Luanda, Angola. The medical records of patients admitted to the intensive care unit were analysed from 2011 to 2015. RESULTS: Fifty patients were included and the median age was 50...
May 17, 2017: Cardiovascular Journal of Africa
https://www.readbyqxmd.com/read/28554045/transparent-mediator-and-membrane-free-enzymatic-fuel-cell-based-on-nanostructured-chemically-modified-indium-tin-oxide-electrodes
#18
Elena González-Arribas, Tim Bobrowski, Chiara Di Bari, Kirill Sliozberg, Roland Ludwig, Miguel D Toscano, Antonio L De Lacey, Marcos Pita, Wolfgang Schuhmann, Sergey Shleev
We detail a mediator- and membrane-free enzymatic glucose/oxygen biofuel cell based on transparent and nanostructured conducting supports. Chemically modified indium tin oxide nanoparticle modified electrodes were used to substantially increase the active surface area without significantly compromising transparency. Two different procedures for surface nanostructuring were employed, viz. spray-coating and drop-coating. The spray-coated biodevice showed superior characteristics as compared to the drop-coated enzymatic fuel cell, as a result of the higher nanostructured surface area as confirmed by electrochemical characterisation, as well as scanning electron and atomic force microscopy...
May 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28553442/physical-and-clinical-evaluation-of-hip-spica-cast-applied-with-three-slab-technique-using-fibreglass-material
#19
K M Bitar, M E Ferdhany, E I Ashraf, A Saw
Introduction: Hip spica casting is an important component of treatment for developmental dysplasia of the hip (DDH) and popular treatment method for femur fractures in children. Breakage at the hip region is a relatively common problem of this cast. We have developed a three-slab technique of hip spica application using fibreglass as the cast material. The purpose of this review was to evaluate the physical durability of the spica cast and skin complications with its use. Methodology: A retrospective review of children with various conditions requiring hip spica immobilisation which was applied using our method...
November 2016: Malaysian Orthopaedic Journal
https://www.readbyqxmd.com/read/28551538/daphnia-swimming-behaviour-as-a-biomarker-in-toxicity-assessment-a-review
#20
REVIEW
Adam Bownik
Daphnia is a motile common model organism widely used in ecotoxicological testing. Although mortality and immobilisation are the main endpoints used for determination of toxicity, detection of subtle alterations induced by some chemicals particularly at lower levels may require more sensitive biomarkers. As a number of studies indicated that swimming behaviour may be altered by pesticides, nanoparticles, bacterial products or other chemicals, analysis of its various parameters is considered as a novel methodological approach for toxicity assessment and monitoring of water quality...
May 25, 2017: Science of the Total Environment
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