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https://www.readbyqxmd.com/read/28723215/discrimination-cascade-enabled-selective-detection-of-single-nucleotide-mutation
#1
Lidan Li, Xianjin Xiao, Jingyang Ge, Manli Han, Xu Zhou, Lei Wang, Xin Su, Changyuan Yu
Owing to the significance of single nucleotide mutation (SNM) for personalized medicine, the detection of SNM with high accuracy has recently attracted considerable interest. Here, we present a kinetic method for selective detection of SNM based on a discrimination cascade constructed by combining the toehold strand displacement (TSD) and endonuclease IV (Endo IV) catalyzed hydrolysis. The single-nucleotide specificity of the two DNA reactions allows highly selective detection of all types of single nucleotide changes (including single-nucleotide insertion and deletion), achieving a high discrimination factor with a median of 491 which is comparable with recently reported methods...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28720149/heparanase-promotes-neuroinflammatory-response-during-subarachnoid-hemorrhage-in-rats
#2
Benjarat Changyaleket, Zhao Zhong Chong, Randal O Dull, Danop Nanegrungsunk, Haoliang Xu
BACKGROUND: Heparanase, a mammalian endo-β-D-glucoronidase that specifically degrades heparan sulfate, has been implicated in inflammation and ischemic stroke. However, the role of heparanase in neuroinflammatory response in subarachnoid hemorrhage (SAH) has not yet been investigated. This study was designed to examine the association between heparanase expression and neuroinflammation during subarachnoid hemorrhage. METHODS: Rats were subjected to SAH by endovascular perforation, and the expression of heparanase was determined by Western blot analysis and immunofluorescence in the ipsilateral brain cortex at 24 h post-SAH...
July 18, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28708780/syndesmotic-ankle-fractures-a-systematic-review
#3
James D Michelson, Michael Wright, Michael Blankstein
OBJECTIVES: The diagnosis and treatment of syndesmotic ankle fractures is controversial. This systematic review was performed to clarify the current understanding addressing several clinical questions that arise in the treatment of such injuries DATA SOURCES:: The English language literature was searched using PubMed, EMBASE, and Web of Science. The search terms were "syndesmosis" or "syndesmotic" in combination with the terms "ankle fracture" or "fracture". The included dates were between 1967 and July 2015...
June 22, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28700083/torsional-resistance-of-xp-endo-shaper-at-body-temperature-compared-with-several-nickel-titanium-rotary-instruments
#4
A M Elnaghy, S E Elsaka
AIM: To compare the torsional resistance of XP-endo Shaper (XPS; size 30, 0.01 taper, FKG Dentaire, La Chaux-de-Fonds, Switzerland) instruments at body temperature with TRUShape (TRS; size 30, 0.06 taper, Dentsply Tulsa Dental Specialties, Tulsa, OK, USA), ProFile Vortex (PV; size 30, 0.04 taper, Dentsply Tulsa Dental Specialties), and FlexMaster (FM; size 30, 0.04 taper, VDW GmbH, Munich, Germany) nickel-titanium rotary instruments. METHODOLOGY: A metal block with a square-shaped mould (5 mm × 5 mm × 5 mm) was positioned inside a glass-container...
July 12, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28669632/regulation-of-the-equilibrium-between-closed-and-open-conformations-of-annexin-a2-by-n-terminal-phosphorylation-and-s100a4-binding
#5
Péter Ecsédi, Bence Kiss, Gergő Gógl, László Radnai, László Buday, Kitti Koprivanacz, Károly Liliom, Ibolya Leveles, Beáta Vértessy, Norbert Jeszenői, Csaba Hetényi, Gitta Schlosser, Gergely Katona, László Nyitray
Annexin A2 (ANXA2) has a versatile role in membrane-associated functions including membrane aggregation, endo- and exocytosis, and it is regulated by post-translational modifications and protein-protein interactions through the unstructured N-terminal domain (NTD). Our sequence analysis revealed a short motif responsible for clamping the NTD to the C-terminal core domain (CTD). Structural studies indicated that the flexibility of the NTD and CTD are interrelated and oppositely regulated by Tyr24 phosphorylation and Ser26Glu phosphomimicking mutation...
June 21, 2017: Structure
https://www.readbyqxmd.com/read/28645598/efficacy-of-early-surgery-for-neurological-improvement-in-spinal-cord-injury-without-radiographic-evidence-of-trauma-in-the-elderly
#6
Tomoo Inoue, Shinsuke Suzuki, Toshiki Endo, Hiroshi Uenohara, Teiji Tominaga
OBJECT: The optimal timing for surgery for patients with spinal cord injury without radiographic evidence of trauma (SCIWORET) remains unclear. This is especially true in the elderly, because most studies are done with younger patients to avoid age-related comorbidities. We therefore aimed to compare the efficacy of early (less than 24 hours post-injury) versus late (more than 24 hours post-injury) surgery in patients with SCIWORET aged 65 years or older. METHODS: We identified patients aged 65 years or older who underwent surgery for SCIWORET between January 1995 and February 2016...
June 20, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28643130/retreatability-of-three-calcium-silicate-containing-sealers-and-one-epoxy-resin-based-root-canal-sealer-with-four-different-root-canal-instruments
#7
David Donnermeyer, Clarissa Bunne, Edgar Schäfer, Till Dammaschke
OBJECTIVES: The objective of the study was to compare the retreatability of three calcium silicate-containing sealers (BioRoot RCS, MTA Fillapex, Endo C.P.M.) and an epoxy resin-based sealer (AH Plus) with different root canal instruments (Hedström files, Reciproc R40, Mtwo retreatment file R 25/.05 + Mtwo 40/.06, and F6 SkyTaper) concerning sealer remnants and retreatment time. MATERIALS AND METHODS: Root canals of 192 teeth were instrumented with Reciproc R40...
June 22, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28596270/timing-of-post-exercise-carbohydrate-protein-supplementation-roles-of-gastrointestinal-blood-flow-and-mucosal-cell-damage-on-gastric-emptying-in-humans
#8
Hideaki Kashima, Nao Harada, Kanae Miyamoto, Masaki Fujimoto, Chiaki Fujita, Masako Yamaoka Endo, Toshio Kobayashi, Akira Miura, Yoshiyuki Fukuba
It is well known that protein ingestion immediately after exercise greatly stimulates muscle protein synthesis during the post-exercise recovery phase. However, immediately after strenuous exercise, the gastrointestinal (GI) mucosa is frequently injured by hypoperfusion in the organ/tissue, possibly resulting in impaired GI function (e.g., gastric emptying; GE). The aim of this study was to examine the effect of GI blood flow on the GE rate. Eight healthy young subjects performed an intermittent supramaximal cycling exercise for 30 min, which consisted of a 120% VO2 peak for 20 s, followed by 20 W for 40 s...
June 8, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28591073/predictors-of-persistent-axial-neck-pain-after-cervical-laminoplasty
#9
Atsushi Kimura, Yasuyuki Shiraishi, Hirokazu Inoue, Teruaki Endo, Katsushi Takeshita
STUDY DESIGN: Retrospective analysis of prospective data OBJECTIVE.: The aim of this study was to reveal baseline predictors of persistent post-laminoplasty neck pain. SUMMARY OF BACKGROUND DATA: Axial neck pain is one of the most common complications after cervical laminoplasty; however, baseline predictors of persistent post-laminoplasty neck pain are unclear. METHODS: We analyzed data from 156 patients who completed a 2-year follow-up after double-door laminoplasty for degenerative cervical myelopathy (DCM)...
June 6, 2017: Spine
https://www.readbyqxmd.com/read/28556923/antiarrhythmic-properties-of-ivabradine-in-an-experimental-model-of-short-qt-syndrome
#10
Gerrit Frommeyer, Jan Weller, Christian Ellermann, Sven Kaese, Simon Kochhäuser, Philipp S Lange, Dirk G Dechering, Lars Eckardt
The If channel inhibitor ivabradine is recommended for treatment of chronic heart failure. However, ivabradine also inhibits human ether-a-go-go (hERG) mediated potassium currents. The aim of the present study was to assess the electrophysiologic effects of ivabradine in an experimental model of short-QT-syndrome. 12 rabbit hearts were isolated and Langendorff-perfused. After obtaining baseline data, pinacidil, an IK-ATP channel opener, was infused (1μM). Eight endo- and epicardial monophasic action potentials and a 12-lead ECG showed a significant abbreviation of QT interval (-32ms,p<0...
May 29, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28526572/comprehensive-evaluation-of-immunomodulation-by-moderate-hypoxia-in-s-%C3%A2-agalactiae-vaccinated-nile-tilapia
#11
Sanchala Gallage, Takayuki Katagiri, Masato Endo, Masashi Maita
Streptococcus agalactiae is a major bacterial pathogen in tilapia aquaculture. Vaccines are known to provide protection but S. agalactiae clearance in tilapia can be reduced by marginal environmental conditions. Therefore, the purpose of this study is to examine S. agalactiae clearance in vaccinated Nile tilapia under moderate hypoxic (55± 5% DO) and normoxic (85 ± 5%DO) conditions. Fish were acclimatized to either moderate hypoxia or normoxia and immunized with formalin-inactivated S. agalactiae. Fish were experimentally challenged with S...
May 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28512494/cleanliness-of-canal-walls-following-gutta-percha-removal-with-hand-files-race-and-race-plus-xp-endo-finisher-instruments-a-photographic-in-vitro-analysis
#12
Kasra Karamifar, Neda Mehrasa, Pouyan Pardis, Mohammad Ali Saghiri
INTRODUCTION: Gutta-percha must be removed from the root canal space during retreatment to ensure a more favorable outcome. The aim of this study was to compare the efficacy of hand instruments, RaCe and RaCe plus XP-endo finisher instruments in removal of gutta-percha from root canal walls during retreatment. METHODS AND MATERIALS: Thirty single-rooted premolars were prepared, obturated, and divided into three groups according to retreatment method; in group 1, retreatment was carried out by hand instruments, while in groups 2 and 3 retreatment was done using RaCe rotary files alone or accompanied by XP-endo finisher instruments, respectively...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28503479/cyclic-fatigue-life-of-tango-endo-waveone-gold-and-reciproc-niti-instruments
#13
Koray Yılmaz, Taha Özyürek
OBJECTIVES: To compare the fatigue life of Tango-Endo, WaveOne GOLD, and Reciproc NiTi instruments under static model via artificial canals with different angles of curvature. MATERIALS AND METHODS: Reciproc R25, WaveOne GOLD Primary, and Tango-Endo instruments were included in this study (n = 20). All the instruments were rotated in artificial canals which were made of stainless steel with an inner diameter of 1.5 mm, 45°, 60°, and 90° angles of curvatures and a radius of curvature of 5 mm until fracture occurred, and the time to fracture was recorded in seconds using a digital chronometer...
May 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28494867/essential-roles-of-satb1-in-specifying-t-lymphocyte-subsets
#14
Kiyokazu Kakugawa, Satoshi Kojo, Hirokazu Tanaka, Wooseok Seo, Takaho A Endo, Yohko Kitagawa, Sawako Muroi, Mari Tenno, Nighat Yasmin, Yoshinori Kohwi, Shimon Sakaguchi, Terumi Kowhi-Shigematsu, Ichiro Taniuchi
T cell receptor (TCR) signaling by MHC class I and II induces thymocytes to acquire cytotoxic and helper fates via the induction of Runx3 and ThPOK transcription factors, respectively. The mechanisms by which TCR signaling is translated into transcriptional programs for each cell fate remain elusive. Here, we show that, in post-selection thymocytes, a genome organizer, SATB1, activates genes for lineage-specifying factors, including ThPOK, Runx3, CD4, CD8, and Treg factor Foxp3, via regulating enhancers in these genes in a locus-specific manner...
May 9, 2017: Cell Reports
https://www.readbyqxmd.com/read/28474756/disseminated-toxoplasmosis-after-hematopoietic-stem-cell-transplantation-showing-unusual-magnetic-resonance-images
#15
Takahiro Tateno, Masahiro Onozawa, Junichi Hashiguchi, Takashi Ishio, Sayaka Yuzawa, Satomi Matsuoka, Mizuha Kosugi-Kanaya, Kohei Okada, Souichi Shiratori, Hideki Goto, Taichi Kimura, Junichi Sugita, Masao Nakagawa, Daigo Hashimoto, Kaoru Kahata, Katsuya Fujimoto, Tomoyuki Endo, Takeshi Kondo, Shinya Tanaka, Satoshi Hashino, Takanori Teshima
We herein report a patient who had disseminated toxoplasmosis after hematopoietic stem cell transplantation showing atypical clinical presentation and neuroimaging. Parkinsonism symptoms such as muscle rigidity, bradykinesia, tremor, and postural instability were initial manifestations. Magnetic resonance imaging showed diffuse symmetrical lesions of bilateral basal ganglia lacking ringed enhancement. Post-mortem analysis revealed multiple tachyzoites of Toxoplasma gondii in the basal ganglia, mid brain, cerebellum, and cardiac muscle...
May 5, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28454634/vibrations-generated-by-several-nickel-titanium-endodontic-file-systems-during-canal-shaping-in%C3%A2-an-ex%C3%A2-vivo-model
#16
Dong-Min Choi, Jin-Woo Kim, Se-Hee Park, Kyung-Mo Cho, Sang Won Kwak, Hyeon-Cheol Kim
INTRODUCTION: This study aimed to compare the vibration generated by several nickel-titanium (NiTi) file systems and transmitted to teeth under 2 different motions (continuous rotation motion and reciprocating motion). METHODS: Sixty J-shaped resin blocks (Endo Training Bloc-J; Dentsply Maillefer, Ballaigues, Switzerland) were trimmed to a root-shaped form and divided into 2 groups according to the types of electric motors: WaveOne motor (WOM, Dentsply Maillefer) and X-Smart Plus motor (XSM, Dentsply Maillefer)...
April 25, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28422594/intentional-replantation-of-an-immature-incisor-with-a-transverse-root-fracture-and-endo-perio-condition-4-year-follow-up
#17
Atsushi Oishi
This case report describes the importance of continual examination for dental trauma and the efficacy of intentional replantation with retrograde root canal filling for a transverse root fracture in an immature incisor accompanied by subsequent periodontal-endodontic disease. In the treatment of traumatically fractured roots in immature incisors, continual examination is indispensable for the final diagnosis because roots in immature teeth are less calcified, resulting in less detailed radiological examinations...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28408994/endoscopic-management-of-patients-with-post-surgical-leaks-involving-the-gastrointestinal-tract-a-large-case-series
#18
Raffaele Manta, Angelo Caruso, Carlo Cellini, Mariano Sica, Angelo Zullo, Vincenzo Giorgio Mirante, Helga Bertani, Marzio Frazzoni, Massimiliano Mutignani, Giuseppe Galloro, Rita Conigliaro
BACKGROUND: Post-surgical anastomotic leaks often require a re-intervention, are associated with a definite morbidity and mortality, and with relevant costs. We described a large series of patients with different post-surgical leaks involving the gastrointestinal tract managed with endoscopy as initial approach. METHODS: This was a retrospective analysis of prospectively collected cases with anastomotic leaks managed with different endoscopic approaches (with surgical or radiological drainage when needed) in two endoscopic centres during 5 years...
December 2016: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28378258/erratum-to-post-relapse-survival-in-patients-with-the-early-and-late-distant-recurrence-in-estrogen-receptor-positive-her2-negative-breast-cancer
#19
Akiko Ogiya, Kieko Yamazaki, Rie Horii, Tadahiko Shien, Yoshiya Horimoto, Norikazu Masuda, Touko Inao, Mitsuchika Hosoda, Naoko Ishida, Tomofumi Osako, Masato Takahashi, Yumi Endo, Yuichiro Miyoshi, Hiroyuki Yasojima, Nobumoto Tomioka, Hiroko Yamashita
No abstract text is available yet for this article.
April 4, 2017: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/28247824/long-term-follow-up-of-endo-vascular-recanalization-of-chronic-inferior-vena-cava-occlusion-secondary-to-inferior-vena-cava-filters
#20
Sasan Partovi, Sanjeeva P Kalva, T Gregory Walker, Sabir M Taj, Suvranu Ganguli
BACKGROUND: The long term efficacy of endovascular recanalization for chronic iliocaval occlusion secondary to inferior vena cava (IVC) filters is unknown. The purpose of this study was to evaluate the effectiveness of endovascular recanalization and stent placement across the filter in patients with filter-associated chronic iliocaval occlusion. PATIENTS AND METHODS: Seven patients (mean age 56 ± 15 yrs; seven males) with symptomatic chronic iliocaval occlusion and occluded IVC filter were included...
March 2017: VASA. Zeitschrift Für Gefässkrankheiten
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