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Bao-Tao Liu, Xiao-Yan Zhang, Shu-Wei Wan, Jun-Jie Hao, Rui-De Jiang, Feng-Jing Song
Vegetables harboring bacteria resistant to antibiotics are a growing food safety issue. However, data concerning carbapenem-resistant Enterobacteriaceae (CRE) in ready-to-eat fresh vegetables is still rare. In this study, 411 vegetable samples from 36 supermarkets or farmer's markets in 18 cities in China, were analyzed for CRE. Carbapenemase-encoding genes and other resistance genes were analyzed among the CRE isolates. Plasmids carrying carbapenemase genes were studied by conjugation, replicon typing, S1-PFGE southern blot, restriction fragment length polymorphism (RFLP), and sequencing...
2018: Frontiers in Microbiology
T Gelbíčová, M Zobaníková, Z Tomáštíková, I Van Walle, W Ruppitsch, R Karpíšková
Since 2012-2016 an increased number of listeriosis cases, especially from one region of the Czech Republic, were observed. Most of them were caused by strains of serotype 1/2a, clonal complex 8, indistinguishable by pulsed-field gel electrophoresis. Twenty-six human cases were reported, including two neonatal cases in twins. Three cases were fatal. The typing of Listeria monocytogenes isolates from food enabled to confirm a turkey meat delicatessen as the vehicle of infection for this local outbreak in the Moravian-Silesian Region...
June 18, 2018: Epidemiology and Infection
Hoda Ilkhani, Siamak Farhad
The aim of this study was to fabricate a novel electrochemical-based DNA-sensing device for Ebola virus DNA diagnostic by an enzyme-amplified detection, which improves the sensitivity and selectivity of the sensor. A thiolated DNA capture probe sequence was immobilized on the screen printed electrode surface and hybridized with biotinylated target strand DNA for the fabrication of Ebola DNA-sensing devices. Prior to the electrochemical detection of the enzymatic product by differential pulse voltammetry (DPV) method, the biotinylated hybrid was labelled with a streptavidin-alkaline phosphate conjugate on the surface of the working electrode...
June 13, 2018: Analytical Biochemistry
Peyman Sakhaii, Bojan Bohorc, Wolfgang Bermel
A new experiment for recording double quantum spectra is introduced. The 2D DQ NMR experiment yields phase sensitive spectra with double quantum frequencies in F1 . The appearance of remote peaks is vastly suppressed by using a small flip angle double quantum excitation and reconversion. Pulse sequences and phase sensitive processing are discussed. The complexity of the SQ antiphase magnetization given in larger proton spin networks could be reduced by using the option of band selective decoupling during the preparation period...
May 24, 2018: Journal of Magnetic Resonance
Massimiliano Orsini, Alessandra Cornacchia, Claudio Patavino, Marina Torresi, Patrizia Centorame, Vicdalia Aniela Acciari, Anna Ruolo, Maurilia Marcacci, Massimo Ancora, Marco Di Domenico, Iolanda Mangone, Giuliana Blasi, Anna Duranti, Cesare Cammà, Francesco Pomilio, Giacomo Migliorati
We report the whole-genome sequences of two Listeria monocytogenes strains responsible for a severe invasive listeriosis outbreak in central Italy that occurred in 2015 and 2016. These two strains differ by a single band in their pulsed-field gel electrophoresis (PFGE) profiles.
June 14, 2018: Genome Announcements
Catherine Herlta, Rosa Starka, Haukur L Sigmarsson, Johannes Kauffold
OBJECTIVE: To test for the feasibility of transabdominal Doppler sonography (color, power, pulse wave) to define uterine perfusion characteristics throughout the estrous cycle in gilts. MATERIALS AND METHODS: A total of 15 gilts were synchronized for estrus and scanned in their following spontaneous cycle while being restrained in a purpose-designed mobile crate. To define uterine perfusion characteristics, vessels in between and within uterine cross-sections were imaged and recorded as video sequences to be analyzed by PixelFlux® software for perfused area (Amix), blood flow velocity (vmix) and intensity (Imix) as well as resistance (RIvmix) and pulsatility index (PIvmix)...
June 2018: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
Colline Sanchez, Christine Berthier, Bruno Allard, Jimmy Perrot, Clément Bouvard, Hidekazu Tsutsui, Yasushi Okamura, Vincent Jacquemond
Ion channel activity in the plasma membrane of living cells generates voltage changes that are critical for numerous biological functions. The membrane of the endoplasmic/sarcoplasmic reticulum (ER/SR) is also endowed with ion channels, but whether changes in its voltage occur during cellular activity has remained ambiguous. This issue is critical for cell functions that depend on a Ca2+ flux across the reticulum membrane. This is the case for contraction of striated muscle, which is triggered by opening of ryanodine receptor Ca2+ release channels in the SR membrane in response to depolarization of the transverse invaginations of the plasma membrane (the t-tubules)...
June 13, 2018: Journal of General Physiology
Christine Martineau, Xuejing Li, Cindy Lalancette, Thérèse Perreault, Eric Fournier, Julien Tremblay, Milagros Gonzales, Étienne Yergeau, Caroline Quach
Serratia marcescens is an environmental bacterium commonly associated with outbreaks in neonatal intensive care units (NICU). Investigation of S. marcescens outbreaks requires efficient recovery and typing of clinical and environmental isolates. In this study, we described how the use of next-generation sequencing applications, such as bacterial whole-genome sequencing (WGS) and bacterial community profiling, could improve S. marcescens outbreak investigation. Phylogenomic links and potential antibiotic resistance genes and plasmids in S...
June 13, 2018: Journal of Clinical Microbiology
Gourvendu Saxena, Suparna Mitra, Ezequiel M Marzinelli, Chao Xie, Toh Jun Wei, Peter D Steinberg, Rohan B H Williams, Staffan Kjelleberg, Federico M Lauro, Sanjay Swarup
Growing demands for potable water have led to extensive reliance on waterways in tropical megacities. Attempts to manage these waterways in an environmentally sustainable way generally lack an understanding of microbial processes and how they are influenced by urban factors, such as land use and rain. Here, we describe the composition and functional potential of benthic microbial communities from an urban waterway network and analyze the effects of land use and rain perturbations on these communities. With a sequence depth of 3 billion reads from 48 samples, these metagenomes represent nearly full coverage of microbial communities...
May 2018: MSystems
Hua Li, Pengfei Wang, Ruiting Lan, Lijuan Luo, Xiaolong Cao, Yi Wang, Yan Wang, Hui Li, Lu Zhang, Shunshi Ji, Changyun Ye
Listeria monocytogenes can contaminate various foods via food processing environments and contamination of raw materials. There is a limited understanding of L. monocytogenes transmission in retail market and the role of insects in L. monocytogenes transmission in the retail environments. To better understand the risk factors of raw pork contamination, the prevalence of L. monocytogenes was examined in raw pork, retail environments and insects in a retail market over a 6-month period from March to August in 2016 in Beijing, China...
2018: Frontiers in Microbiology
Laleh Golestanirad, Amir Ali Rahsepar, John E Kirsch, Kenichiro Suwa, Jeremy C Collins, Leonardo M Angelone, Boris Keil, Rod S Passman, Giorgio Bonmassar, Peter Serano, Peter Krenz, Jim DeLap, James C Carr, Lawrence L Wald
PURPOSE: To evaluate the local specific absorption rate (SAR) and heating around retained cardiac leads during MRI at 64 MHz (1.5T) and 127 MHz (3T) as a function of RF coil type and imaging landmark. METHODS: Numerical models of retained cardiac leads were built from CT and X-ray images of 6 patients with retained cardiac leads. Electromagnetic simulations and bio-heat modeling were performed with MRI RF body and head coils tuned to 64 MHz and 127 MHz and positioned at 9 different imaging landmarks covering an area from the head to the lower limbs...
June 12, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
Jian-Woon Chen, Yin Yin Lau, Thiba Krishnan, Kok-Gan Chan, Chien-Yi Chang
Pseudomonas aeruginosa is a rod-shaped Gram-negative bacterium which is notably known as a pathogen in humans, animals, and plants. Infections caused by P. aeruginosa especially in hospitalized patients are often life-threatening and rapidly increasing worldwide throughout the years. Recently, multidrug-resistant P. aeruginosa has taken a toll on humans' health due to the inefficiency of antimicrobial agents. Therefore, the rapid and advanced diagnostic techniques to accurately detect this bacterium particularly in clinical samples are indeed necessary to ensure timely and effective treatments and to prevent outbreaks...
2018: Frontiers in Microbiology
Ahmadreza Keihani, Zahra Shirzhiyan, Morteza Farahi, Elham Shamsi, Amin Mahnam, Bahador Makkiabadi, Mohsen R Haidari, Amir H Jafari
Background: Recent EEG-SSVEP signal based BCI studies have used high frequency square pulse visual stimuli to reduce subjective fatigue. However, the effect of total harmonic distortion (THD) has not been considered. Compared to CRT and LCD monitors, LED screen displays high-frequency wave with better refresh rate. In this study, we present high frequency sine wave simple and rhythmic patterns with low THD rate by LED to analyze SSVEP responses and evaluate subjective fatigue in normal subjects. Materials and Methods: We used patterns of 3-sequence high-frequency sine waves (25, 30, and 35 Hz) to design our visual stimuli...
2018: Frontiers in Human Neuroscience
Martin Worm, Tim Landgraf, Julia Prume, Hai Nguyen, Frank Kirschbaum, Gerhard von der Emde
Mormyrid weakly electric fish produce electric organ discharges (EODs) for active electrolocation and electrocommunication. These pulses are emitted with variable interdischarge intervals (IDIs) resulting in temporal discharge patterns and interactive signaling episodes with nearby conspecifics. However, unequivocal assignment of interactive signaling to a specific behavioral context has proven to be challenging. Using an ethorobotical approach, we confronted single individuals of weakly electric Mormyrus rume proboscirostris with a mobile fish robot capable of interacting both physically, on arbitrary trajectories, as well as electrically, by generating echo responses through playback of species-specific EODs, thus synchronizing signals with the fish...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Xingcan Qian, Qing Qu, Lei Li, Xin Ran, Limei Zuo, Rui Huang, Qiang Wang
Foodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and CeO₂ nanorod has been utilized as sensing material to achieve sensitive and selective detection of C. perfringens DNA sequence due to its strong adsorption ability towards DNA compared to nanoparticle. The DNA probe was tightly immobilized on CeO₂/chitosan modified electrode surface via metal coordination, and the DNA surface density was 2...
June 8, 2018: Sensors
Xiaogang Niu, Jienv Ding, Wenbo Zhang, Qianwen Li, Yunfei Hu, Changwen Jin
Proteins are intrinsically dynamic molecules and undergo exchanges among multiple conformations to perform biological functions. The CPMG relaxation dispersion and CEST experiments are two important solution NMR techniques for characterizing the conformational exchange processes on the millisecond timescale. Traditional pseudo 3D 15 N CEST and CPMG experiments have certain limitations in their applications. For example, both experiments have low sensitivity for broadened resonances, and the process of optimizing sample conditions and experimental parameters are often time consuming...
June 1, 2018: Journal of Magnetic Resonance
Shinya Nawata, Atsuto Maki, Takashi Hikihara
A power packet is a unit of electric power composed of a power pulse and an information tag. In Shannon's information theory, messages are represented by symbol sequences in a digitized manner. Referring to this formulation, we define symbols in power packetization as a minimum unit of power transferred by a tagged pulse. Here, power is digitized and quantized. In this paper, we consider packetized power in networks for a finite duration, giving symbols and their energies to the networks. A network structure is defined using a graph whose nodes represent routers, sources and destinations...
May 2018: Proceedings. Mathematical, Physical, and Engineering Sciences
Dong He, Yanxin An, Yanhua Li, Jing Wang, Gaoyi Wu, Lei Chen, Guoxiong Zhu
PURPOSE: To explore the potential molecular mechanism of low-intensity pulsed ultrasound (LIPUS) in the treatment of temporomandibular joint osteoarthritis (TMJ-OA), and identify the target genes for therapy of TMJ-OA. METHODS: Rat TMJ-OA was induced by unilateral occlusal trauma (UOT). At 8 weeks, the experimental group rats were treated by LIPUS for 4 weeks (5 days every week). The cartilage was examined by histological techniques. Gene expression profile in control, placebo and LIPUS-treated group were measured by RNA sequencing (RNA-Seq)...
June 6, 2018: Gene
Maren Mylius, Johannes Dreesman, Matthias Pulz, Gerhard Pallasch, Konrad Beyrer, Katja Claußen, Franz Allerberger, Angelika Fruth, Christina Lang, Rita Prager, Antje Flieger, Sabine Schlager, Daniela Kalhöfer, Elke Mertens
Following a school ski-trip to Austria from 10 to 18/02/2017, nine of 25 participants of the group from Lower Saxony (Germany) developed gastroenteritis. The students and teachers (17-41 years) shared meals in a hotel. Active case finding revealed further cases among German school groups from North Rhine-Westphalia and Schleswig-Holstein, staying at the same hotel in February 2017. We conducted two retrospective cohort studies using self-administered questionnaires on clinical symptoms and food consumption...
May 29, 2018: International Journal of Medical Microbiology: IJMM
Sameer Ahmed Alsharapy, Betitera Yanat, Lorena Lopez-Cerero, Samar Saeed Nasher, Paula Díaz-De-Alba, Álvaro Pascual, José-Manuel Rodríguez-Martínez, Abdelaziz Touati
OBJECTIVE: The aim of this study was to characterize the O25b/ST131 clone in ciprofloxacin-resistant Escherichia coli isolates from Yemen. MATERIALS AND METHODS: A total of 41 ciprofloxacin-resistant E. coli strains were collected from clinical samples of inpatients and outpatients from Sana'a (Yemen) from January to December 2013. Antimicrobial susceptibility testing, polymerase chain reaction amplification, and sequencing were used for detection of plasmid-mediated quinolone resistance determinants, extended-spectrum beta-lactamases genes and mutations in the quinolone resistance-determining regions of the target genes gyrA and parC...
June 8, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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