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https://www.readbyqxmd.com/read/28346073/detection-of-clinically-significant-prostate-cancer-short-dual-pulse-sequence-versus-standard-multiparametric-mr-imaging-a-multireader-study
#1
Borna K Barth, Pieter J L De Visschere, Alexander Cornelius, Carlos Nicolau, Hebert Alberto Vargas, Daniel Eberli, Olivio F Donati
Purpose To compare the diagnostic performance of a short dual-pulse sequence magnetic resonance (MR) imaging protocol versus a standard six-pulse sequence multiparametric MR imaging protocol for detection of clinically significant prostate cancer. Materials and Methods This HIPAA-compliant study was approved by the regional ethics committee. Between July 2013 and March 2015, 63 patients from a prospectively accrued study population who underwent MR imaging of the prostate including transverse T1-weighted; transverse, coronal, and sagittal T2-weighted; diffusion-weighted; and dynamic contrast material-enhanced MR imaging with a 3-T imager at a single institution were included in this retrospective study...
March 27, 2017: Radiology
https://www.readbyqxmd.com/read/28344780/abnormal-glycosylation-in-joubert-syndrome-type-10
#2
Megan S Kane, Mariska Davids, Michelle R Bond, Christopher J Adams, Megan E Grout, Ian G Phelps, Diana R O'Day, Jennifer C Dempsey, Xeuli Li, Gretchen Golas, Gilbert Vezina, Meral Gunay-Aygun, John A Hanover, Dan Doherty, Miao He, May Christine V Malicdan, William A Gahl, Cornelius F Boerkoel
BACKGROUND: The discovery of disease pathogenesis requires systematic agnostic screening of multiple homeostatic processes that may become deregulated. We illustrate this principle in the evaluation and diagnosis of a 5-year-old boy with Joubert syndrome type 10 (JBTS10). He carried the OFD1 mutation p.Gln886Lysfs*2 (NM_003611.2: c.2656del) and manifested features of Joubert syndrome. METHODS: We integrated exome sequencing, MALDI-TOF mass spectrometry analyses of plasma and cultured dermal fibroblasts glycomes, and full clinical evaluation of the proband...
2017: Cilia
https://www.readbyqxmd.com/read/28343367/magnetic-resonance-imaging-features-of-leukoaraiosis-in-elderly-dogs
#3
Elena Scarpante, Giunio Bruto Cherubini, Alberta de Stefani, Olivier Taeymans
Leukoaraiosis is a descriptive term used to designate bilateral, symmetrical, white matter lesions identified in brains of elderly human patients. These lesions are isointense to normal in magnetic resonance imaging (MRI) T1-weighted pulse sequences, non-contrast enhancing, and hyperintense in T2-weighted and FLAIR pulse sequences. Pathophysiologic mechanisms for leukoaraiosis remain incompletely understood; however, an ischemic origin is currently being favored. Age-related changes, such as brain atrophy, ventricular enlargement, and well-demarcated sulci, have also been previously described in dogs over 9 years of age...
March 26, 2017: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/28342606/increased-anaplerosis-of-the-tricarboxylic-acid-cycle-decreased-meal-size-and-energy-intake-of-cows-in-the-postpartum-period
#4
Laura B Gualdrón-Duarte, Michael S Allen
The objective of this study was to determine the effects of anaplerosis of the tricarboxylic acid cycle on feeding behavior and energy intake of cows in the postpartum period. We infused propionic acid (PA) and glycerol (GL) continuously into the abomasum and hypothesized that PA will decrease meal size and energy intake compared with GL because PA enters the tricarboxylic acid cycle, likely stimulating oxidation of acetyl CoA and satiety compared with GL. Three experiments (Exp.) were conducted using 20 Holstein cows between 3 and 22 d postpartum (8 cows in Exp...
March 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28340704/sub-attomolar-electrochemical-measurement-of-dna-hybridization-based-on-the-detection-of-high-coverage-biobarcode-latex-labels-at-pna-modified-screen-printed-electrodes
#5
Tarina Widaningrum, Endrika Widyastuti, Feby Wijaya Pratiwi, Ai Imas Faidoh Fatimah, Patsamon Rijiravanich, Mithran Somasundrum, Werasak Surareungchai
We have constructed biobarcode labels based on 468nm diameter latex spheres. Modification with polyallylamine and then glutaraldehyde was used to attach a high DNA loading, consisting of aminated probe DNA (approx. 1.01×10(2) molecules per sphere) and biobarcode DNA (approx. 1.66×10(4) molecules per sphere). Detection of the biobarcodes was performed by application of a Ag enhancer solution, causing association of the Ag(+) ions with the phosphate groups of the DNA. The deposited Ag was detected by differential pulse voltammetry...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28338012/prevalence-of-st1193-clone-and-inci1-st16-plasmid-in-e-coli-isolates-carrying-blactx-m-55-gene-from-urinary-tract-infections-patients-in-china
#6
Liang Xia, Yang Liu, Shu Xia, Timothy Kudinha, Shu-Nian Xiao, Nan-Shan Zhong, Guo-Sheng Ren, Chao Zhuo
To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 blaCTX-M-55-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analysis were performed to characterize plasmids harboring blaCTX-M-55 and their genetic environment. 111 isolates were clustered into 86 individual pulsotypes and three clusters by PFGE...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337187/defining-and-evaluating-a-core-genome-multilocus-sequence-typing-scheme-for-whole-genome-sequence-based-typing-of-klebsiella-pneumoniae
#7
Haijian Zhou, Wenbing Liu, Tian Qin, Chen Liu, Hongyu Ren
At present, the most used methods for Klebsiella pneumoniae subtyping are multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). However, the discriminatory power of MLST could not meet the need for distinguishing outbreak and non-outbreak isolates and the PFGE is time-consuming and labor-intensive. A core genome multilocus sequence typing (cgMLST) scheme for whole-genome sequence-based typing of K. pneumoniae was developed for solving the disadvantages of these traditional molecular subtyping methods...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28330895/speciation-and-strain-typing-of-staphylococcus-agnetis-and-staphylococcus-hyicus-isolated-from-bovine-milk-using-a-novel-multiplex-pcr-and-pulsed-field-gel-electrophoresis
#8
P R F Adkins, J R Middleton, M J Calcutt, G C Stewart, L K Fox
Staphylococcus hyicus and Staphylococcus agnetis are two coagulase variable staphylococcal species that can be isolated from bovine milk and are difficult to differentiate. The objectives of this study were to characterize isolates of bovine milk origin from a collection that had previously been characterized as coagulase positive S. hyicus based on phenotypic speciation methods and to develop a PCR-based method for differentiating S. hyicus, S. agnetis, and S. aureus. Isolates (n = 62) were selected from a previous study in which milk samples were collected from cows on 15 dairy herds...
March 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28330888/defining-a-core-genome-multilocus-sequence-typing-scheme-for-the-global-epidemiology-of-vibrio-parahaemolyticus
#9
Narjol Gonzalez-Escalona, Keith A Jolley, Elizabeth Reed, Jaime Martinez-Urtaza
Vibrio parahaemolyticus is an important human foodborne pathogen whose transmission is associated with the consumption of contaminated seafood with a growing number of infections reported over recent years worldwide. A multilocus sequence typing (MLST) database for V. parahaemolyticus was created in 2008 and a large number of clones have been identified causing severe outbreaks worldwide (ST3), recurrent outbreaks in certain regions (e.g., ST36) or spreading to other regions where they are non-endemic (e.g...
March 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28330349/coherent-manipulation-of-non-thermal-spin-order-in-optical-nuclear-polarization-experiments
#10
Gerd Buntkowsky, Konstantin L Ivanov, Herbert Zimmermann, Hans-Martin Vieth
Time resolved measurements of Optical Nuclear Polarization (ONP) have been performed on hyperpolarized triplet states in molecular crystals created by light excitation. Transfer of the initial electron polarization to nuclear spins has been studied in the presence of radiofrequency excitation; the experiments have been performed with different pulse sequences using different doped molecular systems. The experimental results clearly demonstrate the dominant role of coherent mechanisms of spin order transfer, which manifest themselves in well pronounced oscillations...
March 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28328967/phenotypic-and-molecular-characterization-of-antimicrobial-resistance-in-enterobacter-spp-isolates-from-companion-animals-in-japan
#11
Kazuki Harada, Takae Shimizu, Yujiro Mukai, Ken Kuwajima, Tomomi Sato, Akari Kajino, Masaru Usui, Yutaka Tamura, Yui Kimura, Tadashi Miyamoto, Yuzo Tsuyuki, Asami Ohki, Yasushi Kataoka
The emergence of antimicrobial resistance among Enterobacter spp., including resistance to extended-spectrum cephalosporins (ESC), is of great concern in both human and veterinary medicine. In this study, we investigated the prevalence of antimicrobial resistance among 60 isolates of Enterobacter spp., including E. cloacae (n = 44), E. aerogenes (n = 10), and E. asburiae (n = 6), from clinical specimens of dogs and cats from 15 prefectures in Japan. Furthermore, we characterized the resistance mechanisms harbored by these isolates, including extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR); and assessed the genetic relatedness of ESC-resistant Enterobacter spp...
2017: PloS One
https://www.readbyqxmd.com/read/28328013/validation-of-surface-to-volume-ratio-measurements-derived-from-oscillating-gradient-spin-echo-on-a-clinical-scanner-using-anisotropic-fiber-phantoms
#12
Gregory Lemberskiy, Steven H Baete, Martijn A Cloos, Dmitry S Novikov, Els Fieremans
A diffusion measurement in the short-time surface-to-volume ratio (S/V) limit (Mitra et al., Phys Rev Lett. 1992;68:3555) can disentangle the free diffusion coefficient from geometric restrictions to diffusion. Biophysical parameters, such as the S/V of tissue membranes, can be used to estimate microscopic length scales non-invasively. However, due to gradient strength limitations on clinical MRI scanners, pulsed gradient spin echo (PGSE) measurements are impractical for probing the S/V limit. To achieve this limit on clinical systems, an oscillating gradient spin echo (OGSE) sequence was developed...
March 22, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28324122/mr-imaging-differentiation-of-fe-2-and-fe-3-based-on-relaxation-and-magnetic-susceptibility-properties
#13
Olaf Dietrich, Johannes Levin, Seyed-Ahmad Ahmadi, Annika Plate, Maximilian F Reiser, Kai Bötzel, Armin Giese, Birgit Ertl-Wagner
PURPOSE: The aim of this study is to evaluate the MR imaging behavior of ferrous (Fe(2+)) and ferric (Fe(3+)) iron ions in order to develop a noninvasive technique to quantitatively differentiate between both forms of iron. METHODS: MRI was performed at 3 T in a phantom consisting of 21 samples with different concentrations of ferrous and ferric chloride solutions (between 0 and 10 mmol/L). A multi-echo spoiled gradient-echo pulse sequence with eight echoes was used for both T 2* and quantitative susceptibility measurements...
March 21, 2017: Neuroradiology
https://www.readbyqxmd.com/read/28321918/broadband-rf-amplitude-dependent-flip-angle-pulses-with-linear-phase-slope
#14
Martin R M Koos, Hannes Feyrer, Burkhard Luy
Pulse sequences in NMR spectroscopy sometimes require the application of pulses with effective flip angles different from 90˚ and 180˚ Previously (Magn. Reson. Chem.), offset-compensated broadband excitation pulses with rf-amplitude-dependent effective flip angles (RADFA) were introduced that are applicable in such cases. However, especially rf-amplitude-restricted RADFA pulses turned out to perform not as good as desired in terms of achievable bandwidths. Here, a class of rf-amplitude-restricted RADFA pulses with linear phase slope is introduced that allows excitation over much larger bandwidths with better performance...
March 20, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28321915/acceleration-of-asl-based-time-resolved-mr-angiography-by-acquisition-of-control-and-labeled-images-in-the-same-shot-actress
#15
Yuriko Suzuki, Noriyuki Fujima, Tetsuo Ogino, James Alastair Meakin, Akira Suwa, Hiroyuki Sugimori, Marc Van Cauteren, Matthias J P van Osch
PURPOSE: Noncontrast 4D-MR-angiography (MRA) using arterial spin labeling (ASL) is beneficial because high spatial and temporal resolution can be achieved. However, ASL requires acquisition of labeled and control images for each phase. The purpose of this study is to present a new accelerated 4D-MRA approach that requires only a single control acquisition, achieving similar image quality in approximately half the scan time. METHODS: In a multi-phase Look-Locker sequence, the first phase was used as the control image and the labeling pulse was applied before the second phase...
March 20, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28321908/reduced-field-of-view-single-shot-spiral-perfusion-imaging
#16
Yang Yang, Li Zhao, Xiao Chen, Peter W Shaw, Jorge A Gonzalez, Frederick H Epstein, Craig H Meyer, Christopher M Kramer, Michael Salerno
PURPOSE: To develop a single-shot spiral perfusion pulse sequence with outer-volume suppression (OVS) to achieve whole-heart coverage with a short temporal footprint of 10 ms per slice location. METHODS: A highly accelerated single-shot variable density spiral pulse sequence with an integrated OVS module for reduced field of view (rFOV) perfusion imaging with 2 mm spatial resolution was developed and evaluated in simulations, phantom experiments and in clinical patients with (n = 8) or without (n = 8) OVS...
March 20, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28321904/rotating-single-shot-acquisition-rosa-with-composite-reconstruction-for-fast-high-resolution-diffusion-imaging
#17
Qiuting Wen, Chandana Kodiweera, Brian M Dale, Giri Shivraman, Yu-Chien Wu
PURPOSE: To accelerate high-resolution diffusion imaging, rotating single-shot acquisition (RoSA) with composite reconstruction is proposed. Acceleration was achieved by acquiring only one rotating single-shot blade per diffusion direction, and high-resolution diffusion-weighted (DW) images were reconstructed by using similarities of neighboring DW images. A parallel imaging technique was implemented in RoSA to further improve the image quality and acquisition speed. RoSA performance was evaluated by simulation and human experiments...
March 20, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28320598/molecular-characterization-of-ureaplasmas-isolated-from-reproductive-tract-of-goats-and-sheep-from-brazil
#18
Rosângela C Oliveira, Maurício Yamaguti, Lucas M Marques, Melissa Buzinhani, Huber Rizzo, Enoch Brandão de Souza Meira-Junior, Ana Márcia S Guimarães, Lilian Gregory, Jorge Timenetsky
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility...
February 20, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28319904/sensitivity-enhancement-of-graphene-zinc-oxide-nanocomposite-based-electrochemical-impedance-genosensor-for-single-stranded-rna-detection
#19
Sze Shin Low, Hwei-San Loh, Jian Sheng Boey, Poi Sim Khiew, Wee Siong Chiu, Michelle T T Tan
An efficient electrochemical impedance genosensing platform has been constructed based on graphene/zinc oxide nanocomposite produced via a facile and green approach. Highly pristine graphene was synthesised from graphite through liquid phase sonication and then mixed with zinc acetate hexahydrate for the synthesis of graphene/zinc oxide nanocomposite by solvothermal growth. The as-synthesised graphene/zinc oxide nanocomposite was characterised with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy and X-ray diffractometry (XRD) to evaluate its morphology, crystallinity, composition and purity...
February 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28319821/application-of-p4-polyphase-codes-pulse-compression-method-to-air-coupled-ultrasonic-testing-systems
#20
Honggang Li, Zhenggan Zhou
Air-coupled ultrasonic testing systems are usually restricted by low signal-to-noise ratios (SNR). The use of pulse compression techniques based on P4 Polyphase codes can improve the ultrasound SNR. This type of codes can generate higher Peak Side Lobe (PSL) ratio and lower noise of compressed signal. This paper proposes the use of P4 Polyphase sequences to code ultrasound with a NDT system based on air-coupled piezoelectric transducer. Furthermore, the principle of selecting parameters of P4 Polyphase sequence for obtaining optimal pulse compression effect is also studied...
March 3, 2017: Ultrasonics
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