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https://www.readbyqxmd.com/read/29684765/application-of-maldi-tof-ms-for-the-subtyping-of-arcobacter-butzleri-strains-and-comparison-with-their-mlst-and-pfge-types
#1
Federica Giacometti, Silvia Piva, Katleen Vranckx, Katrien De Bruyne, Ilenia Drigo, Alex Lucchi, Gerardo Manfreda, Andrea Serraino
For the first time, this study evaluated the use of MALDI-TOF as a typing tool for Arcobacter butzleri. A total of 104 A. butzleri strains isolated from different sources in an artisanal dairy plant in Italy were identified and typed using MALDI-TOF and compared with their multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) profiles found in previous studies. MALDI-TOF correctly identified all the isolates to species level. No clearly delineated clusters appeared on dendrograms based on either the complete spectra or the significant peaks, but nine clusters were defined using the cophenetic correlation...
April 16, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29681278/electrochemical-detection-of-sequence-specific-dna-based-on-formation-of-g-quadruplex-hemin-through-continuous-hybridization-chain-reaction
#2
Xiaofan Sun, Haohan Chen, Shuling Wang, Yiping Zhang, Yaping Tian, Nandi Zhou
A high-sensitive detection of sequence-specific DNA was established based on the formation of G-quadruplex-hemin complex through continuous hybridization chain reaction (HCR). Taking HIV DNA sequence as an example, a capture probe complementary to part of HIV DNA was firstly self-assembled onto the surface of Au electrode. Then a specially designed assistant probe with both terminals complementary to the target DNA and a G-quadruplex-forming sequence in the center was introduced into the detection solution...
August 27, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29679477/generation-of-8-oxo-7-8-dihydroguanine-in-g-quadruplexes-models-of-human-telomer-sequences-by-one-electron-oxidation
#3
Tomasz J Merta, Nicholas E Geacintov, Vladimir Shafirovich
The mechanistic aspects of one-electron oxidation of G-quadruplexes in the basket (Na+ ions) and hybrid (K+ ions) conformations were investigated by transient absorption laser kinetic spectroscopy and HPLC detection of the 8-oxo-7,8-dihydroguanine (8-oxoG) oxidation product. The photo-induced one-electron abstraction from G-quadruplexes was initiated by sulfate radical anions (SO4 •- ) derived from the photolysis of persulfate ions by 308 nm excimer laser pulses. In neutral aqueous solutions (pH 7.0), the transient absorbance of neutral guanine radicals, G(-H)• is observed following the complete decay of SO4 •- radicals (~10 μs after the actinic laser flash)...
April 21, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29678540/quantitative-diffusion-mri-using-reduced-field-of-view-and-multi-shot-acquisition-techniques-validation-in-phantoms-and-prostate-imaging
#4
Yuxin Zhang, James Holmes, Iñaki Rabanillo, Arnaud Guidon, Shane Wells, Diego Hernando
PURPOSE: To evaluate the reproducibility of quantitative diffusion measurements obtained with reduced Field of View (rFOV) and Multi-shot EPI (msEPI) acquisitions, using single-shot EPI (ssEPI) as a reference. METHODS: Diffusion phantom experiments, and prostate diffusion-weighted imaging in healthy volunteers and patients with known or suspected prostate cancer were performed across the three different sequences. Quantitative diffusion measurements of apparent diffusion coefficient, and diffusion kurtosis parameters (healthy volunteers), were obtained and compared across diffusion sequences (rFOV, msEPI, and ssEPI)...
April 17, 2018: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/29678299/molecular-epidemiology-of-klebsiella-pneumoniae-k1-and-k2-isolates-in-japan
#5
Sohei Harada, Yoshikazu Ishii, Tomoo Saga, Kotaro Aoki, Kazuhiro Tateda
Although severe infections caused by hypervirulent Klebsiella pneumoniae isolates, such as K1 isolates belonging to sequence type (ST) 23, have been a significant problem in Asian countries, epidemiology of these isolates in Japan remains unclear. We performed a nationwide molecular epidemiological study of K. pneumoniae K1 and K2 isolates in Japan. Of the 259K. pneumoniae isolates collected, 14 and 16 isolates were identified as capsular genotypes K1 and K2, respectively. All K1 isolates were ST23 or its closely related clones and showed high genetic similarity by pulsed-field gel electrophoresis (PFGE) and the DiversiLab system (DL)...
March 20, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29677467/vibrational-and-nonadiabatic-coherence-in-2d-electronic-spectroscopy-the-jahn-teller-effect-and-energy-transfer
#6
David M Jonas
Femtosecond two-dimensional (2D) Fourier transform spectroscopy generates and probes several types of coherence that characterize the couplings between vibrational and electronic motions. These couplings have been studied in molecules with Jahn-Teller conical intersections, pseudo-Jahn-Teller funnels, dimers, molecular aggregates, photosynthetic light harvesting complexes, and photosynthetic reaction centers. All have closely related Hamiltonians and at least two types of vibrations, including one that is decoupled from the electronic dynamics and one that is nonadiabatically coupled...
April 20, 2018: Annual Review of Physical Chemistry
https://www.readbyqxmd.com/read/29676727/characterization-of-sccmec-type-iv-methicillin-resistant-staphylococcus-aureus-clones-increased-in-japanese-hospitals
#7
Hidemasa Nakaminami, Shunsuke Takadama, Ayumu Ito, Mariko Hasegawa, Chika Jono, Miyuki Noguchi, Manami Shoshi, Takeaki Wajima, Takeshi Fujii, Hiroshi Maruyama, Haruo Sakamoto, Yutaka Ito, Satsuki Okamoto, Yukiyoshi Masaki, Kayoko Tsuchiya, Susumu Nishinarita, Norihisa Noguchi
Recently, the prevalence of staphylococcal cassette chromosome mec (SCCmec) type IV isolates, which are the major community-acquired methicillin-resistant Staphylococcus aureus (MRSA), have increased in Japanese hospitals. The aim of this study was to elucidate the detailed molecular epidemiological features of the SCCmec type IV clones in Japanese hospitals. When 2589 MRSA isolated from four hospitals in Tokyo, Japan between 2010 and 2014 were analysed, the proportion of SCCmec type IV overtook that of type II, which was the major type of hospital-acquired MRSA in 2014...
April 20, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29672479/protracted-outbreak-of-salmonella-newport-infections-linked-to-ground-beef-possible-role-of-dairy-cows-21-states-2016-2017
#8
Katherine E Heiman Marshall, Mackenzie Tewell, Selam Tecle, Molly Leeper, Jennifer Sinatra, Bonnie Kissler, Adrienne Fung, Kerri Brown, Darlene Wagner, Eija Trees, Kelley B Hise, Vishnu Chaturvedi, Linda K Schlater, Brenda R Morningstar-Shaw, Laura Whitlock, Kristin Holt, Karen Becker, Megin Nichols, Ian T Williams, Michael Jhung, Matthew E Wise, Laura Gieraltowski
In January 2017, CDC identified a cluster of Salmonella enterica serotype Newport infections with isolates sharing an indistinguishable pulsed-field gel electrophoresis (PFGE) pattern, JJPX01.0010 (pattern 10), through PulseNet, the national molecular subtyping network for foodborne disease surveillance. This report summarizes the investigation by CDC, state and local health and agriculture departments, and the U.S. Department of Agriculture's Food Safety and Inspection Service (USDA-FSIS) and discusses the possible role of dairy cows as a reservoir for strains of Salmonella that persistently cause human illness...
April 20, 2018: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29666765/characterization-of-antimicrobial-resistance-genes-in-haemophilus-parasuis-isolated-from-pigs-in-china
#9
Yongda Zhao, Lili Guo, Jie Li, Xianhui Huang, Binghu Fang
Background: Haemophilus parasuis is a common porcine respiratory pathogen that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for antimicrobial susceptibility against six fluoroquinolone antibiotics testing by the broth microdilution method, and the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis...
2018: PeerJ
https://www.readbyqxmd.com/read/29665444/ndm-1-and-oxa-23-producing-acinetobacter-baumannii-isolated-from-intensive-care-unit-patients-in-tunisia
#10
Elaa Maamar, Carla Andrea Alonso, Sana Ferjani, Ali Jendoubi, Zaineb Hamzaoui, Alia Jebri, Mabrouka Saidani, Salma Ghedira, Carmen Torres, Ilhem Boutiba-Ben Boubaker
Gastrointestinal colonization by carbapenem-resistant Acinetobacter baumannii (CRAB), is a critical step before nosocomial infection. Our objective was to evaluate CRAB intestinal carriage in patients admitted in the intensive care unit (ICU) of a Tunisian Hospital and to determine the antimicrobial resistance mechanisms involved. From December-2014 to February-2015, all 63 patients admitted at the ICU were screened for rectal CRAB-colonization upon admission and once weekly thereafter. ICU-patients who acquired a CRAB-nosocomial-infection were also included...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29663108/trosy-pulse-sequence-for-simultaneous-measurement-of-the-15-n-r-1-and-1-h-15-n-noe-in-deuterated-proteins
#11
Paul A O'Brien, Arthur G Palmer
A TROSY-based NMR experiment is described for simultaneous measurement of the 15 N longitudinal relaxation rate constant R1 and the {1 H}-15 N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges 1 H and 15 N magnetizations. The accuracy of the proposed technique is validated by comparison to independent measurements of both relaxation parameters for the protein ubiquitin. The simultaneous experiment is approximately 20-33% shorter than conventional sequential measurements...
April 16, 2018: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/29660905/differences-in-time-domain-and-spectral-indexes-of-skin-surface-laser-doppler-signals-between-controls-and-breast-cancer-subjects
#12
Hsin Hsiu, Chao-Tsung Chen, Shuo-Hui Hung, Guan-Zhang Chen, Yu-Ling Huang
BACKGROUND: There is an urgent need to improve the early diagnosis of breast cancer. OBJECTIVE: The present study applied spectral and beat-to-beat analyses to laser-Doppler (LDF) data sequences measured on the skin surface on the back of the right hands, with the aim of comparing the different peripheral microcirculatory-blood-flow (MBF) perfusion condition between breast-cancer and control subjects. METHODS: ECG and LDF signals were obtained simultaneously and noninvasively from 23 breast-cancer patients and 23 age-matched control subjects...
April 13, 2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29659477/alpha-toxin-contributes-to-biofilm-formation-among-staphylococcus-aureus-wound-isolates
#13
Michele J Anderson, Emily Schaaf, Laura M Breshears, Heidi W Wallis, James R Johnson, Christine Tkaczyk, Bret R Sellman, Jisun Sun, Marnie L Peterson
Biofilms complicate treatment of Staphylococcus aureus (SA) wound infections. Previously, we determined alpha-toxin (AT)-promoted SA biofilm formation on mucosal tissue. Therefore, we evaluated SA wound isolates for AT production and biofilm formation on epithelium and assessed the role of AT in biofilm formation. Thirty-eight wound isolates were molecularly typed by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (ST), and spa typing. We measured biofilm formation of these SA isolates in vitro and ex vivo and quantified ex vivo AT production...
April 16, 2018: Toxins
https://www.readbyqxmd.com/read/29656053/novel-variant-ndm-11-and-other-ndm-1-variants-in-multidrug-resistant-escherichia-coli-from-south-india
#14
Mohibur Rahman, Chiranjay Mukhopadhyay, Ravi Prakash Rai, Sanjay Singh, Shefali Gupta, Avinash Singh, Ashutosh Pathak, Kashi Nath Prasad
OBJECTIVE: New Delhi metallo-β-lactamase (NDM-1) and its variants mediated carbapenem resistance has raised a major public health concern worldwide. In the present study, we report Escherichia coli positive novel variant i.e. NDM-11 for the first time METHODS: blaNDM were investigated in E. coli by PCR and sequencing. The blaNDM variants were further characterized. The susceptibility patterns of novel blaNDM-11 towards different antibiotics were compared with blaNDM-1 by cloning expressing in E...
April 12, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29649342/a-homogeneous-electrochemical-sensor-for-hg-2-determination-in-environmental-water-based-on-the-t-hg-2-t-structure-and-exonuclease-iii-assisted-recycling-amplification
#15
Caili Fu, Hao Yu, Lingshan Su, Chang Liu, Yanling Song, Shaoyun Wang, Zhenyu Lin, Fang Chen
A simple, fast, sensitive, and homogeneous electrochemical sensor based on the T-Hg2+-T structure and exonuclease III-assisted recycling amplification has been constructed for mercury ion (Hg2+) detection. The cT and methylene blue-labeled DNA probes (MB-TDNA) were designed to contain poly T sequences, which were repulsed from the negatively charged indium tin oxide (ITO) electrode due to their abundant negative charges. Hg2+ could trigger the formation of double-stranded DNA (dsDNA) between two DNA probes owing to the stable T-Hg2+-T structure...
April 12, 2018: Analyst
https://www.readbyqxmd.com/read/29643483/alteration-of-the-magnetosphere-of-the-vela-pulsar-during-a-glitch
#16
Jim Palfreyman, John M Dickey, Aidan Hotan, Simon Ellingsen, Willem van Straten
As pulsars lose energy, primarily in the form of magnetic dipole radiation, their rotation slows down accordingly. For some pulsars, this spin-down is interrupted by occasional abrupt spin-up events known as glitches 1 . A glitch is hypothesized to be a catastrophic release of pinned vorticity 2 that provides an exchange of angular momentum between the superfluid outer core and the crust. This is manifested by a minute alteration in the rotation rate of the neutron star and its co-rotating magnetosphere, which is revealed by an abrupt change in the timing of observed radio pulses...
April 2018: Nature
https://www.readbyqxmd.com/read/29643197/whole-genome-sequencing-of-recent-listeria-monocytogenes-isolates-from-germany-reveals-population-structure-and-disease-clusters
#17
Sven Halbedel, Rita Prager, Stephan Fuchs, Eva Trost, Guido Werner, Antje Flieger
Listeria monocytogenes causes food-borne outbreaks with high mortality. For improvement of outbreak cluster detection, the German consiliary laboratory for listeriosis implemented whole genome sequencing (WGS) in 2015. 424 human L. monocytogenes isolates collected in 2007-2017 were subjected to WGS and core genome multi locus sequence typing (cgMLST). cgMLST grouped the isolates into 38 complexes, reflecting four known and 34 unknown disease clusters. Most of these complexes were confirmed by single nucleotide polymorphism (SNP) calling but some further differentiated...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29628918/identification-and-characterization-of-fluoroquinolone-non-susceptible-streptococcus-pyogenes-clones-harboring-tetracycline-and-macrolide-resistance-in-shanghai-china
#18
Yinfang Shen, Jiehao Cai, Mark R Davies, Chi Zhang, Kun Gao, Dan Qiao, Haoqin Jiang, Weilei Yao, Yuefang Li, Mei Zeng, Mingliang Chen
Streptococcus pyogenes , also known as group A Streptococcus (GAS), is one of the top 10 infectious causes of death worldwide. Macrolide and tetracycline resistant GAS has emerged as a major health concern in China coinciding with an ongoing scarlet fever epidemic. Furthermore, increasing rates of fluoroquinolone (FQ) non-susceptibility within GAS from geographical regions outside of China has also been reported. Fluoroquinolones are the third most commonly prescribed antibiotic in China and is an therapeutic alternative for multi-drug resistant GAS...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29626930/link-between-double-strand-dna-break-hotspots-and-transcription-regulation-forum-domains-50-250-kb-chromosome-regions-containing-coordinately-expressed-genes
#19
REVIEW
N A Tchurikov, Y V Kravatsky, O V Kretova
The data on forum domains formed by DNA double-strand break (DSB) hotspots are reviewed including forum domain identification by pulsed-field gel electrophoresis, whole genome mapping of these domains using deep sequencing strategies, analysis of gene expression in forum domains, and binding of nuclear proteins to their boundaries. Earlier unpublished data by the authors are presented. The "piano playing" hypothesis is suggested based on coordinated active transcription in some of the forum domains and coordinated silencing in the majority of them...
April 2018: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29621600/nosocomial-transmission-of-carbapenem-resistant-klebsiella-pneumoniae-in-an-italian-university-hospital-a-molecular-epidemiological-study
#20
Giovanni Sotgiu, Bianca Maria Are, Lucia Pesapane, Alessandra Palmieri, Narcisa Muresu, Andrea Cossu, Marco Dettori, Antonio Azara, Ida Iolanda Mura, Clementina Cocuzza, Stefano Aliberti, Andrea Piana
AIM: To describe the phenotypic and genotypic profiles of Klebsiella pneumonia carbapenemase-producing K. pneumonia (KPC-Kp) strains isolated from patients with invasive infections at an Italian university hospital in order to assess the epidemiological trend. METHODS: An observational prospective study was carried out at the University Hospital of Sassari, Italy, to detect KPC-Kp strains in patients with invasive bacteraemia. Isolates were identified phenotypically; carbapenemase production was assessed using phenotypic and genotypic methods...
April 2, 2018: Journal of Hospital Infection
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