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Nuclear magnetic resonance

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https://www.readbyqxmd.com/read/28729737/direct-binding-to-gabarap-family-members-is-essential-for-hiv-1-nef-plasma-membrane-localization
#1
Alexandra Boeske, Melanie Schwarten, Peixiang Ma, Markus Tusche, Jessica Mötter, Christina Möller, Philipp Neudecker, Silke Hoffmann, Dieter Willbold
HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis. Studies have shown that the association of Nef with the inner leaflet of the plasma membrane and with endocytic and perinuclear vesicles is essential for most activities of Nef. Using purified recombinant proteins in pull-down assays and by co-immunoprecipitation assays we demonstrate that Nef binds directly and specifically to all GABARAP family members, but not to LC3 family members. Based on nuclear magnetic resonance (NMR) experiments we showed that Nef binds to GABARAP via two surface exposed hydrophobic pockets...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28728982/nmr-studies-of-large-proteins
#2
REVIEW
Yajun Jiang, Charalampos G Kalodimos
Recent breakthroughs in isotope-labeling and pulse sequence techniques have enabled the Nuclear Magnetic Resonance (NMR) characterization of large protein systems with molecular weights of hundreds of kDa. NMR studies of a great variety of large proteins have provided unique insights into the binding, dynamic, and allosteric mechanisms. Here we present a brief summary of these developments by highlighting few cases that exemplify the uniqueness of NMR in providing atomic-resolution information into key dynamic processes and structures of protein complexes with high degree of flexibility...
July 17, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28727439/solid-state-nmr-study-of-the-cicada-wing
#3
John David Gullion, Terry Gullion
Wings of flying insects are part of the cuticle which forms the exoskeleton. The primary molecular components of cuticle are protein, chitin, and lipid. How these components interact with one another to form the exoskeleton is not completely understood. The difficulty in characterizing the cuticle arises because it is insoluble and noncrystalline. These properties severely limit the experimental tools that can be used for molecular characterization. Solid-state nuclear magnetic resonance experiments have been used in the past to characterize the exoskeleton of beetles and have found that chitin and protein make comparable contributions to the molecular matrix...
July 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28726106/arginine-derivatives-of-dicarboxylic-acids-from-the-parotid-gland-secretions-of-common-toad-bufo-bufo-new-agonists-of-ionotropic-%C3%AE-aminobutyric-acid-receptors
#4
D S Lebedev, I A Ivanov, E V Kryukova, V G Starkov, V I Tsetlin, Yu N Utkin
Compounds activating γ-aminobutyric acid type A receptor were isolated from the toad Bufo bufo venom as a result of chromatographic separation. Analysis of the structure of these compounds by mass spectrometry and nuclear magnetic resonance showed that they are arginine derivatives of dicarboxylic acids and represent suberylarginine, pimeloylarginine, and adipoylarginine.
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28724919/generally-applicable-transformation-protocols-for-fluorescent-nanodiamond-internalization-into-cells
#5
Simon R Hemelaar, Kiran J van der Laan, Sophie R Hinterding, Manon V Koot, Else Ellermann, Felipe P Perona-Martinez, David Roig, Severin Hommelet, Daniele Novarina, Hiroki Takahashi, Michael Chang, Romana Schirhagl
Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724580/il-7-restores-t-lymphocyte-immunometabolic-failure-in-septic-shock-patients-through-mtor-activation
#6
Fabienne Venet, Julie Demaret, Benjamin J Blaise, Christelle Rouget, Thibaut Girardot, Estellie Idealisoa, Thomas Rimmelé, François Mallet, Alain Lepape, Julien Textoris, Guillaume Monneret
T lymphocyte alterations are central to sepsis pathophysiology, whereas related mechanisms remain poorly understood. We hypothesized that metabolic alterations could play a role in sepsis-induced T lymphocyte dysfunction. Samples from septic shock patients were obtained at day 3 and compared with those from healthy donors. T cell metabolic status was evaluated in the basal condition and after T cell stimulation. We observed that basal metabolic content measured in lymphocytes by nuclear magnetic resonance spectroscopy was altered in septic patients...
July 19, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28724097/influence-of-long-term-application-of-feedlot-manure-amendments-on-water-repellency-of-a-clay-loam-soil
#7
Jim J Miller, Bruce W Beasley, Paul Hazendonk, Craig F Drury, David S Chanasyk
Long-term application of feedlot manure to cropland may increase the quantity of soil organic carbon (C) and change its quality, which may influence soil water repellency. The objective was to determine the influence of feedlot manure type (stockpiled vs. composted), bedding material (straw [ST] vs. woodchips [WD]), and application rate (13, 39, or 77 Mg ha) on repellency of a clay loam soil after 17 annual applications. The repellency was determined on all 14 treatments using the water repellency index ( index), the water drop penetration time (WDPT) method, and molarity of ethanol (MED) test...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28724086/long-term-changes-in-grassland-soil-phosphorus-with-fertilizer-application-and-withdrawal
#8
Barbara J Cade-Menun, Donnacha G Doody, Corey W Liu, Catherine J Watson
Long-term phosphorus (P) applications can increase soil P concentrations in excess of agronomic optima, posing a risk to water quality. Once fertilization stops, however, it may take time for soil P concentrations to decline. Whereas P fertilization adds orthophosphate, little is known about changes in other soil P forms during P buildup and drawdown. This study examined changes in P pools (total P, Olsen P, Mehlich P, and water-extractable P) and P forms determined by P-nuclear magnetic resonance spectroscopy (P-NMR) in grazed grassland plots from Northern Ireland...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28724045/spinal-tumors-in-children
#9
Andrei Fernandes Joaquim, Enrico Ghizoni, Marcelo Gomes Cordeiro Valadares, Simone Appenzeller, Simone Dos Santos Aguiar, Helder Tedeschi
Introduction: Spinal tumors are rare in the pediatric population, presenting many specific peculiarities when compared to adults. We have performed a broad narrative review to describe the most common spinal tumors in children, discussing their main characteristics and management options. Method: The authors have performed an extensive review of the peer-reviewed literature addressing the aforementioned objectives. Results: Multimodality radiological studies (plain films, 3D computed tomography scan and magnetic resonance imaging) are necessary for proper evaluation and differential diagnosis of spinal tumors in children...
May 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28723250/application-of-molecular-dynamic-simulation-to-study-food-proteins-a-review
#10
Ashutosh Singh, Sai Kranthi Vanga, Valerie Orsat, Vijaya Raghavan
This review presents an overview of the application of molecular dynamic simulation to study food proteins. Processing of food using thermal, chemical, radiation, electromagnetic and mechanical techniques subject its macromolecular bio-components such as carbohydrates and proteins to extreme heat, ionic strength, pH and mechanical deformation. These processing factors affect protein's functional properties such as emulsification, dough formation, gelation, etc. which are associated with changes in their structure...
July 19, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28721737/a-new-water-soluble-nano-micelle-through-self-assembly-pectin-curcumin-conjugates-preparation-characterization-and-anti-cancer-activity-evaluation
#11
Feng Bai, Jiajing Diao, Ying Wang, Shixin Sun, Hongmei Zhang, Yunyun Liu, Yanqing Wang, Jian Cao
Curcumin is a dominating active component of curcuma longa and has been studied widely because of its prominent biological activities. Extremely low aqueous solubility, stability and bioavailability of curcumin limits its application in the field of medicine. In this study, we developed pectin-curcumin (PEC-CCM) conjugates which could self-assemble water-soluble nano-micelles in aqueous solution. The structure of PEC-CCM conjugates was characterized by ultraviolet and visible spectra (UV-Vis), fluorescence spectra, Fourier transform infrared (FTIR) spectroscopy and 1H nuclear magnetic resonance (NMR) spectroscopy...
July 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28721203/evaluation-of-mineral-oil-saturated-hydrocarbons-mosh-and-mineral-oil-aromatic-hydrocarbons-moah-in-pure-mineral-hydrocarbon-based-cosmetics-and-cosmetic-raw-materials-using-1-h-nmr-spectroscopy
#12
Dirk W Lachenmeier, Gerd Mildau, Anke Krause, Gerhard Marx, Stephan G Walch, Andrea Hartwig, Thomas Kuballa
Mineral hydrocarbons consist of two fractions, mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH). MOAH is a potential public health hazard because it may include carcinogenic polycyclic compounds. In the present study, 400 MHz nuclear magnetic resonance (NMR) spectroscopy was introduced, in the context of official controls, to measure MOSH and MOAH in raw materials or pure mineral hydrocarbon final products (cosmetics and medicinal products). Quantitative determination (qNMR) has been established using the ERETIC methodology (electronic reference to access in vivo concentrations) based on the PULCON principle (pulse length based concentration determination)...
2017: F1000Research
https://www.readbyqxmd.com/read/28721043/preparation-of-exenatide-loaded-linear-poly-ethylene-glycol-brush-poly-l-lysine-block-copolymer-potential-implications-on-diabetic-nephropathy
#13
Fei Tong
The poly(ethylene glycol)-b-brush poly(l-lysine) polymer (PEG-b-(PELG50-g-PLL3)) was synthesized and evaluated as a nanocarrier for prolonging delivery of exenatide through the abdominal subcutaneous injection route. The isoelectric point of exenatide was 4.86, and exenatide could combine with PEG-b-(PELG50-g-PLL3) polymers via electrostatic interactions at pH 7.4. This polymer was a good candidate for achieving prolonged drug delivery for exenatide, considering its high molecular weight. Besides the physicochemical characterization of the polymer, in vitro and in vivo applications were researched as a sustained exenatide delivery system...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28720968/technological-quality-of-dough-and-breads-from-commercial-algarroba-wheat-flour-blends
#14
M J Correa, M V Salinas, B Carbas, C Ferrero, C Brites, M C Puppo
Algarroba flour is used to supplement lysine-limiting systems such as wheat flour due to its amino acidic composition. The effects of adding up to 30% of this flour to wheat flour (W-A30) on dough characteristics and breadmaking performance were studied. Dough rheology was tested by farinograph, oscillatory rheometry and texture profile analyses. Molecular mobility was evaluated by nuclear magnetic resonance, and thermal properties were analyzed by differential scanning calorimetry and viscoamylograph studies...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28719906/a-common-ancestral-asn242ser-mutation-in-tmem67-identified-in-multiple-iranian-families-with-joubert-syndrome
#15
MohammadReza Dehghani, Majid Mojarad, Ehsan Ghayoor Karimiani, Mohammad Yahya Vahidi Mehrjardi, Afsaneh Sahebalzamani, Farah Ashrafzadeh, Mehran Beiraghi Toosi, Atiyeh Eslahi, Najmeh Ahangari, Seyed Mojtaba Yassini, Afsaneh Hassanbeigi, Azam Rasti, Seyed Mehdi Kalantar, Reza Maroofian
BACKGROUND: Joubert syndrome (JS) is a clinically and genetically heterogeneous group of rare neurodevelopmental disorder characterised by peculiar midbrain-hindbrain malformation, known as the "molar tooth" sign. JS can manifest a broad range of signs and symptoms. The most common features of JS are hypotonia, ataxia, developmental delay/intellectual disability, abnormal eye movements, and neonatal breathing abnormalities. To date, 29 genes have been shown to cause JS. METHODS: We employed whole-genome single nucleotide polymorphism genotyping in a group of Iranian families with JS and Sanger sequencing of a known mutation associated with JS located in a single homozygous regions shared by affected members of the families...
July 19, 2017: Public Health Genomics
https://www.readbyqxmd.com/read/28719814/enantiomeric-separation-of-seven-%C3%AE-agonists-by-nace-study-of-chiral-selectivity-with-diacetone-d-mannitol-boric-acid-complex
#16
Lili Lv, Lijuan Wang, Jun Li, Yajun Jiao, Shengnan Gao, Jiachang Wang, Hongyuan Yan
A rapid and effective nonaqueous capillary electrophoresis (NACE)-ultraviolet (UV) method was developed for the enantiomeric separation of seven β-agonists. Diacetone-d-mannitol-boric acid complex was used as a new chiral selector. It was in situ synthesized by the reaction of diacetone-d-mannitol and boric acid in methanol medium containing triethylamine. The effects of diacetone-d-mannitol, boric acid, and triethylamine concentrations on the enantioseparation were carefully investigated. Under the optimized conditions, baseline enantioseparation could be obtained for six of the tested β-agonists within 12min...
July 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28717975/development-of-a-novel-polymeric-nanocomposite-complex-for-drugs-with-low-bioavailability
#17
Mduduzi N Sithole, Yahya E Choonara, Lisa C du Toit, Pradeep Kumar, Thashree Marimuthu, Pierre P D Kondiah, Viness Pillay
Semi-synthetic biopolymer complex (SSBC) nanoparticles were investigated as a potential oral drug delivery system to enhance the bioavailability of a poorly water-soluble model drug acyclovir (ACV). The SSBCs were prepared from cross-linking of hydroxyl groups on hyaluronic acid (HA) with poly(acrylic acid) (PAA) resulting in ether linkages. Thereafter, conjugation of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) onto HA-PAA was accomplished using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)-promoted coupling reaction...
July 17, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28715967/hyperthermia-enhanced-targeted-drug-delivery-using-magnetic-resonance-guided-focussed-ultrasound-a-pre-clinical-study-in-a-genetic-model-of-pancreatic-cancer
#18
Navid Farr, Yak-Nam Wang, Samantha D'Andrea, Frank Starr, Ari Partanen, Kayla M Gravelle, Jeannine S McCune, Linda J Risler, Stella G Whang, Amy Chang, Sunil R Hingorani, Donghoon Lee, Joo Ha Hwang
PURPOSE: The lack of effective treatment options for pancreatic cancer has led to a 5-year survival rate of just 8%. Here, we evaluate the ability to enhance targeted drug delivery using mild hyperthermia in combination with the systemic administration of a low-temperature sensitive liposomal formulation of doxorubicin (LTSL-Dox) using a relevant model for pancreas cancer. MATERIALS AND METHODS: Experiments were performed in a genetically engineered mouse model of pancreatic cancer (KPC mice: LSL-Kras(G12D/+); LSL-Trp53(R172H/+); Pdx-1-Cre)...
July 17, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28715188/understanding-microscopic-behavior-of-the-mixture-of-ionic-liquid-ethylene-glycol-lithium-salt-through-time-resolved-fluorescence-nuclear-magnetic-resonance-nmr-and-electron-paramagnetic-resonance-epr-studies
#19
Ranu Satish Dhale, Prabhat Kumar Sahu, Moloy Sarkar
The present study has been undertaken with an aim to find out the suitability of a binary solvent system, comprising an ionic liquid, 1-(2-Hydroxyethyl)-3-methylimidazolium bis (trifluoromethylsulfonyl)imide ([OHEMIM][NTf2]) and ethylene glycol (EG), towards lithium ion battery applications. For this purpose, the behavior in terms of structure, intermolecular interaction and dynamics of several solvent systems, [OHEMIM][NTf2], [OHEMIM][NTf2]-LiNTf2(lithium bis(trifluoromethylsulfonyl)imide), [OHEMIM][NTf2]-EG and [OHEMIM][NTf2]-EG-LiNTf2 are investigated by carrying out steady state and time-resolved fluorescence, nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) measurements...
July 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28715023/amorphous-boron-nanorod-as-an-anode-material-for-lithium-ion-batteries-at-room-temperature
#20
Changjian Deng, Miu Lun Lau, Heather M Barkholtz, Haiping Xu, Riley Parrish, Meiyue Olivia Xu, Tao Xu, Yuzi Liu, Hao Wang, Justin G Connell, Kassiopeia A Smith, Hui Xiong
We report an amorphous boron nanorod anode material for lithium-ion batteries prepared through smelting non-toxic boron oxide in liquid lithium. Boron in theory can provide capacity as high as 3099 mA h g(-1) by alloying with Li to form B4Li5. However, experimental studies of the boron anode have been rarely reported for room temperature lithium-ion batteries. Among the reported studies the electrochemical activity and cycling performance of the bulk crystalline boron anode material are poor at room temperature...
July 17, 2017: Nanoscale
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