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German A Estrada-Bonilla, Cintia M Lopes, Ademir Durrer, Paulo R L Alves, Nicolle Passaglia, Elke J B N Cardoso
Sugarcane processing generates a large quantity of residues, such as filter cake and ashes, which are sometimes composted prior to their amendment in soil. However, important issues still have to be addressed on this subject, such as the description of bacterial succession that occurs throughout the composting process and the possibilities of using phosphate-solubilizing bacteria (PSB) during the process to improve phosphorus (P) availability in the compost end product. Consequently, this study evaluated the bacterial diversity and P dynamics during the composting process when inoculated with Pseudomonas aeruginosa PSBR12 and Bacillus sp...
June 7, 2017: Systematic and Applied Microbiology
Julia H Seeliger, Ute U Botzenhart, Tomasz Gedrange, Karol Kozak, Lukas Stepien, Monika Machoy
AIM: The aim of this study was a comparison of shear bond strength (SBS) on tooth enamel of different primers combined with the adhesive paste Transbond XT. MATERIALS AND METHODS: Forty bovine teeth were used in order to create 40 test blocks. The blocks were divided into four groups of 10 blocks each: group A - sample primer (SP); group B - Opal Seal (OS); group C - Transbond Plus SEP (TSEP); group D - Transbond XT Primer (TXT). After surface preparation and application of the primer, respectively, two stainless steel brackets were fixed on each tooth by using Transbond XT...
June 22, 2017: Biomedizinische Technik. Biomedical Engineering
D W Li, Q M Zou, X Huang, H Rabiee Golgir, K Keramatnejad, J F Song, Z Y Xiao, L S Fan, X Hong, L Jiang, J F Silvain, S Sun, Y F Lu
It is known that defects strongly influence the properties of two-dimensional (2D) materials. The controlled creation and removal of defects can be utilized to tailor the optical and electronic responses of these 2D materials for optoelectronic and nanoelectronic applications. In this study, we developed an efficient approach to reversibly control the defect states in mechanically exfoliated graphene and molybdenum disulfide (MoS2) monolayers. The defects were created by aluminium oxide (Al2O3) plasmas and removed by moderate thermal annealing at up to 300 °C...
June 22, 2017: Nanoscale
Lian Xu, Ji-Quan Sun, Li-Juan Wang, Zhi-Wei Gao, Li-Zhu Sun, Xiao-Lei Wu
A Gram-staining-negative, non-motile, non-spore-forming bacterium designated Y3L14T was isolated from the saline-alkaline soil of a farmland, Inner Mongolia, northern China. Strain Y3L14T could grow at 10-40 °C (optimally at 30 °C), pH 6.0-10.0 (optimally at pH 8.0), and in the presence of 0-6.0 % (w/v) NaCl (optimally with 0-2.0 %). Phylogenetic analysis based on the 16S rRNA gene and DNA gyrase subunit B (gyrB) gene sequences revealed that strain Y3L14T clustered with strains belonging to the genus Sphingobacterium, sharing the highest 16S rRNA gene sequence similarity with Sphingobacterium lactis WCC 4512T (94...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
Stephen J Freedland, William J Aronson
Toward development of a precision medicine framework for metastatic, castration-resistant prostate cancer (mCRPC), we established a multi-institutional clinical sequencing infrastructure to conduct prospective whole-exome and transcriptome sequencing of bone or soft tissue tumor biopsies from a cohort of 150 mCRPC affected individuals. Aberrations of AR, ETS genes, TP53, and PTEN were frequent (40%-60% of cases), with TP53 and AR alterations enriched in mCRPC compared to primary prostate cancer. We identified new genomic alterations in PIK3CA/B, R-spondin, BRAF/RAF1, APC, β-catenin, and ZBTB16/PLZF...
June 13, 2017: Urologic Oncology
Ahmed H Afifi, Ippei Kagiyama, Ahmed H El-Desoky, Hikaru Kato, Remy E P Mangindaan, Nicole J de Voogd, Nagwa M Ammar, Mohammed S Hifnawy, Sachiko Tsukamoto
Three new furanosesterterpene tetronic acids, sulawesins A-C (1-3), were isolated from a Psammocinia sp. marine sponge, along with the known compounds ircinins-1 (4) and -2 (5). Although ircinins-1 and -2 were previously isolated as (+)- or (-)-enantiomers from marine sponges, we isolated them as enantiomeric mixtures. Sulawesins A and B possess a new carbon skeleton with a 5-(furan-3-yl)-4-hydroxycyclopent-2-enone moiety and were also found to be diastereomeric mixtures of four isomers by an HPLC analysis with a chiral-phase column...
June 16, 2017: Journal of Natural Products
Philip P Adams, Carlos Flores Avile, Mollie W Jewett
Knowledge of the transcriptional responses of vector-borne pathogens at the vector-pathogen interface is critical for understanding disease transmission. Borrelia (Borreliella) burgdorferi, the causative agent of Lyme disease in the United States, is transmitted by the bite of infected Ixodes sp. ticks. It is known that B. burgdorferi has altered patterns of gene expression during tick acquisition, persistence and transmission. Recently, we and others have discovered in vitro expression of RNAs found internal, overlapping, and antisense to annotated open reading frames in the B...
2017: Frontiers in Cellular and Infection Microbiology
Leki Wangchu, Dobiam Narba, Michi Yassa, Amit Tripathi
AIM: This study was a part of an ongoing parasitological survey to investigate the health status of hill stream fish in river systems of Arunachal Pradesh - A global biodiversity hotspot. MATERIALS AND METHODS: During the 2013/2015, 18 live specimens of Barilius barna (Cyprinidae) were captured from the local rivers of Arunachal Pradesh and examined for parasitic monogenoids. These fish, with their flatworms, were immediately fixed in hot (60°C) 4% formalin for later examination...
May 2017: Veterinary World
S T Bino Sundar, B Dhivya, G Jyothimol, R Anandaraja, P Ramesh, C Balachandran, M Thangapandiyan, C Soundararajan, S Gomathinayagam, Bhaskaran Ravi Latha, T J Harikrishnan
Onchocerca gibsoni subcutaneous nodules in four cross bred Jersey cows aged 5-6 years brought for post mortem with calcified and caseated skin nodules in the lateral flank region is reported. Examination and dissection of these nodules revealed that they were "worm nests" of Onchocerca sp. of filarid nematodes. The worm nests were carefully dissected and filarid worms were extracted out. Multiple numbers of worms were recovered from each nodule ranging from 15 to 20. Female worms were found inside the worm nests and were found to be filled with microfilariae...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Yanmin Lu, Hongyan Lu, Jiangyan Liu, Ming Chang, Qiuxia Wang, Wei Tang
Objective To explore the effect of hyperoxia on the expressions of CCAAT enhancer binding protein α (C/EBPα) and pulmonary surfactant proteins (SP-A, SP-B, SP-C, SP-D) and their correlations in primary type II alveolar epithelial cells (AECIIs) from premature rats. Methods AECIIs were divided into an air control group and a hyperoxia model group. The cells of the two groups were respectively exposed toair and 950 mL/L O2. The cells were harvested at 24, 48, and 72 hours after exposure. Inverted phase-contrast microscope was used to observe morphological changes of the cells...
June 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Martin A O'Reilly, Darragh Whelan, Tomás E Ward, Eamonn Delahunt, Brian Caulfield
BACKGROUND: The barbell squat is a popularly used lower limb rehabilitation exercise. It is also an integral exercise in injury risk screening protocols. To date athlete/patient technique has been assessed using expensive laboratory equipment or subjective clinical judgement; both of which are not without shortcomings. Inertial measurement units (IMUs) may offer a low cost solution for the objective evaluation of athlete/patient technique. However, it is not yet known if global classification techniques are effective in identifying naturally occurring, minor deviations in barbell squat technique...
June 14, 2017: Methods of Information in Medicine
Erika Santos, Clélia M C DA Rocha, Edivaldo Jr Gomes, Paulo Fontoura
Three new tardigrade species, Batillipes brasiliensis sp. nov., Batillipes dandarae sp. nov. and Batillipes potiguarensis sp. nov., are described from shallow subtidal sediments of the Brazilian coast. B. brasiliensis sp. nov. and B. dandarae sp. nov. have toes 3 and 4 on leg IV different in length, so they can be included in the D group of species, while B. potiguarensis sp. nov., with toes 3 and 4 on leg IV equal in length belong to the A group. Batillipes brasiliensis sp. nov. is characterized by having an ala-like caudal expansion; cuticular projections on the coxal region of legs I-III, and lateral projections...
March 16, 2017: Zootaxa
Yu Mei Xu, Zeng Qi Zhao, Kerrie A Davies, Jian Ming Wang
This paper describes a new species of Tripylella and gives a new record of Tripyla (family Tripylidae) in Australia, and provides a key to species in the genus Tripylella. Tripylella australis sp. nov. is characterized by having females with a long body (1804-1832 μm), a=41-42, b= 4.4-4.5, c=10.3-11.3, c'=5.4-6.4 and V=51-54%, outer labial setae 13-14 μm long and cephalic setae 3.7-4.5 μm long in one whorl, dorsal tooth posterior to two subventral teeth, tail tapering uniformly, and four single caudal setae on the dorsal part of the tail...
April 3, 2017: Zootaxa
Malte Seehausen, Günther Theischinger
Nososticta impercepta sp. nov. is described and illustrated (holotype ♂:, Niki-Niki [South Central Timor Regency], Timor, Indonesia, C.B. Haniel leg., Coll. Ris, No. 2477; deposited at the Forschungsinstitut und Naturmuseum Senckenberg, Frankfurt am Main, Germany). An illustrated key for the species of Nososticta known from the Lesser Sunda Islands is given. The occurrence of N. selysi in Timor is considered to be doubtful. Nososticta emphyla is newly recorded from Sumbawa Island.
April 5, 2017: Zootaxa
Paulo Vilela Cruz, Frederico Falcão Salles, Neusa Hamada
The first phylogenetic study of Callibaetis Eaton indicated an unrevealed diversity in this genus, including four new species named as Callibaetis sp. A, B, C and D, and the undescribed nymph of Callibaetis (Abaetetuba) fasciatus (Pictet). Given that neither the nymph of C. (Ab.) fasciatus nor the four new species received a proper description, the objective of this study is to describe them. The nymph of C. (Ab.) fasciatus differs from the others species in the subgenus by the presence of one row of setae on basal part of inner-dorsal row of maxilla and metanotum without spines; Callibaetis sp...
April 5, 2017: Zootaxa
Anton V Volynkin, Karel Černý
Taxa of the Barsine delineata (Walker, 1854) species-complex are examined, the synonymy of Ammatho figuratus Walker, 1855, Cyme chinensis C. Felder & R. Felder, 1862 and Ammatho fuscescens Butler, 1877 with Hypoprepia? delineata Walker, 1854 is revised. The new synonymy is introdiced: Barsine delineata (Walker, 1854) = Miltochrista dimidiata Fang, 1991, syn. nov. A new species, Barsine sublucana Volynkin & Černý, sp. nov. is described from northern Thailand. Adults, male and female genitalia of B. delineata and B...
April 27, 2017: Zootaxa
R Ananda Raja, R Sridhar, C Balachandran, A Palanisammi, S Ramesh, K Nagarajan
A pathobiological study was conducted using Vibrio parahaemolyticus (VP) strain isolated from vibriosis affected shrimp (Penaeus vannamei) farms in Kancheepuram and Thiruvallur districts of Tamil Nadu during August 2014 to February 2015. The isolate was identified based on the morphological, physiological, biochemical and molecular characters. LD50 value with intramuscular injection was determined as 2.6 × 10(4) cfu/shrimp and sequential pathology was studied giving 6.1 × 10(3) cfu/shrimp (LD25). Total plate count (TPC) and total Vibrio count (TVC) in water, pond sediment, haemolymph, muscle, HP and gut were found significantly (P < 0...
June 9, 2017: Fish & Shellfish Immunology
Wilmar Maarisit, Delfly B Abdjul, Hiroyuki Yamazaki, Hajime Kato, Henki Rotinsulu, Defny S Wewengkang, Deiske A Sumilat, Magie M Kapojos, Kazuyo Ukai, Michio Namikoshi
Three new dimeric 3-alkyl pyridinium alkaloids, named haliclocyclamines A-C (1-3), were isolated together with five known congeners, cyclostellettamines A (4), B (5), C (6), E (7), and F (8), from the Indonesian marine sponge Haliclona sp. The structures of 1-3 were assigned based on their spectroscopic data (1D and 2D NMR, HRFABMS, ESIMS/MS, UV, and IR). Compounds 1-8 exhibited antimicrobial activities against Mycobacterium smegmatis with inhibition zones of 17, 10, 13, 14, 8, 8, 12, and 12mm, respectively, at 10μg/disc...
May 23, 2017: Bioorganic & Medicinal Chemistry Letters
Abir Ben Bacha, Ikram Jemel, Nadine M S Moubayed, Imen Ben Abdelmalek
Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was selected as a protease inhibitor source. The maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 M phosphate buffer (pH 7.0) and isolated from the mature leaves...
June 2017: 3 Biotech
Chetan C Gaonkar, Wiebe H C F Kooistra, Carina B Lange, Marina Montresor, Diana Sarno
The diatom genus Chaetoceros is one of the most abundant and diverse phytoplankton in marine and brackish waters worldwide. Within this genus, Chaetoceros socialis Lauder has been cited as one of the most common species. However, recent studies from different geographic areas have shown the presence of pseudo-cryptic diversity within the C. socialis complex. Members of this complex are characterized by curved chains (primary colonies) aggregating into globular clusters, where one of the four setae of each cell curves towards the center of the cluster and the other three orient outwards...
June 7, 2017: Journal of Phycology
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