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https://www.readbyqxmd.com/read/28229261/revision-of-neolebouria-gibson-1976-digenea-opecoelidae-with-trilobovarium-n-g-for-species-infecting-tropical-and-subtropical-shallow-water-fishes
#1
Storm B Martin, Scott C Cutmore, Thomas H Cribb
A new opecoelid trematode is reported from fishes of the Lethrinidae, Lutjanidae and Nemipteridae off Lizard Island on the northern Great Barrier Reef, Australia. The new species keys to Neolebouria Gibson, 1976 and shows strong similarity to several species of that genus, but is not consistent with the type-species, N. georgiensis Gibson, 1976, or others known from temperate/polar and/or deep-sea fishes. The new species is also phylogenetically distant from N. lanceolata (Price, 1934) Reimer, 1987, the only representative of the genus for which molecular data are available...
February 22, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28224230/newly-isolated-bacillus-sp-g51-from-patagonian-wool-produces-an-enzyme-combination-suitable-for-felt-resist-treatments-of-organic-wool
#2
Martín S Iglesias, Cynthia Sequeiros, Sebastián García, Nelda L Olivera
Bacteria from Patagonian Merino wool were isolated to assess their wool-keratinolytic activity and potential for felt-resist treatments. Strains from Bacillus, Exiguobacterium, Deinococcus, and Micrococcus produced wool-degrading enzymes. Bacillus sp. G51 showed the highest wool-keratinolytic activity. LC-MS/MS analysis revealed that G51 secreted two serine proteases belonging to the peptidase family S8 (MEROPS) and a metalloprotease associated with Bacillolysin, along with other enzymes (γ-glutamyltranspeptidase and dihydrolipoyl dehydrogenases) that could be involved in reduction of keratin disulfide bonds...
February 21, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28221717/cerebral-and-lung-effects-of-a-new-generation-synthetic-surfactant-with-sp-b-and-sp-c-analogs-in-preterm-lambs
#3
Carmen Rey-Santano, Victoria E Mielgo, Xabier Murgia, Miguel Angel Gomez-Solaetxe, Fabrizio Salomone, Federico Bianco, Nicola Pelizzi, Begoña Loureiro, Jon López de Heredia Y Goya
BACKGROUND: Though natural surfactants (SF) are clinically superior to protein-free synthetic preparations, CHF-5633, a synthetic SF containing SP-B and SP-C analog peptides is a potential alternative to natural SF for treating neonatal respiratory distress syndrome (RDS). Nevertheless, information is lacking regarding the safety of this new treatment for the neonatal brain. We sought to compare the cerebral and pulmonary effects of this new synthetic surfactant (CHF5633) with those of natural porcine surfactant (Cursosurf) in premature lambs with RDS...
February 21, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28220476/response-of-trabecular-bone-thyroid-c-and-follicular-cells-to-synthetic-salmon-calcitonin-in-middle-aged-orchidectomized-male-rats
#4
Branko Filipović, Branka Šošić-Jurjević, Vladimir Ajdžanović, Jasmina Živanović, Nataša Ristić, Svetlana Trifunović, Verica Milošević
In contrast to studies in women, male osteoporosis is poorly understood and strictly related to advancing age. Among the first antiresorptive substances used in the prevention and treatment of osteoporosis is calcitonin (CT), a hypocalcemic hormone that potently inhibits osteoclastic bone resorption. Natural CT is produced and secreted by thyroid C-cells. The other endocrine population of thyroid cells produces thyroid hormones (TH), which also affect bone turnover. The aim of this study was to evaluate the influence of salmon CT on trabecular bone microarchitecture with special reference to effects on the structure and function of both CT- and TH-producing thyroid cells in orchidectomized (Orx) middle-aged rats...
February 20, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28211474/dissection-of-genomic-features-and-variations-of-three-pathotypes-of-puccinia-striiformis-through-whole-genome-sequencing
#5
Kanti Kiran, Hukam C Rawal, Himanshu Dubey, R Jaswal, Subhash C Bhardwaj, P Prasad, Dharam Pal, B N Devanna, Tilak R Sharma
Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici, is one of the important diseases of wheat. We used NGS technologies to generate a draft genome sequence of two highly virulent (46S 119 and 31) and a least virulent (K) pathotypes of P. striiformis from the Indian subcontinent. We generated ~24,000-32,000 sequence contigs (N50;7.4-9.2 kb), which accounted for ~86X-105X sequence depth coverage with an estimated genome size of these pathotypes ranging from 66.2-70.2 Mb. A genome-wide analysis revealed that pathotype 46S 119 might be highly evolved among the three pathotypes in terms of year of detection and prevalence...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28211262/superlight-and-superflexible-three-dimensional-semiconductor-frameworks-a-x%C3%A2-y-4-a-si-ge-x-y-c-b-n-with-tunable-optoelectronic-and-mechanical-properties-from-first-principles
#6
Xinrui Cao, Xiao-Fei Li, Zi-Zhong Zhu
Silicon carbide materials as the leading wide-bandgap semiconductor holds significant importance in semiconductor technologies. Here, diamond-like three-dimensional (3D) materials with low density but high elasticity properties have been designed from first-principles calculations. They are porous single-crystalline materials composed of sp3-hybridized Si (or Ge) and sp-type C≡C (or B≡N) linear moieties, while their stabilities are found to be comparable to the recently prepared SiC4 materials. Moreover, we find that such wide-bandgap semiconductors have strong absorption in a wide range of UV region and exhibit superlight, superflexible, and incompressible mechanical properties, and their optoelectronic and mechanical properties can be well tuned via structural modifications...
February 17, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28208722/zunyimycins-b-and-c-new-chloroanthrabenzoxocinones-antibiotics-against-methicillin-resistant-staphylococcus-aureus-and-enterococci-from-streptomyces-sp-fjs31-2
#7
Yuhong Lü, Meiyun Shao, Yinyin Wang, Shengyan Qian, Miao Wang, Yingquan Wang, Xiaoqian Li, Yuxin Bao, Chengmin Deng, Changwu Yue, Daishun Liu, Ning Liu, Minghao Liu, Ying Huang, Zehui Chen, Yonglin Hu
This study performed an optimization of the fermentation conditions to activate the expression of the zunyimycin family biosynthesis genes of the zunyimycin-producing streptomycetes strain Streptomyces sp. FJS31-2. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of the zunyimycin derivatives, namely, 31-2-7 and 31-2-8, accompanied with three known anthrabenzoxocinones family members of zunyimycin A, BE24566B, and chloroanthrabenzoxocinone. Their structures were elucidated by NMR, HRESIMS, IR, UV, and CD...
February 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28208607/induction-of-diverse-bioactive-secondary-metabolites-from-the-mangrove-endophytic-fungus-trichoderma-sp-strain-307-by-co-cultivation-with-acinetobacter-johnsonii-strain-b2
#8
Liuhong Zhang, Shah Iram Niaz, Dilfaraz Khan, Zhen Wang, Yonghong Zhu, Haiyun Zhou, Yongcheng Lin, Jing Li, Lan Liu
Two new sesquiterpenes, microsphaeropsisin B (1) and C (2), and two new de-O-methyllasiodiplodins, (3R, 7R)-7-hydroxy-de-O-methyllasiodiplodin (4) and (3R)-5-oxo-de-O-methyllasiodiplodin (5), together with one new natural product (6) and twelve known compounds (3, 7-17), were isolated from the co-cultivation of mangrove endophytic fungus Trichoderma sp. 307 and aquatic pathogenic bacterium Acinetobacter johnsonii B2. Their structures, including absolute configurations, were elucidated by extensive analysis of spectroscopic data, electronic circular dichroism, Mo₂(AcO)₄-induced circular dichroism, and comparison with reported data...
February 10, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28207271/synthesis-of-benzyl-allyl-and-allenyl-boronates-via-copper-catalyzed-borylation-of-alcohols
#9
Lujia Mao, Kálmán J Szabó, Todd B Marder
Alcohols are among the most abundant and readily available organic feedstocks in industrial processes. The direct catalytic functionalization of sp(3) C-O bonds of alcohols remains the main challenge in this field. Here, we report a copper-catalyzed synthesis of benzyl-, allyl-, and allenyl-boronates from benzylic, allylic, and propargylic alcohols, respectively. This protocol exhibits a broad reaction scope (40 examples) and high efficiency (up to 95% yield) under mild conditions, including for the preparation of secondary allylic boronates...
February 16, 2017: Organic Letters
https://www.readbyqxmd.com/read/28198499/cloning-and-expression-of-class-i-chitinases-in-hami-melon-after-penicillium-infection
#10
F Y Jiang, B B Shui, F X Tang, C H Shan
Chitinases are important disease-related proteins that play critical roles in plant defense against disease. To investigate the function of chitinases in the resistance of Hami melon to Penicillium infection, the gene encoding chitinases, HmCHT-2, was cloned and RT-PCR was used to measure expression levels of HmCHT-2. When the Hami melon was infected by Penicillium sp after 0, 12, 36, 48, 60, and 72 h. The results showed that comparing to the control group, the time of expression levels reaching to the peak delayed and the expression levels maintained at a significantly high level for a longer time...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28197131/bacillus-spp-isolated-from-puba-as-a-source-of-biosurfactants-and-antimicrobial-lipopeptides
#11
Karla J Perez, Jaime Dos Santos Viana, Fernanda C Lopes, Jamile Q Pereira, Daniel M Dos Santos, Jamil S Oliveira, Renata V Velho, Silvia M Crispim, Jacques R Nicoli, Adriano Brandelli, Regina M D Nardi
Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28196973/two-new-polyketides-from-the-ascomycete-fungus-leptosphaeria-sp
#12
Jie Lin, Renlei Wang, Guohua Xu, Zhengfeng Ding, Xueshen Zhu, Erwei Li, Ling Liu
Leptosphaerins H and I (1 and 2), two new xanthone derivatives, and six known compounds, leptosphaerin F (3), monodictysin B (4), norlichexanthone (5), leptosphaerin D (6), moniliphenone (7) and emodinbianthrone (8) have been isolated from a scale-up fermentation of the ascomycete fungus Leptosphaeria sp. Their structures were primarily elucidated by interpretation of NMR spectroscopic data. The absolute configuration of 1 was assigned using the modified Mosher method, whereas that of C-8a in 2 was determined via the CD data...
February 15, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28192446/range-extension-for-the-common-dolphin-delphinus-sp-to-the-colombian-caribbean-with-taxonomic-implications-from-genetic-barcoding-and-phylogenetic-analyses
#13
Nohelia Farías-Curtidor, Dalia C Barragán-Barrera, Paula Alejandra Chávez-Carreño, Cristina Jiménez-Pinedo, Daniel M Palacios, Dalila Caicedo, Fernando Trujillo, Susana Caballero
The nearest known population of common dolphins (Delphinus sp.) to the Colombian Caribbean occurs in a fairly restricted range in eastern Venezuela. These dolphins have not been previously reported in the Colombian Caribbean, likely because of a lack of study of the local cetacean fauna. We collected cetacean observations in waters of the Guajira Department, northern Colombia (~11°N, 73°W) during two separate efforts: (a) a seismic vessel survey (December 2009-March 2010), and (b) three coastal surveys from small boats (May-July 2012, May 2013, and May 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28187664/-i-uroptychus-i-i-nitidus-i-a-milne-edwards-1880-and-related-species-crustacea-decapoda-anomura-chirostylidae-from-the-western-atlantic
#14
Keiji Baba, Mary K Wicksten
Eight species of squat lobsters of the genus Uroptychus are reported from the western Atlantic based on the collections of the Museum of Comparative Zoology at Harvard, the Smithsonian Institution and Texas A&M University. Uroptychus nitidus (A. Milne-Edwards, 1880) is reviewed and redescribed, with a syntype taken at Blake Station 200 off Martinique designated as the lectotype. Uroptychus alphonsei n. sp. is named for U. nitidus variety C Chace, 1942, U. fenneri n. sp. for U. nitidus variety A Chace, 1942, and U...
January 17, 2017: Zootaxa
https://www.readbyqxmd.com/read/28186498/underlying-mechanisms-for-syntrophic-metabolism-of-essential-enzyme-cofactors-in-microbial-communities
#15
Margaret F Romine, Dmitry A Rodionov, Yukari Maezato, Andrei L Osterman, William C Nelson
Many microorganisms are unable to synthesize essential B vitamin-related enzyme cofactors de novo. The underlying mechanisms by which such microbes survive in multi-species communities are largely unknown. We previously reported the near-complete genome sequence of two ~18-member unicyanobacterial microbial consortia that maintain stable membership on defined medium lacking vitamins. Here we have used genome analysis and growth studies on isolates derived from the consortia to reconstruct pathways for biogenesis of eight essential cofactors and predict cofactor usage and precursor exchange in these communities...
February 10, 2017: ISME Journal
https://www.readbyqxmd.com/read/28184966/recombinant-bacillus-thuringiensis-subsp-kurstaki-hd73-strain-that-synthesizes-cry1ac-and-chimeric-chia74%C3%A2-sp-chitinase-inclusions
#16
Karen S González-Ponce, Luz E Casados-Vázquez, Rubén Salcedo-Hernández, Dennis K Bideshi, María C Del Rincón-Castro, José E Barboza-Corona
In this study, the endochitinase chiA74 gene lacking its secretion signal peptide sequence (chiA74∆sp) was fused in frame with the sequence coding for the C-terminal crystallization domain and transcription terminator of cry1Ac. The chimeric gene was expressed under the strong pcytA-p/STAB-SD promoter system in an acrystalliferous Cry(-)B strain of Bacillus thuringiensis and B. thuringiensis subsp. kurstaki HD73. We showed that the chimeric ChiA74∆sp produced amorphous inclusions in both Cry(-)B and HD73...
February 9, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28181397/b-c6-f5-3-catalyzed-chemoselective-defunctionalization-of-ether-containing-primary-alkyl-tosylates-with-hydrosilanes
#17
Indranil Chatterjee, Digvijay Porwal, Martin Oestreich
Catalytic C(sp(3) )-O bond cleavage promoted by B(C6 F5 )3  /Et3 SiH proceeds preferentially with primary tosylates in the presence of primary and secondary silyl ethers and aryl ethers. This reactivity difference enables the chemoselective defunctionalization of several 1,n-diols, and the efficiency of the new procedure is highlighted by the selective deoxygenation of the hydroxymethyl group of an orthogonally protected carbohydrate. Tosylates with an adjacent phenyl group are cleaved with anchimeric assistance...
February 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28180951/bradyrhizobium-centrolobii-and-bradyrhizobium-macuxiense-sp-nov-isolated-from-centrolobium-paraense-grown-in-soil-of-amazonia-brazil
#18
Daniele C Michel, Samuel R Passos, Jean L Simões-Araujo, Alexandre C Baraúna, Krisle da Silva, Marcia M Parma, Itamar S Melo, Sofie E De Meyer, Graham O'Hara, Jerri E Zilli
Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36 °C (optimal 28 °C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity...
February 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28180193/acoustic-radiation-force-impulse-arfi-elastography-for-non%C3%A2-invasive-evaluation-of-hepatic-fibrosis-in-chronic-hepatitis-b-and-c-patients-a-systematic-review-and-meta-analysis
#19
Xiangdong Hu, Lanyan Qiu, Dong Liu, Linxue Qian
AIM: The purpose of the study was to assess the effect of Acoustic Radiation Force Impulse (ARFI) elastography in the diagnosis of liver fibrosis in chronic hepatitis B and C patients through Meta-analysis. MATERIAL AND METHODS: Four databases (PubMed, the Cochrane Library, WanFang data, and CNKI) were searched. The key words were: ("ARFI" or "acoustic radiation force impulse") combined with "liver fibrosis" and ("chronic hepatitis" or "HBV HCV"). Heterogeneity (I2) was assessed, and its source was analyzed through meta-regression...
January 31, 2017: Medical Ultrasonography
https://www.readbyqxmd.com/read/28178978/enhanced-extracellular-production-of-recombinant-proteins-in-escherichia-coli-by-co-expression-with-bacillus-cereus-phospholipase-c
#20
Lingqia Su, Qi Jiang, Lingang Yu, Jing Wu
BACKGROUND: Our laboratory has reported a strategy for improving the extracellular production of recombinant proteins through co-expression with Thermobifida fusca cutinase, which increases membrane permeability via its phospholipid hydrolysis activity. However, the foam generated by the lysophospholipid product makes the fermentation process difficult to control in a fermentor. Phospholipase C (PLC) catalyzes the hydrolysis of phospholipids to produce sn1,2-diacylglycerides and organic phosphate, which do not induce foam formation...
February 8, 2017: Microbial Cell Factories
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