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https://www.readbyqxmd.com/read/28100298/description-of-two-novel-members-of-the-family-erysipelotrichaceae-ileibacterium-valens-gen-nov-sp-nov-and-dubosiella-newyorkensis-gen-nov-sp-nov-from-the-murine-intestine-and-emendation-to-the-description-of-faecalibacterium-rodentium
#1
Laura M Cox, Jiho Sohn, Kerin L Tyrrell, Diane M Citron, Paul A Lawson, Nisha B Patel, Tadasu Iizumi, Guillermo I Perez-Perez, Ellie J C Goldstein, Martin J Blaser
To better characterize murine intestinal microbiota, a large number (187) of Gram-positive staining, rod- and coccoid-shaped facultative and strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene similarity values of 87...
January 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28099573/hematological-and-morphometric-differences-of-blood-cells-from-rheas-rhea-americana-struthioniformes-rheidae-on-two-conservation-farms
#2
S S M Gallo, N B Ederli, F C R Oliveira
Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espírito Santo (ES), and the other in São Carlos, São Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained...
January 12, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28096859/occurrence-and-intensity-of-anisakid-nematode-larvae-in-some-commercially-important-fish-species-in-persian-gulf
#3
Maryam Dadar, Alireza Alborzi, Rahim Peyghan, Milad Adel
BACKGROUND: Anisakid nematodes are common parasites of fish, mammals, fish-eating birds, and reptiles with a worldwide distribution, causing diseases in human, fish and important economic losses. METHODS: A preliminary epidemiological study was carried out on Anisakid nematodes larvae in some commercially important fish species to evaluate the anisakid nematode larvae from greater lizardfish, (Saurida tumbil), Japanese thread fin bream (Nemipterus japonicus), crocodile longtom (Tylosurus crocodilus crocodiles) and longfin trevally (Carangoides armatus) from the Persian Gulf of Iran...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096576/role-of-edaravone-in-managemant-of-septic-peritonitis
#4
Ghada Fouad Elbaradey, Nagat Sayed Elshmaa, Hossam Hodeib
BACKGROUND AND AIMS: Sepsis is a complex rapidly progressive infectious disease that remain a major cause of morbidity and mortality in surgical patients and trauma victims. Edaravone a novel free radical scavenger was approved in 2001 in Japan for treatment of acute cerebral and myocardial infarction. Hence, in this work we attempt to evaluate its role in cases of septic peritonitis (SP). MATERIAL AND METHODS: This is a prospective randomized observer-blinded study carried out in surgical Intensive Care Unit (ICU) after approval by Hospital Ethical Committee...
October 2016: Journal of Anaesthesiology, Clinical Pharmacology
https://www.readbyqxmd.com/read/28087802/cytochrome-c-peroxidase-ccp-is-a-molecular-determinant-of-the-oxidative-stress-response-in-the-extreme-acidophilic-leptospirillum-sp-cf-1
#5
C Zapata, B Paillavil, R Chávez, P Álamos, G Levicán
Bioleaching processes are used to recover metals from sulfidic ores. Biofilm formation on ores is important for bioleaching because the attached microorganisms start the leaching process by concentrating ferric ions in the extracellular matrix. It has been shown that hydrogen peroxide is spontaneously generated on the surface of ores and that it negatively influences the growth and activity of microorganisms. However, the mechanism by which bioleaching microorganisms tolerate exogenous H2O2 as an adaptive trait remains elusive...
January 12, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28087527/floc-formation-reduces-the-ph-stress-experienced-by-microorganisms-living-in-alkaline-environments
#6
C J Charles, S P Rout, K A Patel, S Akbar, A P Laws, B R Jackson, S A Boxall, P N Humphreys
: The survival of microorganisms within a cementitious geological disposal facility for radioactive wastes is heavily dependent on their ability to survive the calcium dominated, hyper-alkaline conditions resulting from the dissolution of the cementitious materials. The present study shows that the formation of flocs, composed of a complex mixture of extracellular polymeric substances (EPS), provides protection against alkaline pH values up to pH 13.0. The flocs were dominated by Alishewanella and Dietzia sp, producing a mannose rich carbohydrate fraction incorporating extracellular DNA, resulting in Ca(2+) sequestration...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28077319/role-of-insulin-like-growth-factor-axis-in-the-bleomycin-induced-lung-injury-in-rats
#7
Lakshmi Kanth Kotarkonda, Ritu Kulshrestha, Krishnan Ravi
BACKGROUND: Alveolar epithelial cell injury has been proposed as a causative factor for the onset and progression of pulmonary fibrosis. However, the role of type II alveolar epithelial cells (AECs) in the epithelial mesenchymal transition (EMT) is controversial. AIMS: The present study performed in rats instilled with bleomycin investigated a) the expressions of the insulin growth factor (IGF-1) and insulin growth factor binding protein 5 (IGFBP-5) and transforming growth factor (TGF-β1) in the type II AECs, b) the role of type II AECs in EMT and extracellular matrix (ECM) formation and, c) the effect of pioglitazone on all the above parameters...
January 7, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28074048/elmenols-c-h-new-angucycline-derivatives-isolated-from-a-culture-of-streptomyces-sp-ifm-11490
#8
Yixizhuoma, Naoki Ishikawa, Mohamed S Abdelfattah, Masami Ishibashi
Chemical investigations of the ethyl acetate extract of Streptomyces sp. IFM 11490 have led to the isolation of six new angucycline metabolites, named elmenols C-H (1-6), along with the previously reported elmonin (7) and elmenols A (8) and B (9). The known LS1924A (10), 6-deoxy-8-methylrabelomycin (11), tetrangulol methyl ether (12) and angucyclinone (13) were additionally identified. The structures of the isolated compounds were elucidated by means of spectroscopic methods including UV, IR, HRESIMS, and 1D and 2D NMR...
January 11, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28073563/genetic-and-toxinological-characterization-of-north-atlantic-strains-of-the-dinoflagellate-ostreopsis-and-allelopathic-interactions-with-toxic-and-non-toxic-species-from-the-genera-prorocentrum-coolia-and-gambierdiscus
#9
María García-Portela, Pilar Riobó, José Mariano Franco, Rosa Mª Bañuelos, Francisco Rodríguez
The genus Ostreopsis includes several toxic species that can develop blooms in benthic ecosystems, with potential harmful consequences for human health and marine invertebrates. Despite of this, little is known about the allelopathic interactions between these organisms and other co-occurring microalgae that exploit similar spatial and nutrient resources in benthic ecosystems. The aim of this study was to follow these interactions in cultures of two Ostreopsis ribotypes with different toxin profiles (O. cf...
December 2016: Harmful Algae
https://www.readbyqxmd.com/read/28070486/genome-sequencing-and-annotation-of-geobacillus-sp-1017-a-hydrocarbon-oxidizing-thermophilic-bacterium-isolated-from-a-heavy-oil-reservoir-china
#10
Vitaly V Kadnikov, Andrey V Mardanov, Andrey B Poltaraus, Diyana S Sokolova, Ekaterina M Semenova, Nikolay V Ravin, Tatiyana P Tourova, Tamara N Nazina
The draft genome sequence of Geobacillus sp. strain 1017, a thermophilic aerobic oil-oxidizing bacterium isolated from formation water of the Dagang high-temperature oilfield, China, is presented here. The genome comprised 3.6 Mbp, with the G + C content of 51.74%. The strain had a number of genes responsible for numerous metabolic and transport systems, exopolysaccharide biosynthesis, and decomposition of sugars and aromatic compounds, as well as the genes related to resistance to metals and metalloids...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/28064049/anti-diarrhoeal-therapeutic-potential-and-safety-assessment-of-sulphated-polysaccharide-fraction-from-gracilaria-intermedia-seaweed-in-mice
#11
Ana Carolina M Leódido, Luis E C Costa, Thiago S L Araújo, Douglas S Costa, Nayara A Sousa, Luan K M Souza, Francisca B M Sousa, Manoel D S Filho, Daniel F P Vasconcelos, Felipe R P Silva, Kerolayne M Nogueira, Alyne R Araújo, Francisco Clark N Barros, Ana Lúcia P Freitas, Jand Venes R Medeiros
Sulphated polysaccharides extracted from algae have been extensively studied for their diverse biological activities. Thus, the purpose of this study was to evaluate the chemical composition, the anti-diarrhoeal effect and acute toxicity of a sulphated polysaccharide fraction obtained from Gracilaria intermedia (SP-Gi). Initially, the FT-IR of SP-Gi revealed to be an agaran with sulphation at C-6 of the l-galactosyl residues. The anti-diarrhoeal activity of SP-Gi was evaluated in a castor oil-induced diarrhoea model...
January 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28056772/in-silico-screening-for-candidate-chassis-strains-of-free-fatty-acid-producing-cyanobacteria
#12
Olaa Motwalli, Magbubah Essack, Boris R Jankovic, Boyang Ji, Xinyao Liu, Hifzur Rahman Ansari, Robert Hoehndorf, Xin Gao, Stefan T Arold, Katsuhiko Mineta, John A C Archer, Takashi Gojobori, Ivan Mijakovic, Vladimir B Bajic
BACKGROUND: Finding a source from which high-energy-density biofuels can be derived at an industrial scale has become an urgent challenge for renewable energy production. Some microorganisms can produce free fatty acids (FFA) as precursors towards such high-energy-density biofuels. In particular, photosynthetic cyanobacteria are capable of directly converting carbon dioxide into FFA. However, current engineered strains need several rounds of engineering to reach the level of production of FFA to be commercially viable; thus new chassis strains that require less engineering are needed...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28053703/rubeoparvulum-massiliense-gen-nov-sp-nov-a-new-bacterial-genus-isolated-from-the-human-gut-of-a-senegalese-infant-with-severe-acute-malnutrition
#13
M Tidjani Alou, J Rathored, J-C Lagier, S Khelaifia, C Michelle, C Sokhna, A Diallo, A B Diallo, P-E Fournier, D Raoult, S Edouard
Rubeoparvulum massiliense strain mt6(T) was isolated from the gut microbiota of a severely malnourished boy from Senegal and consisted of facultative anaerobic, spore-forming, nonmotile and Gram-negative rods. R. massiliense showed a 92% similarity with the 16S rRNA of Bacillus mannanilyticus. The genome of strain mt6(T) is 2 843 796 bp long with a 43.75% G+C content. It contains 2735 protein-coding genes and 76 RNA genes, among which are nine rRNA genes.
January 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28053587/antimicrobial-antioxidant-properties-and-chemical-composition-of-seaweeds-collected-from-saudi-arabia-red-sea-and-arabian-gulf
#14
Nadine M S Moubayed, Hadeel Jawad Al Houri, Manal M Al Khulaifi, Dunia A Al Farraj
The present study demonstrates the antibacterial activity of selected brown and green marine algae collected from Saudi Arabia Red Sea and Arabian Gulf. The methanolic and acetone extracts were tested against gram positive, gram negative bacteria and Candida albicans in an attempt to be used as an alternative to commonly used antibiotics. Both brown seaweed species Sargassum latifolium B and Sargassum platycarpum A methanolic extracts were found to be active against gram positive than gram negative; however, S...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28050856/improved-endoglucanase-production-and-mycelial-biomass-of-some-ericoid-fungi
#15
O R Adeoyo, B I Pletschke, J F Dames
Fungal species associated with ericaceous plant roots produce a number of enzymes and other bio-active metabolites in order to enhance survival of their host plants in natural environments. This study focussed on endoglucanase production from root associated ericoid mycorrhizal and dark septate endophytic fungal isolates. Out of the five fungal isolates screened, Leohumicola sp. (ChemRU330/PPRI 13195) had the highest relative enzyme activity and was tested along with isolates belonging to Hyloscyphaceae (EdRU083/PPRI 17284) and Leotiomycetes (EdRU002/PPRI 17261) for endoglucanase production under different pH and nutritional conditions that included: carbon sources, nitrogen sources and metal ions, at an optimum temperature of 28 °C...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28050635/biotransformation-and-reduction-of-estrogenicity-of-bisphenol-a-by-the-biphenyl-degrading-cupriavidus-basilensis
#16
Marie-Katherin Zühlke, Rabea Schlüter, Annett Mikolasch, Daniela Zühlke, Martin Giersberg, Henning Schindler, Ann-Kristin Henning, Heidi Frenzel, Elke Hammer, Michael Lalk, Uwe T Bornscheuer, Katharina Riedel, Gotthard Kunze, Frieder Schauer
The biphenyl-degrading Gram-negative bacterium Cupriavidus basilensis (formerly Ralstonia sp.) SBUG 290 uses various aromatic compounds as carbon and energy sources and has a high capacity to transform bisphenol A (BPA), which is a hormonally active substance structurally related to biphenyl. Biphenyl-grown cells initially hydroxylated BPA and converted it to four additional products by using three different transformation pathways: (a) formation of multiple hydroxylated BPA, (b) ring fission, and (c) transamination followed by acetylation or dimerization...
January 3, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28043007/molecular-and-morphological-investigations-of-the-stauros-bearing-raphid-pennate-diatoms-bacillariophyceae-craspedostauros-e-j-cox-and-staurotropis-t-b-b-paddock-and-their-relationship-to-the-rest-of-the-mastogloiales
#17
Matt P Ashworth, Christopher S Lobban, Andrzej Witkowski, Edward C Theriot, Meeshal J Sabir, Mohammad N Baeshen, Nahid H Hajarah, Nabih A Baeshen, Jamal S Sabir, Robert K Jansen
Several lineages of raphe-bearing diatoms possess a "stauros," which is a transverse, usually thickened area free of pores across the center of the valve. It has been suggested that this structure has evolved several times across the raphid diatoms, but we have noticed similarities beyond the stauros between two marine genera-Craspedostauros and Staurotropis-in the structure of their pore occlusions. We have isolated, cultured and extracted DNA from several strains of both genera to infer the phylogenetic relationship between these taxa, as well as test the suggested relationship of Craspedostauros to Achnanthes and Mastogloia based on plastid morphology...
November 17, 2016: Protist
https://www.readbyqxmd.com/read/28036311/etiological-profile-of-early-neonatal-bacterial-sepsis-by-multiplex-qpcr
#18
Walter P Silva-Junior, Almir S Martins, Paula C N Xavier, Kelly L A Appel, Silvio A Oliveira Junior, Durval B Palhares
INTRODUCTION: Given the major impact in terms of morbidity and mortality that episodes of early neonatal sepsis (ENS) have on both newborns and health systems, this study aimed to identify the etiological profile of early neonatal bacterial sepsis by a multiplex quantitative real-time polymerase chain reaction (qPCR). METHODOLOGY: Blood samples from newborns diagnosed with clinical ENS and hospitalized in neonatal intensive care units (NICUs) were collected and analyzed using the multiplex qPCR method to detect Streptococcus agalactiae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter sp...
December 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28032538/alkalicoccus-halolimnae-gen-nov-sp-nov-a-moderately-halophilic-bacterium-isolated-from-a-salt-lake-and-reclassification-of-bacillus-saliphilus-as-alkalicoccus-saliphilus-comb-nov
#19
Baisuo Zhao, Weidong Lu, Shanshan Zhang, Kang Liu, Yanchun Yan, Jun Li
A Gram-stain-positive, cocci-shaped, non-spore-forming, and moderately halophilic bacterium, designed BZ-SZ-XJ29T, was isolated from a salt lake of China. On the basis of 16S rRNA gene sequence similarity, the most closely phylogenetic relatives were Bacillus saliphilus 6AGT (97.3 %) and other five Bacillus species (95.4-96.3 %). However, it shared only 89.5 % with Bacillus subtilis subsp. subtilis DSM 10T, indicating that this isolate might not be a member of Bacillus genus. The genomic DNA G+C content was 40...
December 29, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28032205/enterovirga-rhinocerotis-gen-nov-sp-nov-isolated-from-rhinoceros-unicornis-faeces
#20
Xiu Chen, Qin-Yuan Li, Gui-Ding Li, Hui Lei, Yi Jiang, Li Han, Xue-Shi Huang, Cheng-Lin Jiang
A novel strain, YIM 100770(T), was isolated from Rhinoceros unicornis faeces collected from Yunnan Wild Animal Park, China. The taxonomic status was determined based on the physiological, biochemical and phylogenetic characteristics. Strain YIM 100770(T) was observed to be rod-shaped, non-motile, Gram-stain negative and aerobic. The G+C content of the genomic DNA was determined to be 68.5 mol%. The cells of strain YIM 100770(T) contain ubiquinone Q-10 as the respiratory quinone. The major fatty acids (>1%) were identified as Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 78...
December 28, 2016: Antonie Van Leeuwenhoek
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