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https://www.readbyqxmd.com/read/29235513/metabolic-engineering-of-the-pentose-phosphate-pathway-for-enhanced-limonene-production-in-the-cyanobacterium-synechocysti-s-sp-pcc-6803
#1
Po-Cheng Lin, Rajib Saha, Fuzhong Zhang, Himadri B Pakrasi
Isoprenoids are diverse natural compounds, which have various applications as pharmaceuticals, fragrances, and solvents. The low yield of isoprenoids in plants makes them difficult for cost-effective production, and chemical synthesis of complex isoprenoids is impractical. Microbial production of isoprenoids has been considered as a promising approach to increase the yield. In this study, we engineered the model cyanobacterium Synechocystis sp. PCC 6803 for sustainable production of a commercially valuable isoprenoid, limonene...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29231844/collismycin-c-from-the-micronesian-marine-bacterium-streptomyces-sp-mc025-inhibits-staphylococcus-aureus-biofilm-formation
#2
Jin-Hyung Lee, Eonmi Kim, Hyukjae Choi, Jintae Lee
Biofilm formation plays a critical role in antimicrobial resistance in Staphylococcus aureus. Here, we investigated the potential of crude extracts of 79 Micronesian marine microorganisms to inhibit S. aureus biofilm formation. An extract of Streptomyces sp. MC025 inhibited S. aureus biofilm formation. Bioactivity-guided isolation led to the isolation of a series of 2,2'-bipyridines: collismycin B (1), collismycin C (2), SF2738 D (3), SF2738 F (4), pyrisulfoxin A (5), and pyrisulfoxin B (6). Among these bipyridines, collismycin C (2) was found to be the most effective inhibitor of biofilm formation by methicillin-sensitive S...
December 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29229883/in-vitro-leishmanicidal-antibacterial-and-antitumour-potential-of-anhydrocochlioquinone-a-obtained-from-the-fungus-cochliobolus-sp
#3
Fernanda F Campos, Jonas P Ramos, Djalma M DE Oliveira, Tania M A Alves, Elaine M DE Souza-Fagundes, Carlos L Zani, Fabio C Sampaio, Attilio Converti, Betania B Cota
The bioassay-guided fractionation of the ethyl acetate extract of the fungus Cochliobolus sp. highlighted leishmanicidal activity and allowed for anhydrocochlioquinone A (ANDC-A) isolation. MS, 1D and 2D NMR spectra of this compound were in agreement with those published in the literature. ANDC-A exhibited leishmanicidal activity with EC 50 value of 22.4 microgram/mL (44 mu M) and, when submitted to the microdilution assay against Gram-ositive and Gram-negative bacteria, showed a minimal inhibitory concentration against Staphylococcus aureus ATCC 25295 of 128 microgram/mL (248...
December 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/29225730/draft-genome-sequences-of-bradyrhizobium-shewense-sp-nov-err11t-and-bradyrhizobium-yuanmingense-ccbau-10071t
#4
Aregu Amsalu Aserse, Tanja Woyke, Nikos C Kyrpides, William B Whitman, Kristina Lindström
The type strain of the prospective 10.1601/nm.30737 sp. nov. ERR11T, was isolated from a nodule of the leguminous tree Erythrina brucei native to Ethiopia. The type strain 10.1601/nm.1463 10.1601/strainfinder?urlappend=%3Fid%3DCCBAU+10071 T, was isolated from the nodules of Lespedeza cuneata in Beijing, China. The genomes of ERR11T and 10.1601/strainfinder?urlappend=%3Fid%3DCCBAU+10071 T were sequenced by DOE-JGI and deposited at the DOE-JGI genome portal as well as at the European Nucleotide Archive. The genome of ERR11T is 9,163,226 bp in length and has 102 scaffolds, containing 8548 protein-coding and 86 RNA genes...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/29216280/phenotypic-characterization-of-synechocystis-sp-pcc-6803-substrains-reveals-differences-in-sensitivity-to-abiotic-stress
#5
Tomáš Zavřel, Petra Očenášová, Jan Červený
Synechocystis sp. PCC 6803 is a widely used model cyanobacterium, whose substrains can vary on both genotype and phenotype levels. Previously described phenotypic variations include ability of mixotrophic growth, ability of movement on agar plates and variations in pigments composition or cell size. In this study, we report for the first time significant variation among Synechocystis substrains in complex cellular traits such as growth rate, photosynthesis efficiency, cellular dry weight and cellular composition (including protein or carbohydrates content)...
2017: PloS One
https://www.readbyqxmd.com/read/29215956/effect-of-thrombopoietin-receptor-agonists-on-markers-of-coagulation-and-p-selectin-in-patients-with-immune-thrombocytopenia
#6
Lamya Garabet, Waleed Ghanima, Christine Monceyron Jonassen, Vibe Skov, René Holst, Marie-Christine Mowinckel, C Hasselbalc Hans, A Kruse Torben, Mads Thomassen, Howard Liebman, James B Bussel, Per Morten Sandset
Thrombopoietin-receptor-agonists (TPO-RA) are effective treatments of immune thrombocytopenia (ITP). Previous long-term TPO-RA clinical trials have shown that thrombotic events occurred in 6% of TPO-RA-treated ITP patients. To explore the increased risk of thrombosis, the effects of TPO-RA on markers of coagulation and P-selectin were studied. The study comprised two ITP cohorts and controls. Cohort 1 included 26 patients with sequential samples acquired before and during treatment with TPO-RA. Cohort 2 included a single sample in 18 patients on TPO-RA for more than one year...
December 7, 2017: Platelets
https://www.readbyqxmd.com/read/29215662/-validation-of-the-scale-of-hope-in-terminal-illness-for-relatives-brief-version-shti-b-validity-and-reliability-analysis
#7
M Villacieros Durban, J C Bermejo, H Hassoun
BACKGROUND: Bermejo and Villacieros' Scale of Hope in Terminal Disease (SHTD) specifically collects meanings of hope facing terminal disease, including considerations relating to psycho-emotional support and that have a transcendental sense. The objective of this paper is to validate the SHTD abbreviated and rephrased to adapt all the items to a single domain. METHODS: Starting from the published SHTD, an exploratory factor analysis (EFA) was carried out with a sample of 177 valid questionnaires...
December 7, 2017: Anales del Sistema Sanitario de Navarra
https://www.readbyqxmd.com/read/29209908/purification-and-characterization-of-exo-inulinase-from-paenibacillus-sp-d9-strain
#8
S Jeza, S B Maseko, J Lin
This study intended to purify and characterise exo-inulinase of diesel-degrading Paenibacillus sp. D9. The whole genome sequencing of Paenibacillus sp. D9 revealed to possess the sacC gene that is encoded as exo-inulinase/levanase. This isolate was capable of producing a maximum of 50.9 IU/mL of exo-inulinase activity within 3 days at 30 °C, 200 rpm and pH of 7.0 on minimal salt medium agar supplemented with 1% (w/v) inulin. An exo-inulinase of 58.5 kDa was purified using ammonium sulphate precipitation, HiTrap QFF column and MMC column chromatographies with a specific activity of 4333 IU/mg, 7...
December 5, 2017: Protein Journal
https://www.readbyqxmd.com/read/29205133/pontibacter-brevis-sp-nov-isolated-from-rhizosphere-soil-of-tamarix-ramosissima
#9
Ghenijan Osman, Yan Gao, Ning Wang, Otkur Mahmud, Jian Sun, Tao Zhang, Faqiang Zhan, Zhidong Zhang, Kai Lou
A Gram-stain-negative, oval-shaped and light pink pigmented bacterium, designated XAAS-2T, was isolated from rhizosphere soil of Tamarix ramosissima. The sole respiratory quinone of the type strain XAAS-2T was MK-7, and the principal cellular fatty acids were summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C15 : 0. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. 16S rRNA gene sequence analysis indicated that strain XAAS-2T belonged to the genus Pontibacter within the family Cytophagaceae with sequence similarities of 93...
December 5, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29202868/sp-a2-contributes-to-mirna-mediated-sex-differences-in-response-to-oxidative-stress-pro-inflammatory-anti-apoptotic-and-anti-oxidant-pathways-are-involved
#10
George T Noutsios, Nithyananda Thorenoor, Xuesheng Zhang, David S Phelps, Todd M Umstead, Faryal Durrani, Joanna Floros
BACKGROUND: Human innate host defense molecules, surfactant protein A1 (SP-A1), and SP-A2 differentially affect the function and proteome of the alveolar macrophage (AM). We hypothesized that SP-A genes differentially regulate the AM miRNome. METHODS: Humanized transgenic mice expressing SP-A1 and SP-A2 were subjected to O3-induced oxidative stress (OxS) or filtered air (FA), AMs were isolated, and miRNA levels were measured. RESULTS: In SP-A2 males, we found significant changes in miRNome in terms of sex and sex-OxS effects, with 11 miRNAs differentially expressed under OxS...
December 4, 2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/29199333/-study-on-the-sealing-ability-of-biological-root-canal-sealer-iroot-sp-in-root-canal-filling
#11
Ning Zhang, Xin Li, Lei-Ying Miao, Juan Wu, Chao Liu, Wei-Dong Yang
PURPOSE: To investigate the sealing ability of biological root canal sealer iRoot SP in over-root canal filling. METHODS:After completion of root canal preparation, 48 extracted human single-canal teeth were used. The ratio of mesio-distal root canal diameter to bucco-lingual diameter was less than 0.5. The teeth were randomly divided into group A, B and C. The root canals were obturated with root canal filling agent (group A), AH Plus (group B) and iRoot SP (group C), respectively; the apical leakage was evaluated by dye penetration method and transparent teeth technique...
August 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29192600/magazines-in-waiting-areas-of-hospital-a-forgotten-microbial-reservoir
#12
Mathias Adé, Sandrine Burger, Anaelle Cuntzmann, Julien Exinger, Olivier Meunier
The hospital environment is a potential source of microbial contamination. Thus, the magazines in hospital's waiting rooms are handled by patients and visitors whose health and hygiene conditions can vary widely. In this context, we had measured the microbial load on the surface of magazines. Fifteen magazines from 5 waiting rooms of hospital are sampled by agar prints at the areas taken in hand. The agar plates are incubated at 30̊C for 72h. The colonies are counted and identified by MALDI-TOF mass spectrometry (Vitek®-MS)...
December 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29189900/hygrocin-c-from-marine-derived-streptomyces-sp-scsgaa-0027-inhibits-biofilm-formation-in-bacillus-amyloliquefaciens-scsgab0082-isolated-from-south-china-sea-gorgonian
#13
Jie Wang, Xu-Hua Nong, Muhammad Amin, Shu-Hua Qi
Several ansamycins have been reported to inhibit bacterial biofilm formation and accelerate the eradication of developed biofilms, but little is known about the effect of hygrocin C, an ansamycin, on bacterial biofilm formation. Here, hygrocin C was isolated from the marine-derived Streptomyces sp. SCSGAA 0027 and reported for the first time to be capable of inhibiting the biofilm formation of Staphylococcus aureus and Bacillus amyloliquefaciens SCSGAB0082 with the production of anti-microbial lipopeptides from South China Sea gorgonian Subergorgia suberosa at concentrations of less than minimum inhibitory concentrations...
November 30, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29186895/effects-of-adding-clostridium-sp-wj06-on-intestinal-morphology-and-microbial-diversity-of-growing-pigs-fed-with-natural-deoxynivalenol-contaminated-wheat
#14
FuChang Li, JinQuan Wang, LiBo Huang, HongJu Chen, ChunYang Wang
Deoxynivalenol (DON) is commonly detected in cereals, and is a threat to human and animal health. The effects of microbiological detoxification are now being widely studied. A total of 24 pigs (over four months) were randomly divided into three treatments. Treatment A was fed with a basal diet as the control group. Treatment B was fed with naturally DON-contaminated wheat as a negative control group. Treatment C was fed with a contaminated diet that also had Clostridium sp. WJ06, which was used as a detoxicant...
November 27, 2017: Toxins
https://www.readbyqxmd.com/read/29185938/thauera-propionica-sp-nov-isolated-from-downstream-sediment-sample-of-the-river-ganges-kanpur-india
#15
Deepika Pal, Ayanka Bhardwaj, Sarabjeet Kour Sudan, Navjot Kaur, Munesh Kumari, Bhawana Bisht, Bhawna Vyas, Srinivasan Krishnamurthi, Shanmugam Mayilraj
A Gram-stain-negative, non-endospore-producing, short-rod strain, KNDSS-Mac4T, was isolated from a downstream sediment sample of the river Ganges, Kanpur, India and studied by using the polyphasic taxonomic approach. 16S rRNA gene sequence analysis uncovered that the strain had similarity to species of the genus Thauera and formed a distinct phylogenetic cluster with Thauera humireducens KACC16524T. However, KNDSS-Mac4T showed closest phylogenetic affiliation to Thauera aminoaromatica DSM 14742T with 16S rRNA gene sequence similarity of 98...
November 29, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29184110/differential-effects-on-membrane-permeability-and-viability-of-human-keratinocyte-cells-undergoing-very-low-intensity-megasonic-fields
#16
F Domenici, F Brasili, S Giantulli, B Cerroni, A Bedini, C Giliberti, R Palomba, I Silvestri, S Morrone, G Paradossi, M Mattei, F Bordi
Among different therapeutic applications of Ultrasound (US), transient membrane sonoporation (SP) - a temporary, non-lethal porosity, mechanically induced in cell membranes through US exposure - represents a compelling opportunity towards an efficient and safe drug delivery. Nevertheless, progresses in this field have been limited by an insufficient understanding of the potential cytotoxic effects of US related to the failure of the cellular repair and to the possible activation of inflammatory pathway. In this framework we studied the in vitro effects of very low-intensity US on a human keratinocyte cell line, which represents an ideal model system of skin protective barrier cells which are the first to be involved during medical US treatments...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29182145/experimental-zika-virus-infection-of-neotropical-primates
#17
John A Vanchiere, Julio C Ruiz, Alan G Brady, Thomas J Kuehl, Lawrence E Williams, Wallace B Baze, Gregory K Wilkerson, Pramod N Nehete, Gloria B McClure, Donna L Rogers, Shannan L Rossi, Sasha R Azar, Christopher M Roundy, Scott C Weaver, Nikos Vasilakis, Joe H Simmons, Christian R Abee
The establishment of a sylvatic reservoir of Zika virus (ZIKV) in the Americas is dependent on the susceptibility of primates of sufficient population density, the duration and magnitude of viremia, and their exposure to the human mosquito-borne transmission cycle. To assess the susceptibility of squirrel (Saimiri sp.) and owl monkeys (Aotus sp.) to infection, we inoculated four animals of each species with ZIKV from the current epidemic. Viremia in the absence of detectible disease was observed in both species and seroconversion occurred by day 28...
November 27, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29178845/classification-of-a-new-phytoplasmas-subgroup-16srii-w-associated-with-crotalaria-witches-broom-diseases-in-oman-based-on-multigene-sequence-analysis
#18
Ali Al-Subhi, Saskia A Hogenhout, Rashid A Al-Yahyai, Abdullah M Al-Sadi
BACKGROUND: Crotalaria aegyptiaca, a low shrub is commonly observed in the sandy soils of wadis desert and is found throughout all regions in Oman. A survey for phytoplasma diseases was conducted. During a survey in a wild area in the northern regions of Oman in 2015, typical symptoms of phytoplasma infection were observed on C. aegyptiaca plants. The infected plants showed an excessive proliferation of their shoots and small leaves. RESULTS: The presence of phytoplasma in the phloem tissue of symptomatic C...
November 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29176770/high-trypanosoma-spp-diversity-is-maintained-by-bats-and-triatomines-in-esp%C3%A3-rito-santo-state-brazil
#19
Maria Augusta Dario, Cristiane Varella Lisboa, Luciana M Costa, Ricardo Moratelli, Monique Pereira Nascimento, Leonora Pires Costa, Yuri Luiz Reis Leite, Martin S Llewellyn, Samanta Cristina das Chagas Xavier, André Luiz Rodrigues Roque, Ana Maria Jansen
The aim of this study was to reevaluate the ecology of an area in the Atlantic Forest, southeast Brazil, where Chagas disease (CD) has been found to occur. In a previous study, immediately after the occurrence of a CD case, we did not observe any sylvatic small mammals or dogs with Trypanosoma cruzi cruzi infections, but Triatoma vitticeps presented high T. c. cruzi infection rates. In this study, we investigated bats together with non-volant mammals, dogs, and triatomines to explore other possible T. c. cruzi reservoirs/hosts in the area...
2017: PloS One
https://www.readbyqxmd.com/read/29168149/revision-of-podocotyloides-yamaguti-1934-digenea-opecoelidae-resurrection-of-pedunculacetabulum-yamaguti-1934-and-the-naming-of-a-cryptic-opecoelid-species
#20
Storm B Martin, Scott C Cutmore, Thomas H Cribb
Despite morphological and ecological inconsistencies among species, all plagioporine opecoelids with a pedunculate ventral sucker are currently considered to belong in the genus Podocotyloides Yamaguti, 1934. We revise the genus based on combined morphological and phylogenetic analyses of novel material collected from haemulid fishes in Queensland waters that we interpret to represent species congeneric with the type-species, Pod. petalophallus Yamaguti, 1934, also known from a haemulid, off Japan. Our phylogenetic analysis demonstrates polyphyly of Podocotyloides; prompts us to resurrect Pedunculacetabulum Yamaguti, 1934; and suggests that Pod...
November 22, 2017: Systematic Parasitology
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