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https://www.readbyqxmd.com/read/27927208/use-of-a-sec-signal-peptide-library-from-bacillus-subtilis-for-the-optimization-of-cutinase-secretion-in-corynebacterium-glutamicum
#1
Johannes Hemmerich, Peter Rohe, Britta Kleine, Sarah Jurischka, Wolfgang Wiechert, Roland Freudl, Marco Oldiges
BACKGROUND: Technical bulk enzymes represent a huge market, and the extracellular production of such enzymes is favorable due to lowered cost for product recovery. Protein secretion can be achieved via general secretion (Sec) pathway. Specific sequences, signal peptides (SPs), are necessary to direct the target protein into the translocation machinery. For example, >150 Sec-specific SPs have been identified for Bacillus subtilis alone. As the best SP for a target protein of choice cannot be predicted a priori, screening of homologous SPs has been shown to be a powerful tool for different expression organisms...
December 7, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27921147/can-the-alendronate-dosage-be-altered-when-combined-with-high-frequency-loading-in-osteoporosis-treatment
#2
C B Correa, G V Camargos, M Chatterjee, M F Mesquita, A A Del Bel Cury, I Naert, J Duyck, K Vandamme
: Alendronate therapy has been associated with serious side effects. Altering the alendronate concentration and combining with high-frequency loading as mechanical intervention was explored in this animal study as a treatment for osteoporosis. The bone anabolic potency of high-frequency loading was overruled by the different alendronate dosages applied in the present study. Further exploration of reduced hormonal therapy associated with mechanical interventions in osteoporosis treatment should be sought...
December 5, 2016: Osteoporosis International
https://www.readbyqxmd.com/read/27917838/overcoming-a-hemihedral-twinning-problem-in-tetrahydrofolate-dependent-o-demethylase-crystals-by-the-microseeding-method
#3
Ayaka Harada, Yukari Sato, Naofumi Kamimura, Nagarajan Venugopalan, Eiji Masai, Toshiya Senda
A tetrahydrofolate-dependent O-demethylase, LigM, from Sphingobium sp. SYK-6 was crystallized by the hanging-drop vapour-diffusion method. However, the obtained P3121 or P3221 crystals, which diffracted to 2.5-3.3 Å resolution, were hemihedrally twinned. To overcome the twinning problem, microseeding using P3121/P3221 crystals as microseeds was performed with optimization of the reservoir conditions. As a result, another crystal form was obtained. The newly obtained crystal diffracted to 2.5-3.0 Å resolution and belonged to space group P21212, with unit-cell parameters a = 102...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27917692/once-daily-high-dose-tigecycline-pharmacokinetic-pharmacodynamic-based-dosing-for-optimal-clinical-effectiveness-dosing-matters-revisited
#4
Burke A Cunha, Jeffrey Baron, Cheston B Cunha
Tigecycline has emerged as first line therapy for serious systemic infections due to important pathogens (except P. aeruginosa and Proteus sp.), including multi-drug resistant (MDR) and Gram negative bacilli (GNB), including carbapenem resistant Enterobacteriae. Tigecycline has a "low resistance potential," is protective against C. difficile, and is often the only antibiotic effective against MDR GNB, e.g., Klebsiella sp. Areas covered: Standard dose tigecycline therapy has been used for intra-abdominal infections, complicated skin/skin structure infections (cSSSIs), and CAP...
December 3, 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27909705/transition-metal-catalyzed-iodine-iii-mediated-nitrene-transfer-reactions-efficient-tools-for-challenging-syntheses
#5
B Darses, R Rodrigues, L Neuville, M Mazurais, P Dauban
The significant progress made in the area of transition metal-catalyzed nitrene transfer into C-H or C[double bond, length as m-dash]C bonds mediated by iodine(iii) oxidants has been translated into many elegant synthetic applications. This feature article summarizes the main total syntheses that take advantage of such reactions, thereby highlighting that catalytic C(sp(3))-H amination and alkene aziridination reactions have carved out their place in the organic chemist's toolbox.
December 2, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27909130/the-use-of-ultra-low-dose-ct-scans-for-the-evaluation-of-limb-fractures-is-the-reduced-effective-dose-using-ct-in-orthopaedic-injury-reduction-protocol-effective
#6
S R Konda, A M Goch, P Leucht, A Christiano, S Gyftopoulos, G Yoeli, K A Egol
AIMS: To evaluate whether an ultra-low-dose CT protocol can diagnose selected limb fractures as well as conventional CT (C-CT). PATIENTS AND METHODS: We prospectively studied 40 consecutive patients with a limb fracture in whom a CT scan was indicated. These were scanned using an ultra-low-dose CT Reduced Effective Dose Using Computed Tomography In Orthopaedic Injury (REDUCTION) protocol. Studies from 16 selected cases were compared with 16 C-CT scans matched for age, gender and type of fracture...
December 2016: Bone & Joint Journal
https://www.readbyqxmd.com/read/27902348/micropepsis-pineolensis-gen-nov-sp-nov-a-mildly-acidophilic-alphaproteobacterium-isolated-from-a-poor-fen-and-proposal-of-micropepsaceae-fam-nov-within-micropepsales-ord-nov
#7
Austin B Harbison, Laiken E Price, Michael D Flythe, Suzanna L Brauer
An obligately anaerobic, mildly acidophilic, fermentative alphaproteobacterium, designated strain CS4 T, was isolated from an acidic, oligotrophic (nutrient-poor) poor fen located near Pineola, NC, USA. Cultures contained Gram-negative staining, slightly curved, non-motile, non-spore forming, non-prosthecate rods (0.1-0.4 µm in diameter and 0.4-4 µm long, depending, in part, on the growth substrate). Growth optima were 35° C (range 15 - 35°C), pH 5.6 (range of 5.0 - 6.8), and with 0-50 mM added NaCl (range 0-100 mM added NaCl)...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902346/hymenobacter-rutilus-sp-nov-isolated-from-marine-sediment-in-arctic
#8
Myong Chol Kim, Chol Myong Kim, Ok Chol Kang, Yumin Zhang, Zuobing Liu, Danzeng Wangmu, Ziyan Wei, Yao Huang, Fang Peng
Strain K2-33028T, appeared brick-red colony on R2A plate, was isolated from marine sediment sample in Kings Bay, Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2-33028T belonged to the genus Hymenobacter. Cells were Gram-reaction-negative, non-spore-forming, aerobic, rod-shaped bacterium without motility. Growth occurred at 4-37℃ (optimum 28℃) and at pH 6.0-8.0 (optimum pH 7.0). Cells contained menaquinone-7, as the main respiratory quinone, and iso-C15:0, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:1 ω5c, summed feature 4 (comprising anteiso-C17:1 B and/or iso-C17:1 I) and anteiso-C15:0 as the major cellular fatty acids...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902326/caenimicrobium-hargitense-gen-nov-sp-nov-a-new-member-of-the-family-alcaligenaceae-betaproteobacteria-isolated-from-activated-sludge
#9
Tamas Felfoldi, Peter Schumann, Anikó Mentes, Zsuzsa Kéki, István Máthé, Erika Tóth
A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared the highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7%), various Bordetella species (97.3-97.0%) and Candidimonas nitroreducens LMG 24812T (97.0%). Strain CGII-59m2T was rod-shaped, non-motile, oxidase and catalase positive. The predominant fatty acids were C16:1ω7c, C16:0, cycloC17:0 and C18:1ω7c, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid...
November 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902318/streptomyces-capparidis-sp-nov-a-novel-endophytic-actinobacterium-isolated-from-fruits-of-capparis-spinosa-l
#10
Hong-Fei Wang, Qiu-Li Li, Min Xiao, Yong-Guang Zhang, Xing-Kui Zhou, Manik Prabhu Narsing Rao, Yan-Qing Duan, Wen-Jun Li
A novel endophytic actinobactrium strain, designated EGI 6500195T, was isolated from Capparis spinosa. Growth occurred at 10-45 °C (optimum 30 °C), pH 6-8 (optimum pH 7) and in the presence of 0-1% NaCl (w/v). The strain EGI 6500195T shared the highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686T and less than 97% sequence similarities with other members of the genus Streptomyces. The diagnostic amino acid in the peptidoglycan was LL-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose,fructose and mannose...
October 18, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902271/methylobacillus-methanolivorans-sp-nov-a-novel-non-pigmented-obligately-methylotrophic-bacterium
#11
Elena N Kaparullina, Yuri A Trotsenko, Nina V Doronina
Three strains of obligately methylotrophic Betaproteobacteria (ZT, SP and M3) with the ribulose monophosphate pathway of C1 assimilation are described. The isolates are strictly aerobic, Gram-stain-negative, asporogenous, motile (strains ZT and M3) or non-motile (strain SP) rods multiplying by binary fisson, mesophilic and neutrophilic. All three strains utilize methanol but only strains SP and M3 utilize methylamine as the carbon and energy sources. The prevailing cellular fatty acids are straight-chain saturated C16 : 0 and unsaturated C16 : 1ω7c acids...
November 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902266/rhodococcus-sovatensis-sp-nov-a-novel-actinomycete-isolated-from-the-hypersaline-and-heliothermal-lake-ursu-sovata-romania
#12
András Táncsics, István Máthé, Tibor Benedek, Erika M Tóth, Ewelina Atasayar, Cathrin Spröer, Károly Márialigeti, Tamás Felföldi, Balázs Kriszt
A Gram-positive, strictly aerobic, mesophilic bacterium, designated H004T, was isolated from water sample of the hypersaline and heliothermal Lake Ursu, Sovata, Romania. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain H004T formed a distinct phyletic lineage within the genus Rhodococcus. It shared the highest 16S rRNA gene similarity with Rhodococcus yunnanensis YIM 70056T (98.80%), followed by Rhodococcus fascians LMG 3623T (98.73%), Rhodococcus cercidiphylli YIM 65003T (98.73%), Rhodococcus cerastii C5T (98...
October 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902264/hymenobacter-seoulensis-sp-nov-isolated-from-river-water
#13
Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Seung-Yeol Lee, Sangkyu Park, Myung Kyum Kim, Leonid N Ten, Hee-Young Jung
A Gram-stain-negative, aerobic, rod-shaped, nonmotile and pink pigmented bacterial strain, designated 16F7GT, was isolated from river water. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16F7GT belongs to the genus Hymenobacter. The 16S rRNA gene sequence similarity to members of the genus Hymenobacter ranged from 90.5 to 97.4%, and the most closely related strains were Hymenobacter rigui WPCB131T (97.4%) and Hymenobacter xinjiangensis X2-1gT (97.3%). Strain 16F7GT had <70% DNA-DNA relatedness with H...
November 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902263/terrimonas-rhizosphaerae-sp-nov-isolated-from-ginseng-rhizosphere-soil
#14
Song-Ih Han, Ye-Rim Lee, Ju-Ok Kim, Kyung-Sook Whang
The novel isolate belonging to the genus Terrimonas, designated CR94T, was isolated from rhizosphere soil of ginseng field in Geumsan in Korea. Cells of Strain CR94T were strictly aerobic, Gram staining negative, non-motile, non-filamentous single rods. Growth was observed at 10-37℃ (optimum 28℃) and at pH4.0-10.0 (optimum pH6.0). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CR94T belonged to the genus Terrimonas, showing the highest sequence similarity to Terrimonas lutea DYT (97...
November 2, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902209/fimbriiglobus-ruber-gen-nov-sp-nov-a-gemmata-like-planctomycete-from-sphagnum-peat-bog-and-the-proposal-of-gemmataceae-fam-nov
#15
Irina S Kulichevskaya, Anastasia A Ivanova, Olga I Baulina, W Irene C Rijpstra, Jaap S Sinninghe Damsté, Svetlana N Dedysh
An aerobic, budding, dark pink to red-pigmented bacterium was isolated from an acidic boreal Sphagnum peat bog and designated strain SP5T. Cells of this strain were non-motile spheres that were uniformly covered with crateriform pits and fimbria, and tended to form aggregates during growth in liquid media. Strain SP5T was capable of growth at pH values between 4.0 and 6.8 (optimum at pH 5.5-6.0) and at temperatures between 10 and 30oC (optimum 20-25oC). The preferred growth substrates were sugars and some heteropolysaccharides...
October 24, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27898907/apparent-total-tract-macronutrient-digestibility-fecal-characteristics-and-fecal-fermentative-end-product-concentrations-of-healthy-adult-dogs-fed-bioprocessed-soy-protein
#16
A N Beloshapka, M R C de Godoy, K B Detweiler, M Newcomb, K H Ellegård, G C Fahey, K S Swanson
Animal proteins are commonly used in extruded dog foods. Plant-based proteins have a more consistent nutrient profile than animal sources but may contain antinutritional factors, including trypsin inhibitors and oligosaccharides. Bioprocessed soy protein (SP; HP-300; Hamlet Protein, Inc., Findlay, OH) is a processed soy-based product with low antinutritional factor concentrations and high protein quality. The objective was to evaluate the effects of SP on apparent total tract macronutrient digestibility, fecal characteristics, and fecal fermentative end products...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898739/environmental-free-living-amoebae-isolated-from-soil-in-khon-kaen-thailand-antagonize-burkholderia-pseudomallei
#17
Parumon Noinarin, Pisit Chareonsudjai, Pinich Wangsomnuk, Surasak Wongratanacheewin, Sorujsiri Chareonsudjai
Presence of Burkholderia pseudomallei in soil and water is correlated with endemicity of melioidosis in Southeast Asia and northern Australia. Several biological and physico-chemical factors have been shown to influence persistence of B. pseudomallei in the environment of endemic areas. This study was the first to evaluate the interaction of B. pseudomallei with soil amoebae isolated from B. pseudomallei-positive soil site in Khon Kaen, Thailand. Four species of amoebae, Paravahlkampfia ustiana, Acanthamoeba sp...
2016: PloS One
https://www.readbyqxmd.com/read/27897421/nickel-catalyzed-alkoxy-alkyl-interconversion-with-alkylborane-reagents-through-c-o-bond-activation-of-aryl-and-enol-ethers
#18
Lin Guo, Xiangqian Liu, Christoph Baumann, Magnus Rueping
A nickel-catalyzed alkylation of polycyclic aromatic methyl ethers as well as methyl enol ethers with B-alkyl 9-BBN and trialkylborane reagents that involves the cleavage of stable C(sp(2) )-OMe bonds is described. The transformation has a wide substrate scope and good chemoselectivity profile while proceeding under mild reaction conditions; it provides a versatile way to form C(sp(2) )-C(sp(3) ) bonds that does not suffer from β-hydride elimination. Furthermore, a selective and sequential alkylation process by cleavage of inert C-O bonds is presented to demonstrate the advantage of this method...
December 5, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27889889/biosurfactant-production-by-the-crude-oil-degrading-stenotrophomonas-sp-b-2-chemical-characterization-biological-activities-and-environmental-applications
#19
Boutheina Gargouri, María Del Mar Contreras, Sonda Ammar, Antonio Segura-Carretero, Mohamed Bouaziz
In this work, biosurfactant-producing microorganisms were isolated from hydrocarbon-contaminated water collected from Tunisian oilfield. After enrichment and isolation, different bacterial strains were preliminary studied for their biosurfactant/bioemulsifier properties when using crude oil as the unique carbon source. In particular, the isolate strain B-2, a Gram-negative, rod-shaped bacterium, efficiently emulsified crude oil. The extracellular biosurfactant product from this strain presented an emulsification activity above 70% and a hydrophobicity of 71%...
November 26, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27889169/characterization-of-biocide-tolerant-bacteria-isolated-from-cheese-and-dairy-small-medium-enterprises
#20
Ma Luisa Fernández Márquez, Ma José Grande Burgos, Ma Carmen López Aguayo, Rubén Pérez Pulido, Antonio Gálvez, Rosario Lucas
A collection of 120 bacterial isolates from small medium enterprises involved in the production of cow milk and the manufacture of goat cheese were screened for sensitivity to biocides benzalkonium chloride (BC), cetrimide (CT), hexadecylpyridinium chloride (HDP), triclosan (TC), hexachlorophene (CF) and poly-(hexamethylen guanidinium) hydrochloride (PHMG). Nineteen isolates were selected according to biocide tolerance and identified by 16S rDNA sequencing as Lactococcus sp. (6) Enterococcus sp. (1), Lactobacillus sp...
April 2017: Food Microbiology
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