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Andreas Holzinger, Andreas Albert, Siegfried Aigner, Jenny Uhl, Philippe Schmitt-Kopplin, Kateřina Trumhová, Martina Pichrtová
Species of Zygnema form macroscopically visible mats in polar and temperate terrestrial habitats, where they are exposed to environmental stresses. Three previously characterized isolates (Arctic Zygnema sp. B, Antarctic Zygnema sp. C, and temperate Zygnema sp. S) were tested for their tolerance to experimental UV radiation. Samples of young vegetative cells (1 month old) and pre-akinetes (6 months old) were exposed to photosynthetically active radiation (PAR, 400-700 nm, 400 μmol photons m-2  s-1 ) in combination with experimental UV-A (315-400 nm, 5...
February 22, 2018: Protoplasma
Young-Ok Kim, In-Suk Park, Sooyeon Park, Bo-Hye Nam, Dong-Gyun Kim, Seok-Gwan Choi, Jin-Woo Bae, Jung-Hoon Yoon
A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterial strain, designated RA3-3-1T , was isolated from splendid alfonsino (Beryxsplendens) collected from the North Pacific Ocean. Strain RA3-3-1T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA3-3-1T belonged to the genus Bizionia, clustering with the type strain of Bizionia fulviae. Strain RA3-3-1T exhibited 16S rRNA gene sequence similarities of 98...
February 22, 2018: International Journal of Systematic and Evolutionary Microbiology
Yi Sun, Rogie Royce Carandang, Yuta Harada, Shigeru Okada, Kazutoshi Yoshitake, Shuichi Asakawa, Yuichi Nogi, Shigeki Matsunaga, Kentaro Takada
Three new polyketides, lactomycins A (1)-C (3), were isolated from the culture broth of a marine-derived Streptomyces sp. ACT232 as cathepsin B inhibitors. Their structures were determined by a combination of NMR and MS data analyses to be the dephosphorylated derivatives of a phoslactomycin class of metabolites. Lactomycins exhibited cathepsin B inhibitory activity (IC50 0.8 to 4.5 μg/mL). Even though the biosynthetic gene clusters found in the genome of the current strain have high similarity to those of phoslactomycin, neither phoslactomycins nor leustroducsins were detected by LC-MS analyses of the crude extract...
February 21, 2018: Marine Drugs
Svetlana E Belova, Natalia E Suzina, W Irene C Rijpstra, Jaap S Sinninghe Damsté, Svetlana N Dedysh
An isolate of aerobic, Gram-stain-negative, rod-shaped, non-motile and light-pink pigmented bacteria, designated SBC68T , was obtained from slightly decomposed thalli of the lichen Cladonia sp. collected from the forested tundra of north-western Siberia. Cells of this isolate occurred singly, in pairs or in rosettes. These bacteria were acidophilic (optimum growth at pH 4.3-5.6) and mesophilic (optimum growth at 20-30 °C) but were also capable of growth at low temperatures, down to 7 °C. The preferred growth substrates were sugars, some organic acids and lichenan...
February 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Keun Chul Lee, Min Kuk Suh, Mi Kyung Eom, Kwang Kyu Kim, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Yong Kook Shin, Jung-Sook Lee
A novel actinobacterial strain, designated N237T , was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea. Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar. Strain N237T contained iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan. It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids...
February 21, 2018: International Journal of Systematic and Evolutionary Microbiology
E N Frolov, K S Zayulina, D S Kopitsyn, I V Kublanov, E A Bonch-Osmolovskaya, N A Chernyh
An anaerobic sulfate-reducing micro-organism, strain 3408-1T , was isolated from a terrestrial hot spring in Kamchatka peninsula (Russia). The cells were spore-forming rods with a Gram-positive type of cell wall. The new isolate was a moderately thermoacidophilic anaerobe able to grow either by sulfate or thiosulfate respiration with H2 or formate as substrates, or by fermenting yeast extract, maltose, sucrose, glucose and pyruvate. The fermentation products were acetate, CO2 and H2. The pH range for growth was 2...
January 25, 2018: International Journal of Systematic and Evolutionary Microbiology
Dong-Uk Kim, Kwan Woo Kim, Myung-Suk Kang, Ju-Young Kim, Jun Hwee Jang, Myung Kyum Kim
Strain 17mud1-7T , a pink, aerobic, catalase-positive, oxidase-positive and Gram-reaction-negative bacterium, was isolated from wet mud. The isolate grew aerobically at 18-37 °C (optimum 28 °C), at pH 6.0-8.0 (optimum pH 7.0) and in the presence of 0-1 % (w/v) NaCl (optimum 0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 17mud1-7T belonged to the genus Adhaeribacter with highest sequence similarity of 96.4 % to Adhaeribacter aerophilus 6424S-25T . The strain showed the typical chemotaxonomic characteristics of the genus Adhaeribacter, with the presence of menaquinone MK-7 as the respiratory quinone, and summed feature 4 (composed of iso-C17 : 1 I/anteiso-C17 : 1 B), iso-C15 : 0 and C16 : 1ω5c as the major fatty acids are...
February 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Peter Kämpfer, P D Rekha, Hans-Jürgen Busse, A B Arun, P Priyanka, Stefanie P Glaeser
A Gram-stain-negative, aerobic, non-endospore-forming organism, isolated from the rhizosphere sand of a coastal sand dune plant was studied for its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain YU-PRIM-29T was grouped within the genus Halomonas and was most closely related to Halomonas johnsoniae (97.5 %). The 16S rRNA gene sequence similarity to other Halomonas species was <97.5 %. Strain YU-PRIM-29T grew optimally at 28 °C (growth range, 10-36 °C), at a pH of 7-9 (growth range, pH 5...
February 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Na-Ri Shin, Woorim Kang, Euon Jung Tak, Dong-Wook Hyun, Pil Soo Kim, Hyun Sik Kim, June-Young Lee, Hojun Sung, Tae Woong Whon, Jin-Woo Bae
A strictly anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KB1T , was isolated from a faecal sample of a patient with diverticulitis in South Korea. Degeneracies in the 16S rRNA gene sequence of strain KB1T were resolved by cloning, which yielded five different sequences with heterogeneity. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain KB1T formed a monophyletic branch with species in the genus Blautia, with highest sequence similarity to the type strain of Blautia producta (97...
February 9, 2018: International Journal of Systematic and Evolutionary Microbiology
Nattakorn Kuncharoen, Pattama Pittayakhajonwut, Somboon Tanasupawat
A novel endophytic actinomycete, strain WPS1-2T , isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach. Strain WPS1-2T exhibited identical characteristics to the members of the genus Micromonospora. Single spores were observed directly on substrate mycelia. The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose. The predominant menaquinones were MK-10(H8) and MK-10(H10)...
February 9, 2018: International Journal of Systematic and Evolutionary Microbiology
Praveen Rahi, Rashmi Kurli, Aabeejjeet N Pansare, Mitesh Khairnar, Shubhangi Jagtap, Nisha B Patel, Syed G Dastager, Paul A Lawson, Yogesh S Shouche
A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63T , exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234T and Microbacterium kitamiense Kitami C2T ...
February 9, 2018: International Journal of Systematic and Evolutionary Microbiology
József Kukolya, Ildikó Bata-Vidács, Szabina Luzics, Erika Tóth, Zsuzsa Kéki, Peter Schumann, András Táncsics, István Nagy, Ferenc Olasz, Ákos Tóth
A novel Gram-stain-positive bacterial strain, designated as K13T , was isolated from compost and characterized using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, the strain showed highest similarity (93.8 %) to Paenibacillus nanensis MX2-3T . Cells of strain K13T were aerobic, motile rods. The major fatty acids were anteiso C15 : 0 (34.4 %), iso C16 : 0 (17.3 %) and C16 : 0 (10.0 %). The major menaquinone was MK-7, the polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and an aminophospholipid...
January 10, 2018: International Journal of Systematic and Evolutionary Microbiology
Yang Cheng, Zhe Lin, Ju-Mei Wang, Long-Sheng Xing, Guang-Hua Xiong, Zhen Zou
C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. Immunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph...
February 14, 2018: Developmental and Comparative Immunology
A Ricci, V Racioppi, B Iotti, A Bertero, K F Reed, O B Pascottini, L Vincenti
Dystocic parturitions have an adverse impact on animal productivity and therefore the profitability of the farm. In this regard, accurate prediction of calving is essential since it allows for efficient and prompt assistance of the dam and the calf. Numerous approaches to predict parturition have been studied, among these, measurement of intravaginal temperature (IVT) is the most effective method at the field level. Thus, objectives of this experiment were, 1) to find an IVT cut-off to predict calving within 24 h, and 2) to clarify the use of IVT as an automated method of calving detection in housed beef cows...
February 10, 2018: Theriogenology
Anelize Bauermeister, Felipe A Calil, Francisco das C L Pinto, Talita C T Medeiros, Larissa C Almeida, Leonardo J Silva, Itamar S de Melo, Tiago D Zucchi, Letícia V Costa-Lotufo, Luiz A B Moraes
A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments...
February 16, 2018: Natural Product Research
Andriana I Papaioannou, Elisavet Konstantelou, Anastasia Papaporfyriou, Konstantinos Bartziokas, Aris Spathis, Petros Bakakos, Stelios Loukides, Nikolaos Koulouris, Spyros Papiris, Konstantinos Kostikas
Surfactant proteins (SPs) have been studied in COPD patients as biomarkers of disease severity and as predictive factors of unfavorable outcomes. The aim of this exploratory study was to evaluate serum levels of SP-A, SP-B, SP-C, and SP-D in patients with COPD both during AECOPD and in stability and to test their possible associations with disease severity and with the development of new exacerbation events. 20 consecutive COPD patients hospitalized for AECOPD were included. Serum SP levels were measured on admission, at discharge, and on stability...
February 14, 2018: Lung
Izelmar Todero, Tássia C Confortin, Juliana F Soares, Thiarles Brun, Luciana Luft, Jéssica E Rabuske, Raquel C Kuhn, Marcus V Tres, Giovani L Zabot, Marcio A Mazutti
This study is focused on the concentration of fermented broth from Phoma sp. to increase its herbicidal activity. For this purpose, biomolecules produced by submerged fermentation using Phoma sp. were concentrated by hollow fiber microfiltration membranes. The membrane feed was separated into two streams (retentate and permeate) and the crude broth was concentrated to 10, 30, 50, 70 and 90% (relative to the initial volume). The retentate samples were submitted to bioassays (triplicate) for evaluating their phytotoxic effects on five young leaves of species of Cucumis sativus and also on pre-emergence of weeds as Bidens pilosa and Amaranthus retroflexus...
February 14, 2018: Environmental Technology
C L Wang, Z X Xia, G H Zhang, J B You, H Yu, L L Wang, M Z Zhang, H B Yang, J P Gu, Y K Jin
OBJECTIVES: To observe the protein expression patterns of matrix metalloproteinase (MMP)-2 and MMP-9 in the liver tissue of liver contusion rats at different time after impact. METHODS: Fifty healthy adult male SD rats were randomly and evenly divided into control group and experimental groups (1 h, 3 h, 6 h, 12 h, 18 h, 24 h, 3 d, 5 d, 7 d after liver contusion). A rat liver contusion model was established by a free-falling device. The rats were killed at corresponding time, and the contused hepatic lobes were extracted...
December 2017: Fa Yi Xue za Zhi
F N Morgado, L M V de Carvalho, J Leite-Silva, A J Seba, M I F Pimentel, A Fagundes, M F Madeira, M R Lyra, M M Oliveira, A O Schubach, F Conceição-Silva
The clinical presentations of skin diseases produced by different pathogens, as American tegumentary leishmaniasis (ATL) and sporotrichosis can be similar and possibly influenced by the skin immune system (SIS). The aim of the study was to understand the underlying mechanisms of skin inflammation produced by different pathogens. We used immunohistochemistry to analyze 96 patients: a- localized cutaneous leishmaniasis (LCL-ATL); b- sporotrichoid cutaneous leishmaniasis (SCL-ATL); c-lymphocutaneous (LC-SP); d- fixed (F-SP) sporotrichosis...
February 13, 2018: Scientific Reports
Isabel Egea, Irene Albaladejo, Victoriano Meco, Belén Morales, Angel Sevilla, Maria C Bolarin, Francisco B Flores
Breeding for drought-tolerant crops is a pressing issue due to the increasing frequency and duration of droughts caused by climate change. Although important sources of variation for drought tolerance exist in wild relatives, the mechanisms and the key genes controlling tolerance in tomato are little known. The aim of this study is to determine the drought response of the tomato wild relative Solanum pennellii (Sp) compared with the cultivated tomato Solanum lycopersicum (Sl). The paper investigates the physiological and molecular responses in leaves of Sp and Sl plants without stress and moderate drought stress...
February 12, 2018: Scientific Reports
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