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Patricia C R Rabelo, Letícia Maria Souza Cordeiro, Nayara Soares Sena Aquino, Bruno B B Fonseca, Cândido Celso Coimbra, Samuel Penna Wanner, Raphael Escorsim Szawka, Danusa Dias Soares
The present study investigated whether intrinsic exercise capacity affects the changes in thermoregulation, metabolism and central dopamine (DA) induced by treadmill running. Male Wistar rats were subjected to three incremental exercises and ranked as low-performance (LP), standard-performance (SP) and high-performance (HP) rats. In the first experiment, abdominal (TABD ) and tail (TTAIL ) temperatures were registered in these rats during submaximal exercise (SE) at 60% of maximal speed. Immediately after SE, rats were euthanized and concentrations of DA and 3,4-dihydroxyphenylacetic acid (DOPAC) were determined in the preoptic area (POA)...
June 21, 2018: Journal of Applied Physiology
Mohammad Javed Ali, Nadimpalli Siva Kumar, Lars Bräuer, Friedrich Paulsen, Martin Schicht
PURPOSE: To investigate the presence and distribution patterns of 6 surfactant proteins (SPs) in the human lacrimal canaliculus. METHODS: The study was performed on fresh frozen cadaveric samples of canaliculi. Immunohistochemical labeling was performed for assessing the presence and distribution of SP: SP-A, SP-B, SP-C, SP-D, SP-G/SFTA2, and SP-H/SFTA3. Immunofluorescence double staining was performed using the respective fluorescein-conjugated antibodies and the results were scored as positive or negative and the distribution pattern within the canalicular system was assessed...
June 18, 2018: Ophthalmic Plastic and Reconstructive Surgery
Rafaela F Amatuzzi, Carolina G Poitevin, Alex S Poltronieri, Maria A C Zawadneak, Ida C Pimentel
Duponchelia fovealis (Lepidoptera: Crambidae) is an invasive species that has had a large impact on strawberry crops in Brazil. Pesticides have had limited effectiveness and the use of biological control agents to improve its management is the most appropriate approach. The aim of this study was to evaluate the pathogenicity and virulence of entomopathogenic fungi—isolated from soil—against Duponchelia fovealis larvae under laboratory and greenhouse conditions. Pathogenicity screenings were performed for twenty isolates from Beauveria bassiana , Beauveria caledonica , Isaria javanica , Metarhizium anisopliae , and Lecanicillium sp...
June 19, 2018: Insects
G B Slobodkina, R V Baslerov, N K Kostryukova, E A Bonch-Osmolovskaya, A I Slobodkin
A novel moderately thermophilic, anaerobic bacterium, strain STR9T was isolated from terrestrial hot spring at Uzon Caldera, Kamchatka peninsula, Russia. Cells of strain STR9T were spore-forming motile straight or slightly curved rods 0.3 μm in diameter and 1.0-2.0 μm in length having a Gram-positive cell-wall structure. The temperature range for growth was 30-58 °C, with an optimum at 50 °C. The pH range for growth was 5.0-10.5, with an optimum at pH 8.0-9.0. NaCl inhibited growth of the strain STR9T and no growth was evident above 0...
June 18, 2018: Extremophiles: Life Under Extreme Conditions
Esteban Soto, Matt J Griffin, Juan Alberto Morales, Elías Barquero Calvo, Fernanda de Alexandre Sebastião, Adrián Lopez Porras, Xindy Víquez-Rodríguez, Stephen R Reichley, Thomas G Rosser, Cynthia Ware, Barbara A Byrne, Julio C García, Benjamin R LaFrentz, Alvin C Camus
Historically, piscine francisellosis in various warm, temperate and coldwater fish hosts has been attributed to Francisella noatunensis From 2015-2016, an undescribed Francisella sp. was recovered during mortality events in cultured spotted rose snapper ( Lutjanus guttatus ) off the Pacific coast of Central America. Despite high mortality and emaciation, limited gross findings were observed in affected fish. Histological examination revealed multifocal granulomatous lesions, with the presence of numerous small, pleomorphic coccobacilli, predominantly in the peritoneum, spleen, kidneys, liver, pancreas, heart, and intestine...
June 18, 2018: Applied and Environmental Microbiology
Villy Meineche-Schmidt
Background Economy is an important part of chronic pain. Aim To describe the economy in chronic non-malignant pain patients attending a 13-week Rehabilitation Program (RP). Methods All patients participating in the RP 2006-2008 were evaluated at baseline (BL) and at follow-up (FU) after an observation period of mean … month in relation to: (1) work-income (WI) or (2) transfer income (TI), comprised by: (a) sick-leave (SL), (b) sick pension (SP), (c) social benefit (SB) and (d) rehabilitation benefit (RB)...
December 29, 2017: Scandinavian Journal of Pain
Min Shen, Xin Ju, Xinqi Xu, Xuemei Yao, Liangzhi Li, Jiajia Chen, Cuiying Hu, Jiaolong Fu, Lishi Yan
In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of L-rhamnose to L-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions (50°C, pH 8...
May 8, 2018: Journal of Microbiology and Biotechnology
Ernesto Robayo Camacho, Juang-Horng Chong, S Kris Braman, Steven D Frank, Peter B Schultz
We documented the species composition, seasonal ecology, and impacts of parasitoids and predators of Parthenolecanium corni (Bouché) and P. quercifex (Fitch) (Hemiptera: Coccidae) in the urban landscapes of Georgia, North Carolina, South Carolina, and Virginia, United States. Twenty-one parasitoid morphospecies and 12 predator species were collected through rearing, beat sheet, and sticky card trapping. Coccophagus lycimnia (Walker) (Hymenoptera: Aphelinidae) was the most abundant parasitoid species in South Carolina, North Carolina, and Virginia, whereas Metaphycus sp...
April 18, 2018: Journal of Economic Entomology
Hao-Bing Yu, Heng Jiao, Yu-Ping Zhu, Jian-Peng Zhang, Xiao-Ling Lu, Xiao-Yu Liu
Two new cyclohexanone derivatives, nectriatones A-B (1-2), and one new natural product, nectriatone C (3), together with three known phenolic sesquiterpene derivatives (4-6), were isolated from the culture of Nectria sp. B-13 obtained from high-latitude soil of the Arctic. The structures of all compounds were unambiguously elucidated by extensive spectroscopic analysis, as well as by comparison with the literature. These compounds were evaluated in cytotoxic and antibacterial activities. Compounds 1-6 showed cytotoxicities against SW1990, HCT-116, MCF-7, and K562 cells, with IC50 values in the range of 0...
June 18, 2018: Journal of Asian Natural Products Research
Pedro F Malacarne, Thiago N Menezes, Cleciane W Martins, Gustavo B Naumann, Helena L Gomes, Rita G W Pires, Suely G Figueiredo, Fabiana V Campos
Proteins that account for the hemolytic activity found in scorpaeniform fish venoms are responsible for the majority of the effects observed upon envenomation, for instance, neurotoxic, cardiotoxic and inflammatory effects. These multifunctional toxins, described as protein lethal factors and referred to as cytolysins, are known to be extremely labile molecules. In the present work, we endeavored to overcome this constraint by determining optimal storage conditions for Sp-CTx, the major bioactive component from the scorpionfish Scorpaena plumieri venom...
June 11, 2018: Toxicon: Official Journal of the International Society on Toxinology
Paula Domingo, Maite Olaciregui, Noelia González, Ignacio DE Blas, Lydia Gil
The purpose of this research was to assess whether the presence of seminal plasma (SP) can improve sperm quality of rabbit spermatozoa stored at 16ºC for 72 hours and moreover evaluate the cryoprotectant effects of glycerol, N-N-Dimethylformamide (DMF), and N-methyl-2-pyrrolidone (NMP). Semen samples were pooled and divided in eight fractions. Four of them were diluted with INRA (extender A), INRA with 6% glycerol (extender B), INRA with 6% DMF (extender C), or INRA with 6% NMP (extender D), respectively. The other four fractions were centrifuged, and the supernatant was removed in order to eliminate SP...
June 13, 2018: Experimental Animals
Marco Agostoni, Alshaé R Logan-Jackson, Emily R Heinz, Geoffrey B Severin, Eric L Bruger, Christopher M Waters, Beronda L Montgomery
Second messengers are intracellular molecules regulated by external stimuli known as first messengers that are used for rapid organismal responses to dynamic environmental changes. Cyclic di-AMP (c-di-AMP) is a relatively newly discovered second messenger implicated in cell wall homeostasis in many pathogenic bacteria. C-di-AMP is synthesized from ATP by diadenylyl cyclases (DAC) and degraded by specific c-di-AMP phosphodiesterases (PDE). C-di-AMP DACs and PDEs are present in all sequenced cyanobacteria, suggesting roles for c-di-AMP in the physiology and/or development of these organisms...
2018: Frontiers in Microbiology
Y Zhang, Z Q Wang, X G Sun
Objective: To analyze the etiology, drug susceptibility and etiological changes of fungal keratitis so as to to provide reference for clinical diagnosis and treatment. Methods: Retrospective case series study. A total of 5 654 copies of specimens for fungal culture were collected from Outpatients suspected of fungal keratitis in Beijing Tongren Ophthalmic Center during January 2007 and December 2016. Specimens were inoculated on Potato dextrose agar (PDA) slant medium, incubated at 30℃ and 40% humidity constantly for 3 to 10 days...
June 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Margot Schlusselhuber, Justine Godard, Muriel Sebban, Benoit Bernay, David Garon, Virginie Seguin, Hassan Oulyadi, Nathalie Desmasures
Biosurfactants such as lipopeptides are amphiphilic compounds produced by microorganisms such as bacteria of the genera of Pseudomonas and Bacillus . Some of these molecules proved to have interesting antimicrobial, antiviral, insecticide, and/or tensioactive properties that are potentially useful for the agricultural, chemical, food, and pharmaceutical industries. Raw milk provides a physicochemical environment that is favorable to the multiplication of a broad spectrum of microorganisms. Among them, psychrotrophic bacterial species, especially members of the genus Pseudomonas , are predominant and colonize milk during cold storage and/or processing...
2018: Frontiers in Microbiology
Donald P Knowles, Lowell S Kappmeyer, Darrell Haney, David R Herndon, Lindsay M Fry, James B Munro, Kelly Sears, Massaro W Ueti, Lauren N Wise, Marta Silva, David A Schneider, Juanita Grause, Stephen N White, Kyle Tretina, Richard P Bishop, David O Odongo, Angela M Pelzel-McCluskey, Glen A Scoles, Robert H Mealey, Joana C Silva
A novel apicomplexan parasite was serendipitously discovered in horses at the United States - Mexico border. Phylogenetic analysis based on 18S rDNA showed the erythrocyte-infective parasite to be related to, but distinct from, Theileria spp. in Africa, the most similar taxa being Theileria spp. from waterbuck and mountain zebra. The degree of sequence variability observed at the 18S rDNA locus also suggests the likely existence of additional cryptic species. Among described species, the genome of this novel equid Theileria parasite is most similar to that of Theileria equi, also a pathogen of horses...
June 6, 2018: International Journal for Parasitology
T B Shiell, D G McCulloch, D R McKenzie, M R Field, B Haberl, R Boehler, B A Cook, C de Tomas, I Suarez-Martinez, N A Marks, J E Bradby
Glassy carbon is a technologically important material with isotropic properties that is nongraphitizing up to ∼3000 °C and displays complete or "superelastic" recovery from large compression. The pressure limit of these properties is not yet known. Here we use experiments and modeling to show permanent densification, and preferred orientation occurs in glassy carbon loaded to 45 GPa and above, where 45 GPa represents the limit to the superelastic and nongraphitizing properties of the material...
May 25, 2018: Physical Review Letters
Mariana R Lopes, Carla A Lara, Marina E F Moura, Ana Paula T Uetanabaro, Paula B Morais, Marcos J S Vital, Carlos A Rosa
We investigated the yeast species associated with rotting wood samples obtained from Brazilian ecosystems, with a special focus on cellobiose-fermenting species. About 647 yeast strains were isolated from rotting wood samples collected from the areas of Atlantic rainforest, Cerrado, and Amazonian forest. Eighty-six known species and 47 novel species of yeasts were isolated. Candida boidinii, Cyberlindnera subsufficiens, Meyerozyma guilliermondii, Schwanniomyces polymorphus, Candida natalensis, and Debaryomyces hansenii were the most frequently isolated species...
July 2018: Fungal Biology
Hyun Kim, Ae Kyung Park, Jun Hyuck Lee, Seung Chul Shin, Hyun Park, Han Woo Kim
Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Alaska. PsEst3 was successfully overexpressed using a psychrophilic chaperonin co-expression system and was purified by nickel-affinity and size-exclusion chromatography. Recombinant PsEst3 was crystallized at 290 K using the hanging-drop vapour-diffusion method...
June 1, 2018: Acta Crystallographica. Section F, Structural Biology Communications
Zaki Saati-Santamaría, Rubén López-Mondéjar, Alejandro Jiménez-Gómez, Alexandra Díez-Méndez, Tomáš Větrovský, José M Igual, Encarna Velázquez, Miroslav Kolarik, Raúl Rivas, Paula García-Fraile
Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments for microbial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs. Bark beetles live in phloem of host trees and establish symbioses with microorganisms that provide them with nutrients. In addition, some of their associated bacteria play a role in the beetle protection by producing substances that inhibit antagonists...
2018: Frontiers in Microbiology
Kazutaka Takeshita, Hideyuki Tamaki, Tsubasa Ohbayashi, Xian-Ying Meng, Teruo Sone, Yasuo Mitani, Charlotte Peeters, Yoshitomo Kikuchi, Peter Vandamme
A Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacterium, designated strain RPE64T , was isolated from the gut symbiotic organ of the bean bug Riptortus pedestris, collected in Tsukuba, Japan, in 2007. 16S rRNA gene sequencing showed that this strain belongs to the Burkholderia glathei clade, exhibiting the highest sequence similarity to Burkholderia peredens LMG 29314T (100 %), Burkholderia turbans LMG 29316T (99.52 %) and Burkholderia ptereochthonis LMG 29326T (99.04 %). Phylogenomic analyses based on 107 single-copy core genes and Genome blast Distance Phylogeny confirmed B...
June 4, 2018: International Journal of Systematic and Evolutionary Microbiology
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