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https://www.readbyqxmd.com/read/27918491/clarification-of-the-antagonistic-effect-of-the-lipopeptides-produced-by-bacillus-amyloliquefaciens-bpd1-against-pyricularia-oryzae-via-in-situ-maldi-tof-ims-analysis
#1
Jen-Hung Liao, Pi-Yu Chen, Yu-Liang Yang, Shu-Chen Kan, Feng-Chia Hsieh, Yung-Chang Liu
This study tried to clarify the antagonistic effect of the lipopeptides secreted by Bacillus amyloliquefaciens strain BPD1 (Ba-BPD1) against Pyricularia oryzae Cavara (PO). To determine the major antifungal lipopeptides effective against PO, single and dual cultures were carried out in solid-state media. The matrix-assisted laser desorption/ionization-time of flight imaging mass spectrometry (MALDI-TOF IMS) was used to identify the most effective lipopeptide in situ. Meanwhile, the morphology of pathogen fungi treated with lipopeptides was observed via the SEM...
December 3, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27918241/detection-of-oil-palm-root-penetration-by-agrobacterium-mediated-transformed-ganoderma-boninense-expressing-green-fluorescent-protein
#2
Nisha Thopla Govender, Mui-Yun Wong
A highly efficient and reproducible Agrobacterium-mediated transformation protocol for Ganoderma boninense was developed to facilitate observation of the early stage infection of basal stem rot (BSR). The method was proven amenable to different explants (basidiospore, protoplast and mycelium) of G. boninense. The transformation efficiency was highest (62%) under a treatment combination of protoplast explants and Agrobacterium strain LBA 4404 with successful expression of hygromycin (hyg) marker gene and gus-gfp fusion gene, under the control of heterologous p416 gpd promoter...
December 5, 2016: Phytopathology
https://www.readbyqxmd.com/read/27916794/a-jacalin-related-lectin-regulated-the-formation-of-aerial-mycelium-and-fruiting-body-in-flammulina-velutipes
#3
Yuan-Ping Lu, Ren-Liang Chen, Ying Long, Xiao Li, Yu-Ji Jiang, Bao-Gui Xie
Flammulina velutipes, one of the most popular mushroom species in the world, has been recognized as a useful model system to study the biochemical and physiological aspects of the formation and elongation of fruit body. However, few reports have been published on the regulation of fruiting body formation in F. velutipes at the molecular level. In this study, a jacalin-related lectin gene from F. velutipes was characterized. The phylogenetic tree revealed that Fv-JRL1 clustered with other basidiomycete jacalin-like lectins...
November 28, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27910792/optimization-of-liquid-fermentation-conditions-and-protein-nutrition-evaluation-of-mycelium-from-the-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes
#4
Jie Gang, Han Liu, Yanhong Liu
Cordyceps militaris is a well-known traditional Chinese medicinal mushroom. In this study, the mycelium of C. militaris was cultured using liquid fermentation technology and the culture medium components were optimized by the orthogonal test method. Our results showed that the optimal medium combination for the mycelium growth is 3% glucose, 3% peptone, 0.1% MgSO4, and 0.2% KH2PO4 The international general nutritional assessment method was applied to the overall evaluation of the protein nutrition value of submerged cultivated mycelium and fruit body of C...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910787/nonvolatile-taste-components-and-antioxidant-properties-of-fruiting-body-and-mycelium-with-high-ergothioneine-content-from-the-culinary-medicinal-golden-oyster-mushroom-pleurotus-citrinopileatus-agaricomycetes
#5
Shin-Yi Lin, Shih-Chang Chien, Sheng-Yang Wang, Jeng-Leun Mau
Pleurotus citrinopileatus mycelium was prepared with high ergothioneine (Hi-Ergo) content and its proximate composition, nonvolatile taste components, and antioxidant properties were studied. The ergothioneine contents of fruiting bodies and Hi-Ergo and regular mycelia were 3.89, 14.57, and 0.37 mg/g dry weight, respectively. Hi-Ergo mycelium contained more dietary fiber, soluble polysaccharides, and ash but less carbohydrates, reducing sugar, fiber, and fat than regular mycelium. However, Hi-Ergo mycelium contained the smallest amounts of total sugars and polyols (47...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27904408/quality-of-bread-supplemented-with-antrodia%C3%A2-salmonea-fermented-grains
#6
Rao-Chi Chien, Enkhjargal Ulziijargal, Jeng-Leun Mau
Fermented grains of buckwheat, oat, embryo rice and wheat, which were prepared by solid-state fermentation with Antrodia salmonea, and the mycelium was used to substitute 7% of wheat flour to make bread. No difference in proximate composition, texture profile and contents of non-volatile taste components was observed among bread samples. White bread and bread supplemented with mycelium and fermented grains looked different. Bread supplemented with fermented grains had similar thermal properties, which differed from those of white bread and bread supplemented with mycelium...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27902245/streptomyces-luteus-sp-nov-a-novel-actinomycete-isolated-from-loulan-soil-of-xinjiang-china
#7
Xiaoxia Luo, Kai Liang, Yang Wang, Chuanxing Wan, Lili Zhang
A Streptomyces strain, designated TRM 45540T, was isolated from the soil of Loulan area(89°22'22″E, 40°29'55″N), Xinjiang Province, north-west China and was characterized taxonomically by using a polyphasic study. Phylogenetic analysis of the strain from 16S rRNA sequence revealed the strain TRM 45540 had 99.87% similarity with the type strain Streptomyces mutabilis NRRL12800T (GenBank accession number. AB184156). The strain was aerobic, gram-staining-positive, with an optimum NaCl concentration for growth of 5% (w/v)...
November 1, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27898038/a-method-for-determining-the-content-of-glycoproteins-in-biological-samples
#8
Yang Gao, Duoduo Xu, Hongyue Li, Xianling Yang, Mingxing Wang, Qipin Gao
The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs...
November 26, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27896685/the-interactions-of-vanadate-monomer-with-the-mycelium-of-fungus-phycomyces-blakesleeanus-reduction-or-uptake
#9
Mirzeta Hadžibrahimović, Desanka Sužnjević, Ferenc Pastor, Tijana Cvetić Antić, Milan Žižić, Joanna Zakrzewska, Miroslav Živić
The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 µmol/gFW, and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation...
November 28, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27896349/silver-nanoparticles-a-mechanism-of-action-on-moulds
#10
Katarzyna Pietrzak, Sława Glińska, Magdalena Gapińska, Tomasz Ruman, Adriana Nowak, Egemen Aydin, Beata Gutarowska
Silver nanoparticles (AgNPs) are widely used in all branches of industry. However, their mechanisms of action towards moulds have not been studied yet. Thus we conducted this study in which we have used laser desorption/ionization time-of-flight mass spectrometry (LDI-ToF-MS) analysis to determine metabolomic changes, and microscopic analysis (transmission electron microscopy, fluorescent microscopy) to observe changes in mould cells. The AgNP treatment caused the downregulation of 162 (15 ppm) and 284 (62 ppm), and 19 (15 ppm) and 29 (62 ppm) metabolites of Aspergillus niger and Penicillium chrysogenum, respectively...
November 29, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27889421/production-of-native-arbuscular-mycorrhizal-fungi-inoculum-under-different-environmental-conditions
#11
Yamir Torres-Arias, Rosalba Ortega Fors, Camila Nobre, Eduardo Furrazola Gómez, Ricardo Luis Louro Berbara
In order to obtain an arbuscular mycorrhizal fungi (AMF) native inoculum from Sierra de Moa and determine the most appropriate conditions for its big scale production, four light and temperature combinations were tested in three plant species (Calophyllum antillanum, Talipariti elatum and Paspalum notatum). Growth and development parameters, as well as the mycorrhizal functioning of the seedlings were evaluated. The natural light treatment under high temperatures (L-H) was the most suitable for the growth and development of the three plant species, showing the highest total biomass values, mainly of root, and a positive root-shoot ratio balance...
November 8, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27879685/synthesis-and-biological-evaluation-of-benzimidazole-phenylhydrazone-derivatives-as-antifungal-agents-against-phytopathogenic-fungi
#12
Xing Wang, Yong-Fei Chen, Wei Yan, Ling-Ling Cao, Yong-Hao Ye
A series of benzimidazole phenylhydrazone derivatives (6a-6ai) were synthesized and characterized by ¹H-NMR, ESI-MS, and elemental analysis. The structure of 6b was further confirmed by single crystal X-ray diffraction as (E)-configuration. All the compounds were screened for antifungal activity against Rhizoctonia solani and Magnaporthe oryzae employing a mycelium growth rate method. Compound 6f exhibited significant inhibitory activity against R. solani and M. oryzae with the EC50 values of 1.20 and 1.85 μg/mL, respectively...
November 22, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27873003/localization-of-ergot-alkaloids-in-sclerotia-of-claviceps-purpurea-by-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
#13
Julian Dopstadt, Simeon Vens-Cappell, Lisa Neubauer, Paul Tudzynski, Benedikt Cramer, Klaus Dreisewerd, Hans-Ulrich Humpf
The fungus Claviceps purpurea produces highly toxic ergot alkaloids and accumulates these in the hardened bodies of fungal mycelium. These so-called sclerotia, or ergot bodies, replace the crop seed of infected plants, which can include numerous important food- and feedstuff such as rye and wheat. While several studies have explored details of the infection process and development of ergot bodies, little information is available on the spatial distribution of the mycotoxins in the sclerotia. Here we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) at a lateral resolution of 35 μm to visualize the distribution of two representative alkaloids, ergocristine and ergometrine, produced by Ecc93 and Gal 310 variants of C...
November 21, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27871132/aspergillus-penicillioides-differentiation-and-cell-division-at-0-585-water-activity
#14
Andrew Stevenson, Philip G Hamill, Callum J O'Kane, Gerhard Kminek, John D Rummel, Mary A Voytek, Jan Dijksterhuis, John E Hallsworth
Water availability acts as the most-stringent constraint for life on Earth. Thus, understanding the water relations of microbial extremophiles is imperative to our ability to increase agricultural productivity (e.g., by enhancing the processing and turnover of dead organic matter in soils of arid regions); reduce human exposure to mycotoxins in buildings and our food-supply chain; prevent the spoilage of foods/animal feeds, books, museum specimens and artworks; and better control microbiology of industrial fermentations...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27868065/comparative-proteomic-analysis-of-light-induced-mycelial-brown-film-formation-in-lentinula-edodes
#15
Li Hua Tang, Qi Tan, Da Peng Bao, Xue Hong Zhang, Hua Hua Jian, Yan Li, Rui Heng Yang, Ying Wang
Light-induced brown film (BF) formation by the vegetative mycelium of Lentinula edodes is important for ensuring the quantity and quality of this edible mushroom. Nevertheless, the molecular mechanism underlying this phenotype is still unclear. In this study, a comparative proteomic analysis of mycelial BF formation in L. edodes was performed. Seventy-three protein spots with at least a twofold difference in abundance on two-dimensional electrophoresis (2DE) maps were observed, and 52 of them were successfully identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF/MS)...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27866250/itraq-based-comparative-proteomics-analysis-of-the-fruiting-dikaryon-and-the-non-fruiting-monokaryon-of-flammulina-velutipes
#16
Jing-Yu Liu, Ming-Chang Chang, Jun-Long Meng, Cui-Ping Feng, Yong-Nan Liu
Flammulina velutipes is a potentially excellent fungus to study basic mechanisms of basidiomycete mycelium biology. To provide a better understanding of the mechanism of hyphae growth and fruit-body formation, the biological functions of the differentially abundant proteins between the fruiting dikaryon and the non-fruiting monokaryon of F. velutipes were investigated at the proteomic level using iTRAQ-coupled two-dimensional liquid chromatography tandem mass spectrometry technique. Among the 1198 proteins identified with high confidence, a total of 472 proteins were detected differentially abundant at least one of the mycelium development stages...
November 19, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27859287/fungal-and-plant-gene-expression-in-the-tulasnella-calospora-serapias-vomeracea-symbiosis-provides-clues-about-nitrogen-pathways-in-orchid-mycorrhizas
#17
Valeria Fochi, Walter Chitarra, Annegret Kohler, Samuele Voyron, Vasanth R Singan, Erika A Lindquist, Kerrie W Barry, Mariangela Girlanda, Igor V Grigoriev, Francis Martin, Raffaella Balestrini, Silvia Perotto
Orchids are highly dependent on their mycorrhizal fungal partners for nutrient supply, especially during early developmental stages. In addition to organic carbon, nitrogen (N) is probably a major nutrient transferred to the plant because orchid tissues are highly N-enriched. We know almost nothing about the N form preferentially transferred to the plant or about the key molecular determinants required for N uptake and transfer. We identified, in the genome of the orchid mycorrhizal fungus Tulasnella calospora, two functional ammonium transporters and several amino acid transporters but found no evidence of a nitrate assimilation system, in agreement with the N preference of the free-living mycelium grown on different N sources...
November 11, 2016: New Phytologist
https://www.readbyqxmd.com/read/27842536/antibacterial-and-cytotoxic-cytochalasins-from-the-endophytic-fungus-phomopsis-sp-harbored-in-garcinia-kola-heckel-nut
#18
Jean-Bosco Jouda, Jean-de-Dieu Tamokou, Céline Djama Mbazoa, Clovis Douala-Meli, Prodipta Sarkar, Prasanta Kumar Bag, Jean Wandji
BACKGROUND: The continuous emergence of multidrug-resistant (MDR) bacteria drastically reduced the efficacy of our antibiotic armory and consequently, increased the frequency of therapeutic failure. The search for bioactive constituents from endophytic fungi against MDR bacteria became a necessity for alternative and promising strategies, and for the development of novel therapeutic solutions. We report here the isolation and structure elucidation of antibacterial and cytotoxic compounds from Phomopsis sp...
November 14, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27829359/selection-of-streptomyces-against-soil-borne-fungal-pathogens-by-a-standardized-dual-culture-assay-and-evaluation-of-their-effects-on-seed-germination-and-plant-growth
#19
Andrea Kunova, Maria Bonaldi, Marco Saracchi, Cristina Pizzatti, Xiaoyulong Chen, Paolo Cortesi
BACKGROUND: In the search for new natural resources for crop protection, streptomycetes are gaining interest in agriculture as plant growth promoting bacteria and/or biological control agents. Because of their peculiar life cycle, in which the production of secondary metabolites is synchronized with the development of aerial hyphae and sporulation, the commonly used methods to screen for bacterial antagonists need to be adapted. RESULTS: The dual culture assay was standardized in terms of inoculation timing of Streptomyces antagonist and pathogen, and growth rate of different fungal pathogens...
November 9, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27810261/effect-of-paecilomyces-lilacinus-trichoderma-harzianum-and-trichoderma-virens-fungal-extracts-on-the-hatchability-of-ancylostoma-eggs
#20
Bianca Delgado Menezes Hofstätter, Anelise Oliveira da Silva Fonseca, Fernando de Souza Maia Filho, Julia de Souza Silveira, Beatriz Maroneze Persici, Luciana Pötter, Andressa Silveira, Zaida Inês Antoniolli, Daniela Isabel Brayer Pereira
BACKGROUND: Ancylostoma species have demanded attention due to their zoonotic potential. The use of anthelmintics is the usual method to prevent environmental contamination by Ancylostoma eggs and larvae. Nematophagous fungi have been widely used in their biological control due to the fungus ability to capture and digest free nematode forms. AIMS: The aim of this study was to evaluate the effect of four different fungal extracts of Paecilomyces lilacinus (n=2), Trichoderma harzianum (n=1) and Trichoderma virens (n=1) isolates on the hatchability of Ancylostoma eggs...
October 31, 2016: Revista Iberoamericana de Micología
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