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https://www.readbyqxmd.com/read/29763681/manuka-honey-attenuates-oxidative-damage-induced-by-h-2-o-2-in-human-whole-blood-in-vitro
#1
Lada Živković, Vladan Bajić, Dragana Dekanski, Andrea Čabarkapa-Pirković, Francesca Giampieri, Massimiliano Gasparrini, Luca Mazzoni, Biljana Spremo Potparević
Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed...
May 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29749429/antibacterial-and-antioxidant-activity-of-different-types-of-honey-derived-from-mount-olympus-in-greece
#2
Dimitrios Stagos, Nikolaos Soulitsiotis, Christina Tsadila, Stamatina Papaeconomou, Charalampos Arvanitis, Alexandros Ntontos, Fani Karkanta, Soultana Adamou-Androulaki, Konstantinos Petrotos, Demetrios A Spandidos, Demetrios Kouretas, Dimitris Mossialos
The aim of the present study was to examine the antioxidant and antibacterial activity of 21 types of honey derived from Mount Olympus (Mt. Olympus), a region with great plant biodiversity. The antibacterial activity was examined against the growth of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) by the agar well diffusion assay and the determination of the minimum inhibitory concentration (MIC). The antioxidant activity was assessed by using the 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) and 2,2'‑azino‑bis(3‑ethylbenzothiazoline‑6‑sulphonic acid (ABTS•+) free radical scavenging assays...
May 4, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29738292/comparison-of-antimicrobial-activity-of-selected-commercially-available-wound-dressing-materials
#3
Piotr Szweda, Grzegorz Gorczyca, Robert Tylingo
OBJECTIVE: The aim of our study was to examine the antimicrobial potential of eight selected, commercially available wound dressings containing different antimicrobial agents: silver, chlorhexidine acetate, povidone-iodine, and manuka honey. METHOD: The materials were tested against four reference strains of bacteria: Staphylococcus aureus (PCM 2051), Staphylococcus epidermidis (PCM 2118), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (K12), using the disc diffusion-like method and a time-killing assay...
May 2, 2018: Journal of Wound Care
https://www.readbyqxmd.com/read/29718813/efficacy-of-honeydew-honey-and-blossom-honey-on-full-thickness-wound-healing-in-mice
#4
Takuto Sawazaki, Yukari Nakajima, Tamae Urai, Kanae Mukai, Megumi Ohta, Ikuko Kato, Azusa Kawaguchi, Yui Kinoshita, Yuri Kumagai, Ayaka Sakashita, Asuka Yamazaki, Toshio Nakatani
INTRODUCTION: The wound healing properties of honey, including blossom honey, are well known; however, the effects of honeydew honey during the wound healing process have not yet been investigated and thus remain unclear. OBJECTIVE: This study compares the effects of honeydew honey with those of blossom honey. MATERIALS AND METHODS: A total of 140 mice were divided into 2 control groups, which received either a hydrocolloid dressing (HCD; n = 22) or gauze (n = 22), and 4 experimental groups: honeydew honey (n = 23), Acacia honey (n = 23), Manuka honey (n = 22), and Japanese Pharmacopoeia honey (n = 28)...
April 20, 2018: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29645049/the-inhibitory-effect-of-manuka-honey-on-human-colon-cancer-hct-116-and-lovo-cell-growth-part-1-the-suppression-of-cell-proliferation-promotion-of-apoptosis-and-arrest-of-the-cell-cycle
#5
Sadia Afrin, Francesca Giampieri, Massimiliano Gasparrini, Tamara Y Forbes-Hernández, Danila Cianciosi, Patricia Reboredo-Rodriguez, Adolfo Amici, Josè L Quiles, Maurizio Battino
Numerous investigations have been made on plant phenolic compounds and cancer prevention in recent decades. Manuka honey (MH) represents a good source of phenolic compounds such as luteolin, kaempferol, quercetin, gallic acid and syringic acid. The aim of this work was to evaluate the chemopreventive effects of MH on human colon cancer HCT-116 and LoVo cells. Both cells were exposed to different concentrations of MH (0-20 mg mL-1 for HCT-116 cells and 0-50 mg mL-1 for LoVo cells) for 48 h to measure apoptosis and cell cycle arrest as well as apoptosis and cell cycle regulatory gene and protein expression...
April 12, 2018: Food & Function
https://www.readbyqxmd.com/read/29644357/the-inhibitory-effect-of-manuka-honey-on-human-colon-cancer-hct-116-and-lovo-cell-growth-part-2-induction-of-oxidative-stress-alteration-of-mitochondrial-respiration-and-glycolysis-and-suppression-of-metastatic-ability
#6
Sadia Afrin, Francesca Giampieri, Massimiliano Gasparrini, Tamara Y Forbes-Hernández, Danila Cianciosi, Patricia Reboredo-Rodriguez, Piera Pia Manna, Jiaojiao Zhang, Josè L Quiles, Maurizio Battino
Despite its high content of phenolic compounds, the chemopreventive activity of Manuka honey (MH) is still elusive. The aim of the present work was to evaluate the effects of MH on oxidative stress, antioxidant enzymes, cellular metabolism and the metastatic ability in HCT-116 and LoVo cells, paying particular attention to the molecular mechanisms involved. We observed a strong induction of oxidative stress after MH treatment since it augmented the accumulation of reactive oxygen species and increased the damage to proteins, lipids and DNA...
April 12, 2018: Food & Function
https://www.readbyqxmd.com/read/29576087/expression-of-oswnk9-in-arabidopsis-conferred-tolerance-to-salt-and-drought-stress
#7
Rakesh Manuka, Ankush Ashok Saddhe, Kundan Kumar
With No Lysine (WNK) kinase belongs to ser/thr protein kinase group in which conserved catalytic lysine (K) residue of subdomain II is shifted to subdomain I. In this study, we cloned full-length coding region of WNK9 from Oryza sativa (OsWNK9) and performed in silico studies to confirm the presence of all kinase signature regulatory elements. The transcript analysis revealed that OsWNK9 was strongly down regulated under salinity, drought and ABA stress in shoots. Constitutive expression of OsWNK9 in Arabidopsis thaliana imparted increased tolerance to salt, drought, and ABA stress...
May 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29575121/manuka-honey-and-methylglyoxal-increase-the-sensitivity-of-staphylococcus-aureus-to-linezolid
#8
Gabrielle Hayes, Nicole Wright, Sarah L Gardner, Calla L Telzrow, Andrew J Wommack, Patrick A Vigueira
The continued emergence and global spread of bacterial antibiotic resistance has fueled the search for novel antimicrobial agents and resistance-modifying compounds. Manuka honey has both antimicrobial properties and the ability to increase the efficacy of FDA-approved antibiotic drugs. Compared to other types of honey, manuka honey contains elevated levels of methylglyoxal, a small molecule that contributes to its antibacterial activity. Manuka honey has shown particular promise for the treatment of antibiotic-resistant Gram-positive organisms such as methicillin-resistant Staphylococcus aureus...
March 25, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29574906/the-in-vitro-antimicrobial-evaluation-of-commercially-essential-oils-and-their-combinations-against-acne
#9
A Orchard, S F van Vuuren, A Viljoen, Guy Kamatou
OBJECTIVE: The study investigated the efficacy of commercial essential oil combinations against the two pathogens responsible for acne with the aim to identify synergy and favourable oils to possibly use in a blend. METHODS AND MATERIALS: Antimicrobial activity was assessed using the minimum inhibitory concentration (MIC) assay against Staphylococcus epidermidis (ATCC 2223) and Propionibacterium acnes (ATCC 11827), and the fractional inhibitory concentration index (ΣFIC) was calculated...
March 24, 2018: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/29568170/effectiveness-of-three-mouthwashes-manuka-honey-raw-honey-and-chlorhexidine-on-plaque-and-gingival-scores-of-12-15-year-old-school-children-a-randomized-controlled-field-trial
#10
Richa Singhal, Mahantesh Siddibhavi, Roopali Sankeshwari, Pratibha Patil, Sagar Jalihal, Anil Ankola
Objectives: The aim of this study is to compare the effectiveness of three types of mouthwashes manuka honey (MH), raw honey (RH), and chlorhexidine (CHX) on plaque and gingival scores of 12-15-year-old government school children. Study Design: This study was a double-blind, randomized controlled field trial conducted in Belagavi city, India. Materials and Methods: One hundred and thirty-five government school children aged 12-15 years were randomly selected and allocated into three groups, RH, MH, and CHX mouthwash groups...
January 2018: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29564238/antimicrobial-activity-of-four-essential-oils-against-pigmenting-pseudomonas-fluorescens-and-biofilmproducing-staphylococcus-aureus-of-dairy-origin
#11
Francesca Pedonese, Filippo Fratini, Luisa Pistelli, Federica Maria Porta, Pierluigi Di Ciccio, Roberto Fischetti, Barbara Turchi, Roberta Nuvoloni
Essential oils (EOs) are mixtures of secondary metabolites of plant origin with many useful properties, among which the antimicrobial activity is also of interest for the food industry. EOs can exert their antimicrobial potential both directly, in food products and active packaging, and indirectly, as sanitizing and anti-biofilm agents of food facility surfaces. Aim of this research was to evaluate the antimicrobial activity of four EOs (bergamot, cinnamon, manuka and thyme) against Pseudomonas fluorescens and Staphylococcus aureus isolated from milk and dairy products...
October 20, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29549100/characterization-of-serratia-proteamaculans-strain-agr96x-encoding-an-anti-feeding-prophage-tailocin-with-activity-against-grass-grub-costelytra-giveni-and-manuka-beetle-pyronota-spp-larvae
#12
Mark R H Hurst, Amy Beattie, Sandra A Jones, Aurelie Laugraud, Chikako van Koten, Lincoln Harper
A highly virulent Serratia proteamaculans strain, AGR96X, exhibiting specific pathogenicity against larvae of the New Zealand grass grub ( Costelytra giveni ; Coleoptera: Scarabaeidae) and the New Zealand manuka beetle ( Pyronota festiva and P. setosa ; Coleoptera: Scarabaeidae), was isolated from a diseased grass grub larva. A 12-day median lethal dose of 4.89 ± 0.92 × 103 cells per grass grub larva was defined for AGR96X, and death occurred within 5--12 days following ingestion of a high bacterial dose...
March 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29528528/mapping-floral-resources-for-honey-bees-in-new-zealand-at-the-catchment-scale
#13
A-G E Ausseil, J R Dymond, L Newstrom
Honey bees require nectar and pollen from flowers: nectar for energy and pollen for growth. The demand for nectar and pollen varies during the year, with more pollen needed in spring for colony population growth, and more nectar needed in summer to sustain the maximum colony size and collect surplus nectar stores for winter. Sufficient bee forage is therefore necessary to ensure a healthy bee colony. Land-use changes can reduce the availability of floral resources suitable for bees, thereby increasing the susceptibility of bees to other stressors such as disease and pesticides...
March 12, 2018: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/29478537/biochemical-properties-antibacterial-and-cellular-antioxidant-activities-of-buckwheat-honey-in-comparison-to-manuka-honey
#14
Jianling Deng, Rui Liu, Qun Lu, Peiyan Hao, Anqi Xu, Jiuliang Zhang, Jun Tan
The biochemical properties of buckwheat honey, including contents of sugars, proteins, total phenols, methylglyoxal (MGO), minerals and phenolic compounds, were determined in comparison with those of manuka honey. Buckwheat honey has higher contents of sugars, proteins and total phenols but a lower content of MGO than manuka honey. Buckwheat honey contains abundant minerals involved in a number of vital functions of the human body as does manuka honey, and has even higher contents of Fe, Mn and Zn. In buckwheat honey, p-hydroxybenzoic acid, chlorogenic acid and p-coumaric acid are the dominant phenolic compounds...
June 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29373742/single-and-joint-antibacterial-activity-of-aqueous-garlic-extract-and-manuka-honey-on-extended-spectrum-beta-lactamase-producing-escherichia-coli
#15
Ainau R Idris, Seniyat L Afegbua
Background: Multidrug resistance and recent technological advances have renewed interest in natural product drug discovery from ancient remedies such as Allium sativum (garlic) and honey. This study assessed antibacterial activity of aqueous garlic extract (AGE) and Manuka honey on extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. Methods: Thirty clinical isolates of E. coli were collected and screened for ESBL production by double-disc synergy test...
October 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29348094/in-vitro-salmonella-typhi-biofilm-formation-on-gallstones-and-its-disruption-by-manuka-honey
#16
Abdul Hannan, Asma Ejaz Bajwa, Saira Riaz, Usman Arshad, Sidrah Saleem, Usama Ijaz Bajwa
Biofilm is a complex community of single or different types of microorganisms (bacteria, viruses, fungi, protozoa) attached to a surface and stick to each other through production of extracellular matrix. Salmonella typhi forms biofilm on cholesterol gallstones resulting in carrier state. Once formed, biofilm is difficult to treat. To date cholecystectomy is the only cure for this condition. Manuka honey is known to have tremendous antibiofilm activity against various organisms. S. typhi biofilm was grown in vitro on clinical samples of human cholesterol gallstones by Gallstone tube assay method for 12 days...
January 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29304019/characterization-of-physico-chemical-properties-and-antioxidant-capacities-of-bioactive-honey-produced-from-australian-grown-agastache-rugosa-and-its-correlation-with-colour-and-poly-phenol-content
#17
Sushil Anand, Edwin Pang, George Livanos, Nitin Mantri
The antioxidant and antimicrobial components of honey vary based on sourced of nectar. Medicinal plants with the therapeutic value have potential to produce honey with greater bioactivity. The aim of the present study was to characterize the physico-chemical and antioxidant capacities of Agastache honey produced from Agastache rugosa and compare them with other popular commercial honeys sold in Australia. The total phenolics, total flavonoids, moisture content, colour, pH, protein content and antioxidant capacity were evaluated for Agastache , Manuka, Jelly bush, Tea tree, Super manuka and Jarrah honeys...
January 5, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29300875/influence-of-genotype-floral-stage-and-water-stress-on-floral-nectar-yield-and-composition-of-m%C3%A4-nuka-leptospermum-scoparium
#18
Michael J Clearwater, Maria Revell, Stevie Noe, Merilyn Manley-Harris
Background and Aims: Floral nectar can be variable in composition, influencing pollinator behaviour and the composition of honey derived from it. The non-peroxide antibacterial activity of mānuka (Leptospermum scoparium, Myrtaceae) honey results from the chemical conversion of the triose sugar dihydroxyacetone (DHA), after DHA accumulates for an unknown reason in the nectar. This study examined variation in nectar DHA, glucose, fructose and sucrose content with floral stage of development, between mānuka genotypes with differing flower morphology, and in response to water stress...
March 5, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29272983/sub-inhibitory-concentration-of-essential-oils-induces-antibiotic-resistance-in-staphylococcus-aureus
#19
Barbara Turchi, Simone Mancini, Luisa Pistelli, Basma Najar, Filippo Fratini
Fourteen Staphylococcus aureus wild strains were stressed with sub-inhibitory concentration of five essential oils: Leptospermum scoparium (manuka), Origanum majorana (marjoram), Origanum vulgare (oregano), Satureja montana (winter savoury) and Thymus vulgaris (thyme). Antibiotics susceptibility profiles of the strains were determined by agar disk diffusion method before and after EOs treatment. The following antibiotics were employed: amoxicillin-clavulanic acid, amikacin, ampicillin, amoxicillin, aztreonam, ceftazidime, cephalothin, ciprofloxacin, colistin, cefotaxime, doxycycline, enrofloxacin, erythromycin, gentamicin, cephalexin, neomycin, piperacillin, rifampin, streptomycin, trimethoprim-sulphamethoxazole, tetracycline and tobramycin...
December 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29232982/antioxidant-and-antimicrobial-properties-of-casteanea-sativa-miller-chestnut-honey-produced-on-mount-etna-sicily
#20
Simone Ronsisvalle, Edmondo Lissandrello, Virginia Fuochi, Giulio Petronio Petronio, Claudia Straquadanio, Lucia Crascì, Annamaria Panico, Marcella Milito, Anna Maria Cova, Gianna Tempera, Pio Maria Furneri
The aim of this study was the evaluation of antibacterial and antioxidant properties of Monofloral Etna Castanea sativa Miller honeys. Escherichia coli ATCC 25,922, Pseudomonas aeruginosa ATCC 27,853, Enterococcus faecalis ATCC 29,211 and Staphylococcus aureus ATCC 29,213 were investigated for their susceptibilities to two different honeys. Antioxidant activity was evaluated by ORAC, NO scavenger assays, FRAP and DPPH. Antioxidant activity and antibacterial properties were compared with chestnut honeys from different geographical areas and with Manuka honey...
December 13, 2017: Natural Product Research
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