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Victoria S Lee, Ian M Humphreys, Patricia L Purcell, Greg E Davis
BACKGROUND: Manuka honey (MH) has been shown in vitro to be effective against biofilm-producing bacteria. This study assessed the effectiveness of MH for patients with active chronic rhinosinusitis (CRS) and prior sinus surgery. METHODS: This prospective single-blinded (clinician only) randomized controlled trial recruited patients with active CRS and prior sinus surgery. Patients received either MH or saline (SAL) sinus irrigations twice daily for 30 days and were offered oral antibiotics and/or oral/topical steroids as indicated...
December 9, 2016: International Forum of Allergy & Rhinology
Fang Fang, Kerdalidec Candy, Elise Melloul, Charlotte Bernigaud, Ling Chai, Céline Darmon, Rémy Durand, Françoise Botterel, Olivier Chosidow, Arezki Izri, Weiyi Huang, Jacques Guillot
BACKGROUND: The development of alternative approaches in ectoparasite management is currently required. Essential oils have been demonstrated to exhibit fumigant and topical toxicity to a number of arthropods. The aim of the present study was to assess the potential efficacy of ten essential oils against Sarcoptes scabiei. METHODS: The major chemical components of the oils were identified by GC-MS analysis. Contact and fumigation bioassays were performed on Sarcoptes mites collected from experimentally infected pigs...
November 22, 2016: Parasites & Vectors
Nicole Beitlich, Tilo Lübken, Martin Kaiser, Lilit Ispiryan, Karl Speer
New Zealand manuka honey is well-known for its unique antibacterial activity. Due to its high price and limited availability, this honey is often subject to honey fraud. Two pteridine derivatives, 3,6,7-trimethyl-2,4(1H,3H)-pteridinedione and 6,7-dimethyl-2,4(1H,3H)-pteridinedione, have now been identified in New Zealand manuka honey. Their structures were elucidated by LC-QTOF-HRMS, NMR, and single-crystal X-ray diffraction after isolation via semi-preparative HPLC. Their marker potential for authentic manuka honey was proved as both substances were neither detectable in the pollen-identical kanuka honey nor in the nine other kinds of monofloral New Zealand honey analyzed (clover, forest, kamahi, pohutukawa, rata, rewarewa, tawari, thyme, and vipers bugloss)...
November 3, 2016: Journal of Agricultural and Food Chemistry
Marika Tenci, Silvia Rossi, Maria Cristina Bonferoni, Giuseppina Sandri, Irene Mentori, Cinzia Boselli, Antonia Icaro Cornaglia, Maria Daglia, Anna Marchese, Carla Caramella, Franca Ferrari
The aim of the present work was the development of a powder formulation for the delivery of manuka honey (MH) bioactive components in the treatment of chronic skin ulcers. In particular pectin (PEC)/chitosan glutamate (CS)/hyaluronic acid (HA) mini-capsules were obtained by inverse ionotropic gelation in presence of calcium chloride and subsequently freeze-dried. Optimization of unloaded (blank) formulation was performed using DoE approach. In a screening phase, the following three factors were investigated at two levels: CS (0...
October 24, 2016: International Journal of Pharmaceutics
(no author information available yet)
Manuka honey curbs the growth of bacterial biofilms even at low dilutions, new research suggests.
October 26, 2016: Nursing Standard
Niloufar Roshan, Thomas Rippers, Cornelia Locher, Katherine A Hammer
The physicochemical parameters and antibacterial activity of 10 Western Australian (WA) and two comparator honeys were determined. Honeys showed a pH range of 4.0-4.7, colour range of 41.3-470.7 mAU, methylglyoxal levels ranging from 82.2 to 325.9 mg kg(-1) and hydrogen peroxide levels after 2 h of 22.7-295.5 µM. Antibacterial activity was assessed by the disc diffusion assay, phenol equivalence assay, determination of minimum inhibitory and bactericidal concentrations and a time-kill assay. Activity was shown for all honeys by one or more method, however, activity varied according to which assay was used...
October 26, 2016: Archives of Microbiology
Nor Hayati Othman, Sarfraz Ahmed, Siti Amrah Sulaiman
No abstract text is available yet for this article.
February 2016: Pathology
Mohamed M M Abdel-Latif, Mekky M Abouzied
BACKGROUND AND AIMS: Natural honey has been used as a medicine since ancient times. Honey is widely known for its antibacterial properties against H. pylori; however, the mechanisms of its antibacterial activity are not fully known. The present study was performed to examine the molecular mechanisms by which natural honey can inhibit H. pylori infection in gastric epithelial cells. METHODS: Electrophoretic mobility shift assay was used to measure NF-κB- and AP-1-DNA binding activity...
July 2016: Archives of Medical Research
Wisnu Adi Wicaksono, E Eirian Jones, Jana Monk, Hayley J Ridgway
Leptospermum scoparium or mānuka is a New Zealand native medicinal plant that produces an essential oil with antimicrobial properties. This is the first study to investigate the structure and bioactivity of endophytic bacteria in mānuka by using a combination of cultivation-independent (DGGE) and dependent approaches. A total of 23 plants were sampled across three sites. Plants were considered either immature (3-8 years) or mature (>20 years). The endophyte community structure and richness was affected by plant tissue and bacterial communities became more stable and uniform as plant maturity increased...
2016: PloS One
Somadina Emineke, Alan J Cooper, Sarah Fouch, Brian R Birch, Bashir A Lwaleed
AIMS: Biofilms are ubiquitous and when mature have a complex structure of microcolonies in an extracellular polysaccharide and extracellular DNA matrix. Indwelling medical devices harbour biofilms which have been shown to cause infections and act as reservoirs for pathogens. Urinary catheters are often in place for considerable periods of time and are susceptible to both encrustation and biofilm formation. Strategies for minimising biofilm occurrence underpin an active research area in biomedicine...
September 26, 2016: Journal of Clinical Pathology
Marc Spiteri, Karyne M Rogers, Eric Jamin, Freddy Thomas, Sophie Guyader, Michèle Lees, Douglas N Rutledge
Manuka honey is a product produced essentially in New Zealand, and has been widely recognised for its antibacterial properties and specific taste. In this study, 264 honeys from New Zealand and Australia were analysed using proton NMR spectroscopy coupled with chemometrics. Known manuka markers, methylglyoxal and dihydroxyacetone, have been characterised and quantified, together with a new NMR marker, identified as being leptosperin. Manuka honey profiling using 1H NMR is shown to be a possible alternative to chromatography with the added advantage that it can measure methylglyoxal (MGO), dihydroxyacetone (DHA) and leptosperin simultaneously...
February 15, 2017: Food Chemistry
R White
Few involved in wound care will have escaped the considerable interest which has been generated by the resurgence in honey. Equally, there will be many clinicians around the globe who are wondering why all the fuss, as they will have been using honey all along. However, even with the advent of 'medical-grade' honey, combined with considerable research into the numerous potential modes of action, there remains a lingering scepticism regarding the value of honey as a justified, modern intervention in wound care...
September 2016: Journal of Wound Care
Joseph M Yabes, Brian K White, Clinton K Murray, Carlos J Sanchez, Katrin Mende, Miriam L Beckius, Wendy C Zera, Joseph C Wenke, Kevin S Akers
Soft-tissue invasive fungal infections are increasingly recognized as significant entities directly contributing to morbidity and mortality. They complicate clinical care, requiring aggressive surgical debridement and systemic antifungal therapy. To evaluate new topical approaches to therapy, we examined the antifungal activity and cytotoxicity of Manuka Honey (MH) and polyhexamethylene biguanide (PHMB). The activities of multiple concentrations of MH (40%, 60%, 80%) and PHMB (0.01%, 0.04%, 0.1%) against 13 clinical mould isolates were evaluated using a time-kill assay between 5 min and 24 h...
September 6, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Emmanuel Bensignor, Lionel Fabriès, Lucie Bailleux
BACKGROUND: Bacterial pyoderma is a frequent presentation in dogs. Despite the widespread availability of effective systemic and topical antimicrobial products, good clinical practice currently recommends avoidance of long-term use to mitigate the development of bacterial resistance. HYPOTHESIS/OBJECTIVES: To evaluate the speed of resolution of clinical signs of bacterial pyoderma in dogs treated with a systemic antimicrobial agent with or without the use of an adjunctive spray with antimicrobial properties...
December 2016: Veterinary Dermatology
Fabrizio Bolognese, Michela Bistoletti, Paola Barbieri, Viviana Teresa Orlandi
The antimicrobial power of honey seems to be ascribable to several factors, including oxidative and osmotic stress. The aim of this study was to find genetic determinants involved in the response to honey stress in the opportunistic pathogen Pseudomonas aeruginosa, chosen as model micro-organism. A library of transposon mutants of P. aeruginosa PAO1 was constructed and only four mutants unable to grow in presence of fir honeydew honey were selected. All four mutants were impaired in the major H2O2-scavenging enzyme catalase A (KatA)...
September 2016: Microbiology
Jessie Bong, Gordana Prijic, Terry J Braggins, Ralf C Schlothauer, Jonathan M Stephens, Kerry M Loomes
New Zealand manuka (Leptospermum scoparium) honey exhibits two unique fluorescence signatures that distinguish it from other honey types. One of these is the MM1 fluorescence marker (270-365nm excitation-emission) which we show is due to a Leptospermum nectar-derived compound, leptosperin. Synthetic or honey-purified leptosperin not only displayed an identical fluorescence spectrum, but supplementation of leptosperin into clover or artificial honeys generated the MM1 fluorescence signature. There was a quenching effect of the honey matrix on leptosperin fluorescence but otherwise leptosperin was chemically stable over prolonged storage at 37°C...
January 1, 2017: Food Chemistry
E Grego, P Robino, C Tramuta, G Giusto, M Boi, R Colombo, G Serra, S Chiadò-Cutin, M Gandini, P Nebbia
Honey as a topical treatment for infected wounds dates back to ancient times. However, few studies have been reported concerning the medical properties of Italian honey. In this study, the microbial contamination, the antimicrobial activity and the antibiotic residues of 6 different varieties of Piedmont honeys were evaluated. The antimicrobial activity of honeys was tested by agar well diffusion method and 1 honey for each variety has been selected and tested by broth micro-dilution test to determine Minimum Inhibitory Concentrations (MICs) and evaluated by Minimum Bactericidal Concentrations (MBCs)...
July 2016: Schweizer Archiv Für Tierheilkunde
Rickul Varshney, Jivianne T Lee
INTRODUCTION: Chronic rhinosinusitis (CRS) affects millions of patients worldwide. The disease is multifactorial with influences including anatomic factors, immunological disturbances, and altered sinonasal microbiome. Although oral medications are effective in controlling some symptoms, they are associated with side effects and long-term use is not ideal. Thus, topical therapies have emerged as an alternative delivery method for localized, high-concentration medication with less side effects...
August 8, 2016: Expert Opinion on Drug Delivery
Yoji Kato, Rie Fujinaka, Maki Juri, Yui Yoshiki, Akari Ishisaka, Noritoshi Kitamoto, Yoko Nitta, Hirohito Ishikawa
Syringic acid is one of the key skeletal structures of plant-derived chemicals. The derivatives of syringic acid have certain biological functions. In this study, a monoclonal antibody to syringic acid-based phytochemicals was prepared and characterized. The obtained antibody reacted with methyl syringate, syringic acid, and leonurine. Methyl syringate is a characteristic compound found in manuka honey, other honey varieties, and plants. Manuka honey was fractionated using HPLC, and the reactivity of the fractions with the antibody was examined...
August 24, 2016: Journal of Agricultural and Food Chemistry
Raman Malhotra, Kimia Ziahosseini, Cornelia Poitelea, Andre Litwin, Suresh Sagili
PURPOSE: To report outcomes of a randomized trial on the role of "active" Manuka honey on eyelid surgical wound healing. METHOD: Prospective, randomized, single-blinded study was performed for patients undergoing bilateral upper blepharoplasty. Vaseline was applied 4 times a day to both sides for 6 weeks and in addition, one eyelid was randomized to receive Manuka honey twice daily. Postoperative wounds were graded by a masked observer at 1 week, 1 month, and 4 months using Manchester scar scale and a modified eyelid scar grading scale...
July 13, 2016: Ophthalmic Plastic and Reconstructive Surgery
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