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stem cell osteoarthritis

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https://www.readbyqxmd.com/read/29146943/cryopreservation-of-human-mesenchymal-stem-cells-in-an-allogeneic-bioscaffold-based-on-platelet-rich-plasma-and-synovial-fluid
#1
Haritz Gurruchaga, Laura Saenz Del Burgo, Ane Garate, Diego Delgado, Pello Sanchez, Gorka Orive, Jesús Ciriza, Mikel Sanchez, Jose Luis Pedraz
Transplantation of mesenchymal stem cells (MSCs) has emerged as an alternative strategy to treat knee osteoarthritis. In this context, MSCs derived from synovial fluid could provide higher chondrogenic and cartilage regeneration, presenting synovial fluid as an appropriate MSCs source. An allogeneic and biomimetic bioscaffold composed of Platelet Rich Plasma and synovial fluid that preserve and mimics the natural environment of MSCs isolated from knee has also been developed. We have optimized the cryopreservation of knee-isolated MSCs embedded within the aforementioned biomimetic scaffold, in order to create a reserve of young autologous embedded knee MSCs for future clinical applications...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29143167/effectiveness-of-concurrent-procedures-during-high-tibial-osteotomy-for-medial-compartment-osteoarthritis-a-systematic-review-and-meta-analysis
#2
O-Sung Lee, Soyeon Ahn, Jin Hwan Ahn, Seow Hui Teo, Yong Seuk Lee
INTRODUCTION: The purpose of this systematic review and meta-analysis was to evaluate the efficacy of concurrent cartilage procedures during high tibial osteotomy (HTO) for medial compartment osteoarthritis (OA) by comparing the outcomes of studies that directly compared the use of HTO plus concurrent cartilage procedures versus HTO alone. MATERIALS AND METHODS: Results that are possible to be compared in more than two articles were presented as forest plots. A 95% confidence interval was calculated for each effect size, and we calculated the I (2) statistic, which presents the percentage of total variation attributable to the heterogeneity among studies...
November 15, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/29141683/pellet-coculture-of-osteoarthritic-chondrocytes-and-infrapatellar-fat-pad-derived-mesenchymal-stem-cells-with-chitosan-hyaluronic-acid-nanoparticles-promotes-chondrogenic-differentiation
#3
Shu Huang, Xiongbo Song, Tao Li, Jingfang Xiao, Yemiao Chen, Xiaoyuan Gong, Weinan Zeng, Liu Yang, Cheng Chen
BACKGROUND: Cell source plays a key role in cell-based cartilage repair and regeneration. Recent efforts in cell coculture have attempted to combine the advantages and negate the drawbacks of the constituent cell types. The aim of this study was to evaluate the chondrogenic outcome of articular chondrocytes (ACs) and infrapatellar fat pad (IPFP)-derived mesenchymal stem cells (MSCs) in direct coculture. METHODS: ACs and IPFP MSCs from the same patients with knee osteoarthritis (OA) were cocultured in monolayer and in pellets...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29134116/targeting-subchondral-bone-mesenchymal-stem-cell-activities-for-intrinsic-joint-repair-in-osteoarthritis
#4
REVIEW
Dragos C Ilas, Sarah M Churchman, Dennis McGonagle, Elena Jones
Osteoarthritis (OA) is a common age-related disease with complex pathophysiology. It is characterized by wide-ranging tissue damage and ultimate biomechanical failure of the whole joint. However, signs of tissue adaptation and attempted repair responses are evident in OA-affected osteochondral tissues. Highlighted in this review article is the role of bone-resident mesenchymal stem cells (MSCs) in these bone remodeling responses, and a proposal that targeting MSC activities in OA subchondral bone could represent a novel approach for intrinsic joint regeneration in OA...
November 2017: Future Science OA
https://www.readbyqxmd.com/read/29131526/amniotic-mesenchymal-stem-cells-mitigate-osteoarthritis-progression-in-a-synovial-macrophage-mediated-in-vitro-explant-co-culture-model
#5
Natasha Topoluk, Kathleen Steckbeck, Sandra Siatkowski, Brian Burnikel, John Tokish, Jeremy Mercuri
Osteoarthritis (OA) is a disease of the synovial joint marked by chronic, low-grade inflammation leading to cartilage destruction. Regenerative medicine strategies for mitigating OA progression and/or promoting cartilage regeneration must be assessed using models that mimic the hallmarks of OA. More specifically, these models should maintain synovial macrophage phenotype in their native micro-environment. Herein, an in vitro co-culture model of patient-matched human OA cartilage and synovium was assessed for viability, macrophage phenotype, and progressive cartilage destruction in the presence of an inflammatory milieu...
November 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29123550/mesenchymal-stem-cells-for-cartilage-regeneration-of-tmj-osteoarthritis
#6
REVIEW
Dixin Cui, Hongyu Li, Xin Xu, Ling Ye, Xuedong Zhou, Liwei Zheng, Yachuan Zhou
Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and structure-modifying effects to restore impaired cartilage as well as other TMJ tissues. In recent years, stem cell-based therapy has raised much attention as an alternative approach towards tissue repair and regeneration...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29118440/native-joint-resident-mesenchymal-stem-cells-for-cartilage-repair-in-osteoarthritis
#7
REVIEW
Dennis McGonagle, Thomas G Baboolal, Elena Jones
The role of native (not culture-expanded) joint-resident mesenchymal stem cells (MSCs) in the repair of joint damage in osteoarthritis (OA) is poorly understood. MSCs differ from bone marrow-residing haematopoietic stem cells in that they are present in multiple niches in the joint, including subchondral bone, cartilage, synovial fluid, synovium and adipose tissue. Research in experimental models suggests that the migration of MSCs adjacent to the joint cavity is crucial for chonodrogenesis during embryogenesis, and also shows that synovium-derived MSCs might be the primary drivers of cartilage repair in adulthood...
November 9, 2017: Nature Reviews. Rheumatology
https://www.readbyqxmd.com/read/29112682/cross-coupling-effects-of-silencing-of-cyclooxygenase-2-cox-2-aggrecanase-1-and-over-expressed-insulin-like-growth-factor-1-igf-1-in-an-osteoarthritis-animal-model
#8
Zhao Zhang, Xiaofei Li, Heng Huang, Guozhong Wang, Zhigang Qu, Haining Zhang
BACKGROUND This study aimed to observe the effect of lentivirus-mediated cyclooxygenase-2 and aggrecanase-1 silencing and insulin-like growth factor-1 overexpression in human bone marrow mesenchymal stem cells after injection into model osteoarthritic knees. MATERIAL AND METHODS Using genetic recombination technique, the genes of cyclooxygenase-2, aggrecanase-1, and insulin-like growth factor-1 were recombined into the lentiviral vectors, and we transfected the human bone marrow stem cells in vitro. The BMSC transfected with lentivirus without genes served as a blank-virus group, and saline was used for another control group...
November 7, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29099618/intra-articular-mesenchymal-stem-cell-therapy-for-the-human-joint-a-systematic-review
#9
James A McIntyre, Ian A Jones, Bo Han, C Thomas Vangsness
BACKGROUND: Stem cell therapy is emerging as a potential treatment of osteoarthritis (OA) and chondral defects (CDs). However, there is a great deal of heterogeneity in the literature. The indications for stem cell use, the ideal tissue source, and the preferred outcome measures for stem cell-based treatments have yet to be determined. PURPOSE: To provide clinicians with a comprehensive overview of the entire body of the current human literature investigating the safety and efficacy of intra-articular mesenchymal stem cell (MSC) therapy in all joints...
November 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29083347/current-concepts-in-treatment-of-early-knee-osteoarthritis-and-osteochondral-lesions-the-role-of-biological-augmentations
#10
C Dall'Oca, M Cengarle, A Costanzo, N Giannini, A Vacchiano, B Magnan
Early knee osteoarthritis and chondral lesions are a common cause of disability in younger patients. Surgical options, such as microfractures, ACI and OAT, provide good, but not fully satisfying, outcomes. Recent advance in biological knowledge introduced two different methodological approaches of delivering growth factors and stem cells into the articular environment. Platelet-Rich Plasma and Mesenchimal Stem Cells are supposed to change the way to approach early knee osteoarthritis and chondral lesions, though their indications and limits are yet to be determined...
October 18, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/29062653/ascot-autologous-bone-marrow-stem-cell-use-for-osteoarthritis-of-the-thumb-first-carpometacarpal-joint
#11
Matthew P Murphy, Christina Buckley, Conor Sugrue, Emma Carr, Aine O'Reilly, Shane O'Neill, Sean M Carroll
BACKGROUND: The first carpometacarpal joint (CMCJ) in the hand is a commonly affected joint by osteoarthritis. It causes significant thumb base pain, limiting functional capacity. Microfracturing and application of autologous stem cells has been performed on large joints such as the knee but has never been evaluated for use in the smaller joints in the hand. Our aim was to determine the potential benefit of microfracturing and autologous bone marrow stem cells for treatment of osteoarthritis of the first CMCJ in the hand...
September 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29062478/mesenchymal-stem-cells-in-human-meniscal-regeneration-a-systematic-review
#12
REVIEW
Ernest Chew, Rohan Prakash, Wasim Khan
BACKGROUND: Stem cell regeneration is the holy grail of meniscal tissue repair. Currently, the best treatment is to preserve the original meniscus but if it fails, a partial meniscectomy is indicated to delay the onset of osteoarthritis. MATERIALS AND METHODS: The authors present a systematic review to determine the up-to-date evidence underlying the use of mesenchymal stem cells for meniscal regeneration in humans. A search was conducted using the electronic databases of MEDLINE/Pubmed, Google scholar, and the Cochrane Collaboration...
December 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/29056974/avoidance-of-total-knee-arthroplasty-in-early-osteoarthritis-of-the-knee-with-intra-articular-implantation-of-autologous-activated-peripheral-blood-stem-cells-versus-hyaluronic-acid-a-randomized-controlled-trial-with-differential-effects-of-growth-factor-addition
#13
Thana Turajane, Ukrit Chaveewanakorn, Warachaya Fongsarun, Jongjate Aojanepong, Konstantinos I Papadopoulos
In this randomized controlled trial, in early osteoarthritis (OA) that failed conservative intervention, the need for total knee arthroplasty (TKA) and WOMAC scores were evaluated, following a combination of arthroscopic microdrilling mesenchymal cell stimulation (MCS) and repeated intra-articular (IA) autologous activated peripheral blood stem cells (AAPBSCs) with growth factor addition (GFA) and hyaluronic acid (HA) versus IA-HA alone. Leukapheresis-harvested AAPBSCs were administered as three weekly IA injections combined with HA and GFA (platelet-rich plasma [PRP] and granulocyte colony-stimulating factor [hG-CSF]) and MCS in group 1 and in group 2 but without hG-CSF while group 3 received IA-HA alone...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29053693/in-vivo-tracking-of-human-adipose-derived-mesenchymal-stem-cells-in-a-rat-knee-osteoarthritis-model-with-fluorescent-lipophilic-membrane-dye
#14
Meng Li, Ming Hao, Dong Jiang, Yanxi Chen, Wen Wang
In order to support the clinical application of human adipose-derived mesenchymal stem cell (haMSC) therapy for knee osteoarthritis (KOA), we examined the efficacy of cell persistence and biodistribution of haMSCs in animal models. We demonstrated a method to label the cell membrane of haMSCs with lipophilic fluorescent dye. Subsequently, intra-articular injection of the labeled cells in rats with surgically induced KOA was monitored dynamically by an in vivo imaging system. We employed the lipophilic carbocyanines DiD (DilC18 (5)), a far-red fluorescent Dil (dialkylcarbocyanines) analog, which utilized a red laser to avoid excitation of the natural green autofluorescence from surrounding tissues...
October 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29050153/-study-on-the-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells-into-chondrocytes-induced-by-tgf-beta-3
#15
D B Liu, X S Han, W L Huang, J Deng, R F She
Objective: To explore the methods for the differentiation of rat bone marrow mesenchymal stem cells (BMMSCs) into chondrocytes induced by transforming growth factor (TGF)-beta 3 in vitro, and search for a reliable seed cell for the regeneration and repair of articular cartilage in osteoarthritis. Methods: SD rat femoral and tibial BMMSCs were cultured by the whole bone marrow adherent method, and then the purity was identified by flow cytometry. P3 generation cells were induced and differentiated into chondrocytes by the induction of differentiation medium containing TGF-beta 3, and cell chondrogenic differentiation ability at different induction time points was detected...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29038440/mir-29b-regulates-expression-of-collagens-i-and-iii-in-chondrogenically-differentiating-bmsc-in-an-osteoarthritic-environment
#16
Ute Mayer, Achim Benditz, Susanne Grässel
Osteoarthritis (OA) is characterized by a slowly progressing, irreversible loss of articular cartilage. Tissue engineering approaches for cartilage regeneration include stem cell-based strategies but not much is known about their repair capacity in an OA microenvironment. The aim of the present study was to identify factors regulating collagen expression during chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSC) in an OA microenvironment. Coculture with OA cartilage induced miR-29b expression in BMSC which inhibited collagen I and III expression...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038190/effect-of-autologous-adipose-derived-mesenchymal-stem-cell-therapy-in-the-treatment-of-a-post-traumatic-chondral-defect-of-the-knee
#17
Julien Freitag, Douglas Li, James Wickham, Kiran Shah, Abi Tenen
Isolated chondral defects have a limited capacity to heal and predispose to the development of osteoarthritis. Current surgical management can be unpredictable in outcome. Improved understanding of the action of mesenchymal stem cells (MSCs) has seen renewed interest in their role in cartilage repair. A 26-year-old athlete presented with a post-traumatic, isolated patella chondral defect. The patient underwent an arthroscopy with removal of a chondral loose body. After failure to symptomatically improve 12 months following surgery, the patient received intra-articular autologous adipose-derived mesenchymal stem cell (ADMSC) therapy...
October 15, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29035932/extracellular-vesicles-in-cartilage-homeostasis-and-osteoarthritis
#18
Shigeru Miyaki, Martin K Lotz
PURPOSE OF REVIEW: Extracellular vesicles carry bioactive molecules that can be transferred between cells and tissues. The purpose of this review is to describe how extracellular vesicles regulate functions of cells in cartilage and other joint tissues. The potential application of extracellular vesicles in the treatment of osteoarthritis and as biomarkers will also be discussed. RECENT FINDINGS: Extracellular vesicles are found in synovial fluid, in articular cartilage and in the supernatants of synoviocytes and chondrocytes...
October 13, 2017: Current Opinion in Rheumatology
https://www.readbyqxmd.com/read/29035380/mixed-cell-therapy-of-bone-marrow-derived-mesenchymal-stem-cells-and-articular-cartilage-chondrocytes-ameliorates-osteoarthritis-development
#19
Indira Prasadam, Akoy Akuien, Thor E Friis, Wei Fang, Xinzhan Mao, Ross W Crawford, Yin Xiao
Of the many cell-based treatments that have been tested in an effort to regenerate osteoarthritic articular cartilage, none have ever produced cartilage that compare with native hyaline cartilage. Studies show that different cell types lead to inconsistent results and for cartilage regeneration to be considered successful, there must be an absence of fibrotic tissue. Here we report of a series of experiments in which bone marrow-derived stem cells (BMSCs) and articular cartilage chondrocytes (ACCs) were mixed in a 1:1 ratio and tested for their ability to enhance cartilage regeneration in three different conditions: (1) in an in vitro differentiation model; (2) in an ex vivo cartilage defect model implanted subcutaneously in mice; and (3) as an intra-articular injection in a meniscectomy-induced OA model in rats...
October 16, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29031987/intra-articular-injection-of-mesenchymal-stem-cells-and-platelet-rich-plasma-to-treat-patellofemoral-osteoarthritis-preliminary-results-of-a-long-term-pilot-study
#20
Julien Pintat, Alain Silvestre, Guy Magalon, Alain Pierre Gadeau, Lionel Pesquer, Anne Perozziello, Alain Peuchant, Charbel Mounayer, Benjamin Dallaudière
PURPOSE: To assess the feasibility and safety of concomitant intra-articular (IA) knee injection of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) under fluoroscopic guidance to treat patellofemoral osteoarthritis (OA). MATERIALS AND METHODS: This prospective study included 19 consecutive patients referred for fluoroscopically guided IA MSC and PRP injection for symptomatic patellofemoral chondropathy in which conservative treatment had failed. Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score and magnetic resonance (MR) data, including T2 mapping sequence, were prospectively collected before and 6 months after treatment...
October 12, 2017: Journal of Vascular and Interventional Radiology: JVIR
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