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https://www.readbyqxmd.com/read/28606503/optimizing-preoperative-expectations-leads-to-a-shorter-length-of-hospital-stay-in-cabg-patients-further-results-of-the-randomized-controlled-psy-heart-trial
#1
Charlotte J Auer, Johannes A C Laferton, Meike C Shedden-Mora, Stefan Salzmann, Rainer Moosdorf, Winfried Rief
OBJECTIVE: To examine the effect of a preoperative expectation-optimizing psychological intervention on length of stay in the hospital and time spent in the Intensive Care Unit (ICU) in patients undergoing elective cardiac surgery. METHODS: In a randomized controlled trial, 124 patients prior to undergoing coronary artery bypass grafting (CABG) or CABG combined with heart valve surgery were randomized to either a) standard medical care alone (SMC) or an additional preoperative intervention, b) an additional expectation manipulation intervention (EXPECT) to optimize patients' expectations, or c) an additional supportive therapy (SUPPORT), containing the same amount of therapeutic attention but without a specific focus...
June 2017: Journal of Psychosomatic Research
https://www.readbyqxmd.com/read/28600193/functional-and-molecular-responses-of-the-blue-mussel-mytilus-edulis-hemocytes-exposed-to-cadmium-an-in-vitro-model-and-transcriptomic-approach
#2
Philippine Granger Joly de Boissel, Michel Fournier, Juan Carlos Rodriguez-Lecompte, Patty McKenna, Frederick Kibenge, Ahmed Siah
The bivalve mollusk, Mytilus edulis, is used as a sentinel species in several monitoring programs due to its ability to bio-accumulate contaminants. Its immune system consists of hemocytes and humoral components, which constitute the main part of the hemolymph. The present study is aimed at understanding the effects of Cd on the differentially expressed genes involved in the phagocytosis of M. edulis' hemocytes. Our approach focuses on an in vitro model by exposing hemocytes to different concentrations of Cd ranging from 10(-9) M to 10(-3) M...
June 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28581523/a-dna-methylation-map-of-human-cancer-at-single-base-pair-resolution
#3
E Vidal, S Sayols, S Moran, A Guillaumet-Adkins, M P Schroeder, R Royo, M Orozco, M Gut, I Gut, N Lopez-Bigas, H Heyn, M Esteller
Although single base-pair resolution DNA methylation landscapes for embryonic and different somatic cell types provided important insights into epigenetic dynamics and cell-type specificity, such comprehensive profiling is incomplete across human cancer types. This prompted us to perform genome-wide DNA methylation profiling of 22 samples derived from normal tissues and associated neoplasms, including primary tumors and cancer cell lines. Unlike their invariant normal counterparts, cancer samples exhibited highly variable CpG methylation levels in a large proportion of the genome, involving progressive changes during tumor evolution...
June 5, 2017: Oncogene
https://www.readbyqxmd.com/read/28564635/a-high-plasma-d-dimer-level-predicts-poor-prognosis-in-gynecological-tumors-in-east-asia-area-a-systematic-review-and-meta-analysis
#4
Lei Xu, Fan He, Hongcai Wang, Bei Gao, Huini Wu, Shuping Zhao
High pre-treatment plasma D-dimer levels have been reported as a factor associated with a poor prognosis in different types of malignancies, including pancreatic, gastric, colorectal, lung, and nasopharyngeal carcinoma. Here, we performed a meta-analysis to determine the association of plasma D-dimer levels and long term survival in gynecological cancers, including ovarian, cervical and endometrial carcinoma. We searched all eligible publications in PubMed and Web of Science Databases up to August 2016. Primary outcomes, including overall survival (OS), disease-free survival and hazard ratios (HR) of were extracted and analyzed...
May 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28561580/free-energy-calculations-of-ionic-hydration-consistent-with-the-experimental-hydration-free-energy-of-the-proton
#5
Haiyang Zhang, Yang Jiang, Hai Yan, Chunhua Yin, Tianwei Tan, David van der Spoel
Computational free-energy correction strategies and the choice of experimental proton hydration free energy, ΔGs(*)(H(+)), are analyzed to investigate the apparent controversy in experimental thermodynamics of ionic hydration. Without corrections, the hydration free-energy (ΔGhyd) calculations match experiments with ΔGs(*)(H(+)) = -1064 kJ/mol as reference. Using the Galvani surface potential the resulting (real) ΔGhyd are consistent with ΔGs(*)(H(+)) = -1098 kJ/mol. When applying, in an ad hoc manner, the discrete solvent correction, ΔGhyd matching the "consensus" ΔGs(*)(H(+)) of -1112 kJ/mol are obtained...
June 15, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28557557/subsequent-surgery-after-revision-anterior-cruciate-ligament-reconstruction-rates-and-risk-factors-from-a-multicenter-cohort
#6
David Y Ding, Alan L Zhang, Christina R Allen, Allen F Anderson, Daniel E Cooper, Thomas M DeBerardino, Warren R Dunn, Amanda K Haas, Laura J Huston, Brett Brick A Lantz, Barton Mann, Kurt P Spindler, Michael J Stuart, Rick W Wright, John P Albright, Annunziato Ned Amendola, Jack T Andrish, Christopher C Annunziata, Robert A Arciero, Bernard R Bach, Champ L Baker, Arthur R Bartolozzi, Keith M Baumgarten, Jeffery R Bechler, Jeffrey H Berg, Geoffrey A Bernas, Stephen F Brockmeier, Robert H Brophy, Charles A Bush-Joseph, J Brad Butler, John D Campbell, James L Carey, James E Carpenter, Brian J Cole, Jonathan M Cooper, Charles L Cox, R Alexander Creighton, Diane L Dahm, Tal S David, David C Flanigan, Robert W Frederick, Theodore J Ganley, Elizabeth A Garofoli, Charles J Gatt, Steven R Gecha, James Robert Giffin, Sharon L Hame, Jo A Hannafin, Christopher D Harner, Norman Lindsay Harris, Keith S Hechtman, Elliott B Hershman, Rudolf G Hoellrich, Timothy M Hosea, David C Johnson, Timothy S Johnson, Morgan H Jones, Christopher C Kaeding, Ganesh V Kamath, Thomas E Klootwyk, Bruce A Levy, C Benjamin Ma, G Peter Maiers, Robert G Marx, Matthew J Matava, Gregory M Mathien, David R McAllister, Eric C McCarty, Robert G McCormack, Bruce S Miller, Carl W Nissen, Daniel F O'Neill, Brett D Owens, Richard D Parker, Mark L Purnell, Arun J Ramappa, Michael A Rauh, Arthur C Rettig, Jon K Sekiya, Kevin G Shea, Orrin H Sherman, James R Slauterbeck, Matthew V Smith, Jeffrey T Spang, Steven J Svoboda, Timothy N Taft, Joachim J Tenuta, Edwin M Tingstad, Armando F Vidal, Darius G Viskontas, Richard A White, James S Williams, Michelle L Wolcott, Brian R Wolf, James J York
BACKGROUND: While revision anterior cruciate ligament reconstruction (ACLR) can be performed to restore knee stability and improve patient activity levels, outcomes after this surgery are reported to be inferior to those after primary ACLR. Further reoperations after revision ACLR can have an even more profound effect on patient satisfaction and outcomes. However, there is a current lack of information regarding the rate and risk factors for subsequent surgery after revision ACLR. PURPOSE: To report the rate of reoperations, procedures performed, and risk factors for a reoperation 2 years after revision ACLR...
May 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28551732/bradyrhizobium-brasilense-sp-nov-a-symbiotic-nitrogen-fixing-bacterium-isolated-from-brazilian-tropical-soils
#7
Elaine Martins da Costa, Amanda Azarias Guimarães, Rayssa Pereira Vicentin, Paula Rose de Almeida Ribeiro, Aniele Carolina Ribas Leão, Eduardo Balsanelli, Liesbeth Lebbe, Maarten Aerts, Anne Willems, Fatima Maria de Souza Moreira
Four strains of rhizobia isolated from nodules of Vigna unguiculata (UFLA03-321(T), UFLA03-320 and UFLA03-290) and Macroptilium atropurpureum (UFLA04-0212) in Brazilian soils were previously reported as a new group within the genus Bradyrhizobium. To determine their taxonomic position, these strains were characterized in this study using a polyphasic approach. The analysis of the 16S rRNA gene grouped the four strains with Bradyrhizobium pachyrhizi PAC48(T). However, the concatenated sequence analysis of the two (recA and glnII) or three (atpD, gyrB and recA) housekeeping genes indicated that these strains represent a novel species of Bradyrhizobium, which is very closely related to B...
May 27, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28505290/supraphysiologic-estradiol-is-an-independent-predictor-of-low-birth-weight-in-full-term-singletons-born-after-fresh-embryo-transfer
#8
N Pereira, R T Elias, P J Christos, A C Petrini, K Hancock, J P Lekovich, Z Rosenwaks
STUDY QUESTION: Is supraphysiologic estradiol (E2) an independent predictor of low birth weight (LBW) in singletons born after fresh IVF-embryo transfer (ET) cycles? SUMMARY ANSWER: Our results suggest that E2 > 2500 pg/ml is an independent predictor for LBW in full-term singletons born to normal responder patients undergoing fresh IVF-ET cycles. WHAT IS KNOWN ALREADY: The pathogenesis of LBW in IVF singletons remains unknown. However, recent studies have suggested that the hyperestrogenic milieu generated during ovarian stimulation may create a sub-optimal peri-implantation environment, leading to placental dysfunction, and therefore, LBW...
May 13, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28497564/no-significant-enrichment-of-rare-functionally-defective-cpa1-variants-in-a-large-chinese-idiopathic-chronic-pancreatitis-cohort
#9
Hao Wu, Dai-Zhan Zhou, Dorottya Berki, Andrea Geisz, Wen-Bin Zou, Xiao-Tian Sun, Liang-Hao Hu, Zhen-Hua Zhao, An-Jing Zhao, Lin He, David N Cooper, Claude Férec, Jian-Min Chen, Zhao-Shen Li, Miklós Sahin-Tóth, Zhuan Liao
Rare functionally defective carboxypeptidase A1 (CPA1) variants have been reported to predispose to nonalcoholic chronic pancreatitis, mainly the idiopathic subtype. However, independent replication has so far been lacking, particularly in Asian cohorts where initial studies employed small sample sizes. Herein we performed targeted next-generation sequencing of the CPA1 gene in 1112 Han Chinese idiopathic chronic pancreatitis (ICP) patients - the largest ICP cohort so far analyzed in a single population - and 1580 controls...
May 12, 2017: Human Mutation
https://www.readbyqxmd.com/read/28456510/high-acuity-sleeve-gastrectomy-patients-in-a-free-standing-ambulatory-surgical-center
#10
Peter Billing, Josiah Billing, Jedediah Kaufman, Kurt Stewart, Eric Harris, Rob Landerholm
BACKGROUND: Procedures performed in ambulatory surgical centers (ASC) can provide several advantages over hospital-based surgery. Understandably, concerns have been raised regarding "high acuity" cases in the ASC setting. Recently the Metabolic and Bariatric Surgery Accreditation and Quality Improvement Program (MBSAQIP) presented protocols for ASCs to follow, requiring them to perform only "low acuity" cases to be compliant with accreditation. OBJECTIVE: Assess the safety and efficacy of outpatient sleeve gastrectomy (SG) on the "high acuity patient" in a free-standing ASC...
March 27, 2017: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28450139/3d-fully-convolutional-networks-for-subcortical-segmentation-in-mri-a-large-scale-study
#11
REVIEW
Jose Dolz, Christian Desrosiers, Ismail Ben Ayed
This study investigates a 3D and fully convolutional neural network (CNN) for subcortical brain structure segmentation in MRI. 3D CNN architectures have been generally avoided due to their computational and memory requirements during inference. We address the problem via small kernels, allowing deeper architectures. We further model both local and global context by embedding intermediate-layer outputs in the final prediction, which encourages consistency between features extracted at different scales and embeds fine-grained information directly in the segmentation process...
April 24, 2017: NeuroImage
https://www.readbyqxmd.com/read/28425233/comparison-of-the-standard-gnrh-antagonist-protocol-and-the-luteal-phase-estradiol-gnrh-antagonist-priming-protocol-in-poor-ovarian-responders
#12
Mehmet Fırat Mutlu, İlknur Mutlu, Mehmet Erdem, İsmail Güler, Ahmet Erdem
BACKGROUND/AIM: The aim of the study was to compare the luteal estradiol patch/GnRH antagonists priming protocol (LPP) with the standard GnRH antagonist protocol in poor ovarian responders (PORs) in terms of the outcomes of in vitro fertilization (IVF) treatment. MATERIALS AND METHODS: IVF outcomes of 265 cycles in 265 patients (106 in the LPP group, 159 in the standard GnRH antagonist group) were evaluated retrospectively. RESULTS: Mean length of stimulation (11...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28407120/biological-versus-mechanical-bentall-procedure-for-aortic-root-replacement-a-propensity-score-analysis-of-a-consecutive-series-of-1112-patients%C3%A2
#13
Antonio Pantaleo, Giacomo Murana, Luca Di Marco, Giuliano Jafrancesco, Giuseppe Barberio, Paolo Berretta, Alessandro Leone, Roberto Di Bartolomeo, Davide Pacini
OBJECTIVES: In this study, a propensity-matching analysis was used to compare biological versus mechanical composite valve graft implantation for early mortality and morbidities and for late complications including the need for aortic reintervention. METHODS: Between 1978 and 2011, 1112 consecutive patients underwent a complete aortic root replacement using either a biological Bentall (BB, n  = 356) or a mechanical Bentall (MB, n  = 756) valve conduit. Preoperative data were stratified according to the type of valve graft, and treatment bias was addressed by propensity score analysis...
April 11, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28396619/the-complete-mitogenome-of-orcula-dolium-draparnaud-1801-ultra-deep-sequencing-from-a-single-long-range-pcr-using-the-ion-torrent-pgm
#14
D S J Groenenberg, J Harl, E Duijm, E Gittenberger
BACKGROUND: With the increasing capacity of present-day next-generation sequencers the field of mitogenomics is rapidly changing. Enrichment of the mitochondrial fraction, is no longer necessary for obtaining mitogenomic data. Despite the benefits, shotgun sequencing approaches also have disadvantages. They do not guarantee obtaining the complete mitogenome, generally require larger amounts of input DNA and coverage is low compared to sequencing with enrichment strategies. If the mitogenome could be amplified in a single amplification, additional time and costs for sample preparation might outweigh these disadvantages...
2017: Hereditas
https://www.readbyqxmd.com/read/28386748/open-and-endovascular-elective-treatment-of-abdominal-aortic-aneurysms-a-real-world-experience
#15
Daniela Mazzaccaro, Giovanni Nano, Alberto M Settembrini, Michele Carmo, Raffaello Dallatana, Simone Salvati, Giovanni Malacrida, Piergiorgio G Settembrini
PURPOSE: To present a real-world experience of the elective treatment of abdominal aortic aneurysms (AAAs) using both open repair (OR) and endovascular repair (EVAR). METHODS: Data from patients treated consecutively between January 1, 2000 and December 31, 2014 were collected retrospectively and reviewed. The primary outcomes were 30-day mortality and complication rates, freedom from reintervention, and survival in the long-term. RESULTS: We analyzed data on 1112 patients (660 EVAR, 452 OR)...
April 6, 2017: Surgery Today
https://www.readbyqxmd.com/read/28385120/are-routine-preoperative-liver-function-tests-in-patients-with-uncomplicated-symptomatic-gallstone-disease-necessary
#16
Marijke van den Berg, Gijs M van Couwelaar, Christel A L de Raaff, Sjoerd M Lagarde, Pieter Joosse, Bart A van Wagensveld, Bart C Vrouenraets
BACKGROUND: The aim of this study was to evaluate the value of preoperative liver function tests (LFTs) in patients with uncomplicated gallstone disease and scheduled for laparoscopic cholecystectomy. METHODS: All 1112 patients who underwent a laparoscopic cholecystectomy for symptomatic gallstone disease during a 6-year cohort were retrospectively reviewed. Only patients who presented with uncomplicated disease were selected. Preoperative LFTs, pre-, and postoperative endoscopic retrograde cholangio pancreaticographies (ERCPs) and postoperative complications were collected...
April 7, 2017: Acta Chirurgica Belgica
https://www.readbyqxmd.com/read/28368326/mineral-and-anti-nutritional-contents-of-niger-seed-guizotia-abyssinica-l-f-cass-linseed-linumusitatissimum-l-and-sesame-sesamumindicum-l-varieties-grown-in-ethiopia
#17
Tesfaye Deme, Gulelat D Haki, Nigussie Retta, Ashagrie Woldegiorgis, Mulatu Geleta
Oilseeds are rich sources of micronutrients and contribute to combating malnutrition caused by micronutrient deficiency. The objective of this study was to investigate the mineral and anti-nutritional contents of different varieties of niger seed, linseed and sesame. Five niger seed, eight linseed and ten sesame varieties were used. Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) was used for mineral analysis and the standard method was adopted to estimate tannin and phytate. Twelve mineral elements; Ca, K, Mg, Na, P, B, Cu, Fe, Mn, S, Se and Zn were analyzed for each oilseed variety...
April 1, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28361913/multi-scale-radiomic-analysis-of-sub-cortical-regions-in-mri-related-to-autism-gender-and-age
#18
Ahmad Chaddad, Christian Desrosiers, Matthew Toews
We propose using multi-scale image textures to investigate links between neuroanatomical regions and clinical variables in MRI. Texture features are derived at multiple scales of resolution based on the Laplacian-of-Gaussian (LoG) filter. Three quantifier functions (Average, Standard Deviation and Entropy) are used to summarize texture statistics within standard, automatically segmented neuroanatomical regions. Significance tests are performed to identify regional texture differences between ASD vs. TDC and male vs...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28339460/evaluating-implementation-of-a-fire-prevention-injury-prevention-briefing-in-children-s-centres-cluster-randomised-controlled-trial
#19
Toity Deave, Adrian Hawkins, Arun Kumar, Mike Hayes, Nicola Cooper, Michael Watson, Joanne Ablewhite, Carol Coupland, Alex Sutton, Gosia Majsak-Newman, Lisa McDaid, Trudy Goodenough, Kate Beckett, Elaine McColl, Richard Reading, Denise Kendrick
BACKGROUND: Many developed countries have high mortality rates for fire-related deaths in children aged 0-14 years with steep social gradients. Evidence-based interventions to promote fire safety practices exist, but the impact of implementing a range of these interventions in children's services has not been assessed. We developed an Injury Prevention Briefing (IPB), which brought together evidence about effective fire safety interventions and good practice in delivering interventions; plus training and facilitation to support its use and evaluated its implementation...
2017: PloS One
https://www.readbyqxmd.com/read/28327424/cellulase-assisted-extraction-of-polysaccharides-from-malva-sylvestris-process-optimization-and-potential-functionalities
#20
Hosein Rostami, Seyed Mohammad Taghi Gharibzahedi
Enzyme-assisted extraction process of the water-soluble Malva sylvestris polysaccharides (MSPs) was optimized using response surface methodology (RSM). The highest yield (10.40%) of MSPs was achieved at 5.64% cellulase, 55.65°C temperature, 3.4h time, and 5.22 pH. Three homogeneous polysaccharide fractions (MSP-1, MSP-2, MSP-3) were purified by DEAE-cellulose and Sephadex G-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar ratios with molecular weight range of 2...
August 2017: International Journal of Biological Macromolecules
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