keyword
MENU ▼
Read by QxMD icon Read
search

Flow cytometry antibiotic

keyword
https://www.readbyqxmd.com/read/27878810/improvement-of-phytoplankton-culture-isolation-using-single-cell-sorting-by-flow-cytometry
#1
Dominique Marie, Florence Le Gall, Roseline Edern, Priscillia Gourvil, Daniel Vaulot
Flow cytometry provides a tool to physically sort single algal cells in order to obtain clonal cultures. During sorting, cells are submitted to physical stress factors such as high fluidic pressure, exposure to the laser beam, electrostatic charges, deflection through high voltage fields and collisions with container surfaces. All of these can damage the cells of interest and success rates for initiation of cultures from flow-sorted cells are generally very low. We found that the addition of Bovine Serum Albumin (BSA) in the culture medium into which cells are sorted drastically improved the success of initiation of pico- and nano-eukaryotic phytoplankton strains...
November 22, 2016: Journal of Phycology
https://www.readbyqxmd.com/read/27877159/efficacy-of-antibacterial-peptides-against-peptide-resistant-mrsa-is-restored-by-permeabilization-of-bacteria-membranes
#2
Joshua Ravensdale, Zachary Wong, Frances O'Brien, Keith Gregg
Clinical application of antimicrobial peptides (AMPs), as with conventional antibiotics, may be compromised by the development of bacterial resistance. This study investigated AMP resistance in methicillin resistant Staphylococcus aureus, including aspects related to the resilience of the resistant bacteria toward the peptides, the stability of resistance when selection pressures are removed, and whether resistance can be overcome by using the peptides with other membrane-permeabilising agents. Genotypically variant strains of S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27867043/acute-social-stress-induced-immunomodulation-in-pigs-high-and-low-responders-to-acth
#3
Elodie Bacou, Karine Haurogné, Grégoire Mignot, Marie Allard, Laurence De Beaurepaire, Jordan Marchand, Elena Terenina, Yvon Billon, Julien Jacques, Jean-Marie Bach, Pierre Mormède, Julie Hervé, Blandine Lieubeau
Pig husbandry is known as an intensive breeding system, piglets being submitted to multiple stressful events such as early weaning, successive mixing, crowding and shipping. These stressors are thought to impair immune defences and might contribute, at least partly, to the prophylactic use of antibiotics. Robustness was recently defined as the ability of an individual to express a high-production potential in a wide variety of environmental conditions. Increasing robustness thus appears as a valuable option to improve resilience to stressors and could be obtained by selecting piglets upon their adrenocortical activity...
November 17, 2016: Physiology & Behavior
https://www.readbyqxmd.com/read/27856622/in-vitro-fermentation-of-b-gos-impact-on-faecal-bacterial-populations-and-metabolic-activity-in-autistic-and-non-autistic-children
#4
Roberta Grimaldi, Drinalda Cela, Jonathan R Swann, Jelena Vulevic, Glenn R Gibson, George Tzortzis, Adele Costabile
Children with autism spectrum disorders (ASD) often suffer gastrointestinal problems consistent with imbalances in the gut microbial population. Treatment with antibiotics or pro/prebiotics has been postulated to regulate microbiota and improve gut symptoms, but there is a lack of evidence for such approaches, especially for prebiotics. This study assessed the influence of a prebiotic galactooligosaccharide (B-GOS) on gut microbial ecology and metabolic function using faecal samples from autistic and non-autistic children in an in vitro gut model system...
November 16, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27855654/salinomycin-inhibits-metastatic-colorectal-cancer-growth-and-interferes-with-wnt-%C3%AE-catenin-signaling-in-cd133-human-colorectal-cancer-cells
#5
Johannes Klose, Jana Eissele, Claudia Volz, Steffen Schmitt, Alina Ritter, Shen Ying, Thomas Schmidt, Ulrike Heger, Martin Schneider, Alexis Ulrich
BACKGROUND: The polyether antibiotic Salinomycin (Sal) is regarded as an inhibitor of cancer stem cells. Its effectiveness on human colorectal cancer (CRC) cells in vitro has been demonstrated before. The aim of this study was to establish a murine model to investigate the effectiveness of Sal in vivo. Furthermore, we investigated the impact of Sal on Wnt/β-catenin signaling in human CD133(+) CRC cells. METHODS: The two murine CRC cell lines MC38 and CT26 were used to analyze the impact of Sal on tumor cell proliferation, viability, migration, cell cycle progression and cell death in vitro...
November 17, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27803171/the-early-adaptive-immune-response-in-the-pathophysiological-process-of-pneumococcal-meningitis
#6
Sandra Ribes, Stefan Nessler, Ev-Christin Heide, Dörthe Malzahn, Christina Perske, Wolfgang Brück, Roland Nau
BACKGROUND:  The adaptive immune system has been considered to play a minimal role in the early host response during bacterial meningitis. METHODS:  We investigated the progression and outcome of pneumococcal meningitis in Rag1(-/-) mice lacking functional B and T cells by assessing overall and symptom-free survival, bacteriological and histological studies, as well as flow cytometry and measurements of proinflammatory mediators. RESULTS:  The intracerebral injection of S...
October 31, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27795387/dual-reporter-mycobacteriophages-%C3%AE-2drms-reveal-preexisting-mycobacterium-tuberculosis-persistent-cells-in-human-sputum
#7
Paras Jain, Brian C Weinrick, Eric J Kalivoda, Hui Yang, Vanisha Munsamy, Catherine Vilcheze, Torin R Weisbrod, Michelle H Larsen, Max R O'Donnell, Alexander Pym, William R Jacobs
: Persisters are the minor subpopulation of bacterial cells that lack alleles conferring resistance to a specific bactericidal antibiotic but can survive otherwise lethal concentrations of that antibiotic. In infections with Mycobacterium tuberculosis, such persisters underlie the need for long-term antibiotic therapy and contribute to treatment failure in tuberculosis cases. Here, we demonstrate the value of dual-reporter mycobacteriophages (Φ(2)DRMs) for characterizing M. tuberculosis persisters...
October 25, 2016: MBio
https://www.readbyqxmd.com/read/27780954/antimicrobial-activity-of-the-scolopendrasin-v-peptide-identified-from-the-centipede-scolopendra-subspinipes-mutilans
#8
Joon Ha Lee, In-Woo Kim, Mi-Ae Kim, Mi-Young Ahn, Eun-Young Yun, Jae Sam Hwang
In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next generation sequencing technology and identified several antimicrobial peptide candidates. One of peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V by using radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry...
October 25, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27776487/the-cox-inhibitor-indomethacin-reduces-th1-effector-and-t-regulatory-cells-in-vitro-in-mycobacterium-tuberculosis-infection
#9
Kristian Tonby, Ida Wergeland, Nora V Lieske, Dag Kvale, Kjetil Tasken, Anne M Dyrhol-Riise
BACKGROUND: Tuberculosis (TB) causes a major burden on global health with long and cumbersome TB treatment regimens. Host-directed immune modulating therapies have been suggested as adjunctive treatment to TB antibiotics. Upregulated cyclooxygenase-2 (COX-2)-prostaglandin E2 (PGE2) signaling pathway may cause a dysfunctional immune response that favors survival and replication of Mycobacterium tuberculosis (Mtb). METHODS: Blood samples were obtained from patients with latent TB (n = 9) and active TB (n = 33) before initiation of anti-TB chemotherapy...
October 24, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27775322/inactivation-of-antibiotic-resistant-bacteria-and-resistance-genes-by-ozone-from-laboratory-experiments-to-full-scale-wastewater-treatment
#10
Nadine Czekalski, Stefanie Imminger, Elisabeth Salhi, Marjan Veljkovic, Karolin Kleffel, David Drissner, Frederik Hammes, Helmut Bürgmann, Urs von Gunten
Ozone, a strong oxidant and disinfectant, seems ideal to cope with future challenges of water treatment, such as micropollutants, multiresistant bacteria (MRB) and even intracellular antibiotic resistance genes (ARG), but information on the latter is scarce. In ozonation experiments we simultaneously determined kinetics and dose-dependent inactivation of Escherichia coli and its plasmid-encoded sulfonamide resistance gene sul1 in different water matrixes. Effects in E. coli were compared to an autochthonous wastewater community...
November 1, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27743797/enhanced-plasmid-loss-in-bacterial-populations-exposed-to-the-antimicrobial-compound-irgasan-delivered-from-interpenetrating-polymer-network-silicone-hydrogels
#11
Leise Riber, Mette Burmølle, Martin Alm, Stefan M Milani, Peter Thomsen, Lars H Hansen, Søren J Sørensen
The spread of antimicrobial resistance, usually mediated by horizontal transfer of plasmids, limits the options of treating bacterial infections and thereby poses a crucial human health problem. The disturbance of plasmid stability within bacterial species in clinical environments serves as a novel strategy to reduce the development and dissemination of antibiotic resistance. We tested the ability of irgasan to destabilize plasmids from Escherichia coli K-12 cells when added directly into liquid growth medium at concentrations below levels of marked bacterial growth inhibition, or when released into liquid growth medium from irgasan-impregnated Interpenetrating Polymer Network (IPN) silicone hydrogel objects, a novel technology developed as drug-delivery platform...
October 12, 2016: Plasmid
https://www.readbyqxmd.com/read/27737551/fluorescent-trimethoprim-conjugate-probes-to-assess-drug-accumulation-in-wild-type-and-mutant-escherichia-coli
#12
Wanida Phetsang, Ruby Pelingon, Mark S Butler, Sanjaya Kc, Miranda E Pitt, Geraldine Kaeslin, Matthew A Cooper, Mark A T Blaskovich
Reduced susceptibility to antimicrobials in Gram-negative bacteria may result from multiple resistance mechanisms, including increased efflux pump activity or reduced porin protein expression. Up-regulation of the efflux pump system is closely associated with multidrug resistance (MDR). To help investigate the role of efflux pumps on compound accumulation, a fluorescence-based assay was developed using fluorescent derivatives of trimethoprim (TMP), a broad-spectrum synthetic antibiotic that inhibits an intracellular target, dihydrofolate reductase (DHFR)...
October 14, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27716055/tlr9-mediates-s-aureus-killing-inside-osteoblasts-via-induction-of-oxidative-stress
#13
Walid Mohamed, Eugen Domann, Trinad Chakraborty, Gopala Mannala, Katrin S Lips, Christian Heiss, Reinhard Schnettler, Volker Alt
BACKGROUND: Staphylococcus aureus is the principle causative pathogen of osteomyelitis and implant-associated bone infections. It is able to invade and to proliferate inside osteoblasts thus avoiding antibiotic therapy and the host immune system. Therefore, development of alternative approaches to stimulate host innate immune responses could be beneficial in prophylaxis against S. aureus infection. TLR9 is the intracellular receptor which recognizes unmethylated bacterial CpG-DNA and activates immune cells...
October 3, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27672351/establishment-of-a-simple-and-quick-method-for-detecting-extended-spectrum-%C3%AE-lactamase-esbl-genes-in-bacteria
#14
Song-Tao Han, Ying Fei, Jin-You Huang, Mei Xu, Li-Chan Chen, D Joshua Liao, Yu-Jie Tan
Extended-spectrum β-lactamase (ESBL) genes that render bacteria resistant to antibiotics are commonly detected using phenotype testing, which is time consuming and not sufficiently accurate. To establish a better method, we used phenotype testing to identify ESBL-positive bacterial strains and conducted PCR to screen for TEM (named after the patient Temoneira who provided the first sample), sulfhydryl reagent variable (SHV), cefotaxime (CTX)-M-1, and CTX-M-9, the 4 most common ESBL types and subtypes. We then performed multiplex PCR with 1 primer containing a biotin and hybridized the PCR products with gene-specific probes that were coupled with microbeads and coated with a specific fluorescence...
December 2016: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/27643682/alternative-fluorescent-labeling-strategies-for-characterizing-gram-positive-pathogenic-bacteria-flow-cytometry-supported-counting-sorting-and-proteome-analysis-of-staphylococcus-aureus-retrieved-from-infected-host-cells
#15
Petra Hildebrandt, Kristin Surmann, Manuela Gesell Salazar, Nicole Normann, Uwe Völker, Frank Schmidt
Staphylococcus aureus is a Gram-positive opportunistic pathogen that is able to cause a broad range of infectious diseases in humans. Furthermore, S. aureus is able to survive inside nonprofessional phagocytic host cell which serve as a niche for the pathogen to hide from the immune system and antibiotics therapies. Modern OMICs technologies provide valuable tools to investigate host-pathogen interactions upon internalization. However, these experiments are often hampered by limited capabilities to retrieve bacteria from such an experimental setting...
October 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27624932/modern-diagnostic-methods-for-urinary-tract-infections
#16
Moritz Fritzenwanker, Can Imirzalioglu, Trinad Chakraborty, Florian M Wagenlehner
Urinary tract infections (UTIs) are among the most common bacterial infections and constitute for a considerable number of all antibiotic prescriptions. The surge of multi-resistant bacteria has significantly complicated empirical treatment leading to wide use of broad-spectrum antibiotics, further driving resistance development. New ways to detect and quantify host and bacterial molecules in urine promise opportunities to improve diagnostics of UTIs, which can translate to new approaches in treatment regimens...
November 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27553650/effects-of-medium-chain-fatty-acids-on-the-structure-and-immune-response-of-ipec-j2-cells
#17
B Martínez-Vallespín, W Vahjen, J Zentek
Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine in vitro model using the porcine cell line IPEC-J2 was used to test the effects of MCFAs on structural and immunological traits without and with a concomitant challenge with E. coli or S. Enteritidis...
October 2016: Cytotechnology
https://www.readbyqxmd.com/read/27545347/the-spectrum-and-regulatory-landscape-of-intestinal-innate-lymphoid-cells-are-shaped-by-the-microbiome
#18
Meital Gury-BenAri, Christoph A Thaiss, Nicolas Serafini, Deborah R Winter, Amir Giladi, David Lara-Astiaso, Maayan Levy, Tomer Meir Salame, Assaf Weiner, Eyal David, Hagit Shapiro, Mally Dori-Bachash, Meirav Pevsner-Fischer, Erika Lorenzo-Vivas, Hadas Keren-Shaul, Franziska Paul, Alon Harmelin, Gérard Eberl, Shalev Itzkovitz, Amos Tanay, James P Di Santo, Eran Elinav, Ido Amit
Innate lymphoid cells (ILCs) are critical modulators of mucosal immunity, inflammation, and tissue homeostasis, but their full spectrum of cellular states and regulatory landscapes remains elusive. Here, we combine genome-wide RNA-seq, ChIP-seq, and ATAC-seq to compare the transcriptional and epigenetic identity of small intestinal ILCs, identifying thousands of distinct gene profiles and regulatory elements. Single-cell RNA-seq and flow and mass cytometry analyses reveal compartmentalization of cytokine expression and metabolic activity within the three classical ILC subtypes and highlight transcriptional states beyond the current canonical classification...
August 25, 2016: Cell
https://www.readbyqxmd.com/read/27530853/antimicrobial-action-of-zinc-oxide-nanoparticles-in-combination-with-ciprofloxacin-and-ceftazidime-against-multidrug-resistant-acinetobacter-baumannii
#19
F Ghasemi, R Jalal
Acinetobacter baumannii is a serious concern amongst hospitalised patients worldwide and its resistance to antibiotics has emerged as a threat to public health in recent years. Metal oxide nanoparticles were found to be effective for overcoming bacterial resistance owing to their antibacterial activities. The aim of this study was to investigate the combined effects of zinc oxide nanoparticles (ZnO-NPs) and the conventional antibiotics ciprofloxacin and ceftazidime as well as their mechanisms of action against resistant A...
September 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27507962/antibiotic-susceptibility-testing-of-the-gram-negative-bacteria-based-on-flow-cytometry
#20
Claude Saint-Ruf, Steve Crussard, Christine Franceschi, Sylvain Orenga, Jasmine Ouattara, Mahendrasingh Ramjeet, Jérémy Surre, Ivan Matic
Rapidly treating infections with adequate antibiotics is of major importance. This requires a fast and accurate determination of the antibiotic susceptibility of bacterial pathogens. The most frequently used methods are slow because they are based on the measurement of growth inhibition. Faster methods, such as PCR-based detection of determinants of antibiotic resistance, do not always provide relevant information on susceptibility, particularly that which is not genetically based. Consequently, new methods, such as the detection of changes in bacterial physiology caused by antibiotics using flow cytometry and fluorescent viability markers, are being explored...
2016: Frontiers in Microbiology
keyword
keyword
63206
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"