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Flow cytometry antibiotic

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https://www.readbyqxmd.com/read/29677546/bacitracin-promotes-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-by-stimulating-the-bone-morphogenetic-protein-2-smad-axis
#1
Hui Li, Bin'en Nie, Zhe Du, Shutao Zhang, Teng Long, Bing Yue
Bacitracin, a widely used metallopeptide antibiotic, has been reported to be locally used in treating wounds without systemic adverse reactions. Our preliminary study showed that bacitracin might enhance the osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). The present study investigated whether bacitracin affects the osteogenic differentiation of HBMSCs and the molecular mechanisms involved. The proliferation of HBMSCs in the presence of bacitracin was examined using a cell counting kit-8 (CCK-8) assay...
April 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29651185/tackling-salmonella-persister-cells-by-antibiotic-nisin-combination-via-mannitol
#2
Praveen Rishi, Neha Rani Bhagat, Reena Thakur, Preeti Pathania
Bacterial persisters (defined as dormant, non-dividing cells with globally reduced metabolism) are the major cause of recurrent infections. As they neither grow nor die in presence of antibiotics, it is difficult to eradicate these cells using antibiotics, even at higher concentrations. Reports of metabolites (which help in waking up of these inactive cells) enabled eradication of bacterial persistence by aminoglycosides, suggest the new potential strategy to improve antibiotic therapy. Here we propose, mannitol enabled elimination of Salmonella persister cells by the nisin-antibiotic combination...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29622805/generation-and-customization-of-biosynthetic-excitable-tissues-for-electrophysiological-studies-and-cell-based-therapies
#3
Hung X Nguyen, Robert D Kirkton, Nenad Bursac
We describe a two-stage protocol to generate electrically excitable and actively conducting cell networks with stable and customizable electrophysiological phenotypes. Using this method, we have engineered monoclonally derived excitable tissues as a robust and reproducible platform to investigate how specific ion channels and mutations affect action potential (AP) shape and conduction. In the first stage of the protocol, we combine computational modeling, site-directed mutagenesis, and electrophysiological techniques to derive optimal sets of mammalian and/or prokaryotic ion channels that produce specific AP shape and conduction characteristics...
May 2018: Nature Protocols
https://www.readbyqxmd.com/read/29613823/analysis-of-gene-expression-changes-in-pha-m-stimulated-lymphocytes-unraveling-pha-activity-as-prerequisite-for-dicentric-chromosome-analysis
#4
C Beinke, M Port, R Ullmann, K Gilbertz, M Majewski, M Abend
Dicentric chromosome analysis (DCA) is the gold standard for individual radiation dose assessment. However, DCA is limited by the time-consuming phytohemagglutinin (PHA)-mediated lymphocyte activation. In this study using human peripheral blood lymphocytes, we investigated PHA-associated whole genome gene expression changes to elucidate this process and sought to identify suitable gene targets as a means of meeting our long-term objective of accelerating cell cycle kinetics to reduce DCA culture time. Human peripheral whole blood from three healthy donors was separately cultured in RPMI/FCS/antibiotics with BrdU and PHA-M...
April 3, 2018: Radiation Research
https://www.readbyqxmd.com/read/29593544/the-influence-of-shuttle-shape-emodin-nanoparticles-on-the-streptococcus-suis-biofilm
#5
Wenya Ding, Jin Sun, He Lian, Changgeng Xu, Xin Liu, Sidi Zheng, Dong Zhang, Xiaopeng Han, Yanyan Liu, Xueying Chen, Bello O God Spower, Yanhua Li
Biofilm is one of the most important physiological protective barriers of the Streptococcus suis ( S. suis ), and it is also one of the primary causes of hindrance to drug infiltration, reduction of bactericidal effects, and the development of antibiotic resistance. In order to intervene or eliminate S. suis biofilm, shuttle-shape emodin-loaded nanoparticles were developed in our study. The emodin nanoparticles were prepared by emodin and gelatin-cyclodextrin which was synthesized as drug carrier, and the nanoparticles were 174 nm in size, -4...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29554697/secreted-immunomodulatory-proteins-of-staphylococcus-aureus-activate-platelets-and-induce-platelet-aggregation
#6
Ulrike Binsker, Raghavendra Palankar, Jan Wesche, Thomas P Kohler, Josephine Prucha, Gerhard Burchhardt, Manfred Rohde, Frank Schmidt, Barbara M Bröker, Uwe Mamat, Jan Pané-Farré, Anica Graf, Patrick Ebner, Andreas Greinacher, Sven Hammerschmidt
Staphylococcus aureus can cause bloodstream infections associated with infective endocarditis (IE) and disseminated intravascular coagulopathy (DIC). Both complications involve platelets. In view of an increasing number of antibiotic-resistant strains, new approaches to control systemic S. aureus infection are gaining importance. Using a repertoire of 52 recombinant S. aureus proteins in flow cytometry-based platelet activation and aggregation assays, we identified, in addition to the extracellular adherence protein Eap, three secreted staphylococcal proteins as novel platelet activating proteins...
March 19, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29514208/fitness-cost-of-mcr-1-mediated-polymyxin-resistance-in-klebsiella-pneumoniae
#7
Sue C Nang, Faye C Morris, Michael J McDonald, Mei-Ling Han, Jiping Wang, Richard A Strugnell, Tony Velkov, Jian Li
Objectives: The discovery of mobile colistin resistance mcr-1, a plasmid-borne polymyxin resistance gene, highlights the potential for widespread resistance to the last-line polymyxins. In the present study, we investigated the impact of mcr-1 acquisition on polymyxin resistance and biological fitness in Klebsiella pneumoniae. Methods: K. pneumoniae B5055 was used as the parental strain for the construction of strains carrying vector only (pBBR1MCS-5) and mcr-1 recombinant plasmids (pmcr-1)...
March 5, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29474463/the-development-and-validation-of-different-decision-making-tools-to-predict-urine-culture-growth-out-of-urine-flow-cytometry-parameter
#8
Martin Müller, Ruth Seidenberg, Sabine K Schuh, Aristomenis K Exadaktylos, Clyde B Schechter, Alexander B Leichtle, Wolf E Hautz
OBJECTIVE: Patients presenting with suspected urinary tract infection are common in every day emergency practice. Urine flow cytometry has replaced microscopic urine evaluation in many emergency departments, but interpretation of the results remains challenging. The aim of this study was to develop and validate tools that predict urine culture growth out of urine flow cytometry parameter. METHODS: This retrospective study included all adult patients that presented in a large emergency department between January and July 2017 with a suspected urinary tract infection and had a urine flow cytometry as well as a urine culture obtained...
2018: PloS One
https://www.readbyqxmd.com/read/29464325/detection-and-cell-sorting-of-pseudonocardia-species-by-fluorescence-in-situ-hybridization-and-flow-cytometry-using-16s-rrna-targeted-oligonucleotide-probes
#9
Mengyan Li, Yu Yang, Ya He, Jacques Mathieu, Cong Yu, Qilin Li, Pedro J J Alvarez
Pseudonocardia spp. are receiving increasing attention due to their ability to biodegrade recalcitrant cyclic ether pollutants (e.g., 1,4-dioxane and tetrahydrofuran), as well as for their distinctive ecological niches (e.g., symbiosis with ants/plants and production of antibiotics). Isolating and characterizing Pseudonocardia spp. is thus important to discern their metabolic and physiological idiosyncrasies and advance their potential applications. However, slow growth, low cell yield, and dissimilar colony morphology hinder efficient isolation of Pseudonocardia using conventional plating methods...
February 21, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29437871/gut-microbiota-modulate-t-cell-trafficking-into-human-colorectal-cancer
#10
Eleonora Cremonesi, Valeria Governa, Jesus Francisco Glaus Garzon, Valentina Mele, Francesca Amicarella, Manuele Giuseppe Muraro, Emanuele Trella, Virginie Galati-Fournier, Daniel Oertli, Silvio Raffael Däster, Raoul A Droeser, Benjamin Weixler, Martin Bolli, Raffaele Rosso, Ulrich Nitsche, Nina Khanna, Adrian Egli, Simone Keck, Julia Slotta-Huspenina, Luigi M Terracciano, Paul Zajac, Giulio Cesare Spagnoli, Serenella Eppenberger-Castori, Klaus-Peter Janssen, Lubor Borsig, Giandomenica Iezzi
OBJECTIVE: Tumour-infiltrating lymphocytes (TILs) favour survival in human colorectal cancer (CRC). Chemotactic factors underlying their recruitment remain undefined. We investigated chemokines attracting T cells into human CRCs, their cellular sources and microenvironmental triggers. DESIGN: Expression of genes encoding immune cell markers, chemokines and bacterial 16S ribosomal RNA (16SrRNA) was assessed by quantitative reverse transcription-PCR in fresh CRC samples and corresponding tumour-free tissues...
February 6, 2018: Gut
https://www.readbyqxmd.com/read/29434988/the-effects-of-hemocyanin-on-t-cells-cultured-in-vitro
#11
Jiani Liu, Cong Chen, Chen Ling, Haoyun Hu, Jingsong Cao, Yongqiang Gao
As a broad-spectrum antibiotic, gentamicin is used extensively in T cell culturing in vitro, but preliminary studies have identified that T cell activity is significantly affected by gentamicin. In the present study, the hemocyanin from Litopenaeus vannamei (L. vannamei) was selected as an additive for T cell cultures in vitro. Compared with those in the control group, the cell quantity exhibited no significant difference, and the formation rate of cell colony increased gradually with increases in the hemocyanin concentration...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29432913/effects-of-fluconazole-on-the-clinical-outcome-and-immune-response-in-fungal-co-infected-tuberculosis-patients
#12
Xiaojuan Ren, Wei Liu, Yi Liu
With overuse of the broad-spectrum antibiotics, the pulmonary fungal infection increasingly becomes the most common complication associated with senile pulmonary tuberculosis (TB) and attracts intensive attentions from clinicians. Here we presented the retrospective analysis of impact of fluconazole treatment on the clinical outcome and immune response in fungal co-infected tuberculosis patients. A randomized, double-blind, placebo-controlled trial of fluconazole (100 mg per day for consecutive weeks) in fungal-positive senile tuberculosis patients was conducted in our hospital...
February 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29423561/colistin-causes-profound-morphological-alteration-but-minimal-cytoplasmic-membrane-perforation-in-populations-of-escherichia-coli-and-pseudomonas-aeruginosa
#13
Noëlle H O'Driscoll, T P Tim Cushnie, Kerr H Matthews, Andrew J Lamb
Whilst colistin (polymyxin E) represents the last mainstream treatment option for multidrug-resistant Gram-negative pathogens, details of its mechanism of action remain to be fully resolved. In this study, the effects of sub-inhibitory, inhibitory-bactericidal, and supra-bactericidal levels of colistin on the membrane integrity and morphology of Escherichia coli and Pseudomonas aeruginosa were investigated using potassium loss, flow cytometry, and scanning electron microscopy (SEM). Supra-bactericidal colistin concentrations induced just 4-12% intracellular potassium loss from bacteria after 24 h...
February 8, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29422524/evolution-of-enzymes-with-new-specificity-by-high-throughput-screening-using-dmpr-based-genetic-circuits-and-multiple-flow-cytometry-rounds
#14
Kil Koang Kwon, Dae-Hee Lee, Su Jin Kim, Su-Lim Choi, Eugene Rha, Soo-Jin Yeom, Bindu Subhadra, Jinhyuk Lee, Ki Jun Jeong, Seung-Goo Lee
Genetic circuit-based biosensors are useful in detecting target metabolites or in vivo enzymes using transcription factors (Tx) as a molecular switch to express reporter signals, such as cellular fluorescence and antibiotic resistance. Herein, a phenol-detecting Tx (DmpR) was employed as a critical tool for enzyme engineering, specifically for the rapid analysis of numerous mutants with multiple mutations at the active site of tryptophan-indole lyase (TIL, EC 4.1.99.1). Cellular fluorescence was monitored cell-by-cell using flow cytometry to detect the creation of phenolic compounds by a new tyrosine-phenol-lyase (TPL, EC 4...
February 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29415902/influence-of-changes-in-the-intestinal-microflora-on-the-immune-function-in-mice
#15
Shigefumi Kishida, Yuko Kato-Mori, Katsuro Hagiwara
The composition of the intestinal microbiota is related to the health and immune function of the host. Administration of antibiotics affects the composition of the intestinal microbiota. However, the effects of immune function on the composition of the intestinal microbiota are still unclear. In this study, we investigated the lymphocyte composition and determined the relationships between lymphocyte function and the intestinal microbiota following antibiotic treatment in mice. To change the composition of the intestinal microbiota, mice were treated with or without antibiotics...
February 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29407425/evaluation-of-porcine-beta-defensins-1-and-2-as-antimicrobial-peptides-for-liquid-stored-boar-semen-effects-on-bacterial-growth-and-sperm-quality
#16
Adrià Puig-Timonet, Miriam Castillo-Martín, Barbara A Pereira, Elisabeth Pinart, Sergi Bonet, Marc Yeste
The present study evaluated whether two different antimicrobial peptides (AMP): porcine beta defensins-1 (PBD1) and -2 (PBD2) at three concentrations (1.5 μM, 3 μM and 5 μM) could be a suitable alternative to antibiotics in liquid-stored boar semen. Two separate experiments were conducted with liquid-stored boar semen preserved at 17 °C for 9-10 days. In the first one, we evaluated the impact of adding three concentrations of each AMP on the bacterial growth and sperm quality of boar semen stored for 10 days...
February 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/29385662/a-new-method-of-producing-a-natural-anti-bacterial-peptide-by-encapsulated-probiotics-internalized-with-inulin-nanoparticles-as-prebiotics
#17
Lian-Hua Cui, Chang-Guo Yan, Hui-Shan Li, Whee-Soo Kim, Liang Hong, Sang-Kee Kang, Yun-Jaie Choi, Chong-Su Cho
Synbiotics are a combination of probiotics and prebiotics, which lead to synergistic benefits in host welfare. Probiotics have been used as an alternative to antibiotics. Among the probiotics, Pediococcus acidilactici (PA) has shown excellent antimicrobial activity against Salmonella Gallinarum (SG) as a major poultry pathogen and has improved the production performances of animals. Inulin is widely used as a prebiotic for the improvement of animal health and growth. The main aim of this study is to investigate the effect of the antimicrobial activity of inulin nanoparticles (INs)-internalized PA encapsulated into alginate/chitosan/alginate (ACA) microcapsules (MCs) in future in vivo application...
February 1, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29363485/effect-and-mechanism-of-egfl7-downregulation-in-human-osteosarcoma-cells-on-the-biological-function-of-co-cultured-huvec
#18
Xia Li, Li Feng Liu, Yang Zhou Liu, Yu Tao Pan, Guang Li, Qing You Lu, Zeng Chun Li
BACKGROUND: Even though epidermal growth factor-like domain 7 is known to be overexpressed in osteosarcoma and is associated with poor clinical outcome, few reports are available regarding its mechanism. AIMS: The objective of this study was to explore the effect and mechanism of downregulating epidermal growth factor-like domain 7 expression in a human osteosarcoma cell line on the biological function of co-cultured human umbilical vein endothelial cells. STUDY DESIGN: Cell study...
March 15, 2018: Balkan Medical Journal
https://www.readbyqxmd.com/read/29360463/ceragenin-csa13-reduces-clostridium-difficile-infection-in-mice-by-modulating-the-intestinal-microbiome-and-metabolites
#19
Jiani Wang, Sally Ghali, Chunlan Xu, Caroline C Mussatto, Christina Ortiz, Elaine C Lee, Diana H Tran, Jonathan P Jacobs, Venu Lagishetty, Kym F Faull, Travis Moller, Maura Rossetti, Xinhua Chen, Hon Wai Koon
BACKGROUND & AIMS: Clostridium difficile induces intestinal inflammation by releasing toxins A and B. The antimicrobial compound cationic steroid antimicrobial 13 (CSA13) has been developed for treating gastrointestinal infections. The CSA13-Eudragit formulation can be given orally and releases CSA13 in the terminal ileum and colon. We investigated whether this form of CSA13 reduces C difficile infection (CDI) in mice. METHODS: C57BL/6J mice were infected with C difficile on day 0, followed by subcutaneous administration of pure CSA13 or oral administration of CSA13-Eudragit (10 mg/kg/d for 10 days)...
January 31, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29350524/development-of-a-multiplexed-microsphere-pcr-for-culture-free-detection-and-gram-typing-of-bacteria-in-human-blood-samples
#20
Fang Liang, Daniel J Browne, Megan J Gray, Kate H Gartlan, David D Smith, Ross T Barnard, Geoffrey R Hill, Simon R Corrie, Kate A Markey
Bloodstream infection is a significant clinical problem, particularly in vulnerable patient groups such as those undergoing chemotherapy and bone marrow transplantation. Clinical diagnostics for suspected bloodstream infection remain centered around blood culture (highly variable timing, in the order of hours to days to become positive), and empiric use of broad-spectrum antibiotics is therefore employed for patients presenting with febrile neutropenia. Gram-typing provides the first opportunity to target therapy (e...
January 31, 2018: ACS Infectious Diseases
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