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Microbial fermentation technology

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https://www.readbyqxmd.com/read/29761637/using-gas-mixtures-of-co-co-2-and-h-2-as-microbial-substrates-the-do-s-and-don-ts-of-successful-technology-transfer-from-laboratory-to-production-scale
#1
REVIEW
Ralf Takors, Michael Kopf, Joerg Mampel, Wilfried Bluemke, Bastian Blombach, Bernhard Eikmanns, Frank R Bengelsdorf, Dirk Weuster-Botz, Peter Dürre
The reduction of CO2 emissions is a global effort which is not only supported by the society and politicians but also by the industry. Chemical producers worldwide follow the strategic goal to reduce CO2 emissions by replacing existing fossil-based production routes with sustainable alternatives. The smart use of CO and CO2 /H2 mixtures even allows to produce important chemical building blocks consuming the said gases as substrates in carboxydotrophic fermentations with acetogenic bacteria. However, existing industrial infrastructure and market demands impose constraints on microbes, bioprocesses and products that require careful consideration to ensure technical and economic success...
May 14, 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29760349/identification-of-novel-potential-acetate-oxidizing-bacteria-in-an-acetate-fed-methanogenic-chemostat-based-on-dna-stable-isotope-probing
#2
Hui-Zhong Wang, Min Gou, Yue Yi, Zi-Yuan Xia, Yue-Qin Tang
Acetate is a significant intermediate of anaerobic fermentation. There are two pathways for converting acetate to CH4 and CO2 : acetoclastic methanogenesis by acetoclastic methanogens, and syntrophic acetate oxidation by acetate-oxidizing bacteria (AOB) and hydrogenotrophic methanogens. Detailed investigations of syntrophic acetate-oxidizing bacteria (SAOB) should contribute to the elucidation of the microbial mechanisms of methanogenesis. In this study, we investigated the major phylogenetic groups of acetate-utilizing bacteria (AUB) in a mesophilic methanogenic chemostat fed with acetate as the sole carbon source by using DNA stable isotope probing (SIP) technology...
May 11, 2018: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29755586/ethanol-production-potential-from-afex%C3%A2-and-steam-exploded-sugarcane-residues-for-sugarcane-biorefineries
#3
Thapelo Mokomele, Leonardo da Costa Sousa, Venkatesh Balan, Eugéne van Rensburg, Bruce E Dale, Johann F Görgens
Background: Expanding biofuel markets are challenged by the need to meet future biofuel demands and mitigate greenhouse gas emissions, while using domestically available feedstock sustainably. In the context of the sugar industry, exploiting under-utilized cane leaf matter (CLM) in addition to surplus sugarcane bagasse as supplementary feedstock for second-generation ethanol production has the potential to improve bioenergy yields per unit land. In this study, the ethanol yields and processing bottlenecks of ammonia fibre expansion (AFEX™) and steam explosion (StEx) as adopted technologies for pretreating sugarcane bagasse and CLM were experimentally measured and compared for the first time...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29750996/eco-friendly-microbial-production-of-diosgenin-from-saponins-in-dioscorea-zingiberensis-tubers-in-the-presence-of-aspergillus-awamori
#4
Yu Chen, Yi Dong, Yuanlong Chi, Qiang He, Hui Wu, Yao Ren
A novel microbial procedure was proposed for diosgenin production from Dioscorea zingiberensis C.H. Wright (DZW) tubers via employing Aspergillus awamori for the first time. The optimal conditions of fermenter cultivation were established as inoculation dosage of 8%, fermentation temperature of 30 °C, cultivation time of 8 days, initial pH of 7.0 and a stirring rate of 180 rpm when the converted diosgenin content reached a peak value of 74.26±3.23 mg/g substrate. The product was purified by silica gel column and then confirmed as diosgenin (purity: 96...
May 8, 2018: Steroids
https://www.readbyqxmd.com/read/29747085/study-on-feasibility-of-determination-of-glucosamine-content-of-fermentation-process-using-a-micro-nir-spectrometer
#5
Zhongyu Sun, Can Li, Lian Li, Lei Nie, Qin Dong, Danyang Li, Lingling Gao, Hengchang Zang
N-acetyl-d-glucosamine (GlcNAc) is a microbial fermentation product, and NIR spectroscopy is an effective process analytical technology (PAT) tool in detecting the key quality attribute: the GlcNAc content. Meanwhile, the design of NIR spectrometers is under the trend of miniaturization, portability and low-cost nowadays. The aim of this study was to explore a portable micro NIR spectrometer with the fermentation process. First, FT-NIR spectrometer and Micro-NIR 1700 spectrometer were compared with simulated fermentation process solutions...
May 2, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29738974/investigation-on-hydrogen-production-from-paper-sludge-without-inoculation-and-its-enhancement-by-clostridium-thermocellum
#6
Qian An, Ji-Lian Wang, Yu-Tao Wang, Zhang-Lin Lin, Ming-Jun Zhu
The feasibility and performance of hydrogen production from paper sludge without inoculation was investigated under thermophilic conditions. The maximum hydrogen production reached 64.32 mM with 7.4% PS. The dynamic changes in bacterial community structures during hydrogen production were investigated by analyzing 16S rDNA gene sequences using high throughput sequencing technology. The results showed that microbial community was dominated by order Clostridiales and Thermoanaerobacterales. Genus Thermoanaerobacterium and Ruminiclostridium played a leading role in the fermentation process, which was responsible for the hydrolysis of PS and hydrogen production...
April 30, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29728028/-research-progress-of-platelet-activating-factor-paf-receptor-antagonist
#7
Xue-Qing Li, Ting Geng, Wen-Zhe Huang, Zhen-Zhong Wang, Wei Xiao
Platelet activating factor(PAF), an endogenous synthesized phospholipid transmitter, has widely biological activities. It has "signal transmission" effect in various life processes, but abnormality of concentration in vivo will promote or aggravate the diseases, such as, cerebral ischemia, myocardial injury, multi-organ failure, asthma, injury of liver and kidney, severely affecting the normal life activities of body. In recent years, with the development of medical science and technology, more and more attention has been paid to the research of platelet activating factor receptor antagonist...
April 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29724034/an-improved-weighted-partial-least-squares-method-coupled-with-near-infrared-spectroscopy-for-rapid-determination-of-multiple-components-and-anti-oxidant-activity-of-pu-erh-tea
#8
Ze Liu, Hua-Lin Xie, Lin Chen, Jian-Hua Huang
Background: Pu-erh tea is a unique microbially fermented tea, which distinctive chemical constituents and activities are worthy of systematic study. Near infrared spectroscopy (NIR) coupled with suitable chemometrics approaches can rapidly and accurately quantitatively analyze multiple compounds in samples. Methods: In this study, an improved weighted partial least squares (PLS) algorithm combined with near infrared spectroscopy (NIR) was used to construct a fast calibration model for determining four main components, i...
May 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29719467/new-metabolites-from-the-biotransformation-of-ginsenoside-rb1-by-paecilomyces-bainier-sp-229-and-activities-in-inducing-osteogenic-differentiation-by-wnt-%C3%AE-catenin-signaling-activation
#9
Wei Zhou, Hai Huang, Haiyan Zhu, Pei Zhou, Xunlong Shi
Background: Ginseng is a well-known traditional Chinese medicine that has been widely used in a range of therapeutic and healthcare applications in East Asian countries. Microbial transformation is regarded as an effective and useful technology in modification of nature products for finding new chemical derivatives with potent bioactivities. In this study, three minor derivatives of ginsenoside compound K were isolated and the inducing effects in the Wingless-type MMTV integration site (Wnt) signaling pathway were also investigated...
April 2018: Journal of Ginseng Research
https://www.readbyqxmd.com/read/29715602/characterization-of-spoilage-markers-in-modified-atmosphere-packaged-iceberg-lettuce
#10
Angelos-Gerasimos Ioannidis, Frederiek-Maarten Kerckhof, Yasmine Riahi Drif, Mike Vanderroost, Nico Boon, Peter Ragaert, Bruno De Meulenaer, Frank Devlieghere
Fresh cut iceberg lettuce spoilage was studied considering the microbial and biochemical activity, the formation of volatile organic compounds (VOC) and consumer acceptability. Lettuce was packaged under three different packaging conditions and stored at 4 °C for 10 days: anaerobic packaging (ANAER), equilibrium modified atmosphere packaging with 3% O2 (EMAP) and perforated packages (AIR). Results indicated a clear distinction between packaging conditions. EMAP and AIR resulted in a short shelf life (≤5...
April 22, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29713527/characterization-of-the-catalytic-structure-of-plant-phytase-protein-tyrosine-phosphatase-like-phytase-and-histidine-acid-phytases-and-their-biotechnological-applications
#11
REVIEW
Alex Sander Rodrigues Cangussu, Deborah Aires Almeida, Raimundo Wagner de Souza Aguiar, Sidnei Emilio Bordignon-Junior, Kelvinson Fernandes Viana, Luiz Carlos Bertucci Barbosa, Edson Wagner da Silva Cangussu, Igor Viana Brandi, Augustus Caeser Franke Portella, Gil Rodrigues Dos Santos, Eliane Macedo Sobrinho, William James Nogueira Lima
Phytase plays a prominent role in monogastric animal nutrition due to its ability to improve phytic acid digestion in the gastrointestinal tract, releasing phosphorus and other micronutrients that are important for animal development. Moreover, phytase decreases the amounts of phytic acid and phosphate excreted in feces. Bioinformatics approaches can contribute to the understanding of the catalytic structure of phytase. Analysis of the catalytic structure can reveal enzymatic stability and the polarization and hydrophobicity of amino acids...
2018: Enzyme Research
https://www.readbyqxmd.com/read/29670593/technologies-and-trends-to-improve-table-olive-quality-and-safety
#12
REVIEW
Marco Campus, Nurcan Değirmencioğlu, Roberta Comunian
Table olives are the most widely consumed fermented food in the Mediterranean countries. Peculiar processing technologies are used to process olives, which are aimed at the debittering of the fruits and improvement of their sensory characteristics, ensuring safety of consumption at the same time. Processors demand for novel techniques to improve industrial performances, while consumers' attention for natural and healthy foods has increased in recent years. From field to table, new techniques have been developed to decrease microbial load of potential spoilage microorganisms, improve fermentation kinetics and ensure safety of consumption of the packed products...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29664912/metatranscriptome-analysis-of-the-microbial-fermentation-of-dietary-milk-proteins-in-the-murine-gut
#13
Floor Hugenholtz, Mark Davids, Jessica Schwarz, Michael Müller, Daniel Tomé, Peter Schaap, Guido J E J Hooiveld, Hauke Smidt, Michiel Kleerebezem
Undigestible food ingredients are converted by the microbiota into a large range of metabolites, predominated by short chain fatty acids (SCFA). These microbial metabolites are subsequently available for absorption by the host mucosa and can serve as an energy source. Amino acids fermentation by the microbiota expands the spectrum of fermentation end-products beyond acetate, propionate and butyrate, to include in particular branched-SCFA. Here the long-term effects of high protein-diets on microbial community composition and functionality in mice were analyzed...
2018: PloS One
https://www.readbyqxmd.com/read/29659515/can-zymomonas-mobilis-substitute-saccharomyces-cerevisiae-in-cereal-dough-leavening
#14
Alida Musatti, Chiara Mapelli, Manuela Rollini, Roberto Foschino, Claudia Picozzi
Baker’s yeast intolerance is rising among Western populations, where Saccharomyces cerevisiae is spread in fermented food and food components. Zymomonas mobilis is a bacterium commonly used in tropical areas to produce alcoholic beverages, and it has only rarely been considered for dough leavening probably because it only ferments glucose, fructose and sucrose, which are scarcely present in flour. However, through alcoholic fermentation, similarly to S. cerevisiae , it provides an equimolar mixture of ethanol and CO₂ that can rise a dough...
April 16, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29627853/insect-symbionts-as-valuable-grist-for-the-biotechnological-mill-an-alkaliphilic-silkworm-gut-bacterium-for-efficient-lactic-acid-production
#15
Xili Liang, Chao Sun, Bosheng Chen, Kaiqian Du, Ting Yu, Vijitra Luang-In, Xingmeng Lu, Yongqi Shao
Insects constitute the most abundant and diverse animal class and act as hosts to an extraordinary variety of symbiotic microorganisms. These microbes living inside the insects play critical roles in host biology and are also valuable bioresources. Enterococcus mundtii EMB156, isolated from the larval gut (gut pH >10) of the model organism Bombyx mori (Lepidoptera: Bombycidae), efficiently produces lactic acid, an important metabolite for industrial production of bioplastic materials. E. mundtii EMB156 grows well under alkaline conditions and stably converts various carbon sources into lactic acid, offering advantages in downstream fermentative processes...
April 7, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29579944/characterization-of-microbial-community-structure-and-metabolic-potential-using-illumina-miseq-platform-during-the-black-garlic-processing
#16
Zhichang Qiu, Ningyang Li, Xiaoming Lu, Zhenjia Zheng, Mingjie Zhang, Xuguang Qiao
Black garlic is a distinctive garlic deep-processed product made from fresh garlic at high temperature and controlled humidity. To explore microbial community structure, diversity and metabolic potential during the 12days of the black garlic processing, Illumina MiSeq sequencing technology was performed to sequence the 16S rRNA V3-V4 hypervariable region of bacteria. A total of 677,917 high quality reads were yielded with an average read length of 416bp. Operational taxonomic units (OTU) clustering analysis showed that the number of species OTUs ranged from 148 to 1974, with alpha diversity increasing remarkably, indicating the high microbial community abundance and diversity...
April 2018: Food Research International
https://www.readbyqxmd.com/read/29561196/modulation-of-colonic-hydrogen-sulfide-production-by-diet-and-mesalazine-utilizing-a-novel-gas-profiling-technology
#17
Chu K Yao, Asaf Rotbart, Jian Z Ou, Kourosh Kalantar-Zadeh, Jane G Muir, Peter R Gibson
Excessive hydrogen sulfide (H2 S) production from gut microbial metabolism may have clinically important relevance in the pathogenesis of gut disorders, including ulcerative colitis. However, little is known regarding factors that alter its production. Using a newly-designed in vitro gas-profiling technology, the study aimed to verify real-time H2 S measurement reproducibility and thereafter, assess its production following exposure to dietary factors and 5-aminosalicylate acid (5-ASA). Measurements of H2 S, carbon dioxide, hydrogen and methane measurements were compared between gas-profiling systems...
March 21, 2018: Gut Microbes
https://www.readbyqxmd.com/read/29559966/unique-microbial-diversity-and-metabolic-pathway-features-of-fermented-vegetables-from-hainan-china
#18
Qiannan Peng, Shuaiming Jiang, Jieling Chen, Chenchen Ma, Dongxue Huo, Yuyu Shao, Jiachao Zhang
Fermented vegetables are typically traditional foods made of fresh vegetables and their juices, which are fermented by beneficial microorganisms. Herein, we applied high-throughput sequencing and culture-dependent technology to describe the diversities of microbiota and identify core microbiota in fermented vegetables from different areas of Hainan Province, and abundant metabolic pathways in the fermented vegetables were simultaneously predicted. At the genus level, Lactobacillus bacteria were the most abundant...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29558790/processes-and-electron-flow-in-a-microbial-electrolysis-cell-bioanode-fed-with-furanic-and-phenolic-compounds
#19
Xiaofei Zeng, Abhijeet P Borole, Spyros G Pavlostathis
Furanic and phenolic compounds are problematic compounds resulting from the pretreatment of lignocellulosic biomass for biofuel production. Microbial electrolysis cell (MEC) is a promising technology to convert furanic and phenolic compounds to renewable H2 . The objective of the research presented here was to elucidate the processes and electron equivalents flow during the conversion of two furanic (furfural, FF; 5-hydroxymethyl furfural, HMF) and three phenolic (syringic acid, SA; vanillic acid, VA; 4-hydroxybenzoic acid, HBA) compounds in the MEC bioanode...
March 20, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29557518/metabolic-engineering-of-corynebacterium-glutamicum-for-fermentative-production-of-chemicals-in-biorefinery
#20
REVIEW
Kei-Anne Baritugo, Hee Taek Kim, Yokimiko David, Jong-Il Choi, Soon Ho Hong, Ki Jun Jeong, Jong Hyun Choi, Jeong Chan Joo, Si Jae Park
Bio-based production of industrially important chemicals provides an eco-friendly alternative to current petrochemical-based processes. Because of the limited supply of fossil fuel reserves, various technologies utilizing microbial host strains for the sustainable production of platform chemicals from renewable biomass have been developed. Corynebacterium glutamicum is a non-pathogenic industrial microbial species traditionally used for L-glutamate and L-lysine production. It is a promising species for industrial production of bio-based chemicals because of its flexible metabolism that allows the utilization of a broad spectrum of carbon sources and the production of various amino acids...
March 20, 2018: Applied Microbiology and Biotechnology
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