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Microbial fermentation technology

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https://www.readbyqxmd.com/read/28887527/pan-genome-analysis-of-bacillus-for-microbiome-profiling
#1
Yihwan Kim, InSong Koh, Mi Young Lim, Won-Hyong Chung, Mina Rho
Recent advances in high-throughput sequencing technology allow for in-depth studies on microbial genomes and their communities. While multiple strains of the same species could display genomic variations with different gene contents in diverse habitats and hosts, the essential functions for a specific species are conserved as core genes that are shared among strains. We have comprehensively analyzed 238 strains of five different Bacillus species to identify the properties of core and strain-specific genes. Core and strain-specific genes in each Bacillus species show significant differences in their functions and genomic signatures...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28884354/synthetic-biology-for-manufacturing-chemicals-constraints-drive-the-use-of-non-conventional-microbial-platforms
#2
REVIEW
Jeffrey Czajka, Qinhong Wang, Yechun Wang, Yinjie J Tang
Genetically modified microbes have had much industrial success producing protein-based products (such as antibodies and enzymes). However, engineering microbial workhorses for biomanufacturing of commodity compounds remains challenging. First, microbes cannot afford burdens with both overexpression of multiple enzymes and metabolite drainage for product synthesis. Second, synthetic circuits and introduced heterologous pathways are not yet as "robust and reliable" as native pathways due to hosts' innate regulations, especially under suboptimal fermentation conditions...
September 7, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28857665/efficient-bioconversion-from-acid-hydrolysate-of-waste-oleaginous-yeast-biomass-after-microbial-oil-extraction-to-bacterial-cellulose-by-komagataeibacter-xylinus
#3
Mu-Tan Luo, Chao Huang, Xue-Fang Chen, Qian-Lin Huang, Gao-Xiang Qi, Lan-Lan Tian, Lian Xiong, Hai-Long Li, Xin-De Chen
Biomass acid hydrolysate of oleaginous yeast Trichosporon cutaneum after microbial oil extraction was applied as substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus (also named as Gluconacetobacter xylinus previously) for the first time. BC was synthesized in static culture for ten days and the maximum BC yield (2.9 g/ L) was got at the 4th day of fermentation. Most carbon sources in the substrate (glucose, mannose, formic acid, acetic acid, etc.) can be utilized by K. xylinus...
August 31, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28847079/gentamicin-degradation-and-changes-in-fungal-diversity-and-physicochemical-properties-during-composting-of-gentamicin-production-residue
#4
Yuanwang Liu, Yao Feng, Dengmiao Cheng, Jianming Xue, Steve A Wakelin, Haiyan Hu, Zhaojun Li
An indoor co-composting of gentamicin fermentation residues (GFR) and lovastatin fermentation residues (LFR) inoculated with gentamicin-degrading Aspergillus terreus FZC3 was conducted to remove gentamicin residues. The results showed that treatment MFZC3, consisting of a 10:1 blend of GFR and LFR (w/w), had the longest thermophilic phase (7days), quickest gentamicin degradation (t½=4.4days), and relatively higher gentamicin degradation percentage (96.7%) at the end of composting. Addition of Aspergillus terreus FZC3 affected fungal diversity of the compost and improved the removal of gentamicin during composting of the 15:1 GFR:LFR blend...
August 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28838223/determination-and-variation-of-core-bacterial-community-in-a-two-stage-full-scale-anaerobic-reactor-treating-high-strength-pharmaceutical-wastewater
#5
Haijun Ma, Lin Ye, Haidong Hu, Lulu Zhang, Lili Ding, Hongqiang Ren
The functional characterization and temporal variation of anaerobic bacterial population is important to better understanding of microbial process of two-stage anaerobic reactor. However, due to the high diversity of anaerobic bacteria, close attention should be prioritized to be paid to the frequently abundant bacteria that were defined as core bacteria and putatively functionally important. Here in this study, using Miseq sequencing technology, the core bacterial community of 98 operational taxonomic units (OTUs) was determined in a two-stage upflow blanket filter reactors treating pharmaceutical wastewater...
August 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28831702/microbial-community-composition-and-electricity-generation-in-cattle-manure-slurry-treatment-using-microbial-fuel-cells-effects-of-inoculum-addition
#6
Binghan Xie, Weijia Gong, An Ding, Huarong Yu, Fangshu Qu, Xiaobin Tang, Zhongsen Yan, Guibai Li, Heng Liang
Microbial fuel cell (MFC) is a sustainable technology to treat cattle manure slurry (CMS) for converting chemical energy to bioelectricity. In this work, two types of allochthonous inoculum including activated sludge (AS) and domestic sewage (DS) were added into the MFC systems to enhance anode biofilm formation and electricity generation. Results indicated that MFCs (AS + CMS) obtained the maximum electricity output with voltage approaching 577 ± 7 mV (~ 196 h), followed by MFCs (DS + CMS) (520 ± 21 mV, ~ 236 h) and then MFCs with autochthonous inoculum (429 ± 62 mV, ~ 263...
August 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28800557/predicting-and-experimental-evaluating-bio-electrochemical-synthesis-a-case-study-with-clostridium-kluyveri
#7
Christin Koch, Anne Kuchenbuch, Frauke Kracke, Paul V Bernhardt, Jens Krömer, Falk Harnisch
Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central for its further development is using appropriate electroactive microorganisms and their efficient extracellular electron transfer (EET) as well as wiring of the metabolism to EET. Among others, Clostridia are believed to represent electroactive microbes being highly promising for microbial electrosynthesis...
July 25, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28769888/unraveling-core-functional-microbiota-in-traditional-solid-state-fermentation-by-high-throughput-amplicons-and-metatranscriptomics-sequencing
#8
Zhewei Song, Hai Du, Yan Zhang, Yan Xu
Fermentation microbiota is specific microorganisms that generate different types of metabolites in many productions. In traditional solid-state fermentation, the structural composition and functional capacity of the core microbiota determine the quality and quantity of products. As a typical example of food fermentation, Chinese Maotai-flavor liquor production involves a complex of various microorganisms and a wide variety of metabolites. However, the microbial succession and functional shift of the core microbiota in this traditional food fermentation remain unclear...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28766035/valuable-biochemical-production-in-mixed-culture-fermentation-fundamentals-and-process-coupling
#9
REVIEW
Kun Dai, Jun-Li Wen, Fang Zhang, Raymond J Zeng
The mixed culture fermentation is an important environmental biotechnology that converts biodegradable organic wastes to valuable chemicals such as hydrogen, methane, acetate, ethanol, propionate, and so on. For the multistep process of hydrolysis, acidogenesis, acetogenesis/homoacetogensis, and methanogenesis, the typical metabolic reactions are firstly summarized. And then, since the final metabolites are always a mixture, the separation and purification processes are necessary to couple with anaerobic fermentation...
August 2, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28764108/cellulase-and-xylanase-production-at-pilot-scale-by-solid-state-fermentation-from-coffee-husk-using-specialized-consortia-the-consistency-of-the-process-and-the-microbial-communities-involved
#10
Alejandra Cerda, Laura Mejías, Teresa Gea, Antoni Sánchez
Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale...
July 16, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28754020/in-situ-%C3%AE-glucan-fortification-of-cereal-based-matrices-by-pediococcus-parvulus-2-6-technological-aspects-and-prebiotic-potential
#11
Adrián Pérez-Ramos, María Luz Mohedano, Paloma López, Giuseppe Spano, Daniela Fiocco, Pasquale Russo, Vittorio Capozzi
Bacterial exopolysaccharides produced by lactic acid bacteria are of increasing interest in the food industry, since they might enhance the technological and functional properties of some edible matrices. In this work, Pediococcus parvulus 2.6, which produces an O2-substituted (1,3)-β-d-glucan exopolysaccharide only synthesised by bacteria, was proposed as a starter culture for the production of three cereal-based fermented foods. The obtained fermented matrices were naturally bio-fortified in microbial β-glucans, and used to investigate the prebiotic potential of the bacterial exopolysaccharide by analysing the impact on the survival of a probiotic Lactobacillus plantarum strain under starvation and gastrointestinal simulated conditions...
July 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28732554/microbial-ecology-and-process-technology-of-sourdough-fermentation
#12
REVIEW
Luc De Vuyst, Simon Van Kerrebroeck, Frédéric Leroy
From a microbiological perspective, sourdough is to be considered as a specific and stressful ecosystem, harboring yeasts and lactic acid bacteria (LAB), that is used for the production of baked goods. With respect to the metabolic impact of the sourdough microbiota, acidification (LAB), flavor formation (LAB and yeasts), and leavening (yeasts and heterofermentative LAB species) are most noticeable. Three distinct types of sourdough fermentation processes can be discerned based on the inocula applied, namely backslopped ones (type 1), those initiated with starter cultures (type 2), and those initiated with a starter culture followed by backslopping (type 3)...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28725266/highly-efficient-methane-generation-from-untreated-microalgae-biomass
#13
Viktor Klassen, Olga Blifernez-Klassen, Daniel Wibberg, Anika Winkler, Jörn Kalinowski, Clemens Posten, Olaf Kruse
BACKGROUND: The fact that microalgae perform very efficiently photosynthetic conversion of sunlight into chemical energy has moved them into the focus of regenerative fuel research. Especially, biogas generation via anaerobic digestion is economically attractive due to the comparably simple apparative process technology and the theoretical possibility of converting the entire algal biomass to biogas/methane. In the last 60 years, intensive research on biogas production from microalgae biomass has revealed the microalgae as a rather challenging substrate for anaerobic digestion due to its high cell wall recalcitrance and unfavorable protein content, which requires additional pretreatment and co-fermentation strategies for sufficient fermentation...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28725194/sophisticated-cloning-fermentation-and-purification-technologies-for-an-enhanced-therapeutic-protein-production-a-review
#14
REVIEW
Sanjeev K Gupta, Pratyoosh Shukla
The protein productions strategies are crucial towards the development of application based research and elucidating the novel purification strategies for industrial production. Currently, there are few innovative avenues are studies for cloning, upstream, and purification through efficient bioprocess development. Such strategies are beneficial for industries as well as proven to be vital for effectual therapeutic protein development. Though, these techniques are well documented, but, there is scope of addition to current knowledge with novel and new approaches and it will pave new avenues in production of recombinant microbial and non-microbial proteins including secondary metabolites...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28696037/unravelling-biocomplexity-of-electroactive-biofilms-for-producing-hydrogen-from-biomass
#15
Alex J Lewis, Maria F Campa, Terry C Hazen, Abhijeet P Borole
Leveraging nature's biocomplexity for solving human problems requires better understanding of the syntrophic relationships in engineered microbiomes developed in bioreactor systems. Understanding the interactions between microbial players within the community will be key to enhancing conversion and production rates from biomass streams. Here we investigate a bioelectrochemical system employing an enriched microbial consortium for conversion of a switchgrass-derived bio-oil aqueous phase (BOAP) into hydrogen via microbial electrolysis (MEC)...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28690680/from-grass-to-gas-microbiome-dynamics-of-grass-biomass-acidification-under-mesophilic-and-thermophilic-temperatures
#16
Christian Abendroth, Claudia Simeonov, Juli Peretó, Oreto Antúnez, Raquel Gavidia, Olaf Luschnig, Manuel Porcar
BACKGROUND: Separating acidification and methanogenic steps in anaerobic digestion processes can help to optimize the process and contribute to producing valuable sub-products such as methane, hydrogen and organic acids. However, the full potential of this technology has not been fully explored yet. To assess the underlying fermentation process in more detail, a combination of high-throughput sequencing and proteomics on the acidification step of plant material (grass) at both mesophilic and thermophilic temperatures (37 and 55 °C, respectively) was applied for the first time...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28642742/microbial-resources-and-enological-significance-opportunities-and-benefits
#17
REVIEW
Leonardo Petruzzi, Vittorio Capozzi, Carmen Berbegal, Maria R Corbo, Antonio Bevilacqua, Giuseppe Spano, Milena Sinigaglia
Among the innovative trends in the wine sector, the continuous exploration of enological properties associated with wine microbial resources represents a cornerstone driver of quality improvement. Since the advent of starter cultures technology, the attention has been focused on intraspecific biodiversity within the primary species responsible for alcoholic fermentation (Saccharomyces cerevisiae) and, subsequently, for the so-called 'malolactic fermentation' (Oenococcus oeni). However, in the last decade, a relevant number of studies proposed the enological exploitation of an increasing number of species (e...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28622378/gene-modification-of-the-acetate-biosynthesis-pathway-in-escherichia-coli-and-implementation-of-the-cell-recycling-technology-to-increase-l-tryptophan-production
#18
Qingyang Xu, Fang Bai, Ning Chen, Gang Bai
The implementation of a novel cell recycling technology based on a special disk centrifuge during microbial fermentation process can continuously separate the product and harmful intermediates, while maintaining the cell viability owing to the installed cooling system. Acetate accumulation is an often encountered problem in L-tryptophan fermentation by Escherichia coli. To extend our previous studies, the current study deleted the key genes underlying acetate biosynthesis to improve l-tryptophan production...
2017: PloS One
https://www.readbyqxmd.com/read/28616071/combined-de-novo-and-ex-novo-lipid-fermentation-in-a-mix-medium-of-corncob-acid-hydrolysate-and-soybean-oil-by-trichosporon-dermatis
#19
Chao Huang, Mu-Tan Luo, Xue-Fang Chen, Gao-Xiang Qi, Lian Xiong, Xiao-Qing Lin, Can Wang, Hai-Long Li, Xin-De Chen
BACKGROUND: Microbial oil is one important bio-product for its important function in energy, chemical, and food industry. Finding suitable substrates is one key issue for its industrial application. Both hydrophilic and hydrophobic substrates can be utilized by oleaginous microorganisms with two different bio-pathways ("de novo" lipid fermentation and "ex novo" lipid fermentation). To date, most of the research on lipid fermentation has focused mainly on only one fermentation pathway and little work was carried out on both "de novo" and "ex novo" lipid fermentation simultaneously; thus, the advantages of both lipid fermentation cannot be fulfilled comprehensively...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28572938/the-effect-of-proteolytic-activity-of-starter-cultures-on-technologically-important-properties-of-yogurt
#20
Elahe Amani, Mohammad Hadi Eskandari, Shahram Shekarforoush
In this study, the effects of proteolytic activity of yogurt starter bacteria on physicochemical and technological properties of yogurt were investigated. Moreover, impact of proteolytic activity and production of exopolysaccharide (EPS) on the performance of each strain were screened. In order to compare the textural properties of yogurt samples, four parameters were evaluated: syneresis, water-holding capacity, cohesiveness, and hardness. Results showed that strains with high proteolytic activity had lower acidifying activity during fermentation and storage...
May 2017: Food Science & Nutrition
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