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Proanthocyanidin

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https://www.readbyqxmd.com/read/28107780/myb12-and-myb22-play-essential-roles-in-proanthocyanidin-and-flavonol-synthesis-in-red-fleshed-apple-malus-sieversii-f-niedzwetzkyana
#1
Nan Wang, Haifeng Xu, Shenghui Jiang, Zongying Zhang, Ninglin Lu, Huarong Qiu, Changzhi Qu, Yicheng Wang, Shujing Wu, Xuesen Chen
Flavonoids are major polyphenol compounds in plant secondary metabolism. Compared with cultivated apples, wild red-fleshed apples (Malus sieversii f. niedzwetzkyana) are excellent resources because of their high flavonoid content. In this work, R6R6, R6R1 and R1R1 genotypes were identified in an F1 segregating population of M. sieversii f. niedzwetzkyana. Significant differences in flavonoid compositions and contents were detected among the three genotypes by UPLC-ESI-TOF/MS analysis. Furthermore, two putative flavonoid-related genes encoding R2R3-MYB transcription factors, designated MYB12 and MYB22, were cloned and characterized...
January 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28106710/banana-passion-fruit-passiflora-mollissima-kunth-l-h-bailey-microencapsulation-phytochemical-composition-and-antioxidant-capacity
#2
Almudena García-Ruiz, Amadeo Girones-Vilaplana, Paola León, Diego A Moreno, Carla M Stinco, Antonio J Meléndez-Martínez, Jenny Ruales
Passiflora mollissima (Kunth) L.H. Bailey is an exotic fruit native to South America, known as taxo in Ecuador. This paper characterizes its flavonoid and carotenoid composition and antioxidant capacity and evaluates the effect of the spray-drying process on its phytochemical composition and antioxidant capacity. A total of 18 flavonoid compounds, nine proanthocyanidins and nine flavan-3-ol monomers, were identified and quantified. Glycosides of (epi)-afzelechin stood out as the most abundant flavonoid. Three carotenoids were identified, with β-carotene having the highest concentration...
January 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28100853/antioxidant-and-antimicrobial-properties-of-bioactive-phytochemicals-from-cranberry
#3
Monika Baranowska, Agnieszka Bartoszek
In the rational human diet, the important role of fruits and vegetables, which are a source of bioactive phytochemicals, is emphasized. Among fruits particular attention, due to a number of documented health-promoting properties, is focused on cranberry. This fruit is characterized by the high content of antioxidant phenolic compounds, which may support the natural antioxidant defense system of the body in the prevention of damage caused by oxidative stress induced by reactive oxygen species (ROS). Therefore, cranberry is suggested for the prevention of civilization diseases such as atherosclerosis, hypertension and cancer, whose etiology is associated directly with oxidative stress...
December 31, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28100511/higher-dietary-flavonoid-intakes-are-associated-with-lower-objectively-measured-body-composition-in-women-evidence-from-discordant-monozygotic-twins
#4
Amy Jennings, Alex MacGregor, Tim Spector, Aedín Cassidy
BACKGROUND: Although dietary flavonoid intake has been associated with less weight gain, there are limited data on its impact on fat mass, and to our knowledge, the contribution of genetic factors to this relation has not previously been assessed. OBJECTIVE: We examined the associations between flavonoid intakes and fat mass. DESIGN: In a study of 2734 healthy, female twins aged 18-83 y from the TwinsUK registry, intakes of total flavonoids and 7 subclasses (flavanones, anthocyanins, flavan-3-ols, flavonols, flavones, polymers, and proanthocyanidins) were calculated with the use of food-frequency questionnaires...
January 18, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28098463/absolute-configuration-of-native-oligomeric-proanthocyanidins-with-dentin-biomodification-potency
#5
Joo-Won Nam, Rasika S Phansalkar, David C Lankin, James B McAlpine, Ariene A Leme-Kraus, Cristina M P Vidal, Li-She Gan, Ana Bedran-Russo, Shao-Nong Chen, Guido F Pauli
The structurally complex oligomeric proanthocyanidins (OPACs) are promising biomimetic agents, capable of strengthening the macromolecular backbone of teeth via intermolecular and intermicrofibrillar cross-linking. This study establishes analytical methods capable of determining the absolute configuration of the catechin-type monomeric units of underivatized OPACs. This preserves the capacity of their biological evaluation, aimed at understanding the inevitably stereospecific interactions between the OPACs and dentin collagen...
January 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28095510/screening-and-identifying-antioxidative-components-in-ginkgo-biloba-pollen-by-dpph-hplc-pad-coupled-with-hplc-esi-ms2
#6
Jiying Qiu, Xiangyan Chen, A I Netrusov, Qingxin Zhou, Danyang Guo, Xiaoyong Liu, Hailun He, Xue Xin, Yifen Wang, Leilei Chen
The Ginkgo biloba is one of ancient trees that exists from billions of years ago, its leaf and nut are used as herbs and foods in China, while so far its pollen does not have any application except pollination. In order to evaluate the antioxidant activity of Ginkgo biloba pollen, and rapidly screen its antioxidative components, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, total flavonoid, total phenol, and proanthocyanidin of Ginkgo biloba pollen were determined and compared with those of Ginkgo biloba leaf and nut, and the off-line DPPH-HPLC-PAD and HPLC-ESI-MS2 were applied for screening and identifying the antioxidant flavonoids in Ginkgo biloba pollen...
2017: PloS One
https://www.readbyqxmd.com/read/28078420/comprehensive-polyphenol-profiling-of-a-strawberry-extract-fragaria-%C3%A3-ananassa-by-ultra-high-performance-liquid-chromatography-coupled-with-high-resolution-mass-spectrometry
#7
Giorgia La Barbera, Anna Laura Capriotti, Chiara Cavaliere, Susy Piovesana, Roberto Samperi, Riccardo Zenezini Chiozzi, Aldo Laganà
The aim of metabolic untargeted profiling is to detect and identify unknown compounds in a biological matrix to achieve the most comprehensive metabolic coverage. In phytochemical mixtures, however, the complexity of the sample could present significant difficulties in compound identification. In this case, the optimization of both the chromatographic and the mass-spectrometric conditions is supposed to be crucial for the detection and identification of the largest number of compounds. In this work, a systematic investigation of different chromatographic and mass-spectrometric conditions is presented to achieve a comprehensive untargeted profiling of a strawberry extract (Fragaria × ananassa)...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28076425/the-fatty-acid-profile-and-oxidative-stability-of-meat-from-turkeys-fed-diets-enriched-with-n-3-polyunsaturated-fatty-acids-and-dried-fruit-pomaces-as-a-source-of-polyphenols
#8
Jerzy Juskiewicz, Jan Jankowski, Henryk Zielinski, Zenon Zdunczyk, Dariusz Mikulski, Zofia Antoszkiewicz, Monika Kosmala, Przemyslaw Zdunczyk
The aim of this study was to determine the efficacy of different dietary fruit pomaces in reducing lipid oxidation in the meat of turkeys fed diets with a high content of n-3 polyunsaturated fatty acids (PUFAs). Over a period of 4 weeks before slaughter, turkeys were fed diets with the addition of 5% dried apple, blackcurrant, strawberry and seedless strawberry pomaces (groups AP, BP, SP and SSP, respectively) and 2.5% linseed oil. Pomaces differed in the content (from 5.5 in AP to 43.1 mg/g in SSP) and composition of polyphenols Proanthocyanidins were the main polyphenolic fraction in all pomaces, AP contained flavone glycosides and dihydrochalcones, BP contained anthocyanins, and SP and SSP-ellagitannins...
2017: PloS One
https://www.readbyqxmd.com/read/28040178/blueberry-proanthocyanidins-against-human-norovirus-surrogates-in-model-foods-and-under-simulated-gastric-conditions
#9
Snehal Joshi, Amy B Howell, Doris H D'Souza
Blueberry proanthocyanidins (B-PAC) are known to decrease titers of human norovirus surrogates in vitro. The application of B-PAC as therapeutic or preventive options against foodborne viral illness needs to be determined using model foods and simulated gastric conditions in vitro. The objective of this study was to evaluate the antiviral effect of B-PAC in model foods (apple juice (AJ) and 2% reduced fat milk) and simulated gastrointestinal fluids against cultivable human norovirus surrogates (feline calicivirus; FCV-F9 and murine norovirus; MNV-1) over 24 h at 37 °C...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28034505/high-performance-thin-layer-chromatography-mass-spectrometry-of-japanese-knotweed-flavan-3-ols-and-proanthocyanidins-on-silica-gel-plates
#10
Vesna Glavnik, Irena Vovk, Alen Albreht
On-line elution based TLC-MS is now a well-established technique, but the quality of the data obtained can sometimes be hampered by a severe spectral background or by strong ion suppression, especially when silica gel plates are used in combination with an acidic modifier in the developing solvent. We solved this issue simply and efficiently using two pre-developments of the plates, firstly with methanol-formic acid (10:1, v/v) and secondly with acetonitrile-methanol (2:1, v/v). This solution resulted in significant improvement in the sensitivity of HPTLC-MS methods...
December 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28029227/physicochemical-antioxidant-and-cytotoxic-properties-of-xao-tam-phan-paramignya-trimera-root-extract-and-its-fractions
#11
Nguyen Van Tang, Jennette A Sakoff, Christopher J Scarlett
Xao tam phan (Paramignya trimera (Oliv.) Guillaum) has been used as a medicinal plant for cancer prevention and treatment in recent years. The objective of this study was to determine the physicochemical, antioxidant and cytotoxic properties of crude P. trimera root (PTR) extract and its fractions using methanol as a solvent and microwave-assisted extraction as an advanced technique for preparation of the PTR extract. The results showed that the PTR extract had high contents of saponins, phenolics, flavonoids and proanthocyanidins (7731...
December 28, 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28018375/genome-wide-identification-localization-and-expression-analysis-of-proanthocyanidin-associated-genes-in-brassica
#12
Xianjun Liu, Ying Lu, Mingli Yan, Donghong Sun, Xuefang Hu, Shuyan Liu, Sheyuan Chen, Chunyun Guan, Zhongsong Liu
Proanthocyanidins (PA) is a type of prominent flavonoid compound deposited in seed coats which controls the pigmentation in all Brassica species. Annotation of Brassica juncea genome survey sequences showed 72 PA genes; however, a functional description of these genes, especially how their interactions regulate seed pigmentation, remains elusive. In the present study, we designed 19 primer pairs to screen a bacterial artificial chromosome (BAC) library of B. juncea. A total of 284 BAC clones were identified and sequenced...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27995313/accumulation-of-proanthocyanidins-and-or-lignin-deposition-in-buff-pigmented-soybean-seed-coats-may-lead-to-frequent-defective-cracking
#13
Mineo Senda, Naoya Yamaguchi, Miho Hiraoka, So Kawada, Ryota Iiyoshi, Kazuki Yamashita, Tomonori Sonoki, Hayato Maeda, Michio Kawasaki
Defective cracking frequently occurs in buff-pigmented soybean seed coats, where proanthocyanidins accumulate and lignin is deposited, suggesting that proanthocyanidins and/or lignin may change physical properties and lead to defective cracking. In the seed production of many yellow soybean (Glycine max) cultivars, very low percentages of self-pigmented seeds are commonly found. This phenomenon is derived from a recessive mutation of the I gene inhibiting seed coat pigmentation. In Japan, most of these self-pigmented seeds are buff-colored, and frequently show multiple defective cracks in the seed coat...
December 19, 2016: Planta
https://www.readbyqxmd.com/read/27994318/resin-bond-strength-to-water-versus-ethanol-saturated-human-dentin-pretreated-with-three-different-cross-linking-agents
#14
Bhuvan Shome Venigalla, Pinnamreddy Jyothi, Shekhar Kamishetty, Smitha Reddy, Ravi Chandra Cherukupalli, Depa Arun Reddy
CONTEXT: Resin-dentin bonds are unstable owing to hydrolytic and enzymatic degradation. Several approaches such as collagen cross-linking and ethanol-wet bonding (EWB) have been developed to overcome this problem. Collagen cross-linking improves the intrinsic properties of the collagen matrix. However, it leaves a water-rich collagen matrix with incomplete resin infiltration making it susceptible to fatigue degradation. Since EWB is expected to overcome the drawbacks of water-wet bonding (WWB), a combination of collagen cross-linking with EWB was tested...
November 2016: Journal of Conservative Dentistry: JCD
https://www.readbyqxmd.com/read/27979177/identification-of-phenolic-antioxidants-and-bioactives-of-pomegranate-seeds-following-juice-extraction-using-hplc-dad-esi-ms-n
#15
Priyatharini Ambigaipalan, Adriano Costa de Camargo, Fereidoon Shahidi
Phenolics from free and hydrolyzed fractions of pomegranate juice (PJ) and seeds (PS) were evaluated. In general, total phenolic contents and scavenging of ABTS(+), DPPH and hydroxyl radicals, as well as metal chelation of the soluble fraction from PS, were higher than those for PJ. Insoluble-bound phenolics from PS accounted for up to 27% of total scavenging capacity (free+esterified+insoluble-bound). Phenolic acids (13), monomeric flavonoids (8), hydrolysable tannins (12), proanthocyanidin (1) and anthocyanins (12) were tentatively characterized using HPLC-DAD-ESI-MS(n)...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27976241/the-neuroprotective-activity-of-a-proanthocyanidin-enriched-ginkgo-biloba-l-leaves-extract-in-vitro-and-in-vivo
#16
K Kuchta, H X Qiao, H B Huang, L Fang, Y Chen, R W Wang
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27976147/a-type-proanthocyanidins-selectively-target-acute-myeloid-leukemia-cells-in-vitro-and-in-vivo
#17
L M Bystrom, H Zong, Lal Martinez, C Neto, G J Roboz, S Rivella, M L Guzman
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27964718/dhurrin-metabolism-in-the-developing-grain-of-sorghum-bicolor-l-moench-investigated-by-metabolite-profiling-and-novel-clustering-analyses-of-time-resolved-transcriptomic-data
#18
Lasse Janniche Nielsen, Peter Stuart, Martina Pičmanová, Simon Rasmussen, Carl Erik Olsen, Jesper Harholt, Birger Lindberg Møller, Nanna Bjarnholt
BACKGROUND: The important cereal crop Sorghum bicolor (L.) Moench biosynthesize and accumulate the defensive compound dhurrin during development. Previous work has suggested multiple roles for the compound including a function as nitrogen storage/buffer. Crucial for this function is the endogenous turnover of dhurrin for which putative pathways have been suggested but not confirmed. RESULTS: In this study, the biosynthesis and endogenous turnover of dhurrin in the developing sorghum grain was studied by metabolite profiling and time-resolved transcriptome analyses...
December 13, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27960281/drought-effects-on-proanthocyanidins-in-sainfoin-onobrychis-viciifolia-scop-are-dependent-on-the-plant-s-ontogenetic-stage
#19
Carsten S Malisch, Juha-Pekka Salminen, Roland Kölliker, Marica T Engström, Daniel Suter, Bruno Studer, Andreas Lüscher
Sainfoin (Onobrychis viciifolia Scop.) is a forage legume, which improves animal health and the environmental impact of livestock farming due to its proanthocyanidin content. To identify the impact of drought on acetone/water-extractable proanthocyanidin (PA) concentration and composition in the generative and vegetative stages, a rain exclosure experiment was established. Leaves of 120 plants from 5 different sainfoin accessions were sampled repeatedly and analyzed by UPLC-ESI-MS/MS. The results showed distinct differences in response to drought between vegetative and generative plants...
December 14, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27960275/phenolics-of-selected-cranberry-genotypes-vaccinium-macrocarpon-ait-and-their-antioxidant-efficacy
#20
Gihan Abeywickrama, Samir C Debnath, Priyatharini Ambigaipalan, Fereidoon Shahidi
Free, esterified, and bound phenolic fractions of berries from five different cranberry genotypes and two market samples were evaluated for their total phenolic, flavonoid, and monomeric anthocyanin contents as well as their antioxidant efficacy using TEAC, ORAC, DPPH radical, reducing power, and ferrous ion chelation capacity assays. HPLC-MS/MS analysis was performed for two of the rich sources (Pilgrim and wild clone NL2) of phenolics and high antioxidant activity. Among the genotypes, Pilgrim showed the highest phenolic and flavonoid contents and wild clones NL3 and NL2 showed the highest monomeric anthocyanin and proanthocyanidin content, respectively...
December 14, 2016: Journal of Agricultural and Food Chemistry
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