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https://www.readbyqxmd.com/read/29778081/molecular-aspect-of-silver-nanoparticles-regulated-embryonic-development-in-zebrafish-danio-rerio-by-oct-4-expression
#1
Biplab Sarkar, Suresh K Verma, Javed Akhtar, Surya Prakash Netam, Sanjay Kr Gupta, Pritam Kumar Panda, Koel Mukherjee
With the enhancement of commercial manifestation of silver nanoparticles, concerned has risen on their accumulation in aquatic system and consequent effects on fish development and metabolism. In this study, experiments were conducted to assess the impacts of silver nanoparticles on early life cycles of fish considering Zebrafish (Danio rerio) as experimental model. Silver nanoparticles were synthesized through chemical reduction method and characterized through UV-visible spectroscopy, dynamic light scattering (DLS), and HR-TEM...
May 4, 2018: Chemosphere
https://www.readbyqxmd.com/read/29707900/an-observational-case-series-of-spinal-cord-stimulation-waveforms-visualized-on-intraoperative-neuromonitoring
#2
Steven M Falowski
INTRODUCTION: Intraoperative neuromonitoring for spinal cord stimulation uses electromyography (EMG) responses to determine myotomal coverage as a marker for dermatomal coverage. These responses are utilized to determine the orientation of the electrode. Somatosensory evoked potential (SSEP) collision testing can also be used in which sensory signals are decreased and/or eliminated when stimulation is activated. METHODS: Fifteen patients were observed in the study...
April 29, 2018: Neuromodulation: Journal of the International Neuromodulation Society
https://www.readbyqxmd.com/read/29705168/stenosis-and-neurological-level-discrepancies-in-cervical-spondylotic-myelopathy
#3
Young-Ah Choi, Keewon Kim
BACKGROUND: Cervical spondylotic myelopathy (CSM) is the most common cause of cord dysfunction in older individuals. CSM involves either the upper motor neuron or lower motor neuron (LMN) in the cervical spine. The neurological level of the LMN lesion does not usually correspond with the structural level detected via magnetic resonance imaging (MRI) OBJECTIVE: To examine the relationship between the structural level of stenosis and the neurological level of LMN involvement in patients with CSM DESIGN: Retrospective descriptive study SETTING: Tertiary hospital PATIENTS: Patients with cervical spondylotic myelopathy, aged 41 to 79 years METHODS: We reviewed electromyography (EMG) and magnetic resonance imaging (MRI) findings from 17 patients with CSM to objectively show the relationship between the structural level of stenosis and the neurological level with lower motor neuron involvement...
April 26, 2018: PM & R: the Journal of Injury, Function, and Rehabilitation
https://www.readbyqxmd.com/read/29667095/can-postoperative-deltoid-weakness-after-cervical-laminoplasty-be-prevented-by-using-intraoperative-neurophysiological-monitoring
#4
Muneharu Ando, Tetsuya Tamaki, Takuji Matsumoto, Kazuhiro Maio, Masatoshi Teraguchi, Noboru Takiguchi, Hiroki Iwahashi, Makiko Onishi, Yukihiro Nakagawa, Hiroshi Iwasaki, Shunji Tsutsui, Masanari Takami, Hiroshi Yamada
Laminoplasty, frequently performed in patients with cervical myelopathy, is safe and provides relatively good results. However, motor palsy of the upper extremities, which occurs after decompression surgery for cervical myelopathy, often reduces muscle strength of the deltoid muscle, mainly in the C5 myotome. The aim of this study was to investigate prospectively whether postoperative deltoid weakness (DW) can be predicted by performing intraoperative neurophysiological monitoring (IONM) during cervical laminoplasty and to clarify whether it is possible to prevent palsy using IONM...
April 17, 2018: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/29652243/dorsal-rhizotomy-for-children-with-spastic-diplegia-of-cerebral-palsy-origin-usefulness-of-intraoperative-monitoring
#5
George Georgoulis, Andrei Brînzeu, Marc Sindou
OBJECTIVE The utility of intraoperative neuromonitoring (ION), namely the study of muscle responses to radicular stimulation, remains controversial. The authors performed a prospective study combining ventral root (VR) stimulation for mapping anatomical levels and dorsal root (DR) stimulation as physiological testing of metameric excitability. The purpose was to evaluate to what extent the intraoperative data led to modifications in the initial decisions for surgical sectioning established by the pediatric multidisciplinary team (i...
April 13, 2018: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/29624170/segmentation-of-the-zebrafish-axial-skeleton-relies-on-notochord-sheath-cells-and-not-on-the-segmentation-clock
#6
Laura LLeras Forero, Rachna Narayanan, Leonie F A Huitema, Maaike VanBergen, Alexander Apschner, Josi Peterson-Maduro, Ive Logister, Guillaume Valentin, Luis G Morelli, Andrew Oates, Stefan Schulte-Merker
Segmentation of the axial skeleton in amniotes depends on the segmentation clock which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation...
April 6, 2018: ELife
https://www.readbyqxmd.com/read/29545250/the-importance-of-flexion-mri-in-hirayama-disease-with-special-reference-to-laminodural-space-measurements
#7
D K Boruah, A Prakash, B B Gogoi, R R Yadav, D D Dhingani, B Sarma
BACKGROUND AND PURPOSE: Hirayama disease is a benign focal amyotrophy of the distal upper limbs involving C7, C8, and T1 segmental myotomes with sparing of the brachioradialis and proximal muscles of the upper limb innervated by C5-6 myotomes. The objective of the present study was to study the utility of MR imaging in young patients presenting with weakness and wasting of the distal upper extremity and to evaluate the importance of the laminodural space during flexion cervical MR imaging...
May 2018: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/29499019/differences-in-the-transcranial-motor-evoked-potentials-between-proximal-and-distal-lower-extremity-muscles
#8
Pooja Parikh, Justin Cheongsiatmoy, Parastou Shilian, Andres A Gonzalez
PURPOSE: Transcranial motor evoked potentials (TcMEPs) are the preferred modality to monitor the integrity of motor pathways during surgery. Recently, it has also been used as a method to help with detection of nerve roots injuries. Adequate baseline muscle responses are vital to detect nerve injury. We have observed that TcMEP responses are not homogeneous across multiple myotomes, but this has not been studied systematically. Our objective is to determine whether there are any relative differences in amplitude or morphology of TcMEPs across various lower extremity muscles...
March 2018: Journal of Clinical Neurophysiology: Official Publication of the American Electroencephalographic Society
https://www.readbyqxmd.com/read/29473813/tubular-approach-to-minimally-invasive-microdiscectomy-for-pediatric-lumbar-disc-herniation
#9
Julio D Montejo, Joaquin Q Camara-Quintana, Daniel Duran, Jeannine M Rockefeller, Sierra B Conine, Alyssa M Blaise, Kristopher T Kahle, Michael L DiLuna
OBJECTIVE Lumbar disc herniation (LDH) in the pediatric population is rare and exhibits unique characteristics compared with adult LDH. There are limited data regarding the safety and efficacy of minimally invasive surgery (MIS) using tubular retractors in pediatric patients with LDH. Here, the outcomes of MIS tubular microdiscectomy for the treatment of pediatric LDH are evaluated. METHODS Twelve consecutive pediatric patients with LDH were treated with MIS tubular microdiscectomy at the authors' institution between July 2011 and October 2015...
May 2018: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/29459170/treatment-of-cervical-spondylotic-amyotrophy-with-nerve-transfers
#10
Charmaine Baxter, Thomas A Miller, Douglas C Ross, Christopher Doherty
Cervical spondylotic amyotrophy is characterized by severe, proximal upper extremity weakness including an inability to abduct the shoulder and flex the elbow. Treatment using both medical and surgical decompression approaches has produced variable results. This paper reports the use of nerve transfers (spinal accessory to suprascapular, flexor carpi ulnaris fascicle of ulnar to biceps motor branch, radial nerve branch to triceps to axillary) to restore shoulder and elbow function in a case of unilateral cervical spondylotic amyotrophy involving C5 and C6 myotomes...
February 16, 2018: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29455410/can-patients-with-symptomatic-tarlov-cysts-be-differentiated-from-patients-with-specific-low-back-pain-based-on-comprehensive-history-taking
#11
Maria Anna Hulens, Wim Dankaerts, Ricky Rasschaert, Frans Bruyninckx, Marie-Laure Willaert, Charlotte Vereecke, Greet Vansant
BACKGROUND: Tarlov cysts (TCs) are expanded nerve root sheaths that occur near the dorsal root ganglion and result from increased intraspinal hydrostatic pressure. TCs most frequently affect the lumbosacral plexus and therefore may cause specific symptoms such as perineal pain and neurogenic bladder, bowel, and sphincter problems. It has been estimated that 1% of the population has symptomatic Tarlov cysts (STCs). However, STCs appear to be underdiagnosed, with the pain reported by patients commonly attributed to degenerative alterations seen on MRI...
April 2018: Acta Neurochirurgica
https://www.readbyqxmd.com/read/29445517/autonomic-components-of-complex-regional-pain-syndrome-crps-are-favourably-affected-by-electrical-twitch-obtaining-intramuscular-stimulation-etoims-effects-on-blood-pressure-and-heart-rate
#12
Jennifer Chu, Frans Bruyninckx, Duncan V Neuhauser
Introduction: Favourable pain relief results on evoking autonomous twitches at myofascial trigger points with Electrical Twitch Obtaining Intramuscular Stimulation (ETOIMS). Aim: To document autonomic nervous system (ANS) dysfunction in Complex Regional Pain Syndrome (CRPS) from blood pressure (BP) and pulse/heart rate changes with ETOIMS. Methods and materials: A patient with persistent pain regularly received serial ETOIMS sessions of 60, 90, 120 or ≥150 min over 24 months...
July 2017: BMJ Innovations
https://www.readbyqxmd.com/read/29370293/gene-expression-of-the-two-developmentally-regulated-dermatan-sulfate-epimerases-in-the-xenopus-embryo
#13
Nadège Gouignard, Tanja Schön, Christian Holmgren, Ina Strate, Emirhan Taşöz, Franziska Wetzel, Marco Maccarana, Edgar M Pera
Chondroitin sulfate (CS)/dermatan sulfate (DS) proteoglycans are abundant on the cell surface and in the extracellular matrix and have important functions in matrix structure, cell-matrix interaction and signaling. The DS epimerases 1 and 2, encoded by Dse and Dsel, respectively, convert CS to a CS/DS hybrid chain, which is structurally and conformationally richer than CS, favouring interaction with matrix proteins and growth factors. We recently showed that Xenopus Dse is essential for the migration of neural crest cells by allowing cell surface CS/DS proteoglycans to adhere to fibronectin...
2018: PloS One
https://www.readbyqxmd.com/read/29350268/the-epitope-mediated-mmp-activation-assay-detection-and-quantification-of-the-activation-of-mmp2-in-vivo-in-the-zebrafish-embryo
#14
Emma J Jeffrey, Bryan D Crawford
Matrix remodeling is a consequence of tightly regulated matrix metalloproteinase (MMP) activity. MMPs are synthesized as inactive precursors with auto-inhibitory N-terminal propeptides, the proteolytic removal of which exposes the catalytic zinc ion, rendering the protease active. The regulation of MMP activation has been investigated primarily in tissue culture and biochemical assays that lack important biological context. Here we present the epitope-mediated MMP activation (EMMA) assay and use it to observe the activation of Mmp2 (gelatinase A) by endogenous mechanisms in the intact zebrafish embryo...
March 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29350264/linking-muscle-metabolism-and-functional-variation-to-field-swimming-performance-in-bluegill-sunfish-lepomis-macrochirus
#15
David J Ellerby, Shauna Cyr, Angela X Han, Mika Lin, Lloyd A Trueblood
Skeletal muscle has diverse mechanical roles during locomotion. In swimming fish, power-producing muscles work in concert with the accessory muscles of the fins which augment and control power transfer to the water. Although fin muscles represent a significant proportion of the locomotor muscle mass, their physiological properties are poorly characterized. To examine the relationship between muscle metabolism and the differing mechanical demands placed on distinct muscle groups, we quantified the aerobic and glycolytic capacities of the myotomal, pectoral and caudal muscles of bluegill sunfish...
January 19, 2018: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/29349631/promoter-analysis-of-the-fish-gene-of-slow-cardiac-type-myosin-heavy-chain-implicated-in-specification-of-muscle-fiber-types
#16
Shigeharu Kinoshita, Saltuk Bugrahan Ceyhun, Asaduzzamann Md, Bhuiyan Sharmin Siddique, Dadasaheb B Akolkar, Shuichi Asakawa, Shugo Watabe
Vertebrate skeletal muscles consist of heterogeneous tissues containing various types of muscle fibers, where specification of the fiber type is crucial for muscle development. Fish are an attractive experimental model to study the mechanisms of such fiber type specification because of the separated localization of slow and fast muscles in the trunk myotome. We examined regulation of expression of the torafugu gene of slow/cardiac-type myosin heavy chain, MYH M5 , and isolated an operational promoter in order to force its tissue-specific expression across different fish species via the transgenic approach in zebrafish and medaka...
April 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29204007/how-long-does-denervation-take-in-poliomyelitis-or-is-it-a-lifetime
#17
Mehmet Güney Şenol, Cengiz Kaplan, Fatih Ozdağ, Mehmet Saraçoğlu
Background and Objective: This study aims to determine the period of reinnervation in patients with poliomyelitis. This research was conducted to identify the appearance of denervation potentials in patients with poliomyelitis as indicators for reinnervation. Materials and Methods: A total of 246 male patients with poliomyelitis were assessed electrophysiologically between 1988 and 2007. The mean age was 22.8 (18-42). It has been an average of 19.9 ± 4.9 years since the beginning of complaints from the patients...
October 2017: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/29201928/abnormal-pain-response-after-a-compensable-shoulder-injury
#18
Helen Razmjou, Dragana Boljanovic, Amr Elmaraghy, Iona Macritchie, Carolyn Roknic, Danielle Medeiros, Robin R Richards
Background: The role of psychosocial factors has been established in patients with shoulder abnormalities. However, the prevalence of exaggerated pain behaviors and their association with the characteristics of injured workers have not been well studied. Purpose: To examine the prevalence of abnormal pain responses (APRs) in workers with active workers' compensation claims for a shoulder injury and to examine the differences between workers with APRs versus workers without APRs...
November 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29184116/proteomic-and-microrna-transcriptome-analysis-revealed-the-microrna-smyd1-network-regulation-in-skeletal-muscle-fibers-performance-of-chinese-perch
#19
WuYing Chu, FangLiang Zhang, Rui Song, YuLong Li, Ping Wu, Lin Chen, Jia Cheng, ShaoJun Du, JianShe Zhang
Fish myotomes are comprised of anatomically segregated fast and slow muscle fibers that possess different metabolic and contractile properties. Although the expression profile properties in fast and slow muscle fibers had been investigated at the mRNA levels, a comprehensive analysis at proteomic and microRNA transcriptomic levels is limited. In the present study, we first systematically compared the proteomic and microRNA transcriptome of the slow and fast muscles of Chinese perch (Siniperca chuatsi). Total of 2102 proteins were identified in muscle tissues...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29180480/motor-module-activation-sequence-and-topography-in-the-spinal-cord-during-air-stepping-in-human-insights-into-the-traveling-wave-in-spinal-locomotor-circuits
#20
Hikaru Yokoyama, Kohtaroh Hagio, Tetsuya Ogawa, Kimitaka Nakazawa
Coordinated locomotor muscle activity is generated by the spinal central pattern generators (CPGs), which are modulated by peripheral and supraspinal inputs. The CPGs would consist of multiple motor modules generating basic muscle activity, which are distributed rostrocaudally along the spinal cord. To activate the motor modules in proper sequence, rostrocaudally traveling waves of activation in the spinal cord are important mechanisms in the CPGs. The traveling waves of activation have been observed in nonhuman vertebrates...
November 2017: Physiological Reports
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