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https://www.readbyqxmd.com/read/29792901/identification-and-functional-characterization-including-cytokine-production-modulation-of-the-novel-chicken-interleukin-11
#1
Anh Duc Truong, Yeojin Hong, Deivendran Rengaraj, Janggeun Lee, Kyungbaek Lee, Yeong Ho Hong
Interleukin (IL)-11 plays an important role in the immune system. However, IL-11 has not yet been characterized in avian species, including chickens. This study is the first to clone and functionally characterize chicken IL-11 (chIL-11). Multiple alignments and phylogenetic tree comparisons of chIL-11 with IL-11 proteins from other species revealed high levels of conservation and a close relationship between chicken and Japanese quail IL-11. Our results demonstrate that chIL-11 was a functional ligand of IL-11RA and IL-6ST in chicken HD11 and OU2 cell lines, as well as activated and regulated JAK-STAT, NF-κB, PI3K/AKT, and MAPK signaling pathways in chicken cell lines...
May 21, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29790294/jak-stat-blockade-alters-synovial-bioenergetics-mitochondrial-function-and-pro-inflammatory-mediators-in-rheumatoid-arthritis
#2
Trudy McGarry, Carl Orr, Sarah Wade, Monika Biniecka, Siobhan Wade, Lorna Gallagher, Candice Low, Douglas J Veale, Ursula Fearon
OBJECTIVE: To examine the effect of tofacitinib on metabolic activity, mitochondrial function and pro-inflammatory mechanisms in Rheumatoid Arthritis. METHODS: Ex-vivo RA synovial explants and primary RA synovial fibroblasts (RAFLS) were cultured with tofacitinib (1μM). RAFLS bioenergetics was assessed using the XF24-Flux-analyser, and key metabolic genes by real-time PCR. Mitochondrial function was assessed using specific cell fluorescent probes and by mitochondrial gene arrays...
May 23, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29789419/detection-of-bladder-cancer-via-microfluidic-immunoassay-and-single-cell-dna-copy-number-alteration-analysis-of-captured-urinary-exfoliated-tumor-cells
#3
Anqi Chen, Guanghou Fu, Zhijie Xu, Yukun Sun, Xiaoyi Chen, Kok Suen Cheng, Kuang Hong Neoh, Zhewen Tang, Shifu Chen, Ming Liu, Tanxiao Huang, Yun Dai, Qibo Wang, Jing Jin, Baiye Jin, Ray P S Han
The increasing incidence of bladder cancer (BC) and its high rate of recurrence over a 5-year period necessitate the need for diagnosis and surveillance amelioration. Cystoscopy and urinary cytology are the current tools, and molecular techniques such as BTA stat, NMP22, survivin mRNA, and urovysion FISH have attracted attention, however, they suf-fer from insufficient sensitivity or specificity. We developed a novel microfluidic approach for harvesting intact urinary-exfoliated tumor cells (UETCs), either individually or in clus-ters, in a clean and segregated environment, which is crucial to minimize cross-contamination and misreads...
May 22, 2018: Cancer Research
https://www.readbyqxmd.com/read/29787988/microrna-30e-promotes-hepatocyte-proliferation-and-inhibits-apoptosis-in-cecal-ligation-and-puncture-induced-sepsis-through-the-jak-stat-signaling-pathway-by-binding-to-fosl2
#4
Lan Ling, Shan-Hong Zhang, Li-Da Zhi, Hong Li, Qian-Kuan Wen, Gang Li, Wen-Jia Zhang
INTRODUCTION: Hepatocyte proliferation and apoptosis are critical cellular behaviors in rat liver as a result of a liver injury. Herein, we performed this study in order to evaluate the role of miR-30e and its target Fos-Related Antigen-2 (FOSL2) in septic rats through the JAK/STAT signaling pathway. METHODS: Rat models of sepsis were induced by cecal ligation and puncture. Enzyme-linked immunosorbent assay (ELISA) was performed to access serum levels of lipopolysaccharide (LPS), inflammatory factors, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) to confirm the successful establishment of the model...
May 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29785143/pilot-study-of-the-antifibrotic-effects-of-the-multikinase-inhibitor-pacritinib-in-a-mouse-model-of-liver-fibrosis
#5
Suliman Al-Fayoumi, Taishi Hashiguchi, Yuka Shirakata, John Mascarenhas, Jack W Singer
Background: Fibrotic diseases result from an exuberant response to chronic inflammation. Myelofibrosis is the end result of inflammation in bone, caused by an inflammatory process triggered by production of abnormal myeloid cells driven by mutations affecting the JAK-STAT pathway. Inflammatory cytokine overproduction leads to increased mesenchymal cell proliferation, culminating in fibrosis. Although JAK2 inhibitors, such as the JAK1/2 inhibitor ruxolitinib and the JAK2/FLT3/CSF1R/IRAK1 inhibitor pacritinib suppress abnormal clone expansion in myelofibrosis, ruxolitinib does not appear to prevent or reverse bone-marrow fibrosis in most patients...
2018: Journal of Experimental Pharmacology
https://www.readbyqxmd.com/read/29782266/-the-association-of-gene-polymorphisms-of-matrix-metalloproteinases-9-12-and-20-and-collagen-type-i-degradation-products-with-the-remodeling-of-the-left-ventricle-in-patients-with-acute-myocardial-infarction
#6
N A Sokolova, M S Danshova, A V Govorin, D N Zaytsev, Y N Smolyakov
OBJECTIVE: to study associations of polymorphism of the genes MMP9 rs17576, MMP12 rs2276109, MMP20 rs2245803, COL1A1 rs1800012, COL1A1 rs1107946 with remodeling of the left ventricle in patients with acute myocardial infarction. MATERIALS AND METHODS: We examined 84 patients with myocardial infarction (MI). Type of early postinfarction left ventricular (LV) remodeling was determined by echocardiography on the third day of the disease. Concentration of matrix metalloproteinases (MMP) was measured by enzyme immunoassay using commercial kits; content of C-terminal telopeptide of collagen type I degradation products (ICTP) was measured by chemiluminescent immunoassay...
March 2018: Kardiologiia
https://www.readbyqxmd.com/read/29781749/oncogenic-pkc-%C3%AE-activates-vimentin-during-epithelial-mesenchymal-transition-in-melanoma-a-study-based-on-pkc-%C3%AE-and-pkc-%C3%AE-specific-inhibitors
#7
Wishrawana S Ratnayake, Christopher A Apostolatos, André H Apostolatos, Ryan J Schutte, Monica A Huynh, David A Ostrov, Mildred Acevedo-Duncan
Melanoma is one of the fastest growing cancers in the United States and is accompanied with a poor prognosis owing to tumors being resistant to most therapies. Atypical protein kinase Cs (aPKC) are involved in malignancy in many cancers. We previously reported that aPKCs play a key role in melanoma's cell motility by regulating cell signaling pathways which induce epithelial-mesenchymal Transition (EMT). We tested three novel inhibitors; [4-(5-amino-4-carbamoylimidazol-1-yl)-2,3-dihydroxycyclopentyl] methyl dihydrogen phosphate (ICA-1T) along with its nucleoside analog 5-amino-1-((1R,2S,3S,4R)-2,3-dihydroxy-4-methylcyclopentyl)-1H-imidazole-4-carboxamide (ICA-1S) which are specific to protein kinase C-iota (PKC-ι) and 8-hydroxy-1,3,6-naphthalenetrisulfonic acid (ζ-Stat) which is specific to PKC-zeta (PKC-ζ) on cell proliferation, apoptosis, migration and invasion of two malignant melanoma cell lines compared to normal melanocytes...
May 21, 2018: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/29776962/shp-1-acts-as-a-tumor-suppressor-in-hepatocarcinogenesis-and-hcc-progression
#8
Liang-Zhi Wen, Kai Ding, Ze-Rui Wang, Chen-Hong Ding, Shu-Juan Lei, Jin-Pei Liu, Chuan Yin, Ping-Fang Hu, Jin Ding, Wan-Sheng Chen, Xin Zhang, Wei-Fen Xie
Src homology region 2 (SH2) domain-containing phosphatase 1 (SHP-1, also known as PTPN6), is a nonreceptor protein tyrosine phosphatase that acts as a negative regulator of inflammation. Emerging evidence indicates that SHP-1 plays a role in inhibiting the progression of hepatocellular carcinoma (HCC). However, the role of SHP-1 in hepatocarcinogenesis remains unknown. Here we find that levels of SHP-1 are significantly downregulated in human HCC tissues compared with those in noncancerous tissues (P < 0...
May 18, 2018: Cancer Research
https://www.readbyqxmd.com/read/29775946/il-9-exhibits-elevated-expression-in-osteonecrosis-of-femoral-head-patients-and-promotes-cartilage-degradation-through-activation-of-jak-stat-signaling-in-vitro
#9
Wei Geng, Wen Zhang, Jinzhu Ma
Osteonecrosis of the femoral head (ONFH) often causes severe symptoms in young people and limits the mobility of the hip joint. Interleukin-9 (IL-9) is a multi-functional inflammatory factor that participates in lumbar disk herniation and arthritis and has been reported in many studies. However, the correlation between IL-9 and ONFH is unclear. The present study aimed to determine the role of IL-9 in the pathogenetic mechanism of osteonecrosis. To assess IL-9 expression in ONFH and femoral neck fracture patients, cartilage tissue was examined through western blot analysis and immunohistochemistry...
May 15, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29774100/differential-proteomic-profile-of-leukemic-cd34-progenitor-cells-from-chronic-myeloid-leukemia-patients
#10
Maria Rosaria Ricciardi, Valentina Salvestrini, Roberto Licchetta, Simone Mirabilii, Mattia Forcato, Gabriele Gugliotta, Simona Salati, Fausto Castagnetti, Gianantonio Rosti, Massimo Breccia, Giuliana Alimena, Rossella Manfredini, Silvio Bicciato, Roberto Massimo Lemoli, Agostino Tafuri
Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of quiescent cells which are to some extent resistant to tyrosine kinase inhibitors (TKIs). BCR-ABL oncogene activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, contributing to abnormal proliferation of clonal cells. From this perspective, the aim of this study was to analyze the expression and activation profile of STP involved in the mechanisms of cell proliferation/quiescence and survival of the progenitor CD34+ cells from chronic phase (CP) CML...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29773813/amelioration-of-experimental-autoimmune-encephalomyelitis-and-dss-induced-colitis-by-ntg-a-009-through-the-inhibition-of-th1-and-th17-cells-differentiation
#11
Suman Acharya, Maheshwor Timilshina, Liyuan Jiang, Sabita Neupane, Dong-Young Choi, Sang Won Park, Sang Yeul Lee, Byeong-Seon Jeong, Jung-Ae Kim, Tae-Gyu Nam, Jae-Hoon Chang
CD4+ T cells are the central for the mammalian adaptive immune system. Naïve CD4+ T cells mainly differentiate in to pro-inflammatory Th1, Th2 and Th17 cells upon antigenic stimulation. IFN-γ secreting Th1 cells and IL-17 secreting Th17 cells are found to play key roles in autoimmune diseases like multiple sclerosis (MS) and ulcerative colitis (UC). In this study we found NTG-A-009, 6-aminopyridin-3-ol, has great inhibitory effect on in vitro differentiation of Th1 and Th17 cells without affecting regulatory T cells...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29772037/a-review-of-fabry-disease
#12
A Cinats, E Heck
The class of medications known as Janus kinase inhibitors block cytokine-mediated signaling via the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway, which plays an important role in immunoregulation and normal cell growth. This class includes the drugs tofacitinib, approved for the treatment of rheumatoid arthritis, and ruxolitinib, approved for the treatment of myelofibrosis and polycythemia rubra vera. The most common adverse events (AEs) reported in patients taking tofacitinib are infections, whereas the most common AEs in patients taking ruxolitinib are anemia and thrombocytopenia...
May 2018: Skin Therapy Letter
https://www.readbyqxmd.com/read/29771047/-effect-of-shrna-targeting-silencing-of-vtcn1-gene-on-nasopharyngeal-carcinoma-cells
#13
J Dong, T Peng, P Song, S C Yin, X H Zhou
Objective: The aim of this study is to investigate the expression of VTCN1 in nasopharyngeal carcinoma cells and targeting silencing of VTCN1 gene by infected with lentiviral vector to inhibit the proliferation and invasion in nasopharyngeal carcinoma cells. Method: The VTCN1 expression level of different nasopharyngeal carcinoma cells was detected by RT-PCR and Western blotting, the cell lines of the most expression level were seleted to conduct the Subsequent experiments; The lentiviral vector of silenced VTCN1 was transfected into HNE2 cells with VTCN1 expression by lipofectamine 2000, and stable cell lines were screened...
May 20, 2017: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
https://www.readbyqxmd.com/read/29770454/streamlining-a-blood-center-and-hospital-transfusion-service-supply-chain-with-an-informatics-vendor-managed-inventory-solution-development-implementation-and-3-month-follow-up
#14
Hamilton C Tsang, Adam Garcia, Robert Scott, David Lancaster, Dianne Geary, Anh-Thu Nguyen, Raina Shankar, Leslie Buchanan, Tho D Pham
BACKGROUND: The ordering process at Stanford Health Care involved twice-daily shipments predicated upon current stock levels from the blood center to the hospital transfusion service. Manual census determination is time consuming and error prone. We aimed to enhance inventory management by developing an informatics platform to streamline the ordering process and reallocate staff productivity. STUDY DESIGN AND METHODS: The general inventory accounts for more than 50 product categories based on characteristics including component, blood type, irradiation status, and cytomegalovirus serology status...
May 16, 2018: Transfusion
https://www.readbyqxmd.com/read/29769165/radial-hemostasis-is-facilitated-with-a-potassium-ferrate-hemostatic-patch-the-statseal-with-tr-band-assessment-trial-stat
#15
Arnold H Seto, William Rollefson, Mitul P Patel, William M Suh, David M Tehrani, Jacqueline A Nguyen, Daniel G Amador, Omid Behnamfar, Vinisha Garg, Mauricio G Cohen
AIMS: Hemostasis is a limiting factor for discharge after uncomplicated transradial procedures. The potassium ferrate hemostatic patch (PFHP) may serve as an adjunct to the air-bladder TR band (TRB) and allow expedited deflation of the TRB. METHODS AND RESULTS: Prospective multicenter randomized controlled trial comparing radial hemostatic protocols. Deflation of the TRB was attempted at 40 minutes with PFHP and at 120 minutes without the PFHP. The primary outcome was time to full deflation of the TRB with hemostasis...
May 15, 2018: EuroIntervention
https://www.readbyqxmd.com/read/29765954/an-unusual-presentation-of-esthesioneuroblastoma-in-a-young-pregnant-female
#16
Meenakshi Wadhwani, Amar Ranjan, Kulbhushan Gangwani, Prasenjit Das
Background: Esthesioneuroblastoma is a rare neuroepithelial tumor arising from the olfactory epithelium in the cribriform plate or nasal cavity. It accounts for 1-5% of cases of malignant neoplasms of the nasal cavity. Methods: A 20-year-old pregnant female presented with painful loss of vision in both eyes, on imaging diagnosed as meningioma. There was a progressive increase in pain along with diminution of vision to the extent of complete loss of vision in the right eye...
April 2018: Ocular Oncology and Pathology
https://www.readbyqxmd.com/read/29765906/epstein-barr-virus-and-human-papillomaviruses-interactions-and-their-roles-in-the-initiation-of-epithelial-mesenchymal-transition-and-cancer-progression
#17
REVIEW
Farhan S Cyprian, Halema F Al-Farsi, Semir Vranic, Saghir Akhtar, Ala-Eddin Al Moustafa
Oncoviruses are implicated in around 20% of all human cancers including both solid and non-solid malignancies. Epstein-Barr virus (EBV) and human papillomaviruses (HPVs) are the most common oncoviruses worldwide. Currently, it is well established that onco-proteins of EBV (LMP1, LMP2A, and EBNA1) and high-risk HPVs (E5 and E6/E7) play an important role in the initiation and/or progression of several human carcinomas, including cervical, oral, and breast. More significantly, it has been recently pointed out that viral onco-proteins of EBV and high-risk HPVs can be co-present and consequently cooperate to initiate and/or amplify epithelial-mesenchymal transition (EMT), which is the hallmark of cancer progression and metastasis...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29765448/overexpressed-ho-1-is-associated-with-reduced-stat3-activation-in-preeclampsia-placenta-and-inhibits-stat3-phosphorylation-in-placental-jeg-3-cells-under-hypoxia
#18
Hong-Mei Qu, Li-Ping Qu, Xiao-Yan Li, Xian-Zhen Pan
Introduction: Inadequate trophoblast invasion and placentation are widely believed to contribute to preeclampsia, and multiple lines of evidence indicate the involvement of hypoxia in preeclampsia. However, the molecular mechanisms underlying the association of placental hypoxia with preeclampsia are not clear. Material and methods: The present study focused on the role in preeclampsia of heme oxygenase 1 (HO-1), which is an inducible isoform of HO in response to hypoxia, via examining the expression of HO-1 and the expression and phosphorylation (Tyr705) of Signal transducer and activator of transcription (STAT) 3 in preeclamptic placentas via the immunohistochemical method, western blotting assay and RT-qPCR method...
April 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29765155/genetic-transcriptional-and-post-translational-regulation-of-the-programmed-death-protein-ligand-1-in-cancer-biology-and-clinical-correlations
#19
REVIEW
Ioannis Zerdes, Alexios Matikas, Jonas Bergh, George Z Rassidakis, Theodoros Foukakis
The programmed death protein 1 (PD-1) and its ligand (PD-L1) represent a well-characterized immune checkpoint in cancer, effectively targeted by monoclonal antibodies that are approved for routine clinical use. The regulation of PD-L1 expression is complex, varies between different tumor types and occurs at the genetic, transcriptional and post-transcriptional levels. Copy number alterations of PD-L1 locus have been reported with varying frequency in several tumor types. At the transcriptional level, a number of transcriptional factors seem to regulate PD-L1 expression including HIF-1, STAT3, NF-κΒ, and AP-1...
May 16, 2018: Oncogene
https://www.readbyqxmd.com/read/29765118/ifn-%C3%AE-s-inhibit-hantaan-virus-infection-through-the-jak-stat-pathway-and-expression-of-mx2-protein
#20
Ning Li, Fan Luo, Qingzhou Chen, Ni Zhu, Hui Wang, Linlin Xie, Hairong Xiong, Ming Yue, Yun Zhang, Yong Feng, Wei Hou
Hantaan virus (HTNV), member of the newly defined Hantaviridae family, within the order Bunyavirales, can cause a hemorrhagic fever with renal syndrome with high fatality rates in humans. However, no specific antiviral agents are currently available for HTNV infection approved by the US Food and Drug Administration. Although interferon lambdas (IFN-λs) have been shown to induce an antiviral state against HTNV, the molecular mechanisms remain to be determined. In this study, we found that IFN-λs exerted its anti-HTNV effect by activating Janus kinase/signal transducers and activators of transcription (JAK-STAT) pathway-mediated antiviral immunity in A549 cells...
May 15, 2018: Genes and Immunity
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