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https://www.readbyqxmd.com/read/28733955/detection-of-plasma-biomarkers-using-immunomagnetic-reduction-a-promising-method-for-the-early-diagnosis-of-alzheimer-s-disease
#1
REVIEW
Shieh-Yueh Yang, Ming-Jang Chiu, Ta-Fu Chen, Herng-Er Horng
Alzheimer's disease (AD) is the most common form of dementia. The development of assay technologies able to diagnose early-stage AD is important. Blood tests to detect biomarkers, such as amyloid and total Tau protein, are among the most promising diagnostic methods due to their low cost, low risk, and ease of operation. However, such biomarkers in blood occur at extremely low levels and are difficult to detect precisely. In the early 2000s, a highly sensitive assay technology, immunomagnetic reduction (IMR), was developed...
July 2017: Neurology and Therapy
https://www.readbyqxmd.com/read/28522341/systemic-and-ocular-fluid-compounds-as-potential-biomarkers-in-age-related-macular-degeneration
#2
REVIEW
Eveline Kersten, Constantin C Paun, Rosa L Schellevis, Carel B Hoyng, Cécile Delcourt, Imre Lengyel, Tunde Peto, Marius Ueffing, Caroline C W Klaver, Sascha Dammeier, Anneke I den Hollander, Eiko K de Jong
Biomarkers can help unravel mechanisms of disease and identify new targets for therapy. They can also be useful in clinical practice for monitoring disease progression, evaluation of treatment efficacy and risk assessment in multifactorial diseases, such as age-related macular degeneration (AMD). AMD is a highly prevalent progressive retinal disorder for which multiple genetic and environmental risk factors have been described, but the exact etiology is not yet fully understood. Many compounds have been evaluated for their association with AMD...
May 15, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28506937/bivalirudin-infusion-to-reduce-ventricular-infarction-the-open-label-randomised-bivalirudin-infusion-for-ventricular-infarction-limitation-bival-study
#3
Robert Jan van Geuns, Georgios Sideris, Niels Van Royen, Rami El Mahmoud, Roberto Diletti, Claire Bal Dit Sollier, Jerome Garot, Nina W Van Der Hoeven, Bernardo Cortese, Li Ding, Ilknur Lechthaler, Efthymios N Deliargyris, Prodromos Anthopoulos, Ludovic Drouet
AIMS: The aim of the study was to investigate whether bivalirudin versus unfractionated heparin (UFH) reduces infarct size (IS) for primary percutaneous coronary intervention (PPCI) in large acute myocardial infarction (AMI). METHODS AND RESULTS: This multicentre open-label trial randomised 78 patients undergoing PPCI for large AMI to bivalirudin or UFH. The primary endpoint was IS, assessed by cardiac magnetic resonance (CMR) five days after PPCI. Secondary endpoints included index of microcirculatory resistance (IMR), CMR-assessed microvascular obstruction (MVO) and ejection fraction, and biomarkers for thrombin activity and cell injury...
May 16, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28475267/sublethal-effects-of-the-pyrethroid-insecticide-deltamethrin-on-oxidative-stress-parameters-in-green-toad-bufotes-viridis-l
#4
Tijana B Radovanović, Mohammed Nasia, Imre I Krizmanić, Marko D Prokić, Jelena P Gavrić, Svetlana G Despotović, Branka R Gavrilović, Slavica S Borković-Mitić, Slađan Z Pavlović, Zorica S Saičić
Pyrethroids are synthetic insecticides widely used in agriculture, public health and veterinary medicine. Deltamethrin, a type II of pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides, including pyrethroids, in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation (LPO) and disruption of the total antioxidant potential. The aim of present study was to investigate the effects of acute toxicity of three different concentrations (8 mg/kg body weight (bw), 16 mg/kg bw and 32 mg/kg bw) of orally applied deltamethrin after 96 hours of treatment...
May 5, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28326170/obstacles-and-opportunities-in-the-functional-analysis-of-extracellular-vesicle-rna-an-isev-position-paper
#5
Bogdan Mateescu, Emma J K Kowal, Bas W M van Balkom, Sabine Bartel, Suvendra N Bhattacharyya, Edit I Buzás, Amy H Buck, Paola de Candia, Franklin W N Chow, Saumya Das, Tom A P Driedonks, Lola Fernández-Messina, Franziska Haderk, Andrew F Hill, Jennifer C Jones, Kendall R Van Keuren-Jensen, Charles P Lai, Cecilia Lässer, Italia di Liegro, Taral R Lunavat, Magdalena J Lorenowicz, Sybren L N Maas, Imre Mäger, Maria Mittelbrunn, Stefan Momma, Kamalika Mukherjee, Muhammed Nawaz, D Michiel Pegtel, Michael W Pfaffl, Raymond M Schiffelers, Hidetoshi Tahara, Clotilde Théry, Juan Pablo Tosar, Marca H M Wauben, Kenneth W Witwer, Esther N M Nolte-'t Hoen
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28248256/integrated-proteomic-and-transcriptomic-based-approaches-to-identifying-signature-biomarkers-and-pathways-for-elucidation-of-daoy-and-uw228-subtypes
#6
Roger Higdon, Jessie Kala, Devan Wilkins, Julia Fangfei Yan, Manveen K Sethi, Liang Lin, Siqi Liu, Elizabeth Montague, Imre Janko, John Choiniere, Natali Kolker, William S Hancock, Eugene Kolker, Susan Fanayan
Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Patient survival has remained largely the same for the past 20 years, with therapies causing significant health, cognitive, behavioral and developmental complications for those who survive the tumor. In this study, we profiled the total transcriptome and proteome of two established MB cell lines, Daoy and UW228, using high-throughput RNA sequencing (RNA-Seq) and label-free nano-LC-MS/MS-based quantitative proteomics, coupled with advanced pathway analysis...
February 3, 2017: Proteomes
https://www.readbyqxmd.com/read/28198381/bio-functionalized-magnetic-nanoparticles-for-the-immunoassay-of-fetal-fibronectin-a-feasibility-study-for-the-prediction-of-preterm-birth
#7
Chian-Huey Wong, Chie-Pein Chen, Chia-Chen Chang, Chen-Yu Chen
Preterm birth is an important cause of perinatal morbidity and mortality. Various biomarkers in cervicovaginal secretions related to preterm birth have been investigated, of which foetal fibronectin (fFN) shows the greatest potential because of its high negative predictive value. The immunomagnetic reduction (IMR) assay has emerged as a novel quantitative method to detect biomarkers. In this prospective case-control study, we analysed 33 samples of cervicovaginal secretions from pregnant women between 22 and 34 weeks of gestation at high risk of preterm birth...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090288/expression-profiling-elucidates-a-molecular-gene-signature-for-pulmonary-hypertension-in-sarcoidosis
#8
Sunit Singla, Tong Zhou, Kamran Javaid, Taimur Abbasi, Nancy Casanova, Wei Zhang, Shwu-Fan Ma, Michael S Wade, Imre Noth, Nadera J Sweiss, Joe G N Garcia, Roberto F Machado
Pulmonary hypertension (PH), when it complicates sarcoidosis, carries a poor prognosis, in part because it is difficult to detect early in patients with worsening respiratory symptoms. Pathogenesis of sarcoidosis occurs via incompletely characterized mechanisms that are distinct from the mechanisms of pulmonary vascular remodeling well known to occur in conjunction with other chronic lung diseases. To address the need for a biomarker to aid in early detection as well as the gap in knowledge regarding the mechanisms of PH in sarcoidosis, we used genome-wide peripheral blood gene expression analysis and identified an 18-gene signature capable of distinguishing sarcoidosis patients with PH (n = 8), sarcoidosis patients without PH (n = 17), and healthy controls (n = 45)...
December 2016: Pulmonary Circulation
https://www.readbyqxmd.com/read/28053571/comparative-study-of-oxidative-stress-parameters-and-acetylcholinesterase-activity-in-the-liver-of-pelophylax-esculentus-complex-frogs
#9
Marko Prokić, Slavica Borković-Mitić, Imre Krizmanić, Jelena Gavrić, Svetlana Despotović, Branka Gavrilović, Tijana Radovanović, Slađan Pavlović, Zorica Saičić
Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (-SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to the Pelophylax esculentus "complex" (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax kl...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/27907905/association-of-high-microvessel-%C3%AE-v%C3%AE-3-and-low-pten-with-poor-outcome-in-stage-3-neuroblastoma-rationale-for-using-first-in-class-dual-pi3k-brd4-inhibitor-sf1126
#10
Anat Erdreich-Epstein, Alok R Singh, Shweta Joshi, Francisco M Vega, Pinzheng Guo, Jingying Xu, Susan Groshen, Wei Ye, Melissa Millard, Mihaela Campan, Guillermo Morales, Joseph R Garlich, Peter W Laird, Robert C Seeger, Hiroyuki Shimada, Donald L Durden
Neuroblastoma (NB) is the most common extracranial solid tumor in children. Our previous studies showed that the angiogenic integrin αvβ3 was increased in high-risk metastatic (stage 4) NB compared with localized neuroblastomas. Herein, we show that integrin αvβ3 was expressed on 68% of microvessels in MYCN-amplified stage 3 neuroblastomas, but only on 34% (means) in MYCN-non-amplified tumors (p < 0.001; n = 54). PTEN, a tumor suppressor involved in αvβ3 signaling, was expressed in neuroblastomas either diffusely, focally or not at all (immunohistochemistry)...
November 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27629268/bioaccumulation-and-effects-of-metals-on-oxidative-stress-and-neurotoxicity-parameters-in-the-frogs-from-the-pelophylax-esculentus-complex
#11
Marko D Prokić, Slavica S Borković-Mitić, Imre I Krizmanić, Jelena J Mutić, Jelena Đ Trifković, Jelena P Gavrić, Svetlana G Despotović, Branka R Gavrilović, Tijana B Radovanović, Slađan Z Pavlović, Zorica S Saičić
Metals are involved in the formation of reactive oxygen species and can induce oxidative stress. The aim of this study was to assess the effects of several metals on oxidative stress in the skin and muscle of the Pelophylax esculentus "complex" frogs (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in Serbia, and the potential use of these species as bioindicator organisms in biomonitoring studies. The biomarkers of oxidative stress (SOD, CAT, GSH-Px, GR, GST activities and GSH, SH concentrations) and cholinesterase activity were investigated...
October 2016: Ecotoxicology
https://www.readbyqxmd.com/read/27466195/selective-release-of-muscle-specific-extracellular-micrornas-during-myogenic-differentiation
#12
Anna M L Coenen-Stass, Corinne A Betts, Yi F Lee, Imre Mäger, Mikko P Turunen, Samir El Andaloussi, Jennifer E Morgan, Matthew J A Wood, Thomas C Roberts
MyomiRs are muscle-specific microRNAs (miRNAs) that regulate myoblast proliferation and differentiation. Extracellular myomiRs (ex-myomiRs) are highly enriched in the serum of Duchenne Muscular Dystrophy (DMD) patients and dystrophic mouse models and consequently have potential as disease biomarkers. The biological significance of miRNAs present in the extracellular space is not currently well understood. Here we demonstrate that ex-myomiR levels are elevated in perinatal muscle development, during the regenerative phase that follows exercise-induced myoinjury, and concomitant with myoblast differentiation in culture...
September 15, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/27278241/development-of-an-ultra-high-sensitive-immunoassay-with-plasma-biomarker-for-differentiating-parkinson-disease-dementia-from-parkinson-disease-using-antibody-functionalized-magnetic-nanoparticles
#13
Shieh-Yueh Yang, Ming-Jang Chiu, Chin-Hsien Lin, Herng-Er Horng, Che-Chuan Yang, Jen-Jie Chieh, Hsin-Hsien Chen, Bing-Hsien Liu
BACKGROUND: It is difficult to discriminate healthy subjects and patients with Parkinson disease (PD) or Parkinson disease dementia (PDD) by assaying plasma α-synuclein because the concentrations of circulating α-synuclein in the blood are almost the same as the low-detection limit using current immunoassays, such as enzyme-linked immunosorbent assay. In this work, an ultra-sensitive immunoassay utilizing immunomagnetic reduction (IMR) is developed. The reagent for IMR consists of magnetic nanoparticles functionalized with antibodies against α-synuclein and dispersed in pH-7...
June 8, 2016: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/27230444/emphysema-and-airway-dominant-copd-phenotypes-defined-by-standardised-quantitative-computed-tomography
#14
Deepak R Subramanian, Sumit Gupta, Dorothe Burggraf, Suzan J Vom Silberberg, Irene Heimbeck, Marion S Heiss-Neumann, Karl Haeussinger, Chris Newby, Beverley Hargadon, Vimal Raj, Dave Singh, Umme Kolsum, Thomas P Hofer, Khaled Al-Shair, Niklas Luetzen, Antje Prasse, Joachim Müller-Quernheim, Giorgio Benea, Stefano Leprotti, Piera Boschetto, Dorota Gorecka, Adam Nowinski, Karina Oniszh, Wolfgang Zu Castell, Michael Hagen, Imre Barta, Balázs Döme, Janos Strausz, Timm Greulich, Claus Vogelmeier, Andreas R Koczulla, Ivo Gut, Jens Hohlfeld, Tobias Welte, Mahyar Lavae-Mokhtari, Loems Ziegler-Heitbrock, Christopher Brightling, David G Parr
EvA (Emphysema versus Airway disease) is a multicentre project to study mechanisms and identify biomarkers of emphysema and airway disease in chronic obstructive pulmonary disease (COPD). The objective of this study was to delineate objectively imaging-based emphysema-dominant and airway disease-dominant phenotypes using quantitative computed tomography (QCT) indices, standardised with a novel phantom-based approach.441 subjects with COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) stages 1-3) were assessed in terms of clinical and physiological measurements, laboratory testing and standardised QCT indices of emphysema and airway wall geometry...
July 2016: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/27174238/extracellular-vesicles-in-neurodegenerative-disease-pathogenesis-to-biomarkers
#15
REVIEW
Alexander G Thompson, Elizabeth Gray, Sabrina M Heman-Ackah, Imre Mäger, Kevin Talbot, Samir El Andaloussi, Matthew J Wood, Martin R Turner
To develop effective disease-modifying therapies for neurodegenerative diseases, reliable markers of diagnosis, disease activity and progression are a research priority. The fact that neurodegenerative pathology is primarily associated with distinct subsets of cells in discrete areas of the CNS makes the identification of relevant biomarker molecules a challenge. The trafficking of macromolecules from the CNS to the cerebrospinal fluid and blood, mediated by extracellular vesicles (EVs), presents a promising source of CNS-specific biomarkers...
June 2016: Nature Reviews. Neurology
https://www.readbyqxmd.com/read/26846240/biomarkers-of-oxidative-stress-and-metal-accumulation-in-marsh-frog-pelophylax-ridibundus
#16
Slavica S Borković-Mitić, Marko D Prokić, Imre I Krizmanić, Jelena Mutić, Jelena Trifković, Jelena Gavrić, Svetlana G Despotović, Branka R Gavrilović, Tijana B Radovanović, Slađan Z Pavlović, Zorica S Saičić
To understand the effect of metals on the marsh frog Pelophylax ridibundus and the possible environment-induced changes in oxidative stress enzymes, we determined the concentrations of 18 metals: Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Ga, Hg, In, Li, Mn, Ni, Pb, Sr, and Zn, in the tissues (liver, skin, and muscle) and water samples collected from different locations in Serbia. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST), acetylcholinesterase (AChE), and changes in concentrations of reduced glutathione (GSH) and sulfhydryl groups (SH) were analyzed in the tissues of the sampled frogs...
May 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/26608198/extracellular-micrornas-in-membrane-vesicles-and-non-vesicular-carriers
#17
Anna M L Coenen-Stass, Imre Mäger, Mathew J A Wood
Great excitement has surrounded the finding that small RNAs are stable in various biofluids and carry specific signatures reflecting physiological and pathological states. In this chapter, we briefly describe the impact of this revolutionary discovery and introduce different subclasses of circulating microRNAs based on their mode of transport. Subsequently, we review the current state-of-the art knowledge on microRNA selection for export, secretion and possible uptake mechanisms and their potential function in circulation...
2015: EXS
https://www.readbyqxmd.com/read/26469522/identification-of-ddx39a-as-a-potential-biomarker-for-unfavorable-neuroblastoma-using-a-proteomic-approach
#18
Kohei Otake, Keiichi Uchida, Shozo Ide, Yuhko Kobayashi, Issei Kobayashi, Masato Kusunoki
BACKGROUND: Malignant potential in unfavorable neuroblastoma (NB) is dependent on an undifferentiated status. The aim of this study was to identify a novel biomarker associated with the undifferentiated status of NB in vitro and to evaluate its prognostic implication. PROCEDURE: Shotgun proteomic analysis was performed in undifferentiated and all trans-retinoic acid induced differentiated NB cells in vitro. An identified protein was verified by multiple reaction monitoring (MRM) and evaluated by Western blot analysis...
February 2016: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/26064421/endogenous-estrogen-mediated-heme-oxygenase-regulation-in-experimental-menopause
#19
Anikó Pósa, Renáta Szabó, Anett Csonka, Médea Veszelka, Anikó Magyariné Berkó, Zoltán Baráth, Rudolf Ménesi, Imre Pávó, Mariann Gyöngyösi, Ferenc László, Krisztina Kupai, Csaba Varga
Estrogen deficiency is one of the main causes of age-associated diseases in the cardiovascular system. Female Wistar rats were divided into four experimental groups: pharmacologically ovariectomized, surgically ovariectomized, and 24-month-old intact aging animals were compared with a control group. The activity and expression of heme oxygenases (HO) in the cardiac left ventricle, the concentrations of cardiac interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), the myeloperoxidase (MPO) activity in the cardiac left ventricle, and the effects of heme oxygenase blockade (by 24-hour and 1-hour pretreatment with tin-protoporphyrin IX, SnPP) on the epinephrine and phentolamine-induced electrocardiogram ST segment changes in vivo were investigated...
2015: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/26022510/serum-free-culture-alters-the-quantity-and-protein-composition-of-neuroblastoma-derived-extracellular-vesicles
#20
Jinghuan Li, Yi Lee, Henrik J Johansson, Imre Mäger, Pieter Vader, Joel Z Nordin, Oscar P B Wiklander, Janne Lehtiö, Matthew J A Wood, Samir El Andaloussi
Extracellular vesicles (EVs) play a significant role in cell-cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis and release from cells. However, little is known about how external stimuli such as cell culture conditions can affect the quantity and content of EVs. While N2a neuroblastoma cells cultured in serum-free (OptiMEM) conditions did not result in EVs with significant biophysical or size differences compared with cells cultured in serum-containing (pre-spun) conditions, the quantity of isolated EVs was greatly increased...
2015: Journal of Extracellular Vesicles
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