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https://www.readbyqxmd.com/read/29774401/high-levels-of-serum-sclerostin-and-dkk1-in-a-case-of-klippel-tr%C3%A3-naunay-syndrome
#1
P Muto, A Lo Gullo, G Mandraffino, S Loddo, M Atteritano
Klippel-Trénaunay syndrome (KTS) is described as a complex syndrome characterized by various combinations of capillary, venous, and lymphatic malformations associated with bone and soft tissue hypertrophy. We report a case of a 67-year-old postmenopausal Caucasian women with KTS that shows elevated levels of sclerostin and Dickkopf-related protein 1 (DKK1). Dual-energy X-ray absorptiometry (DXA) BMD T-scores at lumbar spine and femur were normal. Serum calcium and phosphorus levels were consistently normal, 25-hydroxyvitamin D (25OHD) < 30 ng/mL, and normal parathyroid hormone (PTH)...
May 17, 2018: Osteoporosis International
https://www.readbyqxmd.com/read/29772019/the-effect-of-abandonment-on-vegetation-composition-and-soil-properties-in-molinion-meadows-sw-poland
#2
Grzegorz Swacha, Zoltán Botta-Dukát, Zygmunt Kącki, Daniel Pruchniewicz, Ludwik Żołnierz
Intermittently wet meadows of the Molinion alliance, as with many other grasslands of high-nature value, have become increasingly exposed to abandonment due to their low economic value. The potential consequences of land abandonment are the decrease in species diversity and environmental alterations. The issue of land-use induced changes in plant species composition and soil physico-chemical parameters have been rarely studied in species-rich intermittently wet grasslands. In this study we attempt to i) to identify determinants of plant species composition patterns and ii) to investigate the effect of cessation of mowing on vegetation composition and soil properties...
2018: PloS One
https://www.readbyqxmd.com/read/29770988/probing-calcium-based-metal-organic-frameworks-via-natural-abundance-43ca-solid-state-nmr
#3
Shoushun Chen, Bryan Lucier, Mansheng Chen, Victor Terskikh, Yining Huang
Calcium-based MOFs are of high importance due to their low cost and bio-compatible metal centers. Understanding the local environment of calcium in these materials is critical for unraveling the origins of specific MOF properties. 43Ca solid-state NMR is one of the very few spectroscopic techniques that can directly characterize calcium metal centers, however, the 43Ca nucleus is a very challenging target for solid-state NMR due to its extremely low natural abundance and resonant frequency. In this work, natural abundance 43Ca solid-state NMR at a high magnetic field of 21...
May 17, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29770866/meucin-49-a-multifunctional-scorpion-venom-peptide-with-bactericidal-synergy-with-neurotoxins
#4
Bin Gao, Julie Dalziel, Simone Tanzi, Shunyi Zhu
Besides key roles in prey capture and predator defense, scorpion venom also functions as internal immune agents protecting the venom gland from infection and external immune agents cleaning saprophytic microbes from their own body surfaces. However, antimicrobials (typically antimicrobial peptides, AMPs) in the venom often exist in low abundance that might exclude their immune role alone, leaving an open question with regard to their in vivo biological function. Here, we report the bactericidal activity of seven peptides isolated from the scorpion Mesobuthus eupeus venom, including one classical α-helical AMP and five ion channel-targeted neurotoxins...
May 16, 2018: Amino Acids
https://www.readbyqxmd.com/read/29767557/polycystin-1-dysfunction-impairs-electrolyte-and-water-handling-in-a-renal-pre-cystic-mouse-model-for-adpkd
#5
Eric H J Verschuren, Sami G Mohammed, Wouter N Leonhard, Caro Overmars-Bos, Kimberly A M Veraar, Joost G J Hoenderop, René J M Bindels, Dorien J M Peters, Francisco J Arjona
The PKD1 gene encodes polycystin-1 (PC1), a mechanosensor triggering intracellular responses upon urinary flow sensing in kidney tubular cells. Mutations in PKD1 lead to autosomal dominant polycystic kidney disease (ADPKD). The involvement of PC1 in renal electrolyte handling remains unknown since renal electrolyte physiology in ADPKD patients has only been characterized in cystic ADPKD. We thus studied the renal electrolyte handling in inducible kidney-specific Pkd1 knockout (iKsp-Pkd1-/-) mice manifesting a pre-cystic phenotype...
May 16, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29766656/microencapsulated-rabbit-adipose-stem-cells-initiate-tissue-regeneration-in-a-rabbit-ear-defect-model
#6
Shirae K Leslie, David J Cohen, Sharon L Hyzy, Christopher R Dosier, Anthony Nicolini, Janina Sedlaczek, Zvi Schwartz, Barbara D Boyan
Cell-based tissue engineering can promote cartilage tissue regeneration, but cell retention in the implant site post-delivery is problematic. Alginate microbeads containing adipose stem cells (ASCs) pretreated with chondrogenic media (CM) have been used successfully to regenerate hyaline cartilage in critical size defects in rat xiphoid suggesting that they may be used to treat defects in elastic cartilages like the ear. To test this, we used microbeads made with low viscosity, high mannuronate medical grade alginate using a high electrostatic potential and a calcium cross linking solution containing glucose...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29766357/enhancement-of-calcification-by-osteoblasts-cultured-on-hydroxyapatite-surfaces-with-adsorbed-inorganic-polyphosphate
#7
Kan Kato, Koji Morita, Isao Hirata, Kazuya Doi, Takayasu Kubo, Koichi Kato, Kazuhiro Tsuga
Inorganic polyphosphate has been expected to accelerate bone regeneration. However, there are limited evidences to prove that polyphosphate adsorbed on the surface of a hydroxyapatite plate enhances calcification of cultured osteoblasts. In this study, we examined the effect of polyphosphate adsorbed onto the surface of a hydroxyapatite plate on the attachment, proliferation, differentiation, and calcification of osteoblasts. After hydroxyapatite plates were soaked in solutions of polyphosphate, the plate surfaces were analyzed by scanning electron microscopy and toluidine blue staining to confirm adsorption of polyphosphate...
May 15, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29765903/effect-of-ultrasonic-agitation-on-push-out-bond-strength-and-adaptation-of-root-end-filling-materials
#8
Murilo Priori Alcalde, Rodrigo Ricci Vivan, Marina Angélica Marciano, Jussaro Alves Duque, Samuel Lucas Fernandes, Mariana Bailo Rosseto, Marco Antonio Hungaro Duarte
Objectives: This study evaluated the effect of ultrasonic agitation of mineral trioxide aggregate (MTA), calcium silicate-based cement (CSC), and Sealer 26 (S26) on adaptation at the cement/dentin interface and push-out bond strength. Materials and Methods: Sixty maxillary canines were divided into 6 groups ( n = 10): MTA, S26, and CSC, with or without ultrasonic activation (US). After obturation, the apical portions of the teeth were sectioned, and retrograde cavities were prepared and filled with cement by hand condensation...
May 2018: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/29765146/do-dietary-calcium-and-vitamin-d-matter-in-men-with-prostate-cancer
#9
REVIEW
Thierry Capiod, Nicolas Barry Delongchamps, Natascha Pigat, Jean-Claude Souberbielle, Vincent Goffin
Active surveillance (AS) is an attractive alternative to immediate treatment for men with low-risk prostate cancer. Thus, the identification of environmental factors that promote the progression of indolent disease towards aggressive stages is critical to optimize clinical management. Epidemiological studies suggest that calcium-rich diets contribute to an increased risk of developing prostate cancer and that vitamin D reduces this risk. However, the potential effect of these nutrients on the progression of early-stage prostate tumours is uncertain, as studies in this setting are scarce and have not provided unambiguous conclusions...
May 15, 2018: Nature Reviews. Urology
https://www.readbyqxmd.com/read/29761964/-real-time-investigation-of-dynamic-morphology-of-live-platelets-and-generation-of-platelet-microparticles-using-hopping-probe-ion-conductance-microscopy
#10
Xiao Liu, Yufu Luo, Yanjun Zhang
Platelets are rapidly activated by activators and produce a large number of platelet microparticles (PMPs) with high coagulation activity, resulting in coagulation dysfunction. However, the generation mechanism of PMPs is still not clear. Hopping probe ion conductance microscopy (HPICM) has special technical advantages in non-contact, real-time, high-resolution imaging of living cells under physiological conditions. Using HPICM, this study monitored the processes of platelet activation and generation of PMPs in real time in the presence of calcium ionophore A23187 and cytochalasin D (CD), respectively...
August 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29761657/nesting-environment-may-drive-variation-in-eggshell-structure-and-egg-characteristics-in-the-testudinata
#11
D Charles Deeming
Testudines exhibit considerable variation in the degree of eggshell calcification, which affects eggshell conductance, water physiology of the embryos, and calcium metabolism of embryos. However, the underlying reason for different shell types has not been explored. Phylogenetically controlled analyses examined relationships between egg size, shell mass, and clutch size in ∼200 turtle species from a range of body sizes and assigned by family as laying either rigid- or pliable-shelled eggs. Shell type affected egg breadth relative to pelvic dimensions, egg mass, and relative shell mass but did not affect size, mass, or total shell mass of the clutch...
May 14, 2018: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
https://www.readbyqxmd.com/read/29761068/antibiotic-containing-bone-substitute-in-major-hip-surgery-a-long-term-gentamicin-elution-study
#12
Mindaugas Stravinskas, Malin Nilsson, Peter Horstmann, Michael Mørk Petersen, Sarunas Tarasevicius, Lars Lidgren
Objectives: The objective is to present the antibiotic elution from a locally implanted gentamicin containing hydroxyapatite and calcium sulphate bone substitute with an extended follow up of 30 days. We also compare the pharmacokinetics of the ceramic bone substitute with a published study on gentamicin containing poly (methyl methacrylate) (PMMA) bone cement used in primary total hip arthroplasty. Methods: Gentamicin release was measured in the urine for a month and the serum for 4 days in 10 patients operated for trochanteric hip fractures and 10 patients in uncemented hip revisions...
2018: Journal of Bone and Joint Infection
https://www.readbyqxmd.com/read/29760599/vascular-erectile-dysfunction-and-subclinical-cardiovascular-disease
#13
Zain Gowani, S M Iftekhar Uddin, Mohammadhassan Mirbolouk, Dawar Ayyaz, Kevin L Billups, Martin Miner, David I Feldman, Michael J Blaha
Purpose of review: We review the recent literature on the hypothesized temporal relationship between subclinical cardiovascular disease (CVD), vascular erectile dysfunction (ED), and clinical CVD. In addition, we combine emerging research with expert consensus guidelines such as The Princeton Consensus III to provide a preventive cardiologist's perspective toward an ideal approach to evaluating and managing CVD and ED risk in patients. Recent findings: Development of ED was found to occur during the progression from subclinical CVD to clinical CVD...
December 2017: Current Sexual Health Reports
https://www.readbyqxmd.com/read/29757222/association-of-habitually-low-intake-of-dietary-calcium-with-blood-pressure-and-hypertension-in-a-population-with-predominantly-plant-based-diets
#14
Ziqi Liu, Aiping Fang, Jingjing He, Xin Shen, Rong Gao, Xintian Zhao, Keji Li
This study aimed to assess the association of habitually low dietary calcium intake with blood pressure or hypertensive risk using data from the China Health and Nutrition Survey (CHNS) in 2009. We included 6298 participants (2890 men and 3408 women) aged 18 years or older in this analysis. Food intakes were measured by 3-day 24-h individual recalls combined with a weighing and measuring of household food inventory. The participants were divided into normotensive, pre-hypertensive and hypertensive groups according to their mean blood pressure of three repeated measurements...
May 12, 2018: Nutrients
https://www.readbyqxmd.com/read/29756708/hypercalcemia-and-hypophosphatemia-among-preterm-infants-receiving-aggressive-parenteral-nutrition
#15
Pablo H Brener Dik, María F Galletti, Leticia T Bacigalupo, Silvia Fernández Jonusas, Gonzalo L Mariani
INTRODUCTION: Aggressive parenteral nutrition is the standard of care among very-low-birth weight preterm infants. However, in recent studies, its impact on short-term outcomes, has been evaluated. The objective was to compare the prevalence of hypercalcemia and hypophosphatemia among preterm infants receiving aggressive or standard parenteral nutrition. METHODS: Observational, retrospective study comparing a group of preterm infants weighing less than 1250 grams who received aggressive parenteral nutrition with a historical control group...
June 1, 2018: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/29756156/calcite-crystallization-in-the-cement-system-morphological-diversity-growth-mechanism-and-shape-evolution
#16
Jinyang Jiang, Qi Zheng, Dongshuai Hou, Yiru Yan, Heng Chen, Wei She, Shengping Wu, Dong Guo, Wei Sun
Carbonation plays an indispensable role in engineering construction, embracing mineralization, CO2 sequestration and low pH induced corrosion, but the essential mechanism of carbonation occurring in calcium silicate hydrate or portlandite can hardly be interpreted. Observation on how carbonation proceeds at the nano scale is thus critical for a better understanding of its dynamics. Here, using electron microscopy combined with first-principles calculation, a new view on carbonation in the cement system is revealed, considering morphological diversity, growth mechanism and shape evolution...
May 14, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29753733/coronary-computed-tomographic-angiography-findings-and-their-therapeutic-implications-in-asymptomatic-patients-with-familial-hypercholesterolemia-lessons-from-the-safeheart-study
#17
Leopoldo Pérez de Isla, Rodrigo Alonso, Ovidio Muñiz-Grijalvo, José Luis Díaz-Díaz, Daniel Zambón, José Pablo Miramontes, Francisco Fuentes, José Juan Gómez de Diego, Aurora González-Estrada, Nelva Mata, Adriana Saltijeral, Manuel Barreiro, Marta Tomás, Raimundo de Andrés, Rosa Argüeso, Maria Pilar Serrano Gotarredona, Silvia Navarro Herrero, Rosario J Perea Palazón, Teresa M de Caralt, Luisa Arrojo Suárez de Centi, Svetlana Zhilina, Simona Espejo Pérez, Teresa Padró, Pedro Mata
BACKGROUND: Familial hypercholesterolemia (FH) confers an increased risk of premature atherosclerotic disease. Coronary computed tomographic angiography (CTA) can assess preclinical coronary atherosclerosis. OBJECTIVES: To describe coronary CTA findings in asymptomatic molecularly defined FH individuals, to identify those factors related to its presence and extension, and to assess the impact of these results in patients' care and estimated risk. METHODS: Four hundred and forty individuals with FH, without clinical cardiovascular disease, were consecutively enrolled and underwent a coronary CTA that was used to analyze coronary atherosclerosis based on coronary calcium score (CCS), sum of stenosis severity, and plaque composition sum (PCS)...
April 17, 2018: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/29752690/spatio-temporal-dynamics-of-calcium-electrotransfer-during-cell-membrane-permeabilization
#18
Alexis Guionet, S Moosavi Nejad, Justin Teissié, Takashi Sakugawa, Sunao Katsuki, Hidenori Akiyama, Hamid Hosseini
Pulsed electric fields (PEFs) are applied as physical stimuli for DNA/drug delivery, cancer therapy, gene transformation, and microorganism eradication. Meanwhile, calcium electrotransfer offers an interesting approach to treat cancer, as it induces cell death easier in malignant cells than in normal cells. Here, we study the spatial and temporal cellular responses to 10 μs duration PEFs; by observing real-time, the uptake of extracellular calcium through the cell membrane. The experimental setup consisted of an inverted fluorescence microscope equipped with a color high-speed framing camera and a specifically designed miniaturized pulsed power system...
May 11, 2018: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29751309/modeling-salinization-and-recovery-of-road-salt-impacted-lakes-in-temperate-regions-based-on-long-term-monitoring-of-lake-george-new-york-usa-and-its-drainage-basin
#19
J W Sutherland, S A Norton, J W Short, C Navitsky
Road salt mitigates winter highway icing but accumulates in watershed soils and receiving waters, affecting soil chemistry and physical, biological, and ecological processes. Despite efforts to reduce salt loading in watersheds, accumulated cations and Cl- continue to impact tributaries and lakes, and the recovery process is not well understood. Lake George, New York (USA) is typical of many temperate lakes at risk for elevated Cl- concentrations from winter deicing; the lake salt concentration increased by ~3...
May 8, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29751286/the-prevalence-and-correlates-of-subclinical-atherosclerosis-among-adults-with-low-density-lipoprotein-cholesterol-70-mg-dl-the-multi-ethnic-study-of-atherosclerosis-mesa-and-brazilian-longitudinal-study-of-adult-health-elsa-brasil
#20
Mahmoud Al Rifai, Seth S Martin, John W McEvoy, Khurram Nasir, Ron Blankstein, Joseph Yeboah, Michael Miedema, Steven J Shea, Joseph F Polak, Pamela Ouyang, Roger S Blumenthal, Marcio Bittencourt, Isabela Bensenor, Raul D Santos, Bruce B Duncan, Itamar S Santos, Paulo A Lotufo, Michael J Blaha
BACKGROUND AND AIMS: The prevalence and correlates of subclinical atherosclerosis when low-density lipoprotein cholesterol (LDL-C) levels are low remain unclear. Therefore, we examined the association of cardiovascular risk factors and subclinical atherosclerosis among individuals with untreated LDL-C <70 mg/dL. METHODS: We included participants from the Multi-Ethnic Study of Atherosclerosis (MESA) and the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) cohorts...
April 28, 2018: Atherosclerosis
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