Yu Zhang, Jun Zhou, Ling Hua, Pan Li, Jiaqi Wu, Shenglan Shang, Fei Deng, Jing Luo, Mengling Liao, Nuoyan Wang, Xichun Pan, Yue Yuan, Yue Zheng, Yonglin Lu, Yasi Huang, Jiang Zheng, Xin Liu, Xiaoli Li, Hong Zhou
It is unclear whether membrane vitamin D receptor (mVDR) exists on the macrophage membrane or whether mVDR is associated with lipopolysaccharide (LPS) tolerance. Herein, we report that interfering with caveolae and caveolae-dependent lipid rafts inhibited the formation of LPS tolerance. VDR was detected as co-localized with membrane molecular markers. VDR was detected on the cell membrane and its level was higher in LPS-tolerant cells than that in only LPS treatment cells. Anti-VDR antibodies could abolish the effect of artesunate (AS) to reverse LPS tolerance, and the wild-type peptides (H397 and H305) of VDR, but not the mutant peptide (H397D and H305A), led to the loss of AS's effect...
May 29, 2023: Cell Communication and Signaling: CCS