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Herpes simplex 1

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https://www.readbyqxmd.com/read/28734843/a-high-resolution-melting-hrm-technology-based-assay-for-cost-efficient-clinical-detection-and-genotyping-of-herpes-simplex-virus-hsv-1-and-hsv-2
#1
M Lieveld, A Carregosa, I Benoy, N Redzic, M Berth, D Vanden Broeck
Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2...
July 19, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28732069/saccharomyces-boulardii-cncm-i-745-supplementation-reduces-gastrointestinal-dysfunction-in-an-animal-model-of-ibs
#2
Paola Brun, Melania Scarpa, Chiara Marchiori, Gloria Sarasin, Valentina Caputi, Andrea Porzionato, Maria Cecilia Giron, Giorgio Palù, Ignazio Castagliuolo
BACKGROUND: We evaluated the effect of Saccharomyces boulardii CNCM I-745 on intestinal neuromuscular anomalies in an IBS-type mouse model of gastrointestinal motor dysfunctions elicited by Herpes Simplex Virus type 1 (HSV-1) exposure. METHODS: Mice were inoculated intranasally with HSV-1 (102 PFU) or vehicle at time 0 and 4 weeks later by the intragastric (IG) route (108 PFU). Six weeks after IG inoculum, mice were randomly allocated to receive oral gavage with either S...
2017: PloS One
https://www.readbyqxmd.com/read/28729548/multiplex-polymerase-chain-reaction-for-pathogen-detection-in-donor-recipient-corneal-transplant-tissue-and-donor-storage-solution
#3
Takehiro Hariya, Kazuichi Maruyama, Sunao Sugita, Masayo Takahashi, Shunji Yokokura, Kota Sato, Yasuhiro Tomaru, Norio Shimizu, Toru Nakazawa
Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this study investigated a new technique, based on multiplex polymerase chain reaction (mPCR), to identify pathogens, including viruses, bacteria, and fungi, in corneal transplantation recipient sites, donor corneas and the donor cornea storage solution...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28723708/subclinical-herpesvirus-shedding-among-hiv-1-infected-men-on-antiretroviral-therapy
#4
Arcadio Agudelo-Hernandez, Yue Chen, Arlene Bullotta, William G Buchanan, Cynthia R Klamar-Blain, Luann Borowski, Sharon A Riddler, Charles R Rinaldo, Bernard Jc Macatangay
DESIGN: We evaluated the subclinical shedding of 6 different herpesviruses in antiretroviral drug-treated HIV(+) men who have sex with men (MSM), and determined how this is associated with markers of inflammation and immune activation. METHODS: We obtained blood, semen, throat washing, urine, and stool from 15 antiretroviral-treated HIV-1-infected MSM with CD4 T cell reconstitution, and 12 age-matched HIV(-) MSM from the Multicenter AIDS Cohort Study at 4 timepoints over 24 weeks to measure DNA levels of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus 1 and 2, human herpesvirus 6 (HHV6), and HHV8...
July 18, 2017: AIDS
https://www.readbyqxmd.com/read/28723706/oral-shedding-of-herpesviruses-in-hiv-patients-with-varying-degrees-of-immune-status
#5
Dirk P Dittmer, Kristen Tamburro, Huichao Chen, Anthony Lee, Marcia K Sanders, Tischan A Wade, Sonia Napravnik, Jennifer Webster-Cyriaque, Mahmoud Ghannoum, Caroline H Shiboski, Judith A Aberg
OBJECTIVE: Herpesvirus shedding in the oral cavity was analyzed to determine if presence in the oral compartment correlates with systemic changes in HIV-associated immune deficiency as measured by CD4+ counts, plasma HIV viral load (VL) and presence of AIDS-defining events. DESIGN: A5254 is a multicenter, cross-sectional, single-visit study to evaluate oral complications of HIV/AIDS and determine the association between clinical appearance, herpesvirus shedding and immune status as ascertained by CD4 count and HIV viral load...
July 18, 2017: AIDS
https://www.readbyqxmd.com/read/28722502/chemical-analysis-and-antiviral-activity-evaluation-of-baccharis-anomala
#6
Caroline Rita Venturi, Sérgio Antônio Loreto Bordignon, Paulo Michel Roehe, Jarbas Alves Montanha, Samuel Paulo Cibulski, Grace Gosmann
The chemical composition and antiviral activity of aqueous extract from Baccharis anomala was studied by bioactivity-guided fractionation. Ethanol precipitation and fractionation by molecular permeation allowed the separation of the anti-herpes simplex virus 1 (HSV-1) active fraction from aqueous extract (Fraction B). Natural Product Reagent A, FeCl3 and thin-layer chromatography indicated the presence of phenolic compounds in the aqueous extract. Fraction B showed pronounced antiviral activity when tested with HSV-1 strains VR733/ATCC and Acyclovir-resistant 29-R, displaying virucidal but not virustatic activity...
July 19, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28722019/sustained-accumulation-of-antigen-presenting-cells-after-infection-promotes-local-t-cell-immunity
#7
Nicholas Collins, Katharina Hochheiser, Francis R Carbone, Thomas Gebhardt
Antigen-presenting cells (APC) such as dendritic cells (DC) and macrophages are critical for T cell-mediated immunity. While it is established that memory T cells accumulate and persist in peripheral tissues after the resolution of infection, whether this is also the case for APC remains unclear. Here, we report that CCR2-dependent cells infiltrate skin during acute infection with herpes simplex virus (HSV)-1 and subsequently give rise to localized populations of dendritic cells and macrophages. These APC are found at elevated numbers at sites of resolved infection or inflammation compared to unaffected regions of skin...
July 19, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28717130/polymicrobial-infections-in-brain-tissue-from-alzheimer-s-disease-patients
#8
Diana Pisa, Ruth Alonso, Ana M Fernández-Fernández, Alberto Rábano, Luis Carrasco
Several studies have advanced the idea that the etiology of Alzheimer's disease (AD) could be microbial in origin. In the present study, we tested the possibility that polymicrobial infections exist in tissue from the entorhinal cortex/hippocampus region of patients with AD using immunohistochemistry (confocal laser scanning microscopy) and highly sensitive (nested) PCR. We found no evidence for expression of early (ICP0) or late (ICP5) proteins of herpes simplex virus type 1 (HSV-1) in brain sections. A polyclonal antibody against Borrelia detected structures that appeared not related to spirochetes, but rather to fungi...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716140/modelling-infectious-diseases-with-relapse-a-case-study-of-hsv-2
#9
Jinliang Wang, Xiaoqing Yu, Heidi L Tessmer, Toshikazu Kuniya, Ryosuke Omori
BACKGROUND: Herpes Simplex Virus Type 2 (HSV-2) is one of the most common sexually transmitted diseases. Although there is still no licensed vaccine for HSV-2, a theoretical investigation of the potential effects of a vaccine is considered important and has recently been conducted by several researchers. Although compartmental mathematical models were considered for each special case in the previous studies, as yet there are few global stability results. RESULTS: In this paper, we formulate a multi-group SVIRI epidemic model for HSV-2, which enables us to consider the effects of vaccination, of waning vaccine immunity, and of infection relapse...
July 17, 2017: Theoretical Biology & Medical Modelling
https://www.readbyqxmd.com/read/28710842/severe-sequelae-of-erythema-multiforme-three-cases
#10
A Viarnaud, S Ingen-Housz-Oro, M Marque, L Valeyrie-Allanore, N Ortonne, J Gueudry, S Grootenboer-Mignot, M Muraine, E Bequignon, C Gagnière, F Schlemmer, P Wolkenstein, O Chosidow
Erythema multiforme (EM) is a skin condition for which main causes are infectious factors (Herpes simplex virus, Mycoplasma pneumoniae). Anti-plakin auto-antibodies are found in some patients (1) . EM must be distinguished from Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN), which are drug-induced in most cases (2-5) . This article is protected by copyright. All rights reserved.
July 14, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28710778/a-detection-method-for-the-rash-and-fever-illness-associated-viruses-using-multiplex-rt-pcr
#11
Yuki Matsushima, Tomomi Shimizu, Ikuko Doi, Fuminori Mizukoshi, Koo Nagasawa, Akihide Ryo, Hideaki Shimizu, Masae Kobayashi, Keiji Funatogawa, Noriko Nagata, Mariko Ishikawa, Ayako Komane, Nobuhiko Okabe, Yoshio Mori, Makoto Takeda, Hirokazu Kimura
To detect various viral genes in the patients with rash and fever illness (RFI), a new multiplex (reverse transcription) polymerase chain reaction [(RT)-PCR] method was constructed. New primer sets were designed for the detection of herpes simplex viruses 1 and 2 (HSV1 and 2), and Epstein-Barr virus (EBV). The newly designed and previously reported primer sets were used to detect 13 types of RFI-associated viruses by multiplex (RT)-PCR assay systems. Moreover, to eliminate non-specific PCR products, a double-stranded specific DNase was used for the digestion of double-stranded DNA derived from the templates in clinical specimens...
July 15, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28705656/impact-of-enteroviral-polymerase-chain-reaction-testing-on-length-of-stay-for-infants-60-days-old-or-younger
#12
Paul L Aronson, Todd W Lyons, Andrea T Cruz, Stephen B Freedman, Pamela J Okada, Alesia H Fleming, Joseph L Arms, Amy D Thompson, Suzanne M Schmidt, Jeffrey Louie, Michael J Alfonzo, Michael C Monuteaux, Lise E Nigrovic
OBJECTIVE: To determine the impact of a cerebrospinal fluid enterovirus polymerase chain reaction (PCR) test performance on hospital length of stay (LOS) in a large multicenter cohort of infants undergoing evaluation for central nervous system infection. STUDY DESIGN: We performed a planned secondary analysis of a retrospective cohort of hospitalized infants ≤60 days of age who had a cerebrospinal fluid culture obtained at 1 of 18 participating centers (2005-2013)...
July 10, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28705429/disinfection-of-tonometers-a-report-by-the-american-academy-of-ophthalmology
#13
Anna K Junk, Philip P Chen, Shan C Lin, Kouros Nouri-Mahdavi, Sunita Radhakrishnan, Kuldev Singh, Teresa C Chen
OBJECTIVE: To examine the efficacy of various disinfection methods for reusable tonometer prisms in eye care and to highlight how disinfectants can damage tonometer tips and cause subsequent patient harm. METHODS: Literature searches were conducted last in October 2016 in the PubMed and the Cochrane Library databases for original research investigations. Reviews, non-English language articles, nonophthalmology articles, surveys, and case reports were excluded. RESULTS: The searches initially yielded 64 unique citations...
July 11, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28704950/structure-activity-relationships-of-acyclic-selenopurine-nucleosides-as-antiviral-agents
#14
Pramod K Sahu, Tamima Umme, Jinha Yu, Gyudong Kim, Shuhao Qu, Siddhi D Naik, Lak Shin Jeong
A series of acyclic selenopurine nucleosides 3a-f and 4a-g were synthesized based on the bioisosteric rationale between oxygen and selenium, and then evaluated for antiviral activity. Among the compounds tested, seleno-acyclovir (4a) exhibited the most potent anti-herpes simplex virus (HSV)-1 (EC50 = 1.47 µM) and HSV-2 (EC50 = 6.34 µM) activities without cytotoxicity up to 100 µM, while 2,6-diaminopurine derivatives 4e-g exhibited significant anti-human cytomegalovirus (HCMV) activity, which is slightly more potent than the guanine derivative 4d, indicating that they might act as prodrugs of seleno-ganciclovir (4d)...
July 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28702476/regulatable-transgene-expression-for-prevention-of-chemotherapy-induced-peripheral-neuropathy
#15
Daisuke Kawata, Zetang Wu
Chemotherapy-induced peripheral neuropathy (CIPN) is a debilitating complication associated with drug treatment of cancer for which there are no effective strategies of prevention or treatment. In this study, we examined the effect of intermittent expression of neurotophin-3 (NT-3) or interleukin-10 (IL-10) from replication-defective herpes simplex virus (HSV)-based regulatable vectors delivered by subcutaneous inoculation to the dorsal root ganglion (DRG) on the development of paclitaxel-induced peripheral neuropathy...
September 15, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28702475/deletion-of-the-virion-host-shut-off-gene-enhances-neuronal-selective-transgene-expression-from-an-hsv-vector-lacking-functional-ie-genes
#16
Yoshitaka Miyagawa, Gianluca Verlengia, Bonnie Reinhart, Fang Han, Hiroaki Uchida, Silvia Zucchini, William F Goins, Michele Simonato, Justus B Cohen, Joseph C Glorioso
The ability of herpes simplex virus (HSV) to establish lifelong latency in neurons suggests that HSV-derived vectors hold promise for gene delivery to the nervous system. However, vector toxicity and transgene silencing have created significant barriers to vector applications to the brain. Recently, we described a vector defective for all immediate-early gene expression and deleted for the joint region between the two unique genome segments that proved capable of extended transgene expression in non-neuronal cells...
September 15, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28701403/monoclonal-antibodies-derived-from-humans-vaccinated-with-the-rv144-hiv-vaccine-containing-the-hvem-binding-domain-of-herpes-simplex-virus-hsv-glycoprotein-d-neutralize-hsv-infection-mediate-adcc-and-protect-mice-from-ocular-challenge-with-hsv-1
#17
Kening Wang, Georgia D Tomaras, Sinthujan Jegaskanda, M Anthony Moody, Hua-Xin Liao, Kyle Goodman, Phillip W Berman, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayapan, Jaranit Kaewkungwal, Barton F Haynes, Jeffrey I Cohen
The RV144 HIV vaccine trial included a recombinant HIV glycoprotein 120 (gp120) construct fused to a small portion of herpes simplex virus (HSV-1) glycoprotein D (gD), such that the first 40 amino acids of gp120 were replaced by the signal sequence and the first 27 amino acids of the mature form of gD. This region of gD contains most of the binding site for HVEM, an HSV receptor important for virus infection of epithelial cells and lymphocytes. RV144 induced antibodies to HIV that were partially protective against infection as well as antibodies to HSV...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28701399/selective-expression-of-ccr10-and-cxcr3-by-circulating-human-hsv-specific-cd8-t-cells
#18
Michael T Hensel, Tao Peng, Anqi Cheng, Stephen C De Rosa, Anna Wald, Kerry J Laing, Lichen Jing, Lichun Dong, Amalia S Magaret, David M Koelle
Herpes simplex virus type (HSV) infection is restricted to epithelial cells and neurons and is controlled by CD8 T-cells. These cells both traffic to epithelial sites of recurrent lytic infection and to ganglia, and persist at the dermal-epidermal junction for up to 12 weeks after lesion resolution. We previously showed that cutaneous lymphocyte-associated antigen (CLA), a functional E-selectin ligand (ESL), is selectively expressed on circulating HSV-2-specific CD8 T-cells. CLA/ESL mediates adhesion of T-cells to inflamed vascular endothelium...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28700944/viral-activation-of-heparanase-drives-pathogenesis-of-herpes-simplex-virus-1
#19
Alex M Agelidis, Satvik R Hadigal, Dinesh Jaishankar, Deepak Shukla
Herpes simplex virus-1 (HSV-1) causes lifelong recurrent pathologies without a cure. How infection by HSV-1 triggers disease processes, especially in the immune-privileged avascular human cornea, remains a major unresolved puzzle. It has been speculated that a cornea-resident molecule must tip the balance in favor of pro-inflammatory and pro-angiogenic conditions observed with herpetic, as well as non-herpetic, ailments of the cornea. Here, we demonstrate that heparanase (HPSE), a host enzyme, is the molecular trigger for multiple pathologies associated with HSV-1 infection...
July 11, 2017: Cell Reports
https://www.readbyqxmd.com/read/28699403/rna-seq-investigations-of-human-post-mortem-trigeminal-ganglia
#20
Danielle M LaPaglia, Matthew R Sapio, Peter D Burbelo, Jean Thierry-Mieg, Danielle Thierry-Mieg, Stephen J Raithel, Christopher E Ramsden, Michael J Iadarola, Andrew J Mannes
Background The trigeminal ganglion contains neurons that relay sensations of pain, touch, pressure, and many other somatosensory modalities to the central nervous system. The ganglion is also a reservoir for latent herpes virus 1 infection. To gain a better understanding of molecular factors contributing to migraine and headache, transcriptome analyses were performed on postmortem human trigeminal ganglia. Methods RNA-Seq measurements of gene expression were conducted on small sub-regions of 16 human trigeminal ganglia...
January 1, 2017: Cephalalgia: An International Journal of Headache
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