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https://www.readbyqxmd.com/read/28822048/evaluation-of-methods-to-determine-adsorption-of-polycyclic-aromatic-hydrocarbons-to-dispersed-carbon-nanotubes
#1
Berit Glomstad, Lisbet Sørensen, Jingfu Liu, Mohai Shen, Florian Zindler, Bjørn M Jenssen, Andy M Booth
A number of methods have been reported for determining hydrophobic organic compound adsorption to dispersed carbon nanotubes (CNTs), but their accuracy and reliability remain uncertain. We have evaluated three methods to investigate the adsorption of phenanthrene (a model polycyclic aromatic hydrocarbon, PAH) to CNTs with different physicochemical properties: dialysis tube (DT) protected negligible depletion solid phase microextraction (DT-nd-SPME), ultracentrifugation, and filtration using various types of filters...
August 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28821668/sensory-biased-and-multiple-demand-processing-in-human-lateral-frontal-cortex
#2
Abigail L Noyce, Nishmar Cestero, Samantha W Michalka, Barbara G Shinn-Cunningham, David C Somers
The functionality of much of human lateral frontal cortex (LFC) has been characterized as 'multiple demand' as these regions appear to support a broad range of cognitive tasks. In contrast to this domain-general account, recent evidence indicates that portions of LFC are consistently selective for sensory modality. Michalka et al. (2015) reported two bilateral regions that are biased for visual attention, superior precentral sulcus (sPCS) and inferior precentral sulcus (iPCS), interleaved with two bilateral regions that are biased for auditory attention, transverse gyrus intersecting precentral sulcus (tgPCS) and caudal inferior frontal sulcus (cIFS)...
August 8, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28821492/use-of-the-low-frequency-high-frequency-ratio-of-heart-rate-variability-to-predict-short-term-deterioration-in-emergency-department-patients-with-sepsis
#3
Douglas P Barnaby, Shannon M Fernando, Kevin J Ferrick, Christophe L Herry, Andrew J E Seely, Polly E Bijur, E John Gallagher
OBJECTIVE: To examine the ability of the low-frequency/high-frequency (LF/HF) ratio of heart rate variability (HRV) analysis to identify patients with sepsis at risk of early deterioration. METHODS: This is a prospective observational cohort study of patients with sepsis presenting to the Montefiore Medical Center ED from December 2014 through September 2015. On presentation, a single ECG Holter recording was obtained and analysed to obtain the LF/HF ratio of HRV...
August 18, 2017: Emergency Medicine Journal: EMJ
https://www.readbyqxmd.com/read/28820349/evaluation-of-different-methods-for-dna-extraction-from-human-burnt-bones-and-the-generation-of-genetic-profiles-for-identification
#4
Anum Uzair, Nouman Rasool, Muhammad Wasim
Bone exposure to heat in the presence of moisture breaks the phosphodiester bonds of the backbone, leaving sheared DNA in bone cells. This also limits the possibility of generating a complete profile of the victim. With the increasing incidence of fire outbreaks over the past few years, a paradigm shift to establish identity has been observed, from morphological identification of victims to STR profiling. For this study, 10 bone samples were taken from burnt human bodies that were recovered from different fire outbreak scenes...
January 1, 2017: Medicine, Science, and the Law
https://www.readbyqxmd.com/read/28817693/clinical-signs-profound-acidemia-hypoglycemia-and-hypernatremia-are-predictive-of-mortality-in-1-400-critically-ill-neonatal-calves-with-diarrhea
#5
Florian M Trefz, Ingrid Lorenz, Annette Lorch, Peter D Constable
Profound acidemia impairs cellular and organ function and consequently should be associated with an increased risk of mortality in critically ill humans and animals. Neonatal diarrhea in calves can result in potentially serious metabolic derangements including profound acidemia due to strong ion (metabolic) acidosis, hyper-D-lactatemia, hyper-L-lactatemia, azotemia, hypoglycemia, hyperkalemia and hyponatremia. The aim of this retrospective study was to assess the prognostic relevance of clinical and laboratory findings in 1,400 critically ill neonatal calves with diarrhea admitted to a veterinary teaching hospital...
2017: PloS One
https://www.readbyqxmd.com/read/28814576/children-s-hospitals-solutions-for-patient-safety-collaborative-impact-on-hospital-acquired-harm
#6
Anne Lyren, Richard J Brilli, Karen Zieker, Miguel Marino, Stephen Muething, Paul J Sharek
OBJECTIVES: To determine if an improvement collaborative of 33 children's hospitals focused on reliable best practice implementation and culture of safety improvements can reduce hospital-acquired conditions (HACs) and serious safety events (SSEs). METHODS: A 3-year prospective cohort study design with a 12-month historical control population was completed by the Children's Hospitals' Solutions for Patient Safety collaborative. Identification and dissemination of best practices related to 9 HACs and SSE reduction focused on key process and culture of safety improvements...
August 16, 2017: Pediatrics
https://www.readbyqxmd.com/read/28814514/ba71%C3%AE-cd2-a-new-recombinant-live-attenuated-african-swine-fever-virus-with-cross-protective-capabilities
#7
Paula L Monteagudo, Anna Lacasta, Elisabeth López, Laia Bosch, Javier Collado, Sonia Pina-Pedrero, Florencia Correa-Fiz, Francesc Accensi, María Jesús Navas, Enric Vidal, María J Bustos, Javier M Rodríguez, Andreas Gallei, Veljko Nikolin, María L Salas, Fernando Rodríguez
African swine fever is a highly contagious viral disease of mandatory declaration to the World Organization for Animal Health (OIE). Lack of available vaccines make its control difficult and thus ASFV represents a major threat to the swine industry. Inactivated vaccines do not confer solid protection against African swine fever virus (ASFV). Conversely, live attenuated viruses (LAV), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable protection against homologous ASFV strains, albeit little or no protection has been demonstrated against heterologous viruses...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28812572/population-characteristics-of-a-large-whale-shark-aggregation-inferred-from-seawater-environmental-dna
#8
Eva Egelyng Sigsgaard, Ida Broman Nielsen, Steffen Sanvig Bach, Eline D Lorenzen, David Philip Robinson, Steen Wilhelm Knudsen, Mikkel Winther Pedersen, Mohammed Al Jaidah, Ludovic Orlando, Eske Willerslev, Peter Rask Møller, Philip Francis Thomsen
Population genetics is essential for understanding and managing marine ecosystems, but sampling remains challenging. We demonstrate that high-throughput sequencing of seawater environmental DNA can provide useful estimates of genetic diversity in a whale shark (Rhincodon typus) aggregation. We recover similar mitochondrial haplotype frequencies in seawater compared to tissue samples, reliably placing the studied aggregation in a global genetic context and expanding the applications of environmental DNA to encompass population genetics of aquatic organisms...
November 21, 2016: Nature ecology & evolution
https://www.readbyqxmd.com/read/28812085/high-tc-superconducting-phases-in-organic-molecular-intercalated-iron-selenides-synthesis-and-crystal-structures
#9
Shifeng Jin, Xiao Fan, Xiaozhi Wu, Ruijin Sun, Hui Wu, Qingzhen Huang, Chenlong Shi, Xuekui Xi, Zhilin Li, Xiaolong Chen
Hybrid iron-based superconductors were synthesized by sonochemical insertion of organic molecules into FeSe layers. High quality of the samples first enabled reliable phase identifications and three structure types were discovered. Structure determination based on neutron data further facilitated the understanding of their structural stability, doping levels and temperature driven structural transitions.
August 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28809829/horizontal-whole-mount-a-novel-processing-and-imaging-protocol-for-thick-three-dimensional-tissue-cross-sections-of-skin
#10
Lucia Salz, Ryan R Driskell
Processing a tissue of interest to generate a microscopic image that supports a scientific argument can be challenging. The acquisition of high-quality microscopic images is not entirely dependent upon the quality of the microscope, but also upon the methods of tissue processing, which often involve multiple critical actions or steps. Furthermore, mesenchymal cell types in the skin and other tissues represent a new challenge for tissue preparation and imaging. Here, we present a complete process, from tissue harvest to microscopy...
August 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28808091/inter-rater-reliability-and-downstream-financial-implications-of-electrocardiography-screening-in-young-athletes
#11
Harshil Dhutia, Aneil Malhotra, Tee Joo Yeo, Irina Chis Ster, Vincent Gabus, Alexandros Steriotis, Helder Dores, Greg Mellor, Carmen García-Corrales, Bode Ensam, Viknesh Jayalapan, Vivienne Anne Ezzat, Gherardo Finocchiaro, Sabiha Gati, Michael Papadakis, Maria Tome-Esteban, Sanjay Sharma
BACKGROUND: Preparticipation screening for cardiovascular disease in young athletes with electrocardiography is endorsed by the European Society of Cardiology and several major sporting organizations. One of the concerns of the ECG as a screening test in young athletes relates to the potential for variation in interpretation. We investigated the degree of variation in ECG interpretation in athletes and its financial impact among cardiologists of differing experience. METHODS AND RESULTS: Eight cardiologists (4 with experience in screening athletes) each reported 400 ECGs of consecutively screened young athletes according to the 2010 European Society of Cardiology recommendations, Seattle criteria, and refined criteria...
August 2017: Circulation. Cardiovascular Quality and Outcomes
https://www.readbyqxmd.com/read/28807812/evaluation-and-clinical-validation-of-two-field-deployable-reverse-transcription-insulated-isothermal-pcr-assays-for-the-detection-of-the-middle-east-respiratory-syndrome-coronavirus
#12
Yun Young Go, Yeon-Sook Kim, Shinhye Cheon, Sangwoo Nam, Keun Bon Ku, Meehyein Kim, Nam Hyuk Cho, Hyun Park, Pei-Yu Alison Lee, Yu-Chun Lin, Yun-Long Tsai, Hwa-Tang Thomas Wang, Udeni B R Balasuriya
Middle East respiratory syndrome (MERS) is an emerging zoonotic viral respiratory disease that was first identified in Saudi Arabia in 2012. In 2015, the largest MERS outbreak outside of the Middle East region occurred in the Republic of Korea. The rapid nosocomial transmission of MERS-coronavirus (MERS-CoV) in Korean healthcare settings highlighted the importance and urgent need for a rapid and reliable on-site diagnostic assay to implement effective control and preventive measures. Here, we describe the evaluation and validation of two newly developed reverse transcription-insulated isothermal PCR (RT-iiPCR) methods targeting the ORF1a and upE genes of MERS-CoV...
August 11, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28806912/genome-wide-comparative-analysis-of-dna-methylation-between-soybean-cytoplasmic-male-sterile-line-njcms5a-and-its-maintainer-njcms5b
#13
Yanwei Li, Xianlong Ding, Xuan Wang, Tingting He, Hao Zhang, Longshu Yang, Tanliu Wang, Linfeng Chen, Junyi Gai, Shouping Yang
BACKGROUND: DNA methylation is an important epigenetic modification. It can regulate the expression of many key genes without changing the primary structure of the genomic DNA, and plays a vital role in the growth and development of the organism. The genome-wide DNA methylation profile of the cytoplasmic male sterile (CMS) line in soybean has not been reported so far. RESULTS: In this study, genome-wide comparative analysis of DNA methylation between soybean CMS line NJCMS5A and its maintainer NJCMS5B was conducted by whole-genome bisulfite sequencing...
August 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28800693/a-modified-sintacs-method-for-groundwater-vulnerability-and-pollution-risk-assessment-in-highly-anthropized-regions-based-on-no3-and-so4-2-concentrations
#14
Busico Gianluigi, Kazakis Nerantzis, Colombani Nicolò, Mastrocicco Micòl, Voudouris Konstantinos, Tedesco Dario
Groundwater vulnerability and risk assessment area worldwide tools in supporting groundwater protection and land planning. In this study, we used three of these different methodologies applied to the Campanian Plain located in southern Italy: SINTACS, AVI and LOS. However, their capability to describe the observed chemical pollution of the area has resulted quite poor. For such a reason, a modified SINTACS method has been then implemented in the area in order to get a more reliable view of groundwater vulnerability...
August 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28797523/the-acute-toxic-effects-of-1-alkyl-3-methylimidazolium-nitrate-ionic-liquids-on-chlorella-vulgaris-and-daphnia-magna
#15
Cheng Zhang, Shuai Zhang, Lusheng Zhu, Jinhua Wang, Jun Wang, Tongtong Zhou
Given their increasingly widespread application, the toxic effects of ionic liquids (ILs) have become the subject of significant attention in recent years. Therefore, the present study assessed the acute toxic effects of 1-alkyl-3-methylimidazolium nitrate ([Cnmim]NO3 (n = 2, 4, 6, 8, 10, 12)) on Chlorella vulgaris and Daphnia magna. The sensitivity of the tested organism Daphnia magna and the investigated IL concentrations in water using high-performance liquid chromatography (HPLC) were also evaluated to demonstrate the reliability of the present study...
October 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28795166/an-efficient-strategy-using-k-mers-to-analyse-16s-rrna-sequences
#16
Marcel Martínez-Porchas, Francisco Vargas-Albores
The use of k-mers has been a successful strategy for improving metagenomics studies, including taxonomic classifications, or de novo assemblies, and can be used to obtain sequences of interest from the available databases. The aim of this manuscript was to propose a simple but efficient strategy to generate k-mers and to use them to obtain and analyse in silico 16S rRNA sequence fragments. A total of 513,309 bacterial sequences contained in the SILVA database were considered for the study, and homemade PHP scripts were used to search for specific nucleotide chains, recover fragments of bacterial sequences, make calculations and organize information...
July 2017: Heliyon
https://www.readbyqxmd.com/read/28792274/global-regional-and-national-prevalence-incidence-and-disability-adjusted-life-years-for-oral-conditions-for-195-countries-1990-2015-a-systematic-analysis-for-the-global-burden-of-diseases-injuries-and-risk-factors
#17
N J Kassebaum, A G C Smith, E Bernabé, T D Fleming, A E Reynolds, T Vos, C J L Murray, W Marcenes
The Global Burden of Disease 2015 study aims to use all available data of sufficient quality to generate reliable and valid prevalence, incidence, and disability-adjusted life year (DALY) estimates of oral conditions for the period of 1990 to 2015. Since death as a direct result of oral diseases is rare, DALY estimates were based on years lived with disability, which are estimated only on those persons with unmet need for dental care. We used our data to assess progress toward the Federation Dental International, World Health Organization, and International Association for Dental Research's oral health goals of reducing the level of oral diseases and minimizing their impact by 2020...
April 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28789788/alexithymia-and-anesthetic-bladder-capacity-in-interstitial-cystitis-bladder-pain-syndrome
#18
Chui-De Chiu, Ming-Huei Lee, Wei-Chih Chen, Hoi Lam Ho, Huei-Ching Wu
OBJECTIVE: In contrast to the inconsistent results of organic causes, it has been found that psychological risk factors are reliably related to functional somatic syndromes (FSSs), including interstitial cystitis/bladder pain syndrome (IC/BPS). Compared to patients with acute cystitis, a subgroup of IC/BPS patients with a history of childhood relational trauma reported intensified unregulated affective states (i.e., anxiety and depression) and trauma-related psychopathology (i.e., dissociation)...
September 2017: Journal of Psychosomatic Research
https://www.readbyqxmd.com/read/28783915/comparison-of-pocis-passive-samplers-vs-composite-water-sampling-a-case-study
#19
Justine Criquet, David Dumoulin, Michael Howsam, Leslie Mondamert, Jean-François Goossens, Jean Prygiel, Gabriel Billon
The relevance of Polar Organic Chemical Integrative Samplers (POCIS) was evaluated for the assessment of concentrations of 46 pesticides and 19 pharmaceuticals in a small, peri-urban river with multi-origin inputs. Throughout the period of POCIS deployment, 24h-average water samples were collected automatically, and showed the rapid temporal evolution of concentrations of several micropollutants, as well as permitting the calculation of average concentrations in the water phase for comparison with those estimated from POCIS passive samplers...
August 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28776982/flux-analysis-of-free-amino-sugars-and-amino-acids-in-soils-by-isotope-tracing-with-a-novel-liquid-chromatography-high-resolution-mass-spectrometry-platform
#20
Yuntao Hu, Qing Zheng, Wolfgang Wanek
Soil fluxomics analysis can provide pivotal information for understanding soil biochemical pathways and their regulation, but direct measurement methods are rare. Here, we describe an approach to measure soil extracellular metabolite (amino sugar and amino acid) concentrations and fluxes based on a (15)N isotope pool dilution technique via liquid chromatography and high-resolution mass spectrometry. We produced commercially unavailable (15)N and (13)C labeled amino sugars and amino acids by hydrolyzing peptidoglycan isolated from isotopically labeled bacterial biomass and used them as tracers ((15)N) and internal standards ((13)C)...
August 22, 2017: Analytical Chemistry
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