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High reliability organization

Younes Mohamed Ismail Hani, Cyril Turies, Olivier Palluel, Laurence Delahaut, Véronique Gaillet, Anne Bado-Nilles, Jean-Marc Porcher, Alain Geffard, Odile Dedourge-Geffard
The development of predictive, sensitive and reliable biomarkers is of crucial importance for aquatic biomonitoring to assess the effects of chemical substances on aquatic organisms, especially when it comes to combined effects with other stressors (e.g. temperature). The first purpose of the present study was to evaluate the single and combined effects of 90 days of exposure to an environmental cadmium concentration (0.5 μg L-1 ) and two water temperatures (16 and 21 °C) on different parameters. These parameters are involved in (i) the antioxidant system (superoxide dismutase activity -SOD- and total glutathione levels -GSH-), (ii) the energy metabolism, i...
April 12, 2018: Aquatic Toxicology
Andrew Hylton, Ylenia Chiari, Isabella Capellini, Mace G Barron, Scott Glaberman
Chemical use in society is growing rapidly and is one of the five major pressures on biodiversity worldwide. Since empirical toxicity studies of pollutants generally focus on a handful of model organisms, reliable approaches are needed to assess sensitivity to chemicals across the wide variety of species in the environment. Phylogenetic comparative methods (PCM) offer a promising approach for toxicity extrapolation incorporating known evolutionary relationships among species. If phylogenetic signal in toxicity data is high, i...
April 2018: Ecological Applications: a Publication of the Ecological Society of America
David Nasralla, Constantin C Coussios, Hynek Mergental, M Zeeshan Akhtar, Andrew J Butler, Carlo D L Ceresa, Virginia Chiocchia, Susan J Dutton, Juan Carlos García-Valdecasas, Nigel Heaton, Charles Imber, Wayel Jassem, Ina Jochmans, John Karani, Simon R Knight, Peri Kocabayoglu, Massimo Malagò, Darius Mirza, Peter J Morris, Arvind Pallan, Andreas Paul, Mihai Pavel, M Thamara P R Perera, Jacques Pirenne, Reena Ravikumar, Leslie Russell, Sara Upponi, Chris J E Watson, Annemarie Weissenbacher, Rutger J Ploeg, Peter J Friend
Liver transplantation is a highly successful treatment, but is severely limited by the shortage in donor organs. However, many potential donor organs cannot be used; this is because sub-optimal livers do not tolerate conventional cold storage and there is no reliable way to assess organ viability preoperatively. Normothermic machine perfusion maintains the liver in a physiological state, avoids cooling and allows recovery and functional testing. Here we show that, in a randomized trial with 220 liver transplantations, compared to conventional static cold storage, normothermic preservation is associated with a 50% lower level of graft injury, measured by hepatocellular enzyme release, despite a 50% lower rate of organ discard and a 54% longer mean preservation time...
April 18, 2018: Nature
Ross C Lagoy, Dirk R Albrecht
High-throughput biological and chemical experiments typically use either multiwell plates or microfluidic devices to analyze numerous independent samples in a compact format. Multiwell plates are convenient for screening chemical libraries in static fluid environments, whereas microfluidic devices offer immense flexibility in flow control and dynamics. Interfacing these platforms in a simple and automated way would introduce new high-throughput experimental capabilities, such as compound screens with precise exposure timing...
April 18, 2018: Scientific Reports
Hailong Wang, Quanqin Hu, Yuan Meng, Zier Jin, Zilin Fang, Qinrui Fu, Wenhua Gao, Liang Xu, Yibing Song, Fushen Lu
Reduced graphite oxide (rGO) was incorporated into a metal organic framework (MOF) MIL-101(Cr) for the modification of carbon paste electrode. Taking advantages of the large surface area of MOF and the electrical conductivity of rGO, the resulted electrodes exhibited high sensitivity and reliability in the simultaneous electrochemical identification and quantification of catechol (CC) and hydroquinone (HQ). Specifically, in the mixture solution of catechol and hydroquinone (constant concentration of an analyte), the linear response ranges for catechol and hydroquinone were 10-1400 μM and 4-1000 μM, and detection limits were 4 μM and 0...
February 19, 2018: Journal of Hazardous Materials
Alfonso Rodríguez-Gil, Tabea Riedlinger, Olesja Ritter, Vera V Saul, M Lienhard Schmitz
Appropriate gene expression in response to extracellular cues, that is, tissue- and lineage-specific gene transcription, critically depends on highly defined states of chromatin organization. The dynamic architecture of the nucleus is controlled by multiple mechanisms and shapes the transcriptional output programs. It is, therefore, important to determine locus-specific chromatin accessibility in a reliable fashion that is preferably independent from antibodies, which can be a potentially confounding source of experimental variability...
April 2, 2018: Journal of Visualized Experiments: JoVE
Xianquan Zhan, Yuda Huang, Ying Long
Human pituitary adenoma (PA) is a common tumor that occurs in the human pituitary gland in the hypothalamus-pituitary-targeted organ axis systems, and may be classified as either clinically functional or nonfunctional PA (FPA and NFPA). NFPA is difficult for early stage diagnosis and therapy due to barely elevating hormones in the blood compared to FPA. Our long-term goal is to use proteomics methods to discover reliable biomarkers for clarification of PA molecular mechanisms and recognition of effective diagnostic, prognostic markers and therapeutic targets...
April 2, 2018: Journal of Visualized Experiments: JoVE
Sophia Houari, Sylvie Babajko, Sophia Loiodice, Ariane Berdal, Katia Jedeon
Enamel defects resulting from environmental conditions and ways of life are public health concerns because of their high prevalence. These defects result from altered activity of cells responsible for enamel synthesis named ameloblasts, which present in enamel organ. During amelogenesis, ameloblasts follow a specific and precise sequence of events of proliferation, differentiation, and death. A rat continually growing incisors is a suitable experimental model to study ameloblast activity and differentiation stages in physiological and pathological conditions...
March 29, 2018: Journal of Visualized Experiments: JoVE
Marc T Edwards
In pursuit of high reliability, numerous organizations have promoted Just Culture, but its impact has never been assessed. This report combines data from a longitudinal survey-based study of clinical peer review practices in a cohort of 457 acute care hospitals with 43 measures from the Hospital Compare database and interprets them in relation to the long-term trends of Agency for Healthcare Research and Quality (AHRQ) data on the Hospital Survey of Patient Safety Culture. In all, 211 of 270 respondents (79%) indicated that their hospital has adopted Just Culture...
April 1, 2018: American Journal of Medical Quality: the Official Journal of the American College of Medical Quality
Rebecca L Berman, Madelyn Iris, Kendon J Conrad, Carrie Robinson
BACKGROUND: Older adults taking multiple prescription and nonprescription drugs are at risk for medication use problems, yet there are few brief, self-administered screening tools designed specifically for them. OBJECTIVE: The study objective was to develop and validate a patient-centered screener for community-dwelling older adults. METHODS: In phase 1, a convenience sample of 57 stakeholders (older adults, pharmacists, nurses, and physicians) participated in concept mapping, using Concept System® Global MAXTM , to identify items for a questionnaire...
January 1, 2018: Journal of Pharmacy Practice
Kolja N Eckermann, Hassan M M Ahmed, Mohammad KaramiNejadRanjbar, Stefan Dippel, Christian E Ogaugwu, Peter Kitzmann, Musa D Isah, Ernst A Wimmer
Even in times of advanced site-specific genome editing tools, the improvement of DNA transposases is still on high demand in the field of transgenesis: especially in emerging model systems where evaluated integrase landing sites have not yet been created and more importantly in non-model organisms such as agricultural pests and disease vectors, in which reliable sequence information and genome annotations are still pending. In fact, random insertional mutagenesis is essential to identify new genomic locations that are not influenced by position effects and thus can serve as future stable transgene integration sites...
April 10, 2018: Insect Biochemistry and Molecular Biology
Yadong Tang, Lu Xie, Lanming Chen
Apoptosis proteins (APs) control normal tissue homeostasis by regulating the balance between cell proliferation and death. The function of APs is strongly related to their subcellular location. To date, computational methods have been reported that reliably identify the subcellular location of APs, however, there is still room for improvement of the prediction accuracy. In this study, we developed a novel method named iAPSL-IF (identification of apoptosis protein subcellular location-integrative features), which is based on integrative features captured from Markov chains, physicochemical property matrices, and position-specific score matrices (PSSMs) of amino acid sequences...
April 13, 2018: International Journal of Molecular Sciences
Sophie I Mavrogeni, George Kitas, Hildo J Lamb, Klearchos Psychoyios, Theodoros Dimitroulas, Loukia Koutsogeorgopoulou, Kyriaki Boki, Vasiliki Vartela, Genovefa Kolovou, George Markousis-Mavrogenis, Cees G Kallenberg, Loic Guillevin, Dimitrios Vassilopoulos
Systemic vasculitides (SVs) is a group of diseases characterised by inflammation/necrosis of the blood vessel wall in various organs. Simultaneous brain and heart involvement is a cause of increased morbidity/mortality in SV. We aimed to present evidence of concurrent brain/heart involvement in SV and the role of a combined brain/heart magnetic resonance imaging (MRI) in their risk stratification. Cerebral vasculitis (CV) can be presented as focal deficits, seizures, headache, neuropsychiatric manifestations or cognitive dysfunction and cardiovascular disease (CVD) as myocardial/vascular inflammation, perfusion/function defects and fibrosis...
April 10, 2018: Clinical and Experimental Rheumatology
Michael A Vogelbaum, Cathy Brewer, Gene H Barnett, Alireza M Mohammadi, David M Peereboom, Manmeet S Ahluwalia, Shenqiang Gao
OBJECTIVE Progress in management of high-grade gliomas (HGGs) has been hampered by poor access of potential therapeutics to the CNS. The Cleveland Multiport Catheter (CMC), which deploys 4 independent delivery microcatheters, was developed to be a reliable, high-volume delivery device for delivery of therapeutic agents to the brain and other solid organs. The authors undertook this first-in-human clinical trial effort to evaluate the delivery characteristics of the CMC in patients with HGGs. METHODS A series of pilot studies were launched after approval of a sponsor-investigator IND (investigational new drug) application to evaluate the delivery of topotecan and gadolinium-DTPA (Gd-DTPA) via the CMC in patients with recurrent HGG...
April 13, 2018: Journal of Neurosurgery
Chenqi Niu, Yuancong Xu, Chao Zhang, Pengyu Zhu, Kunlun Huang, Yunbo Luo, Wentao Xu
As genetically modified (GM) technology develops and genetically modified organisms (GMOs) become more available, GMOs face increasing regulations and pressure to adhere to strict labeling guidelines. A singleplex detection method cannot perform the high-throughput analysis necessary for optimal GMO detection. Combining the advantages of multiplex detection and droplet digital polymerase chain reaction (ddPCR), a single universal primer-multiplex-ddPCR (SUP-M-ddPCR) strategy was proposed for accurate broad-spectrum screening and quantification...
April 13, 2018: Analytical Chemistry
Katarína Kimáková, Linda Petijová, Katarína Bruňáková, Eva Čellárová
Higher plants often accumulate secondary metabolites in multicellular structures or in secretory reservoirs. Biotechnological production of such compounds by cell cultures lacking proper morphological structures is difficult, therefore possibilities for an efficient increase of their formation by organ cultures are being searched. The genus Hypericum comprises many species that store photoactive and phototoxic naphthodianthrones in the dark nodules on their above-ground parts. To date, the relation between the content of hypericins and their proto-forms accumulated in the nodules, and morphological characters of the plant parts containing these structures has not been sufficiently explained...
June 2018: Plant Science: An International Journal of Experimental Plant Biology
Zhenyu Cui, Yanjun Gao, Wenzeng Yang, Chunli Zhao, Tao Ma, Xiaoqiang Shi
Objective: To evaluate the therapeutic effects of visual standard channel combined with F4.8 visual puncture super-mini percutaneous nephrolithotomy (SMP) on multiple renal calculi. Methods: The clinical data of 46 patients with multiple renal calculi treated in Affiliated Hospital of Hebei University from October 2015 to September 2016 were retrospectively analyzed. There were 28 males and 18 females aged from 25 to 65 years old, with an average of 42.6. The stone diameters were 3...
January 2018: Pakistan Journal of Medical Sciences Quarterly
Pathima Nusrath Hameed, Karin Verspoor, Snezana Kusljic, Saman Halgamuge
BACKGROUND: Drug repositioning is the process of identifying new uses for existing drugs. Computational drug repositioning methods can reduce the time, costs and risks of drug development by automating the analysis of the relationships in pharmacology networks. Pharmacology networks are large and heterogeneous. Clustering drugs into small groups can simplify large pharmacology networks, these subgroups can also be used as a starting point for repositioning drugs. In this paper, we propose a two-tiered drug-centric unsupervised clustering approach for drug repositioning, integrating heterogeneous drug data profiles: drug-chemical, drug-disease, drug-gene, drug-protein and drug-side effect relationships...
April 11, 2018: BMC Bioinformatics
Jonathan Mercier, Adrien Josso, Claudine Médigue, David Vallenet
BACKGROUND: High quality functional annotation is essential for understanding the phenotypic consequences encoded in a genome. Despite improvements in bioinformatics methods, millions of sequences in databanks are not assigned reliable functions. The curation of protein functions in the context of biological processes is a way to evaluate and improve their annotation. RESULTS: We developed an expert system using paraconsistent logic, named GROOLS (Genomic Rule Object-Oriented Logic System), that evaluates the completeness and the consistency of predicted functions through biological processes like metabolic pathways...
April 11, 2018: BMC Bioinformatics
Süheyla Sürücüoğlu
Tuberculosis has spread by human movements throughout history. There have been reports indicating tuberculosis transmission on all travel vehicles, including aircrafts, ground vehicles and vessels until today. However, due to the ever increasing of air transportation and air travelling among countries with low and high tuberculosis incidence, transmission risk especially in aircrafts has become an important issue worldwide. But in many of the studies conducted in this regard, transmission of tuberculosis in aircrafts was found very low...
January 2018: Mikrobiyoloji Bülteni
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