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https://www.readbyqxmd.com/read/29793125/assessment-of-cam-morphology-of-the-hip-with-ultra-sound-imaging-by-physical-therapists-is-reliable-and-valid
#1
Liliane Mandema, Igor Tak, Brigitte Mooij, Ruud Groeneweg, Eric Bakker, Peter van Veldhoven
BACKGROUND: Femoroacetabular impingement syndrome is considered present when a combination of clinical symptoms, signs and morphological features of the hip co-exist. Imaging is increasingly popular in primary health care settings with physical therapists performing ultrasound (US). OBJECTIVE: The aim of this study was to assess the diagnostic accuracy and inter-rater reliability of ultrasound in determining presence of cam morphology. DESIGN: A concurrent validity and inter-rater reliability study...
April 14, 2018: Physical Therapy in Sport
https://www.readbyqxmd.com/read/29793009/evidence-on-the-measurement-properties-of-health-related-quality-of-life-instruments-is-largely-missing-in-patients-with-low-back-pain-a-systematic-review
#2
REVIEW
Alessandro Chiarotto, Caroline B Terwee, Steven J Kamper, Maarten Boers, Raymond W Ostelo
OBJECTIVE: To synthesize the measurement properties of five health-related quality of life (HRQoL) instruments [Short Form 36 (SF-36), Short Form 12 (SF-12), EuroQol 5D (EQ-5D), Nottingham Health Profile (NHP), PROMIS Global Health (PROMIS-GH-10)] in patients with low back pain (LBP). STUDY DESIGN AND SETTING: Six electronic databases (MEDLINE, EMBASE, CINAHL, PsycINFO, SportDiscus, Google Scholar) were searched (July 2017). Studies assessing any measurement property in non-specific LBP patients were included...
May 21, 2018: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/29792967/validated-reverse-phase-hplc-diode-array-method-for-the-quantification-of-intact-bevacizumab-infliximab-and-trastuzumab-for-long-term-stability-study
#3
Antonio Martínez-Ortega, Agustín Herrera, Antonio Salmerón-García, José Cabeza, Luis Cuadros-Rodríguez, Natalia Navas
The aim of the present study was to develop suitable and reliable method for quantification three of the most worldwide used therapeutic monoclonal antibodies (mAbs) -bevazizumab (BVZ), infliximab (INF) and trastuzumab (TTZ)- to be used in long-term stability studies. Reverse phase (RP) was selected by its greater sensibility and reproducibility comparing with other chromatographic modes. Then a high performance liquid chromatography with diode array detection (RP)HPLC/DAD method was checked. Since the three mAbs represent the active ingredient in the medicines in which they are formulated, the selected method was validated for each one in accordance with the International Conference on Harmonization (ICH) guidelines for pharmaceuticals for human use...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792942/optimisation-of-decontamination-method-and-influence-of-culture-media-on-the-recovery-of-mycobacterium-avium-subspecies-paratuberculosis-from-spiked-water-sediments
#4
G Aboagye, M T Rowe
The recovery of Mycobacterium avium subspecies paratuberculosis (Map) from the environment can be a laborious process - owing to Map being fastidious, its low number, and also high numbers of other microbial populations in such settings. Protocols i.e. filtration, decontamination and modified elution were devised to recover Map from spiked water sediments. Three culture media: Herrold's Egg Yolk Media (HEYM), Middlebrook 7H10 (M-7H10) and Bactec 12B were then employed to grow the organism following its elution...
May 21, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29792937/a-reliable-targeted-next-generation-sequencing-strategy-for-diagnosis-of-myopathies-and-muscular-dystrophies-especially-for-the-giant-titin-and-nebulin-genes
#5
Reda Zenagui, Delphine Lacourt, Henri Pegeot, Kevin Yauy, Raul Juntas Morales, Corine Theze, François Rivier, Claude Cances, Guilhem Sole, Dimitri Renard, Ulrike Walther-Louvier, Xavier Ferrer-Monasterio, Caroline Espil, Marie-Christine Arné-Bes, Pascal Cintas, Emmanuelle Uro-Coste, Marie-Laure Martin Negrier, Valérie Rigau, Eric Bieth, Cyril Goizet, Mireille Claustres, Michel Koenig, Mireille Cossée
Myopathies and muscular dystrophies (M-MDs) are genetically heterogeneous diseases, with more than 100 identified genes, including the giant and complex titin (TTN) and nebulin (NEB) genes. Next-generation sequencing technology revolutionized M-MD diagnosis and revealed high frequency of TTN and NEB variants. We developed a next-generation sequencing diagnostic strategy targeted on the coding sequences of 135 M-MD genes. Comparison of two targeted capture technologies (SeqCap EZ Choice library capture kit (Roche-Nimblegen) and Nextera Rapid Capture Custom Enrichment kit (Illumina)) and of two whole exome sequencing kits (SureSelect V5 (Agilent) and TruSeq RapidExome capture (Illumina)) revealed best coverage with the SeqCap EZ Choice protocol...
May 21, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29792459/teamwork-in-healthcare-key-discoveries-enabling-safer-high-quality-care
#6
Michael A Rosen, Deborah DiazGranados, Aaron S Dietz, Lauren E Benishek, David Thompson, Peter J Pronovost, Sallie J Weaver
Few industries match the scale of health care. In the United States alone, an estimated 85% of the population has at least 1 health care encounter annually and at least one quarter of these people experience 4 to 9 encounters annually. A single visit requires collaboration among a multidisciplinary group of clinicians, administrative staff, patients, and their loved ones. Multiple visits often occur across different clinicians working in different organizations. Ineffective care coordination and the underlying suboptimal teamwork processes are a public health issue...
May 2018: American Psychologist
https://www.readbyqxmd.com/read/29792422/toward-total-synthesis-of-thiolate-protected-metal-nanoclusters
#7
Qiaofeng Yao, Tiankai Chen, Xun Yuan, Jianping Xie
Total synthesis, where desired organic- and/or biomolecules could be produced from simple precursors at atomic precision and with known step-by-step reactions, has prompted centuries-lasting bloom of organic chemistry since its conceptualization in 1828 (Wöhler synthesis of urea). Such expressive science is also highly desirable in nanoscience, since it represents a decisive step toward atom-by-atom customization of nanomaterials for basic and applied research. Although total synthesis chemistry is less established in nanoscience, recent years have witnessed seminal advances and increasing research efforts devoted into this field...
May 24, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29792243/understanding-the-science-of-portion-control-and-the-art-of-downsizing
#8
Marion M Hetherington, Pam Blundell-Birtill, Samantha J Caton, Joanne E Cecil, Charlotte E Evans, Barbara J Rolls, Tang Tang
Offering large portions of high-energy-dense (HED) foods increases overall intake in children and adults. This is known as the portion size effect (PSE). It is robust, reliable and enduring. Over time, the PSE may facilitate overeating and ultimately positive energy balance. Therefore, it is important to understand what drives the PSE and what might be done to counter the effects of an environment promoting large portions, especially in children. Explanations for the PSE are many and diverse, ranging from consumer error in estimating portion size to simple heuristics such as cleaning the plate or eating in accordance with consumption norms...
May 24, 2018: Proceedings of the Nutrition Society
https://www.readbyqxmd.com/read/29792197/antimicrobial-susceptibility-of-campylobacter-jejuni-and-campylobacter-coli-comparison-between-etest-and-a-broth-dilution-method
#9
Maya Azrad, Linda Tkhawkho, Natalia Isakovich, Orna Nitzan, Avi Peretz
BACKGROUND: Campylobacter is a leading cause of foodborne gasteroenteritis worldwide. Antimicrobial susceptibility testing for Campylobacter spp. is not routinely performed by most clinical laboratories. However, the emergence of resistant isolates strengthens the importance of antimicrobial susceptibility testing and the critical need for epidemiologic surveillance. The aim of this study was to compare the efficacy of Etest and Sensititre kit (a broth microdilution method) as methods for susceptibility tests and the critical need for epidemiologic surveillance...
May 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29792046/annals-express-fast-screening-of-n-glycosylation-disorders-by-sialotransferrin-profiling-with-capillary-zone-electrophoresis
#10
Hermi A Kingma, Fjodor H Van der Sluijs, Rebecca M Heiner-Fokkema
BACKGROUND: Congenital disorders of glycosylation (CDG) are a growing group of rare genetic disorders. The most frequently used screening method is sialotransferrin profiling using isoelectric focussing (IEF). Capillary zone electrophoresis (CZE) may be a simple and fast alternative. We investigated the Capillarys ¬T M CDT assay (Sebia, France) to screen for N-glycosylation disorders, using IEF as gold standard. METHODS: Intra- and interassay precision were established, and analyses in heparin-anticoagulated plasma and serum were compared...
January 1, 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29791964/ultrasound-guided-treatment-of-peripheral-nerve-pathology
#11
Nathan Dettori, Hema Choudur, Avneesh Chhabra
High-resolution ultrasound serves as a fast, accessible, reliable, and radiation-free tool for anatomical and dynamic evaluation of various peripheral nerves. It can be used not only to identify and diagnose peripheral nerve and perineural pathology accurately but also to guide various nerve and perineural interventions. We describe the normal and pathologic appearances of peripheral nerves, the pathologies commonly affecting the individual peripheral nerves, and the current ultrasound-guided peripheral nerve interventions and techniques...
July 2018: Seminars in Musculoskeletal Radiology
https://www.readbyqxmd.com/read/29791726/building-capacity-in-health-and-education-systems-to-deliver-interventions-that-strengthen-early-child-development
#12
Milagros Nores, Camila Fernandez
Building capacity within health and education systems of low- and middle-income countries in order to deliver high-quality early childhood services requires coordinated efforts across sectors, effective governance, sufficient funding, an adequate workforce, reliable data systems, and continuous monitoring, evaluation, and improvement cycles; it also requires partnerships with the private sector, communities, and parents. In addition, building capacity requires leadership, innovation of strategies to fit into existing structures, evidence-based intervention models, and effective partnerships that help make interventions more culturally relevant, help finance them, and help create institutional long-term support and sustainability for them...
May 2018: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/29791515/comparison-of-oscillometric-doppler-and-invasive-blood-pressure-measurement-in-anesthetized-goats
#13
Olga Szaluś-Jordanow, Michał Czopowicz, Agata Moroz, Marcin Mickiewicz, Magdalena Garncarz, Emilia Bagnicka, Tadeusz Frymus, Jarosław Kaba
Arterial blood pressure (BP) can be measured directly using an invasive intra-arterial method. This method is considered a gold standard, however it is potentially hazardous and requires expensive equipment and professional skills. Therefore, two non-invasive methods-Doppler ultrasonic sphygmomanometry and oscillometry-have been introduced in veterinary medicine. Their accuracy has so far been reliably evaluated in various animal species, however only one study included a small group of goats. Therefore, we carried out a large-scale study which aimed to evaluate agreement between the two non-invasive methods and invasive intra-arterial BP measurement in anesthetized goats at various age...
2018: PloS One
https://www.readbyqxmd.com/read/29791452/validity-and-reliability-of-two-alternate-versions-of-the-montreal-cognitive-assessment-hong-kong-version-for-screening-of-mild-neurocognitive-disorder
#14
Adrian Wong, Stanley Yiu, Ziad Nasreddine, Kam-Tat Leung, Alexander Lau, Yannie O Y Soo, Lawrence Ka-Sing Wong, Vincent Mok
OBJECTIVE: Repeated testing using the Montreal Cognitive Assessment (MoCA) increases risks for practice effects which may bias measurements of cognitive change. The objective of this study is to develop two alternate versions of the MoCA (Hong Kong version; HK-MoCA) and to investigate the validity and reliability of the alternate versions in patients with DSM-5 Mild Neurocognitive Disorder (Mild NCD) and cognitively healthy controls. METHODS: Concurrent validity and inter-scale agreement were examined by Pearson correlation of the total scores between the original and alternate versions and the Bland-Altman Method...
2018: PloS One
https://www.readbyqxmd.com/read/29791443/traceability-reproducibility-and-wiki-exploration-for-%C3%A3-la-carte-reconstructions-of-genome-scale-metabolic-models
#15
Méziane Aite, Marie Chevallier, Clémence Frioux, Camille Trottier, Jeanne Got, María Paz Cortés, Sebastián N Mendoza, Grégory Carrier, Olivier Dameron, Nicolas Guillaudeux, Mauricio Latorre, Nicolás Loira, Gabriel V Markov, Alejandro Maass, Anne Siegel
Genome-scale metabolic models have become the tool of choice for the global analysis of microorganism metabolism, and their reconstruction has attained high standards of quality and reliability. Improvements in this area have been accompanied by the development of some major platforms and databases, and an explosion of individual bioinformatics methods. Consequently, many recent models result from "à la carte" pipelines, combining the use of platforms, individual tools and biological expertise to enhance the quality of the reconstruction...
May 23, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29791408/correction-of-phase-delay-distortion-for-%C3%AE-%C3%AE-circular-scanning
#16
Jian Liu, Xiaoyu You, Yuhang Wang, Chenguang Liu, Jiubin Tan
α-β circular scanning with a large scanning field of view and high reliability can be widely applied in laser scanning imaging systems and laser processing. However, mechanical inertia of the galvanometers introduces phase delay and ultimately leads to scanning distortions in α-β circular scanning in both constant angular velocity scanning (CAVS) and constant line velocity scanning (CLVS). To compensate for the phase-delay distortions, two correction models are respectively proposed for CAVS and CLVS, which utilize phase-frequency relationships based on the galvanometer's transfer function...
May 20, 2018: Applied Optics
https://www.readbyqxmd.com/read/29791401/investigation-of-index-change-in-compression-molding-of-as-40-se-50-s-10-chalcogenide-glass
#17
Lin Zhang, Wenchen Zhou, Neil J Naples, Allen Y Yi
Chalcogenide glasses are emerging as alternative materials for low-cost and high-volume glass molding processes for infrared optics. In precision glass molding, it is well documented that the refractive index variation in the molded elements can lead to substantial amounts of aberrations. The variation has such a significant effect that the optical designs with molded lenses need to be carefully considered and compensated for index variation to achieve targeted optical performance. This research is aimed to evaluate the refractive index change of a chalcogenide glass during the molding process by both finite element method-based simulation and optical experiment...
May 20, 2018: Applied Optics
https://www.readbyqxmd.com/read/29791066/application-of-denaturing-capillary-electrophoresis-for-the-detection-of-prognostic-mutations-in-isocitrate-dehydrogenase-1-and-isocitrate-dehydrogenase-2-genes-in-brain-tumors
#18
Lucie Benesova, Barbora Belsanova, Filip Kramar, Tereza Halkova, Vladimir Benes, Marek Minarik
Malignant transformation in gliomas is frequently supplemented by somatic mutations in isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 genes. It has recently emerged that mutations in these genes are associated with prolonged survival and should be used as prognostic factor in management of brain cancer patients. There are several approaches in use for the detection of isocitrate dehydrogenase 1 and 2 mutations, however, these often exhibit shortcomings such as convoluted protocols with long processing time, complex (and costly) dedicated fluorescent probes and/or high demand on amounts of input DNA...
May 23, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29790753/target-decoy-based-false-discovery-rate-estimation-for-large-scale-metabolite-identification
#19
Xusheng Wang, Drew R Jones, Timothy I Shaw, Ji-Hoon Cho, Yuanyuan Wang, Haiyan Tan, Boer Xie, Suiping Zhou, Yuxin Li, Junmin Peng
Metabolite identification is a crucial step in mass spectrometry (MS)-based metabolomics. However, it is still challenging to assess the confidence of assigned metabolites. In this study, we report a novel method for estimating false discovery rate (FDR) of metabolite assignment with a target-decoy strategy, in which the decoys are generated through violating the octet rule of chemistry by adding small odd numbers of hydrogen atoms. The target-decoy strategy was integrated into JUMPm, an automated metabolite identification pipeline for large-scale MS analysis, and was also evaluated with two other metabolomics tools, mzMatch and mzMine 2...
May 23, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29790741/entrapment-of-prostate-cancer-circulating-tumor-cells-with-a-sequential-size-based-microfluidic-chip
#20
Xiang Ren, Brittni Foster, Parham Ghassemi, Jeannine S Strobl, Bethany A Kerr, Masoud Agah
Circulating tumor cells (CTCs) are broadly accepted as an indicator for early cancer diagnosis and disease severity. However, there is currently no reliable method available to capture and enumerate all CTCs as most systems require either an initial CTC isolation or antibody-based capture for CTC enumeration. Many size-based CTC detection and isolation microfluidic platforms were presented in the past few years. Here we describe a new size-based, multiple-row cancer cell entrapment device that captured LNCaP-C4-2 prostate cancer cells with >95% efficiency when in spiked mouse whole blood at ~50 cells/mL...
May 23, 2018: Analytical Chemistry
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