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Longfa Jiang, Shengjun Wu
This study aims to investigate the effect of pullulan on the denaturation of the myofibrillar protein of grass carp (Ctenopharyngodon idella) during frozen storage at -18 °C for 30 days. Pullulan or commercial cryoprotectant (1 g dry weight) were added to 100 g of the myofibrillar protein of grass carp (C. idella), respectively, which was frozenly stored at -18 °C for 30 days, and the changes in the state of unfrozen of water, solubility, Ca 2+ -adenylpyrophosphatase (ATP ase ) activity, and sulfhydryl content of myofibrils were monitored during frozen storage...
February 14, 2018: International Journal of Biological Macromolecules
Andrea Frustaci, Alessandro De Luca, Valentina Guida, Tommaso Biagini, Tommaso Mazza, Carlo Gaudio, Claudio Letizia, Matteo Antonio Russo, Nicola Galea, Cristina Chimenti
BACKGROUND: Mutations of α-actin gene (ACTC1) have been phenotypically related to various cardiac anomalies, including hypertrophic cardiomyopathy and dilated cardiomyopathy and left ventricular (LV) myocardial noncompaction. A novel ACTC mutation is reported as cosegregating for familial hypertrophic cardiomyopathy and LV myocardial noncompaction with transmural crypts. METHODS AND RESULTS: In an Italian family of 7 subjects, 4 aged 10 (II-1), 14 (II-2), 43 (I-4) and 46 years (I-5), presenting abnormal ECG changes, dyspnea and palpitation (II-2, I-4, and I-5), and recurrent cerebral ischemic attack (I-5), underwent 2-dimensional echo, cardiac magnetic resonance, Holter monitoring, and next-generation sequencing gene analysis...
February 10, 2018: Journal of the American Heart Association
Tao Zhang, Xiaoqi Xu, Zhaojie Li, Yuming Wang, Yong Xue, Changhu Xue
In the present work, we provide insight into electrostatic interactions and phase behaviors in mixtures of myofibrillar protein (MP) and κ-carrageenan (KC) of various pHs (8.0-3.0) and biopolymer weight ratios [R, from 1:1 to 20:1; total concentration=0.05% (w/w)] through turbidimetric analysis, dynamic light scattering (DLS) and zeta potential analysis, and optical microscopy. At R=1: 1, critical pH values (i.e., pHc, pHΦ1, and pHΦ2), which indicate phase transitions interrelated to the formation of soluble or insoluble MP-KC complexes, were observed at pH7...
March 2018: Food Research International
A Zawadzki, C Alloo, A B Grossi, E S P do Nascimento, L C Almeida, S Bogusz Junior, L H Skibsted, D R Cardoso
Addition of β-acids extracted from hop at different levels (0, 30mgkg-1, 60mgkg-1, 240mgkg-1) to the diet of broiler chickens demonstrated significant effects on the average concentration of polar metabolites and fatty acids of relevance for meat quality. The largest metabolic differences between control group and chicken fed different levels of β-acids were achieved using 30mgkg-1 of supplement. As determined by EPR spin-trapping, increased redox stability was also obtained for meat from chicken fed 30mgkg-1 of β-acids which also had highest level of endogenous antioxidants, especially anserine, carnosine, NADH and PUFAs...
March 2018: Food Research International
Tingyang Zhou, Evan R Prather, Davis E Garrison, Li Zuo
Ischemia reperfusion (IR), present in myocardial infarction or extremity injuries, is a major clinical issue and leads to substantial tissue damage. Molecular mechanisms underlying IR injury in striated muscles involve the production of reactive oxygen species (ROS). Excessive ROS accumulation results in cellular oxidative stress, mitochondrial dysfunction, and initiation of cell death by activation of the mitochondrial permeability transition pore. Elevated ROS levels can also decrease myofibrillar Ca2+ sensitivity, thereby compromising muscle contractile function...
January 31, 2018: International Journal of Molecular Sciences
Xiao-Ting Xuan, Yan Cui, Xu-Dong Lin, Jing-Feng Yu, Xiao-Jun Liao, Jian-Gang Ling, Hai-Tao Shang
The effects of high hydrostatic pressure (HHP) treatments (200, 300, and 400 MPa for 1, 3, 5 and 10 min) on the shelling efficacy (the rate of shelling, the rate of integrity and yield of razor clam meat) and the physicochemical (drip loss, water-holding capacity, pH, conductivity, lipid oxidation, Ca2+ -ATPase activity, myofibrillar protein content), microbiological (total viable counts) and microstructural properties of fresh razor clam (Sinonovacula constricta) were investigated. HHP treatments significantly (P < 0...
January 22, 2018: Journal of Food Science
Paulo G Gandra, Amy A Shiah, Leonardo Nogueira, Michael C Hogan
Skeletal muscle can develop a prolonged low frequency-stimulation force depression (PLFFD) following fatigue-inducing contractions. Increased levels of reactive oxygen species (ROS) have been implicated in the development of PLFFD. During exercise the skeletal muscle intracellular PO2 decreases to relatively low levels, and can be further decreased when there is an impairment in O2 diffusion or availability, such as in certain chronic diseases and during exercise at high altitude. Since ROS generation by mitochondria is elevated at very low PO2 in cells, we tested the hypothesis that treatment of muscle fibres with a mitochondrial-targeted antioxidant at a very low, near hypoxic PO2 , can attenuate PLFFD...
January 15, 2018: Journal of Physiology
Christoph Paone, Steven Rudeck, Christelle Etard, Uwe Strähle, Wolfgang Rottbauer, Steffen Just
Sarcomeric protein turnover needs to be tightly balanced to assure proper assembly and renewal of sarcomeric units within muscle tissues. The mechanisms regulating these fundamental processes are only poorly understood, but of great clinical importance since many cardiac and skeletal muscle diseases are associated with defective sarcomeric organization. The SET- and MYND domain containing protein 1b (Smyd1b) is known to play a crucial role in myofibrillogenesis by functionally interacting with the myosin chaperones Unc45b and Hsp90α1...
January 10, 2018: Biochemical and Biophysical Research Communications
Chang-Yu Zhou, Dao-Dong Pan, Yang-Ying Sun, Chun-Bao Li, Xing-Lian Xu, Jin-Xuan Cao, Guang-Hong Zhou
BACKGROUND: In order to evaluate the effect of cooking temperatures on the nutrition quality of dry-cured hams, 60 biceps femoris samples from 16 Jinhua hams were divided into four groups (the control, 70, 100 and 120 °C) and cooked for 30 min. Carbonyls content, sulfhydryl groups, surface hydrophobicity, microstructure, proteins aggregation and digestibility of myofibrillar proteins were investigated. RESULTS: Cooking promoted the carbonylation and decreased sulfhydryl groups in temperatures-dependent ways...
January 9, 2018: Journal of the Science of Food and Agriculture
Sarah J Brockmann, Axel Freischmidt, Patrick Oeckl, Kathrin Müller, Srinivas K Ponna, Anika M Helferich, Christoph Paone, Jörg Reinders, Kerstin Kojer, Michael Orth, Manu Jokela, Mari Auranen, Bjarne Udd, Andreas Hermann, Karin M Danzer, Peter Lichtner, Paul Walther, Albert C Ludolph, Peter M Andersen, Markus Otto, Petri Kursula, Steffen Just, Jochen H Weishaupt
Mutations in the mitochondrially located protein CHCHD10 cause motoneuron disease by an unknown mechanism. In this study, we investigate the mutations p.R15L and p.G66V in comparison to wild-type CHCHD10 and the non-pathogenic variant p.P34S in vitro, in patient cells as well as in the vertebrate in vivo model zebrafish. We demonstrate a reduction of CHCHD10 protein levels in p.R15L and p.G66V mutant patient cells to approximately 50%. Quantitative real-time PCR revealed that expression of CHCHD10 p.R15L, but not of CHCHD10 p...
January 5, 2018: Human Molecular Genetics
Adam Eckhardt, Lucie Kulhava, Ivan Miksik, Statis Pataridis, Marketa Hlavackova, Jana Vasinova, Frantisek Kolar, David Sedmera, Bohuslav Ostadal
The heart is characterized by a remarkable degree of heterogeneity. Since different cardiac pathologies affect different cardiac regions, it is important to understand molecular mechanisms by which these parts respond to pathological stimuli. In addition to already described left ventricular (LV)/right ventricular (RV) and transmural differences, possible baso-apical heterogeneity has to be taken into consideration. The aim of our study has been, therefore, to compare proteomes in the apical and basal parts of the rat RV and LV...
January 4, 2018: Molecular and Cellular Biochemistry
Joshua Mayourian, Delaine K Ceholski, David M Gonzalez, Timothy J Cashman, Susmita Sahoo, Roger J Hajjar, Kevin D Costa
Cardiac excitation-contraction coupling (ECC) is the orchestrated process of initial myocyte electrical excitation, which leads to calcium entry, intracellular trafficking, and subsequent sarcomere shortening and myofibrillar contraction. Neurohumoral β-adrenergic signaling is a well-established mediator of ECC; other signaling mechanisms, such as paracrine signaling, have also demonstrated significant impact on ECC but are less well understood. For example, resident heart endothelial cells are well-known physiological paracrine modulators of cardiac myocyte ECC mainly via NO and endothelin-1...
January 5, 2018: Circulation Research
Anran Wang, Dacheng Kang, Wangang Zhang, Chaoyang Zhang, Yunhe Zou, Guanghong Zhou
In this investigation, samples were treated by ultrasonic probe (20 kHz) at an intensity of 25 W cm-2 for 20 or 40 min, followed by aging at 4 °C for 1, 3 and 7 d. Ultrasound treatment significantly increased myofibrillar fragmentation index (MFI) and decreased Warner-Bratzler shear force (WBSF) at 3 and 7 d of postmortem aging (P < .05). Ultrasound-treated samples showed significantly greater intensities of autolyzed 76 kDa subunits and the lower intensity of intact 80 kDa form compared to control at 1 d of storage (P < ...
April 15, 2018: Food Chemistry
Yaolan Ma, Shanbai Xiong, Juan You, Yang Hu, Qilin Huang, Tao Yin
Physicochemical and gelation properties of myofibrillar proteins from silver carp surimi as affected by chopping under different vacuum degrees (0, 0.01, 0.02, 0.04, 0.06 and 0.08 MPa) were investigated. With the increase of vacuum degree, size and quantity of air bubbles in surimi paste decreased, disulfide bond content of myofibrillar proteins decreased significantly (p < .05) and then slight increased (p > .05), while surface hydrophobicity of myofibrillar proteins increased gradually (p < ...
April 15, 2018: Food Chemistry
Yungang Cao, Nasi Ai, Alma D True, Youling L Xiong
The dose-dependent effects of (-)-epigallocatechin-3-gallate (EGCG; at 0, 50, 100, 200, 500, and 1000 mg/L) on the physical, chemical, and oxidative stability of porcine myofibrillar protein (MP)-soybean oil emulsion systems were investigated. The results showed EGCG at all levels effectively suppressed lipid oxidation in MP emulsion composite gels during the entire chill storage (at 4 °C for 0, 3, or 7 days). The incorporation of EGCG at higher concentrations (>100 mg/L) promoted the loss of sulfhydryls, reduction of surface hydrophobicity, and aggregation and cross-linking of MP...
April 15, 2018: Food Chemistry
Bin Zhang, Chuan-Dong Fang, Gui-Juan Hao, Yang-Yang Zhang
Protein oxidation during chilling and frozen storage has recently attracted great attention due to its consequences on protein solubility and functionality. In this study, the effects of kappa-carrageenan oligosaccharides on myofibrillar protein oxidation in peeled shrimp (Litopenaeus vannamei) during frozen storage were investigated by assessing the content of the carbonyls, total sulfhydryl, dityrosine, and surface hydrophobicity. We also examined the means of antioxidant activity in vitro, degradation of muscle proteins, and integrity of tissue structure...
April 15, 2018: Food Chemistry
Zheng Li, Meng Li, Manting Du, Qingwu W Shen, Dequan Zhang
Protein degradation is primarily responsible for postmortem meat tenderization, which might be affected by phosphorylation. The objective of this study was to investigate the effect of phosphorylation on myofibrillar proteins degradation in muscle during postmortem. Here we modulated the phosphorylation status of protein by protein kinase inhibitor and phosphatase inhibitor, and the effect of these inhibitors on myofibrillar protein degradation was evaluated. Generally, myofibril fragmentation index of samples with lower phosphorylation level was higher...
April 15, 2018: Food Chemistry
S Mancini, I Medina, V Iaconisi, F Gai, A Basto, G Parisi
The present research studied the effect of a dietary inclusion with Hermetia illucens larvae meal (Hi) on rainbow trout's fillets chemical composition. The effect of Hi inclusion in diets on rainbow trout chemical characteristics was evaluated. Trout were fed three different diets: control (C, no Hi inclusion), 25% and 50% of substitution of fish meal with Hi (Hi25 and Hi50, respectively). Fillets were analysed to quantify proximate composition, carbohydrates percentage, colour parameters, nucleotides concentration, fatty acids profile, volatile organic compounds (VOCs) and myofibrillar and sarcoplasmic concentrations...
December 28, 2017: Animal: An International Journal of Animal Bioscience
Yanjun Cui, Yue Hao, Jielei Li, Yanli Gao, Xianhong Gu
BACKGROUND: Heat stress (HS) has an adverse effect on meat quality; yet, the underlying molecular mechanisms altering meat quality due to muscle responses to stress remain unclear. Sixteen castrated male crossbreeds between Landrace × Yorkshire sows and Duroc boars (79.00 ± 1.50 kg body weight) were exposed to either thermal neutral (22 °C, n=8) or heat stress (30 °C, n=8) conditions for 3 weeks. Subsequently, the longissimus dorsi (LD) muscle of all pigs was assayed for meat quality parameters and proteome analysis...
December 14, 2017: Journal of the Science of Food and Agriculture
Lili Wang, Kyungsoo Kim, Shan Parikh, Adrian Gabriel Cadar, Kevin R Bersell, Huan He, Jose R Pinto, Dmytro O Kryshtal, Bjorn C Knollmann
BACKGROUND: Mutations in cardiac troponin T (TnT) are linked to increased risk of ventricular arrhythmia and sudden death despite causing little to no cardiac hypertrophy. Studies in mice suggest that the hypertrophic cardiomyopathy (HCM)-associated TnT-I79N mutation increases myofilament Ca sensitivity and is arrhythmogenic, but whether findings from mice translate to human cardiomyocyte electrophysiology is not known. OBJECTIVES: To study the effects of the TnT-I79N mutation in human cardiomyocytes...
December 4, 2017: Journal of Molecular and Cellular Cardiology
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