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Myofibrillar

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https://www.readbyqxmd.com/read/28530580/offgel-electrophoresis-and-tandem-mass-spectrometry-approach-compared-with-dna-based-pcr-method-for-authentication-of-meat-species-from-raw-and-cooked-ground-meat-mixtures-containing-cattle-meat-water-buffalo-meat-and-sheep-meat
#1
Basappa M Naveena, Deepak S Jagadeesh, A Jagadeesh Babu, T Madhava Rao, Veeranna Kamuni, S Vaithiyanathan, Vinayak V Kulkarni, Srikanth Rapole
The present study compared the accuracy of an OFFGEL electrophoresis and tandem mass spectrometry-based proteomic approach with a DNA-based method for meat species identification from raw and cooked ground meat mixes containing cattle, water buffalo and sheep meat. The proteomic approach involved the separation of myofibrillar proteins using OFFGEL electrophoresis, SDS-PAGE and protein identification by MALDI-TOF MS. Species-specific peptides derived from myosin light chain-1 and 2 were identified for authenticating buffalo meat spiked at a minimum 0...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28528595/implications-of-white-striping-and-spaghetti-meat-abnormalities-on-meat-quality-and-histological-features-in-broilers
#2
G Baldi, F Soglia, M Mazzoni, F Sirri, L Canonico, E Babini, L Laghi, C Cavani, M Petracci
During the past few years, there has been an increasing prevalence of broiler breast muscle abnormalities, such as white striping (WS) and wooden breast conditions. More recently, a new muscular abnormality termed as spaghetti meat (SM) because of the altered structural integrity of the Pectoralis major muscle often associated with WS has emerged. Thus, this study aimed at evaluating the effects of WS and SM conditions, occurring alone or combined within the same P. major muscle, on meat quality traits and muscle histology...
May 22, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28523323/discovery-of-a-new-mutation-in-the-desmin-gene-in-a-young-patient-with-cardiomyopathy-and-muscular-weakness
#3
Ruxandra Oana JurcuŢ, Alexandra Eugenia Bastian, Sebastian Militaru, Aura Popa, Emilia Manole, Bogdan Alexandru Popescu, Jonna Tallila, Bogdan Ovidiu Popescu, Carmen Doina Ginghină
A 25-year-old woman with a five years history of syncope, mild left ventricular hypertrophy and moderately enlarged atria, was diagnosed with third degree atrioventricular heart block alternating with atrioventricular heart block 2:1, and received a dual chamber pacemaker. After three years of evolution, she developed atrial fibrillation, marked biatrial enlargement, severely depressed longitudinal myocardial velocities, associated with mild girdle weakness and slight increase in creatine kinase level. The diagnosis of restrictive cardiomyopathy with mild skeletal myopathy imposed the screening for a common etiology...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28516388/effects-of-different-selenium-sources-on-growth-performance-antioxidant-capacity-and-meat-quality-of-local-chinese-subei-chickens
#4
J L Li, L Zhang, Z Y Yang, Z Y Zhang, Y Jiang, F Gao, G H Zhou
Despite increasing evidence indicating the essential involvement of selenium (Se) on growth performance, antioxidant capacity, and meat quality of commercial broilers, the effects of different Se sources on local Chinese Subei chickens is unclear. A total of 360 50-day-old male chickens were individually weighed and randomly allocated to four treatment groups. Chickens in each of the four groups were fed diets supplemented with 0.3 mg Se/kg as sodium Se (SS), Se-enriched yeast (SY), selenomethionine (Met-Se), or nano red element Se (Nano-Se) for 40 days...
May 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28515855/speckle-tracking-echocardiography-to-assess-regional-ventricular-function-in-patients-with-apical-hypertrophic-cardiomyopathy
#5
María Cristina Saccheri, Tomás Francisco Cianciulli, Luis Alberto Morita, Ricardo José Méndez, Martín Alejandro Beck, Juan Enrique Guerra, Alberto Cozzarin, Luciana Jimena Puente, Lorena Romina Balletti, Jorge Alberto Lax
AIM: To explore regional systolic strain of midwall and endocardial segments using speckle tracking echocardiography in patients with apical hypertrophic cardiomyopathy (HCM). METHODS: We prospectively assessed 20 patients (mean age 53 ± 16 years, range: 18-81 years, 10 were male), with apical HCM. We measured global longitudinal peak systolic strain (GLPSS) in the midwall and endocardium of the left ventricle. RESULTS: The diastolic thickness of the 4 apical segments was 16...
April 26, 2017: World Journal of Cardiology
https://www.readbyqxmd.com/read/28510117/novex-3-the-tiny-titin-of-muscle
#6
REVIEW
Dalma Kellermayer, John E Smith, Henk Granzier
The giant multi-functional striated muscle protein titin is the third most abundant muscle protein after myosin and actin. Titin plays a pivotal role in myocardial passive stiffness, structural integrity and stress-initiated signaling pathways. The complete sequence of the human titin gene contains three isoform-specific mutually exclusive exons [termed novel exons (novex)] coding for the I-band sequence, named novex-1 (exon 45), novex-2 (exon 46) and novex-3 (exon 48). Transcripts containing either the novex-1 or novex-2 exons code for the novex-1 and novex-2 titin isoforms...
April 7, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28502773/phosphorylation-of-%C3%AE-b-crystallin-in-the-myocardium-analysis-of-relations-with-aging-and-cardiomyopathy
#7
Natalia A Muraleva, Vasiliy A Devyatkin, Nataliya G Kolosova
Phosphorylation is a major post-translational modification of αB-crystallin (CryaB) and determines this protein's chaperone activity, intracellular distribution, translocation, and cytoprotective functions. Phosphorylation of CryaB manifests itself as either beneficial or deleterious consequences depending on the extent of phosphorylation and interaction with the cytoskeleton. Herein, for the first time, we compared the age-related alterations of the expression and phosphorylation (on Ser59: pS59) of CryaB in the myocardium of Wistar and senescence-accelerated OXYS rats...
May 11, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28488683/progressive-hereditary-spastic-paraplegia-caused-by-a-homozygous-ky-mutation
#8
Yuval Yogev, Yonatan Perez, Iris Noyman, Anwar Abu Madegem, Hagit Flusser, Zamir Shorer, Eugene Cohen, Leonid Kachko, Analia Michaelovsky, Ruth Birk, Arie Koifman, Max Drabkin, Ohad Wormser, Daniel Halperin, Rotem Kadir, Ohad S Birk
Twelve individuals of consanguineous Bedouin kindred presented with autosomal recessive progressive spastic paraplegia evident as of age 0-24 months, with spasticity of lower limbs, hyperreflexia, toe walking and equinus deformity. Kyphoscolisois was evident in older patients. Most had atrophy of the lateral aspects of the tongue and few had intellectual disability. Nerve conduction velocity, electromyography and head and spinal cord magnetic resonance imaging were normal in tested subjects. Muscle biopsy showed occasional central nuclei and fiber size variability with small angular fibers...
May 10, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28482234/proteomic-approach-to-investigate-the-impact-of-different-dietary-supplementation-on-lamb-meat-tenderness
#9
Antonella Della Malva, Rosaria Marino, Antonella Santillo, Giovanni Annicchiarico, Mariangela Caroprese, Agostino Sevi, Marzia Albenzio
The aim of this study was to evaluate the effect of dietary supplementation of linseed and/or quinoa on tenderness and on proteome of lamb meat. Thirty-two Italian Merino lambs were distributed into 4 groups with different diet: control (CO) with no supplemental fat, linseed (LS), quinoa (QS) and QS+LS diets. Meat obtained by lamb fed linseed showed the lowest values of WBSF (P<0.001), hardness (P<0.01), gumminess (P<0.01) and chewiness (P<0.01). Proteomic changes of myofibrillar and sarcoplasmic proteins were estimated with SDS-PAGE, Western Blot and Two-Dimensional Gel Electrophoresis...
April 29, 2017: Meat Science
https://www.readbyqxmd.com/read/28469177/early-signs-of-architectural-and-biomechanical-failure-in-isolated-myofibers-and-immortalized-myoblasts-from-desmin-mutant-knock-in-mice
#10
Stefanie Diermeier, Julian Iberl, Kristina Vetter, Michael Haug, Charlotte Pollmann, Barbara Reischl, Andreas Buttgereit, Sebastian Schürmann, Marina Spörrer, Wolfgang H Goldmann, Ben Fabry, Fatiha Elhamine, Robert Stehle, Gabriele Pfitzer, Lilli Winter, Christoph S Clemen, Harald Herrmann, Rolf Schröder, Oliver Friedrich
In striated muscle, desmin intermediate filaments interlink the contractile myofibrillar apparatus with mitochondria, nuclei, and the sarcolemma. The desmin network's pivotal role in myocytes is evident since mutations in the human desmin gene cause severe myopathies and cardiomyopathies. Here, we investigated skeletal muscle pathology in myofibers and myofibrils isolated from young hetero- and homozygous R349P desmin knock-in mice, which carry the orthologue of the most frequent human desmin missense mutation R350P...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28463752/relationship-between-proteolysis-and-water-holding-of-myofibrils
#11
Zhen Zeng, Cheng Li, Per Ertbjerg
The purpose of this study was to increase the knowledge on the relationship between proteolysis of myofibrillar proteins and the water-holding of meat. Myofibrils isolated from porcine longissimus thoracis et lumborum muscle were used as a model system. Myofibrils were incubated with either calpain-2, the proteasome or a lysosomal extract at 25°C for 2h. All three proteolytic systems improved the relative water-holding and generally there was a larger effect with increasing amount of enzymes in the incubation...
April 25, 2017: Meat Science
https://www.readbyqxmd.com/read/28455288/cardiac-myofibrillar-contractile-properties-during-the-progression-from-hypertension-to-decompensated-heart-failure
#12
Laurin M Hanft, Craig A Emter, Kerry S McDonald
Heart failure arises, in part, from a constellation of changes in cardiac myocytes including remodeling, energetics, calcium handling, and myofibrillar function. However, little is known about the changes in myofibrillar contractile properties during the progression from hypertension to decompensated heart failure. The aim of this study was to provide a comprehensive assessment of myofibrillar functional properties from health to heart disease. A rodent model of uncontrolled hypertension was used to test the hypothesis that myocytes in compensated hearts exhibit increased force, higher rates of force development, faster loaded shortening, and greater power output; however, with progression to overt heart failure we predicted marked depression in these contractile properties...
April 28, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28452077/structure-and-function-of-human-muscle-fibres-and-muscle-proteome-in-physically-active-older-men
#13
Lorenza Brocca, Jamie S McPhee, Emanuela Longa, Monica Canepari, Olivier Seynnes, Giuseppe De Vito, Maria Antonietta Pellegrino, Marco Narici, Roberto Bottinelli
Contradictory results have been reported on the impact of ageing on structure and functions of skeletal muscle fibres likely due to complex interplay between ageing and other phenomena such as disuse and diseases. Here we recruited healthy, physically and socially active young (YO) and elderly (EL) men in order to study aging per se without the confounding effects of impaired physical function. In vivo analyses of quadriceps and in vitro analyses of vastus lateralis muscle biopsies were performed. In EL subjects, our results show that: (i) quadriceps volume, maximum voluntary torque (MVC), and patellar tendon force (Ft) were significantly lower; (ii) muscle fibres went through significant atrophy and impairment of specific force (Po/CSA) and unloaded shortening velocity (Vo); (iii) myosin/actin ratio and myosin content in individual muscle fibres were not altered; (iv) muscle proteome went through quantitative adaptations, namely an up-regulation of the content of several groups of proteins among which myofibrillar proteins and antioxidant defence systems; (v) muscle proteome went through qualitative adaptations, namely phosphorylation of several proteins, including Myosin Light Chain-2 slow and Troponin T and carbonylation of Myosin Heavy Chains...
April 27, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28449327/skeletal-muscle-il-15-il-15r%C3%AE-and-myofibrillar-protein-synthesis-after-resistance-exercise
#14
Alberto Pérez-López, James McKendry, Marcos Martin-Rincon, David Morales-Alamo, Bárbara Pérez-Köhler, David Valadés, Julia Buján, Jose A L Calbet, Leigh Breen
In vitro and in vivo studies described the myokine IL-15 and its receptor IL-15Rα as anabolic/anti-atrophy agents, however the protein expression of IL-15Rα has not been measured in human skeletal muscle and data regarding IL-15 expression remain inconclusive. The purpose of the study was to determine serum and skeletal muscle IL-15 and IL-15Rα responses to resistance exercise session and to analyse their association with myofibrillar protein synthesis (MPS). Fourteen participants performed a bilateral leg resistance exercise composed of 4 sets of leg press and 4 sets of knee extension at 75% 1RM to task failure...
April 27, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28447737/17%C3%AE-estradiol-protects-against-doxorubicin-induced-cardiotoxicity-in-male-sprague-dawley-rats-by-regulating-nadph-oxidase-and-apoptosis-genes
#15
Xiao-Juan Zhang, Xiao-Qing Cao, Chun-Sheng Zhang, Zhuo Zhao
Doxorubicin (DOX) is one of the most effective chemotherapeutic agents for the treatment of a number of malignancies. However, its use is limited by serious cardiotoxic effects, for which there are currently no reliable pharmacologic therapies. Estrogen has exhibited protective effects against cardiac stressors in male and female animal models; however, its effects on DOX‑induced cardiotoxicity remain unknown. High mortality and morbidity rates have been observed in patients with cancer worldwide, and DOX is often administered to a greater number of men than women...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28436997/truncating-mutations-on-myofibrillar-myopathies-causing-genes-as-prevalent-molecular-explanations-on-patients-with-dilated-cardiomyopathy
#16
Alexandre Janin, Karine N'Guyen, Gilbert Habib, Claire Dauphin, Valérie Chanavat, Patrice Bouvagnet, Romain Eschalier, Streichenberger Nathalie, Philippe Chevalier, Gilles Millat
Dilated Cardiomyopathy (DCM) is one of the leading causes of heart failure with high morbidity and mortality. More than 40 genes have been reported to cause DCM. To provide new insights into the pathophysiology of dilated cardiomyopathy, a NGS workflow based on a panel of 48 cardiomyopathies-causing genes was used to analyze a cohort of 222 DCM patients. Truncating variants were detected on 63 unrelated DCM cases (28.4%). Most of them were identified, as expected, on TTN (29 DCM probands), but truncating variants were also identified on myofibrillar myopathies causing genes in 17 DCM patients (7...
April 24, 2017: Clinical Genetics
https://www.readbyqxmd.com/read/28434749/short-communication-muscle-protein-synthetic-response-to-microparticulated-whey-protein-in-middle-aged-men
#17
C J Mitchell, R F D'Souza, A C Fanning, S D Poppitt, D Cameron-Smith
Whey protein concentrate (WPC) is a high-quality dairy ingredient that is often included in formulated food products designed to stimulate muscle anabolism. Whey protein concentrate can be affected by UHT processing, and its sensory properties are not compatible with some formulated food products. Microparticulated WPC (mWPC) is a novel ingredient that is resistant to heat treatment and has enhanced sensory properties. When 16 healthy middle-aged men consumed 20 g of either WPC or mWPC, both proteins increased plasma essential AA and leucine concentrations with no detectable difference in curve kinetics...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28432118/molecular-basis-for-exercise-induced-fatigue-the-importance-of-strictly-controlled-cellular-ca-2-handling
#18
Arthur J Cheng, Nicolas Place, Håkan Westerblad
The contractile function of skeletal muscle declines during intense or prolonged physical exercise, that is, fatigue develops. Skeletal muscle fibers fatigue acutely during highly intense exercise when they have to rely on anaerobic metabolism. Early stages of fatigue involve impaired myofibrillar function, whereas decreased Ca(2+) release from the sarcoplasmic reticulum (SR) becomes more important in later stages. SR Ca(2+) release can also become reduced with more prolonged, lower intensity exercise, and it is then related to glycogen depletion...
April 21, 2017: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/28408640/long-term-but-not-short-term-high-fat-diet-induces-fiber-composition-changes-and-impaired-contractile-force-in-mouse-fast-twitch-skeletal-muscle
#19
Hiroaki Eshima, Yoshifumi Tamura, Saori Kakehi, Nagomi Kurebayashi, Takashi Murayama, Kyoko Nakamura, Ryo Kakigi, Takao Okada, Takashi Sakurai, Ryuzo Kawamori, Hirotaka Watada
In this study, we investigated the effects of a short-term and long-term high-fat diet (HFD) on morphological and functional features of fast-twitch skeletal muscle. Male C57BL/6J mice were fed a HFD (60% fat) for 4 weeks (4-week HFD) or 12 weeks (12-week HFD). Subsequently, the fast-twitch extensor digitorum longus muscle was isolated, and the composition of muscle fiber type, expression levels of proteins involved in muscle contraction, and force production on electrical stimulation were analyzed. The 12-week HFD, but not the 4-week HFD, resulted in a decreased muscle tetanic force on 100 Hz stimulation compared with control (5...
April 2017: Physiological Reports
https://www.readbyqxmd.com/read/28407937/myofibrillar-protein-gel-properties-are-influenced-by-oxygen-concentration-in-modified-atmosphere-packaged-minced-beef
#20
Hang Wang, Yongkang Luo, Per Ertbjerg
Minced beef was stored for 8days and myofibrillar protein (MP) was extracted to investigate the effect of oxygen concentration (0, 20, 40, 60, and 80%) in modified atmosphere packaging (MAP) on heat-induced gel properties. Compression force of gels was lower when prepared from beef packaged in 0% oxygen, intermediate in 20 to 60% oxygen and greater in 80% oxygen. Total water loss of gels prepared from beef packaged with oxygen (20-80%) was higher and rheology measurements presented higher G' and G″ values...
September 1, 2017: Food Chemistry
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