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Daisuke Ikeda, Hiroki Koyama, Nanami Mizusawa, Nobuhiro Kan-No, Engkong Tan, Shuichi Asakawa, Shugo Watabe
Medaka (Oryzias latipes) is a temperate eurythermal fish that is able to survive over a wide range of water temperatures ranging from near zero to over 30°C throughout the year; it maintains its normal physiological and biochemical processes through temperature acclimation. To determine the mechanisms involved in temperature acclimation of fish, the fast skeletal muscle tissues of medaka underwent global gene expression analysis using next-generation sequencing. Ten individuals were placed into two aquariums at 24°C...
August 2, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Catherine Even, Gilles Abramovici, Florence Delort, Anna F Rigato, Virginie Bailleux, Abel de Sousa Moreira, Patrick Vicart, Felix Rico, Sabrina Batonnet-Pichon, Fatma Briki
Elastic properties of cells are mainly derived from the actin cytoskeleton. However, intermediate filaments are emerging as major contributors to the mechanical properties of cells. Using atomic force microscopy, we studied the elasticity of mouse myoblasts expressing a mutant form of the gene encoding for desmin intermediate filaments, p.D399Y. This variant produces desmin aggregates, the main pathological symptom of myofibrillar myopathies. Here we show that desmin-mutated cells display a 39% increased median elastic modulus compared to wild-type cells...
August 8, 2017: Biophysical Journal
T V Sukhacheva, V A Vaskovsky, A Sh Revishvili, R A Serov
AIM: to analyze the morphological features of the myocardium of the atrial appendages in patients with different forms of atrial fibrillation (AF) and to compare the findings with the clinical parameters of patients. MATERIAL AND METHODS: Light and electron microscopies were used to examine the myocardium of the atrial appendages in adult patients with paroxysmal (PAF), persistent (PrAF), or long-standing persistent atrial fibrillation (LPAF) and in comparison group patients with sinus rhythm without history of AF...
2017: Arkhiv Patologii
Siyu Zhang, Chaoyang Zhang, Yan Qiao, Lujuan Xing, Dacheng Kang, Iftikhar Ali Khan, Ming Huang, Guanghong Zhou
The aim of this study was to investigate the influence of Flavourzyme on protein degradation, antioxidant activity and sensory qualities of Cantonese bacon made at 60°C for 28h. Flavourzyme was used at doses of 8, 16, 24, 32 and 40LAUP/kg of raw meat. Results indicated that Flavourzyme addition in Cantonese bacon promoted proteolysis, which was reflected by the increase of various free amino acids and SDS-PAGE-detected changes in the myogen and myofibrillar proteins. Sensory qualities were improved while TPA (texture profile analysis) was decreased significantly (P<0...
December 15, 2017: Food Chemistry
Magdalena Montowska, Emilia Fornal
This study evaluated the application of label-free, relative quantification of highly processed meat proteins to authenticate processed meat products. Quantitation was based on proteins for which heat-stable and species-specific peptide markers had been detected. Using nano-LC-QTOF-MS/MS, 20 new, heat-stable peptide markers unique to chicken, duck and goose were identified, with significant scores. The method enabled detection of 1%(w/w) of chicken and 1%(w/w) pork in a mixture of the meat of three species, as well as 0...
December 15, 2017: Food Chemistry
Waqas Hanif, Linda Alex, Ya Su, Arti V Shinde, Ilaria Russo, Na Li, Nikolaos G Frangogiannis
Left ventricular dysfunction increases left atrial pressures and causes atrial remodeling. In human subjects, increased left atrial size is a powerful predictor of mortality and adverse events in a broad range of cardiac pathologic conditions. Moreover, structural remodeling of the atrium plays an important role in the pathogenesis of atrial tachyarrhythmias. Despite the potential value of the atrium in assessment of functional endpoints in myocardial disease, atrial pathologic alterations in mouse models of left ventricular disease have not been systematically investigated...
June 21, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Treesa Varghese, Saleena Mathew
Post-mortem biochemical and physiological changes of climbing perch (Anabas testudineus) muscle evaluated during iced storage of 18 days compared with muscle protein content. Changes in the protein fractions, rigor index, pH value, water holding capacity, expressible water content, cook loss, textural parameters, and histochemical properties of the post mortem fish muscle were studied. The whole fish were in full rigor condition till fifth day of storage. Initial muscle pH value of 6.97 was dropped to 6.90 when the fish reached in full rigor condition, this then increased to 7...
July 2017: Journal of Food Science and Technology
Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J Poiesz, Dipak K Dube
In order to better understand the training and athletic activity of horses, we must have complete understanding of the isoform diversity of various myofibrillar protein genes like tropomyosin. Tropomyosin (TPM), a coiled-coil dimeric protein, is a component of thin filament in striated muscles. In mammals, four TPM genes (TPM1, TPM2, TPM3, and TPM4) generate a multitude of TPM isoforms via alternate splicing and/or using different promoters. Unfortunately, our knowledge of TPM isoform diversity in the horse is very limited...
2017: Open veterinary journal
Stefanie Diermeier, Andreas Buttgereit, Sebastian Schürmann, Lilli Winter, Hongyang Xu, Robyn M Murphy, Christoph S Clemen, Rolf Schröder, Oliver Friedrich
The majority of hereditary and acquired myopathies are clinically characterized by progressive muscle weakness. We hypothesized that ongoing derangement of skeletal muscle cytoarchitecture at the single fiber level may precede and be responsible for the progressive muscle weakness. Here, we analyzed the effects of aging in wild-type (wt) and heterozygous (het) and homozygous (hom) R349P desmin knock-in mice. The latter harbor the ortholog of the most frequently encountered human R350P desmin missense mutation...
June 13, 2017: Neurobiology of Aging
Geert H Geesink, Aaron van den Heuvel, Warren Hunt
Meat quality traits of Agile Wallaby (Macropus agilis) M. longissimus (loin) and M. semimembranosus (topside) were investigated. Both muscles exhibited a relatively high pH (>5.7) and dark colour (L*-, a*-, and b*-values). Aging the loins from 2 to 21days p.m. had a significant effect on shear force. However, the results regarding shear force, myofibrillar fragmentation index (MFI) and degradation of desmin and troponin-T suggested that the aging response largely occurred within 2days p.m. Suspension of carcasses from one leg resulted in a side effect on shear force of the loin at 2 and 7days p...
June 29, 2017: Meat Science
T M Ngapo, L Vachon
Particle size analysis has been proposed as a measure of myofibrillar fragmentation resulting from post-mortem proteolysis in meat. The aim of this study was to examine the effect of homogenisation speed, dispersing aggregate size and centrifugation on particle size characteristics of pork loin. Particle size characteristics were significantly (P≤0.023) greater for samples aged 2 than 8d for all but the 80 and 90% quantiles. Differentiation with ageing was only achieved when homogenised at 11,000rpm using the smaller dispersing aggregate (9 vs 13mm rotor diameters)...
July 3, 2017: Meat Science
Cagla Eren Cimenci, Gozde Uzunalli, Ozge Uysal, Fatih Yergoz, Ebru Karaca Umay, Mustafa O Guler, Ayse B Tekinay
Skeletal muscle cells are terminally differentiated and require the activation of muscle progenitor (satellite) cells for their regeneration. There is a clinical need for faster and more efficient treatment methods for acute muscle injuries, and the stimulation of satellite cell proliferation is promising in this context. In this study, we designed and synthesized a laminin-mimetic bioactive peptide (LM/E-PA) system that is capable of accelerating satellite cell activation by emulating the structure and function of laminin, a major protein of the basal membrane of the skeletal muscle...
July 8, 2017: Acta Biomaterialia
Xing Chen, Ronald Keith Tume, Youling Xiong, Xinglian Xu, Guanghong Zhou, Conggui Chen, Tadayuki Nishiumi
The texture, yield and organoleptic properties of comminuted meat products are closely related to the structure and functionality of myofibrillar proteins (MP). To enhance functional properties of MP, high hydrostatic pressure (HHP) has been widely utilized to modify the structure of MP through protein denaturation, solubilization, aggregation or gelation. This modification depends on the protein system (specie, type and formulation) and HHP condition (pressure intensity, pressurizing gradient, duration time, temperature, pressure/temperature and the sequence of application)...
July 5, 2017: Critical Reviews in Food Science and Nutrition
Mangang Wu, Litian Fei, Tao Zhuang, Shumin Lei, Qingfeng Ge, Hai Yu, Jiahao Wang, Yaosong Wang
BACKGROUND: Heat-induced composite gels were prepared with 20g/kg (2%) myofibrillar protein (MP) sol and 100g/kg (10%) olive oil pre-emulsified by MP or non-meat protein in 0.6 M NaCl, at pH 6.2. The effect of different non-meat protein (soy protein isolate, egg-white protein isolate and sodium caseinate) pre-emulsions on the rheological properties and microstructure of MP gel was evaluated. RESULTS: Adding emulsion enhanced the gel strength of MP gel except for the soy protein isolate (SPI) as emulsifier group, but all emulsion group markedly improved (P<0...
July 5, 2017: Journal of the Science of Food and Agriculture
Pauline Le Van Quyen, Philippe Desprez, Angelo Livolsi, Véronique Lindner, Samira Fafi-Kremer, Pauline Helms, Maria Cristina Antal
Introduction  Restrictive cardiomyopathy in fetuses and neonates is extremely rare and has a poor outcome. Its etiology in neonates is elusive: metabolic diseases (e.g., Gaucher, Hurler syndrome), neuromuscular disorders (e.g., muscular dystrophies, myofibrillar myopathies), or rare presentation of genetic syndromes (e.g., Coffin-Lowry syndrome) account for a minority of the cases, the majority remaining idiopathic. Case Study  We report the case of a 17-day-old male infant presenting cardiogenic shock following a restrictive dysfunction of the left ventricle...
April 2017: American Journal of Perinatology Reports
Gianluca Paredi, Roberto Benoni, Giovanni Pighini, Luca Ronda, Adam Dowle, David Ashford, Jerry Thomas, Giovanna Saccani, Roberta Virgili, Andrea Mozzarelli
The production of Parma dry-cured ham involves the steps of salting, drying, and ripening. Although sea salt is the only preserving agent, there are strategies being developed with the goal of reducing salt content in order to decrease its negative impact on consumer health. A 24 h pressure treatment was applied before salting to reduce thickness and inequalities in shape. To evaluate the potential impact of the pressure step on the process outcome, differential proteomic analyses by complementary 2D-PAGE and LC-MS/MS were carried out on exudates collected at day 1, 5, and 18 of the salting phase for hams treated or untreated with pressure...
August 2, 2017: Journal of Agricultural and Food Chemistry
Andrew E Messer, Jasmine Chan, Alex Daley, O'Neal Copeland, Steven B Marston, David J Connolly
Hypertrophic cardiomyopathy (HCM) is the most common single gene inherited cardiomyopathy. In cats (Felix catus) HCM is even more prevalent and affects 16% of the outbred population and up to 26% in pedigree breeds such as Maine Coon and Ragdoll. Homozygous MYBPC3 mutations have been identified in these breeds but the mutations in other cats are unknown. At the clinical and physiological level feline HCM is closely analogous to human HCM but little is known about the primary causative mechanism. Most identified HCM causing mutations are in the genes coding for proteins of the sarcomere...
2017: Frontiers in Physiology
Sarah R Jackman, Oliver C Witard, Andrew Philp, Gareth A Wallis, Keith Baar, Kevin D Tipton
The ingestion of intact protein or essential amino acids (EAA) stimulates mechanistic target of rapamycin complex-1 (mTORC1) signaling and muscle protein synthesis (MPS) following resistance exercise. The purpose of this study was to investigate the response of myofibrillar-MPS to ingestion of branched-chain amino acids (BCAAs) only (i.e., without concurrent ingestion of other EAA, intact protein, or other macronutrients) following resistance exercise in humans. Ten young (20.1 ± 1.3 years), resistance-trained men completed two trials, ingesting either 5...
2017: Frontiers in Physiology
Koichi Himori, Daisuke Tatebayashi, Keita Kanzaki, Masanobu Wada, Håkan Westerblad, Johanna T Lanner, Takashi Yamada
Skeletal muscle weakness is a prominent feature in patients with rheumatoid arthritis (RA). In this study, we investigated whether neuromuscular electrical stimulation (NMES) training protects against skeletal muscle dysfunction in rats with adjuvant-induced arthritis (AIA). AIA was produced by intraarticular injection of complete Freund's adjuvant into the knees of Wistar rats. For NMES training, dorsiflexor muscles were stimulated via a surface electrode (0.5 ms pulse, 50 Hz, 2 s on/4 s off). NMES training was performed every other day for three weeks and consisted of three sets produced at three min intervals...
2017: PloS One
Jing-Yu Wang, Yu-Ling Yang, Xiao-Zhi Tang, Wen-Xi Ni, Lei Zhou
The effects of pulsed ultrasound (PUS) (power: 240w) with varying time (0, 3, 6, 9, 12 and 15min) on rheological and structural properties of chicken myofibrillar protein (CMP) were examined. PUS treatment significantly caused a decrease in the viscosity coefficients (k) but an increase in the flow index (n) value of CMP solutions within short time (0-6min), while had no significant effect for longer time (9-15min). Besides, at 6min, the solubility and microstructure of CMP samples were optimum. The primary structure of CMP was not altered by PUS treatment...
September 2017: Ultrasonics Sonochemistry
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