keyword
MENU ▼
Read by QxMD icon Read
search

Protein digestion

keyword
https://www.readbyqxmd.com/read/29166725/-effect-and-related-mechanism-of-mirna-21-on-hydrogen-peroxide-induced-c-kit-cardiac-stem-cells-apoptosis
#1
R Z Zhao, Y Wang, W W Deng, B Shi, X P Long, Z L Wang, W M Chen
Objective: To explored the effect and related mechanism of miRNA-21 on hydrogen peroxide-induced C-kit(+) cardiac stem cells apoptosis. Methods: C-kit(+) cardiac stem cells were isolated from SD rats by the methods of enzyme digestion and magnetic bead. Cells were divided into the following experimental groups: (1) negative control mimics (NCM)group: cells were transfected with negative control miRNA-21 mimics for 48 hours; (2)mimics group: cells were transfected with miRNA-21 mimics for 48 hours; (3) NCM+ H(2)O(2) group: negative control miRNA-21 mimics were transfected into cells for 48 hours and then treated with 100 μmol H(2)O(2) for 2 hours; (4)mimics+ H(2)O(2) group: miRNA-21 mimics were transfected into cells for 48 hours and then treated with 100 μmol H(2)O(2) for 2 hours...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166671/the-15n-leucine-single-injection-method-allows-for-determining-endogenous-losses-and-true-digestibility-of-amino-acids-in-cecectomized-roosters
#2
Rujiu Hu, Jing Li, Rab Nawaz Soomro, Fei Wang, Yan Feng, Xiaojun Yang, Junhu Yao
This study was conducted to assess the influence of dietary protein content in poultry when using the 15N-leucine single-injection method to determine endogenous amino acid losses (EAALs) in poultry. Forty-eight cecectomized roosters (2.39 ± 0.23 kg) were randomly allocated to eight dietary treatments containing protein levels of 0, 3%, 6%, 9%, 12%, 15%, 18% and 21%. Each bird was precisely fed an experimental diet of 25 g/kg of body weight. After feeding, all roosters were subcutaneously injected with a 15N-leucine solution at a dose of 20 mg/kg of body weight...
2017: PloS One
https://www.readbyqxmd.com/read/29166648/different-response-to-hypoxia-of-adipose-derived-multipotent-cells-from-obese-subjects-with-and-without-metabolic-syndrome
#3
Wilfredo Oliva-Olivera, Isabel Moreno-Indias, Leticia Coín-Aragüez, Said Lhamyani, Juan Alcaide Torres, Sonia Fernández-Veledo, Joan Vendrell, Antonio Camargo, Rajaa El Bekay, Francisco José Tinahones
BACKGROUND/OBJECTIVES: Multiple studies suggest that hypoxia, together with inflammation, could be one of the phenomena involved in the onset and progression of obesity-related insulin resistance. In addition, dysfunction of adipose tissue in obese subjects with metabolic syndrome is associated with decreased angiogenesis. However, some subjects with a high body mass index do not develop metabolic abnormalities associated with obesity. The aim of the current study was to examine the neovascular properties of visceral adipose tissue-derived multipotent mesenchymal cells subjected to hypoxia (hypox-visASCs) from normal-weight subjects (Nw) and obese patients with metabolic syndrome (MS) and without metabolic syndrome (NonMS)...
2017: PloS One
https://www.readbyqxmd.com/read/29166020/solid-digestion-of-demineralized-bone-as-a-method-to-access-potentially-insoluble-proteins-and-post-translational-modifications
#4
Timothy P Cleland
Bone proteomics is an expanding field for understanding protein changes associated with disease as well as characterizing and detecting proteins preserved in fossil bone. Most previous studies have utilized a protocol with demineralization and extraction approach to isolate and characterize proteins from bone. Through near-complete EDTA demineralization, followed by solid digestion of the remaining bone pseudomorph, a total of 92 protein accessions were detected from dog bone. In the EDTA, 14 unique proteins were found, including osteocalcin, an important bone protein...
November 22, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29165646/development-and-application-of-a-recombination-based-library-versus-library-high-throughput-yeast-two-hybrid-rll-y2h-screening-system
#5
Fang Yang, Yingying Lei, Meiling Zhou, Qili Yao, Yichao Han, Xiang Wu, Wanshun Zhong, Chenghang Zhu, Weize Xu, Ran Tao, Xi Chen, Da Lin, Khaista Rahman, Rohit Tyagi, Zeshan Habib, Shaobo Xiao, Dang Wang, Yang Yu, Huanchun Chen, Zhenfang Fu, Gang Cao
Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still required. Here, we developed a recombination-based 'library vs library' Y2H system (RLL-Y2H), by which multi-library screening can be accomplished in a single pool without any individual treatment...
November 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29165370/vitamin-e-bioavailability-mechanisms-of-intestinal-absorption-in-the-spotlight
#6
REVIEW
Emmanuelle Reboul
Vitamin E is an essential fat-soluble micronutrient whose effects on human health can be attributed to both antioxidant and non-antioxidant properties. A growing number of studies aim to promote vitamin E bioavailability in foods. It is thus of major interest to gain deeper insight into the mechanisms of vitamin E absorption, which remain only partly understood. It was long assumed that vitamin E was absorbed by passive diffusion, but recent data has shown that this process is actually far more complex than previously thought...
November 22, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29164873/plasma-protein-turnover-rates-in-rats-using-stable-isotope-labeling-global-proteomics-and-activity-based-protein-profiling
#7
Jordan Ned Smith, Kimberly J Tyrrell, Joshua R Hansen, Dennis George Thomas, Taylor A Murphree, Anil K Shukla, Teresa Luders, James M Madden, YunYing Li, Aaron T Wright, Paul D Piehowski
Protein turnover is important for general health on cellular and organism scales providing a strategy to replace old, damaged, or dysfunctional proteins. Protein turnover also informs of biomarker kinetics, as a better understanding of synthesis and degradation of proteins increases the clinical utility of biomarkers. Here, turnover rates of plasma proteins in rats were measured in vivo using a pulse-chase stable isotope labeling experiment. During the pulse, rats (n=5) were fed 13C6-labeled lysine ("heavy") feed for 23 days to label proteins...
November 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29164733/regulation-of-intestinal-health-by-branched-chain-amino-acids
#8
REVIEW
Hua Zhou, Bing Yu, Jun Gao, John Khun Htoo, Daiwen Chen
Besides its primary role in the digestion and absorption of nutrients, the intestine also interacts with a complex external milieu, and is the first defense line against noxious pathogens and antigens. Dysfunction of the intestinal barrier is associated with enhanced intestinal permeability and development of various gastrointestinal diseases. The branched-chain amino acids (BCAAs) are important nutrients, which are the essential substrates for protein biosynthesis. Recently, emerging evidence showed that BCAAs are involved in maintaining intestinal barrier function...
November 22, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29164337/changes-in-the-midgut-cells-in-the-european-cave-spider-meta-menardi-during-starvation-in-spring-and-autumn
#9
Saška Lipovšek, Gerd Leitinger, Tone Novak, Franc Janžekovič, Szymon Gorgoń, Karolina Kamińska, Magdalena Rost-Roszkowska
During the growth period, in surface habitats, spiders catch enough prey to feed normally. In contrast, in the cave entrance zone, prey may be relatively scarce. Meta menardi inhabits this cave section, resulting in temporary starvation. We studied structural changes in the midgut epithelial cells of M. menardi during a short-term and a medium-term controlled starvation to mimic the occasional starvation in caves, during spring and autumn. Digestive cells, secretory cells and adipocytes were examined before the experimental starvation, in the middle and at the end of starvation...
November 22, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29163616/co-expression-of-arabidopsis-nhx1-and-bar-improves-the-tolerance-to-salinity-oxidative-stress-and-herbicide-in-transgenic-mungbean
#10
Sanjeev Kumar, Angkana Kalita, Richa Srivastava, Lingaraj Sahoo
Mungbean is an important pulse crop extensively cultivated in Southeast Asia for supply of easily digestible protein. Salinity severely limits the growth and productivity of mungbean, and weeding poses nutritional and disease constraints to mungbean cultivation. To pyramid both salt tolerance and protection against herbicide in mungbean, the AtNHX1 encoding tonoplast Na(+)/H(+) antiporter from Arabidopsis, and bar gene associated with herbicide resistance were co-expressed through Agrobacterium-mediated transformation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29163427/antimycobacterial-and-anti-inflammatory-mechanisms-of-baicalin-via-induced-autophagy-in-macrophages-infected-with-mycobacterium-tuberculosis
#11
Qingwen Zhang, Jinxia Sun, Yuli Wang, Weigang He, Lixin Wang, Yuejuan Zheng, Jing Wu, Ying Zhang, Xin Jiang
Tuberculosis (TB) remains a leading killer worldwide among infectious diseases and the effective control of TB is still challenging. Autophagy is an intracellular self-digestion process which has been increasingly recognized as a major host immune defense mechanism against intracellular microorganisms like Mycobacterium tuberculosis (Mtb) and serves as a key negative regulator of inflammation. Clinically, chronic inflammation surrounding Mtb can persist for decades leading to lung injury that can remain even after successful treatment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163019/bile-acid-signaling-pathways-from-the-enterohepatic-circulation-to-the-central-nervous-system
#12
REVIEW
Kim L Mertens, Andries Kalsbeek, Maarten R Soeters, Hannah M Eggink
Bile acids are best known as detergents involved in the digestion of lipids. In addition, new data in the last decade have shown that bile acids also function as gut hormones capable of influencing metabolic processes via receptors such as FXR (farnesoid X receptor) and TGR5 (Takeda G protein-coupled receptor 5). These effects of bile acids are not restricted to the gastrointestinal tract, but can affect different tissues throughout the organism. It is still unclear whether these effects also involve signaling of bile acids to the central nervous system (CNS)...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29162876/transcriptome-analysis-of-the-salivary-glands-of-the-grain-aphid-sitobion-avenae
#13
Yong Zhang, Jia Fan, Jingrui Sun, Frédéric Francis, Julian Chen
Aphid saliva plays important roles in aphid-host interactions, such as assisting aphid digestion, detoxification, activating or suppressing plant defenses. The grain aphid, Sitobion avenae, is one of the most devastating pests of cereals worldwide. In this study, we performed the transcriptome analysis of salivary glands of S. avenae. A total of 33,079 assembled unigenes were identified in the salivary glands of aphids. Of the all obtained unigenes, 15,833(47.86%) and 10,829(32.73%) unigenes showed high similarity to known proteins in Nr and Swiss-Prot databases respectively...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29161498/selective-isolation-of-myosin-subfragment-1-with-a-dna-polyoxovanadate-bioconjugate
#14
Qing Chen, Xue Hu, Dan-Dan Zhang, Xuwei Chen, Jian-Hua Wang
The bioconjugation of a polyoxometalate (POMs), i.e., dodecavanadate (V12O32), to DNA strands produces a functional labeled DNA primer, V12O32-DNA. The grafting of DNA primer onto streptavidin-coated magnetic nanoparticles (SVM) obtains a novel composite, V12O32-DNA@SVM. The high binding-affinity of V12O32 with the ATP binding site in myosin subfragment-1 (S1) facilitates favorable adsorption of myosin, with an efficiency of 99.4% when processing 0.1 mL myosin solution (100 μg/mL) using 0.1 mg composite. Myosin adsorption fits Langmuir model, corresponding to a theoretical adsorption capacity of 613...
November 21, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29161012/top-down-proteomics-ready-for-prime-time
#15
Bifan Chen, Kyle A Brown, Ziqing Lin, Ying Ge
In the post-genomics era, the study of proteins is critical for understanding cellular functions at the molecular level.1-5 Beyond the genetic code, the human proteome is greatly diversified by various genetic variations, alternative splicing of RNA transcripts, and post-translational modifi-cations (PTMs).6,7 In 2013, the term "proteoform" was designated to describe "all of the different molecular forms in which the protein product of a single gene can be found",6 clearing up the confusion in nomenclature and joining research efforts to develop methodologies for pro-teoform characterization...
November 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29161009/a-sensitive-microbead-based-organic-media-assisted-method-for-proteomics-sample-preparation-from-dilute-and-denaturing-solutions
#16
Masato Taoka, Michihiko Fujii, Masahiro Tsuchiya, Takamasa Uekita, Tohru Ichimura
We developed a robust and sensitive sample preparation method for proteomics termed microbead-based and organic-media-assisted proteolysis strategy (BOPs). BOPs combine two advantages of current techniques, (1) unbiased binding of reversed-phase polymeric microbeads to any type of protein and (2) enhanced trypsin digestion efficiency in CH3CN-aqueous solvent systems, into a single-tube workflow. Compared with conventional techniques, this method effectively concentrates proteins and improves proteolytic digestion, and can be used with submicromolar protein samples in dilute and/or denaturing solutions such as 70 % formic acid, 8 M urea, or 7 M guanidine hydrochloride without any sample pretreatment...
November 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29160733/potential-impact-of-inorganic-nanoparticles-on-macronutrient-digestion-titanium-dioxide-nanoparticles-slightly-reduce-lipid-digestion-under-simulated-gastrointestinal-conditions
#17
Qian Li, Ti Li, Chengmei Liu, Glen DeLoid, Georgios Pyrgiotakis, Philip Demokritou, Ruojie Zhang, Hang Xiao, David Julian McClements
Titanium dioxide (TiO2) particles are used in some food products to alter their optical properties, such as whiteness or brightness. These additives typically contain a population of TiO2 nanoparticles (d < 100 nm), which has led to concern about their potential toxicity. The objective of this study was to examine the impact of TiO2 particles on the gastrointestinal fate of oil-in-water emulsions using a simulated gastrointestinal tract (GIT) that includes mouth, stomach, and small intestine phases...
November 21, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/29159365/novel-stool-based-protein-biomarkers-for-improved-colorectal-cancer-screening-a-case-control-study
#18
Linda J W Bosch, Meike de Wit, Thang V Pham, Veerle M H Coupé, Annemieke C Hiemstra, Sander R Piersma, Gideon Oudgenoeg, George L Scheffer, Sandra Mongera, Jochim Terhaar Sive Droste, Frank A Oort, Sietze T van Turenhout, Ilhame Ben Larbi, Joost Louwagie, Wim van Criekinge, Rene W M van der Hulst, Chris J J Mulder, Beatriz Carvalho, Remond J A Fijneman, Connie R Jimenez, Gerrit A Meijer
Background: The fecal immunochemical test (FIT) for detecting hemoglobin is used widely for noninvasive colorectal cancer (CRC) screening, but its sensitivity leaves room for improvement. Objective: To identify novel protein biomarkers in stool that outperform or complement hemoglobin in detecting CRC and advanced adenomas. Design: Case-control study. Setting: Colonoscopy-controlled referral population from several centers...
November 21, 2017: Annals of Internal Medicine
https://www.readbyqxmd.com/read/29159316/in-vitro-starch-hydrolysis-of-chitosan-incorporating-whey-protein-and-wheat-starch-composite-gels
#19
Natasha Yang, John Ashton, Elisabeth Gorczyca, Stefan Kasapis
The study examined the influence of chitosan, incorporated into whey protein and wheat starch thermo gels, on the in-vitro hydrolysis of the polysaccharide. Gels were subjected to the following external conditions containing α-amylase at constant incubation temperature of 37 °C: In the first procedure, they were immersed in phosphate buffer (0.05 M) and maintained at pH 6.9 throughout the entire digestion. In the second instance, they were introduced into a salt solution, with pH and total volume adjusted at times in sync with the human gastrointestinal tract...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29158565/convergent-evolution-of-plant-and-animal-embryo-defences-by-hyperstable-non-digestible-storage-proteins
#20
María Yanina Pasquevich, Marcos Sebastián Dreon, Jian-Wen Qiu, Huawei Mu, Horacio Heras
Plants have evolved sophisticated embryo defences by kinetically-stable non-digestible storage proteins that lower the nutritional value of seeds, a strategy that have not been reported in animals. To further understand antinutritive defences in animals, we analysed PmPV1, massively accumulated in the eggs of the gastropod Pomacea maculata, focusing on how its structure and structural stability features affected its capacity to withstand passage through predator guts. The native protein withstands >50 min boiling and resists the denaturing detergent sodium dodecyl sulphate (SDS), indicating an unusually high structural stability (i...
November 20, 2017: Scientific Reports
keyword
keyword
62983
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"