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https://www.readbyqxmd.com/read/29790070/genotyping-single-sperm-cells-by-universal-marsala-enables-the-acquisition-of-linkage-information-for-combined-pre-implantation-genetic-diagnosis-and-genome-screening
#1
Haitao Wu, Xiaoting Shen, Lei Huang, Yanhong Zeng, Yumei Gao, Lin Shao, Baomin Lu, Yiping Zhong, Benyu Miao, Yanwen Xu, Yali Wang, Yubin Li, Luoxing Xiong, Sijia Lu, X Sunney Xie, Canquan Zhou
PURPOSE: This paper aims to investigate the feasibility of performing pre-implantation genetic diagnosis (PGD) and pre-implantation genetic screening (PGS) simultaneously by a universal strategy without the requirement of genotyping relevant affected family members or lengthy preliminary work on linkage analysis. METHODS: By utilizing a universal Mutated Allele Revealed by Sequencing with Aneuploidy and Linkage Analyses (MARSALA) strategy based on low depth whole genome sequencing (~3x), not involving specific primers' design nor the enrichment of SNP markers for haplotype construction...
May 22, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29781132/egr2-mediates-the-differentiation-of-mouse-uterine-stromal-cells-responsiveness-to-hb-egf-during-decidualization
#2
Liang Yue, Hai-Fan Yu, Zhan-Qing Yang, Xue-Chao Tian, Lian-Wen Zheng, Bin Guo
Although Egr2 is involved in regulating the folliculogenesis and ovulation, there is almost no data describing its physiological function in embryo implantation and decidualization. Here, we showed that Egr2 mRNA was distinctly accumulated in subluminal stromal cells around implanting blastocyst on day 5 of pregnancy as well as in estrogen-activated implantation uterus. Estrogen induced the expression of Egr2 in uterine epithelia. Elevated expression of Egr2 mRNA was also observed in the decidual cells. Silencing of Egr2 by specific siRNA weakened the proliferation of uterine stromal cells and reduced the expression of Ccnd1, Ccnd3, Cdk4, and Cdk6...
May 20, 2018: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/29750165/how-artificial-intelligence-can-improve-our-understanding-of-the-genes-associated-with-endometriosis-natural-language-processing-of-the-pubmed-database
#3
REVIEW
J Bouaziz, R Mashiach, S Cohen, A Kedem, A Baron, M Zajicek, I Feldman, D Seidman, D Soriano
Endometriosis is a disease characterized by the development of endometrial tissue outside the uterus, but its cause remains largely unknown. Numerous genes have been studied and proposed to help explain its pathogenesis. However, the large number of these candidate genes has made functional validation through experimental methodologies nearly impossible. Computational methods could provide a useful alternative for prioritizing those most likely to be susceptibility genes. Using artificial intelligence applied to text mining, this study analyzed the genes involved in the pathogenesis, development, and progression of endometriosis...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29739492/change-in-distribution-of-cytoskeleton-associated-proteins-lasp-1-and-palladin-during-uterine-receptivity-in-the-rat-endometrium
#4
Leigh Nicholson, Laura Lindsay, Christopher R Murphy
The epithelium of the uterine lumen is the first point of contact with the blastocyst before implantation. To facilitate pregnancy, these uterine epithelial cells (UECs) undergo morphological changes specific to the receptive uterus. These changes include basal, lateral and apical alterations in the plasma membrane of UECs. This study looked at the cytoskeletal and focal adhesion-associated proteins, lasp-1 and palladin, in the uterus during early pregnancy in the rat. Two palladin isoforms, 140 kDa and 90 kDa, were analysed, with the migration-associated 140-kDa isoform increasing significantly at the time of implantation when compared with the time of fertilisation...
May 9, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29725444/brachytherapy-for-cervical-cancer-in-septate-uterus-dose-volume-differences-with-tandem-implant-placement-in-right-vs-left-uterine-canal-a-case-report
#5
Naoya Ishibashi, Toshiya Maebayashi, Takuya Aizawa, Masakuni Sakaguchi, Takehiro Nakao, Masahiro Okada
Brachytherapy is a standard treatment modality for locally advanced cervical cancer. In patients with uterine anomalies, the radiation dose to the target volume and the organs at risk can vary depending on the positioning of the brachytherapy tandem implant. However, there have been few reports concerning the use of brachytherapy in patients with uterine anomalies. The present study reports the case of a 55-year-old woman with locally advanced squamous cell carcinoma of the cervix and complete septate uterus...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29720747/wnt-signaling-pathway-in-uterus-of-normal-and-seminal-vesicle-excised-mated-mice-during-pre-implantation-window
#6
Zeinab Latifi, Amir Fattahi, Kobra Hamdi, Aliye Ghasemzadeh, Pouran Karimi, Hamid Reza Nejabati, Marefat Ghaffari Novin, Leila Roshangar, Mohammad Nouri
Introduction: The importance of seminal vesicle secretion and uterine Wnt signaling for uterus preparation and embryo implantation has been described. Materials and Methods: In this study, we evaluated the gene expression of Wnt ligands (Wnt4 and Wnt5a) and their corresponding receptors (Fzd2 and Fzd6) using qRT-PCR and active β-catenin protein levels using western blotting in the uterine tissue of female mice mated with intact and seminal vesicle-excised (SVX) males during the pre-implantation window...
April 2018: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/29709110/making-lineage-decisions-with-biological-noise-lessons-from-the-early-mouse-embryo
#7
REVIEW
Claire S Simon, Anna-Katerina Hadjantonakis, Christian Schröter
Understanding how individual cells make fate decisions that lead to the faithful formation and homeostatic maintenance of tissues is a fundamental goal of contemporary developmental and stem cell biology. Seemingly uniform populations of stem cells and multipotent progenitors display a surprising degree of heterogeneity, primarily originating from the inherent stochastic nature of molecular processes underlying gene expression. Despite this heterogeneity, lineage decisions result in tissues of a defined size and with consistent proportions of differentiated cell types...
April 30, 2018: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29705801/assessment-of-embryo-induced-transcriptomic-changes-in-hamster-uterus-using-rna-seq
#8
Ming-Yu Huang, Wen-Qian Zhang, Miao Zhao, Can Zhu, Jia-Peng He, Ji-Long Liu
BACKGROUND/AIMS: The mouse is widely used as an animal model for studying human embryo implantation. However, the mouse is unique in that both ovarian progesterone and estrogen are critical to implantation, whereas in the majority of species (e.g. human and hamster) implantation can occur in the presence of progesterone alone. METHODS: In this study, we analyzed embryo-induced transcriptomic changes in the hamster uterus during embryo implantation by using RNA-seq...
April 25, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29704394/identification-of-uterine-pacemaker-regions-at-the-myometrial-placental-interface-in-the-rat
#9
E Josiah Lutton, Wim J E P Lammers, Sean James, Hugo A van den Berg, Andrew M Blanks
Coordinated contraction of the uterine smooth muscle is essential to parturition. Histologically and physiologically defined pacemaker structures have not been identified in uterine smooth muscle. Here we report combined electrophysiological and histological evidence of zones associated with pacemaker activity in the rat myometrium (MPPZ = myometrial/placental pacemaking zone) Our method relies crucially on the integration of histological and electrophysiological data in an in silico 3-dimensional reconstruction of the rat myometrium at 10 micrometer resolution We find that MPPZs are closely related with placental sites and the area of disruptive myometrial remodelling surrounding such sites If analogues of the MPPZ are present in the human, defining their histology and physiology will be important steps towards treatment of pre-term birth, pre-eclampsia, and post-partum haemorrhage ABSTRACT: Coordinated uterine contractions are essential for delivering viable offspring in mammals...
April 28, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29692624/comparative-analysis-of-molecular-signatures-suggests-the-use-of-gabapentin-for-the-management-of-endometriosis-associated-pain
#10
Brice Bellessort, Anne Bachelot, Virginie Grouthier, Camille De Lombares, Nicolas Narboux-Neme, Paolo Garagnani, Chiara Pirazzini, Simonetta Astigiano, Luca Mastracci, Anastasia Fontaine, Gladys Alfama, Evelyne Duvernois-Berthet, Giovanni Levi
Background: It has been repetitively shown that the transcription factors DLX5 and DLX6 are drastically downregulated in endometriotic lesions when compared with eutopic endometrium. These findings suggest that regulatory cascades involving DLX5/6 might be at the origin of endometriosis symptoms such as chronic pelvic pain (CPP). We have shown that inactivation of Dlx5 and Dlx5/6 in the mouse uterus results in an endometrial phenotype reminiscent of endometriosis. Methods: We focused on genes that present a similar deregulation in endometriosis and in Dlx5/6 -null mice in search of new endometriosis targets...
2018: Journal of Pain Research
https://www.readbyqxmd.com/read/29688404/heparan-sulfate-proteoglycan-sulfation-regulates-uterine-differentiation-and-signaling-during-embryo-implantation
#11
Yan Yin, Adam Wang, Li Feng, Yu Wang, Hong Zhang, Ivy Zhang, Brent Bany, Liang Ma
To prepare for embryo implantation, uterus must undergo a series of reciprocal interactions between the uterine epithelium and the underlying stroma, which are orchestrated by ovarian hormones. During this process, multiple signaling pathways are activated to direct cell proliferation and differentiation, which render the uterus receptive to the implanting blastocysts. One important modulator of these signaling pathways is the cell surface and extracellular matrix macromolecules, heparan sulfate proteoglycans (HSPGs)...
April 23, 2018: Endocrinology
https://www.readbyqxmd.com/read/29675478/ethical-and-legal-issues-in-gestational-surrogacy
#12
Claudia Casella, Emanuele Capasso, Lucia Terracciano, Paola Delbon, Piergiorgio Fedeli, Francesco Antonio Salzano, Fabio Policino, Massimo Niola
This study originated from events that occurred in 2014 in an Italian hospital, where the embryos of a couple, obtained by means of homologous insemination, were mistakenly implanted into the uterus of another woman who, along with her husband, underwent the same treatment. Faced with this serious adverse circumstance, that gives rise to ethical and legal issues, the authors conducted a comparative examination of how to consider the division of maternity (between biological mother and uterine mother) and the related division of paternity (between genetic father and legal father, husband or partner of the gestational mother)...
2018: Open Medicine (Warsaw, Poland)
https://www.readbyqxmd.com/read/29668899/how-uterine-microbiota-might-be-responsible-for-a-receptive-fertile-endometrium
#13
Marilen Benner, Gerben Ferwerda, Irma Joosten, Renate G van der Molen
BACKGROUND: Fertility depends on a receptive state of the endometrium, influenced by hormonal and anatomical adaptations, as well as the immune system. Local and systemic immunity is greatly influenced by microbiota. Recent discoveries of 16S rRNA in the endometrium and the ability to detect low-biomass microbiota fueled the notion that the uterus may be indeed a non-sterile compartment. To date, the concept of the 'sterile womb' focuses on in utero effects of microbiota on offspring and neonatal immunity...
April 16, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29662714/post-cesarean-scar-endometriosis
#14
Doğan Yıldırım, Cihad Tatar, Ozan Doğan, Adnan Hut, Turgut Dönmez, Muzaffer Akıncı, Mehmet Toptaş, Rahime Nida Bayık
Objective: Endometriosis is seen in women during their reproductive period, where stromal tissue and functional endometrial glands of the uterus are observed outside the uterine cavity. In this study, we aimed to identify the clinical characteristics of our patients who underwent surgery with scar endometriosis and to discuss the surgical results in light of the literature. Materials and Methods: A total of 24 patients who underwent surgery and diagnosed as having endometriosis as the result of a pathologic examination were retrospectively evaluated...
March 2018: Turkish Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29649618/preimplantation-high-resolution-hla-sequencing-using-next-generation-sequencing
#15
Maryam Rafati, Mohammad Mahdi Akhondi, Mohammad Reza Sadeghi, Seyedeh Zahra Tara, Saeed Reza Ghaffari
Hematopoietic Stem Cell Transplantation (HSCT) is the only therapeutic option in a number of heritable hematologic disorders as well as hematologic cancers. Many parents and families fail to find an HLA-identical donor for their affected family member. In such cases, conceiving for a savior baby remains the only option especially in countries without access to national registries. By means of Next Generation Sequencing (NGS) techniques, in a single experiment on single cell products of IVF, a healthy HLA-identical embryo can be implanted in the uterus of a concerned mother...
April 9, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29624291/neonatal-genistein-exposure-and-glucocorticoid-signaling-in-the-adult-mouse-uterus
#16
Shannon D Whirledge, Edwina P Kisanga, Robert H Oakley, John A Cidlowski
BACKGROUND: Female reproductive tract development is sensitive to the endocrine-disrupting potential of environmental estrogens. Early-life exposure to the dietary phytoestrogen genistein impairs fertility and persistently alters the transcriptome in the oviduct and uterus of rodents. Glucocorticoid signaling, which has recently been shown to be essential for normal fertility in the female mouse uterus, is antagonized by genistein. OBJECTIVE: Our goal was to determine whether early-life exposure to genistein disrupts glucocorticoid signaling in the mouse uterus, which may contribute to infertility...
April 5, 2018: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29621956/ovarian-hyperstimulation-reduces-vascular-endothelial-growth-factor-a-during-uterine-receptivity
#17
Kevin Danastas, Camilla M Whittington, Samson N Dowland, Valery Combes, Christopher R Murphy, Laura A Lindsay
The angiogenic factor vascular endothelial growth factor-A (VEGFA) plays a critical role during early pregnancy in many species including the rat, and any alterations in VEGFA levels can severely impact blastocyst implantation rates. The rat ovarian hyperstimulation (OH) model is useful in studying how the induction of superovulation affects VEGFA levels and endometrial receptivity to blastocyst implantation. The present study shows that the major isoform in the rat uterus, Vegf188 , is reduced at the time of receptivity in OH compared to normal pregnancy, whereas there is no change in Vegf164 and Vegf120 messenger RNA (mRNA)...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29614240/expression-of-enpp3-in-human-cyclic-endometrium-a-novel-molecule-involved-in-embryo-implantation
#18
Qianqian Chen, Aijie Xin, Ronggui Qu, Wenbi Zhang, Lu Li, Junling Chen, Xiang Lu, Yongwei Gu, Jing Li, Xiaoxi Sun
Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive. The objective was to determine ENPP3 expression in human endometrium and its roles in endometrial receptivity and embryo implantation. ENPP3 expression was analysed using immunohistochemistry and western blot assay...
April 4, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29609956/arcuate-uterus-is-there-an-impact-on-in-vitro-fertilization-outcomes-after-euploid-embryo-transfer
#19
Eric S Surrey, Mandy Katz-Jaffe, Rebecca L Surrey, Amy Snyder Small, Robert L Gustofson, William B Schoolcraft
OBJECTIVE: To study the impact of the arcuate uterus on euploid blastocyst-stage embryo transfer outcomes after comprehensive chromosomal screening (CCS). DESIGN: Controlled retrospective trial. SETTING: Tertiary care assisted reproduction technology (ART) center. PATIENT(S): Consecutive patients undergoing in vitro fertilization and euploid embryo transfer after CCS during 2014. INTERVENTION(S): Ultrasound examinations and office hysteroscopy; array comparative genomic hybridization to perform CCS after a trophectoderm biopsy...
April 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29605410/environmentally-relevant-levels-of-bisphenol-a-affect-uterine-decidualization-and-embryo-implantation-through-the-estrogen-receptor-serum-and-glucocorticoid-regulated-kinase-1-epithelial-sodium-ion-channel-%C3%AE-subunit-pathway-in-a-mouse-model
#20
Mu Yuan, Minhao Hu, Yiyun Lou, Qijing Wang, Luna Mao, Qitao Zhan, Fan Jin
OBJECTIVE: To investigate whether bisphenol A (BPA) exposure is associated with uterine decidualization and embryo implantation failure in mice. DESIGN: Experimental animal study and in vitro study. SETTING: University-based infertility center. ANIMAL(S): ICR mice. INTERVENTION(S): Mice treated with different doses of BPA; Ishikawa cells cultured in medium of different concentrations of BPA. MAIN OUTCOME MEASURE(S): Embryo implantation sites, uterine weight, quantitative real-time reverse transcriptase-polymerase chain reaction, Western blot analysis, hematoxylin and eosin staining, and immunohistochemical, cell proliferation, and statistical analyses...
April 2018: Fertility and Sterility
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