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https://www.readbyqxmd.com/read/29165317/the-effect-of-electrospun-polycaprolactone-scaffold-morphology-on-human-kidney-epithelial-cells
#1
Todd P Burton, Anthony Corcoran, Anthony Callanan
There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer-based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream. Focus within kidney tissue engineering has been on two-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas...
November 22, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29020662/supercritical-carbon-dioxide-decellularised-pericardium-mechanical-and-structural-characterisation-for-applications-in-cardio-thoracic-surgery
#2
Frank R Halfwerk, Jeroen Rouwkema, Jan A Gossen, Jan G Grandjean
INTRODUCTION: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However, calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterise decellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biological material for uses in cardio-thoracic surgery. METHODS: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensile strength, elastic modulus, fracture toughness and suture retention strength were determined...
October 3, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28952000/the-efficacy-and-sterilisation-of-human-decellularised-dermal-allografts-with-combinations-of-cupric-ions-and-hydrogen-peroxide
#3
S F Leow-Dyke, P Rooney, J N Kearney
Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well known however their combined application is not currently utilised as a decontamination agent in the tissue banking world sector. The aim of this study was to determine the combined concentrations of copper chloride (CuCl2) and H2O2 that have the optimal bactericidal and sporicidal activity on decellularised (dCELL) human dermis...
September 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28916766/stimulatory-secretions-of-airway-epithelial-cells-accelerate-early-repair-of-tracheal-epithelium
#4
Egi Kardia, Rafeezul Mohamed, Badrul Hisham Yahaya
Airway stem/progenitor epithelial cells (AECs) are notable for their differentiation capacities in response to lung injury. Our previous finding highlighted the regenerative capacity of AECs following transplantation in repairing tracheal injury and reducing the severity of alveolar damage associated acute lung injury in a rabbit model. The goal of this study is to further investigate the potential of AECs to re-populate the tracheal epithelium and to study their stimulatory effect on inhibiting pro-inflammatory cytokines, epithelial cell migration and proliferation, and epithelial-to-mesenchymal transition (EMT) process following tracheal injury...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28855577/quantitative-and-organisational-changes-in-mature-extracellular-matrix-revealed-through-high-content-imaging-of-total-protein-fluorescently-stained-in-situ
#5
Gill Holdsworth, Hélène Bon, Marianne Bergin, Omar Qureshi, Ross Paveley, John Atkinson, Linghong Huang, Roohi Tewari, Breda Twomey, Timothy Johnson
Fibrosis is a common driver of end-stage organ failure in most organs. It is characterised by excessive accumulation of extracellular matrix (ECM) proteins. Therapeutic options are limited and novel treatments are urgently required, however current cell-based high-throughput screening (HTS) models to identify molecules affecting ECM accumulation are limited in their relevance or throughput. We report a novel sensitive approach which combines in situ fluorescent staining of accumulated decellularised ECM proteins with automated high-content microscopy...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28815010/development-and-characterisation-of-a-low-concentration-sodium-dodecyl-sulphate-decellularised-porcine-dermis
#6
Jack A Helliwell, Daniel S Thomas, Vaia Papathanasiou, Shervanthi Homer-Vanniasinkam, Amisha Desai, Louise M Jennings, Paul Rooney, John N Kearney, Eileen Ingham
The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment. Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28811169/tissue-engineered-extracellular-matrices-ecms-in-urology-evolution-and-future-directions
#7
REVIEW
N F Davis, E M Cunnane, F J O'Brien, J J Mulvihill, M T Walsh
Autologous gastrointestinal tissue has remained the gold-standard reconstructive biomaterial in urology for >100 years. Mucus-secreting epithelium is associated with lifelong metabolic and neuromechanical complications when implanted into the urinary tract. Therefore, the availability of biocompatible tissue-engineered biomaterials such as extracellular matrix (ECM) scaffolds may provide an attractive alternative for urologists. ECMs are decellularised, biodegradable membranes that have shown promise for repairing defective urinary tract segments in vitro and in vivo by inducing a host-derived tissue remodelling response after implantation...
August 12, 2017: Surgeon: Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland
https://www.readbyqxmd.com/read/28760619/gamma-irradiated-human-amniotic-membrane-decellularised-with-sodium-dodecyl-sulfate-is-a-more-efficient-substrate-for-the-ex-vivo-expansion-of-limbal-stem-cells
#8
G S Figueiredo, S Bojic, P Rooney, S-P Wilshaw, C J Connon, R M Gouveia, C Paterson, G Lepert, H S Mudhar, F C Figueiredo, M Lako
The gold standard substrate for the ex vivo expansion of human limbal stem cells (LSCs) remains the human amniotic membrane (HAM) but this is not a defined substrate and is subject to biological variability and the potential to transmit disease. To better define HAM and mitigate the risk of disease transmission, we sought to determine if decellularisation and/or γ-irradiation have an adverse effect on culture growth and LSC phenotype. Ex vivo limbal explant cultures were set up on fresh HAM, HAM decellularised with 0...
October 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28723473/can-decellularised-prostate-tissue-be-used-to-model-tumour-malignancy
#9
EDITORIAL
Norman J Maitland
This editorial discusses how feasible it will be to predict the invasive capacity of human prostate cancers by measuring their invasion into decellularised extracellular matrix and stresses the multiple factors that affect local metastasis, including immune and stromal cells.
October 2016: European Urology Focus
https://www.readbyqxmd.com/read/28723472/human-prostate-tissue-derived-extracellular-matrix-as-a-model-of-prostate-microenvironment
#10
Walter Cazzaniga, Manuela Nebuloni, Erika Longhi, Irene Locatelli, Raffaele Allevi, Roberta Lucianò, Gelsomina Senatore, Eugenio Ventimiglia, Vito Cucchiara, Luca Genovese, Francesco Montorsi, Massimo Alfano, Andrea Salonia, Ilaria Cavarretta
BACKGROUND: Clinical experience highlights the wide heterogeneity of primary prostate cancer (PPCa), even when potentially related to the same grade and stage. Currently available prediction tools and biomarkers do not always allow for early recognition of PPCa aggressive phenotype, sometimes making it impossible to distinguish among men harbouring indolent tumours or life-threatening disease. OBJECTIVE: To establish a novel ex vivo/in vitro model suitable to estimate the invasive phenotype of PPCa cells (PPCaC)...
October 2016: European Urology Focus
https://www.readbyqxmd.com/read/28647518/increasing-the-accuracy-of-proteomic-typing-by-decellularisation-of-amyloid-tissue-biopsies
#11
P Patrizia Mangione, Giuseppe Mazza, Janet A Gilbertson, Nigel B Rendell, Diana Canetti, Sofia Giorgetti, Luca Frenguelli, Marco Curti, Tamer Rezk, Sara Raimondi, Mark B Pepys, Philip N Hawkins, Julian D Gillmore, Graham W Taylor, Massimo Pinzani, Vittorio Bellotti
Diagnosis and treatment of systemic amyloidosis depend on accurate identification of the specific amyloid fibril protein forming the tissue deposits. Confirmation of monoclonal immunoglobulin light chain amyloidosis (AL), requiring cytotoxic chemotherapy, and avoidance of such treatment in non-AL amyloidosis, are particularly important. Proteomic analysis characterises amyloid proteins directly. It complements immunohistochemical staining of amyloid to identify fibril proteins and gene sequencing to identify mutations in the fibril precursors...
August 8, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28599006/long-term-cryopreservation-of-decellularised-oesophagi-for-tissue-engineering-clinical-application
#12
Luca Urbani, Panagiotis Maghsoudlou, Anna Milan, Maria Menikou, Charlotte Klara Hagen, Giorgia Totonelli, Carlotta Camilli, Simon Eaton, Alan Burns, Alessandro Olivo, Paolo De Coppi
Oesophageal tissue engineering is a therapeutic alternative when oesophageal replacement is required. Decellularised scaffolds are ideal as they are derived from tissue-specific extracellular matrix and are non-immunogenic. However, appropriate preservation may significantly affect scaffold behaviour. Here we aim to prove that an effective method for short- and long-term preservation can be applied to tissue engineered products allowing their translation to clinical application. Rabbit oesophagi were decellularised using the detergent-enzymatic treatment (DET), a combination of deionised water, sodium deoxycholate and DNase-I...
2017: PloS One
https://www.readbyqxmd.com/read/28449861/the-effects-of-irradiation-dose-and-storage-time-following-treatment-on-the-viscoelastic-properties-of-decellularised-porcine-super-flexor-tendon
#13
Anthony Herbert, Jennifer H Edwards, Gemma L Jones, Eileen Ingham, John Fisher
Decellularised porcine super flexor tendon (pSFT) offers a promising solution to the replacement of damaged anterior cruciate ligament. It is desirable to package and terminally sterilise the acellular grafts to eliminate any possible harmful pathogens. However, irradiation techniques can damage the collagen structure and consequently reduce the mechanical properties. The aims of this study were to investigate the effects of irradiation sterilisation of varying dosages on the viscoelastic properties of the decellularised pSFT...
May 24, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28441344/collagenous-extracellular-matrix-biomaterials-for-tissue-engineering-lessons-from-the-common-sea-urchin-tissue
#14
REVIEW
Kheng Lim Goh, David F Holmes
Scaffolds for tissue engineering application may be made from a collagenous extracellular matrix (ECM) of connective tissues because the ECM can mimic the functions of the target tissue. The primary sources of collagenous ECM material are calf skin and bone. However, these sources are associated with the risk of having bovine spongiform encephalopathy or transmissible spongiform encephalopathy. Alternative sources for collagenous ECM materials may be derived from livestock, e.g., pigs, and from marine animals, e...
April 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28360084/the-revitalisation-of-flexor-tendon-allografts-with-bone-marrow-stromal-cells-and-mechanical-stimulation-an-ex-vivo-model-revitalising-flexor-tendon-allografts
#15
J H Wu, A R Thoreson, A Gingery, K N An, S L Moran, P C Amadio, C Zhao
OBJECTIVES: The present study describes a novel technique for revitalising allogenic intrasynovial tendons by combining cell-based therapy and mechanical stimulation in an ex vivo canine model. METHODS: Specifically, canine flexor digitorum profundus tendons were used for this study and were divided into the following groups: (1) untreated, unprocessed normal tendon; (2) decellularised tendon; (3) bone marrow stromal cell (BMSC)-seeded tendon; and (4) BMSC-seeded and cyclically stretched tendon...
March 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28303723/decellularized-material-as-scaffolds-for-tissue-engineering-studies-in-long-gap-esophageal-atresia
#16
REVIEW
Esmond Lee, Anna Milan, Luca Urbani, Paolo De Coppi, Mark W Lowdell
Esophageal atresia refers to an anomaly in foetal development in which the esophagus terminates in a blind end. Whilst surgical correction is achievable in most patients, when a long gap is present it still represents a major challenge associated with higher morbidity and mortality. In this context, tissue engineering could represent a successful alternative to restore oesophageal function and structure. Naturally derived biomaterials made of decellularized tissues retain native extracellular matrix architecture and composition, providing a suitable bed for the anchorage and growth of relevant cell types...
May 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28259835/scaffolds-for-3d-in-vitro-culture-of-neural-lineage-cells
#17
REVIEW
Ashley R Murphy, Andrew Laslett, Carmel M O'Brien, Neil R Cameron
Understanding how neurodegenerative disorders develop is not only a key challenge for researchers but also for the wider society, given the rapidly aging populations in developed countries. Advances in this field require new tools with which to recreate neural tissue in vitro and produce realistic disease models. This in turn requires robust and reliable systems for performing 3D in vitro culture of neural lineage cells. This review provides a state of the art update on three-dimensional culture systems for in vitro development of neural tissue, employing a wide range of scaffold types including hydrogels, solid porous polymers, fibrous materials and decellularised tissues as well as microfluidic devices and lab-on-a-chip systems...
March 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28240419/enhanced-antimicrobial-effects-of-decellularized-extracellular-matrix-cormatrix-with-added-vancomycin-and-gentamicin-for-device-implant-protection
#18
Thomas F Deering, Carlos Chang, Carl Snyder, Selvamuthu K Natarajan, Robert Matheny
BACKGROUND: The incidence of cardiac implantable electronic device (CIED) infections has risen significantly over the past years. Although several devices are currently available to decrease the incidence of infection, most are made from nonviable synthetic material and are more prone to infection than vascularized tissue. OBJECTIVE: This study was undertaken to assess the resistance to infection of the CorMatrix CanGaroo (CorMatrix Cardiovascular, Roswell, GA, USA), a CIED envelope made of decellularized extracellular matrix (ECM) hydrated in different antibiotic solutions...
June 2017: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/28197989/growth-plate-extracellular-matrix-derived-scaffolds-for-large-bone-defect-healing
#19
G M Cunniffe, P J Díaz-Payno, J S Ramey, O R Mahon, A Dunne, E M Thompson, F J O'Brien, D J Kelly
Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix cues which contribute to long bone growth, suggesting that biomaterials derived from its extracellular matrix (ECM) may be uniquely suited to promoting bone regeneration. The goal of this study was to generate porous scaffolds from decellularised GP ECM and to evaluate their ability to enhance host mediated bone regeneration following their implantation into critically-sized rat cranial defects...
February 14, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28112872/development-of-decellularized-conjunctiva-as-a-substrate-for-the-ex-vivo-expansion-of-conjunctival-epithelium
#20
Shivani Kasbekar, Stephen B Kaye, Rachel L Williams, Rosalind M K Stewart, Sophie Leow-Dyke, Paul Rooney
This study was performed to develop a method to decellularize human conjunctiva and to characterize the tissue in terms of its deoxyribose nucleic acid (DNA) content, tensile strength, collagen denaturation, basement membrane, extracellular matrix components and its potential to support conjunctival epithelial growth. Human conjunctival tissues were subjected to a decellularization process involving hypotonic detergent and nuclease buffers. Variations in sodium dodecyl sulfate concentration (0.05-0.5%, w/v) were tested to determine the appropriate concentration of detergent buffer...
January 23, 2017: Journal of Tissue Engineering and Regenerative Medicine
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