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https://www.readbyqxmd.com/read/28197989/growth-plate-extracellular-matrix-derived-scaffolds-for-large-bone-defect-healing
#1
G M Cunniffe, P J Díaz-Payno, J S Ramey, O R Mahon, A Dunne, E M Thompson, F J O'Brien, D J Kelly
Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix cues which contribute to long bone growth, suggesting that biomaterials derived from its extracellular matrix (ECM) may be uniquely suited to promoting bone regeneration. The goal of this study was to generate porous scaffolds from decellularised GP ECM and to evaluate their ability to enhance host mediated bone regeneration following their implantation into critically-sized rat cranial defects...
February 14, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28112872/development-of-decellularised-conjunctiva-as-a-substrate-for-the-ex-vivo-expansion-of-conjunctival-epithelium
#2
Shivani Kasbekar, Stephen B Kaye, Rachel L Williams, Rosalind Mk Stewart, Sophie Leow-Dyke, Paul Rooney
PURPOSE: To develop a method to decellularise human conjunctiva. To characterise the tissue in terms of its DNA content, tensile strength, collagen denaturation, basement membrane, extracellular matrix components and its potential to support conjunctival epithelial growth. METHODS: Human conjunctival tissues were subjected to a decellularisation process involving hypotonic detergent and nuclease buffers. Variations in sodium dodecyl sulphate (SDS) concentration (0...
January 23, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27995582/denudation-of-human-amniotic-membrane-by-a-novel-process-and-its-characterisations-for-biomedical-applications
#3
R Sripriya, R Kumar
This study was aimed to investigate the suitability of a modified method to get decellularised human amniotic membrane (DHAM). The obtained membrane was subjected to physico-chemical and biological evaluations to validate its potential for biomedical applications. The human amniotic membrane was processed with detergent and alkali followed by enzymatic treatments. Hematoxylin and eosin (H&E) and Masson's trichrome staining of membrane were in accordance with conjectures: the decellularised membrane stained for extracellular matrix is rich in collagen...
December 2016: Progress in biomaterials
https://www.readbyqxmd.com/read/27967204/mmp-1-activation-contributes-to-airway-smooth-muscle-growth-and-asthma-severity
#4
Shams-Un-Nisa Naveed, Debbie Clements, David J Jackson, Christopher Philp, Charlotte K Billington, Irshad Soomro, Catherine Reynolds, Timothy W Harrison, Sebastian L Johnston, Dominick E Shaw, Simon R Johnson
INTRODUCTION: Matrix metalloproteinase-1 and mast cells are present in the airways of people with asthma. We hypothesised that matrix metalloproteinase-1 could be activated by mast cells and increase asthma severity. METHODS: Patients with stable asthma and healthy controls underwent spirometry, methacholine challenge, bronchoscopy and their airway smooth muscle cells were grown in culture. A second asthma group and controls had symptom scores, spirometry and bronchoalveolar lavage before and after rhinovirus-induced asthma exacerbations...
December 14, 2016: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/27943656/decellularisation-of-human-donor-aortic-and-pulmonary-valved-conduits-using-low-concentration-sds
#5
Tayyebeh Vafaee, Daniel Thomas, Amisha Desai, Louise M Jennings, Helen Berry, Paul Rooney, John Kearney, John Fisher, Eileen Ingham
The clinical use of decellularised cardiac valve allografts is increasing. Long term data will be required to determine whether they outperform conventional cryopreserved allografts. Valves decellularised using different processes may show varied long-term outcomes. It is therefore important to understand the effects of specific decellularisation technologies on the characteristics of donor heart valves. Human cryopreserved aortic and pulmonary valved conduits were decellularised using hypotonic buffer, 0.1% (w/v) SDS and nuclease digestion...
December 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27922061/thermo-sensitive-hydrogels-combined-with-decellularised-matrix-deliver-bfgf-for-the-functional-recovery-of-rats-after-a-spinal-cord-injury
#6
He-Lin Xu, Fu-Rong Tian, Cui-Tao Lu, Jie Xu, Zi-Liang Fan, Jing-Jing Yang, Pian-Pian Chen, Ya-Dong Huang, Jian Xiao, Ying-Zheng Zhao
Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27846786/systematic-comparison-of-protocols-for-the-preparation-of-human-articular-cartilage-for-use-as-scaffold-material-in-cartilage-tissue-engineering
#7
Cornelia Schneider, Johannes Lehmann, Gerjo J V M van Osch, Florian Hildner, Andreas Teuschl, Xavier Monforte, David Miosga, Patrick Heimel, Eleni Priglinger, Heinz Redl, Suanne Wolbank, Sylvia Nürnberger
Natural extracellular matrix-derived biomaterials from decellularized allogenic tissues are of increasing interest for tissue engineering because their structure and composition provide a complexity that is not achievable with current manufacturing techniques. The prerequisite to bring allogenic tissue from bench to bedside as a functional biomaterial is the full removal of cells while preserving most of its native characteristics such as structure and composition. The exceptionally dense structure of articular cartilage, however, poses a special challenge for decellularization, scaffold preparation, and reseeding...
December 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27806678/decellularization-and-characterization-of-porcine-superflexor-tendon-a-potential-anterior-cruciate-ligament-replacement
#8
Gemma Jones, Anthony Herbert, Helen Berry, Jennifer Helen Edwards, John Fisher, Eileen Ingham
The porcine superflexor tendon (SFT) was identified as having appropriate structure and properties for development of a decellularized device for use in anterior cruciate ligament reconstruction. SFTs were decellularized using a combination of freeze-thaw and washes in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus proteinase inhibitors, followed by nuclease treatment and sterilization using peracetic acid. The decellularized biological scaffold was devoid of cells and cell remnants and contained only 13 ng/mg (dry weight) residual total DNA...
February 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27767181/efficient-recellularisation-of-decellularised-whole-liver-grafts-using-biliary-tree-and-foetal-hepatocytes
#9
Satoshi Ogiso, Kentaro Yasuchika, Ken Fukumitsu, Takamichi Ishii, Hidenobu Kojima, Yuya Miyauchi, Ryoya Yamaoka, Junji Komori, Hokahiro Katayama, Takayuki Kawai, Elena Yukie Yoshitoshi, Sadahiko Kita, Katsutaro Yasuda, Shinji Uemoto
A whole-organ regeneration approach, using a decellularised xenogeneic liver as a scaffold for the construction of a transplantable liver was recently reported. Deriving suitable scaffolds was the first step towards clinical application; however, effective recellularisation remains to be achieved. This report presents a strategy for the improvement of the recellularisation process, using novel cell-seeding technique and cell source. We evaluated recellularised liver grafts repopulated through the portal vein or the biliary duct with mice adult hepatocytes or E14...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27725772/mechanisms-underlying-heterologous-skin-scaffold-mediated-tissue-remodeling
#10
Kallyne K O Mimura, Andréia R Moraes, Aline C Miranda, Rebecca Greco, Tahera Ansari, Paul Sibbons, Karin V Greco, Sonia M Oliani
Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14...
October 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27701132/automated-bioreactor-system-for-cartilage-tissue-engineering-of-human-primary-nasal-septal-chondrocytes
#11
Sascha Princz, Ulla Wenzel, Hanna Tritschler, Silke Schwarz, Christian Dettmann, Nicole Rotter, Martin Hessling
An automated bioreactor system for three-dimensional (3D) cultivation of facial cartilage replacement matrices (e.g. whole human auricles) with automatised medium exchange, gas flow and temperature control was developed. The measurement of O2 saturation and pH value in the medium was performed with a non-invasive optical method. The whole system can be observed via remote monitoring worldwide. First results demonstrated that the complete system remained sterile throughout a period of 42 days. Human chondrocytes migrated into the employed cartilage replacement matrix consisting of decellularised porcine nasoseptal cartilage (pNSC)...
October 4, 2016: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/27679415/proliferation-and-apoptosis-processes-in-the-seasonal-testicular-development-of-the-wild-daurian-ground-squirrel-citellus-dauricus-brandt-1844
#12
Yingying Han, Jinqi Zhan, Ying Xu, Fengwei Zhang, Zhengrong Yuan, Qiang Weng
The aim of the present study was to elucidate the regulatory role of cell proliferation and apoptosis in testicular development of wild Daurian ground squirrels during the breeding season (April), the non-breeding season (June) and before hibernation (September). Gross mass and hormonal analysis showed that the testis:body mass ratio and plasma testosterone concentration fluctuated seasonally, with a peak in April and lowest values in June. Similarly, spermatogenesis was fully developed in April but suppressed in June and September...
September 28, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27636889/extracellular-matrix-derived-hydrogels-for-dental-stem-cell-delivery
#13
Aiswarya Viswanath, Julie Vanacker, Loïc Germain, Julian G Leprince, Anibal Diogenes, Kevin M Shakesheff, Lisa J White, Anne des Rieux
Decellularized mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration...
January 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27619480/retrospective-observational-analysis-of-the-use-of-an-architecturally-unique-dermal-regeneration-template-derma-pure%C3%A2-for-the-treatment-of-hard-to-heal-wounds
#14
Howard Kimmel, Haley Gittleman
The purpose of this analysis was to evaluate the use of DermaPure, a decellularised human skin allograft, in the treatment of a variety of challenging wounds. This retrospective observational analysis reviewed a total of 37 patients from 29 different wound clinics across the USA. Each patient received one application of DermaPure which was followed until complete closure. A statistical analysis was performed with the end point being complete healing. All wounds on average, had a duration of 56 weeks and healed in an average time of 10·58 weeks...
September 13, 2016: International Wound Journal
https://www.readbyqxmd.com/read/27589945/comparative-proteomic-assessment-of-matrisome-enrichment-methodologies
#15
Lukas Krasny, Angela Paul, Patty Wai, Beatrice A Howard, Rachael C Natrajan, Paul H Huang
The matrisome is a complex and heterogeneous collection of extracellular matrix (ECM) and ECM-associated proteins that play important roles in tissue development and homeostasis. While several strategies for matrisome enrichment have been developed, it is currently unknown how the performance of these different methodologies compares in the proteomic identification of matrisome components across multiple tissue types. In this study, we perform a comparative proteomic assessment of two widely used decellularisation protocols and two extraction methods to characterise the matrisome in four murine organs (heart, mammary gland, lung and liver)...
September 2, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27386840/a-new-strategy-for-the-decellularisation-of-large-equine-tendons-as-biocompatible-tendon-substitutes
#16
M Bottagisio, A F Pellegata, F Boschetti, M Ferroni, M Moretti, A B Lovati
Tendon ruptures and/or large losses remain to be a great clinical challenge and often require full replacement of the damaged tissue. The use of auto- and allografts or engineered scaffolds is an established approach to restore severe tendon injuries. However, these grafts are commonly related to scarce biocompatibility, site morbidity, chronic inflammation and poor biomechanical properties. Recently, the decellularisation techniques of allo- or xenografts using specific detergents have been studied and have been found to generate biocompatible substitutes that resemble the native tissue...
2016: European Cells & Materials
https://www.readbyqxmd.com/read/27173864/percutaneous-pulmonary-valve-replacement-using-completely-tissue-engineered-off-the-shelf-heart-valves-six-month-in-vivo-functionality-and-matrix-remodelling-in-sheep
#17
Boris Schmitt, Hendrik Spriestersbach, Darach O H-Icí, Torben Radtke, Marco Bartosch, Heiner Peters, Matthias Sigler, Laura Frese, Petra E Dijkman, Frank P T Baaijens, Simon P Hoerstrup, Felix Berger
AIMS: The objective was to implant a stented decellularised tissue-engineered heart valve (sdTEHV) percutaneously in an animal model, to assess its in vivo functionality and to examine the repopulation and remodelling of the valvular matrix by the recipient's autologous cells. METHODS AND RESULTS: Prototypes of sdTEHV were cultured in vitro, decellularised and percutaneously implanted into the pulmonary position in 15 sheep. Functionality was assessed monthly by intracardiac echocardiography (ICE)...
May 17, 2016: EuroIntervention
https://www.readbyqxmd.com/read/27144773/on-the-automatic-decellularisation-of-porcine-aortae-a-repeatability-study-using-a-non-enzymatic-approach
#18
Rory O'Connor Mooney, Niall Francis Davis, David Hoey, Lisa Hogan, Timothy M McGloughlin, Michael T Walsh
OBJECTIVE: To investigate the repeatability of automatic decellularisation of porcine aortae using a non-enzymatic approach, addressing current limitations associated with other automatic decellularisation processes. MATERIALS AND METHODS: Individual porcine aortae (n = 3) were resected and every third segment (n = 4) was allocated to one of three different groups: a control or a manually or automatically decellularised group. Manual and automatic decellularisation was performed using Triton X-100 (2% v/v) and sodium deoxycholate...
2016: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/27063250/bi-linear-mechanical-property-determination-of-acellular-human-patellar-tendon-grafts-for-use-in-anterior-cruciate-ligament-replacement
#19
Anthony Herbert, Christopher Brown, Paul Rooney, John Kearney, Eileen Ingham, John Fisher
Anterior cruciate ligament rupture is rising in its prevalence amongst the young and those with physically active lifestyles. Acellular human patellar tendon (PT) grafts offer a promising restoration solution, returning knee joint stability and overcoming some of the current disadvantages of autologous or allogeneic grafts. However, it is necessary to ensure that the decellularisation bio-processes involved do not cause structural changes in the microstructure of the tendon tissue that may adversely affect the mechanical properties, particularly with respect to the physiological range of loading...
June 14, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/27062181/optimization-of-sds-exposure-on-preservation-of-ecm-characteristics-in-whole-organ-decellularization-of-rat-kidneys
#20
M He, A Callanan, K Lagaras, J A M Steele, M M Stevens
Renal transplantation is well established as the optimal form of renal replacement therapy but is restricted by the limited pool of organs available for transplantation. The whole organ decellularisation approach is leading the way for a regenerative medicine solution towards bioengineered organ replacements. However, systematic preoptimization of both decellularization and recellularization parameters is essential prior to any potential clinical application and should be the next stage in the evolution of whole organ decellularization as a potential strategy for bioengineered organ replacements...
April 8, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
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