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https://www.readbyqxmd.com/read/27925010/the-effects-of-a-simple-method-for-cryopreservation-and-thawing-procedures-on-cord-blood-derived-dc-based-esophageal-carcinoma-vaccine
#1
J Yu, L Xie, S Chen, J Zhang, G Guo, B Chen
BACKGROUND: Producing sufficient numbers of DCs at one time point and subsequently cryopreserving the generated DCs in ready-for-use aliquots for clinical application is useful in cancer treatment. OBJECTIVE: To study the effects of a simplified cryopreservation method and thawing procedures acting on the biological characteristics and specific cytotoxic activity of cord blood derived DC-based esophageal carcinoma vaccine. MATERIALS AND METHODS: CD34+ hematopoietic stem cells were isolated from cord blood using CD34+ Progenitor Cell Isolation Kit by magnetic cell sorting system (MACS)...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27905054/crosstalk-between-shh-and-stemness-state-signaling-pathways-in-esophageal-squamous-cell-carcinoma
#2
Maryam Najafi, Mohammad Reza Abbaszadegan, Abolfazl Rad, Mahtab Dastpak, Samaneh Boroumand-Noughabi, Mohammad Mahdi Forghanifard
The expression of GLI1 as a downstream gene of sonic hedgehog (Hh) pathway, studied in a variety of cancers including esophageal squamous cell carcinoma (ESCC). However, the interaction of Hh with other developmental pathways needs to be elucidated. In this study, we aimed to investigate the correlation of GLI1 expression with transcription factors (TFs) of stem cell signaling pathways, and their association with clinico-pathological data of ESCC. Using real-time PCR, we assessed the expression of GLI1 mRNA in 49 ESCC patients, and analyzed the correlation between GLI1 and selected TFs...
November 30, 2016: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/27872710/linc-ror-and-its-spliced-variants-2-and-4-are-significantly-up-regulated-in-esophageal-squamous-cell-carcinoma
#3
Reza Sahebi, Mahshid Malakootian, Baharak Balalaee, Alireza Shahryari, Masoud Khoshnia, Mohammad Reza Abbaszadegan, Abdolvahab Moradi, Seyed Javad Mowla
OBJECTIVES: Similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. Reprogramming-related transcription factors, also known as Yamanaka factors (OCT4, SOX2, KLF4, and c-MYC), are also well-known oncogenes promoting cancer initiation, progression, and cellular transformation into cancer stem cells. Long non-coding RNAs (lncRNAs) are a major class of RNA molecules with emerging roles in stem cell pluripotency, cellular reprogramming, cellular transformation, and tumorigenesis...
October 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27826618/valproic-acid-inhibits-irradiation-induced-epithelial-mesenchymal-transition-and-stem-cell-like-characteristics-in-esophageal-squamous-cell-carcinoma
#4
Ayako Kanamoto, Itasu Ninomiya, Shinichi Harada, Tomoya Tsukada, Koichi Okamoto, Shinichi Nakanuma, Seisho Sakai, Isamu Makino, Jun Kinoshita, Hironori Hayashi, Katsunobu Oyama, Tomoharu Miyashita, Hidehiro Tajima, Hiroyuki Takamura, Sachio Fushida, Tetsuo Ohta
Esophageal carcinoma is one of the most aggressive malignancies, and is characterized by poor response to current therapy and a dismal survival rate. In this study we investigated whether irradiation induces epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC) TE9 cells and whether the classic histone deacetylase (HDAC) inhibitor valproic acid (VPA) suppresses these changes. First, we showed that 2 Gy irradiation induced spindle cell-like morphologic changes, decreased expression of membranous E-cadherin, upregulated vimentin expression, and altered the localization of β-catenin from its usual membrane-bound location to cytoplasm in TE9 cells...
November 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27822417/tumor-endothelial-cells-promote-metastasis-and-cancer-stem-cell-like-phenotype-through-elevated-epiregulin-in-esophageal-cancer
#5
Lichao Sun, Jian Pan, Long Yu, Huiqi Liu, Xiong Shu, Lixin Sun, Jinning Lou, Zhihua Yang, Yuliang Ran
Tumor endothelial cells have been found to be associated with metastasis and cancer progression. In this study, we reported that human esophageal cancer endothelial cells (HECEC), unlike corresponding human esophageal normal endothelial cells (HENEC) displayed several distinct feature couple with unique gene expression profile. Further studies showed that HECEC can enhance migration, invasion and self-renewal properties of esophageal carcinoma cell in vitro by a direct cell-cell interaction. In vivo assay demonstrated that HECEC could significantly enhance the invasion and lung metastasis of esophageal cancer cells...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27807078/goblet-cell-ratio-in-combination-with-differentiation-and-stem-cell-markers-in-barrett-esophagus-allow-distinction-of-patients-with-and-without-esophageal-adenocarcinoma
#6
Raphael Schellnegger, Anne Quante, Susanne Rospleszcz, Martina Schernhammer, Bettina Höhl, Moritz Tobiasch, Agnieszka Pastula, Anna Brandtner, Julian A Abrams, Konstantin Strauch, Roland M Schmid, Michael Vieth, Timothy C Wang, Michael Quante
The increasing incidence of esophageal adenocarcinoma (EAC) is mirrored by the increasing prevalence of Barrett esophagus, a precursor lesion resulting in a large number of individuals "at risk" for this lethal malignancy. Among patients with Barrett esophagus, only about 0.3% annually will develop EAC. Because large numbers of patients are followed in endoscopic surveillance, there is a need for risk prediction among a growing population of patients with Barrett esophagus. We identified four potential biomarkers from an inflammation (IL1β)-dependent mouse model of Barrett esophagus and tested them in 189 patients with Barrett esophagus with and without high-grade dysplasia (HGD)/early cancer (T1)...
November 2, 2016: Cancer Prevention Research
https://www.readbyqxmd.com/read/27748881/expression-status-of-cd44-and-cd133-as-a-prognostic-marker-in-esophageal-squamous-cell-carcinoma-treated-with-neoadjuvant-chemotherapy-followed-by-radical-esophagectomy
#7
Koichi Okamoto, Itasu Ninomiya, Yoshinao Ohbatake, Atsushi Hirose, Tomoya Tsukada, Shinichi Nakanuma, Seisho Sakai, Jun Kinoshita, Isamu Makino, Keishi Nakamura, Hironori Hayashi, Katsunobu Oyama, Masafumi Inokuchi, Hisatoshi Nakagawara, Tomoharu Miyashita, Tajima Hidehiro, Hiroyuki Takamura, Sachio Fushida, Tetsuo Ohta
Cancer stem cells (CSCs) have self-renewal and pluripotency capabilities and contribute to cancer progression and chemoresistance. It has been proposed that the treatment resistance and heterogeneity of CSCs are deeply involved in the prognosis of patients with esophageal squamous cell carcinoma (ESCC). The objective of this study was to identify the influence of the expression status of the CSC markers CD44 and CD133 on chemotherapeutic efficacy and prognosis in ESCC patients who underwent radical esophagectomy after neoadjuvant chemotherapy (NAC)...
September 28, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27742014/kdm4c-a-h3k9me3-histone-demethylase-is-involved-in-the-maintenance-of-human-escc-initiating-cells-by-epigenetically-enhancing-sox2-expression
#8
Xiang Yuan, Jinyu Kong, Zhikun Ma, Na Li, Ruinuo Jia, Yiwen Liu, Fuyou Zhou, Qimin Zhan, Gang Liu, Shegan Gao
Our studies investigating the existence of tumor-initiating cell (TIC) populations in human esophageal squamous cell carcinoma (ESCC) had identified a subpopulation of cells isolated from ESCC patient-derived tumor specimens marked by an ALDH(bri+) phenotype bear stem cell-like features. Importantly, KDM4C, a histone demethylase was enhanced in ALDH(bri+) subpopulation, suggesting that strategies interfering with KDM4C may be able to target these putative TICs. In the present study, by genetic and chemical means, we demonstrated that, KDM4C blockade selectively decreased the ESCC ALDH(bri+) TICs population in vitro and specifically targeted the TICs in ALDH(bri+)-derived xenograft, retarding engraftment...
October 2016: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/27708180/mid-esophagus-columnar-metaplasia-what-is-the-biopathogenic-pathway
#9
Inês Rolim, Rita Vale Rodrigues, António Bettencourt, Rita Barros, Vânia Camilo, António Dias Pereira, Raquel Almeida, Paula Chaves
We report a case of metaplastic columnar epithelium in the mid-esophagus in a patient with history of caustic ingestion. A cardiac-type gastric phenotype, with early signs of intestinalization, was confirmed by immunohistochemistry studies (MUC5AC, MUC6, SOX2, and CDX2). Nonmetaplastic mucosa had histologic evidence of gastroesophageal reflux. In this case, esophageal reepithelization seems to have been modulated by acidic gastroesophageal reflux, which might activate transcription factors leading to phenotypic reprogramming of the regenerative epithelium...
October 5, 2016: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/27680978/gli1-a-potential-regulator-of-esophageal-cancer-stem-cell-is-identified-as-an-independent-adverse-prognostic-factor-in-esophageal-squamous-cell-carcinoma
#10
Zhaoting Yang, Yan Cui, Weidong Ni, Seokhyung Kim, Yanhua Xuan
PURPOSE: The hedgehog (Hh) pathway is involved in cancer stem cell (CSC) maintenance in various tumors. Glioma-associated oncogene homolog 1 (Gli1) is a key mediator of the Hh pathway; however, its expression and clinical significance in esophageal squamous cell carcinoma (ESCC) have not been reported. In this study, we aimed to reveal clinical significance of Gli1 expression in ESCC and further investigate the potential of Gli1 as a CSC regulator of ESCC by comparing its expression with expressions of other stemness genes in ESCC...
September 28, 2016: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/27678007/enteric-co-innervation-of-striated-muscle-in-the-esophagus-still-enigmatic
#11
Winfried L Neuhuber, Jürgen Wörl
The existence of a distinct ganglionated myenteric plexus between the two layers of the striated tunica muscularis of the mammalian esophagus has represented an enigma for quite a while. Although an enteric co-innervation of vagally innervated motor endplates in the esophagus has been suggested repeatedly, it was not possible until recently to demonstrate this dual innervation. Twenty-two years ago, we were able to demonstrate that motor endplates in the rat esophagus receive dual innervation from both vagal nerve fibers originating in the brain stem and from varicose enteric nerve fibers originating in the myenteric plexus...
September 28, 2016: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/27675014/hpv16-e6-e7-induces-cancer-stem-like-cell-phenotypes-and-chemoradioresistance-in-esophageal-squamous-cell-carcinoma-through-activation-of-pi3k-akt-signaling-pathway
#12
S Che, R Xi, S Pan, X Chen, B Hui, S Fu, J Guo, X Zhang
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27603913/cancer-testis-antigens-expression-regulation-tumor-invasion-and-use-in-immunotherapy-of-cancers
#13
Arash Salmaninejad, Mohammad Reza Zamani, Mehrnaz Pourvahedi, Zahra Golchehre, Ali Hosseini Bereshneh, Nima Rezaei
UNLABELLED: Cancer/testis antigens (CTAs) are named based on their expression pattern that is restricted in a number of normal and abnormal tissues. Tumor cells frequently express antigens whose expression is typically restricted to germ cells. Their unique expression pattern is guaranteed by precise epigenetic regulatory mechanisms. Because of their tumor-limited, high immunogenicity, and biased expression, discovery of these molecules provides unprecedented opportunities for further research and clinical development in the field of cancer diagnosis and immunotherapy...
October 2016: Immunological Investigations
https://www.readbyqxmd.com/read/27576349/sox1-is-correlated-to-stemness-state-regulator-sall4-through-progression-and-invasiveness-of-esophageal-squamous-cell-carcinoma
#14
Abolfazl Rad, Saeed Esmaeili Dizghandi, Mohammad Reza Abbaszadegan, Negin Taghechian, Maryam Najafi, Mohammad Mahdi Forghanifard
SOX1, as a tumor suppressor, play anti-tumorigenecity role in different cells and its expression is inhibited in a variety of cancers. The aim of this study was to evaluate SOX1 expression and its correlation with cancer stem cell (CSC) markers in ESCC. Using real time PCR, the relative comparative expression of SOX1 in 40 ESCC samples was assessed compared to related margin normal tissues, and its correlation with CSC markers including SALL4, SOX2, and MEIS1 was analyzed statistically. The results revealed significant under-expression of SOX1 in ESCC in significant correlation with different indices of poor prognosis including depth of tumor invasion (P=0...
December 15, 2016: Gene
https://www.readbyqxmd.com/read/27573773/transcommitment-paving-the-way-to-barrett-s-metaplasia
#15
David H Wang, Rhonda F Souza
Barrett's esophagus is the condition in which metaplastic columnar epithelium that predisposes to cancer development replaces stratified squamous epithelium in the distal esophagus. Potential sources for the cell or tissue of origin for metaplastic Barrett's epithelium are reviewed including native esophageal differentiated squamous cells, progenitor cells native to the esophagus located within the squamous epithelium or in the submucosal glands or ducts, circulating bone marrow-derived stem cells, and columnar progenitor cells from the squamocolumnar junction or the gastric cardia that proximally shift into the esophagus to fill voids left by damaged squamous epithelium...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27572636/possible-tumor-suppressive-role-of-the-mir-144-451-cluster-in-esophageal-carcinoma-as-determined-by-principal-component-regression-analysis
#16
Zhikui Gao, Ran Liu, Juan Liao, Miao Yang, Enchun Pan, Lihong Yin, Yuepu Pu
MicroRNA (miRNA) clusters are expressed universally across different types of organisms, and an accumulating number of studies have demonstrated that miRNA clusters function more efficiently compared with single miRNAs during the development of certain cancer types. miRNA clusters may have increased stability and reliability over individual miRNAs as diagnostic or therapeutic biomarkers. In the present study, the expression levels of mature miRNAs within the miR-144/451 cluster were examined using stem‑loop reverse transcription‑quantitative polymerase chain reaction in 102 patients pathologically diagnosed with esophageal carcinoma...
October 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27501171/mir-221-mediates-chemoresistance-of-esophageal-adenocarcinoma-by-direct-targeting-of-dkk2-expression
#17
Yan Wang, Yue Zhao, Andreas Herbst, Thomas Kalinski, Jiwei Qin, Xiaoyan Wang, Zhenzhong Jiang, Frank Benedix, Sabine Franke, Thomas Wartman, Peter Camaj, Walter Halangk, Frank T Kolligs, Karl W Jauch, Peter J Nelson, Christiane J Bruns
BACKGROUND: Chemoresistance is a main obstacle to effective esophageal cancer (EC) therapy. We hypothesize that altered expression of microRNAs (miRNAs) play a role in EC cancer progression and resistance to 5-fluorouracil (5-FU) based chemotherapeutic strategies. METHODS: Four pairs of esophageal adenocarcinoma (EAC) cell lines and corresponding 5-FU resistant variants were established. The expression levels of miRNAs previously shown to be involved in the general regulation of stem cell pathways were analyzed by qRT-PCR...
November 2016: Annals of Surgery
https://www.readbyqxmd.com/read/27489353/hpv16-e6-e7-induces-cancer-stem-like-cells-phenotypes-in-esophageal-squamous-cell-carcinoma-through-the-activation-of-pi3k-akt-signaling-pathway-in-vitro-and-in-vivo
#18
Ruxing Xi, Shupei Pan, Xin Chen, Beina Hui, Li Zhang, Shenbo Fu, Xiaolong Li, Xuanwei Zhang, Tuotuo Gong, Jia Guo, Xiaozhi Zhang, Shaomin Che
High-risk human papillomavirus (HPV), especially HPV16, correlates with cancerogenesis of human esophageal squamous cell carcinoma (ESCC) and we have reported that HPV16 related with a poor prognosis of ESCC patients in China. We aim to investigate the potential role and mechanism of HPV16 in ESCC development and progress. Our following researches demonstrated that ESCC cells which were stably transfected by HPV16 E6-E7 lentiviral vector showed a remarkable cancer stem-like cells (CSCs) phenotype, such as: migration, invasion, spherogenesis, high expression of CSCs marker in ESCC---p75NTR, chemoresistance, radioresistance, anti-apoptosis ability in vitro and cancerogenesis in vivo...
July 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27446428/gastrointestinal-tract-spindle-cell-tumors-with-interstitial-cells-of-cajal-prevalence-excluding-gastrointestinal-stromal-tumors
#19
So Jung Lee, Chung Su Hwang, Ahrong Kim, Kyungbin Kim, Kyung Un Choi
Leiomyomas and schwannomas of the gastrointestinal tract (GIT) are mainly comprised of spindle-shaped tumor cells and should always be differentiated from gastrointestinal stromal tumors (GISTs). Mast/stem cell growth factor receptor Kit (KIT) and discovered on GIST-1 (DOG1) are well-known diagnostic markers for the detection of a GIST by immunohistochemical staining. The aim of the present study was to assess the prevalence and significance of spindle cell tumors of the GIT with KIT- or DOG1-positive spindle-shaped cells, presumed to be interstitial cells of Cajal (ICCs), other than GISTs...
August 2016: Oncology Letters
https://www.readbyqxmd.com/read/27407092/downregulation-of-microrna-644a-promotes-esophageal-squamous-cell-carcinoma-aggressiveness-and-stem-cell-like-phenotype-via-dysregulation-of-pitx2
#20
Jia-Xing Zhang, Zhen-Hua Chen, Yi Xu, Jie-Wei Chen, Hui-Wen Weng, Miao Yun, Zou-San Zheng, Cui Chen, Bing-Li Wu, En-Min Li, Jian-Hua Fu, Sheng Ye, Dan Xie
PURPOSE: We previously reported the oncogenic role of paired-like homeodomain 2 (PITX2) in esophageal squamous cell carcinoma (ESCC). In this study, we aimed to identify the microRNA (miRNA) regulators of PITX2 and the mechanism underlying the pathogensis of ESCC. EXPERIMENTAL DESIGN: Using miRNA profiling and bioinformatics analyses, we identified miR-644a as a negative mediator of PITX2 in ESCC. A series of in vivo and in vitro assays were performed to confirm the effect of miR-644a on PITX2-mediated ESCC malignancy...
July 12, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
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