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Stem cell esophageal

Koichi Okamoto, Itasu Ninomiya, Yoshinao Ohbatake, Atsushi Hirose, Tomoya Tsukada, Shinichi Nakanuma, Seisho Sakai, Jun Kinoshita, Isamu Makino, Keishi Nakamura, Hironori Hayashi, Katsunobu Oyama, Masafumi Inokuchi, Hisatoshi Nakagawara, Tomoharu Miyashita, Tajima Hidehiro, Hiroyuki Takamura, Sachio Fushida, Tetsuo Ohta
Cancer stem cells (CSCs) have self-renewal and pluripotency capabilities and contribute to cancer progression and chemoresistance. It has been proposed that the treatment resistance and heterogeneity of CSCs are deeply involved in the prognosis of patients with esophageal squamous cell carcinoma (ESCC). The objective of this study was to identify the influence of the expression status of the CSC markers CD44 and CD133 on chemotherapeutic efficacy and prognosis in ESCC patients who underwent radical esophagectomy after neoadjuvant chemotherapy (NAC)...
September 28, 2016: Oncology Reports
Xiang Yuan, Jinyu Kong, Zhikun Ma, Na Li, Ruinuo Jia, Yiwen Liu, Fuyou Zhou, Qimin Zhan, Gang Liu, Shegan Gao
Our studies investigating the existence of tumor-initiating cell (TIC) populations in human esophageal squamous cell carcinoma (ESCC) had identified a subpopulation of cells isolated from ESCC patient-derived tumor specimens marked by an ALDH(bri+) phenotype bear stem cell-like features. Importantly, KDM4C, a histone demethylase was enhanced in ALDH(bri+) subpopulation, suggesting that strategies interfering with KDM4C may be able to target these putative TICs. In the present study, by genetic and chemical means, we demonstrated that, KDM4C blockade selectively decreased the ESCC ALDH(bri+) TICs population in vitro and specifically targeted the TICs in ALDH(bri+)-derived xenograft, retarding engraftment...
October 2016: Neoplasia: An International Journal for Oncology Research
Inês Rolim, Rita Vale Rodrigues, António Bettencourt, Rita Barros, Vânia Camilo, António Dias Pereira, Raquel Almeida, Paula Chaves
We report a case of metaplastic columnar epithelium in the mid-esophagus in a patient with history of caustic ingestion. A cardiac-type gastric phenotype, with early signs of intestinalization, was confirmed by immunohistochemistry studies (MUC5AC, MUC6, SOX2, and CDX2). Nonmetaplastic mucosa had histologic evidence of gastroesophageal reflux. In this case, esophageal reepithelization seems to have been modulated by acidic gastroesophageal reflux, which might activate transcription factors leading to phenotypic reprogramming of the regenerative epithelium...
October 5, 2016: International Journal of Surgical Pathology
Zhaoting Yang, Yan Cui, Weidong Ni, Seokhyung Kim, Yanhua Xuan
PURPOSE: The hedgehog (Hh) pathway is involved in cancer stem cell (CSC) maintenance in various tumors. Glioma-associated oncogene homolog 1 (Gli1) is a key mediator of the Hh pathway; however, its expression and clinical significance in esophageal squamous cell carcinoma (ESCC) have not been reported. In this study, we aimed to reveal clinical significance of Gli1 expression in ESCC and further investigate the potential of Gli1 as a CSC regulator of ESCC by comparing its expression with expressions of other stemness genes in ESCC...
September 28, 2016: Journal of Cancer Research and Clinical Oncology
Winfried L Neuhuber, Jürgen Wörl
The existence of a distinct ganglionated myenteric plexus between the two layers of the striated tunica muscularis of the mammalian esophagus has represented an enigma for quite a while. Although an enteric co-innervation of vagally innervated motor endplates in the esophagus has been suggested repeatedly, it was not possible until recently to demonstrate this dual innervation. Twenty-two years ago, we were able to demonstrate that motor endplates in the rat esophagus receive dual innervation from both vagal nerve fibers originating in the brain stem and from varicose enteric nerve fibers originating in the myenteric plexus...
September 28, 2016: Histochemistry and Cell Biology
S Che, R Xi, S Pan, X Chen, B Hui, S Fu, J Guo, X Zhang
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
Arash Salmaninejad, Mohammad Reza Zamani, Mehrnaz Pourvahedi, Zahra Golchehre, Ali Hosseini Bereshneh, Nima Rezaei
UNLABELLED: Cancer/testis antigens (CTAs) are named based on their expression pattern that is restricted in a number of normal and abnormal tissues. Tumor cells frequently express antigens whose expression is typically restricted to germ cells. Their unique expression pattern is guaranteed by precise epigenetic regulatory mechanisms. Because of their tumor-limited, high immunogenicity, and biased expression, discovery of these molecules provides unprecedented opportunities for further research and clinical development in the field of cancer diagnosis and immunotherapy...
October 2016: Immunological Investigations
Abolfazl Rad, Saeed Esmaeili Dizghandi, Mohammad Reza Abbaszadegan, Negin Taghechian, Maryam Najafi, Mohammad Mahdi Forghanifard
SOX1, as a tumor suppressor, play anti-tumorigenecity role in different cells and its expression is inhibited in a variety of cancers. The aim of this study was to evaluate SOX1 expression and its correlation with cancer stem cell (CSC) markers in ESCC. Using real time PCR, the relative comparative expression of SOX1 in 40 ESCC samples was assessed compared to related margin normal tissues, and its correlation with CSC markers including SALL4, SOX2, and MEIS1 was analyzed statistically. The results revealed significant under-expression of SOX1 in ESCC in significant correlation with different indices of poor prognosis including depth of tumor invasion (P=0...
December 15, 2016: Gene
David H Wang, Rhonda F Souza
Barrett's esophagus is the condition in which metaplastic columnar epithelium that predisposes to cancer development replaces stratified squamous epithelium in the distal esophagus. Potential sources for the cell or tissue of origin for metaplastic Barrett's epithelium are reviewed including native esophageal differentiated squamous cells, progenitor cells native to the esophagus located within the squamous epithelium or in the submucosal glands or ducts, circulating bone marrow-derived stem cells, and columnar progenitor cells from the squamocolumnar junction or the gastric cardia that proximally shift into the esophagus to fill voids left by damaged squamous epithelium...
2016: Advances in Experimental Medicine and Biology
Zhikui Gao, Ran Liu, Juan Liao, Miao Yang, Enchun Pan, Lihong Yin, Yuepu Pu
MicroRNA (miRNA) clusters are expressed universally across different types of organisms, and an accumulating number of studies have demonstrated that miRNA clusters function more efficiently compared with single miRNAs during the development of certain cancer types. miRNA clusters may have increased stability and reliability over individual miRNAs as diagnostic or therapeutic biomarkers. In the present study, the expression levels of mature miRNAs within the miR-144/451 cluster were examined using stem‑loop reverse transcription‑quantitative polymerase chain reaction in 102 patients pathologically diagnosed with esophageal carcinoma...
October 2016: Molecular Medicine Reports
Yan Wang, Yue Zhao, Andreas Herbst, Thomas Kalinski, Jiwei Qin, Xiaoyan Wang, Zhenzhong Jiang, Frank Benedix, Sabine Franke, Thomas Wartman, Peter Camaj, Walter Halangk, Frank T Kolligs, Karl W Jauch, Peter J Nelson, Christiane J Bruns
BACKGROUND: Chemoresistance is a main obstacle to effective esophageal cancer (EC) therapy. We hypothesize that altered expression of microRNAs (miRNAs) play a role in EC cancer progression and resistance to 5-fluorouracil (5-FU) based chemotherapeutic strategies. METHODS: Four pairs of esophageal adenocarcinoma (EAC) cell lines and corresponding 5-FU resistant variants were established. The expression levels of miRNAs previously shown to be involved in the general regulation of stem cell pathways were analyzed by qRT-PCR...
November 2016: Annals of Surgery
Ruxing Xi, Shupei Pan, Xin Chen, Beina Hui, Li Zhang, Shenbo Fu, Xiaolong Li, Xuanwei Zhang, Tuotuo Gong, Jia Guo, Xiaozhi Zhang, Shaomin Che
High-risk human papillomavirus (HPV), especially HPV16, correlates with cancerogenesis of human esophageal squamous cell carcinoma (ESCC) and we have reported that HPV16 related with a poor prognosis of ESCC patients in China. We aim to investigate the potential role and mechanism of HPV16 in ESCC development and progress. Our following researches demonstrated that ESCC cells which were stably transfected by HPV16 E6-E7 lentiviral vector showed a remarkable cancer stem-like cells (CSCs) phenotype, such as: migration, invasion, spherogenesis, high expression of CSCs marker in ESCC---p75NTR, chemoresistance, radioresistance, anti-apoptosis ability in vitro and cancerogenesis in vivo...
July 30, 2016: Oncotarget
So Jung Lee, Chung Su Hwang, Ahrong Kim, Kyungbin Kim, Kyung Un Choi
Leiomyomas and schwannomas of the gastrointestinal tract (GIT) are mainly comprised of spindle-shaped tumor cells and should always be differentiated from gastrointestinal stromal tumors (GISTs). Mast/stem cell growth factor receptor Kit (KIT) and discovered on GIST-1 (DOG1) are well-known diagnostic markers for the detection of a GIST by immunohistochemical staining. The aim of the present study was to assess the prevalence and significance of spindle cell tumors of the GIT with KIT- or DOG1-positive spindle-shaped cells, presumed to be interstitial cells of Cajal (ICCs), other than GISTs...
August 2016: Oncology Letters
Jia-Xing Zhang, Zhen-Hua Chen, Yi Xu, Jie-Wei Chen, Hui-Wen Weng, Miao Yun, Zou-San Zheng, Cui Chen, Bing-Li Wu, En-Min Li, Jian-Hua Fu, Sheng Ye, Dan Xie
PURPOSE: We previously reported the oncogenic role of paired-like homeodomain 2 (PITX2) in esophageal squamous cell carcinoma (ESCC). In this study, we aimed to identify the microRNA (miRNA) regulators of PITX2 and the mechanism underlying the pathogensis of ESCC. EXPERIMENTAL DESIGN: Using miRNA profiling and bioinformatics analyses, we identified miR-644a as a negative mediator of PITX2 in ESCC. A series of in vivo and in vitro assays were performed to confirm the effect of miR-644a on PITX2-mediated ESCC malignancy...
July 12, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Eihab Abdelfatah, Zachary Kerner, Nainika Nanda, Nita Ahuja
Epigenetics is a relatively recent field of molecular biology that has arisen over the past 25 years. Cancer is now understood to be a disease of widespread epigenetic dysregulation that interacts extensively with underlying genetic mutations. The development of drugs targeting these processes has rapidly progressed; with several drugs already FDA approved as first-line therapy in hematological malignancies. Gastrointestinal (GI) cancers possess high degrees of epigenetic dysregulation, exemplified by subtypes such as CpG island methylator phenotype (CIMP), and the potential benefit of epigenetic therapy in these cancers is evident...
July 2016: Therapeutic Advances in Gastroenterology
Xiang Xue, Chunguang Li, Yan Yan, Xilong Lang, Zhiyun Xu, Hezhong Chen, Hao Zhang
OBJECTIVE: To investigate the efficacy of MSCs autografting with fibrin sealant could for the closure of cervical anastomotic fistula. METHODS: Twenty-one healthy New Zealand rabbits were involved and randomly assigned to treatment group (n = 12) or control group (n = 9). After the bone marrow were aspirated, the MSCs were isolated, purified and labelled by transfection of Lenti. GFP. The rabbit models of cervical esophageal anastomotic fistula were established by leaving a caliber rubber tube inside esophageal lumen...
December 15, 2015: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Jing He, Mingxia Zhou, Xinfeng Chen, Dongli Yue, Li Yang, Guohui Qin, Zhen Zhang, Qun Gao, Dan Wang, Chaoqi Zhang, Lan Huang, Liping Wang, Bin Zhang, Jane Yu, Yi Zhang
BACKGROUND: Growing evidence suggests that SALL4 plays a vital role in tumor progression and metastasis. However, the molecular mechanism of SALL4 promoting esophageal squamous cell carcinoma (ESCC) remains to be elucidated. METHODS: The gene and protein expression profiles- were examined by using quantitative real-time PCR, immunohistochemistry and western blotting. Small hairpin RNA was used to evaluate the role of SALL4 both in cell lines and in animal models...
2016: Journal of Experimental & Clinical Cancer Research: CR
Chao He, Lun Li, Xuan Guan, Li Xiong, Xiongying Miao
PURPOSE: To review mechanisms underlying mutant p53 (mutp53) gain of function (GOF) and mutp53-induced chemoresistance, and to investigate the role of mutp53 in response to clinical chemotherapy. METHODS: We searched the PubMed database for clinical studies from the past decade, including data evaluating the impact of mutp53 in clinical chemotherapy response. RESULTS: Interactions between mutp53 and transcriptional factors, proteins or DNA structures, as well as epigenetic regulation, contribute to mutp53 GOF...
June 21, 2016: Chemotherapy
Saffiyeh Saboor-Maleki, Fatemeh B Rassouli, Maryam M Matin, Mehrdad Iranshahi
The high incidence of esophageal squamous cell carcinoma has been reported in selected ethnic populations including North of Iran. Low survival rate of esophageal carcinoma is partially due to the presence of stem-like cancer cells with chemotherapy resistance. In the current study, we aimed to determine the effects of auraptene, an interesting dietary coumarin with various biological activities, on malignant properties of stem-like esophageal squamous cell carcinoma, in terms of sensitivity to anticancer drugs and expression of specific markers...
May 20, 2016: Technology in Cancer Research & Treatment
Dan-Dan Xu, Peng-Jun Zhou, Ying Wang, Li Zhang, Wu-Yu Fu, Bi-Bo Ruan, Hai-Peng Xu, Chao-Zhi Hu, Lu Tian, Jin-Hong Qin, Sheng Wang, Xiao Wang, Yi-Cheng Li, Qiu-Ying Liu, Zhe Ren, Rong Zhang, Yi-Fei Wang
BACKGROUND: Recent studies have suggested that cancer cells contain subpopulations that can initiate tumor growth, self-renew, and maintain tumor cell growth. However, for esophageal cancer cells, the relationship between STAT3, microRNAs and cancer stem cells remains unclear. METHODS: Serum-free culture was used to enrich esophageal cancer stem-like cells (ECSLC). Flow cytometry determined the proportion of ECSLC. qPCR were performed to examine expression level of stemness factors, mesenchymal markers, ATP-binding cassette (ABC) transporters, STAT3, miR-181b, CYLD...
2016: Molecular Cancer
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