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M2 polarization

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https://www.readbyqxmd.com/read/28635229/-macrophage-colony-stimulating-factor-enhances-non-small-cell-lung-cancer-invasion-and-metastasis-by-promoting-macrophage-m2-polarization
#1
Y J Li, L Yang, L P Wang, Y Zhang
Objective: To investigate the key cytokine which polarizes M2 macrophages and promotes invasion and metastasis in non-small cell lung cancer (NSCLC). Methods: After co-culture with A549 cells in vitro, the proportion of CD14(+) CD163(+) M2 macrophages in monocytes and macrophage colony stimulating factor (M-CSF) levels in culture supernatant were detected by flow cytometry, ELISA assay and real-time qPCR, respectively. The effects of CD14(+) CD163(+) M2 macrophages on invasion of A549 cells and angiogenesis of HUVEC cells were measured by transwell assay and tubule formation assay, respectively...
June 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28631559/pkc%C3%AE-in-prostate-cancer-cells-represses-the-recruitment-and-m2-polarization-of-macrophages-in-the-prostate-cancer-microenvironment
#2
Hui-Hui Fan, Ling Li, Yu-Ming Zhang, Jie Yang, Mao-Cheng Li, Fang-Yin Zeng, Fan Deng
Tumor-associated macrophages are key regulators of the complex interplay between tumor and tumor microenvironment. M2 Macrophages, one type of tumor-associated macrophages, are involved in prostate cancer growth and progression. Protein kinase C zeta has been shown to suppress prostate cancer cell growth, invasion, and metastasis as a tumor suppressor; however, its role in chemotaxis and activation of tumor-associated macrophages remains unclear. Here, we investigated the role of protein kinase C zeta of prostate cancer cells in regulation of macrophage chemotaxis and M2 phenotype activation...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28629783/grp78-plays-an-integral-role-in-tumor-cell-inflammation-related-migration-induced-by-m2-macrophages
#3
Lichao Zhang, Zongwei Li, Guobin Ding, Xiaoqin La, Peng Yang, Zhuoyu Li
Macrophages are the main immune-competent cells that infiltrate in tumors. Tumor-associated macrophages (TAMs), termed M2 macrophages, facilitate tumor progress and promote metastasis. However, M2 macrophages always display an immunosuppressive phenotype, which is not in accordance with the tumor inflammatory microenvironment and inflammation-related metastasis. In this study, we established a macrophage polarization model with human monocytes and found that the conditioned medium from M2 macrophages increased GRP78 expression in tumor cells and facilitated tumor cell migration...
June 16, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28629744/mannose-receptor-modulates-macrophage-polarization-and-allergic-inflammation-through-mir-511-3p
#4
Yufeng Zhou, Danh C Do, Faoud T Ishmael, Mario Leonardo Squadrito, Ho Man Tang, Ho Lam Tang, Man-Hsun Hsu, Lipeng Qiu, Changjun Li, Yongqing Zhang, Kevin G Becker, Mei Wan, Shau-Ku Huang, Peisong Gao
BACKGROUND: Mannose receptor (MRC1/CD206) has been suggested to mediate allergic sensitization and asthma to multiple glyco-allergens, including cockroach allergens. OBJECTIVE: Determine the existence of a protective mechanism through which MRC1 limits allergic inflammation through its intronic miR-511-3p. METHODS: We examined the MRC1-mediated cockroach allergen uptake by lung macrophages and lung inflammation using C57BL/6 wild-type (WT) and Mrc1(-/-) mice...
June 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28627621/identification-of-different-macrophage-subpopulations-with-distinct-activities-in-a-mouse-model-of-oxygen-induced-retinopathy
#5
Yanji Zhu, Ling Zhang, Qing Lu, Yushuo Gao, Yujuan Cai, Ailing Sui, Ting Su, Xi Shen, Bing Xie
The aim of the present study was to characterize the phenotypic shift, quantity and role changes in different subgroups of retinal macrophages in a mouse model of oxygen-induced retinopathy (OIR). The mRNA expression levels of macrophage M1 and M2 subgroup marker genes and polarization-associated genes were analyzed by RT-qPCR. The number of M1 and M2 macrophages in our mouse model of OIR was analyzed by flow cytometry at different time points during the progression of OIR. Immunofluorescence whole mount staining of the retinas of mice with OIR was performed at different time points to examine the influx of macrophages, as well as the morphological characteristics and roles of M1 and M2 macrophages...
June 13, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28626216/therapeutic-effects-of-csf1r-blocking-antibodies-in-multiple-myeloma
#6
Q Wang, Y Lu, R Li, Y Jiang, Y Zheng, J Qian, E Bi, C Zhang, J Hou, S Wang, Q Yi
Our previous studies showed that macrophages (MФs), especially myeloma-associated MФs (MAMs) induce chemoresistance in human myeloma. Here we explored the potential of targeting MФs, by using colony-stimulating factor 1 receptor (CSF1R)-blocking mAbs, to treat myeloma. Our results showed that CSF1R blockade specifically inhibited the differentiation, proliferation and survival of murine M2 MФs and MAMs, and repolarized MAMs towards M1-like MФs in vitro. CSF1R blockade alone inhibited myeloma growth in vivo, by partially depleting MAMs, polarizing MAMs to the M1 phenotype, and inducing a tumor-specific cytotoxic CD4(+) T cell response...
June 19, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/28624450/%C3%AE-elemene-inhibits-tumor-promoting-effect-of-m2-macrophages-in-lung-cancer
#7
Xiaomu Yu, Maoyi Xu, Na Li, Zongjuan Li, Hongye Li, Shujuan Shao, Kun Zou, Lijuan Zou
Macrophages in tumor are mostly M2-polarized and have been reported to promote tumorigenesis, which are also defined as tumor-associated macrophages (TAMs). β-elemene has therapeutic effects against several cancers, however, it remains unknown whether β-elemene could inhibit cancer by targeting TAMs. Herein, we examined the effect of β-elemene on macrophages to elucidate a novel mechanism of β-elemene in tumor therapy. We showed that the conditioned medium of M2 macrophages promoted lung cancer cells to migration, invasion and epithelial mesenchymal transition, which could be inhibited by β-elemene...
June 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28623741/oxidized-ldl-activated-eosinophil-polarize-macrophage-phenotype-from-m2-to-m1-through-activation-of-cd36-scavenger-receptor
#8
Minghui Qin, Lai Wang, Fuqiang Li, Mingjie Yang, Lei Song, Fang Tian, Ada Yukht, Prediman K Shah, Marc E Rothenberg, Behrooz G Sharifi
BACKGROUND AND AIMS: Inflammation, particularly innate immunity, plays an important role in cardiovascular diseases. The aim of this study was to investigate whether atherogenic determinants such as oxidized LDL modulate the phenotype of eosinophils. METHODS: Cultured eosinophils were treated with oxidized LDL and the expression of selective inflammatory and anti-inflammatory cytokines was determined. In addition, the eosinophil receptor and signaling that mediate these events were identified...
May 20, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28623721/macrophage-phenotypic-mechanomodulation-of-enhancing-bone-regeneration-by-superparamagnetic-scaffold-upon-magnetization
#9
Suisui Hao, Jie Meng, Yu Zhang, Jian Liu, Xin Nie, Fengxin Wu, Yanlian Yang, Chen Wang, Ning Gu, Haiyan Xu
Macrophages are involved in all phases of scaffold induced tissue regeneration, orchestrating the transition from an inflammatory to regenerative phenotype to guide all other cell types to complete the wound healing process when a tissue defect advances beyond the critical size. Therefore, harnessing macrophages by scaffolds is important for facilitating tissue regeneration in situ. In this work we utilized the superparamagnetic scaffold upon magnetization as a mechanostimulation platform to apply forces directly to macrophages grown in the scaffold, aiming to figure out whether the functions of macrophages related to bone tissue regeneration can be mechanomodulated and to elucidate the underlying mechanisms...
June 9, 2017: Biomaterials
https://www.readbyqxmd.com/read/28623715/diosgenin-glucoside-provides-neuroprotection-by-regulating-microglial-m1-polarization
#10
Shaoxia Wang, Fujiang Wang, Hongyun Yang, Ruilin Li, Hong Guo, Limin Hu
The selective suppression of inflammatory factors in activated microglia, rather than totally inhibiting their activation, might be an effective means of slowing the progression of certain neurodegenerative diseases. Diosgenin glucoside (Dios) is a saponin compound extracted from Tritulus terrestris L. We found that Dios suppressed the synthesis of molecules that promote inflammation (M1 markers, such as NO, IL-6, and TNF-α) in rat microglia and BV-2 cells induced with lipopolysaccharides (LPS). In contrast, Dios had no effects on the cellular production of anti-inflammatory factors (M2 markers, such as IL-10, IL-1Rα and CD206) in LPS and IL-4 treated microglia...
June 14, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28623530/participation-of-tumor-associated-myeloid-cells-in-progression-of-amelanotic-melanoma-rmm-tumor-line-in-f344-rats-with-particular-reference-to-mhc-class-ii-and-cd163-expressing-cells
#11
A Bondoc, H M Golbar, M Pervin, C Katou-Ichikawa, M Tanaka, T Izawa, M Kuwamura, J Yamate
Tumor progression is often influenced by infiltration of myeloid cells; depending on the M1- or M2-like activation status, these cells may have either inhibitory or promoting effects on tumor growth. We investigated the properties of tumor-associated myeloid cells in a previously established homotransplantable amelanotic melanoma (RMM tumor line) in F344 rats. RMM tumor nodules were allowed to reach the sizes of 0.5, 1, 2 and 3 cm, respectively. Immunohistochemistry and flow cytometry was performed for macrophage markers CD68 and CD163, and for the antigen-presenting cell marker, MHC class II...
June 16, 2017: Cancer Microenvironment: Official Journal of the International Cancer Microenvironment Society
https://www.readbyqxmd.com/read/28621777/polar-metal-formate-frameworks-templated-with-1-2-diaminoethane-water-assemblies-showing-ferromagnetic-and-ferroelectric-properties
#12
Monika Trzebiatowska, Bartosz Zarychta, Adam Pikul, Mirosław Mączka, Paulina Peksa, Ryszard Poprawski
A set of five novel formate frameworks templated with assemblies comprising diprotonated 1,2-diaminoethane (DAE) and a water molecule of the formula: [NH3(CH2)2NH3]M2(HCOO)6·H2O, where M = Mg, Mn, Co, Ni, Zn, has been synthesized. Four compounds crystallize in the polar R3 space group and one in the chiral P6322 space group (Ni-analog) at room temperature. The polyammonium-water assemblies, mutually joined by hydrogen bonds, fill the cavities of the frameworks and are disordered in the three latter compounds...
June 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28621212/methamphetamine-causes-neurotoxicity-by-promoting-polarization-of-macrophages-and-inflammatory-response
#13
X Li, F Wu, L Xue, B Wang, J Li, Y Chen, T Chen
Macrophages, especially their activation state, are closely related to the progression of neurotoxicity. Classically activated macrophages (M1) are proinflammatory effectors, while alternatively activated macrophages (M2) exhibit anti-inflammatory properties. As a powerful addictive psychostimulant drug, coupled with its neurotoxicity, methamphetamine (Meth) abuse may lead to long-lasting abnormalities in the neuronal system. The present study investigated the effect of Meth at subtoxic concentration on macrophage activation state and its underlying toxicity to neuronal cells...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28620389/2-deoxy-d-glucose-treatment-decreases-anti-inflammatory-m2-macrophage-polarization-in-mice-with-tumor-and-allergic-airway-inflammation
#14
Qingjie Zhao, Zhulang Chu, Linnan Zhu, Tao Yang, Peng Wang, Fang Liu, Ying Huang, Fang Zhang, Xiaodong Zhang, Wenjun Ding, Yong Zhao
As important effector cells in inflammation, macrophages can be functionally polarized into either inflammatory M1 or alternatively activated anti-inflammatory M2 phenotype depending on surroundings. The key roles of glycolysis in M1 macrophage polarization have been well defined. However, the relationship between glycolysis and M2 polarized macrophages is still poorly understood. Here, we report that 2-deoxy-d-glucose (2-DG), an inhibitor of the glycolytic pathway, markedly inhibited the expressions of Arg, Ym-1, Fizz1, and CD206 molecules, the hall-markers for M2 macrophages, during macrophages were stimulated with interleukin 4...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28620274/influence-of-estrogen-modulation-on-glia-activation-in-a-murine-model-of-parkinson-s-disease
#15
Francesca Siani, Rosaria Greco, Giovanna Levandis, Cristina Ghezzi, Francesca Daviddi, Chiara Demartini, Elisabetta Vegeto, Marie-Thérèse Fuzzati-Armentero, Fabio Blandini
Epidemiological data suggest a sexual dimorphism in Parkinson disease (PD), with women showing lower risk of developing PD. Vulnerability of the nigrostriatal pathway may be influenced by exposure to estrogenic stimulation throughout fertile life. To further address this issue, we analyzed the progression of nigrostriatal damage, microglia and astrocyte activation and microglia polarization triggered by intrastriatal injection of dopaminergic neurotoxin 6-hydroxydopamine (6-OHDA) in male, female and ovariectomized (OVX) mice, as well as in OVX mice supplemented with 17βestradiol (OVX+E)...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28615615/stat6-upregulation-promotes-m2-macrophage-polarization-to-suppress-atherosclerosis
#16
Min Gong, Xiaozhen Zhuo, Aiqun Ma
BACKGROUND Macrophages are highly heterogeneous and plastic cells that are involved in all stages of atherogenesis. They can undergo polarization by shifting between M1 and M2 functional phenotypes. However, the role of macrophage polarization and the molecular mechanism in modulating atherosclerotic plaque stability remain incompletely understood. Our study investigated the role of STAT6 in regulating macrophage phenotypes to affect atherosclerotic plaque stability. MATERIAL AND METHODS A murine atherosclerosis model with vulnerable plaques was induced with high-cholesterol diet and PCCP surgeries in ApoE-/- mice...
June 15, 2017: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/28615091/-detection-and-analysis-of-phenotypes-of-tumor-associated-macrophages-in-mouse-model-of-spontaneous-breast-cancer
#17
Xiaojie Ren, Peng Ren, Song Luo, Quanbo Ji, Meng Xu, Ning Lu, Yan Wang
Objective To detect the phenotypic conversion of tumor-associated macrophages (TAMs) through analyzing the expression levels of the polarization-related genes. Methods We identified the spontaneous breast cancer mice by genotyping and characterized them into the early stage and the advanced stage groups according to their tumor size. Single cell suspension of the tumor tissues were obtained by mechanical methods and TAMs of different stages were sorted by flow cytometry. We measured the mRNA levels of M1 macrophages-related genes IL-1β, IL-27, IL-6, CD80, CD86 and M2 macrophages-related genes arginase 1 (Arg1), IL-10, interleukin 4 receptor α (IL-4Rα), macrophage mannose receptor 1 (Mrc1), chitinase-like 3 (Chil3/Ym1) by real-time quantitative PCR in order to analyze the phenotypic conversion of TAMs during the tumor progression...
June 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28611100/ampk-activation-enhances-the-anti-atherogenic-effects-of-high-density-lipoproteins-in-apoe-mice
#18
Ang Ma, Jing Wang, Liu Yang, Yuanyuan An, Haibo Zhu
HDL plays crucial roles at multiple stages of the pathogenesis of atherosclerosis. AMP-activated protein kinase (AMPK) is a therapeutic candidate for the treatment of cardiovascular disease. However, the effect of AMPK activation on HDL functionality has not been established in vivo. We assessed the effects of pharmacological AMPK activation using A-769662, AICAR, metformin, and IMM-H007 on the atheroprotective functions of HDL in apoE-deficient (apoE-/-) mice fed with a high fat diet. After administration, although there no changes in serum lipid levels among the groups...
June 13, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28609713/nicorandil-modulated-macrophages-activation-and-polarization-via-nf-%C3%AE%C2%BAb-signaling-pathway
#19
Fengyun Zhang, Yongli Xuan, Jinjin Cui, Xinxin Liu, Zhiying Shao, Bo Yu
Nicorandil, a drug with both nitrate-like and ATP-sensitive potassium (KATP) channel-activating properties, has been well demonstrated in various aspects of myocardial infarction (MI), especially in inhibiting cell apoptosis and increasing coronary flow. However, the role of nicorandil in regulating inflammation and angiogenesis following myocardial infarction is still unrevealed. In the present study, we explored the effect of nicorandil on macrophage phenotype transition and inflammation regulation and the potential underlying mechanisms...
June 10, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28606793/mir-20b-inhibits-mycobacterium-tuberculosis-induced-inflammation-in-the-lung-of-mice-through-targeting-nlrp3
#20
Jun Lou, Yongli Wang, Zhimin Zhang, Weiqiang Qiu
Tuberculosis (TB) is one of immune-related disorders. Although dysregulation of miRNAs has been implicated in innate and adaptive immune response, the specific effect of miR-20b on TB has not been documented. In this study, downregulation of miR-20b and upregulation of NLRP3 were observed in macrophages of TB patients, increased levels of inflammatory factors secretion were observed in serum of TB patients. Next, we established a TB mice model by using M. tuberculosis infection and intravenous injection of miR-20b mimic...
June 9, 2017: Experimental Cell Research
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