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Aspergillus fumigatus

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https://www.readbyqxmd.com/read/29930111/response-to-comment-on-sterilizing-immunity-in-the-lung-relies-on-targeting-fungal-apoptosis-like-programmed-cell-death
#1
Neta Shlezinger, Henriette Irmer, Sourabh Dhingra, Sarah R Beattie, Robert A Cramer, Gerhard H Braus, Amir Sharon, Tobias M Hohl
Aouacheria et al question the interpretation of contemporary assays to monitor programmed cell death with apoptosis-like features (A-PCD) in Aspergillus fumigatus Although our study focuses on fungal A-PCD for host immune surveillance and infectious outcomes, the experimental approach incorporates multiple independent A-PCD markers and genetic manipulations based on fungal rather than mammalian orthologs to circumvent the limitations associated with any single approach.
June 22, 2018: Science
https://www.readbyqxmd.com/read/29930109/comment-on-sterilizing-immunity-in-the-lung-relies-on-targeting-fungal-apoptosis-like-programmed-cell-death
#2
Abdel Aouacheria, Kyle W Cunningham, J Marie Hardwick, Zdena Palková, Ted Powers, Fedor F Severin, Libuše Váchová
Shlezinger et al (Reports, 8 September 2017, p. 1037) report that the common fungus Aspergillus fumigatus , a cause of aspergillosis, undergoes caspase-dependent apoptosis-like cell death triggered by lung neutrophils. However, the technologies they used do not provide reliable evidence that fungal cells die via a protease signaling cascade thwarted by a fungal caspase inhibitor homologous to human survivin.
June 22, 2018: Science
https://www.readbyqxmd.com/read/29927507/utility-of-recombinant-aspergillus-fumigatus-antigens-in-the-diagnosis-of-allergic-bronchopulmonary-aspergillosis-a-systematic-review-and-diagnostic-test-accuracy-meta-analysis
#3
Valliappan Muthu, Inderpaul Singh Sehgal, Sahajal Dhooria, Ashutosh N Aggarwal, Ritesh Agarwal
BACKGROUND: The role of recombinant A.fumigatus (rAsp) antigens in the diagnosis of allergic bronchopulmonary aspergillosis (ABPA) has not been systematically evaluated. Herein, we evaluate the utility of recombinant A.fumigatus (rAsp) antigens in diagnosing ABPA. METHODS: We systematically reviewed the PubMed, EmBase, and Scopus databases for studies evaluating rAsp antigens in ABPA. The QUADAS-2 tool and the GRADE approach were used to assess the risk of bias and the quality of evidence, respectively...
June 21, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29925597/nanoparticle-decoration-impacts-airborne-fungal-pathobiology
#4
Dana Westmeier, Djamschid Solouk-Saran, Cecilia Vallet, Svenja Siemer, Dominic Docter, Hermann Götz, Linda Männ, Anja Hasenberg, Angelina Hahlbrock, Kathrin Erler, Christoph Reinhardt, Oliver Schilling, Sven Becker, Matthias Gunzer, Mike Hasenberg, Shirley K Knauer, Roland H Stauber
Airborne fungal pathogens, predominantly Aspergillus fumigatus , can cause severe respiratory tract diseases. Here we show that in environments, fungal spores can already be decorated with nanoparticles. Using representative controlled nanoparticle models, we demonstrate that various nanoparticles, but not microparticles, rapidly and stably associate with spores, without specific functionalization. Nanoparticle-spore complex formation was enhanced by small nanoparticle size rather than by material, charge, or "stealth" modifications and was concentration-dependently reduced by the formation of environmental or physiological biomolecule coronas...
June 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29922626/investigation-an-antifungal-activity-of-diclofenac-sodium-against-hyphae-formation-in-aspergillus-fumigatus-with-attention-to-the-expression-of-ef-1-gene
#5
Sanaz Nargesi, Sassan Rezaie
No abstract text is available yet for this article.
May 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29922270/-aspergillus-fumigatus-inhibits-pseudomonas-aeruginosa-in-co-culture-implications-of-a-mutually-antagonistic-relationship-on-virulence-and-inflammation-in-the-cf-airway
#6
Emma Reece, Sean Doyle, Peter Greally, Julie Renwick, Siobhán McClean
Many cystic fibrosis (CF) airway infections are considered to be polymicrobial and microbe-microbe interactions may play an important role in disease pathology. Pseudomonas aeruginosa and Aspergillus fumigatus are the most prevalent bacterial and fungal pathogens isolated from the CF airway, respectively. We have previously shown that patients co-colonized with these pathogens had comparable outcomes to those chronically colonized with P. aeruginosa . Our objective was to examine the interactions between A...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29920796/diagnostic-cut-off-of-a-fumigatus-specific-igg-in-the-diagnosis-of-chronic-pulmonary-aspergillosis
#7
Inderpaul Singh Sehgal, Hansraj Choudhary, Sahajal Dhooria, Ashutosh Nath Aggarwal, Mandeep Garg, Arunaloke Chakrabarti, Ritesh Agarwal
A.fumigatus-specific IgG is pivotal in making the diagnosis of chronic pulmonary aspergillosis (CPA). However, the cut-off value for A.fumigatus-specific IgG remains unknown. We included consecutive treatment-naïve subjects with chronic cavitary pulmonary aspergillosis (CCPA, cases). The controls were subjects with treated pulmonary tuberculosis, who had residual radiological abnormality and minimal symptoms. The diagnosis of CCPA was based on consistent clinicoradiological features along with demonstration of Aspergillus infection (growth of Aspergillus in sputum or bronchoalveolar lavage fluid [BALF] culture; serum or BALF galactomannan index>0...
June 19, 2018: Mycoses
https://www.readbyqxmd.com/read/29914956/cas9-crispr-genome-editing-to-demonstrate-the-contribution-of-cyp51a-gly138ser-to-azole-resistance-in-aspergillus-fumigatus
#8
Takashi Umeyama, Yuta Hayashi, Hisaki Shimosaka, Tatsuya Inukai, Satoshi Yamagoe, Shogo Takatsuka, Yasutaka Hoshino, Minoru Nagi, Shigeki Nakamura, Katsuhiko Kamei, Kenji Ogawa, Yoshitsugu Miyazaki
A pan-azole-resistant Aspergillus fumigatus strain with the cyp51A mutations Gly138Ser and Asn248Lys was isolated from a patient receiving long-term voriconazole treatment. PCR fragments containing cyp51A with the mutations were introduced along with Cas9 protein and single-guide RNA into the azole-resistant/susceptible strains. Recombinant strains showed increased susceptibility via the replacement of Ser138 by glycine. Genetic recombination, which has been hampered thus far in clinical isolates, can now be achieved using Cas9/CRISPR genome editing...
June 18, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29911293/improved-and-adopted-murine-models-to-combat-pulmonary-aspergillosis
#9
REVIEW
Gaspar Banfalvi
The insufficient basic and clinical knowledge about invasive mold infections necessitated to review aspergillosis rodent models. The scope of this review has two major aspects. (1) It briefly summarizes Aspergillus toxicoses, the adverse effects of Aspergillus mycotoxins, the virulence factors of Aspergillus fumigatus, and how mild Aspergillus infections can turn to immunosuppressive diseases, ultimately to lethal invasive pulmonary aspergillosis. (2) The second major aspect of the review deals with earlier and recent murine models of pulmonary aspergillosis...
June 17, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29907871/c3a-receptor-antagonism-as-a-novel-therapeutic-target-for-chronic-rhinosinusitis
#10
Jennifer K Mulligan, Kunal Patel, Tucker Williamson, Nicholas Reaves, William Carroll, Sarah E Stephenson, Peng Gao, Richard R Drake, Benjamin A Neely, Stephen Tomlinson, Rodney J Schlosser, Carl Atkinson
Chronic rhinosinusitis with nasal polyps (CRSwNP) is an inflammatory disease with an unknown etiology. Recent studies have implicated the complement system as a potential modulator of disease immunopathology. We performed proteomic pathway enrichment analysis of differentially increased proteins, and found an enrichment of complement cascade pathways in the nasal mucus of individuals with CRSwNP as compared to control subjects. Sinonasal mucus levels of complement 3 (C3) correlated with worse subjective disease severity, whereas no significant difference in systemic C3 levels could be determined in plasma samples...
June 15, 2018: Mucosal Immunology
https://www.readbyqxmd.com/read/29897360/systems-impact-of-zinc-chelation-by-the-epipolythiodioxopiperazine-dithiol-gliotoxin-in-aspergillus-fumigatus-a-new-direction-in-natural-product-functionality
#11
Aliabbas A Saleh, Gary W Jones, Frances C Tinley, Stephen F Delaney, Sahar H Alabbadi, Keith Fenlon, Sean Doyle, Rebecca A Owens
The non-ribosomal peptide gliotoxin, which autoinduces its own biosynthesis, has potent anti-fungal activity, especially in the combined absence of the gliotoxin oxidoreductase GliT and bis-thiomethyltransferase GtmA. Dithiol gliotoxin (DTG) is a substrate for both of these enzymes. Herein we demonstrate that DTG chelates Zn2+ (m/z 424.94), rapidly chelates Zn2+ from Zn(4-(2-pyridylazo)-resorcinol) (Zn(PAR)2) and also inhibits a Zn2+-dependent alkaline phosphatase (AP). Zn2+ addition rescues AP function following DTG-associated inhibition, and pre-incubation of DTG with Zn2+ completely protects AP activity...
June 13, 2018: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29897295/neoteric-environmental-detoxification-of-organic-pollutants-and-pathogenic-microbes-via-green-synthesized-zno-nanoparticles
#12
Shaan Bibi Jaffri, Khuram Shahzad Ahmad
Present study has for the first time reported Prunus cerasifera leaf extract mediated zinc oxide nanoparticles in a green and one pot synthetic mode without utilization of any chemical reducing agents. Synthesized nanoparticles were analyzed by ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), fourier transmission infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). UV-Vis peak was detected at 380 nm due to surface plasmon resonance (SPR). Variety of biomolecules were revealed by FTIR involved in reduction cum stabilization of zinc oxide nanoparticles...
June 13, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29896454/molecular-insights-into-development-and-virulence-determinants-of-aspergilli-a-proteomic-perspective
#13
REVIEW
Jata Shankar, Shraddha Tiwari, Sonia K Shishodia, Manali Gangwar, Shanu Hoda, Raman Thakur, Pooja Vijayaraghavan
Aspergillus species are the major cause of health concern worldwide in immunocompromised individuals. Opportunistic Aspergilli cause invasive to allergic aspergillosis, whereas non-infectious Aspergilli have contributed to understand the biology of eukaryotic organisms and serve as a model organism. Morphotypes of Aspergilli such as conidia or mycelia/hyphae helped them to survive in favorable or unfavorable environmental conditions. These morphotypes contribute to virulence, pathogenicity and invasion into hosts by excreting proteins, enzymes or toxins...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29896200/protein-deiminase-4-and-cr3-regulate-aspergillus-fumigatus-and-%C3%AE-glucan-induced-neutrophil-extracellular-trap-formation-but-hyphal-killing-is-dependent-only-on-cr3
#14
Heather L Clark, Serena Abbondante, Martin S Minns, Elyse N Greenberg, Yan Sun, Eric Pearlman
Neutrophil extracellular trap (NET) formation requires chromatin decondensation before nuclear swelling and eventual extracellular release of DNA, which occurs together with nuclear and cytoplasmic antimicrobial proteins. A key mediator of chromatin decondensation is protein deiminase 4 (PAD4), which catalyzes histone citrullination. In the current study, we examined the role of PAD4 and NETosis following activation of neutrophils by A. fumigatus hyphal extract or cell wall β-glucan (curdlan) and found that both induced NET release by human and murine neutrophils...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29894182/sterol-14%C3%AE-demethylase-structure-based-design-of-vni-r-n-1-2-4-dichlorophenyl-2-1h-imidazol-1-yl-ethyl-4-5-phenyl-1-3-4-oxadiazol-2-yl-benzamide-derivatives-to-target-fungal-infections-synthesis-biological-evaluation-and-crystallographic-analysis
#15
Laura Friggeri, Tatiana Y Hargrove, Zdzislaw Wawrzak, Anna L Blobaum, Girish Rachakonda, Craig W Lindsley, Fernando Villalta, W David Nes, Maurizio Botta, F Peter Guengerich, Galina I Lepesheva
Due to the increase in the number of immunocompromised patients, the incidence of invasive fungal infections is growing but the treatment efficiency remains unacceptably low. The most potent clinical systemic antifungals (azoles) are the derivatives of two scaffolds: ketoconazole and fluconazole. Being the safest antifungal drugs, they still have shortcomings, mainly because of pharmacokinetics and resistance. Here we report the successful use of the target fungal enzyme, sterol 14α-demethylase (CYP51), for structure-based design of novel antifungal drug candidates by minor modifications of VNI [(R)-N-(1-(2,4-dichlorophenyl)-2-(1H-imidazol-1-yl)ethyl)-4-(5-phenyl-1,3,4-oxadiazol-2-yl)benzamide)], an inhibitor of protozoan CYP51 that cures Chagas disease...
June 12, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29891611/identification-of-14-alpha-lanosterol-demethylase-cyp51-in-scedosporium-species
#16
Anne Bernhardt, Wieland Meyer, Volker Rickerts, Toni Aebischer, Kathrin Tintelnot
Scedosporium spp. cause infections (scedosporiosis) in both immunocompetent and immunocompromised individuals and may persistently colonise the respiratory tract in patients with cystic fibrosis (CF). They are less susceptible against azoles when compared with other moulds such as Aspergillus , suggesting the presence of resistance mechanisms. It can be hypothesised that the decreased susceptibility of Scedosporium spp. to azoles is also CYP51 dependant. Analysis of the S. apiospermum and S. aurantiacum genome revealed one CYP51 gene encoding the 14 alpha-lanosterol demethylase...
June 11, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29891608/molecular-confirmation-of-the-linkage-between-rhizopus-oryzae-cyp51a-gene-coding-region-and-its-intrinsic-voriconazole-and-fluconazole-resistance
#17
Daiana Macedo, Florencia Leonardelli, Catiana Dudiuk, Laura Theill, Matías S Cabeza, Soledad Gamarra, Guillermo Garcia-Effron
Rhizopus oryzae is the most prevalent causative agent of Mucormycosis, an increasingly reported opportunistic fungal infection. These mucormycetes are intrinsically resistant to Candida - and Aspergillus -active antifungal azole drugs such as fluconazole and voriconazole, respectively. Despite of its importance, the molecular mechanisms of its intrinsic azole resistance have not been elucidated yet. The aim of this work is to establish if the Rhizopus oryzae CYP51 genes are the unique responsible for voriconazole and fluconazole intrinsic resistance in these fungal pathogens...
June 11, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29891597/-emergence-of-azole-resistant-aspergillus-fumigatus-from-immunocompromised-hosts-in-india
#18
Yubhisha Dabas, Immaculata Xess, Sameer Bakshi, Manoranjan Mahapatra, Rachna Seth
This prospective study shows that the rate of azole resistant Aspergillus fumigatus (ARAF) in Indian immunocompromised patient population with invasive aspergillosis (IA) is low, 6/706 (0.8%). This lower rate supports the continued use of voriconazole as the first line of treatment. However, the ARAF in this study exhibited three kinds of unreported cyp51A mutations from India, of which two were at hot spots, G54R and P216L while one was at codon Y431C.
June 11, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29891595/the-effect-of-the-novel-antifungal-drug-f901318-olorofim-on-the-growth-and-viability-of-aspergillus-fumigatus
#19
Saskia du Pré, Nicola Beckmann, Mariana Cruz Almeida, Graham E M Sibley, Derek Law, Alexandra C Brand, Mike Birch, Nick D Read, Jason D Oliver
F901318 (olorofim) is a novel antifungal drug that is highly active against Aspergillus species. Belonging to a new class of antifungals called the orotomides, F901318 targets dihydroorotate dehydrogenase (DHODH) in the de novo pyrimidine biosynthesis pathway. In this study, the antifungal effects of F901318 against Aspergillus fumigatus were investigated. Live-cell imaging revealed that, at a concentration of 0.1 μg/ml, F901318 completely inhibited germination, but conidia continued to expand by isotropic growth for >120 h...
June 11, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29889264/transcriptional-profiling-of-scedosporium-apiospermum-enzymatic-antioxidant-gene-battery-unravels-the-involvement-of-thioredoxin-reductases-against-chemical-and-phagocytic-cells-oxidative-stress
#20
Cindy Staerck, Julie Tabiasco, Charlotte Godon, Yves Delneste, Jean-Philippe Bouchara, Maxime J J Fleury
Scedosporium species rank the second, after Aspergillus fumigatus, among the filamentous fungi colonizing the airways of patients with cystic fibrosis (CF). Development of microorganisms in the respiratory tract depends on their capacity to evade killing by the host immune system, particularly through the oxidative response of macrophages and neutrophils, with the release of reactive oxygen species (ROS) and reactive nitrogen species (RNS). This is particularly true in the airways of CF patients which display an exacerbated inflammatory reaction...
June 8, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
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