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https://www.readbyqxmd.com/read/29451601/cell-division-in-genus-corynebacterium-protein-protein-interaction-and-molecular-docking-of-sepf-and-ftsz-in-the-understanding-of-cytokinesis-in-pathogenic-species
#1
Alberto F Oliveira, Edson L Folador, Anne C P Gomide, Aristóteles Goes-Neto, Vasco A C Azevedo, Alice R Wattam
The genus Corynebacterium includes species of great importance in medical, veterinary and biotechnological fields. The genus-specific families (PLfams) from PATRIC have been used to observe conserved proteins associated to all species. Our results showed a large number of conserved proteins that are associated with the cellular division process. Was not observe in our results other proteins like FtsA and ZapA that interact with FtsZ. Our findings point that SepF overlaps the function of this proteins explored by molecular docking, protein-protein interaction and sequence analysis...
February 15, 2018: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29445369/turgor-pressure-and-possible-constriction-mechanisms-in-bacterial-division
#2
REVIEW
Masaki Osawa, Harold P Erickson
Bacterial cytokinesis begins with the assembly of FtsZ into a Z ring at the center of the cell. The Z-ring constriction in Gram-negative bacteria may occur in an environment where the periplasm and the cytoplasm are isoosmotic, but in Gram-positive bacteria the constriction may have to overcome a substantial turgor pressure. We address three potential sources of invagination force. (1) FtsZ itself may generate force by curved protofilaments bending the attached membrane. This is sufficient to constrict liposomes in vitro ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29443967/peptidoglycan-synthesis-drives-an-ftsz-treadmilling-independent-step-of-cytokinesis
#3
João M Monteiro, Ana R Pereira, Nathalie T Reichmann, Bruno M Saraiva, Pedro B Fernandes, Helena Veiga, Andreia C Tavares, Margarida Santos, Maria T Ferreira, Vânia Macário, Michael S VanNieuwenhze, Sérgio R Filipe, Mariana G Pinho
Peptidoglycan is the main component of the bacterial wall and protects cells from the mechanical stress that results from high intracellular turgor. Peptidoglycan biosynthesis is very similar in all bacteria; bacterial shapes are therefore mainly determined by the spatial and temporal regulation of peptidoglycan synthesis rather than by the chemical composition of peptidoglycan. The form of rod-shaped bacteria, such as Bacillus subtilis or Escherichia coli, is generated by the action of two peptidoglycan synthesis machineries that act at the septum and at the lateral wall in processes coordinated by the cytoskeletal proteins FtsZ and MreB, respectively...
February 14, 2018: Nature
https://www.readbyqxmd.com/read/29433923/substitution-of-terminal-amide-with-1h-1-2-3-triazole-identification-of-unexpected-class-of-potent-antibacterial-agents
#4
Fangchao Bi, Shengli Ji, Henrietta Venter, Jingru Liu, Susan J Semple, Shutao Ma
3-Methoxybenzamide (3-MBA) derivatives have been identified as novel class of potent antibacterial agents targeting the bacterial cell division protein FtsZ. As one of isosteres for the amide group, 1,2,3-triazole can mimic the topological and electronic features of the amide, which has gained increasing attention in drug discovery. Based on these considerations, we prepared a series of 1H-1,2,3-triazole-containing 3-MBA analogues via isosteric replacement of the terminal amide with triazole, which had increased antibacterial activity...
February 2, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29414773/minc-and-ftsz-mutant-analysis-provides-insight-into-minc-mind-mediated-z-ring-disassembly
#5
Kyung-Tae Park, Alex Dajkovic, Mark Wissel, Shishen Du, Joe Lutkenhaus
The Min system negatively regulates the position of the Z ring, which serves as a scaffold for the divisome that mediates bacterial cytokinesis. In Escherichia coli this system consists of MinC, which antagonizes assembly of the tubulin homologue FtsZ. MinC is recruited to the membrane by MinD and induced by MinE to oscillate between the cell poles. MinC is a dimer with each monomer consisting of functionally distinct MinCN and MinCC domains, both of which contact FtsZ. According to one model MinCC /MinD binding to the FtsZ tail positions MinCN at the junction of two GDP containing subunits in the filament, leading to filament breakage...
February 2, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29379012/antimicrobial-activity-of-apple-cider-vinegar-against-escherichia-coli-staphylococcus-aureus-and-candida-albicans-downregulating-cytokine-and-microbial-protein-expression
#6
Darshna Yagnik, Vlad Serafin, Ajit J Shah
The global escalation in antibiotic resistance cases means alternative antimicrobials are essential. The aim of this study was to investigate the antimicrobial capacity of apple cider vinegar (ACV) against E. coli, S. aureus and C. albicans. The minimum dilution of ACV required for growth inhibition varied for each microbial species. For C. albicans, a 1/2 ACV had the strongest effect, S. aureus, a 1/25 dilution ACV was required, whereas for E-coli cultures, a 1/50 ACV dilution was required (p < 0.05)...
January 29, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29378890/the-conserved-dna-binding-protein-whia-influences-chromosome-segregation-in-bacillus-subtilis
#7
Laura C Bohorquez, Katarina Surdova, Martijs J Jonker, Leendert W Hamoen
The DNA binding protein WhiA is conserved in Gram-positive bacteria, and is also present in the genetically simple, cell wall-lacking, mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates expression of key differentiation genes, including the cell division gene ftsZ essential for sporulation. For Bacillus subtilis it was shown that WhiA is essential when certain cell division genes are deleted...
January 29, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29369011/structural-insights-of-staphylococcus-aureus-ftsz-inhibitors-through-molecular-docking-3d-qsar-and-molecular-dynamics-simulations
#8
Srilata Ballu, Ramesh Itteboina, Sree Kanth Sivan, Vijjulatha Manga
Filamentous temperature-sensitive protein Z (FtsZ) is a protein encoded by the FtsZ gene that assembles into a Z-ring at the future site of the septum of bacterial cell division. Structurally, FtsZ is a homolog of eukaryotic tubulin but has low sequence similarity; this makes it possible to obtain FtsZ inhibitors without affecting the eukaryotic cell division. Computational studies were performed on a series of substituted 3-arylalkoxybenzamide derivatives reported as inhibitors of FtsZ activity in Staphylococcus aureus...
February 2018: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/29331569/salinivibrio-kushneri-sp-nov-a-moderately-halophilic-bacterium-isolated-from-salterns
#9
Clara López-Hermoso, Rafael R de la Haba, Cristina Sánchez-Porro, Antonio Ventosa
Ten Gram-strain-negative, facultatively anaerobic, moderately halophilic bacterial strains, designated AL184T, IB560, IB563, IC202, IC317, MA421, ML277, ML318, ML328A and ML331, were isolated from water ponds of five salterns located in Spain. The cells were motile, curved rods and oxidase and catalase positive. All of them grew optimally at 37°C, at pH 7.2-7.4 and in the presence of 7.5% (w/v) NaCl. Based on phylogenetic analyses of the 16S rRNA, the isolates were most closely related to Salinivibrio sharmensis BAGT (99...
December 21, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29313064/genetic-diversity-of-bartonella-spp-in-wild-mammals-and-ectoparasites-in-brazilian-pantanal
#10
Keyla Carstens Marques de Sousa, Renan Bressianini do Amaral, Heitor Miraglia Herrera, Filipe Martins Santos, Gabriel Carvalho Macedo, Pedro Cordeiro Estrela de Andrade Pinto, Darci Moraes Barros-Battesti, Rosangela Zacarias Machado, Marcos Rogério André
The present work aimed to investigate the genetic diversity of Bartonella in mammals and ectoparasites in Pantanal wetland, Brazil. For this purpose, 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 110 wild rodents, 30 marsupials, and 42 dogs were sampled. DNA samples were submitted to a quantitative real-time PCR assay (qPCR). Positive samples in qPCR were submitted to conventional PCR assays targeting other five protein-coding genes. Thirty-five wild rodents and three Polygenis (P.) bohlsi bohlsi flea pools showed positive results in qPCR for Bartonella spp...
January 8, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29311279/a-robust-crispri-gene-repression-system-in-pseudomonas
#11
Sue Zanne Tan, Christopher R Reisch, Kristala L J Prather
Pseudomonas spp. are widely used model organisms in different areas of research. Despite its relevance in many applications, protein depletion tools in this host remain limited. Here, we developed the CRISPR interference system for gene repression in Pseudomonas spp. using the Streptococcus pasteurianus dCas9. We demonstrate a robust and titratable gene depletion system with up to 100-fold repression in β-galactosidase activity in P. aeruginosa and 300-fold repression in pyoverdine production in P. putida This inducible system enables the study of essential genes, as shown by ftsZ depletions in P...
January 8, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29286204/genetic-variation-and-potential-co-infection-of-wolbachia-among-widespread-asian-citrus-psyllid-diaphorina-citri-kuwayama-populations
#12
Chia-Ching Chu, Mark Hoffmann, W Evan Braswell, Kirsten S Pelz-Stelinski
Wolbachia can profoundly influence the survival, reproduction, and defenses of insect hosts. These interactions could potentially be harnessed for managing pests or insect-transmitted diseases. Diaphorina citri Kuwayama is a phloem-feeding pest capable of transmitting the putative causal agent of citrus greening, Candidatus Liberibacter asiaticus (CLas). Like many insects, D. citri is also infected with Wolbachia (wDi). Recent studies indicate that the relative abundance of wDi could be associated with the abundance of CLas, and that wDi may contribute to regulating expression of phage lytic cycle genes in CLas, suggesting the need for better understanding of wDi biology in general...
December 29, 2017: Insect Science
https://www.readbyqxmd.com/read/29280220/ftsa-reshapes-membrane-architecture-and-remodels-the-z-ring-in-escherichia-coli
#13
Joseph Conti, Marissa G Viola, Jodi L Camberg
Cell division in prokaryotes initiates with assembly of the Z-ring at midcell, which, in Escherichia coli, is tethered to the inner leaflet of the cytoplasmic membrane through a direct interaction with FtsA, a widely conserved actin homolog. The Z-ring is comprised of polymers of tubulin-like FtsZ and has been suggested to provide the force for constriction. Here, we demonstrate that FtsA exerts force on membranes causing redistribution of membrane architecture, robustly hydrolyzes ATP and directly engages FtsZ polymers in a reconstituted system...
December 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29244730/isolation-and-characterization-of-ftsz-genes-in-cassava
#14
Meng-Ting Geng, Yi Min, Yuan Yao, Xia Chen, Jie Fan, Shuai Yuan, Lei Wang, Chong Sun, Fan Zhang, Lu Shang, Yun-Lin Wang, Rui-Mei Li, Shao-Ping Fu, Rui-Jun Duan, Jiao Liu, Xin-Wen Hu, Jian-Chun Guo
The filamenting temperature-sensitive Z proteins (FtsZs) play an important role in plastid division. In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ1 and FtsZ2). MeFtsZ1 with a putative signal peptide at N-terminal, has six exons, and is classed to FtsZ1 clade. MeFtsZ2-1 and MeFtsZ2-2 without a putative signal peptide, have seven exons, and are classed to FtsZ2 clade...
December 15, 2017: Genes
https://www.readbyqxmd.com/read/29237698/oxys-small-rna-induces-cell-cycle-arrest-to-allow-dna-damage-repair
#15
Shir Barshishat, Maya Elgrably-Weiss, Jonathan Edelstein, Jens Georg, Sutharsan Govindarajan, Meytal Haviv, Patrick R Wright, Wolfgang R Hess, Shoshy Altuvia
To maintain genome integrity, organisms employ DNA damage response, the underlying principles of which are conserved from bacteria to humans. The bacterial small RNA OxyS of Escherichia coli is induced upon oxidative stress and has been implicated in protecting cells from DNA damage; however, the mechanism by which OxyS confers genome stability remained unknown. Here, we revealed an OxyS-induced molecular checkpoint relay, leading to temporary cell cycle arrest to allow damage repair. By repressing the expression of the essential transcription termination factor nusG, OxyS enables read-through transcription into a cryptic prophage encoding kilR The KilR protein interferes with the function of the major cell division protein FtsZ, thus imposing growth arrest...
December 13, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29233778/phenotypic-indications-of-ftsz-inhibition-in-hok-sok-induced-bacterial-growth-changes-and-stress-response
#16
ChinweU Chukwudi, Liam Good
The hok/sok locus has been shown to enhance the growth of bacteria in adverse growth conditions such as high temperature, low starting-culture densities and antibiotic treatment. This is in addition to their well-established plasmid-stabilization effect via post-segregational killing of plasmid-free daughter cells. It delays the onset of growth by prolonging the lag phase of bacterial culture, and increases the rate of exponential growth when growth eventually begins. This enables the cells adapt to the prevailing growth conditions and enhance their survival in stressful conditions...
December 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29233095/the-distinctive-cell-division-interactome-of-neisseria-gonorrhoeae
#17
Yinan Zou, Yan Li, Jo-Anne R Dillon
BACKGROUND: Bacterial cell division is an essential process driven by the formation of a Z-ring structure, as a cytoskeletal scaffold at the mid-cell, followed by the recruitment of various proteins which form the divisome. The cell division interactome reflects the complement of different interactions between all divisome proteins. To date, only two cell division interactomes have been characterized, in Escherichia coli and in Streptococcus pneumoniae. The cell divison proteins encoded by Neisseria gonorrhoeae include FtsZ, FtsA, ZipA, FtsK, FtsQ, FtsI, FtsW, and FtsN...
December 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29209072/free-sepf-interferes-with-recruitment-of-late-cell-division-proteins
#18
Yongqiang Gao, Michaela Wenzel, Martijs J Jonker, Leendert W Hamoen
The conserved cell division protein SepF aligns polymers of FtsZ, the key cell division protein in bacteria, during synthesis of the (Fts)Z-ring at midcell, the first stage in cytokinesis. In addition, SepF acts as a membrane anchor for the Z-ring. Recently, it was shown that SepF overexpression in Mycobacterium smegmatis blocks cell division. Why this is the case is not known. Surprisingly, we found in Bacillus subtilis that SepF overproduction does not interfere with Z-ring assembly, but instead blocks assembly of late division proteins responsible for septum synthesis...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29202109/ylmd-and-ylme-genes-are-dispensable-for-growth-cross-wall-formation-and-sporulation-in-streptomyces-venezuelae
#19
Fernando Santos-Beneit, Jing-Ying Gu, Ulrich Stimming, Jeff Errington
Streptomycetes are Gram-positive filamentous soil bacteria that grow by tip extension and branching, forming a network of multinucleoid hyphae. These bacteria also have an elaborate process of morphological differentiation, which involves the formation of an aerial mycelium that eventually undergoes extensive septation into chains of uninucleoid cells that further metamorphose into spores. The tubulin-like FtsZ protein is essential for this septation process. Most of the conserved cell division genes (including ftsZ) have been inactivated in Streptomyces without the anticipated lethality, based on studies of many other bacteria...
November 2017: Heliyon
https://www.readbyqxmd.com/read/29193432/spatial-separation-of-ftsz-and-ftsn-during-cell-division
#20
Bill Söderström, Helena Chan, Patrick J Shilling, Ulf Skoglund, Daniel O Daley
The division of Escherichia coli is mediated by a collection of some 34 different proteins that are recruited to the division septum and are thought to assemble into a macromolecular complex known as 'the divisome'. Herein we have endeavored to better understand the structure of the divisome by imaging two of its core components; FtsZ and FtsN. Super resolution microscopy (SIM and gSTED) indicated that both proteins are localized in large assemblies, which are distributed around the division septum (i.e. forming a discontinuous ring)...
November 29, 2017: Molecular Microbiology
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