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https://www.readbyqxmd.com/read/27902442/the-whcd-gene-of-corynebacterium-glutamicum-plays-roles-in-cell-division-and-envelope-formation
#1
Dong-Seok Lee, Younhee Kim, Heung-Shick Lee
In this study, we analyzed the whcD gene from Corynebacterium glutamicum, which encodes a homolog of whiB, a Streptomyces coelicolor gene required for the sporulation of aerial hyphae. Deletion of the gene (ΔwhcD) severely affected cell growth in C. glutamicum. The ΔwhcD strain exhibited a large, filamentous, branched, and bud-shaped morphology with multiple septa. The transcription levels of the cell division genes involved in Z-ring assembly and septal peptidoglycan synthesis, including ftsZ, sepF, ftsQ, and ftsI, were markedly decreased in the ΔwhcD strain...
November 24, 2016: Microbiology
https://www.readbyqxmd.com/read/27902186/multigenetic-characterization-of-candidatus-xenohaliotis-californiensis
#2
Francesco Cicala, James D Moore, Jorge Cáceres-Martínez, Miguel A Del Río-Portilla, Mónica Hernández-Rodríguez, Rebeca Vásquez-Yeomans, Axayácatl Rocha-Olivares
"Candidatus Xenohaliotis californiensis" (or Ca. Xc) is the etiological agent of the withering syndrome (WS), a chronic wasting disease affecting most if not all North American species of abalone, and has been described as a Rickettsiales-like prokaryote. Genetic data about this species is limited to the 16S rRNA gene. The inability to grow it axenically has hindered its genetic and genomic characterization and, in consequence, a thorough analysis of its systematics. Here, we amplified and sequenced five genes (16S rRNA, 23S rRNA, ftsZ, virD4, and virB11) of Ca...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27891682/robust-min-system-oscillation-in-the-presence-of-internal-photosynthetic-membranes-in-cyanobacteria
#3
Joshua S MacCready, Jory Schossau, Katherine W Osteryoung, Daniel C Ducat
The oscillatory Min system of Escherichia coli defines the cell division plane by regulating the site of FtsZ-ring formation and represents one of the best-understood examples of emergent protein self-organization in nature. The oscillatory patterns of the Min-system proteins MinC, MinD and MinE (MinCDE) are strongly dependent on the geometry of membranes they bind. Complex internal membranes within cyanobacteria could disrupt this self-organization by sterically occluding or sequestering MinCDE from the plasma membrane...
November 5, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27876798/proliferation-of-listeria-monocytogenes-l-form-cells-by-formation-of-internal-and-external-vesicles
#4
Patrick Studer, Titu Staubli, Noémi Wieser, Patrick Wolf, Markus Schuppler, Martin J Loessner
L-forms are cell wall-deficient bacteria that divide through unusual mechanisms, involving dynamic perturbations of the cellular shape and generation of vesicles, independently of the cell-division protein FtsZ. Here we describe FtsZ-independent mechanisms, involving internal and external vesicles, by which Listeria monocytogenes L-forms proliferate. Using micromanipulation of single cells and vesicles, we show that small vesicles are formed by invagination within larger intracellular vesicles, receive cytoplasmic content, and represent viable progeny...
November 23, 2016: Nature Communications
https://www.readbyqxmd.com/read/27872183/roles-of-the-essential-protein-ftsa-in-cell-growth-and-division-in-streptococcus-pneumoniae
#5
Andrea Mura, Daniela Fadda, Amilcar J Perez, Madeline L Danforth, Daniela Musu, Ana Isabel Rico, Marcin Krupka, Dalia Denapaite, Ho-Ching T Tsui, Malcolm E Winkler, Pavel Branny, Miguel Vicente, William Margolin, Orietta Massidda
: Streptococcus pneumoniae is an ovoid-shaped Gram-positive bacterium that grows by carrying out peripheral and septal peptidoglycan (PG) synthesis, analogous to model bacilli such as Escherichia coli and Bacillus subtilis In the model bacilli, FtsZ and FtsA proteins assemble into a ring at midcell and are dedicated to septal PG synthesis, but not peripheral PG synthesis; hence inactivation of FtsZ or FtsA results in long filamentous cells unable to divide. Here we demonstrate that FtsA and FtsZ colocalize at midcell in S...
November 21, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27871791/whmd-promotes-the-assembly-of-mycobacterium-smegmatis-ftsz-a-possible-role-of-whmd-in-bacterial-cell-division
#6
Dipanwita Bhattacharya, Ashutosh Kumar, Dulal Panda
WhmD is considered to have a role in the septation and division of Mycobacterium smegmatis cells. Since FtsZ is the central protein of the septum, we determined the effect of WhmD on the assembly of Mycobacterium smegmatis FtsZ (MsFtsZ) in vitro. WhmD increased both the rate and extent of the assembly of MsFtsZ in vitro. WhmD also increased the amount of polymerized MsFtsZ as evident from a sedimentation assay. Further, the assembly promoting activity of WhmD occurred in the presence of GTP. MsFtsZ polymerized to form thin filaments in the absence of WhmD while MsFtsZ formed thick filaments in the presence of WhmD suggesting that WhmD enhanced the bundling of MsFtsZ filaments...
November 19, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27871169/structural-and-biochemical-studies-reveal-a-putative-ftsz-recognition-site-on-the-z-ring-stabilizer-zapd
#7
Hwajung Choi, Kyungjin Min, Bunzo Mikami, Hye-Jin Yoon, Hyung Ho Lee
FtsZ, a tubulin homologue, is an essential protein of the Z-ring assembly in bacterial cell division. It consists of two domains, the N-terminal and C-terminal core domains, and has a conserved C-terminal tail region. Lateral interactions between FtsZ protofilaments and several Z-ring associated proteins (Zaps) are necessary for modulating Z-ring formation. ZapD, one of the positive regulators of Z-ring assembly, directly binds to the C-terminal tail of FtsZ and promotes stable Z-ring formation during cytokinesis...
November 30, 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27822514/a-prophage-encoded-small-rna-controls-metabolism-and-cell-division-in-escherichia-coli
#8
Divya Balasubramanian, Preethi T Ragunathan, Jingyi Fei, Carin K Vanderpool
Hundreds of small RNAs (sRNAs) have been identified in diverse bacterial species, and while the functions of most remain unknown, some regulate key processes, particularly stress responses. The sRNA DicF was identified over 25 years ago as an inhibitor of cell division but since then has remained uncharacterized. DicF consists of 53 nucleotides and is encoded by a gene carried on a prophage (Qin) in the genomes of many Escherichia coli strains. We demonstrated that DicF inhibits cell division via direct base pairing with ftsZ mRNA to repress translation and prevent new synthesis of the bacterial tubulin homolog FtsZ...
January 2016: MSystems
https://www.readbyqxmd.com/read/27810886/comparative-analysis-of-the-survival-and-gene-expression-of-pathogenic-strains-vibrio-harveyi-after-starvation
#9
Jingjing Sun, Xiaojian Gao, Jiang Qun, Xuedi Du, Keran Bi, Xiaojun Zhang, Li Lin
This study aimed to evaluate the survival and gene expression of Vibrio harveyi under starvation conditions. The microcosms V. harveyi were incubated in sterilized seawater for four weeks at room temperature. Overall, the cell numeration declined rapidly about 10(3) CFU/ ml during starvation, with a tiny rebound at day twenty-one. Scanning electron microscopy revealed rod-shaped cells became sphere with a rippled cell surface. By polymerase chain reaction (PCR) assay, nine genes, named luxR, toxR, vhhB, flaA, topA, fur, rpoS, mreB, ftsZ were detected in the non-starvedi cells...
November 2, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27795325/probing-for-binding-regions-of-the-ftsz-protein-surface-through-site-directed-insertions-discovery-of-fully-functional-ftsz-fps
#10
Desmond A Moore, Zakiya N Whatley, Chandra P Joshi, Masaki Osawa, Harold P Erickson
: FtsZ, a bacterial tubulin homologue, is a cytoskeleton protein that assembles into protofilaments, one subunit thick. These protofilaments assemble further to form a "Z ring" at the center of prokaryotic cells. The Z ring generates a constriction force on the inner membrane, and also serves as a scaffold to recruit cell-wall remodeling proteins for complete cell division in vivo. One model of the Z ring proposes that protofilaments associate via lateral bonds to form ribbons; however, lateral bonds are still only hypothetical...
October 17, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27784279/developmental-transcriptome-of-resting-cell-formation-in-mycobacterium-smegmatis
#11
Mu-Lu Wu, Martin Gengenbacher, Jade C S Chung, Swaine Lin Chen, Hans-Joachim Mollenkopf, Stefan H E Kaufmann, Thomas Dick
BACKGROUND: Mycobacteria, along with exospore forming Streptomyces, belong to the phylum actinobacteria. Mycobacteria are generally believed to be non-differentiating. Recently however, we showed that the mycobacterial model organism M. smegmatis is capable of forming different types of morphologically distinct resting cells. When subjected to starvation conditions, cells of M. smegmatis exit from the canonical cell division cycle, segregate and compact their chromosomes, and become septated and multi-nucleoided...
October 26, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27768711/screening-and-development-of-new-inhibitors-of-ftsz-from-m-tuberculosis
#12
Bini Mathew, Judith Varady Hobrath, Larry Ross, Michele C Connelly, Hava Lofton, Malini Rajagopalan, R Kiplin Guy, Robert C Reynolds
A variety of commercial analogs and a newer series of Sulindac derivatives were screened for inhibition of M. tuberculosis (Mtb) in vitro and specifically as inhibitors of the essential mycobacterial tubulin homolog, FtsZ. Due to the ease of preparing diverse analogs and a favorable in vivo pharmacokinetic and toxicity profile of a representative analog, the Sulindac scaffold may be useful for further development against Mtb with respect to in vitro bacterial growth inhibition and selective activity for Mtb FtsZ versus mammalian tubulin...
2016: PloS One
https://www.readbyqxmd.com/read/27752253/cytological-profile-of-antibacterial-ftsz-inhibitors-and-synthetic-peptide-mciz
#13
Lidia Araújo-Bazán, Laura B Ruiz-Avila, David Andreu, Sonia Huecas, José M Andreu
Cell division protein FtsZ is the organizer of the cytokinetic ring in almost all bacteria and a target for the discovery of new antibacterial agents that are needed to counter widespread antibiotic resistance. Bacterial cytological profiling, using quantitative microscopy, is a powerful approach for identifying the mechanism of action of antibacterial molecules affecting different cellular pathways. We have determined the cytological profile on Bacillus subtilis cells of a selection of small molecule inhibitors targeting FtsZ on different binding sites...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27729511/life-without-division-physiology-of-escherichia-coli-ftsz-deprived-filaments
#14
Alicia Sánchez-Gorostiaga, Pilar Palacios, Rocío Martínez-Arteaga, Manuel Sánchez, Mercedes Casanova, Miguel Vicente
: When deprived of FtsZ, Escherichia coli cells (VIP205) grown in liquid form long nonseptated filaments due to their inability to assemble an FtsZ ring and their failure to recruit subsequent divisome components. These filaments fail to produce colonies on solid medium, in which synthesis of FtsZ is induced, upon being diluted by a factor greater than 4. However, once the initial FtsZ levels are recovered in liquid culture, they resume division, and their plating efficiency returns to normal...
October 11, 2016: MBio
https://www.readbyqxmd.com/read/27726004/deconstructing-the-chlamydial-cell-wall
#15
Anna Klöckner, Henrike Bühl, Patrick Viollier, Beate Henrichfreise
The evolutionary separated Gram-negative Chlamydiales show a biphasic life cycle and replicate exclusively within eukaryotic host cells. Members of the genus Chlamydia are responsible for many acute and chronic diseases in humans, and Chlamydia-related bacteria are emerging pathogens. We revisit past efforts to detect cell wall material in Chlamydia and Chlamydia-related bacteria in the context of recent breakthroughs in elucidating the underlying cellular and molecular mechanisms of the chlamydial cell wall biosynthesis...
September 21, 2016: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/27725777/microenvironments-created-by-liquid-liquid-phase-transition-control-the-dynamic-distribution-of-bacterial-division-ftsz-protein
#16
Begoña Monterroso, Silvia Zorrilla, Marta Sobrinos-Sanguino, Christine D Keating, Germán Rivas
The influence of membrane-free microcompartments resulting from crowding-induced liquid/liquid phase separation (LLPS) on the dynamic spatial organization of FtsZ, the main component of the bacterial division machinery, has been studied using several LLPS systems. The GTP-dependent assembly cycle of FtsZ is thought to be crucial for the formation of the septal ring, which is highly regulated in time and space. We found that FtsZ accumulates in one of the phases and/or at the interface, depending on the system composition and on the oligomerization state of the protein...
October 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27723729/asynchronous-division-by-non-ring-ftsz-in-the-gammaproteobacterial-symbiont-of-robbea-hypermnestra
#17
Nikolaus Leisch, Nika Pende, Philipp M Weber, Harald R Gruber-Vodicka, Jolanda Verheul, Norbert O E Vischer, Sophie S Abby, Benedikt Geier, Tanneke den Blaauwen, Silvia Bulgheresi
The reproduction mode of uncultivable microorganisms deserves investigation as it can largely diverge from conventional transverse binary fission. Here, we show that the rod-shaped gammaproteobacterium thriving on the surface of the Robbea hypermnestra nematode divides by FtsZ-based, non-synchronous invagination of its poles-that is, the host-attached and fimbriae-rich pole invaginates earlier than the distal one. We conclude that, in a naturally occurring animal symbiont, binary fission is host-oriented and does not require native FtsZ to polymerize into a ring at any septation stage...
October 10, 2016: Nature Microbiology
https://www.readbyqxmd.com/read/27703450/first-report-of-vibrio-tubiashii-associated-with-a-massive-larval-mortality-event-in-a-commercial-hatchery-of-scallop-argopecten-purpuratus-in-chile
#18
Rodrigo Rojas, Claudio D Miranda, Javier Santander, Jaime Romero
The VPAP30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the Chilean scallop Argopecten purpuratus. The main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods to demonstrate its pathogenic activity on scallop larvae, to characterize its pathogenic properties and to describe the chronology of this pathology. The pathogenic strain was identified as Vibrio tubiashii based on its phenotypic properties and the sequence analysis of its 16S rRNA and housekeeping genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27693319/crispri-engineering-e-coli-for-morphology-diversification
#19
Dina Elhadi, Li Lv, Xiao-Ran Jiang, Hong Wu, Guo-Qiang Chen
Microbial morphology engineering has recently become interesting for biotechnology. Genes ftsZ and mreB encoding proteins of bacterial fission ring and skeletons, respectively, are essential for cell growth, they both are the most important genes keeping the bacterial shapes including the cell length and width, respectively. Clustered regularly interspaced short palindromic repeats interference, abbreviated as CRISPRi, was for the first time used in this study to regulate expression intensities of ftsZ or/and mreB in E...
September 28, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27693041/vibrio-barjaei-sp-nov-a-new-species-of-the-mediterranei-clade-isolated-in-a-shellfish-hatchery
#20
Javier Dubert, Sabela Balboa, María Regueira, Adrián González-Castillo, Bruno Gómez-Gil, Jesús L Romalde
Seven isolates were obtained from different culture stages of carpet shell clam (Ruditapes decussatus) reared in a bivalve hatchery (Galicia, NW Spain). Three groups were differentiated by genotyping techniques and phenotypic profiles and representative trains were selected to further taxonomic studies. These strains were studied by a polyphasic approach and in basis of the phylogenetic analysis based on concatenated sequences of the five housekeeping genes ftsZ, gyrB, pyrH, recA and rpoA formed a tight group into the Mediterranei clade of the genus Vibrio...
December 2016: Systematic and Applied Microbiology
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