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https://www.readbyqxmd.com/read/28300142/late-assembly-of-the-vibrio-cholerae-cell-division-machinery-postpones-septation-to-the-last-10-of-the-cell-cycle
#1
Elisa Galli, Evelyne Paly, François-Xavier Barre
Bacterial cell division is a highly regulated process, which involves the formation of a complex apparatus, the divisome, by over a dozen proteins. In the few model bacteria in which the division process was detailed, divisome assembly occurs in two distinct steps: a few proteins, including the FtsZ tubulin-like protein, form a membrane associated contractile ring, the Z-ring, at ~30% of the cell cycle. The Z-ring serves as a scaffold for the recruitment of a second series of proteins, including integral membrane and periplasmic cell wall remodelling enzymes, at ~50% of the cell cycle...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28285910/synthesis-and-antibacterial-activity-of-3-benzylamide-derivatives-as-ftsz-inhibitors
#2
Zhongping Hu, Shasha Zhang, Weicheng Zhou, Xiang Ma, Guangya Xiang
The emergence and spread of multidrug-resistant strains of the human pathological bacteria are generating a threat to public health worldwide. In the current study, a series of PC190723 derivatives was synthesized and investigated for their antimicrobial activity. The compounds exhibited good activity against several Gram-positive bacteria as determined by comparison of diameters of the zone of inhibition of test compounds and standard antibiotics. Compound 9 with a fluorine substitution on the phenyl ring showed the best antibacterial activity in the series against M...
February 17, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28266315/physiology-and-pathogenicity-of-cpdb-deleted-mutant-of-avian-pathogenic-escherichia-coli
#3
Huifang Liu, Liping Chen, Wei Si, Chunlai Wang, Fangna Zhu, Guangxing Li, Siguo Liu
Avian colibacillosis is one of the most common infectious diseases caused partially or entirely by avian pathogenic Escherichia coli (APEC) in birds. In addition to spontaneous infection, APEC can also cause secondary infections that result in greater severity of illness and greater losses to the poultry industry. In order to assess the role of 2', 3'-cyclic phosphodiesterase (cpdB) in APEC on disease physiology and pathogenicity, an avian pathogenic Escherichia coli-34 (APEC-34) cpdB mutant was obtained using the Red system...
November 17, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/28258494/three-rings-for-the-evolution-of-plastid-shape-a-tale-of-land-plant-ftsz
#4
Christopher Grosche, Stefan A Rensing
Nuclear-encoded plant FtsZ genes are derived from endosymbiotic gene transfer of cyanobacteria-like genes. The green lineage (Chloroplastida) and red lineage (Rhodophyta) feature FtsZ1 and FtsZ2 or FtsZB and FtsZA, respectively, which are involved in plastid division. These two proteins show slight differences and seem to heteropolymerize to build the essential inner plastid division ring. A third gene, encoding FtsZ3, is present in glaucophyte and charophyte algae, as well as in land plants except ferns and angiosperms...
March 3, 2017: Protoplasma
https://www.readbyqxmd.com/read/28257056/interaction-of-type-iv-toxin-antitoxin-systems-in-cryptic-prophages-of-escherichia-coli-k-12
#5
Zhongling Wen, Pengxia Wang, Chenglong Sun, Yunxue Guo, Xiaoxue Wang
Toxin/antitoxin (TA) systems are widespread in prokaryotic chromosomes and in mobile genetic elements including plasmids and prophages. The first characterized Type IV TA system CbtA/CbeA was found in cryptic prophage CP4-44 in Escherichia coli K-12. Two homologous TA loci of CbtA/CbeA also reside in cryptic prophages of E. coli K-12, YkfI/YafW in CP4-6 and YpjF/YfjZ in CP4-57. In this study, we demonstrated that YkfI and YpjF inhibited cell growth and led to the formation of "lemon-shaped" cells. Prolonged overproduction of YkfI led to the formation of "gourd-shaped" cells and immediate cell lysis...
March 1, 2017: Toxins
https://www.readbyqxmd.com/read/28254403/the-divisome-at-25-the-road-ahead
#6
REVIEW
Tanneke den Blaauwen, Leendert W Hamoen, Petra Anne Levin
The identification of the FtsZ ring by Bi and Lutkenhaus in 1991 was a defining moment for the field of bacterial cell division. Not only did the presence of the FtsZ ring provide fodder for the next 25 years of research, the application of a then cutting-edge approach-immunogold labeling of bacterial cells-inspired other investigators to apply similarly state-of-the-art technologies in their own work. These efforts have led to important advances in our understanding of the factors underlying assembly and maintenance of the division machinery...
February 27, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28249098/zapa-and-zapb-form-an-ftsz-independent-structure-at-midcell
#7
Jackson A Buss, Nick T Peters, Jie Xiao, Thomas G Bernhardt
Cell division in Escherichia coli begins with the polymerization of FtsZ into a ring-like structure, the Z-ring, at midcell. All other division proteins are thought to require the Z-ring for recruitment to the future division site. Here, we report that the Z-ring associated proteins ZapA and ZapB form FtsZ-independent structures at midcell. Upon Z-ring disruption by the FtsZ polymerization antagonist SulA, ZapA remained at midcell as a cloud-like accumulation. Using ZapA(N60Y), a variant defective for interaction with FtsZ, it was established that these ZapA structures form without a connection to the Z-ring...
March 1, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28248291/structural-insights-into-the-coordination-of-plastid-division-by-the-arc6-pdv2-complex
#8
Wenhe Wang, Jinyu Li, Qingqing Sun, Xiaoyu Yu, Weiwei Zhang, Ning Jia, Chuanjing An, Yiqiong Li, Yanan Dong, Fengjiao Han, Ning Chang, Xiaomin Liu, Zhiling Zhu, You Yu, Shilong Fan, Maojun Yang, Shi-Zhong Luo, Hongbo Gao, Yue Feng
Chloroplasts divide by binary fission, which is accomplished by the simultaneous constriction of the FtsZ ring on the stromal side of the inner envelope membrane, and the ARC5 ring on the cytosolic side of the outer envelope membrane. The two rings are connected and coordinated mainly by the interaction between the inner envelope membrane protein ARC6 and the outer envelope membrane protein PDV2 in the intermembrane space. The underlying mechanism of this coordination is unclear to date. Here, we solved the crystal structure of the intermembrane space region of the ARC6-PDV2 complex...
March 1, 2017: Nature Plants
https://www.readbyqxmd.com/read/28235875/engineering-cyanobacterial-cell-morphology-for-enhanced-recovery-and-processing-of-biomass
#9
Adam Jordan, Jenna Chandler, Joshua S MacCready, Jingcheng Huang, Katherine W Osteryoung, Daniel C Ducat
Cyanobacteria are emerging as alternative crop species for the production of fuels, chemicals, and biomass. Yet, the success of these microbes depends upon the development of cost-effective technologies that permit scaled cultivation and cell harvesting. Here, we investigate the feasibility of engineering cell morphology in order to improve biomass recovery and decrease energetic costs associated with lysing cyanobacterial cells. Specifically, we modify the levels of Min system proteins in Synechococcus elongatus sp...
February 24, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28231272/vibrio-japonicus-sp-nov-a-novel-member-of-the-nereis-clade-in-the-genus-vibrio-isolated-from-the-coast-of-japan
#10
Hiroyasu Doi, Ikuko Osawa, Hayamitsu Adachi, Manabu Kawada
A novel Vibrio strain, JCM 31412T, was isolated from seawater collected from the Inland Sea (Setonaikai), Japan, and characterized as a Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, ovoid-shaped bacterium with one polar flagellum. Based on 16S rDNA gene identity, strain JCM 31412T showed a close relationship with type strains of Vibrio brasiliensis (LMG 20546T, 98.2% identity), V. harveyi (NBRC 15634T, 98.2%), V. caribbeanicus (ATCC BAA-2122T, 97.8%) and V. proteolyticus (NBRC 13287T, 97...
2017: PloS One
https://www.readbyqxmd.com/read/28209899/gtpase-activity-coupled-treadmilling-of-the-bacterial-tubulin-ftsz-organizes-septal-cell-wall-synthesis
#11
Xinxing Yang, Zhixin Lyu, Amanda Miguel, Ryan McQuillen, Kerwyn Casey Huang, Jie Xiao
The bacterial tubulin FtsZ is the central component of the cell division machinery, coordinating an ensemble of proteins involved in septal cell wall synthesis to ensure successful constriction. How cells achieve this coordination is unknown. We found that in Escherichia coli cells, FtsZ exhibits dynamic treadmilling predominantly determined by its guanosine triphosphatase activity. The treadmilling dynamics direct the processive movement of the septal cell wall synthesis machinery but do not limit the rate of septal synthesis...
February 17, 2017: Science
https://www.readbyqxmd.com/read/28209898/treadmilling-by-ftsz-filaments-drives-peptidoglycan-synthesis-and-bacterial-cell-division
#12
Alexandre W Bisson-Filho, Yen-Pang Hsu, Georgia R Squyres, Erkin Kuru, Fabai Wu, Calum Jukes, Yingjie Sun, Cees Dekker, Seamus Holden, Michael S VanNieuwenhze, Yves V Brun, Ethan C Garner
The mechanism by which bacteria divide is not well understood. Cell division is mediated by filaments of FtsZ and FtsA (FtsAZ) that recruit septal peptidoglycan-synthesizing enzymes to the division site. To understand how these components coordinate to divide cells, we visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis. We found that the division septum was built at discrete sites that moved around the division plane. FtsAZ filaments treadmilled circumferentially around the division ring and drove the motions of the peptidoglycan-synthesizing enzymes...
February 17, 2017: Science
https://www.readbyqxmd.com/read/28196701/filamenting-temperature-sensitive-mutant-z-inhibitors-from-glycyrrhiza-glabra-and-their-inhibitory-mode-of-action
#13
Takashi Matsui, Subehan Lallo, Khoirun Nisa, Hiroyuki Morita
FtsZ is an essential protein for bacterial cell division, and an attractive and underexploited novel antibacterial target protein. Screening of Indonesian plants revealed the inhibitory activity of the methanol extract of Glycyrrhiza glabra on the Bacillus subtilis FtsZ (BsFtsZ) GTPase, and further bioassay-guided fractionation of the active methanol extract led to the isolation of seven known polyketides (1-7). Among them, gancaonin I (1), glycyrin (3), and isolicoflavanol (5) exhibited anti-BsFtsZ GTPase activities, at levels comparable to that of the synthetic FtsZ inhibitor, Zantrin Z3...
February 3, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28174299/the-chloroplast-tubulin-homologs-ftsza-and-ftszb-from-the-red-alga-galdieria-sulphuraria-co-assemble-into-dynamic-filaments
#14
Yaodong Chen, Katie Porter, Masaki Osawa, Anne Marie Augustus, Sara L Milam, Chandra Joshi, Katherine W Osteryoung, Harold P Erickson
FtsZ is a homolog of eukaryotic tubulin and is present in almost all bacteria and many archaea, where it is the major cytoskeletal protein in the Z ring, required for cell division. Unlike some other cell organelles of prokaryotic origin, chloroplasts have retained FtsZ as an essential component of the division machinery. However, chloroplast FtsZs have been challenging to study because they are difficult to express and purify. To this end, we have used a FATT-tag expression system to produce as soluble proteins the two chloroplast FtsZs from Galdieria sulphuraria, a thermophilic red alga...
February 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28168121/effects-of-rhodomyrtone-on-gram-positive-bacterial-tubulin-homologue-ftsz
#15
Dennapa Saeloh, Michaela Wenzel, Thanyada Rungrotmongkol, Leendert Willem Hamoen, Varomyalin Tipmanee, Supayang Piyawan Voravuthikunchai
Rhodomyrtone, a natural antimicrobial compound, displays potent activity against many Gram-positive pathogenic bacteria, comparable to last-defence antibiotics including vancomycin and daptomycin. Our previous studies pointed towards effects of rhodomyrtone on the bacterial membrane and cell wall. In addition, a recent molecular docking study suggested that the compound could competitively bind to the main bacterial cell division protein FtsZ. In this study, we applied a computational approach (in silico), in vitro, and in vivo experiments to investigate molecular interactions of rhodomyrtone with FtsZ...
2017: PeerJ
https://www.readbyqxmd.com/read/28161514/biosorption-behavior-and-proteomic-analysis-of-escherichia-coli-p4-under-cadmium-stress
#16
Zaman Khan, Abdul Rehman, Muhammad Atif Nisar, Saima Zafar, Inga Zerr
Bacteria develop a variety of adaptations at transcriptomic, metabolomic and proteomic levels in order to survive potentially damaging environmental perturbations. Present study is exploring the fluctuations in proteome of E. coli P4 to knob Cd(+2)-induced cytotoxicity. An attempt was also made to integrate all these approaches to gain comprehensive insight of Cd(+2) stress response in E. coli P4. This study is exposing the altered behavior of various proteins and their underlying metabolic pathways which have previously not been reported with reference to Cd(+2) stress such as sulfoquinovose biosynthesis and degradation pathway...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28137947/the-discovery-of-the-prokaryotic-cytoskeleton-25th-anniversary
#17
Harold P Erickson
The year 2017 marks the 25th anniversary of the discovery of homologues of tubulin and actin in prokaryotes. Before 1992, it was largely accepted that tubulin and actin were unique to eukaryotes. Then three laboratories independently discovered that FtsZ, a protein already known as a key player in bacterial cytokinesis, had the "tubulin signature sequence" present in all α-, β-, and γ-tubulins. That same year, three candidates for bacterial actins were discovered in silico. X-ray crystal structures have since confirmed multiple bacterial proteins to be homologues of eukaryotic tubulin and actin...
February 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28114338/proteolysis-dependent-remodeling-of-the-tubulin-homolog-ftsz-at-the-division-septum-in-escherichia-coli
#18
Marissa G Viola, Christopher J LaBreck, Joseph Conti, Jodi L Camberg
During bacterial cell division a dynamic protein structure called the Z-ring assembles at the septum. The major protein in the Z-ring in Escherichia coli is FtsZ, a tubulin homolog that polymerizes with GTP. FtsZ is degraded by the two-component ATP-dependent protease ClpXP. Two regions of FtsZ, located outside of the polymerization domain in the unstructured linker and at the C-terminus, are important for specific recognition and degradation by ClpXP. We engineered a synthetic substrate containing green fluorescent protein (Gfp) fused to an extended FtsZ C-terminal tail (residues 317-383), including the unstructured linker and the C-terminal conserved region, but not the polymerization domain, and showed that it is sufficient to target a non-native substrate for degradation in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28100778/structure-of-the-z-ring-associated-protein-zapd-bound-to-the-c-terminal-domain-of-the-tubulin-like-protein-ftsz-suggests-mechanism-of-z-ring-stabilization-through-ftsz-crosslinking
#19
Maria Schumacher, Kuo-Hsiang Huang, Wenjie Zeng, Anuradha Janakiraman
Cell division in most bacteria is mediated by the tubulin-like FtsZ protein, which polymerizes in a GTP-dependent manner to form the cytokinetic Z ring. A diverse repertoire of FtsZ binding proteins affect FtsZ localization and polymerization to ensure correct Z ring formation. Many of these proteins bind the C-terminal domain (CTD) of FtsZ, which serves as a hub for FtsZ regulation. FtsZ ring-associated proteins, ZapA-D (Zaps), are important FtsZ regulatory proteins that stabilize FtsZ assembly and enhance Z ring formation by increasing lateral assembly of FtsZ protofilaments, which then form the Z ring...
January 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28095503/detection-of-a-potential-new-bartonella-species-candidatus-bartonella-rondoniensis-in-human-biting-kissing-bugs-reduviidae-triatominae
#20
Maureen Laroche, Jean-Michel Berenger, Oleg Mediannikov, Didier Raoult, Philippe Parola
BACKGROUND: Among the Reduviidae family, triatomines are giant blood-sucking bugs. They are well known in Central and South America where they transmit Trypanosoma cruzi to mammals, including humans, through their feces. This parasitic protozoan is the causative agent of Chagas disease, a major public health issue in endemic areas. Because of the medical and economic impact of Chagas disease, the presence of other arthropod-borne pathogens in triatomines was rarely investigated. METHODOLOGY/PRINCIPAL FINDINGS: In this study, seven triatomines species involved in the transmission of T...
January 2017: PLoS Neglected Tropical Diseases
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