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https://www.readbyqxmd.com/read/28638803/peptide-nucleic-acid-knockdown-and-intra-host-cell-complementation-of-ehrlichia-type-iv-secretion-system-effector
#1
Pratibha Sharma, Omid Teymournejad, Yasuko Rikihisa
Survival of Ehrlichia chaffeensis depends on obligatory intracellular infection. One of the barriers to E. chaffeensis research progress has been the inability, using conventional techniques, to generate knock-out mutants for genes essential for intracellular infection. This study examined the use of Peptide Nucleic Acids (PNAs) technology to interrupt type IV secretion system (T4SS) effector protein expression in E. chaffeensis followed by intracellular complementation of the effector to determine its requirement for infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28611224/random-subsets-of-structured-deterministic-frames-have-manova-spectra
#2
Marina Haikin, Ram Zamir, Matan Gavish
We draw a random subset of [Formula: see text] rows from a frame with [Formula: see text] rows (vectors) and [Formula: see text] columns (dimensions), where [Formula: see text] and [Formula: see text] are proportional to [Formula: see text] For a variety of important deterministic equiangular tight frames (ETFs) and tight non-ETFs, we consider the distribution of singular values of the [Formula: see text]-subset matrix. We observe that, for large [Formula: see text], they can be precisely described by a known probability distribution-Wachter's MANOVA (multivariate ANOVA) spectral distribution, a phenomenon that was previously known only for two types of random frames...
June 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28592294/exercise-in-claudicants-increase-or-decrease-walking-ability-and-the-response-relates-to-mitochondrial-function
#3
Michel van Schaardenburgh, Martin Wohlwend, Øivind Rognmo, Erney J R Mattsson
BACKGROUND: Exercise of patients with intermittent claudication improves walking performance. Exercise does not usually increase blood flow, but seems to increase muscle mitochondrial enzyme activities. Although exercise is beneficial in most patients, it might be harmful in some. The mitochondrial response to exercise might therefore differ between patients. Our hypothesis was that changes in walking performance relate to changes in mitochondrial function after 8 weeks of exercise. At a subgroup level, negative responders decrease and positive responders increase mitochondrial capacity...
June 7, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28417225/comparative-proteomic-analysis-provides-insight-into-10-hydroxy-2-decenoic-acid-biosynthesis-in-honey-bee-workers
#4
Xiao-Hui Yang, Shi-Fa Yang, Rui-Ming Wang
10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis was performed in the MGs of workers with different 10-HDA production. In total, 59 up-regulated protein species representing 45 unique proteins were identified in high 10-HDA-producing workers by 2-DE-MALDI-TOF/TOF MS...
April 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28362573/ultrasound-assessed-gastric-antral-area-correlates-with-aspirated-tube-feed-volume-in-enterally-fed-critically-ill-patients
#5
Vibhu Sharma, Deepthi Gudivada, Renaud Gueret, John Bailitz
BACKGROUND: Enteral tube feed (ETF) intolerance occurs frequently in hospitalized patients and more so in critically ill patients. Most critical care nurses continue to assess gastric residual volume (GRV), especially among those with a history of ETF intolerance. We hypothesized that ultrasound assessment of GRV correlates directly with aspirated tube feed volume. METHODS: This was a prospective cohort study of a convenience sample of critically ill mechanically ventilated patients admitted to an intensive care unit receiving ETF...
April 2017: Nutrition in Clinical Practice
https://www.readbyqxmd.com/read/28222553/dual-afferent-sensory-input-training-for-voluntary-movement-after-stroke-a-pilot-randomized-controlled-study
#6
Seahyun Bae, Kyung-Yoon Kim
BACKGROUND: Stimulation through afferent sensory input is necessary to improve voluntary functional movement in stroke patients. OBJECTIVES: Dual-afferent sensory input, which combines electromyography-triggered functional electric stimulation (ETFES) and action observation, was investigated to determine its effects on voluntary movements in stroke patients. METHODS: This study was conducted on 18 patients with left hemiplegia diagnosed between 6 and 24 months prior...
2017: NeuroRehabilitation
https://www.readbyqxmd.com/read/27932700/predictors-of-enteral-tube-feeding-in-hospitalized-older-adults
#7
Milton Roberto Furst Crenitte, Thiago Junqueira Avelino-Silva, Daniel Apolinario, Jose Antonio Esper Curiati, Flavia Campora, Wilson Jacob-Filho
BACKGROUND: Despite general recognition that enteral tube feeding (ETF) is frequently employed in long-term care facilities and patients with dementia, remarkably little research has determined which factors are associated with its use in acutely ill older adults. In this study, we aimed to investigate determinants of ETF introduction in hospitalized older adults. METHODS: We examined a retrospective cohort of acutely ill patients, aged 60 years and older, admitted to a university hospital's geriatric ward from 2014-2015, in São Paulo, Brazil...
December 8, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27916500/achieving-ethanol-type-fermentation-for-hydrogen-production-in-a-granular-sludge-system-by-aeration
#8
Song Zhang, Min Liu, Ying Chen, Yu-Ting Pan
To investigate the effects of aeration on hydrogen-producing granular system, experiments were performed in two laboratory-scale anaerobic internal circulation hydrogen production (AICHP) reactors. The preliminary experiment of Reactor 1 showed that direct aeration was beneficial to enhancing hydrogen production. After the direct aeration was implied in Reactor 2, hydrogen production rate (HPR) and hydrogen content were increased by 100% and 60%, respectively. In addition, mixed-acid fermentation was transformed into typical ethanol-type fermentation (ETF)...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27771746/identification-and-functional-analysis-of-the-gtpv-bidirectional-promoter-region
#9
Hui Zhang, Zhihua Sun, Na Zhang, Zhiqiang Li, Pengyan Wang, Qiang Fu, Yan Ren, Xuehua Shao, Yu Zhang, Zhiru Guo, Chuangfu Chen
The goat pox chick embryo-attenuated virus (GTPV) has been developed as an effective vaccine that can elicit protective immune responses. It possesses a large genome and a robust ability to express exogenous genes. Thus, this virus is an ideal vector for recombinant live vaccines for infectious diseases in ruminant animals. In this study, we identified a novel bidirectional promoter region of GTPV through screening named PbVV(±). PbVV(±) is located between ETF-l and VITF-3, which are transcribed in opposite directions...
March 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/27701823/differential-proteomic-analysis-of-the-metabolic-network-of-the-marine-sulfate-reducer-desulfobacterium-autotrophicum-hrm2
#10
Marvin Dörries, Lars Wöhlbrand, Ralf Rabus
The marine sulfate-reducing bacterium Desulfobacterium autotrophicum HRM2 belongs to the deltaproteobacterial family Desulfobacteraceae and stands out for its capacity of facultative chemolithoautotrophic growth (next to heterotrophy). Here, proteomics-driven metabolic reconstruction was based on a combination of 2D DIGE, shotgun proteomics and analysis of the membrane protein-enriched fraction applied to 8 different substrate adaptation conditions (7 aliphatic compounds plus H2 /CO2 ). In total 1,344 different proteins were identified (∼27% of the 4,947 genome-predicted) from which a complex metabolic network was reconstructed consisting of 136 proteins (124 detected; ∼91%)...
October 4, 2016: Proteomics
https://www.readbyqxmd.com/read/27673344/electron-beam-lithographic-grafting-of-functional-polymer-structures-from-fluoropolymer-substrates
#11
Katarzyna Gajos, Vitaliy A Guzenko, Matthias Dübner, Jakub Haberko, Andrzej Budkowski, Celestino Padeste
Well-defined submicrometer structures of poly(dimethylaminoethyl methacrylate) (PDMAEMA) were grafted from 100 μm thick films of poly(ethene-alt-tetrafluoroethene) after electron-beam lithographic exposure. To explore the possibilities and limits of the method under different exposure conditions, two different acceleration voltages (2.5 and 100 keV) were employed. First, the influence of electron energy and dose on the extent of grafting and on the structure's morphology was determined via atomic force microscopy...
October 7, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27541856/ehrlichia-secretes-etf-1-to-induce-autophagy-and-capture-nutrients-for-its-growth-through-rab5-and-class-iii-phosphatidylinositol-3-kinase
#12
Mingqun Lin, Hongyan Liu, Qingming Xiong, Hua Niu, Zhihui Cheng, Akitsugu Yamamoto, Yasuko Rikihisa
Ehrlichia chaffeensis is an obligatory intracellular bacterium that causes a potentially fatal emerging zoonosis, human monocytic ehrlichiosis. E. chaffeensis has a limited capacity for biosynthesis and metabolism and thus depends mostly on host-synthesized nutrients for growth. Although the host cell cytoplasm is rich with these nutrients, as E. chaffeensis is confined within the early endosome-like membrane-bound compartment, only host nutrients that enter the compartment can be used by this bacterium. How this occurs is unknown...
November 2016: Autophagy
https://www.readbyqxmd.com/read/27431485/comparative-genomics-of-syntrophic-branched-chain-fatty-acid-degrading-bacteria
#13
COMPARATIVE STUDY
Takashi Narihiro, Masaru K Nobu, Hideyuki Tamaki, Yoichi Kamagata, Yuji Sekiguchi, Wen-Tso Liu
The syntrophic degradation of branched-chain fatty acids (BCFAs) such as 2-methylbutyrate and isobutyrate is an essential step in the production of methane from proteins/amino acids in anaerobic ecosystems. While a few syntrophic BCFA-degrading bacteria have been isolated, their metabolic pathways in BCFA and short-chain fatty acid (SCFA) degradation as well as energy conservation systems remain unclear. In an attempt to identify these pathways, we herein performed comparative genomics of three syntrophic bacteria: 2-methylbutyrate-degrading "Syntrophomonas wolfei subsp...
September 29, 2016: Microbes and Environments
https://www.readbyqxmd.com/read/27427709/morphological-study-of-poly-vinylbenzyl-chloride-grafted-poly-ethylene-co-tetrafluoroethylene-etfe-g-pvbc-films-using-small-angle-neutron-scattering-analysis
#14
J M Song, B S Ko, J Y Sohn, J Shin
In this study, the effect of degree of the grafting and crosslinking on the morphology of the crystalline domain in poly(vinylbenzyl chloride)-grafted poly(ethylene-co-tetrafluoroethylene) [ETFE-g-PVBC] films was investigated using a SANS (small-angle neutron scattering) analysis. The grafted films can be used as a precursor for ion-exchange membrane. ETFE-g-PVBC films with various degrees of cross-linking were prepared by a simultaneous irradiation grafting of vinylbenzyl chloride (VBC) and divinylbenzene (DVB) onto an ETFE film...
June 2016: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/27367048/phenotypic-characterization-and-genetic-dissection-of-growth-period-traits-in-soybean-glycine-max-using-association-mapping
#15
Zhangxiong Liu, Huihui Li, Xuhong Fan, Wen Huang, Jiyu Yang, Candong Li, Zixiang Wen, Yinghui Li, Rongxia Guan, Yong Guo, Ruzhen Chang, Dechun Wang, Shuming Wang, Li-Juan Qiu
The growth period traits are important traits that affect soybean yield. The insights into the genetic basis of growth period traits can provide theoretical basis for cultivated area division, rational distribution, and molecular breeding for soybean varieties. In this study, genome-wide association analysis (GWAS) was exploited to detect the quantitative trait loci (QTL) for number of days to flowering (ETF), number of days from flowering to maturity (FTM), and number of days to maturity (ETM) using 4032 single nucleotide polymorphism (SNP) markers with 146 cultivars mainly from Northeast China...
2016: PloS One
https://www.readbyqxmd.com/read/27339419/gene-network-activity-in-cultivated-primary-hepatocytes-is-highly-similar-to-diseased-mammalian-liver-tissue
#16
Patricio Godoy, Agata Widera, Wolfgang Schmidt-Heck, Gisela Campos, Christoph Meyer, Cristina Cadenas, Raymond Reif, Regina Stöber, Seddik Hammad, Larissa Pütter, Kathrin Gianmoena, Rosemarie Marchan, Ahmed Ghallab, Karolina Edlund, Andreas Nüssler, Wolfgang E Thasler, Georg Damm, Daniel Seehofer, Thomas S Weiss, Olaf Dirsch, Uta Dahmen, Rolf Gebhardt, Umesh Chaudhari, Kesavan Meganathan, Agapios Sachinidis, Jens Kelm, Ute Hofmann, René P Zahedi, Reinhard Guthke, Nils Blüthgen, Steven Dooley, Jan G Hengstler
It is well known that isolation and cultivation of primary hepatocytes cause major gene expression alterations. In the present genome-wide, time-resolved study of cultivated human and mouse hepatocytes, we made the observation that expression changes in culture strongly resemble alterations in liver diseases. Hepatocytes of both species were cultivated in collagen sandwich and in monolayer conditions. Genome-wide data were also obtained from human NAFLD, cirrhosis, HCC and hepatitis B virus-infected tissue as well as mouse livers after partial hepatectomy, CCl4 intoxication, obesity, HCC and LPS...
October 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27232886/stability-and-degradation-mechanisms-of-radiation-grafted-polymer-electrolyte-membranes-for-water-electrolysis
#17
Albert Albert, Tim Lochner, Thomas J Schmidt, L Gubler
Radiation-grafted membranes are a promising alternative to commercial membranes for water electrolyzers, since they exhibit lower hydrogen crossover and area resistance, better mechanical properties, and are of potentially lower cost than perfluoroalkylsulfonic acid membranes, such as Nafion. Stability is an important factor in view of the expected lifetime of 40 000 h or more of an electrolyzer. In this study, combinations of styrene (St), α-methylstyrene (AMS), acrylonitrile (AN), and 1,3-diisopropenylbenzene (DiPB) are cografted into 50 μm preirradiated poly(ethylene-co-tetrafluoroethylene) (ETFE) base film, followed by sulfonation to produce radiation-grafted membranes...
June 22, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27206643/the-fabrication-of-nanoimprinted-p3ht-nanograting-by-patterned-etfe-mold-at-room-temperature-and-its-application-for-solar-cell
#18
Guangzhu Ding, Kaixuan Wang, Xiaohui Li, Qing Chen, Zhijun Hu, Jieping Liu
Nanoimprinting lithography (NIL) is investigated as a promising method to define nanostructure; however, finding a practical method to achieve large area patterning of conjugated polymer remains a challenge. We demonstrate here that a simple and cost-effective technique is proposed to fabricate the nanoimprinted P3HT nanograting by solvent-assisted room temperature NIL (SART-NIL) method with patterned ETFE film as mold. The patterned ETFE template is produced by embossing ETFE film into a patterned silicon master and is used as template to transfer nanogratings during the SART-NIL process...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27198766/methanogenic-paraffin-degradation-proceeds-via-alkane-addition-to-fumarate-by-smithella-spp-mediated-by-a-syntrophic-coupling-with-hydrogenotrophic-methanogens
#19
Boris Wawrik, Christopher R Marks, Irene A Davidova, Michael J McInerney, Shane Pruitt, Kathleen E Duncan, Joseph M Suflita, Amy V Callaghan
Anaerobic microbial biodegradation of recalcitrant, water-insoluble substrates, such as paraffins, presents unique metabolic challenges. To elucidate this process, a methanogenic consortium capable of mineralizing long-chain n-paraffins (C28 -C50 ) was enriched from San Diego Bay sediment. Analysis of 16S rRNA genes indicated the dominance of Syntrophobacterales (43%) and Methanomicrobiales (26%). Metagenomic sequencing allowed draft genome assembly of dominant uncultivated community members belonging to the bacterial genus Smithella and the archaeal genera Methanoculleus and Methanosaeta...
September 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27136103/superamphiphobic-and-electroactive-nanocomposite-toward-self-cleaning-antiwear-and-anticorrosion-coatings
#20
Ruixia Yuan, Shiqi Wu, Peng Yu, Baohui Wang, Liwen Mu, Xiguang Zhang, Yixing Zhu, Bing Wang, Huaiyuan Wang, Jiahua Zhu
Multifunctional coatings are in urgent demand in emerging fields. In this work, nanocomposite coatings with extraordinary self-cleaning, antiwear, and anticorrosion properties were prepared on aluminum substrate by a facile spraying technique. Core-shell structured polyaniline/functionalized carbon nanotubes (PANI/fCNTs) composite and nanosized silica were synergistically integrated into ethylene tetrafluoroethylene (ETFE) matrix to construct lotus-leaf-like structures, and 1H,1H,2H,2H- perfluorooctyltriethoxysilane (POTS) was used to decrease the surface energy...
May 18, 2016: ACS Applied Materials & Interfaces
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