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https://www.readbyqxmd.com/read/29738428/droplet-velocity-measurement-based-on-dielectric-layer-thickness-variation-using-digital-microfluidic-devices
#1
Siti Noor Idora Syafinaz Zulkepli, Nor Hisham Hamid, Vineeta Shukla
In recent years, the number of interdisciplinary research works related to the development of miniaturized systems with integrated chemical and biological analyses is increasing. Digital microfluidic biochips (DMFBs) are one kind of miniaturized systems designed for conducting inexpensive, fast, convenient and reliable biochemical assay procedures focusing on basic scientific research and medical diagnostics. The role of a dielectric layer in the digital microfluidic biochips is prominent as it helps in actuating microliter droplets based on the electrowetting-on-dielectric (EWOD) technique...
May 8, 2018: Biosensors
https://www.readbyqxmd.com/read/29645093/autologous-transplantation-versus-allogeneic-transplantation-in-patients-with-follicular-lymphoma-experiencing-early-treatment-failure
#2
Sonali M Smith, James Godfrey, Kwang Woo Ahn, Alyssa DiGilio, Sairah Ahmed, Vaibhav Agrawal, Veronika Bachanova, Ulrike Bacher, Asad Bashey, Javier Bolaños-Meade, Mitchell Cairo, Andy Chen, Saurabh Chhabra, Edward Copelan, Parastoo B Dahi, Mahmoud Aljurf, Umar Farooq, Siddhartha Ganguly, Mark Hertzberg, Leona Holmberg, David Inwards, Abraham S Kanate, Reem Karmali, Vaishalee P Kenkre, Mohamed A Kharfan-Dabaja, Andreas Klein, Hillard M Lazarus, Matthew Mei, Alberto Mussetti, Taiga Nishihori, Praveen Ramakrishnan Geethakumari, Ayman Saad, Bipin N Savani, Harry C Schouten, Nirav Shah, Alvaro Urbano-Ispizua, Ravi Vij, Julie Vose, Anna Sureda, Mehdi Hamadani
BACKGROUND: Early treatment failure (ETF) in follicular lymphoma (FL), defined as relapse or progression within 2 years of frontline chemoimmunotherapy, is a newly recognized marker of poor survival and identifies a high-risk group of patients with an expected 5-year overall survival (OS) rate of approximately 50%. Transplantation is an established option for relapsed FL, but its efficacy in this specific ETF FL population has not been previously evaluated. METHODS: This study compared autologous hematopoietic stem cell transplantation (auto-HCT) with either matched sibling donor (MSD) or matched unrelated donor (MUD) allogeneic hematopoietic cell transplantation (allo-HCT) as the first transplantation approach for patients with ETF FL (age ≥ 18 years) undergoing auto-HCT or allo-HCT between 2002 and 2014...
April 12, 2018: Cancer
https://www.readbyqxmd.com/read/29576319/structure-function-relationship-of-the-bik1-bim1-complex
#3
Marcel M Stangier, Anil Kumar, Xiuzhen Chen, Ana-Maria Farcas, Yves Barral, Michel O Steinmetz
In budding yeast, the microtubule plus-end tracking proteins Bik1 (CLIP-170) and Bim1 (EB1) form a complex that interacts with partners involved in spindle positioning, including Stu2 and Kar9. Here, we show that the CAP-Gly and coiled-coil domains of Bik1 interact with the C-terminal ETF peptide of Bim1 and the C-terminal tail region of Stu2, respectively. The crystal structures of the CAP-Gly domain of Bik1 (Bik1CG) alone and in complex with an ETF peptide revealed unique, functionally relevant CAP-Gly elements, establishing Bik1CG as a specific C-terminal phenylalanine recognition domain...
March 20, 2018: Structure
https://www.readbyqxmd.com/read/29566543/beetroot-based-gel-supplementation-improves-handgrip-strength-forearm-muscle-o2-saturation-but-not-exercise-tolerance-and-blood-volume-in-jiu-jitsu-athletes
#4
Gustavo Vieira de Oliveira, Luiz Nascimento, Mônica Volino-Souza, Jacilene Mesquita, Thiago Alvares
The ergogenic effect of beetroot on the exercise performance of trained cyclists, runners, kayakers, and swimmers has been demonstrated. However, whether or not beetroot supplementation presents a beneficial effect on the exercise performance of jiu-jitsu athletes (JJA) remains inconclusive. Therefore, present study assessed the effect of beetroot-based gel (BG) supplementation on maximal voluntary contraction (MVC), exercise time until fatigue (ETF), muscle O2 saturation (SmO2), blood volume (tHb), and plasma nitrate and lactate in response to handgrip isotonic exercise (HIE) in JJA...
March 22, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29515926/the-effect-of-nasal-functions-on-the-integrity-of-grafts-after-myringoplasty
#5
Başak Çaypınar Eser, Aslı Şahin Yılmaz, Serap Şahin Önder, Sema Zer Toros, Çağatay Oysu
Objective: We aimed to evaluate the effects of nasal functions for the integrity of grafts after myringoplasty. Methods: In our study 78 patients who underwent myringoplasty operation between 2011-2013 were included. Group I was defined as the group with an intact tympanic membrane following surgery. Group II was defined as the group with a tympanic membrane perforation following surgery. Group I consisted of 44 and Group II consisted of 34 patients. Subjective and objective measurements of nasal functions, Eustachian tube function (ETF), and allergic status were performed using nasal obstruction symptom evaluation (NOSE) scale, visual analog scale (VAS), and the score for allergic rhinitis (SFAR) questionnaires and acoustic rhinometry and saccharin test...
December 2017: Turkish Archives of Otorhinolaryngology
https://www.readbyqxmd.com/read/29462786/distinct-properties-underlie-flavin-based-electron-bifurcation-in-a-novel-electron-transfer-flavoprotein-fixab-from-rhodopseudomonas-palustris
#6
H Diessel Duan, Carolyn E Lubner, Monika Tokmina-Lukaszewska, George H Gauss, Brian Bothner, Paul W King, John W Peters, Anne-Frances Miller
A newly recognized third fundamental mechanism of energy conservation in biology, electron bifurcation, uses free energy from exergonic redox reactions to drive endergonic redox reactions. Flavin-based electron bifurcation furnishes low-potential electrons to demanding chemical reactions, such as reduction of dinitrogen to ammonia. We employed the heterodimeric flavoenzyme FixAB from the diazotrophic bacterium Rhodopseudomonas palustris to elucidate unique properties that underpin flavin-based electron bifurcation...
March 30, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29396026/the-effect-of-enteral-tube-feeding-in-cystic-fibrosis-a-registry-based-study
#7
Denis Libeert, Dimitri Declercq, Simeon Wanyama, Muriel Thomas, Sabine Van Daele, Frans De Baets, Stephanie Van Biervliet
BACKGROUND: Long-term effect of enteral tube feeding (ETF) in cystic fibrosis (CF) remains equivocal. METHODS: A Belgian CF registry based, retrospective, longitudinal study, evaluated the pre- and post- ETF (n = 113) clinical evolution and compared each patient with 2 age, gender, pancreatic status and genotype class-matched controls. RESULTS: At baseline ETF had a worse BMI z-score (p < 0.0001) and FEV1% (p < 0.0001) compared to controls...
March 2018: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/29390157/vacuolar-protein-degradation-via-autophagy-provides-substrates-to-amino-acid-catabolic-pathways-as-an-adaptive-response-to-sugar-starvation-in-arabidopsis-thaliana
#8
Takaaki Hirota, Masanori Izumi, Shinya Wada, Amane Makino, Hiroyuki Ishida
The vacuolar lytic degradation of proteins releases free amino acids (AAs) that plants can use instead of sugars for respiratory energy production. Autophagy is a major cellular process leading to the transport of proteins into the vacuole for degradation. Here, we examine the contribution of autophagy to the AA metabolism response to sugar starvation in mature leaves of Arabidopsis thaliana. During sugar starvation arising from the exposure of wild-type (WT) plants to darkness, autophagic transport of chloroplast stroma, which contains most of the proteins in a leaf, into the vacuolar lumen was induced within two days...
January 30, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29385059/multichannel-high-resolution-wide-swath-sar-imaging-for-hypersonic-air-vehicle-with-curved-trajectory
#9
Rui Zhou, Jinping Sun, Yuxin Hu, Yaolong Qi
Synthetic aperture radar (SAR) equipped on the hypersonic air vehicle in near space has many advantages over the conventional airborne SAR. However, its high-speed maneuvering characteristics with curved trajectory result in serious range migration, and exacerbate the contradiction between the high resolution and wide swath. To solve this problem, this paper establishes the imaging geometrical model matched with the flight trajectory of the hypersonic platform and the multichannel azimuth sampling model based on the displaced phase center antenna (DPCA) technology...
January 31, 2018: Sensors
https://www.readbyqxmd.com/read/29376952/-effect-of-different-nutritional-support-on-pancreatic-secretion-in-acute-pancreatitis
#10
E E Achkasov, A V Pugaev, Zh G Nabiyeva, S V Kalachev
AIM: To develop and justify optimal nutritional support in early phase of acute pancreatitis (AP). MATERIAL AND METHODS: 140 AP patients were enrolled. They were divided into groups depending on nutritional support: group I (n=70) - early enteral tube feeding (ETF) with balanced mixtures, group II (n=30) - early ETF with oligopeptide mixture, group III (n=40) - total parenteral nutrition (TPN). The subgroups were also isolated depending on medication: A - Octreotide, B - Quamatel, C - Octreotide + Quamatel...
2018: Khirurgiia
https://www.readbyqxmd.com/read/29366429/new-insights-on-ethambutol-targets-in-mycobacterium-tuberculosis
#11
Luciana Dias Ghiraldi-Lopes, Paula Aline Zanetti Campanerut-Sa, Geisa Paulino Caprini Evaristo, Jean Eduardo Meneguello, Adriana Fiorini, Vanessa Pietrowski Baldin, Emanuel Maltempi de Souza, Regiane Bertin de Lima Scodro, Vera Lucia Dias Siqueira, Rosilene Fressatti Cardoso
BACKGROUND: In recent years, very few effective drugs against Mycobacterium tuberculosis (M. tb) have emerged which motivates the research with drugs already used in the treatment of tuberculosis. EMB is a bacteriostatic drug that affects cell wall integrity, but the effects of this drug on bacilli are not fully exploited. OBJECTIVE: Based on the need to better investigate the complex mechanism of action of EMB, our study presented the proteome profile of M. tb after different times of EMB exposure, aiming to comprehend the dynamics of bacilli response to its effects...
January 24, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29352221/role-of-mettl20-in-regulating-%C3%AE-oxidation-and-heat-production-in-mice-under-fasting-or-ketogenic-conditions
#12
Tadahiro Shimazu, Tamio Furuse, Shabeesh Balan, Ikuko Yamada, Shuzo Okuno, Hiroko Iwanari, Takehiro Suzuki, Takao Hamakubo, Naoshi Dohmae, Takeo Yoshikawa, Shigeharu Wakana, Yoichi Shinkai
METTL20 is a seven-β-strand methyltransferase that is localised to the mitochondria and tri-methylates the electron transfer flavoprotein (ETF) β subunit (ETFB) at lysines 200 and 203. It has been shown that METTL20 decreases the ability of ETF to extract electrons from medium-chain acyl-coenzyme A (CoA) dehydrogenase (MCAD) and glutaryl-CoA dehydrogenase in vitro. METTL20-mediated methylation of ETFB influences the oxygen consumption rate in permeabilised mitochondria, suggesting that METTL20-mediated ETFB methylation may also play a regulatory role in mitochondrial metabolism...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329327/selection-of-housekeeping-genes-and-demonstration-of-rnai-in-cotton-leafhopper-amrasca-biguttula-biguttula-ishida
#13
Satnam Singh, Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Pankaj Rathore, Subba Reddy Palli
Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest of cotton and okra. Not much is known on this insect at molecular level due to lack of genomic and transcriptomic data. To prepare for functional genomic studies in this insect, we evaluated 15 common housekeeping genes (Tub, B-Tub, EF alpha, GADPH, UbiCF, RP13, Ubiq, G3PD, VATPase, Actin, 18s, 28s, TATA, ETF, SOD and Cytolytic actin) during different developmental stages and under starvation stress. We selected early (1st and 2nd), late (3rd and 4th) stage nymphs and adults for identification of stable housekeeping genes using geNorm, NormFinder, BestKeeper and RefFinder software...
2018: PloS One
https://www.readbyqxmd.com/read/29242111/autologous-transplantation-in-follicular-lymphoma-with-early-therapy-failure-a-national-lymphocare-study-and-center-for-international-blood-and-marrow-transplant-research-analysis
#14
Carla Casulo, Jonathan W Friedberg, Kwang W Ahn, Christopher Flowers, Alyssa DiGilio, Sonali M Smith, Sairah Ahmed, David Inwards, Mahmoud Aljurf, Andy I Chen, Hannah Choe, Jonathon Cohen, Edward Copelan, Umar Farooq, Timothy S Fenske, Cesar Freytes, Sameh Gaballa, Siddhartha Ganguly, Yogesh Jethava, Rammurti T Kamble, Vaishalee P Kenkre, Hillard Lazarus, Aleksandr Lazaryan, Richard F Olsson, Andrew R Rezvani, David Rizzieri, Sachiko Seo, Gunjan L Shah, Nina Shah, Melham Solh, Anna Sureda, Basem William, Aaron Cumpston, Andrew D Zelenetz, Brian K Link, Mehdi Hamadani
Patients with follicular lymphoma (FL) experiencing early therapy failure (ETF) within 2 years of frontline chemoimmunotherapy have poor overall survival (OS). We analyzed data from the Center for International Blood and Marrow Transplant Research (CIBMTR) and the National LymphoCare Study (NLCS) to determine whether autologous hematopoietic cell transplant (autoHCT) can improve outcomes in this high-risk FL subgroup. ETF was defined as failure to achieve at least partial response after frontline chemoimmunotherapy or lymphoma progression within 2 years of frontline chemoimmunotherapy...
December 11, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29200718/%C3%AE-glucosidase-inhibitory-activity-from-ethyl-acetate-extract-of-antidesma-bunius-l-spreng-stem-bark-containing-triterpenoids
#15
Marista Gilang Mauldina, Rani Sauriasari, Berna Elya
Background: Buni ( Antidesma bunius [L.] Spreng) has been used as a traditional antidiabetic agent in Asia. Objective: The mechanism of antidiabetic properties was studied in this study by determine its α-glucosidase inhibitory activity. Method: Inhibition of α-glucosidase was performed in all fraction of Buni stem bark with acarbose and miglitol as standards. The half maximal inhibitory concentration (IC50 ) value of acarbose and miglitol was 5...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29184297/the-relationship-between-non-affected-forelimb-exercise-and-recovery-after-focal-cerebral-ischemia-in-acute-phase
#16
Sang-Su Na, Seung-Kyu Kim, Nam-Jin Jung, Gak Hwangbo
[Purpose] In the present study, we hypothesized that exercise of the nonaffected forelimb in the early poststroke phase would stimulate the intact hemisphere, thereby influencing the hemisphere of the infarcted side and improving the performance of the hemiplegic limb. [Subjects and Methods] Adult male Sprague-Dawley rats (8-10 weeks of age, weighing 250-300 g, n=12) were used and randomly divided into 3 groups: nonaffected forelimb exercise for 3 days and treadmill exercise 7 days after ischemia (ETF, n=6), resting for 3 days and treadmill exercise 7 days after ischemia (ETN, n=6), and after ischemia 10 days resting group...
October 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/29130290/parent-patient-and-health-professional-perspectives-regarding-enteral-nutrition-in-paediatric-oncology
#17
Jennifer Cohen, Claire E Wakefield, Linda C Tapsell, Karen Walton, Richard J Cohn
AIM: Enteral tube feeding (ETF) is an important part of treatment for paediatric cancer patients. Without nutritional therapy, the prevalence of under-nutrition during treatment for childhood cancer may be as high as 50%. To ensure that the appropriate initiation of ETF is optimised, information on the views of key stakeholders regarding ETF is needed. METHODS: In total, 48 interviews were conducted with parents of paediatric cancer patients (n = 20), patients (n = 10) and members of the paediatric oncology health-care team (n = 18)...
November 2017: Nutrition & Dietetics: the Journal of the Dietitians Association of Australia
https://www.readbyqxmd.com/read/29122468/an-intronic-variation-in-slc52a1-causes-exon-skipping-and-transient-riboflavin-responsive-multiple-acyl-coa-dehydrogenation-deficiency
#18
Signe Mosegaard, Gitte Hoffmann Bruun, Karen Freund Flyvbjerg, Yngve Thomas Bliksrud, Niels Gregersen, Maja Dembic, Ellen Annexstad, Trine Tangeraas, Rikke Katrine Jentoft Olsen, Brage S Andresen
Vitamin B2, riboflavin is essential for cellular function, as it participates in a diversity of redox reactions central to human metabolism, through its role as precursor for the cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are electron carriers. The electron transfer flavoprotein (ETF) and its dehydrogenase (ETFDH), uses FAD as cofactor. The ETF and ETFDH are forming the electron transport pathway for many mitochondrial flavoprotein dehydrogenases involved in fatty acid, amino acid and choline metabolism...
December 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/29099232/a-novel-imaging-method-for-the-cartilaginous-eustachian-tube-lumen-computerized-tomography-during-the-forced-response-test
#19
Cuneyt M Alper, Tanya J Rath, Miriam S Teixeira, J Douglas Swarts
OBJECTIVES: In vivo imaging of the open cartilaginous Eustachian tube (ET) lumen by computed tomography (CT) scan during ET function (ETF) testing to establish new methodology. METHODS: Five adults underwent unilateral ETF testing of an ear with a nonintact tympanic membrane using the forced response test (FRT) to measure the opening pressure (PO), steady state pressure (PS), and flow conductance (CS). Then at baseline and during the PS phase of the FRT, a temporal-bone CT scan with continuous 0...
January 2018: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/29066244/reliability-of-assessing-postural-control-during-seated-balancing-using-a-physical-human-robot-interaction
#20
Ahmed Ramadan, Jacek Cholewicki, Clark J Radcliffe, John M Popovich, N Peter Reeves, Jongeun Choi
This study evaluated the within- and between-visit reliability of a seated balance test for quantifying trunk motor control using input-output data. Thirty healthy subjects performed a seated balance test under three conditions: eyes open (EO), eyes closed (EC), and eyes closed with vibration to the lumbar muscles (VIB). Each subject performed three trials of each condition on three different visits. The seated balance test utilized a torque-controlled robotic seat, which together with a sitting subject resulted in a physical human-robot interaction (pHRI) (two degrees-of-freedom with upper and lower body rotations)...
November 7, 2017: Journal of Biomechanics
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