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https://www.readbyqxmd.com/read/28933654/commonalities-and-differences-in-plants-deficient-in-autophagy-and-alternative-pathways-of-respiration-on-response-to-extended-darkness
#1
Jessica A S Barros, João Henrique F Cavalcanti, David B Medeiros, Adriano Nunes-Nesi, Tamar Avin-Wittenberg, Alisdair R Fernie, Wagner L Araújo
Autophagy is a highly conserved cellular mechanism in eukaryotes allowing the degradation of cell constituents. It is of crucial significance in both cellular homeostasis and nutrient recycling. During energy limited conditions plant cells can metabolize alternative respiratory substrates, such as amino acids, providing electrons to the mitochondrial metabolism via the tricarboxylic acid (TCA) cycle or electron transfer flavoprotein/ electron transfer flavoprotein ubiquinone oxidoreductase (ETF/ETFQO) system...
September 21, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28919081/body-weight-and-body-mass-index-in-patients-with-end-stage-cystic-fibrosis-stabilize-after-the-start-of-enteral-tube-feeding
#2
Francis M Hollander, Nicole M de Roos, Gerdien Belle van Meerkerk, Ferdinand Teding van Berkhout, Harry G M Heijerman, Ed A van de Graaf
BACKGROUND: Enteral tube feeding (ETF) is widely used in patients with cystic fibrosis (CF) and end-stage lung disease, but previous studies have been limited to investigating whether ETF improves outcomes in patients with moderately or mildly impaired pulmonary function. OBJECTIVE: This study investigated body weight, body mass index (BMI; calculated as kg/m(2)), pulmonary function, and the presence of CF-related diabetes before and after the start of ETF. DESIGN: This was a retrospective observational study...
September 11, 2017: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/28827360/coupling-between-d-3-phosphoglycerate-dehydrogenase-and-d-2-hydroxyglutarate-dehydrogenase-drives-bacterial-l-serine-synthesis
#3
Wen Zhang, Manman Zhang, Chao Gao, Yipeng Zhang, Yongsheng Ge, Shiting Guo, Xiaoting Guo, Zikang Zhou, Qiuyuan Liu, Yingxin Zhang, Cuiqing Ma, Fei Tao, Ping Xu
l-Serine biosynthesis, a crucial metabolic process in most domains of life, is initiated by d-3-phosphoglycerate (d-3-PG) dehydrogenation, a thermodynamically unfavorable reaction catalyzed by d-3-PG dehydrogenase (SerA). d-2-Hydroxyglutarate (d-2-HG) is traditionally viewed as an abnormal metabolite associated with cancer and neurometabolic disorders. Here, we reveal that bacterial anabolism and catabolism of d-2-HG are involved in l-serine biosynthesis in Pseudomonas stutzeri A1501 and Pseudomonas aeruginosa PAO1...
August 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28817787/evaluation-of-the-electron-transfer-flavoprotein-etf-as-an-antibacterial-target-in-burkholderia-cenocepacia
#4
Maria S Stietz, Christina Lopez, Osasumwen Osifo, Marcelo Tolmasky, Silvia T Cardona
There are hundreds of essential genes in multidrug resistant bacterial genomes, but only a few of their products are exploited as antibacterial targets. An example is the electron flavoprotein (ETF) which is required for growth and viability in <i>Burkholderia cenocepacia</i>. Here, we evaluated ETF as an antibiotic target for <i>Burkholderia cepacia</i> complex (Bcc). Depletion of the bacterial ETF during infection of <i>Caenorhabditis elegans</i> significantly extended survival of the nematodes, proving that ETF is essential for survival of <i>B...
August 17, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28808132/defining-electron-bifurcation-in-the-electron-transferring-flavoprotein-family
#5
Amaya M Garcia Costas, Saroj Poudel, Anne-Frances Miller, Gerrit J Schut, Rhesa N Ledbetter, Kathryn R Fixen, Lance C Seefeldt, Michael W W Adams, Caroline S Harwood, Eric S Boyd, John W Peters
Electron bifurcation is the coupling of exergonic and endergonic redox reactions to simultaneously generate (or utilize) low and high potential electrons. It is the third recognized form of energy conservation in biology and has recently been described in select electron transferring flavoproteins (Etfs). Etfs are flavin-containing heterodimers best known for donating electrons derived from fatty acid and amino acid oxidation to an electron transfer respiratory chain via ETF quinone oxidoreductase. Canonical examples contain a flavin adenine dinucleotide (FAD) that is involved in electron transfer as well as a non-redox active adenosine monophosphate (AMP)...
August 14, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28807745/acute-toxicity-of-functionalized-single-wall-carbon-nanotubes-a-biochemical-histopathologic-and-proteomics-approach
#6
Homa Ahmadi, Mohammad Ramezani, Rezvan Yazdian-Robati, Behzad Behnam, Kamal Razavi Azarkhiavi, Azadeh Hashem Nia, Ahad Mokhtarzadeh, Maryam Matbou Riahi, Bibi Marjan Razavi, Khalil Abnous
Recently carbon nanotubes (CNTs) showed promising potentials in different biomedical applications but their safe use in humans and probable toxicities are still challenging. The aim of this study was to determine the acute toxicity of functionalized single walled carbon nanotubes (SWCNTs). In this project, PEGylated and Tween functionalized SWCNTs were prepared. BALB/c mice were randomly divided into nine groups, including PEGylated SWCNTs (75,150μg/mouse) and PEG, Tween80 suspended SWCNTs, Tween 80 and a control group (intact mice)...
August 12, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28771207/hot-press-as-a-sustainable-direct-recycling-technique-of-aluminium-mechanical-properties-and-surface-integrity
#7
Nur Kamilah Yusuf, Mohd Amri Lajis, Azlan Ahmad
Meltless recycling technique has been utilized to overcome the lack of primary resources, focusing on reducing the usage of energy and materials. Hot press was proposed as a novel direct recycling technique which results in astoundingly low energy usage in contrast with conventional recycling. The aim of this study is to prove the technical feasibility of this approach by characterizing the recycled samples. For this purpose, AA6061 aluminium chips were recycled by utilizing hot press process under various operating temperature (Ts = 430, 480, and 530 °C) and holding times (ts = 60, 90, and 120 min)...
August 3, 2017: Materials
https://www.readbyqxmd.com/read/28767134/the-differential-impact-of-amino-acids-on-oxphos-system-activity-following-carbohydrate-starvation-in-arabidopsis-cell-suspensions
#8
João Henrique F Cavalcanti, Carla G S Quinhones, Peter Schertl, Danielle S Brito, Holger Eubel, Tatjana Hildebrandt, Adriano Nunes-Nesi, Hans-Peter Braun, Wagner L Araújo
Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid (TCA) cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons via the ETF/ETFQO complex to the mitochondrial electron transport chain (mETC). Given to this, here we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids...
August 2, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28699793/predictors-of-early-treatment-failure-following-initial-therapy-for-systemic-immunoglobulin-light-chain-amyloidosis
#9
Nidhi Tandon, Surbhi Sidana, S Vincent Rajkumar, Angela Dispenzieri, Morie A Gertz, Martha Q Lacy, Robert A Kyle, Francis K Buadi, David Dingli, Suzanne R Hayman, Amie L Fonder, Miriam A Hobbs, Wilson I Gonsalves, Prashant Kapoor, Yi Lisa Hwa, Nelson Leung, Ronald S Go, John A Lust, Stephen J Russell, Steven R Zeldenrust, Shaji K Kumar
We analysed factors predicting early treatment failure (ETF), after first-line therapy for light-chain amyloidosis (AL). AL amyloidosis patients seen at Mayo Clinic within 90 days of diagnosis, from 2006 to 2015, excluding those who died within 3 months of initial therapy, were analysed retrospectively. ETF was defined as progression requiring treatment change or death within 12 (ETF12) or 24 (ETF24) months of first-line treatment. Non-ETF included those with a follow-up of more than 12 or 24 months who had progression beyond 12 or 24 months...
July 12, 2017: Amyloid: the International Journal of Experimental and Clinical Investigation
https://www.readbyqxmd.com/read/28638803/peptide-nucleic-acid-knockdown-and-intra-host-cell-complementation-of-ehrlichia-type-iv-secretion-system-effector
#10
Pratibha Sharma, Omid Teymournejad, Yasuko Rikihisa
Survival of Ehrlichia chaffeensis depends on obligatory intracellular infection. One of the barriers to E. chaffeensis research progress has been the inability, using conventional techniques, to generate knock-out mutants for genes essential for intracellular infection. This study examined the use of Peptide Nucleic Acids (PNAs) technology to interrupt type IV secretion system (T4SS) effector protein expression in E. chaffeensis followed by intracellular complementation of the effector to determine its requirement for infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28611224/random-subsets-of-structured-deterministic-frames-have-manova-spectra
#11
Marina Haikin, Ram Zamir, Matan Gavish
We draw a random subset of [Formula: see text] rows from a frame with [Formula: see text] rows (vectors) and [Formula: see text] columns (dimensions), where [Formula: see text] and [Formula: see text] are proportional to [Formula: see text] For a variety of important deterministic equiangular tight frames (ETFs) and tight non-ETFs, we consider the distribution of singular values of the [Formula: see text]-subset matrix. We observe that, for large [Formula: see text], they can be precisely described by a known probability distribution-Wachter's MANOVA (multivariate ANOVA) spectral distribution, a phenomenon that was previously known only for two types of random frames...
June 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28592294/exercise-in-claudicants-increase-or-decrease-walking-ability-and-the-response-relates-to-mitochondrial-function
#12
Michel van Schaardenburgh, Martin Wohlwend, Øivind Rognmo, Erney J R Mattsson
BACKGROUND: Exercise of patients with intermittent claudication improves walking performance. Exercise does not usually increase blood flow, but seems to increase muscle mitochondrial enzyme activities. Although exercise is beneficial in most patients, it might be harmful in some. The mitochondrial response to exercise might therefore differ between patients. Our hypothesis was that changes in walking performance relate to changes in mitochondrial function after 8 weeks of exercise. At a subgroup level, negative responders decrease and positive responders increase mitochondrial capacity...
June 7, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28417225/comparative-proteomic-analysis-provides-insight-into-10-hydroxy-2-decenoic-acid-biosynthesis-in-honey-bee-workers
#13
Xiao-Hui Yang, Shi-Fa Yang, Rui-Ming Wang
10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis was performed in the MGs of workers with different 10-HDA production. In total, 59 up-regulated protein species representing 45 unique proteins were identified in high 10-HDA-producing workers by 2-DE-MALDI-TOF/TOF MS...
April 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28362573/ultrasound-assessed-gastric-antral-area-correlates-with-aspirated-tube-feed-volume-in-enterally-fed-critically-ill-patients
#14
Vibhu Sharma, Deepthi Gudivada, Renaud Gueret, John Bailitz
BACKGROUND: Enteral tube feed (ETF) intolerance occurs frequently in hospitalized patients and more so in critically ill patients. Most critical care nurses continue to assess gastric residual volume (GRV), especially among those with a history of ETF intolerance. We hypothesized that ultrasound assessment of GRV correlates directly with aspirated tube feed volume. METHODS: This was a prospective cohort study of a convenience sample of critically ill mechanically ventilated patients admitted to an intensive care unit receiving ETF...
April 2017: Nutrition in Clinical Practice
https://www.readbyqxmd.com/read/28222553/dual-afferent-sensory-input-training-for-voluntary-movement-after-stroke-a-pilot-randomized-controlled-study
#15
Seahyun Bae, Kyung-Yoon Kim
BACKGROUND: Stimulation through afferent sensory input is necessary to improve voluntary functional movement in stroke patients. OBJECTIVES: Dual-afferent sensory input, which combines electromyography-triggered functional electric stimulation (ETFES) and action observation, was investigated to determine its effects on voluntary movements in stroke patients. METHODS: This study was conducted on 18 patients with left hemiplegia diagnosed between 6 and 24 months prior...
2017: NeuroRehabilitation
https://www.readbyqxmd.com/read/27932700/predictors-of-enteral-tube-feeding-in-hospitalized-older-adults
#16
Milton Roberto Furst Crenitte, Thiago Junqueira Avelino-Silva, Daniel Apolinario, Jose Antonio Esper Curiati, Flavia Campora, Wilson Jacob-Filho
BACKGROUND: Despite general recognition that enteral tube feeding (ETF) is frequently employed in long-term care facilities and patients with dementia, remarkably little research has determined which factors are associated with its use in acutely ill older adults. In this study, we aimed to investigate determinants of ETF introduction in hospitalized older adults. METHODS: We examined a retrospective cohort of acutely ill patients, aged 60 years and older, admitted to a university hospital's geriatric ward from 2014-2015, in São Paulo, Brazil...
December 8, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27916500/achieving-ethanol-type-fermentation-for-hydrogen-production-in-a-granular-sludge-system-by-aeration
#17
Song Zhang, Min Liu, Ying Chen, Yu-Ting Pan
To investigate the effects of aeration on hydrogen-producing granular system, experiments were performed in two laboratory-scale anaerobic internal circulation hydrogen production (AICHP) reactors. The preliminary experiment of Reactor 1 showed that direct aeration was beneficial to enhancing hydrogen production. After the direct aeration was implied in Reactor 2, hydrogen production rate (HPR) and hydrogen content were increased by 100% and 60%, respectively. In addition, mixed-acid fermentation was transformed into typical ethanol-type fermentation (ETF)...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27771746/identification-and-functional-analysis-of-the-gtpv-bidirectional-promoter-region
#18
Hui Zhang, Zhihua Sun, Na Zhang, Zhiqiang Li, Pengyan Wang, Qiang Fu, Yan Ren, Xuehua Shao, Yu Zhang, Zhiru Guo, Chuangfu Chen
The goat pox chick embryo-attenuated virus (GTPV) has been developed as an effective vaccine that can elicit protective immune responses. It possesses a large genome and a robust ability to express exogenous genes. Thus, this virus is an ideal vector for recombinant live vaccines for infectious diseases in ruminant animals. In this study, we identified a novel bidirectional promoter region of GTPV through screening named PbVV(±). PbVV(±) is located between ETF-l and VITF-3, which are transcribed in opposite directions...
March 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/27701823/differential-proteomic-analysis-of-the-metabolic-network-of-the-marine-sulfate-reducer-desulfobacterium-autotrophicum-hrm2
#19
Marvin Dörries, Lars Wöhlbrand, Ralf Rabus
The marine sulfate-reducing bacterium Desulfobacterium autotrophicum HRM2 belongs to the deltaproteobacterial family Desulfobacteraceae and stands out for its capacity of facultative chemolithoautotrophic growth (next to heterotrophy). Here, proteomics-driven metabolic reconstruction was based on a combination of 2D-DIGE, shotgun proteomics, and analysis of the membrane protein enriched fraction applied to eight different substrate adaptation conditions (seven aliphatic compounds plus H2 /CO2 ). In total, 1344 different proteins were identified (∼27% of the 4947 genome-predicted), from which a complex metabolic network was reconstructed consisting of 136 proteins (124 detected; ∼91%)...
November 2016: Proteomics
https://www.readbyqxmd.com/read/27673344/electron-beam-lithographic-grafting-of-functional-polymer-structures-from-fluoropolymer-substrates
#20
Katarzyna Gajos, Vitaliy A Guzenko, Matthias Dübner, Jakub Haberko, Andrzej Budkowski, Celestino Padeste
Well-defined submicrometer structures of poly(dimethylaminoethyl methacrylate) (PDMAEMA) were grafted from 100 μm thick films of poly(ethene-alt-tetrafluoroethene) after electron-beam lithographic exposure. To explore the possibilities and limits of the method under different exposure conditions, two different acceleration voltages (2.5 and 100 keV) were employed. First, the influence of electron energy and dose on the extent of grafting and on the structure's morphology was determined via atomic force microscopy...
October 7, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
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