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https://www.readbyqxmd.com/read/27771746/identification-and-functional-analysis-of-the-gtpv-bidirectional-promoter-region
#1
Hui Zhang, Zhihua Sun, Na Zhang, Zhiqiang Li, Pengyan Wang, Qiang Fu, Yan Ren, Xuehua Shao, Yu Zhang, Zhiru Guo, Chuangfu Chen
The goat pox chick embryo-attenuated virus (GTPV) has been developed as an effective vaccine that can elicit protective immune responses. It possesses a large genome and a robust ability to express exogenous genes. Thus, this virus is an ideal vector for recombinant live vaccines for infectious diseases in ruminant animals. In this study, we identified a novel bidirectional promoter region of GTPV through screening named PbVV(±). PbVV(±) is located between ETF-l and VITF-3, which are transcribed in opposite directions...
October 22, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27701823/differential-proteomic-analysis-of-the-metabolic-network-of-the-marine-sulfate-reducer-desulfobacterium-autotrophicum-hrm2
#2
Marvin Dörries, Lars Wöhlbrand, Ralf Rabus
The marine sulfate-reducing bacterium Desulfobacterium autotrophicum HRM2 belongs to the deltaproteobacterial family Desulfobacteraceae and stands out for its capacity of facultative chemolithoautotrophic growth (next to heterotrophy). Here, proteomics-driven metabolic reconstruction was based on a combination of 2D DIGE, shotgun proteomics and analysis of the membrane protein-enriched fraction applied to 8 different substrate adaptation conditions (7 aliphatic compounds plus H2 /CO2 ). In total 1,344 different proteins were identified (∼27% of the 4,947 genome-predicted) from which a complex metabolic network was reconstructed consisting of 136 proteins (124 detected; ∼91%)...
October 4, 2016: Proteomics
https://www.readbyqxmd.com/read/27673344/electron-beam-lithographic-grafting-of-functional-polymer-structures-from-fluoropolymer-substrates
#3
Katarzyna Gajos, Vitaliy A Guzenko, Matthias Dübner, Jakub Haberko, Andrzej Budkowski, Celestino Padeste
Well-defined submicrometer structures of poly(dimethylaminoethyl methacrylate) (PDMAEMA) were grafted from 100 μm thick films of poly(ethene-alt-tetrafluoroethene) after electron-beam lithographic exposure. To explore the possibilities and limits of the method under different exposure conditions, two different acceleration voltages (2.5 and 100 keV) were employed. First, the influence of electron energy and dose on the extent of grafting and on the structure's morphology was determined via atomic force microscopy...
October 7, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27541856/ehrlichia-secretes-etf-1-to-induce-autophagy-and-capture-nutrients-for-its-growth-through-rab5-and-class-iii-phosphatidylinositol-3-kinase
#4
Mingqun Lin, Hongyan Liu, Qingming Xiong, Hua Niu, Zhihui Cheng, Akitsugu Yamamoto, Yasuko Rikihisa
Ehrlichia chaffeensis is an obligatory intracellular bacterium that causes a potentially fatal emerging zoonosis, human monocytic ehrlichiosis. E. chaffeensis has a limited capacity for biosynthesis and metabolism and thus depends mostly on host-synthesized nutrients for growth. Although the host cell cytoplasm is rich with these nutrients, as E. chaffeensis is confined within the early endosome-like membrane-bound compartment, only host nutrients that enter the compartment can be used by this bacterium. How this occurs is unknown...
November 2016: Autophagy
https://www.readbyqxmd.com/read/27431485/comparative-genomics-of-syntrophic-branched-chain-fatty-acid-degrading-bacteria
#5
Takashi Narihiro, Masaru K Nobu, Hideyuki Tamaki, Yoichi Kamagata, Yuji Sekiguchi, Wen-Tso Liu
The syntrophic degradation of branched-chain fatty acids (BCFAs) such as 2-methylbutyrate and isobutyrate is an essential step in the production of methane from proteins/amino acids in anaerobic ecosystems. While a few syntrophic BCFA-degrading bacteria have been isolated, their metabolic pathways in BCFA and short-chain fatty acid (SCFA) degradation as well as energy conservation systems remain unclear. In an attempt to identify these pathways, we herein performed comparative genomics of three syntrophic bacteria: 2-methylbutyrate-degrading "Syntrophomonas wolfei subsp...
September 29, 2016: Microbes and Environments
https://www.readbyqxmd.com/read/27427709/morphological-study-of-poly-vinylbenzyl-chloride-grafted-poly-ethylene-co-tetrafluoroethylene-etfe-g-pvbc-films-using-small-angle-neutron-scattering-analysis
#6
J M Song, B S Ko, J Y Sohn, J Shin
In this study, the effect of degree of the grafting and crosslinking on the morphology of the crystalline domain in poly(vinylbenzyl chloride)-grafted poly(ethylene-co-tetrafluoroethylene) [ETFE-g-PVBC] films was investigated using a SANS (small-angle neutron scattering) analysis. The grafted films can be used as a precursor for ion-exchange membrane. ETFE-g-PVBC films with various degrees of cross-linking were prepared by a simultaneous irradiation grafting of vinylbenzyl chloride (VBC) and divinylbenzene (DVB) onto an ETFE film...
June 2016: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/27367048/phenotypic-characterization-and-genetic-dissection-of-growth-period-traits-in-soybean-glycine-max-using-association-mapping
#7
Zhangxiong Liu, Huihui Li, Xuhong Fan, Wen Huang, Jiyu Yang, Candong Li, Zixiang Wen, Yinghui Li, Rongxia Guan, Yong Guo, Ruzhen Chang, Dechun Wang, Shuming Wang, Li-Juan Qiu
The growth period traits are important traits that affect soybean yield. The insights into the genetic basis of growth period traits can provide theoretical basis for cultivated area division, rational distribution, and molecular breeding for soybean varieties. In this study, genome-wide association analysis (GWAS) was exploited to detect the quantitative trait loci (QTL) for number of days to flowering (ETF), number of days from flowering to maturity (FTM), and number of days to maturity (ETM) using 4032 single nucleotide polymorphism (SNP) markers with 146 cultivars mainly from Northeast China...
2016: PloS One
https://www.readbyqxmd.com/read/27339419/gene-network-activity-in-cultivated-primary-hepatocytes-is-highly-similar-to-diseased-mammalian-liver-tissue
#8
Patricio Godoy, Agata Widera, Wolfgang Schmidt-Heck, Gisela Campos, Christoph Meyer, Cristina Cadenas, Raymond Reif, Regina Stöber, Seddik Hammad, Larissa Pütter, Kathrin Gianmoena, Rosemarie Marchan, Ahmed Ghallab, Karolina Edlund, Andreas Nüssler, Wolfgang E Thasler, Georg Damm, Daniel Seehofer, Thomas S Weiss, Olaf Dirsch, Uta Dahmen, Rolf Gebhardt, Umesh Chaudhari, Kesavan Meganathan, Agapios Sachinidis, Jens Kelm, Ute Hofmann, René P Zahedi, Reinhard Guthke, Nils Blüthgen, Steven Dooley, Jan G Hengstler
It is well known that isolation and cultivation of primary hepatocytes cause major gene expression alterations. In the present genome-wide, time-resolved study of cultivated human and mouse hepatocytes, we made the observation that expression changes in culture strongly resemble alterations in liver diseases. Hepatocytes of both species were cultivated in collagen sandwich and in monolayer conditions. Genome-wide data were also obtained from human NAFLD, cirrhosis, HCC and hepatitis B virus-infected tissue as well as mouse livers after partial hepatectomy, CCl4 intoxication, obesity, HCC and LPS...
October 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27232886/stability-and-degradation-mechanisms-of-radiation-grafted-polymer-electrolyte-membranes-for-water-electrolysis
#9
Albert Albert, Tim Lochner, Thomas J Schmidt, L Gubler
Radiation-grafted membranes are a promising alternative to commercial membranes for water electrolyzers, since they exhibit lower hydrogen crossover and area resistance, better mechanical properties, and are of potentially lower cost than perfluoroalkylsulfonic acid membranes, such as Nafion. Stability is an important factor in view of the expected lifetime of 40 000 h or more of an electrolyzer. In this study, combinations of styrene (St), α-methylstyrene (AMS), acrylonitrile (AN), and 1,3-diisopropenylbenzene (DiPB) are cografted into 50 μm preirradiated poly(ethylene-co-tetrafluoroethylene) (ETFE) base film, followed by sulfonation to produce radiation-grafted membranes...
June 22, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27206643/the-fabrication-of-nanoimprinted-p3ht-nanograting-by-patterned-etfe-mold-at-room-temperature-and-its-application-for-solar-cell
#10
Guangzhu Ding, Kaixuan Wang, Xiaohui Li, Qing Chen, Zhijun Hu, Jieping Liu
Nanoimprinting lithography (NIL) is investigated as a promising method to define nanostructure; however, finding a practical method to achieve large area patterning of conjugated polymer remains a challenge. We demonstrate here that a simple and cost-effective technique is proposed to fabricate the nanoimprinted P3HT nanograting by solvent-assisted room temperature NIL (SART-NIL) method with patterned ETFE film as mold. The patterned ETFE template is produced by embossing ETFE film into a patterned silicon master and is used as template to transfer nanogratings during the SART-NIL process...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27198766/methanogenic-paraffin-degradation-proceeds-via-alkane-addition-to-fumarate-by-smithella-spp-mediated-by-a-syntrophic-coupling-with-hydrogenotrophic-methanogens
#11
Boris Wawrik, Christopher R Marks, Irene A Davidova, Michael J McInerney, Shane Pruitt, Kathleen E Duncan, Joseph M Suflita, Amy V Callaghan
Anaerobic microbial biodegradation of recalcitrant, water-insoluble substrates, such as paraffins, presents unique metabolic challenges. To elucidate this process, a methanogenic consortium capable of mineralizing long-chain n-paraffins (C28 -C50 ) was enriched from San Diego Bay sediment. Analysis of 16S rRNA genes indicated the dominance of Syntrophobacterales (43%) and Methanomicrobiales (26%). Metagenomic sequencing allowed draft genome assembly of dominant uncultivated community members belonging to the bacterial genus Smithella and the archaeal genera Methanoculleus and Methanosaeta...
September 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27136103/superamphiphobic-and-electroactive-nanocomposite-toward-self-cleaning-antiwear-and-anticorrosion-coatings
#12
Ruixia Yuan, Shiqi Wu, Peng Yu, Baohui Wang, Liwen Mu, Xiguang Zhang, Yixing Zhu, Bing Wang, Huaiyuan Wang, Jiahua Zhu
Multifunctional coatings are in urgent demand in emerging fields. In this work, nanocomposite coatings with extraordinary self-cleaning, antiwear, and anticorrosion properties were prepared on aluminum substrate by a facile spraying technique. Core-shell structured polyaniline/functionalized carbon nanotubes (PANI/fCNTs) composite and nanosized silica were synergistically integrated into ethylene tetrafluoroethylene (ETFE) matrix to construct lotus-leaf-like structures, and 1H,1H,2H,2H- perfluorooctyltriethoxysilane (POTS) was used to decrease the surface energy...
May 18, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27113712/functional-characterization-of-electron-transferring-flavoprotein-and-its-dehydrogenase-required-for-fungal-development-and-plant-infection-by-the-rice-blast-fungus
#13
Ya Li, Jindong Zhu, Jiexiong Hu, Xiuli Meng, Qi Zhang, Kunpeng Zhu, Xiaomin Chen, Xuehang Chen, Guangpu Li, Zonghua Wang, Guodong Lu
Electron-transferring flavoprotein (ETF) and its dehydrogenase (ETFDH) are highly conserved electron carriers which mainly function in mitochondrial fatty acid β oxidation. Here, we report the identification and characterization of ETF α and β subunit encoding genes (ETFA and ETFB) and ETFDH encoding gene (ETFDH) in the rice blast fungus Magnaporthe oryzae. It was demonstrated that, by impacting fatty acid metabolism, ETF and ETFDH mutations led to severe growth and conidiation defects, which could be largely rescued by exogenous acetate or carbonate...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27085476/cardiolipin-fatty-acid-remodeling-regulates-mitochondrial-function-by-modifying-the-electron-entry-point-in-the-respiratory-chain
#14
Aurelia Vergeade, Clinton C Bertram, Alfiya T Bikineyeva, William E Zackert, Sandra S Zinkel, James M May, Sergey I Dikalov, L Jackson Roberts, Olivier Boutaud
Modifications of cardiolipin (CL) levels or compositions are associated with changes in mitochondrial function in a wide range of pathologies. We have made the discovery that acetaminophen remodels CL fatty acids composition from tetralinoleoyl to linoleoyltrioleoyl-CL, a remodeling that is associated with decreased mitochondrial respiration. Our data show that CL remodeling causes a shift in electron entry from complex II to the β-oxidation electron transfer flavoprotein quinone oxidoreductase (ETF/QOR) pathway...
May 2016: Mitochondrion
https://www.readbyqxmd.com/read/27048649/reduction-of-flavodoxin-by-electron-bifurcation-and-sodium-ion-dependent-reoxidation-by-nad-catalyzed-by-ferredoxin-nad-reductase-rnf
#15
Nilanjan Pal Chowdhury, Katharina Klomann, Andreas Seubert, Wolfgang Buckel
Electron-transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) from Acidaminococcus fermentans catalyze the endergonic reduction of ferredoxin by NADH, which is also driven by the concomitant reduction of crotonyl-CoA by NADH, a process called electron bifurcation. Here we show that recombinant flavodoxin from A. fermentans produced in Escherichia coli can replace ferredoxin with almost equal efficiency. After complete reduction of the yellow quinone to the blue semiquinone, a second 1.4 times faster electron transfer affords the colorless hydroquinone...
June 3, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27035869/the-decreased-expression-of-electron-transfer-flavoprotein-%C3%AE-is-associated-with-tubular-cell-apoptosis-in-diabetic-nephropathy
#16
Hua Wang, Haojun Zhang, Xiaohong Chen, Tingting Zhao, Qin Kong, Meihua Yan, Bingxuan Zhang, Sifan Sun, Hui-Yao Lan, Ning Li, Ping Li
Tubular injury is closely correlated with the development of progressive diabetic nephropathy (DN), particularly in cases of type 2 diabetes. The apoptosis of tubular cells has been recognized as a major cause of tubular atrophy, followed by tubulointerstitial fibrosis. Electron transfer flavoprotein β (ETFβ) is known as an important electron acceptor in energy metabolism, but its role in DN was not fully understood. In the present study, we examined the expression pattern of ETFβ using diabetic kidney samples and further investigated ETFβ involvement in tubular epithelial cell (TEC) apoptosis...
May 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/26929405/the-mettl20-homologue-from-agrobacterium-tumefaciens-is-a-dual-specificity-protein-lysine-methyltransferase-that-targets-ribosomal-protein-l7-l12-and-the-%C3%AE-subunit-of-electron-transfer-flavoprotein-etf%C3%AE
#17
Jędrzej Małecki, Helge-André Dahl, Anders Moen, Erna Davydova, Pål Ø Falnes
Human METTL20 is a mitochondrial, lysine-specific methyltransferase that methylates the β-subunit of electron transfer flavoprotein (ETFβ). Interestingly, putative METTL20 orthologues are found in a subset of α-proteobacteria, including Agrobacterium tumefaciens Using an activity-based approach, we identified in bacterial extracts two substrates of recombinant METTL20 from A. tumefaciens (AtMETTL20), namely ETFβ and the ribosomal protein RpL7/L12. We show that AtMETTL20, analogous to the human enzyme, methylates ETFβ on Lys-193 and Lys-196 both in vitro and in vivo ETF plays a key role in mediating electron transfer from various dehydrogenases, and we found that its electron transferring ability was diminished by AtMETTL20-mediated methylation of ETFβ...
April 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26919259/anthraphane-an-anthracene-based-propeller-shaped-d-3h-symmetric-hydrocarbon-cyclophane-and-its-layered-single-crystal-structures
#18
Marco Servalli, Nils Trapp, Michael Wörle, Frank-Gerrit Klärner
The novel hydrocarbon propeller-shaped D3h-symmetric cyclophane (3), "anthraphane", was prepared through a revisited and optimized gram-scale synthesis of the key building block anthracene-1,8-ditriflate 7. Anthraphane has a high tendency to crystallize and single crystals in size ranges of 100-200 μm are easily obtained from different solvents. The crystallization behavior of 3 was extensively studied to unravel packing motifs and determine whether the packing can be steered into a desired direction, so to allow topochemical photopolymerization...
March 18, 2016: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/26850741/prevalence-outcomes-and-management-of-enteral-tube-feeding-intolerance-a-retrospective-cohort-study-in-a-tertiary-center
#19
Kailun Wang, Kerry McIlroy, Lindsay D Plank, Max S Petrov, John A Windsor
BACKGROUND: Enteral tube feeding (ETF) is the most common form of artificial feeding in hospitalized patients, and the development of intolerance (ETFI) is the most common complication. This study aimed to determine the prevalence of ETFI, the clinical consequences, and the current management approach to ETFI in hospitalized adult patients. MATERIALS AND METHODS: Adult patients receiving ETF were identified from a prospective database in the Nutrition Services at Auckland City Hospital...
February 5, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/26850220/aryl-aryl-interactions-in-designed-peptide-folds-spectroscopic-characteristics-and-placement-issues-for-optimal-structure-stabilization
#20
Jordan M Anderson, Brandon Kier, Brice Jurban, Aimee Byrne, Irene Shu, Lisa A Eidenschink, Alexander A Shcherbakov, Mike Hudson, R M Fesinmeyer, Niels H Andersen
We have extended our studies of Trp/Trp to other Aryl/Aryl through-space interactions that stabilize hairpins and other small polypeptide folds. Herein we detail the NMR and CD spectroscopic features of these types of interactions. NMR data remains the best diagnostic for characterizing the common T-shape orientation. Designated as an edge-to-face (EtF or FtE) interaction, large ring current shifts are produced at the edge aryl ring hydrogens and, in most cases, large exciton couplets appear in the far UV circular dichroic (CD) spectrum...
February 6, 2016: Biopolymers
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