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https://www.readbyqxmd.com/read/29234180/role-of-the-nitrergic-system-of-the-cuneiform-nucleus-in-cardiovascular-responses-in-urethane-anesthetized-male-rats
#1
Esmaeil Farrokhi, Mohammad Naser Shafei, Abolfazl Khajavirad, Mahmoud Hosseini, Ali Reza Ebrahimzadeh Bideskan
Background: The presence of nitric oxide (NO) in the cuneiform nucleus (CnF) has been previously shown. In this study, NG-nitro-L-arginine methyl ester (L-NAME) (an inhibitor of NO synthase), L-arginine (L-Arg) (a precursor of NO), and sodium nitroprusside (SNP) (a donor of NO) were microinjected into the CnF and cardiovascular responses were investigated. Methods: Seventy male rats were divided into 7 groups (n=10 each): 1) saline, 2 and 3) L-NAME (30 and 90 nmol), 4 and 5) L-Arg (20 and 60 nmol), and 6 and 7) SNP (9 and 27 nmol)...
September 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29230833/causes-of-iron-overload-in-blood-donors-a-clinical-study
#2
A H Laursen, O W Bjerrum, L Friis-Hansen, T O Hansen, J L Marott, K Magnussen
BACKGROUND AND OBJECTIVES: Despite the obligate iron loss from blood donation, some donors present with hyperferritinaemia that can result from a wide range of acute and chronic conditions including hereditary haemochromatosis (HH). The objective of our study was to investigate the causes of hyperferritinaemia in the blood donor population and explore the value of extensive HH mutational analyses. MATERIALS AND METHODS: Forty-nine consecutive donors (f = 6, m = 43) were included prospectively from the Capital Regional Blood Center...
December 12, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/29227341/characterization-of-corneal-endothelial-injury-during-penetrating-keratoplasty
#3
Thomas A Vo, Zack Oakey, Sumit Garg
PURPOSE: To characterize corneal endothelial injury during penetrating keratoplasty in a controlled wet laboratory environment using human tissue. To identify potential areas or steps within surgery in which insult to the corneal endothelium may be most affected by trauma during routine penetrating keratoplasty. METHODS: Human donor corneas (n = 12) with intact endothelium underwent experimental penetrating keratoplasty. Endothelial injury was evaluated after each suture quartile using trypan staining, a validated modality for assessing endothelial injury...
December 8, 2017: Cornea
https://www.readbyqxmd.com/read/29218639/histidine-tracts-in-human-transcription-factors-insight-into-metal-ion-coordination-ability
#4
Aleksandra Hecel, Joanna Wątły, Magdalena Rowińska-Żyrek, Jolanta Świątek-Kozłowska, Henryk Kozłowski
Consecutive histidine repeats are chosen both by nature and by molecular biologists due to their high affinity towards metal ions. Screening of the human genome showed that transcription factors are extremely rich in His tracts. In this work, we examine two of such His-rich regions from forkhead box and MAFA proteins-MB3 (contains 18 His) and MB6 (with 21 His residues), focusing on the affinity and binding modes of Cu2+ and Zn2+ towards the two His-rich regions. In the case of Zn2+ species, the availability of imidazole nitrogen donors enhances metal complex stability...
December 7, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/29216333/a-donor-specific-qtl-exhibiting-allelic-variation-for-leaf-sheath-hairiness-in-a-nested-association-mapping-population-is-located-on-barley-chromosome-4h
#5
Stephanie Saade, Burcu Kutlu, Vera Draba, Karin Förster, Erika Schumann, Mark Tester, Klaus Pillen, Andreas Maurer
Leaf sheath hairiness is a morphological trait associated with various advantages, including tolerance to both abiotic and biotic stresses, thereby increasing yield. Understanding the genetic basis of this trait in barley can therefore improve the agronomic performance of this economically important crop. We scored leaf sheath hairiness in a two-year field trial in 1,420 BC1S3 lines from the wild barley nested association mapping (NAM) population HEB-25. Leaf sheath hairiness segregated in six out of 25 families with the reference parent Barke being glabrous...
2017: PloS One
https://www.readbyqxmd.com/read/29214630/a-del-phenotype-attributed-to-rhd-exon-9-sequence-deletion-slipped-strand-mispairing-and-blood-group-polymorphisms
#6
Genghis H Lopez, Robyn M Turner, Eunike C McGowan, Elizna M Schoeman, Stacy A Scott, Helen O'Brien, Glenda M Millard, Eileen V Roulis, Amanda J Allen, Yew-Wah Liew, Robert L Flower, Catherine A Hyland
BACKGROUND: The RhD blood group antigen is extremely polymorphic and the DEL phenotype represents one such class of polymorphisms. The DEL phenotype prevalent in East Asian populations arises from a synonymous substitution defined as RHD*1227A. However, initially, based on genomic and cDNA studies, the genetic basis for a DEL phenotype in Taiwan was attributed to a deletion of RHD Exon 9 that was never verified at the genomic level by any other independent group. Here we investigate the genetic basis for a Caucasian donor with a DEL partial D phenotype and compare the genomic findings to those initial molecular studies...
December 6, 2017: Transfusion
https://www.readbyqxmd.com/read/29213142/the-attitudes-of-ivf-patients-treated-in-the-czech-republic-towards-informing-children-born-after-gamete-donation
#7
Tatana Rumpikova, Ivana Oborna, Silvie Belaskova, Hana Konecna, David Rumpik
BACKGROUND: In recent decades gamete donation has received growing attention. Data from the Czech National Registry of Assisted Reproduction show that the number of cycles using donated oocytes has been increasing every year. According to Czech law, gamete donation is anonymous. Since 2011, some members of the Czech parliament have repeatedly made requests to revoke the anonymity but anonymity is one of the preconditions for such donation in this country. The aim of this study was to find out how the gamete recipients feel towards informing their child about the circumstances of their conception and their access to the identity of the donor...
December 4, 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/29205775/donor-derived-metastatic-urothelial-cancer-after-kidney-transplantation-associated-with-a-potentially-oncogenic-bk-polyomavirus
#8
David C Müller, Maarit Rämö, Klaudia Naegele, Sebastian Ribi, Christian Wetterauer, Valeria Perrina, Luca Quagliata, Tatjana Vlajnic, Christian Ruiz, Beate Balitzki, Rainer Grobholz, Rainer Gosert, Elvis T Ajuh, Hans H Hirsch, Lukas Bubendorf, Cyrill A Rentsch
BK polyomavirus has been linked to urothelial carcinoma in immunosuppressed patients. Here, we performed comprehensive genomic analysis of a BK polyomavirus -associated, metachronous, multifocal and metastatic micropapillary urothelial cancer in a kidney transplant recipient. Dissecting cancer heterogeneity by sorting technologies prior to array-comparative genomic hybridization followed by short tandem repeat analysis revealed that the metastatic urothelial cancer was of donor-origin (4 year-old male). The top 50 cancer-associated genes displayed no key driver mutations as assessed by next generation sequencing...
December 4, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/29205140/autologous-fat-transplantation-for-secondary-breast-reconstruction-our-experience
#9
F Simonacci, M P Grieco, N Bertozzi, E Raposio
BACKGROUND: Autologous fat transplantation is used after breast reconstruction to improve the breast profile. There are a variety of different methods used for fat harvesting, preparation, and reinjection. This study describes the specific techniques we used in this series of autologous fat transplantations in breast reconstruction patients and reports their outcomes compared with other studies in the literature. PATIENTS AND METHODS: At the University Hospital of Parma between May 2012 and December 2016, we performed 53 autologous fat transplantations for secondary breast reconstruction patients with an average age of 49 years (range: 34-65 y)...
May 2017: Il Giornale di Chirurgia
https://www.readbyqxmd.com/read/29198975/improvement-of-bovine-in%C3%A2-vitro-embryo-production-by-ovarian-follicular-wave-synchronization-prior-to-ovum-pick-up
#10
F L B Cavalieri, F Morotti, M M Seneda, A H B Colombo, M A Andreazzi, I P Emanuelli, L P Rigolon
This study evaluated the effects of the synchronization of ovarian follicular wave emergence on the efficiency of in vitro embryo production. Bos indicus cows (n = 20) were divided into two groups (control vs. synchronization) and subjected to repeated ovum pick-up (OPU) sessions (8 replicates each, with an interval of 21 days in a 2 × 2 crossover design) and subsequent in vitro embryo production. Cows in the control group (n = 10) were submitted to OPU procedures without any stimulation every 21 days...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29190151/the-effect-of-repeated-light-dark-shifts-on-uterine-receptivity-and-early-gestation-in-mice-undergoing-embryo-transfer
#11
Cathy A Goldstein, Louise M O'Brien, Ingrid L Bergin, Thomas L Saunders
Female shift workers are at increased risk for negative reproductive outcomes, and animal evidence suggests that manipulation of the light-dark cycle is detrimental to early gestation in female mice. Specifically, failure of implantation may be responsible for these findings. The objective of this study was to better delineate which reproductive processes are vulnerable to detrimental effects of maternal circadian disturbance. We exposed mice undergoing embryo transfer to repetitive phase advances of the photoperiod...
November 30, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29189423/the-boy-in-the-bubble-and-the-baby-with-the-berlin-heart-the-dangers-of-bridge-to-decision-in-pediatric-mechanical-circulatory-support
#12
Seth A Hollander, Ethan J Hollander
On the day of his birth in 1971, David Vetter was "temporarily" placed in a sterile isolator to wait for a bone marrow donor who would cure his Severe Combined Immunodeficiency Syndrome. After enduring 12 years in isolation, David, now known to the world as "The Boy in the Bubble", received a bone marrow transplant from his unmatched sister and died 4 months later. Like Severe Combined Immunodeficiency Syndrome, pediatric heart failure is a rare and life-threatening condition for which organ transplantation is often the only option for survival...
November 14, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29188051/the-science-behind-autologous-fat-grafting
#13
REVIEW
Elisa Bellini, Michele P Grieco, Edoardo Raposio
Introduction: Adipose grafting has undergone significant changes over time. Many different techniques have been followed by trying to improve the quality of the lipoaspirate and the survival of the fat graft after implantation. Material and methods: The purpose of this review is to analyse the historical evolution of the surgical harvesting and implant technique, describing the changes that have brought significant improvements, revolutionizing the aesthetic and functional results obtainable...
December 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/29179473/a-complex-microsatellite-at-chromosome-7q33-as-a-new-prognostic-marker-of-colorectal-cancer
#14
Xu Ye, Hongyu Deng, Min Su, Qianjin Liao, Dan Huang, Duan-Fang Liao, Zhi-Qiang Xiao, Deliang Cao
Disease-specific markers are critical for early diagnosis, targeted therapy and prognostic prediction of diseases. Current study reports a complex microsatellite as a new prognostic marker of sporadic colorectal cancer. This microsatellite located at Chromosome 7q33 is composed of three tetranucleotide tandem repeats, (TTCC)2(TCCC)5(TCCT)7, flanked by a CT-rich sequence. We analyzed polymorphisms of this microsatellite in 158 sporadic colorectal cancer, 143 matched normal adjacent tissues (NAT) and 150 health donors...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29178242/deceased-organ-donor-screening-for-human-immunodeficiency-virus-hepatitis-b-and-hepatitis-c-viruses-discordant-serology-and-nucleic-acid-testing-results
#15
Nicole Theodoropoulos, Marek J Nowicki, Claudia Chinchilla-Reyes, Sara Dionne, Andrés Jaramillo, Tom Mone, Richard Hasz, Martin D Jendrisak, Daniela P Ladner, Michael G Ison
BACKGROUND: Before the 2014 policy change pertaining to infectious disease screening, many organ procurement organizations (OPOs) were supplementing serologic screening of deceased organ donors with nucleic acid testing (NAT) for human immunodeficiency virus (HIV-1), hepatitis B virus (HBV), and hepatitis C virus (HCV). The number of seronegative, NAT-positive donors has not been directly measured. METHODS: HIV, HBV, and HCV screening results of 11 229 donor referrals evaluated from 2010 to 2013 were obtained from three OPO-affiliated laboratories, capturing 35% of all donors in the United States...
November 27, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29169919/harnessing-the-native-type-i-b-crispr-cas-for-genome-editing-in-a-polyploid-archaeon
#16
Feiyue Cheng, Luyao Gong, Dahe Zhao, Haibo Yang, Jian Zhou, Ming Li, Hua Xiang
Research on CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated protein) systems has led to the revolutionary CRISPR/Cas9 genome editing technique. However, for most archaea and half of bacteria, exploitation of their native CRISPR-Cas machineries may be more straightforward and convenient. In this study, we harnessed the native type I-B CRISPR-Cas system for precise genome editing in the polyploid haloarchaeon Haloarcula hispanica. After testing different designs, the editing tool was optimized to be a single plasmid that carries both the self-targeting mini-CRISPR and a 600-800 bp donor...
November 2, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/29167197/rare-pfcsp-c-terminal-antibodies-induced-by-live-sporozoite-vaccination-are-ineffective-against-malaria-infection
#17
Stephen W Scally, Rajagopal Murugan, Alexandre Bosch, Gianna Triller, Giulia Costa, Benjamin Mordmüller, Peter G Kremsner, B Kim Lee Sim, Stephen L Hoffman, Elena A Levashina, Hedda Wardemann, Jean-Philippe Julien
Antibodies against the central repeat of the Plasmodium falciparum (Pf) circumsporozoite protein (CSP) inhibit parasite activity and correlate with protection from malaria. However, the humoral response to the PfCSP C terminus (C-PfCSP) is less well characterized. Here, we describe B cell responses to C-PfCSP from European donors who underwent immunization with live Pf sporozoites (PfSPZ Challenge) under chloroquine prophylaxis (PfSPZ-CVac), and were protected against controlled human malaria infection. Out of 215 PfCSP-reactive monoclonal antibodies, only two unique antibodies were specific for C-PfCSP, highlighting the rare occurrence of C-PfCSP-reactive B cells in PfSPZ-CVac-induced protective immunity...
November 22, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29165235/synthesis-of-n-acetyl-d-quinovosamine-in-rhizobium-etli-ce3-is-completed-after-its-4-keto-precursor-is-linked-to-a-carrier-lipid
#18
Tiezheng Li, K Dale Noel
Bacterial O-antigens are synthesized on lipid carriers before being transferred to lipopolysaccharide core structures. Rhizobium etli CE3 lipopolysaccharide is a model for understanding O-antigen biological function. CE3 O-antigen structure and genetics are known. However, proposed enzymology for CE3 O-antigen synthesis has been examined very little in vitro, and even the sugar added to begin the synthesis is uncertain. A model based on mutagenesis studies predicts that 2-acetamido-2,6-dideoxy-d-glucose (QuiNAc) is the first O-antigen sugar and that genes wreV, wreQ and wreU direct QuiNAc synthesis and O-antigen initiation...
November 22, 2017: Microbiology
https://www.readbyqxmd.com/read/29159585/kfoa-the-udp-glucose-4-epimerase-of-escherichia-coli-strain-o5-k4-h4-shows-preference-for-acetylated-substrates
#19
Hao-Miao Zhu, Bin Sun, Yi-Jun Li, Dan-Hua Meng, Shuang Zheng, Ting-Ting Wang, Feng-Shan Wang, Ju-Zheng Sheng
Capsule of Escherichia coli O5:K4:H4 is formed of a chondroitin-repeat disaccharide unit of glucuronic acid (GlcA)-N-acetylgalactosamine (GalNAc). This polysaccharide, commonly referred to as K4CP, is a potentially important source of precursors for chemoenzymatic or bioengineering synthesis of chondroitin sulfate. KfoA, encoded by a gene from region 2 of the K4 capsular gene cluster, shows high homology to the UDP-glucose-4-epimerase (GalE) from E. coli. KfoA is reputed to be responsible for uridine 5'-diphosphate-N-acetylgalactosamine (UDP-GalNAc) supply for K4CP biosynthesis in vivo, but it has not been biochemically characterized...
November 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29149954/preoperative-risk-factors-associated-with-urinary-complications-after-kidney-transplantation
#20
T Bessede, Y Hammoudi, D Bedretdinova, B Parier, H Francois, A Durrbach, G Benoit
BACKGROUND: Only surgically irrelevant risk factors including sex, African-American ancestry, or exceptional U-stitch anastomosis have been identified to associate with urinary complications after kidney transplantation. The objective was to identify modifiable and nonmodifiable risk factors associated with urinary complications after kidney transplantation. METHODS: A single-center study of 3,129 kidney transplants performed over 40 years was conducted to identify independent risk factors using χ(2) tests and logistic regression analysis...
November 2017: Transplantation Proceedings
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