keyword
MENU ▼
Read by QxMD icon Read
search

repeat donors

keyword
https://www.readbyqxmd.com/read/28229804/immediately-functioning-grafts-from-brain-dead-donors-treated-with-extracorporeal-membrane-oxygenation
#1
Faissal A M Shaheen, Mohamed Shalabi, Ahmed Zain, Mahmood Hasanain, Abdulla Al Sayyari
A brain-dead donor experienced repeated cardiac arrests followed by severe hypotension requiring multiple vasoactive agents. These events were associated with severe lactic acidosis and dysregulated kidney function in the donor. A 10-hour treatment with extracorporeal membranous oxygenation was instituted, which was able to hemodynamically stabilize the donor. This treatment protocol resulted in the procurement of 2 viable kidney grafts transplanted into 2 recipients, who had immediate kidney graft function and excellent serum creatinine levels upon hospital discharge...
February 23, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28225830/ex-vivo-activation-of-cd4-t-cells-from-donors-on-suppressive-art-can-lead-to-sustained-production-of-infectious-hiv-1-from-a-subset-of-infected-cells
#2
John K Bui, Elias K Halvas, Elizabeth Fyne, Michele D Sobolewski, Dianna Koontz, Wei Shao, Brian Luke, Feiyu F Hong, Mary F Kearney, John W Mellors
The fate of HIV-infected cells after reversal of proviral latency is not well characterized. Simonetti, et al. recently showed that CD4+ T-cells containing intact proviruses can clonally expand in vivo and produce low-level infectious viremia. We hypothesized that reversal of HIV latency by activation of CD4+ T-cells can lead to the expansion of a subset of virus-producing cells rather than their elimination. We established an ex vivo cell culture system involving stimulation of CD4+ T-cells from donors on suppressive antiretroviral therapy (ART) with PMA/ionomycin (day 1-7), followed by rest (day 7-21), and then repeat stimulation (day 21-28), always in the presence of high concentrations of raltegravir and efavirenz to effectively block new cycles of viral replication...
February 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28223970/tet-c-gene-transfer-between-chlamydia-suis-strains-occurs-by-homologous-recombination-after-co-infection-implications-for-spread-of-tetracycline-resistance-among-chlamydiaceae
#3
Hanna Marti, Hoyon Kim, Sandeep J Joseph, Stacey Dojiri, Timothy D Read, Deborah Dean
Chlamydia suis is a swine pathogen that has also recently been found to cause zoonotic infections of the human eye, pharynx, and gastrointestinal tract. Many strains contain a tetracycline class C gene [tet(C)] cassette that confers tetracycline resistance. The cassette was likely originally acquired by horizontal gene transfer from a Gram-negative donor after the introduction of tetracycline into animal feed in the 1950s. Various research groups have described the capacity for different Chlamydia species to exchange DNA by homologous recombination...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28218010/changes-in-hematological-indices-and-lymphocyte-subsets-in-response-to-whole-blood-donation-in-healthy-male-donors
#4
Anwar Borai, Callum Livingstone, Enaam Alsobhi, Abeer Al Sofyani, Dalal Balgoon, Anwar Farzal, Mohammed Almohammadi, Abdulafattah Al-Amri, Suhad Bahijri, Daad Alrowaili, Wafaa Bassiuni, Ayman Saleh, Norah Alrowaili, Mohamed Abdelaal
Whole blood donation has immunomodulatory effects, and most of these have been observed at short intervals following blood donation. This study aimed to investigate the impact of whole blood donation on lymphocyte subsets over a typical inter-donation interval. Healthy male subjects were recruited to study changes in complete blood count (CBC) (n = 42) and lymphocyte subsets (n = 16) before and at four intervals up to 106 days following blood donation. Repeated measures ANOVA were used to compare quantitative variables between different visits...
April 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28215840/cytidine-deaminase-apobec3a-induction-in-fallopian-epithelium-after-exposure-to-follicular-fluid
#5
Pavla Brachova, Nehemiah S Alvarez, Bradley J Van Voorhis, Lane K Christenson
OBJECTIVE: Ovarian carcinomas that originate from fallopian epithelial cells are suggested to arise due to repeated exposure to ovulatory follicular fluid (FF). Mechanistic explanation(s) for how this occurs are unknown. Here, we sought to understand if FF exposure to fallopian epithelial cells could induce DNA damage and expression of a known family of DNA mutators, apolipoprotein B mRNA editing enzyme, catalytic polypeptide (APOBEC) cytidine deaminases. METHODS: Follicular fluid and matched patient plasma samples were obtained from donors...
February 16, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28215057/efficacy-of-human-platelet-rich-fibrin-exudate-vs-fetal-bovine-serum-on-proliferation-and-differentiation-of-dental-pulp-stem-cells
#6
Mahmoud A Saeed, Mohamed Abd El-Rahman, Mohamed E Helal, Ahmed R Zaher, Mohammed E Grawish
Background and Objectives: The imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands an in-vitro expansion which must deal with the safety and ethical problems associated with fetal bovine serum (FBS). The primary aim of this study was to compare the effects of human platelet rich fibrin (hPRF) exudate Vs FBS on proliferation and osteodifferentiation of human dental pulp stem cells (hDPSCs). The secondary one was to determine the optimum concentration of hPRF exudate inducing hDPSCs proliferation and osteodifferentiation...
February 28, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28210266/a-phylogenetic-analysis-of-chloroplast-genomes-elucidates-the-relationships-of-the-six-economically-important-brassica-species-comprising-the-triangle-of-u
#7
Peirong Li, Shujiang Zhang, Fei Li, Shifan Zhang, Hui Zhang, Xiaowu Wang, Rifei Sun, Guusje Bonnema, Theo J A Borm
The Brassica genus comprises many economically important worldwide cultivated crops. The well-established model of the Brassica genus, U's triangle, consists of three basic diploid plant species (Brassica rapa, Brassica oleracea, and Brassica nigra) and three amphidiploid species (Brassica napus, Brassica juncea, and Brassica carinata) that arose through interspecific hybridizations. Despite being extensively studied because of its commercial relevance, several aspects of the origin of the Brassica species and the relationships within and among these six species still remain open questions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196473/2-7-million-samples-genotyped-for-hla-by-next-generation-sequencing-lessons-learned
#8
Gerhard Schöfl, Kathrin Lang, Philipp Quenzel, Irina Böhme, Jürgen Sauter, Jan A Hofmann, Julia Pingel, Alexander H Schmidt, Vinzenz Lange
BACKGROUND: At the DKMS Life Science Lab, Next Generation Sequencing (NGS) has been used for ultra-high-volume high-resolution genotyping of HLA loci for the last three and a half years. Here, we report on our experiences in genotyping the HLA, CCR5, ABO, RHD and KIR genes using a direct amplicon sequencing approach on Illumina MiSeq and HiSeq 2500 instruments. RESULTS: Between January 2013 and June 2016, 2,714,110 samples largely from German, Polish and UK-based potential stem cell donors have been processed...
February 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28192074/practical-informativeness-of-short-tandem-repeat-loci-for-chimerism-analysis-in-hematopoietic-stem-cell-transplantation
#9
Eunhee Han, Myungshin Kim, Yonggoo Kim, Kyungja Han, Jihyang Lim, Dain Kang, Gun Dong Lee, Jung Rok Kim, Jae-Wook Lee, Nack-Gyun Chung, Bin Cho, Ki-Seong Eom, Yoo-Jin Kim, Hee-Je Kim, Seok Lee, Seok-Goo Cho, Chang-Ki Min, Dong-Wook Kim, JongWook Lee, Woo-Sung Min
OBJECTIVE: Short tandem repeat (STR) loci are most frequently used for chimerism analysis after hematopoietic stem cell transplantation (HSCT). The aim of this study was to evaluate the practical informativeness of STR chimerism by integrating theoretical and analytical points. METHODS: Theoretical and practical informativess of 16 STR loci were evaluated from 1249 pairs of recipients and donors who were prepared for HSCT. RESULTS: Theoretical informativeness was influenced by genetic diversity including allele frequency and heterozygosity, and was higher in the unrelated HSCT group (90...
February 10, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28188872/pre-transplantation-31-p-magnetic-resonance-spectroscopy-for-quality-assessment-of-human-pancreatic-grafts-a-feasibility-study
#10
Lina Carlbom, Jan Weis, Lars Johansson, Olle Korsgren, Håkan Ahlström
OBJECTIVE: To investigate the feasibility of using (31)P-MRS for objective non-invasive quality assessment of human pancreas grafts prior to transplantation or islet isolation. MATERIALS AND METHODS: Pancreata from 5 human donors, 3 males and 2 females, aged 49-78years, with body mass index (BMI) 22-31kg/m(2), were included. Pancreata were perfused with histidine-tryptophan-ketoglutarate solution during procurement and stored in hypothermic condition (4°C) for 21-44h...
February 8, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28186648/the-recipient-s-heme-oxygenase-1-promoter-region-polymorphism-is-associated-with-cardiac-allograft-vasculopathy
#11
Kathrin Freystaetter, Martin Andreas, Martin Bilban, Thomas Perkmann, Alexandra Kaider, Marco Masetti, Alfred Kocher, Michael Wolzt, Andreas Zuckermann
BACKGROUND: Heme oxygenase-1 (HO-1) catalyses the degradation of heme to biliverdin, free iron and carbon monoxide. The promoter region contains a highly polymorphic (GT)n repeat, where shorter (GT)n repeat sequences are linked to higher transcriptional activity, which was shown to correlate with a cytoprotective effect. Higher HO-1 levels may protect from cardiac allograft vasculopathy. METHODS: Cardiac allograft recipients transplanted between 1988 and 2012 were analysed for the HO-1 (GT)n repeat polymorphism using PCR and DNA fragment analysis with capillary electrophoresis...
February 10, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28186611/-ethical-issues-of-oocyte-donation
#12
Yanjin Liu, Libai Cai
The birth and development of oocyte donation technology have brought hope for women with poor ovarian reserve and repeated failure for in vitro fertilization, as well as for those with chromosomal abnormalities, premature ovarian failure, or at perimenopausal or menopausal stages. It has not only preserved their reproductive right, but also stabilized their families and increased social harmony. However, this technology does not only involve infertile couples themselves, but also social and ethical issues concerning their families and the society...
February 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28182823/how-to-direct-the-neuronal-growth-process-in-peripheral-nerve-regeneration-future-strategies-for-nanosurfaces-scaffold-and-magnetic-nanoparticles
#13
Andrea Poggetti, Pietro Battistini, Paolo Domenico Parchi, Michela Novelli, Simona Raffa, Marco Cecchini, Anna Maria Nucci, Michele Lisanti
Currently, the gold standard to repair large nerve defects is the autologous nerve graft. These solutions offer a mechanical support, adhesion substrates, and, with Schwann cells (SC), a source of neurotropic factors for axonal growth. The technical limits are the donor side damage, multiple surgical accesses, and the unavailability of large amounts of grafts. In recent years, several researchers focused their attention on the interaction between cells (nervous and glial) and physic-chemical cues that arise from the extracellular milieu...
February 7, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/28177972/sustained-overcorrection-after-autologous-facial-fat-grafting-in-the-pediatric-population-a-case-series
#14
Kathryn Ries Tringale, Samuel Lance, Anna Schoenbrunner, Amanda A Gosman
INTRODUCTION: Autologous fat grafting is a valuable tool in the correction of facial soft tissue asymmetry and volume deficits. Pubertal growth and fluctuations in body mass present unique challenges to achieving satisfactory results after autologous fat transfer in the pediatric population. Few studies exist describing the outcomes and complications of pediatric facial fat grafting. The objective of this study is to identify the complication profile and outcomes after autologous fat grafting for the correction of facial asymmetry and volume deficits in the pediatric population...
February 8, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28169563/collinearity-between-potato-solanum-tuberosum-l-and-wild-relatives-assessed-by-comparative-cytogenetic-mapping
#15
Paola Gaiero, José van de Belt, Francisco Vilaró, M Eric Schranz, Pablo Speranza, Hans de Jong
A major bottleneck to introgressive hybridization is the lack of genome collinearity between the donor (alien) genome and the recipient crop genome. Structural differences between the homeologs may create unbalanced segregation of chromosomes or cause linkage drag. To assess large-scale collinearity between potato and two of its wild relatives (Solanum commersonii and Solanum chacoense), we used BAC-FISH mapping of sequences with known positions on the RH potato map. BAC probes could successfully be hybridized to the S...
November 14, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28166988/beef-donor-cows-with-high-number-of-retrieved-coc-produce-more-in%C3%A2-vitro-embryos-compared-with-cows-with-low-number-of-coc-after-repeated-ovum-pick-up-sessions
#16
F M Monteiro, E O S Batista, L M Vieira, B M Bayeux, M Accorsi, S P Campanholi, E A R Dias, A H Souza, P S Baruselli
The aim of the present study was to evaluate whether repeated ovum pick-up (OPU) procedures may affect the efficiency of OPU programs for in vitro embryo production (IVEP) in Bos indicus (Nelore) donors. In addition, the repeatability (r) efficiency of IVEP was also assessed. Data available were from 432 OPU-IVEP sessions that were performed at random stages of the estrous cycle in 36 cycling, nonlactating Nelore donors. Semen from three Nelore bulls was used for the IVF. Donors were submitted to 12 consecutive OPU procedures, with an interval of approximately 30 days between sessions...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28165201/electromembrane-extraction-and-preconcentration-of-carbendazim-and-thiabendazole-in-water-samples-before-capillary-electrophoresis-analysis
#17
Amilton Moreira Oliveira, Hugo Campos Loureiro, Francielly Fernanda Souza Jesus, Dosil Pereira de Jesus
Electromembrane extraction using a polypropylene hollow fiber impregnated with 1-ethyl-2-nitrobenzene was evaluated for the extraction and preconcentration of the fungicides thiabendazole and carbendazim from water samples before capillary electrophoresis analysis. The composition of the supported liquid membrane, the HCl concentration in the acceptor solution, and the stirring rate (of the donor solution) were optimized using the one-variable-at-a-time method. In contrast, a face-centered central composition design was used for optimization of voltage, extraction time, and concentration of HCl in the donor solution...
February 6, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28164344/qualitative-assessment-of-pica-experienced-by-frequent-blood-donors
#18
Melanie C Chansky, Melissa R King, Walter Bialkowski, Barbara J Bryant, Joseph E Kiss, Pam D'Andrea, Ritchard G Cable, Bryan R Spencer, Alan E Mast
BACKGROUND: Pica, the compulsive consumption of ice or other nonnutritious substances, is associated with iron deficiency, a common negative consequence of frequent blood donation. Because of this, blood donors, such as those participating in the Strategies to Reduce Iron Deficiency (STRIDE) study, are an ideal population to explore pica and iron deficiency. STUDY DESIGN AND METHODS: STRIDE was a 2-year intervention trial to assess the effectiveness of iron supplementation for mitigating iron deficiency in frequent blood donors...
February 5, 2017: Transfusion
https://www.readbyqxmd.com/read/28164313/the-characteristics-of-hepatitis%C3%A2-b-surface-antigen-hbsag-negative-hepatitis%C3%A2-b-virus-hbv-infection-in-chinese-blood-donors-a-follow-up-study-of-donors-tested-negative-for-hbsag-and-reactive-for-simultaneous-nucleic-acid-testing-of-hbv-hepatitis%C3%A2-c-virus-and
#19
Zhaofu Guo, Ping Fu, Yijin Yin, Funeng Wang, Yiqing Yin, Jingxing Wang, Yu Liu
BACKGROUND: The real infection status of hepatitis B virus (HBV) of hepatitis B surface antigen (HBsAg)-negative yet nucleic acid test (NAT)-positive blood donors is difficult to clarify. Detailed follow-up study is needed for analyzing the infectivity of these blood donors. STUDY DESIGN AND METHODS: Blood donors who screened negative for HBsAg and reactive for simultaneous NAT of HBV, hepatitis C virus (HCV), and human immunodeficiency virus (HIV) were included in a follow-up epidemiologic questionnaire survey and contributed follow-up samples for further testing...
February 5, 2017: Transfusion
https://www.readbyqxmd.com/read/28159550/characterization-of-the-diversity-of-t-cell-receptor-%C3%AE-%C3%AE-complementary-determinant-region-3-in-human-peripheral-blood-by-immune-repertoire-sequencing
#20
Hui Chen, Mingjin Zou, Da Teng, Jianmin Zhang, Wei He
γδ T cells function as sentinels in early host response to infections and malignancies. Although γδ T cells are regarded as innate immune cells and recognize antigens in a non-MHC restricted manner, they possess a huge diversity of complementary determinant region 3 (CDR3) of T cell receptor (TCR) generated by the rearrangement of germ-line gene V- (D) -J-C fragments. However, the detailed characteristics of the TCRγδ CDR3 repertoire remain unclear. A comprehensive analysis would answer fundamental questions about the diversity of the TCRγδ CDR3 repertoire and elucidate the mechanism underlying γδ T cell recognition of pathogens and tumor antigens...
January 31, 2017: Journal of Immunological Methods
keyword
keyword
62896
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"